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  1. Agrobacterium rhizogenes-mediated hairy root transformation provides a powerful tool for investigating the functions of plant genes involved in rhizobia-legume symbiosis. However, in the traditional identificatio...

    Authors: Yinglun Fan, Xiuyuan Wang, Haiyun Li, Shuang Liu, Liangshen Jin, Yanyan Lyu, Mengdi Shi, Sirui Liu, Xinyue Yang and Shanhua Lyu

    Citation: Plant Methods 2020 16:94

    Content type: Methodology

    Published on:

  2. Genetically encoded fluorescent proteins are often used to label proteins and study protein function and localization in vivo. Traditional cloning methods mediated by restriction digestion and ligation are tim...

    Authors: Liu Zhang, Yang Zhao, Haiyan Liang, Xugang Li, Kimberly L. Gallagher and Shuang Wu

    Citation: Plant Methods 2020 16:93

    Content type: Methodology

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  3. Photosynthesis of reproductive organs in C3 cereals is generally regarded as important to crop yield. Whereas, photosynthetic characteristics of reproductive organs are much less understood as compared to leaf ph...

    Authors: Tian-Gen Chang, Qing-Feng Song, Hong-Long Zhao, Shuoqi Chang, Changpeng Xin, Mingnan Qu and Xin-Guang Zhu

    Citation: Plant Methods 2020 16:92

    Content type: Methodology

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  4. The knowledge of vine vegetative status within a vineyard plays a key role in canopy management in order to achieve a correct vine balance and reach the final desired yield/quality. Detailed information about ...

    Authors: Salvatore Filippo Di Gennaro and Alessandro Matese

    Citation: Plant Methods 2020 16:91

    Content type: Methodology

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  5. Deeper roots help plants take up available resources in deep soil ensuring better growth and higher yields under conditions of drought. A large-scale semi-field root phenotyping facility was developed to allow...

    Authors: Xiangyu Guo, Simon F. Svane, Winnie S. Füchtbauer, Jeppe R. Andersen, Just Jensen and Kristian Thorup-Kristensen

    Citation: Plant Methods 2020 16:90

    Content type: Research

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  6. Root system architecture and especially its plasticity in acclimation to variable environments play a crucial role in the ability of plants to explore and acquire efficiently soil resources and ensure plant pr...

    Authors: Kerstin A. Nagel, Henning Lenz, Bernd Kastenholz, Frank Gilmer, Andreas Averesch, Alexander Putz, Kathrin Heinz, Andreas Fischbach, Hanno Scharr, Fabio Fiorani, Achim Walter and Ulrich Schurr

    Citation: Plant Methods 2020 16:89

    Content type: Methodology

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  7. The legume cowpea (Vigna unguiculata L.) is extensively grown in sub-Saharan Africa. Cowpea, like many legumes has proved recalcitrant to plant transformation. A rapid transient leaf assay was developed for testi...

    Authors: Martina Juranić, Dilrukshi S. K. Nagahatenna, Rigel Salinas-Gamboa, Melanie L. Hand, Nidia Sánchez-León, Weng Herng Leong, Tracy How, Natalia Bazanova, Andrew Spriggs, Jean-Philippe Vielle-Calzada and Anna M. G. Koltunow

    Citation: Plant Methods 2020 16:88

    Content type: Methodology

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  8. Rapid non-destructive measurements to predict cassava root yield over the full growing season through large numbers of germplasm and multiple environments is a huge challenge in Cassava breeding programs. As o...

    Authors: Michael Gomez Selvaraj, Manuel Valderrama, Diego Guzman, Milton Valencia, Henry Ruiz and Animesh Acharjee

    Citation: Plant Methods 2020 16:87

    Content type: Research

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  9. Plant discrimination is of relevance for taxonomic, evolutionary, breeding and nutritional studies. To this purpose, evidence is reported to demonstrate TBP (Tubulin-Based-Polymorphism) as a DNA-based method s...

    Authors: Luca Braglia, Floriana Gavazzi, Laura Morello, Silvia Gianì, Peter Nick and Diego Breviario

    Citation: Plant Methods 2020 16:86

    Content type: Research

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  10. The number of studies using third-generation sequencing utilising Pacific Biosciences (PacBio) and Oxford Nanopore Technologies (ONT) is rapidly increasing in many different research areas. Among them, plant f...

    Authors: Jiawen Cui, Nan shen, Zhaogeng Lu, Guolu Xu, Yuyao Wang and Biao Jin

    Citation: Plant Methods 2020 16:85

    Content type: Methodology

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  11. Ingrowth-core method is a useful tool to determine fine root growth of standing crops by inserting root-free soil in mesh-bags for certain period of time. However, the root density observed by the method does ...

