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  1. Content type: Methodology

    The next-generation sequencing (NGS) technology has greatly facilitated genomic and transcriptomic studies, contributing significantly in expanding the current knowledge on genome and transcriptome. However, t...

    Authors: Fu-Yuan Zhu, Mo-Xian Chen, Neng-Hui Ye, Wang-Min Qiao, Bei Gao, Wai-Ki Law, Yuan Tian, Dong Zhang, Di Zhang, Tie-Yuan Liu, Qi-Juan Hu, Yun-Ying Cao, Ze-Zhuo Su, Jianhua Zhang and Ying-Gao Liu

    Citation: Plant Methods 2018 14:69

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  2. Content type: Methodology

    Full scent profiles emitted by living tissues can be screened by using total ion chromatograms generated in full scan mode and gas chromatography–mass spectrometry technique using Headspace Sorptive Extraction...

    Authors: Victoria Ruiz-Hernández, María José Roca, Marcos Egea-Cortines and Julia Weiss

    Citation: Plant Methods 2018 14:67

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  3. Content type: Methodology

    High resolution and high throughput genotype to phenotype studies in plants are underway to accelerate breeding of climate ready crops. In the recent years, deep learning techniques and in particular Convoluti...

    Authors: Sarah Taghavi Namin, Mohammad Esmaeilzadeh, Mohammad Najafi, Tim B. Brown and Justin O. Borevitz

    Citation: Plant Methods 2018 14:66

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  4. Content type: Research

    Our knowledge of natural genetic variation is increasing at an extremely rapid pace, affording an opportunity to come to a much richer understanding of how effects of specific genes are dependent on the geneti...

    Authors: Rui Wu, Miriam Lucke, Yun-ting Jang, Wangsheng Zhu, Efthymia Symeonidi, Congmao Wang, Joffrey Fitz, Wanyan Xi, Rebecca Schwab and Detlef Weigel

    Citation: Plant Methods 2018 14:65

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  5. Content type: Research

    The images of different flower species had small inter-class variations across different classes as well as large intra-class variations within a class. Flower classification techniques are mainly based on the...

    Authors: Ping Lin, Du Li, Zhiyong Zou, Yongming Chen and Shanchao Jiang

    Citation: Plant Methods 2018 14:64

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  6. Content type: Methodology

    The model species Arabidopsis thaliana has extensive resources to investigate intraspecific trait variability and the genetic bases of ecologically relevant traits. However, the cost of equipment and software req...

    Authors: François Vasseur, Justine Bresson, George Wang, Rebecca Schwab and Detlef Weigel

    Citation: Plant Methods 2018 14:63

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  7. Content type: Methodology

    Metabolomics based on mass spectrometry analysis are increasingly applied in diverse scientific domains, notably agronomy and plant biology, in order to understand plants’ behaviors under different stress cond...

    Authors: Hikmat Ghosson, Adrián Schwarzenberg, Frank Jamois and Jean-Claude Yvin

    Citation: Plant Methods 2018 14:62

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  8. Content type: Methodology

    Rhizobium rhizogenes transformation is commonly used to generate transgenic roots traditionally called hairy roots, for both investigative and commercial applications. While fertile plants can be regenerated from...

    Authors: Pradeepa C. G. Bandaranayake and John I. Yoder

    Citation: Plant Methods 2018 14:61

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  9. Content type: Methodology

    Matrix-assisted laser-desorption and ionisation time-of-flight mass spectroscopy (MALDI-TOF MS) is a powerful tool for the characterisation and/or identification of protein-containing samples. Several MALDI-TO...

    Authors: Michael A. Reeve, Kathryn M. Pollard and Daisuke Kurose

    Citation: Plant Methods 2018 14:60

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  10. Content type: Methodology

    In bioenergy/forage sorghum, morpho-anatomical stem properties are major components affecting standability and juice yield. However, phenotyping these traits is low-throughput, and has been restricted by the l...

    Authors: Francisco E. Gomez, Geraldo Carvalho Jr., Fuhao Shi, Anastasia H. Muliana and William L. Rooney

    Citation: Plant Methods 2018 14:59

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  11. Content type: Correction

    Unfortunately, in the original version [1] of this article, a funder note was missed out in the acknowledgement.

    Authors: Abelardo Montesinos-López, Osval A. Montesinos-López, Gustavo de los Campos, José Crossa, Juan Burgueño and Francisco Javier Luna-Vazquez

    Citation: Plant Methods 2018 14:57

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    The original article was published in Plant Methods 2018 14:46

  12. Content type: Methodology

    Small GTPases act as molecular switches that regulate various plant responses such as disease resistance, pollen tube growth, root hair development, cell wall patterning and hormone responses. Thus, to monito...

