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  1. Decades of research is available on their effects of single component surfactant on active ingredient diffusion across plant cuticular membranes, but ingredient diffusion is rarely analysed in the presence of ...

    Authors: Lakshmi Venkatesha Manyu Vittal, James Rookes, Ben Boyd and David Cahill
    Citation: Plant Methods 2023 19:37
  2. Stomata are tiny pores on the leaf surface that are central to gas exchange. Stomatal number, size and aperture are key determinants of plant transpiration and photosynthesis, and variation in these traits can...

    Authors: Phetdalaphone Pathoumthong, Zhen Zhang, Stuart J. Roy and Abdeljalil El Habti
    Citation: Plant Methods 2023 19:36
  3. Cell characteristics, including cell type, size, shape, packing, cell-to-cell-adhesion, intercellular space, and cell wall thickness, influence the physical characteristics of plant tissues. Genotypic differen...

    Authors: Nelia Nause, Facundo R. Ispizua Yamati, Marion Seidel, Anne-Katrin Mahlein and Christa M. Hoffmann
    Citation: Plant Methods 2023 19:35
  4. Insertion of Mg2+ into protoporphyrin IX (PPIX) to produce magnesium-protoporphyrin IX (Mg-PPIX) was the first step toward chlorophyll biosynthesis, which not only imparts plants green pigmentation but underpins ...

    Authors: Chenyu Zhang, Chunlei Ma, Li Zhu and Mingzhe Yao
    Citation: Plant Methods 2023 19:34
  5. Plant architecture can influence crop yield and quality. Manual extraction of architectural traits is, however, time-consuming, tedious, and error prone. The trait estimation from 3D data addresses occlusion i...

    Authors: Farah Saeed, Shangpeng Sun, Javier Rodriguez-Sanchez, John Snider, Tianming Liu and Changying Li
    Citation: Plant Methods 2023 19:33
  6. Necrotizing pathogens pose an immense economic and ecological threat to trees and forests, but the molecular analysis of these pathogens is still in its infancy because of lacking model systems. To close this ...

    Authors: Steven Dreischhoff, Ishani Shankar Das, Felix Häffner, Anna Malvine Wolf, Andrea Polle and Karl Henrik Kasper
    Citation: Plant Methods 2023 19:32
  7. Even for easy-to-transform species or genotypes, the creation of transgenic or edited plant lines remains a significant bottleneck. Thus, any technical advance that accelerates the regeneration and transformat...

    Authors: Camille Soulhat, Houssein Wehbi, Yannick Fierlej, Patrick Berquin, Thomas Girin, Pierre Hilson and Oumaya Bouchabké-Coussa
    Citation: Plant Methods 2023 19:31
  8. In plant genome editing, RNA-guided nucleases such as Cas9 from Streptococcus pyogenes (SpCas9) predominantly induce small insertions or deletions at target sites. This can be used for inactivation of protein-cod...

    Authors: Jana Ordon, Niklas Kiel, Dieter Becker, Carola Kretschmer, Paul Schulze-Lefert and Johannes Stuttmann
    Citation: Plant Methods 2023 19:30
  9. Remote sensing instruments enable high-throughput phenotyping of plant traits and stress resilience across scale. Spatial (handheld devices, towers, drones, airborne, and satellites) and temporal (continuous o...

    Authors: Christopher Y. S. Wong, Taylor Jones, Devin P. McHugh, Matthew E. Gilbert, Paul Gepts, Antonia Palkovic, Thomas N. Buckley and Troy S. Magney
    Citation: Plant Methods 2023 19:29
  10. Selection is one of the essential skills whereby breeders reduce the population size and increase the chance of success. Various selection methods with special applications have been developed. Superior genoty...

    Authors: Peyman Eynizadeh, Hamid Dehghani and Ali Dehghani
    Citation: Plant Methods 2023 19:25
  11. As one of the largest drupes in the world, the coconut has a special multilayered structure and a seed development process that is not yet fully understood. On the one hand, the special structure of the coconu...

    Authors: Yu Zhang, Qianfan Liu, Jing Chen, Chengxu Sun, Shenghuang Lin, Hongxing Cao, Zhaolin Xiao and Mengxing Huang
    Citation: Plant Methods 2023 19:24
  12. The rapidly advancing corn breeding field calls for high-throughput methods to phenotype corn kernel traits to estimate yield and to study their genetic inheritance. Most of the existing methods are reliant on...

    Authors: Samantha Gillette, Lu Yin, Penny M. A. Kianian, Wojciech P. Pawlowski and Changbin Chen
    Citation: Plant Methods 2023 19:23
  13. Root-knot nematodes (RKNs) pose a worldwide threat to agriculture of many crops including cucumber. Genetic transformation (GT) has emerged as a powerful tool for exploration of plant-RKN interactions and gene...

    Authors: Xu Zhang, Shihui Li, Xin Li, Mengyuan Song, Si Ma, Yongqiang Tian and Lihong Gao
    Citation: Plant Methods 2023 19:22
  14. Various growth systems are available for studying plant root growth and plant–microbe interactions including hydroponics and aeroponics. Although some of these systems work well with Arabidopsis thaliana and smal...

