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  1. Research

    Ground penetrating radar: a case study for estimating root bulking rate in cassava (Manihot esculenta Crantz)

    Understanding root traits is a necessary research front for selection of favorable genotypes or cultivation practices. Root and tuber crops having most of their economic potential stored below ground are favor...

    Alfredo Delgado, Dirk B. Hays, Richard K. Bruton, Hernán Ceballos, Alexandre Novo, Enrico Boi and Michael Gomez Selvaraj

    Plant Methods 2017 13:65

    Published on: 7 August 2017

  2. Research

    Quantitation of glutathione S-transferases in rice (Oryza sativa L.) roots exposed to cadmium by liquid chromatography–tandem mass spectrometry using isotope-labeled wing peptides as an internal standard

    Plant glutathione S-transferases (GSTs, EC are multifunctional enzymes involved in heavy metal cellular detoxification by conjugating the tripeptide (g-Glu-Cys-Gly) glutathione to heavy metals. Previous...

    Zhenzhen Cao, Renxiang Mou, Zhaoyun Cao, Xiaoyan Lin, Youning Ma, Zhiwei Zhu and Mingxue Chen

    Plant Methods 2017 13:64

    Published on: 4 August 2017

  3. Methodology article

    An improved butanol-HCl assay for quantification of water-soluble, acetone:methanol-soluble, and insoluble proanthocyanidins (condensed tannins)

    Condensed tannins (CT) are the most abundant secondary metabolite of land plants and can vary in abundance and structure according to tissue type, species, genotype, age, and environmental conditions. Recent i...

    Philip-Edouard Shay, J. A. Trofymow and C. Peter Constabel

    Plant Methods 2017 13:63

    Published on: 2 August 2017

  4. Methodology article

    Genomic Bayesian functional regression models with interactions for predicting wheat grain yield using hyper-spectral image data

    Modern agriculture uses hyperspectral cameras that provide hundreds of reflectance data at discrete narrow bands in many environments. These bands often cover the whole visible light spectrum and part of the i...

    Abelardo Montesinos-López, Osval A. Montesinos-López, Jaime Cuevas, Walter A. Mata-López, Juan Burgueño, Sushismita Mondal, Julio Huerta, Ravi Singh, Enrique Autrique, Lorena González-Pérez and José Crossa

    Plant Methods 2017 13:62

    Published on: 27 July 2017

  5. Methodology article

    A procedure for maize genotypes discrimination to drought by chlorophyll fluorescence imaging rapid light curves

    Photosynthesis can be roughly separated into biochemical and photochemical processes. Both are affected by drought and can be assessed by non-invasive standard methods. Gas exchange, which mainly assesses the ...

    Carlos Antônio Ferreira de Sousa, Dayane Silva de Paiva, Raphael Augusto das Chagas Noqueli Casari, Nelson Geraldo de Oliveira, Hugo Bruno Correa Molinari, Adilson Kenji Kobayashi, Paulo Cesar Magalhães, Reinaldo Lúcio Gomide and Manoel Teixeira Souza Jr.

    Plant Methods 2017 13:61

    Published on: 26 July 2017

  6. Methodology article

    Elucidating the role of highly homologous Nicotiana benthamiana ubiquitin E2 gene family members in plant immunity through an improved virus-induced gene silencing approach

    Virus-induced gene silencing (VIGS) has been used in many plant species as an attractive post transcriptional gene silencing (PTGS) method for studying gene function either individually or at large-scale in a ...

    Bangjun Zhou and Lirong Zeng

    Plant Methods 2017 13:59

    Published on: 21 July 2017

  7. Methodology

    Accelerating root system phenotyping of seedlings through a computer-assisted processing pipeline

    There are numerous systems and techniques to measure the growth of plant roots. However, phenotyping large numbers of plant roots for breeding and genetic analyses remains challenging. One major difficulty is ...