    Authors: Hann Ling Wong, Akira Akamatsu, Qiong Wang, Masayuki Higuchi, Tomonori Matsuda, Jun Okuda, Ken-ichi Kosami, Noriko Inada, Tsutomu Kawasaki, Takako Kaneko-Kawano, Shingo Nagawa, Li Tan, Yoji Kawano and Ko Shimamoto

    Citation: Plant Methods 2018 14:56

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  13. Content type: Research

    In studies of plant stress signaling, a major challenge is the lack of non-invasive methods to detect physiological plant responses and to characterize plant–plant communication over time and space.

    Authors: Leandro do Prado Ribeiro, Adriana Lídia Santana Klock, João Américo Wordell Filho, Marco Aurélio Tramontin, Marília Almeida Trapp, Axel Mithöfer and Christian Nansen

    Citation: Plant Methods 2018 14:54

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  14. Content type: Methodology

    Chickpea (Cicer arietinum L.), an important legume crop is one of the major source of dietary protein. Developing an efficient and reproducible transformation method is imperative to expedite functional genomics...

    Authors: Pooja Rani Aggarwal, Papri Nag, Pooja Choudhary, Niranjan Chakraborty and Subhra Chakraborty

    Citation: Plant Methods 2018 14:55

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  15. Content type: Research

    Plant height is an important morphological and developmental phenotype that directly indicates overall plant growth and is widely predictive of final grain yield and biomass. Currently, manually measuring plan...

    Authors: Xu Wang, Daljit Singh, Sandeep Marla, Geoffrey Morris and Jesse Poland

    Citation: Plant Methods 2018 14:53

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  16. Content type: Research

    Plant cell walls are nanocomposites based on cellulose microfibrils embedded in a matrix of polysaccharides and aromatic polymers. They are optimized for different functions (e.g. mechanical stability) by chan...

    Authors: Batirtze Prats-Mateu, Martin Felhofer, Anna de Juan and Notburga Gierlinger

    Citation: Plant Methods 2018 14:52

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  17. Content type: Research

    Metabolic fluxes represent the functional phenotypes of biochemical pathways and are essential to reveal the distribution of precursors among metabolic networks. Although analysis of metabolic fluxes, facilit...

    Authors: Peng Wang, Longyun Guo, Rohit Jaini, Antje Klempien, Rachel M. McCoy, John A. Morgan, Natalia Dudareva and Clint Chapple

    Citation: Plant Methods 2018 14:51

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  18. Content type: Methodology

    Genetically modified cotton accounts for 64% of the world’s cotton growing area (22.3 million hectares). The genome sequencing of the diploid cotton progenitors Gossypium raimondii and Gossypium arboreum as well ...

    Authors: Haipeng Li, Kun Li, Yutao Guo, Jinggong Guo, Kaiting Miao, Jose R. Botella, Chun-Peng Song and Yuchen Miao

    Citation: Plant Methods 2018 14:50

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  19. Content type: Methodology

    Grain yield, ear and kernel attributes can assist to understand the performance of maize plant under different environmental conditions and can be used in the variety development process to address farmer’s p...

    Authors: R. Makanza, M. Zaman-Allah, J. E. Cairns, J. Eyre, J. Burgueño, Ángela Pacheco, C. Diepenbrock, C. Magorokosho, A. Tarekegne, M. Olsen and B. M. Prasanna

    Citation: Plant Methods 2018 14:49

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  20. Content type: Methodology

    Hydroponic systems are a convenient platform for plant cultivation when treatments are applied to the roots because they provide precise control of the composition of the growth medium, ensuring the availabili...

    Authors: Miriam Gil-Monreal, Manuel Fernandez-Escalada, Mercedes Royuela and Ana Zabalza

    Citation: Plant Methods 2018 14:48

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  21. Content type: Research

    Preservation of plant virus is a fundamental requirement in all types of virus-related research and applied applications. Development of efficient, reliable strategies for long-term preservation of plant virus...

    Authors: Min-Rui Wang, Wen Yang, Lei Zhao, Jing-Wei Li, Ke Liu, Jing-Wei Yu, Yun-Feng Wu and Qiao-Chun Wang

    Citation: Plant Methods 2018 14:47

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  22. Content type: Methodology

    Modern agriculture uses hyperspectral cameras with hundreds of reflectance data at discrete narrow bands measured in several environments. Recently, Montesinos-López et al. (Plant Methods 13(4):1–23, 2017a.

