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  1. Leaf hairiness (pubescence) is an important plant phenotype which regulates leaf transpiration, affects sunlight penetration, and provides increased resistance or susceptibility against certain insects. Cotton...

    Authors: Vivien Rolland, Moshiur R. Farazi, Warren C. Conaty, Deon Cameron, Shiming Liu, Lars Petersson and Warwick N. Stiller

    Citation: Plant Methods 2022 18:8

    Content type: Methodology

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  2. Ralstonia solanacearum, one of the most devastating bacterial plant pathogens, is the causal agent of bacterial wilt. Recently, several studies on resistance to bacterial wilt have been conducted using the Arabid...

    Authors: Cuihong Xu, Lingkun Zhong, Zeming Huang, Chenying Li, Jiazhang Lian, Xuefang Zheng and Yan Liang

    Citation: Plant Methods 2022 18:7

    Content type: Methodology

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  3. The incorporation of root traits into elite germplasm is typically a slow process. Thus, innovative approaches are required to accelerate research and pre-breeding programs targeting root traits to improve yie...

    Authors: Charlotte Rambla, Sarah Van Der Meer, Kai P. Voss-Fels, Manar Makhoul, Christian Obermeier, Rod Snowdon, Eric S. Ober, Michelle Watt, Samir Alahmad and Lee T. Hickey

    Citation: Plant Methods 2022 18:2

    Content type: Methodology

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  4. Lupins are promising protein crops with an increasing amount of genomic and transcriptomic resources. The new resources facilitate the in silico identification of candidate genes controlling important agronomi...

    Authors: Davide Mancinotti, Maria Cecilia Rodriguez, Karen Michiko Frick, Bjørn Dueholm, Ditte Goldschmidt Jepsen, Niels Agerbirk and Fernando Geu-Flores

    Citation: Plant Methods 2021 17:131

    Content type: Research

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  5. With increasing joint research cooperation on national and international levels, there is a high need for harmonized and reproducible cultivation conditions and experimental protocols in order to ensure the be...

    Authors: Paul Grünhofer, Yayu Guo, Ruili Li, Jinxing Lin and Lukas Schreiber

    Citation: Plant Methods 2021 17:129

    Content type: Methodology

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  6. The leaf epidermis functions to prevent the loss of water and reduce gas exchange. As an interface between the plant and its external environment, it helps prevent damage, making it an attractive system for st...

    Authors: Jieping Li, Xinlei Feng and Jinjin Xie

    Citation: Plant Methods 2021 17:128

    Content type: Methodology

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  7. 3D imaging, such as X-ray CT and MRI, has been widely deployed to study plant root structures. Many computational tools exist to extract coarse-grained features from 3D root images, such as total volume, root ...

    Authors: Dan Zeng, Mao Li, Ni Jiang, Yiwen Ju, Hannah Schreiber, Erin Chambers, David Letscher, Tao Ju and Christopher N. Topp

    Citation: Plant Methods 2021 17:127

    Content type: Methodology

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  8. The bacterial leaf pathogen Pseudomonas syringae pv tomato (Pst) is the most popular model pathogen for plant pathology research. Previous methods to study the plant-Pst interactions rely on destructive quantific...

    Authors: Leonardo Furci, David Pascual-Pardo and Jurriaan Ton

    Citation: Plant Methods 2021 17:126

    Content type: Methodology

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  9. The root system architecture (RSA) of alfalfa (Medicago sativa L.) affects biomass production by influencing water and nutrient uptake, including nitrogen fixation. Further, roots are important for storing carboh...

    Authors: Bruna Bucciarelli, Zhanyou Xu, Samadangla Ao, Yuanyuan Cao, Maria J. Monteros, Christopher N. Topp and Deborah A. Samac

    Citation: Plant Methods 2021 17:125

    Content type: Research

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  10. Ribo-seq has revolutionized the study of genome-wide mRNA translation. High-quality Ribo-seq data display strong 3-nucleotide (nt) periodicity, which corresponds to translating ribosomes deciphering three nts ...

    Authors: Hsin-Yen Larry Wu and Polly Yingshan Hsu

    Citation: Plant Methods 2021 17:124

    Content type: Software

    Published on:

  11. Breakthrough imaging technologies may challenge the plant phenotyping bottleneck regarding marker-assisted breeding and genetic mapping. In this context, X-Ray CT (computed tomography) technology can accuratel...

    Authors: Monica Herrero-Huerta, Valerian Meline, Anjali S. Iyer-Pascuzzi, Augusto M. Souza, Mitchell R. Tuinstra and Yang Yang

    Citation: Plant Methods 2021 17:123

    Content type: Research

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  12. Blackleg disease, caused by the fungal pathogen Leptosphaeria maculans, is a serious threat to canola (Brassica napus) production worldwide. Quantitative resistance to this disease is a highly desirable trait but...

