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Plant Methods

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  1. Content type: Research

    Leaf chlorophyll content (LCC) provides valuable information about plant physiology. Most of the published chlorophyll vegetation indices at the leaf level have been based on the spectral characteristics of th...

    Authors: Shan Lu, Fan Lu, Wenqiang You, Zheyi Wang, Yu Liu and Kenji Omasa

    Citation: Plant Methods 2018 14:15

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  2. Content type: Methodology

    Lipidomics plays an important role in understanding plant adaptation to different stresses and improving our knowledge of the genes underlying lipid metabolism. Lipidomics involves lipid extraction, sample pre...

    Authors: Sunitha Shiva, Regina Enninful, Mary R. Roth, Pamela Tamura, Krishna Jagadish and Ruth Welti

    Citation: Plant Methods 2018 14:14

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  3. Content type: Research

    Stalk lodging is a serious challenge in the production of maize and sorghum. A comprehensive understanding of lodging will likely require accurate characterizations of the mechanical properties of such plants....

    Authors: Loay Al-Zube, Wenhuan Sun, Daniel Robertson and Douglas Cook

    Citation: Plant Methods 2018 14:11

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  4. Content type: Methodology

    Genome-wide characterization of tissue- or cell-specific gene expression is a recurrent bottleneck in biology. We have developed a sensitive approach based on ultra-low RNA sequencing coupled to laser assisted...

    Authors: Kaori Sakai, Ludivine Taconnat, Nero Borrega, Jennifer Yansouni, Véronique Brunaud, Christine Paysant-Le Roux, Etienne Delannoy, Marie-Laure Martin Magniette, Loïc Lepiniec, Jean Denis Faure, Sandrine Balzergue and Bertrand Dubreucq

    Citation: Plant Methods 2018 14:10

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  5. Content type: Methodology

    Due to the nature of viral RNA genomes, RNA viruses depend on many RNA-binding proteins (RBP) of viral and host origin for replication, dissemination and evasion of host RNA degradation pathways. Some viruses ...

    Authors: F. E. Marmisolle, M. L. García and C. A. Reyes

    Citation: Plant Methods 2018 14:9

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  6. Content type: Methodology

    Leaf venation traits are important for many research fields such as systematics and evolutionary biology, plant physiology, climate change, and paleoecology. In spite of an increasing demand for vein trait dat...

    Authors: Julio V. Schneider, Renate Rabenstein, Jens Wesenberg, Karsten Wesche, Georg Zizka and Jörg Habersetzer

    Citation: Plant Methods 2018 14:7

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  7. Content type: Methodology

    The amino acid profile of plants is an important parameter in assessments of their growth potential, resource-use efficiency and/or quality as food and feed. Screening studies may involve large number of sampl...

    Authors: Rasmus Dahl-Lassen, Jan van Hecke, Henning Jørgensen, Christian Bukh, Birgit Andersen and Jan K. Schjoerring

    Citation: Plant Methods 2018 14:8

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  8. Content type: Methodology

    Deep learning presents many opportunities for image-based plant phenotyping. Here we consider the capability of deep convolutional neural networks to perform the leaf counting task. Deep learning techniques ty...

    Authors: Jordan Ubbens, Mikolaj Cieslak, Przemyslaw Prusinkiewicz and Ian Stavness

    Citation: Plant Methods 2018 14:6

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  9. Content type: Methodology

    Miscanthus is a leading second generation bio-energy crop. It is mostly rhizome propagated; however, the increasing use of seed is resulting in a greater need to investigate germination. Miscanthus seed are small...

    Authors: Danny Awty-Carroll, John Clifton-Brown and Paul Robson

    Citation: Plant Methods 2018 14:5

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  10. Content type: Methodology

    Transient gene expression is a powerful tool to study gene function in plants. In citrus, Agrobacterium transformation is the method of choice for transient expression studies, but this method does not work effic...