    Authors: Jingya Cai, Vijaykumar Veerappan, Kate Arildsen, Catrina Sullivan, Megan Piechowicz, Julia Frugoli and Rebecca Dickstein
    Citation: Plant Methods 2023 19:21

    The Correction to this article has been published in Plant Methods 2023 19:28

  15. Bamboo is a perennial and renewable biomass forest resource and its leaf flavonoid is an antioxidant for biological and pharmacological research. The established genetic transformation and gene editing systems...

    Authors: Huayu Sun, Sining Wang, Chenglei Zhu, Kebin Yang, Yan Liu and Zhimin Gao
    Citation: Plant Methods 2023 19:20
  16. Non-invasive reporter systems are powerful tools to query physiological and transcriptional responses in organisms. For example, fluorescent and bioluminescent reporters have revolutionized cellular and organi...

    Authors: Maria L. Sorkin, Kathleen K Markham, Stevan Zorich, Ananda Menon, Kristen N. Edgeworth, Angela Ricono, Douglas Bryant, Rebecca Bart, Dmitri A. Nusinow and Kathleen Greenham
    Citation: Plant Methods 2023 19:19
  17. Lignocellulosic biomass is a complex network of polysaccharides and lignin that requires a pretreatment step to overcome recalcitrance and optimize valorisation into biobased products. Pretreatment of biomass ...

    Authors: Edwige Audibert, Berangère Lebas, Corentin Spriet, Anouck Habrant, Brigitte Chabbert and Gabriel Paës
    Citation: Plant Methods 2023 19:16
  18. Agrobacterium tumefaciens-mediated leaf disc genetic transformation is an important way to achieve transgenics or gene editing. Ensuring stable and efficient genetic transformation is still an important problem i...

    Authors: Yufei Xia, Yuan Cao, Yongyu Ren, Aoyu Ling, Kang Du, Yun Li, Jun Yang and Xiangyang Kang
    Citation: Plant Methods 2023 19:15
  19. Herbicide resistance is one of the threats to modern agriculture and its early detection is one of the most effective components for sustainable resistance management strategies. Many techniques have been used...

    Authors: Silvia Panozzo, Silvia Farinati, Maurizio Sattin and Laura Scarabel
    Citation: Plant Methods 2023 19:14
  20. Despite the increased efficiency of sequencing technologies and the development of reduced-representation sequencing (RRS) approaches allowing high-throughput sequencing (HTS) of multiplexed samples, the per-s...

    Authors: Maxime de Ronne, Gaétan Légaré, François Belzile, Brian Boyle and Davoud Torkamaneh
    Citation: Plant Methods 2023 19:13
  21. Microscopic analysis of plant anatomy is a common procedure in biology to study structure and function that requires high-quality sections for accurate measurements. Hand sectioning of specimens is typically l...

    Authors: David J. Thomas, Jordan Rainbow and Laura E. Bartley
    Citation: Plant Methods 2023 19:12
  22. Crop breeding based on root system architecture (RSA) optimization is an essential factor for improving crop production in developing countries. Identification, evaluation, and selection of root traits of soil...

    Authors: Qian Wu, Jie Wu, Pengcheng Hu, Weixin Zhang, Yuntao Ma, Kun Yu, Yan Guo, Jing Cao, Huayong Li, Baiming Li, Yuyang Yao, Hongxin Cao and Wenyu Zhang
    Citation: Plant Methods 2023 19:11
  23. The number of soybean pods is one of the most important indicators of soybean yield, pod counting is crucial for yield estimation, cultivation management, and variety breeding. Counting pods manually is slow a...

    Authors: Shuai Xiang, Siyu Wang, Mei Xu, Wenyan Wang and Weiguo Liu
    Citation: Plant Methods 2023 19:8

    The Correction to this article has been published in Plant Methods 2023 19:45

  24. Karst vegetation is of great significance for ecological restoration in karst areas. Vegetation Indices (VIs) are mainly related to plant yield which is helpful to understand the status of ecological restorati...

    Authors: Wen Pan, Xiaoyu Wang, Yan Sun, Jia Wang, Yanjie Li and Sheng Li
    Citation: Plant Methods 2023 19:7
  25. As a result of the technological progress, the use of sensors for crop survey has substantially increased, generating valuable information for modelling agricultural data. Plant spectroscopy jointly with stati...

    Authors: Rosa Angela Pacheco-Gil, Ciro Velasco-Cruz, Paulino Pérez-Rodríguez, Juan Burgueño, Sergio Pérez-Elizalde, Francelino Rodrigues, Ivan Ortiz-Monasterio, David Hebert del Valle-Paniagua and Fernando Toledo
    Citation: Plant Methods 2023 19:6
  26. Cotton has tremendous economic value worldwide; however, its allopolyploid nature and time-consuming transformation methods have hampered the development of cotton functional genomics. The protoplast system ha...

    Authors: Ke Zhang, Shanhe Liu, Yunze Fu, Zixuan Wang, Xiubo Yang, Wenjing Li, Caihua Zhang, Dongmei Zhang and Jun Li
    Citation: Plant Methods 2023 19:5
  27. Cassava is the most cultivated and consumed root crop in the world. One of the major constraints to the cassava value chain is the short shelf life of cassava storage roots which is primarily due to the so-cal...