    Authors: Wendelin Schnippenkoetter, Mohammad Hoque, Rebecca Maher, Angela Van de Wouw, Phillip Hands, Vivien Rolland, Luke Barrett and Susan Sprague

    Citation: Plant Methods 2021 17:122

    Content type: Methodology

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  13. The rice gall midge (RGM, Orseolia oryzae, Wood-Mason), an important stem-feeding pest worldwide, has caused serious production losses over the past decades. Rice production practices indicate that the most relia...

    Authors: Ling Cheng, Fugang Huang, Zhe Jiang, Baiyi Lu, Xiaohui Zhong and Yongfu Qiu

    Citation: Plant Methods 2021 17:121

    Content type: Research

    Published on:

  14. Low temperature freezing stress has adverse effects on wheat seedling growth and final yield. The traditional method to evaluate the wheat injury caused by the freezing stress is by visual observations, which ...

    Authors: Xiuqing Fu, Yang Bai, Jing Zhou, Hongwen Zhang and Jieyu Xian

    Citation: Plant Methods 2021 17:120

    Content type: Research

    Published on:

  15. Due to the high cost of data collection for magnetization detection of media, the sample size is limited, it is not suitable to use deep learning method to predict its change trend. The prediction of physical ...

    Authors: Jing Nie, Nianyi Wang, Jingbin Li, Kang Wang and Hongkun Wang

    Citation: Plant Methods 2021 17:119

    Content type: Research

    Published on:

  16. Realizing imaging detection of water and nitrogen content in different regions of plant leaves in-site and real-time can provide an efficient new technology for determining crop drought resistance and nutrient...

    Authors: Ning Zhang, Peng-cheng Li, Hubin Liu, Tian-cheng Huang, Han Liu, Yu Kong, Zhi-cheng Dong, Yu-hui Yuan, Long-lian Zhao and Jun-hui Li

    Citation: Plant Methods 2021 17:117

    Content type: Research

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  17. Macrophytes are key players in aquatic ecosystems diversity, but knowledge on variability of their functional traits, among and within species, is still limited. Remote sensing is a high-throughput, feasible o...

    Authors: Paolo Villa, Rossano Bolpagni, Monica Pinardi and Viktor R. Tóth

    Citation: Plant Methods 2021 17:115

    Content type: Methodology

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  18. Multiplex CRISPR-Cas9-based genome editing is an efficient method for targeted disruption of gene function in plants. Use of CRISPR-Cas9 has increased rapidly in recent years and is becoming a routine method f...

    Authors: Robertas Ursache, Satoshi Fujita, Valérie Dénervaud Tendon and Niko Geldner

    Citation: Plant Methods 2021 17:111

    Content type: Methodology

    Published on:

  19. Grapevine, as an essential fruit crop with high economic values, has been the focus of molecular studies in diverse areas. Two challenges exist in the grapevine research field: (i) the lack of a rapid, user-fr...

    Authors: Yashu Song, Robert H. Hanner and Baozhong Meng

    Citation: Plant Methods 2021 17:110

    Content type: Research

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  20. Cellular components are controlled by genetic and physiological factors that define their shape and size. However, quantitively capturing the morphological characteristics and movement of cellular organelles f...

    Authors: Arpan Kumar Basak, Mohamadreza Mirzaei, Kazimierz Strzałka and Kenji Yamada

    Citation: Plant Methods 2021 17:109

    Content type: Research

    Published on:

  21. The need for rapid in-field measurement of key traits contributing to yield over many thousands of genotypes is a major roadblock in crop breeding. Recently, leaf hyperspectral reflectance data has been used t...

    Authors: Robert T. Furbank, Viridiana Silva-Perez, John R. Evans, Anthony G. Condon, Gonzalo M. Estavillo, Wennan He, Saul Newman, Richard Poiré, Ashley Hall and Zhen He

    Citation: Plant Methods 2021 17:108

    Content type: Methodology

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  22. The characteristics of light source have an important influence on the measurement performance of canopy reflectance spectrometer. The size of the effective irradiation area and the uniformity of the light int...

    Authors: Hongfeng Yu, Yongqian Ding, Huanliang Xu, Xueni Wu and Xianglin Dou

    Citation: Plant Methods 2021 17:107

    Content type: Research

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  23. The Agrobacterium-mediated transient transformation, which proved effective in diverse plant species, has been widely applied for high-throughput gene function studies due to its simplicity, rapidity, and high ef...

    Authors: Yongpeng Li, Tiantian Chen, Wei Wang, Hang Liu, Xin Yan, Kuanyu Wu-Zhang, Wei Qin, Lihui Xie, Yaojie Zhang, Bowen Peng, Xinghao Yao, Chen Wang, Sadaf-Ilyas Kayani, Xueqing Fu, Ling Li and Kexuan Tang

    Citation: Plant Methods 2021 17:106

    Content type: Methodology

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  24. Zinc (Zn) is an important nutrient for human beings, which is also an essential micronutrient for crop growth. This study investigated the role of Zn in coordinating the mineral elements absorption in modern w...