    Authors: Abelardo Montesinos-López, Osval A. Montesinos-López, Gustavo de los Campos, José Crossa, Juan Burgueño and Francisco Javier Luna-Vazquez

    Citation: Plant Methods 2018 14:46

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    The Correction to this article has been published in Plant Methods 2018 14:57

  23. Content type: Research

    Phenotyping is a bottleneck for the development of new plant cultivars. This study introduces a new hyperspectral phenotyping system, which combines the high throughput of canopy scale measurements with the ad...

    Authors: Stefan Thomas, Jan Behmann, Angelina Steier, Thorsten Kraska, Onno Muller, Uwe Rascher and Anne-Katrin Mahlein

    Citation: Plant Methods 2018 14:45

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  24. Content type: Methodology

    The world’s herbaria contain millions of specimens, collected and named by thousands of researchers, over hundreds of years. However, this treasure has remained largely inaccessible to genetic studies, because...

    Authors: Chun-Xia Zeng, Peter M. Hollingsworth, Jing Yang, Zheng-Shan He, Zhi-Rong Zhang, De-Zhu Li and Jun-Bo Yang

    Citation: Plant Methods 2018 14:43

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  25. Content type: Research

    Quantitative real-time reverse transcription-polymerase chain reaction has been widely used in gene expression analysis, however, to have reliable and accurate results, reference genes are necessary to normali...

    Authors: Wenxian Liang, Xiaoxing Zou, Rebeca Carballar-Lejarazú, Lingjiao Wu, Weihong Sun, Xueyuan Yuan, Songqing Wu, Pengfei Li, Hui Ding, Lin Ni, Wei Huang and Shuangquan Zou

    Citation: Plant Methods 2018 14:42

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  26. Content type: Research

    The essential oil is an important compound of the root and rhizome of medicinally used valerian (Valeriana officinalis L. s.l.), with a stated minimum content in the European pharmacopoeia. The essential oil is l...

    Authors: Michael Penzkofer, Andrea Baron, Annette Naumann, Andrea Krähmer, Hartwig Schulz and Heidi Heuberger

    Citation: Plant Methods 2018 14:41

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  27. Content type: Methodology

    The CRISPR/Cas9 system is being used for genome editing purposes by many research groups in multiple plant species. Traditional sequencing methods to identify homozygous mutants are time-consuming, laborious a...

    Authors: Jinggong Guo, Kun Li, Lifeng Jin, Rui Xu, Kaiting Miao, Fengbo Yang, Chaoya Qi, Lin Zhang, Jose R. Botella, Ran Wang and Yuchen Miao

    Citation: Plant Methods 2018 14:40

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  28. Content type: Methodology

    One of the main challenges associated with image-based field phenotyping is the variability of illumination. During a single day’s imaging session, or between different sessions on different days, the sun move...

    Authors: Joshua Chopin, Pankaj Kumar and Stanley J. Miklavcic

    Citation: Plant Methods 2018 14:39

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  29. Content type: Methodology

    Reverse genetic strategies, such as virus-induced gene silencing, are powerful techniques to study gene function. Currently, there are few tools to study the spatial dependence of the consequences of gene sile...

    Authors: Sadie J. Burkhow, Nicole M. Stephens, Yu Mei, Maria Emilia Dueñas, Daniel J. Freppon, Geng Ding, Shea C. Smith, Young-Jin Lee, Basil J. Nikolau, Steven A. Whitham and Emily A. Smith

    Citation: Plant Methods 2018 14:37

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  30. Content type: Methodology

    When a plant is wounded, a rapid hydraulic surge, acting probably as a systemic signal, spreads from the site of injury throughout the plant and leads to small transient deformation of tissues. So far, the pro...

    Authors: Vladimíra Nožková, Petr Šmíd, Pavel Horváth, Miroslav Hrabovský and Petr Ilík

    Citation: Plant Methods 2018 14:38

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  31. Content type: Methodology

    Plant breeding requires numerous generations to be cycled and evaluated before an improved cultivar is released. This lengthy process is required to introduce and test multiple traits of interest. However, a t...

    Authors: Samir Alahmad, Eric Dinglasan, Kung Ming Leung, Adnan Riaz, Nora Derbal, Kai P. Voss-Fels, Jason A. Able, Filippo M. Bassi, Jack Christopher and Lee T. Hickey

    Citation: Plant Methods 2018 14:36

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  32. Content type: Methodology

    Solanum lycopersicum, an economically important crop grown worldwide, has been used as a model for the study of arbuscular mycorrhizal (AM) symbiosis in non-legume plants for several years and several cDNA array ...