    Authors: Ima M. Zainuddin, Brieuc Lecart, Enny Sudarmonowati and Hervé Vanderschuren
    Citation: Plant Methods 2023 19:4
  28. This study presents a methodology for a high-throughput digitization and quantification process of plant cell walls characterization, including the automated development of two-dimensional finite element model...

    Authors: Andrew Sayad, Yusuf Oduntan, Norbert Bokros, Seth DeBolt, Alice Benzecry, Daniel J. Robertson and Christopher J. Stubbs
    Citation: Plant Methods 2023 19:3
  29. NADPH oxidase (Nox) plays a crucial role in reactive oxygen spices (ROS) production and mediates different diseases’ development. Under aerobic conditions, the NADPH-containing protein component of the (NCP)-F...

    Authors: Sona M. Feschyan, Ruzan M. Simonyan, Gegham M. Simonyan, Maxim A. Simonyan and Ashkhen L. Manukyan
    Citation: Plant Methods 2023 19:1
  30. In June 2020, severe symptoms of leaf mosaic and fruit malformation were observed on greenhouse-grown cucumber plants in Xizhou Township of Changhua County, Taiwan. An unknown virus, designated CX-2, was isola...

    Authors: Zi-Xuan Dong, Chian-Chi Lin, Yuh-Kun Chen, Chia-Cheng Chou and Tsung-Chi Chen
    Citation: Plant Methods 2022 18:143
  31. Camellia oleifera (C. oleifera) is a woody edible oil crop of great economic importance. Because of the lack of modern biotechnology research, C. oleifera faces huge challenges in both breeding and basic researc...

    Authors: Sufang Li, Rui Zhao, Tianwen Ye, Rui Guan, Linjie Xu, Xiaoling Ma, Jiaxi Zhang, Shixin Xiao and Deyi Yuan
    Citation: Plant Methods 2022 18:141
  32. “Herbarium X-ray Fluorescence (XRF) Ionomics” is a new quantitative approach for extracting the elemental concentrations from herbarium specimens using handheld XRF devices. These instruments are principally d...

    Authors: Imam Purwadi, Lachlan W. Casey, Chris G. Ryan, Peter D. Erskine and Antony van der Ent
    Citation: Plant Methods 2022 18:139
  33. Virtual plants can simulate the plant growth and development process through computer modeling, which assists in revealing plant growth and development patterns. Virtual plant visualization technology is a cor...

    Authors: Lingfeng Duan, Zhihao Wang, Hongfei Chen, Jinyang Fu, Hanzhi Wei, Zedong Geng and Wanneng Yang
    Citation: Plant Methods 2022 18:138
  34. Long-read sequencing platforms offered by Oxford Nanopore Technologies (ONT) allow native DNA containing epigenetic modifications to be directly sequenced, but can be limited by lower per-base accuracies. A ke...

    Authors: Scott Ferguson, Todd McLay, Rose L. Andrew, Jeremy J. Bruhl, Benjamin Schwessinger, Justin Borevitz and Ashley Jones
    Citation: Plant Methods 2022 18:137
  35. Frequency is essential in signal transmission, especially in convolutional neural networks. It is vital to maintain the signal frequency in the neural network to maintain the performance of a convolutional neu...

    Authors: Jiayu Zhang, Chao Qi, Peter Mecha, Yi Zuo, Zongyou Ben, Haolu Liu and Kunjie Chen
    Citation: Plant Methods 2022 18:136
  36. Cyst nematodes are one of the major groups of plant-parasitic nematode, responsible for considerable crop losses worldwide. Improving genetic resources, and therefore resistant cultivars, is an ongoing focus o...

    Authors: Olaf Prosper Kranse, Itsuhiro Ko, Roberta Healey, Unnati Sonawala, Siyuan Wei, Beatrice Senatori, Francesco De Batté, Ji Zhou and Sebastian Eves-van den Akker
    Citation: Plant Methods 2022 18:134
  37. Root system architecture (RSA) is an essential characteristic for efficient water and nutrient absorption in terrestrial plants; its plasticity enables plants to respond to different soil environments. Better ...

    Authors: Shota Teramoto and Yusaku Uga
    Citation: Plant Methods 2022 18:133
  38. Copy number determination is one of the first steps in the characterization of transgenic plant lines. The classical approach to this, Southern blotting, is time-consuming, expensive and requires massive amoun...

    Authors: Jonas De Saeger, Jihae Park, Kai Thoris, Charlotte De Bruyn, Hoo Sun Chung, Dirk Inzé and Stephen Depuydt
    Citation: Plant Methods 2022 18:132
  39. High-throughput phenotyping platforms allow the study of the form and function of a large number of genotypes subjected to different growing conditions (GxE). A number of image acquisition and processing pipel...

    Authors: Benoit Daviet, Romain Fernandez, Llorenç Cabrera-Bosquet, Christophe Pradal and Christian Fournier
    Citation: Plant Methods 2022 18:130

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