    Authors: Xiaolong Guo, Xiangyu Ma, Jialiang Zhang, Jinghuan Zhu, Tian Lu, Qifei Wang, Xiaoming Wang, Wei Hua and Shengbao Xu

    Citation: Plant Methods 2021 17:105

    Content type: Research

    Published on:

  25. Litchi chinensis Sonn. is an economically important fruit tree in tropical and subtropical regions. However, litchi functional genomics is severely hindered due to its recalcitrance to regeneration and stable tra...

    Authors: Yaqi Qin, Dan Wang, Jiaxin Fu, Zhike Zhang, Yonghua Qin, Guibing Hu and Jietang Zhao

    Citation: Plant Methods 2021 17:103

    Content type: Research

    Published on:

  26. Since the beginning of the use of reporter proteins for expression analysis, a variety of approaches have been developed and proposed; both qualitative and quantitative. The lack of simple methods for direct o...

    Authors: Alexander A. Tyurin, Aleksandra V. Suhorukova, Igor V. Deineko, Olga S. Pavlenko, Viktoriia A. Fridman and Irina V. Goldenkova-Pavlova

    Citation: Plant Methods 2021 17:102

    Content type: Methodology

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  27. Flexural three-point bending tests are useful for characterizing the mechanical properties of plant stems. These tests can be performed with minimal sample preparation, thus allowing tests to be performed rela...

    Authors: Nathanael Martin-Nelson, Brandon Sutherland, Michael Yancey, Chung Shan Liao, Christopher J. Stubbs and Douglas D. Cook

    Citation: Plant Methods 2021 17:101

    Content type: Methodology

    Published on:

  28. Transgenic plant suspension cells show economic potential for the production of valuable bioproducts. The sugar starvation-inducible rice αAmy3 promoter, together with its signal peptide, is widely applied to pro...

    Authors: Desyanti Saulina Sinaga, Shin-Lon Ho, Chung-An Lu, Su-May Yu and Li-Fen Huang

    Citation: Plant Methods 2021 17:99

    Content type: Research

    Published on:

  29. Apricot is cultivated worldwide because of its high nutritive content and strong adaptability. Its flesh is delicious and has a unique and pleasant aroma. Apricot kernel is also consumed as nuts. The genome of...

    Authors: Chen Chen, Huimin Liu, Ningning Gou, Mengzhen Huang, Wanyu Xu, Xuchun Zhu, Mingyu Yin, Haikun Bai, Lin Wang and Ta-na Wuyun

    Citation: Plant Methods 2021 17:98

    Content type: Database

    Published on:

  30. Detailed datasets containing root system and its architecture in soil are required to improve understanding of resource capture by roots. However, most of the root study methods have paid little attention to m...

    Authors: Takuya Koyama, Shun Murakami, Toshihiko Karasawa, Masato Ejiri and Katsuhiro Shiono

    Citation: Plant Methods 2021 17:97

    Content type: Methodology

    Published on:

  31. Fractional vegetation cover (FVC) is an important basic parameter for the quantitative monitoring of the alpine grassland ecosystem on the Qinghai-Tibetan Plateau. Based on unmanned aerial vehicle (UAV) acquis...

    Authors: Xingchen Lin, Jianjun Chen, Peiqing Lou, Shuhua Yi, Yu Qin, Haotian You and Xiaowen Han

    Citation: Plant Methods 2021 17:96

    Content type: Research

    Published on:

  32. Ploidy manipulation is effective in seedless loquat breeding, in which flesh color is a key agronomic and economic trait. Few techniques are currently available for detecting the genotypes of polyploids in pla...

    Authors: Haiyan Wang, Jiangbo Dang, Di Wu, Zhongyi Xie, Shuang Yan, Jingnan Luo, Qigao Guo and Guolu Liang

    Citation: Plant Methods 2021 17:93

    Content type: Methodology

    Published on:

  33. Translation is a tightly regulated process, controlling the rate of protein synthesis in cells. Ribosome sequencing (Ribo-Seq) is a recently developed tool for studying actively translated mRNA and can thus di...

    Authors: Amir Mahboubi, Nicolas Delhomme, Sara Häggström and Johannes Hanson

    Citation: Plant Methods 2021 17:92

    Content type: Methodology

    Published on:

  34. Maize cobs are an important component of crop yield that exhibit a high diversity in size, shape and color in native landraces and modern varieties. Various phenotyping approaches were developed to measure mai...

    Authors: Lydia Kienbaum, Miguel Correa Abondano, Raul Blas and Karl Schmid

    Citation: Plant Methods 2021 17:91

    Content type: Methodology

    Published on:

  35. Estimation of nitrate nitrogen (NO3–N) content in petioles is one of the key approaches for monitoring nitrogen (N) nutrition in crops. Rapid, non-destructive, and accurate evaluation of NO3–N contents in cotto...

    Authors: Zhiqiang Dong, Yang Liu, Baoxia Ci, Ming Wen, Minghua Li, Xi Lu, Xiaokang Feng, Shuai Wen and Fuyu Ma

    Citation: Plant Methods 2021 17:90

    Content type: Methodology

    Published on:

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