    Authors: Tania Ho-Plágaro, Raúl Huertas, María I. Tamayo-Navarrete, Juan A. Ocampo and José M. García-Garrido

    Citation: Plant Methods 2018 14:34

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  33. Content type: Research

    Systemin has been extensively studied since it was discovered and is described as a peptidic hormone in tomato plants and other Solanaceae. Jasmonic acid and systemin are proposed to act through a positive feed-b...

    Authors: Victoria Pastor, Paloma Sánchez-Bel, Jordi Gamir, María J. Pozo and Víctor Flors

    Citation: Plant Methods 2018 14:33

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  34. Content type: Methodology

    The formation of crossovers during meiosis is pivotal for the redistribution of traits among the progeny of sexually reproducing organisms. In plants the molecular mechanisms underlying the formation of crosso...

    Authors: Niels van Tol, Martijn Rolloos, Peter van Loon and Bert J. van der Zaal

    Citation: Plant Methods 2018 14:32

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  35. Content type: Research

    For in vitro culture of plant and animal cells, one of the critical steps is to adjust the initial cell density. A typical example of this is isolated microspore culture, where specific cell densities have bee...

    Authors: Carolina Camacho-Fernández, David Hervás, Alba Rivas-Sendra, Mª Pilar Marín and Jose M. Seguí-Simarro

    Citation: Plant Methods 2018 14:30

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  36. Content type: Research

    Anthocyanins are abundant secondary metabolites responsible for most blue to blue-black, and red to purple colors of various plant organs. In wheat grains, anthocyanins are accumulated in the pericarp and/or a...

    Authors: Stefan Böhmdorfer, Josua Timotheus Oberlerchner, Christina Fuchs, Thomas Rosenau and Heinrich Grausgruber

    Citation: Plant Methods 2018 14:29

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  37. Content type: Methodology

    PCR allelic discrimination technologies have broad applications in the detection of single nucleotide polymorphisms (SNPs) in genetics and genomics. The use of fluorescence-tagged probes is the leading method ...

    Authors: Chiara Broccanello, Claudia Chiodi, Andrew Funk, J. Mitchell McGrath, Lee Panella and Piergiorgio Stevanato

    Citation: Plant Methods 2018 14:28

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  38. Content type: Methodology

    Accurate and automated phenotyping of leaf images is necessary for high throughput studies of leaf form like genome-wide association analysis and other forms of quantitative trait locus mapping. Dissected leav...

    Authors: Henrik Failmezger, Janne Lempe, Nasim Khadem, Maria Cartolano, Miltos Tsiantis and Achim Tresch

    Citation: Plant Methods 2018 14:27

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  39. Content type: Research

    Guard cell protoplasts (GCPs) isolated from various plants have proven to be especially useful for studies of signal transduction pathways and plant development. But it is not easy to isolate highly purified p...

    Authors: Xuehui Yao, Wenchao Zhao, Rui Yang, Jianli Wang, Fukuan Zhao and Shaohui Wang

    Citation: Plant Methods 2018 14:26

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  40. Content type: Methodology

    The current state-of-the-art for field wood identification to combat illegal logging relies on experienced practitioners using hand lenses, specialized identification keys, atlases of woods, and field manuals....

    Authors: Prabu Ravindran, Adriana Costa, Richard Soares and Alex C. Wiedenhoeft

    Citation: Plant Methods 2018 14:25

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  41. Content type: Research

    Leaf biochemical composition corresponds to traits related to the plant state and its functioning. This study puts the emphasis on the main leaf absorbers: chlorophyll a and b ( ...

    Authors: Jingyi Jiang, Alexis Comar, Philippe Burger, Pierre Bancal, Marie Weiss and Frédéric Baret

    Citation: Plant Methods 2018 14:23

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  42. Content type: Methodology

    The number of ears per unit ground area (ear density) is one of the main agronomic yield components in determining grain yield in wheat. A fast evaluation of this attribute may contribute to monitoring the eff...

    Authors: Jose A. Fernandez-Gallego, Shawn C. Kefauver, Nieves Aparicio Gutiérrez, María Teresa Nieto-Taladriz and José Luis Araus

    Citation: Plant Methods 2018 14:22

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  43. Content type: Methodology

    Unmanned aerial vehicles offer the opportunity for precision agriculture to efficiently monitor agricultural land. A vegetation index (VI) derived from an aerially observed multispectral image (MSI) can quanti...

    Authors: Zohaib Khan, Vahid Rahimi-Eichi, Stephan Haefele, Trevor Garnett and Stanley J. Miklavcic

    Citation: Plant Methods 2018 14:20

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