    Authors: Amit Levy, Choaa El-Mochtar, Chunxia Wang, Michael Goodin and Vladimir Orbovic

    Citation: Plant Methods 2018 14:2

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  11. Content type: Methodology

    Low pollen viability may limit grapevine yield under certain conditions, causing relevant economic losses to grape-growers. It is usually evaluated by the quantification of the number of viable and non-viable ...

    Authors: Javier Tello, María Ignacia Montemayor, Astrid Forneck and Javier Ibáñez

    Citation: Plant Methods 2018 14:3

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  12. Content type: Software

    Plants demonstrate dynamic growth phenotypes that are determined by genetic and environmental factors. Phenotypic analysis of growth features over time is a key approach to understand how plants interact with ...

    Authors: Ji Zhou, Christopher Applegate, Albor Dobon Alonso, Daniel Reynolds, Simon Orford, Michal Mackiewicz, Simon Griffiths, Steven Penfield and Nick Pullen

    Citation: Plant Methods 2017 13:117

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  13. Content type: Methodology

    Fine-grained recognition of plants from images is a challenging computer vision task, due to the diverse appearance and complex structure of plants, high intra-class variability and small inter-class differenc...

    Authors: Milan Šulc and Jiří Matas

    Citation: Plant Methods 2017 13:115

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  14. Content type: Software

    Developmental biology has made great strides in recent years towards the quantification of cellular properties during development. This requires tissues to be imaged and segmented to generate computerised vers...

    Authors: Annamária Kiss, Typhaine Moreau, Vincent Mirabet, Cerasela Iliana Calugaru, Arezki Boudaoud and Pradeep Das

    Citation: Plant Methods 2017 13:114

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  15. Content type: Methodology

    Switchgrass (Panicum virgatum), a robust perennial C4-type grass, has been evaluated and designated as a model bioenergy crop by the U.S. DOE and USDA. Conventional breeding of switchgrass biomass is difficult be...

    Authors: Chien-Yuan Lin, Bryon S. Donohoe, Neha Ahuja, Deborah M. Garrity, Rongda Qu, Melvin P. Tucker, Michael E. Himmel and Hui Wei

    Citation: Plant Methods 2017 13:113

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  16. Content type: Methodology

    To investigate translational regulation of gene expression in plant mitochondria, a mitochondrial polysome isolation protocol was established for tobacco to investigate polysomal mRNA loading as a proxy for tr...

    Authors: Muhammad Waqar Hameed, Ilona Juszczak, Ralph Bock and Joost Thomas van Dongen

    Citation: Plant Methods 2017 13:112

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  17. Content type: Research

    The development of cell pattern in the surface cell layer of the shoot apex can be investigated in vivo by use of a time-lapse confocal images, showing naked meristem in 3D in successive times. However, how th...

    Authors: Krzysztof Kucypera, Marcin Lipowczan, Anna Piekarska-Stachowiak and Jerzy Nakielski

    Citation: Plant Methods 2017 13:110

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  18. Content type: Methodology

    The morphological analysis of olive leaves, fruits and endocarps may represent an efficient tool for the characterization and discrimination of cultivars and the establishment of relationships among them. In r...

    Authors: Konstantinos N. Blazakis, Maria Kosma, George Kostelenos, Luciana Baldoni, Marina Bufacchi and Panagiotis Kalaitzis

    Citation: Plant Methods 2017 13:111

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  19. Content type: Methodology

    Sorghum (Sorghum bicolor L.) is one of the world’s most important cereal crops grown for multiple applications and has been identified as a potential biofuel crop. Despite several decades of study, sorghum has be...

    Authors: Srinivas Belide, Thomas Vanhercke, James Robertson Petrie and Surinder Pal Singh

    Citation: Plant Methods 2017 13:109

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  20. Content type: Methodology

    The proportion of granule types in wheat starch is an important characteristic that can affect its functionality. It is widely accepted that granule types are either large, disc-shaped A-type granules or small...

    Authors: Emi Tanaka, Jean-Phillippe F. Ral, Sean Li, Raj Gaire, Colin R. Cavanagh, Brian R. Cullis and Alex Whan

    Citation: Plant Methods 2017 13:107

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