    Authors: Eusun Han, Dorte Bodin Dresbøll and Kristian Thorup-Kristensen

    Citation: Plant Methods 2020 16:84

    Content type: Methodology

    Published on:

  13. Many metals are essential for plants and humans. Knowledge of metal distribution in plant tissues in vivo contributes to the understanding of physiological mechanisms of metal uptake, accumulation and sequestr...

    Authors: Ana Mijovilovich, Filis Morina, Syed Nadeem Bokhari, Timo Wolff and Hendrik Küpper

    Citation: Plant Methods 2020 16:82

    Content type: Methodology

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  14. Generation of marker-free transgenic plants is very important to the regulatory permission and commercial release of transgenic crops. Co-transformation methods that enable the removal of selectable marker gen...

    Authors: Fang Liu, Pandi Wang, Xiaojuan Xiong, Ping Fu, Hongfei Gao, Xinhua Ding and Gang Wu

    Citation: Plant Methods 2020 16:81

    Content type: Research

    Published on:

  15. Obtaining high-quality RNA for gene expression analyses from different seed tissues is challenging due to the presence of various contaminants, such as polyphenols, polysaccharides and lipids which interfere w...

    Authors: Laura Siles, Peter Eastmond and Smita Kurup

    Citation: Plant Methods 2020 16:80

    Content type: Methodology

    Published on:

  16. The number of kernels per ear is one of the major agronomic yield indicators for maize. Manual assessment of kernel traits can be time consuming and laborious. Moreover, manually acquired data can be influence...

    Authors: Di Wu, Zhen Cai, Jiwan Han and Huawei Qin

    Citation: Plant Methods 2020 16:79

    Content type: Methodology

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  18. A fast, reliable and non-destructive method is needed to qualify the extractives content (EC) in heartwood of T. sinensis cores in the breeding program for studying the genetic effect on EC. However, the influenc...

    Authors: Yanjie Li, Xin Dong, Yang Sun, Jun Liu and Jingmin Jiang

    Citation: Plant Methods 2020 16:77

    Content type: Research

    Published on:

  19. Strigolactones (SLs) are important plant hormones that are produced via the carotenoid biosynthetic pathway and occur at extremely low concentrations in various plant species. They regulate root development, p...

    Authors: Rostislav Halouzka, Sanja Ćavar Zeljković, Bořivoj Klejdus and Petr Tarkowski

    Citation: Plant Methods 2020 16:76

    Content type: Review

    Published on:

  20. Glucosinolates are an important class of secondary metabolites characteristic to the order Brassicales. They are known to play a major role in plant defense and from the human perspective, can be anticarcinoge...

    Authors: Deepti M. Nambiar, Juhi Kumari, Gulab C. Arya, Amarjeet K. Singh and Naveen C. Bisht

    Citation: Plant Methods 2020 16:75

    Content type: Methodology

    Published on:

  21. Next generation sequencing (NGS) has been widely used in biological research, due to its rapid decrease in cost and increasing ability to generate data. However, while the sequence generation step has seen man...

    Authors: Sheng Zhao, Cuicui Zhang, Jianqiang Mu, Hui Zhang, Wen Yao, Xinhua Ding, Junqiang Ding and Yuxiao Chang

    Citation: Plant Methods 2020 16:74

    Content type: Methodology

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  23. There is little information on the effect of nutrient solutions composition on Arabidopsis growth. Therefore, we compared growth performance of Arabidopsis thaliana (Col-0) grown on the most commonly used nutrien...

    Authors: Sander H. van Delden, Mohammad Javad Nazarideljou and Leo F. M. Marcelis

    Citation: Plant Methods 2020 16:72

    Content type: Research

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  24. In situ analysis of biomarkers such as DNA, RNA and proteins are important for research and diagnostic purposes. At the RNA level, plant gene expression studies rely on qPCR, RNAseq and probe-based in situ hyb...

    Authors: Shyam Solanki, Gazala Ameen, Jin Zhao, Jordan Flaten, Pawel Borowicz and Robert S. Brueggeman

    Citation: Plant Methods 2020 16:71

    Content type: Methodology

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  25. Auxin response factors (ARFs) have long been a research focus and represent a class of key regulators of plant growth and development. Integrated phylogenomic synteny network analyses were able to provide nove...

    Authors: Bei Gao, Liuqiang Wang, Melvin Oliver, Moxian Chen and Jianhua Zhang

    Citation: Plant Methods 2020 16:70

    Content type: Research

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  26. Precision agriculture is an emerging research field that relies on monitoring and managing field variability in phenotypic traits. An important phenotypic trait is biomass, a comprehensive indicator that can r...

    Authors: Shichao Jin, Yanjun Su, Shilin Song, Kexin Xu, Tianyu Hu, Qiuli Yang, Fangfang Wu, Guangcai Xu, Qin Ma, Hongcan Guan, Shuxin Pang, Yumei Li and Qinghua Guo

    Citation: Plant Methods 2020 16:69

    Content type: Research

    Published on:

  27. RNAseq is nowadays the method of choice for transcriptome analysis. In the last decades, a high number of statistical methods, and associated bioinformatics tools, for RNAseq analysis were developed. More rece...

    Authors: Ilana Lambert, Christine Paysant-Le Roux, Stefano Colella and Marie-Laure Martin-Magniette

    Citation: Plant Methods 2020 16:68

    Content type: Software

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  28. Stalk lodging (breaking of agricultural plant stalks prior to harvest) results in millions of dollars in lost revenue each year. Despite a growing body of literature on the topic of stalk lodging, the structur...

    Authors: Christopher J. Stubbs, Kate Seegmiller, Christopher McMahan, Rajandeep S. Sekhon and Daniel J. Robertson

    Citation: Plant Methods 2020 16:67

    Content type: Research

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  29. X-ray computed tomography (CT) allows us to visualize root system architecture (RSA) beneath the soil, non-destructively and in a three-dimensional (3-D) form. However, CT scanning, reconstruction processes, a...

    Authors: Shota Teramoto, Satoko Takayasu, Yuka Kitomi, Yumiko Arai-Sanoh, Takanari Tanabata and Yusaku Uga

    Citation: Plant Methods 2020 16:66

    Content type: Methodology

    Published on:

  33. Field-grown leafy vegetables can be damaged by biotic and abiotic factors, or mechanically damaged by farming practices. Available methods to evaluate leaf tissue damage mainly rely on colour differentiation b...

    Authors: Emina Mulaosmanovic, Tobias U. T. Lindblom, Marie Bengtsson, Sofia T. Windstam, Lars Mogren, Salla Marttila, Hartmut Stützel and Beatrix W. Alsanius

    Citation: Plant Methods 2020 16:62

    Content type: Methodology

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  35. Plant parasitic weeds belonging to the genus Striga are a major threat for food production in Sub-Saharan Africa and Southeast Asia. The parasite’s life cycle starts with the induction of seed germination by host...

    Authors: Raul Masteling, Lodewijk Voorhoeve, Joris IJsselmuiden, Francisco Dini-Andreote, Wietse de Boer and Jos M. Raaijmakers

    Citation: Plant Methods 2020 16:60

    Content type: Software

    Published on:

  36. The increase in atmospheric CO2 is causing a number of changes in plant growth such as increases in leaf area and number, branching, plant size and biomass, and growth rate. Despite the importance of stomatal res...

    Authors: Mengmeng Tang, Xiaowei Zhao, Yinling Hu, Miaomiao Zeng, Kai Wang, Nannan Dong, Xiaonan Ma, Ling Bai and Chun-Peng Song

    Citation: Plant Methods 2020 16:59

    Content type: Methodology

    Published on:

  37. Quantitative PCR (qPCR) is one of the most common and accurate methods of gene expression analysis. However, the biggest challenge for this kind of examinations is normalization of the results, which requires ...

    Authors: Karolina Dudziak, Magdalena Sozoniuk, Hubert Szczerba, Adam Kuzdraliński, Krzysztof Kowalczyk, Andreas Börner and Michał Nowak

    Citation: Plant Methods 2020 16:58

    Content type: Research

    Published on:

  38. To study the impact of climate warming on crops, it is crucial to have a warming equipment suitable for their field environment. A facility is needed that can provide suitable combinations of different tempera...

    Authors: Zuolin Zhang, Zhiyuan Yang, Shah Fahad, Tong Zhang, Wenhao Xu, Kehui Cui, Shaobing Peng and Jianliang Huang

    Citation: Plant Methods 2020 16:57

    Content type: Research

    Published on:

  39. Wild apple, Malus sieversii, is an endangered species and a valuable genetic resource that requires a variety of conservation techniques. This study aimed to investigate the influence of different concentrations ...

    Authors: Y. Zhang, T. A. Bozorov, D. X. Li, P. Zhou, X. J. Wen, Y. Ding and D. Y. Zhang

    Citation: Plant Methods 2020 16:56

    Content type: Research

    Published on:

  40. To ensure further genetic gain, genomic approaches in plant breeding rely on precise phenotypic data, describing plant structure, function and performance. A more precise characterization of the environment wi...

    Authors: Andreas Honecker, Henrik Schumann, Diana Becirevic, Lasse Klingbeil, Kai Volland, Steffi Forberig, Marc Jansen, Hinrich Paulsen, Heiner Kuhlmann and Jens Léon

    Citation: Plant Methods 2020 16:55

    Content type: Software

    Published on:

  41. Plant hormones are naturally occurring small molecule compounds which are present at trace amounts in plant. They play a pivotal role in the regulation of plant growth. The biological activity of plant hormone...

    Authors: Liyuan Wang, Yilin Zou, Han Yeong Kaw, Gang Wang, Huaze Sun, Long Cai, Chengyu Li, Long-Yue Meng and Donghao Li

    Citation: Plant Methods 2020 16:54

    Content type: Review

    Published on:

  42. Phytoplasma are obligate intracellular plant-pathogenic bacteria that infect a broad range of plant species and are transmitted by different insect species. Quantitative real-time PCR (qPCR) is one of the most...

    Authors: Cecilia Mittelberger, Lisa Obkircher, Vicky Oberkofler, Alan Ianeselli, Christine Kerschbamer, Andreas Gallmetzer, Yazmid Reyes-Dominguez, Thomas Letschka and Katrin Janik

    Citation: Plant Methods 2020 16:53

    Content type: Methodology

    Published on:

  43. Functional genomic studies using genetics approaches of conifers are hampered by the complex and enormous genome, long vegetative growth period, and exertion in genetic transformation. Thus, the research carri...

    Authors: Shuangwei Liu, Jingjing Ma, Hongmei Liu, Yingtian Guo, Wei Li and Shihui Niu

    Citation: Plant Methods 2020 16:52

    Content type: Methodology

    Published on:

  44. Rain-induced fruit cracking is a major physiological problem in most sweet cherry cultivars. For an in vivo cracking assay, the ‘Christensen method’ (cracking evaluation following fruit immersion in water) is ...

    Authors: Michail Michailidis, Evangelos Karagiannis, Georgia Tanou, Eirini Sarrou, Katerina Karamanoli, Athina Lazaridou, Stefan Martens and Athanassios Molassiotis

    Citation: Plant Methods 2020 16:51

    Content type: Research

    Published on:

  45. The genus Utricularia belongs to Lentibulariaceae, the largest family of carnivorous plants, which includes terrestrial, epiphytic and aquatic species. The development of specialized structures that evolved for c...

    Authors: A. Oropeza-Aburto, S. A. Cervantes-Pérez, V. A. Albert and L. Herrera-Estrella

    Citation: Plant Methods 2020 16:50

    Content type: Methodology

    Published on:

  46. Quantitative and qualitative assessment of visual and morphological traits of seed is slow and imprecise with potential for bias to be introduced when gathered with handheld tools. Colour, size and shape trait...

    Authors: Keith Halcro, Kaitlin McNabb, Ashley Lockinger, Didier Socquet-Juglard, Kirstin E. Bett and Scott D. Noble

    Citation: Plant Methods 2020 16:49

    Content type: Methodology

    Published on:

  47. Many methodological approaches have focused so far on physiological and molecular responses of plant tissues to freezing but only little knowledge is available on the consequences of extracellular ice-formatio...

    Authors: Othmar Buchner, Philip Steiner, Ancuela Andosch, Andreas Holzinger, Matthias Stegner, Gilbert Neuner and Ursula Lütz-Meindl

    Citation: Plant Methods 2020 16:48

    Content type: Methodology

    Published on:

  48. Crocus sativus is a recalcitrant plant for genetic transformation and genetic improvement, largely due to difficulties in Agrobacterium mediated transformation and vegetative reproduction. Effective genome editin...

    Authors: Sudha Chib, Arulprakash Thangaraj, Sanjana Kaul, Manoj Kumar Dhar and Tanushri Kaul

    Citation: Plant Methods 2020 16:47

    Content type: Research

    Published on:

  49. Stable isotopically labelled organisms have found wide application in life science research including plant physiology, plant stress and defense as well as metabolism related sciences. Therefore, the reproduci...

    Authors: Asja Ćeranić, Maria Doppler, Christoph Büschl, Alexandra Parich, Kangkang Xu, Andrea Koutnik, Hermann Bürstmayr, Marc Lemmens and Rainer Schuhmacher

    Citation: Plant Methods 2020 16:46

    Content type: Methodology

    Published on:

  50. Short read sequencing technologies, such as genotyping-by-sequencing (GBS), have been utilized in genetic mapping, marker development, and population genomic studies. High-throughput and multiplexing capabilit...

    Authors: Narinder Singh, John Raupp, Dal-Hoe Koo, Bernd Friebe, Bikram Gill and Jesse Poland

    Citation: Plant Methods 2020 16:45

    Content type: Methodology

    Published on:

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ISSN: 1746-4811