    Lionel X. Dupuy, Gladys Wright, Jacqueline A. Thompson, Anna Taylor, Sebastien Dekeyser, Christopher P. White, William T. B. Thomas, Mark Nightingale, John P. Hammond, Neil S. Graham, Catherine L. Thomas, Martin R. Broadley and Philip J. White

    Plant Methods 2017 13:57

    Published on: 13 July 2017

  8. Methodology

    Development of a phenotyping platform for high throughput screening of nodal root angle in sorghum

    In sorghum, the growth angle of nodal roots is a major component of root system architecture. It strongly influences the spatial distribution of roots of mature plants in the soil profile, which can impact dro...

    Dinesh C. Joshi, Vijaya Singh, Colleen Hunt, Emma Mace, Erik van Oosterom, Richard Sulman, David Jordan and Graeme Hammer

    Plant Methods 2017 13:56

    Published on: 11 July 2017

  9. Methodology article

    Quantifying pruning impacts on olive tree architecture and annual canopy growth by using UAV-based 3D modelling

    Tree pruning is a costly practice with important implications for crop harvest and nutrition, pest and disease control, soil protection and irrigation strategies. Investigations on tree pruning usually involve...

    F. M. Jiménez-Brenes, F. López-Granados, A. I. de Castro, J. Torres-Sánchez, N. Serrano and J. M. Peña

    Plant Methods 2017 13:55

    Published on: 6 July 2017

  10. Research

    A mobile NMR lab for leaf phenotyping in the field

    Low field NMR has been used to investigate water status in various plant tissues. In plants grown in controlled conditions, the method was shown to be able to monitor leaf development as it could detect slight...

    Maja Musse, Laurent Leport, Mireille Cambert, William Debrandt, Clément Sorin, Alain Bouchereau and François Mariette

    Plant Methods 2017 13:53

    Published on: 28 June 2017

  11. Research

    Non-invasive absolute measurement of leaf water content using terahertz quantum cascade lasers

    Plant water resource management is one of the main future challenges to fight recent climatic changes. The knowledge of the plant water content could be indispensable for water saving strategies. Terahertz spe...

    Lorenzo Baldacci, Mario Pagano, Luca Masini, Alessandra Toncelli, Giorgio Carelli, Paolo Storchi and Alessandro Tredicucci

    Plant Methods 2017 13:51

    Published on: 17 June 2017

  12. Research

    Real-time monitoring of PtaHMGB activity in poplar transactivation assays

    Precise control of gene expression is essential to synchronize plant development with the environment. In perennial plants, transcriptional regulation remains poorly understood, mainly due to the long time req...

    José M. Ramos-Sánchez, Paolo M. Triozzi, Alicia Moreno-Cortés, Daniel Conde, Mariano Perales and Isabel Allona

    Plant Methods 2017 13:50

    Published on: 15 June 2017

  13. Methodology

    In vivo label-free mapping of the effect of a photosystem II inhibiting herbicide in plants using chlorophyll fluorescence lifetime

    In order to better understand and improve the mode of action of agrochemicals, it is useful to be able to visualize their uptake and distribution in vivo, non-invasively and, ideally, in the field. Here we exp...

    Elizabeth Noble, Sunil Kumar, Frederik G. Görlitz, Chris Stain, Chris Dunsby and Paul M. W. French

    Plant Methods 2017 13:48

    Published on: 15 June 2017

  14. Methodology

    Improved classification accuracy of powdery mildew infection levels of wine grapes by spatial-spectral analysis of hyperspectral images

    Hyperspectral imaging is an emerging means of assessing plant vitality, stress parameters, nutrition status, and diseases. Extraction of target values from the high-dimensional datasets either relies on pixel-...

    Uwe Knauer, Andrea Matros, Tijana Petrovic, Timothy Zanker, Eileen S. Scott and Udo Seiffert

    Plant Methods 2017 13:47

    Published on: 15 June 2017

  15. Methodology

    A multiplex PCR for rapid identification of Brassica species in the triangle of U

    Within the Brassicaceae, six species from the genus Brassica are widely cultivated throughout the world as oilseed, condiment, fodder or vegetable crops. The genetic relationships among the six Brassica species a...

    Joshua C. O. Koh, Denise M. Barbulescu, Sally Norton, Bob Redden, Phil A. Salisbury, Sukhjiwan Kaur, Noel Cogan and Anthony T. Slater

    Plant Methods 2017 13:49

    Published on: 15 June 2017

  16. Methodology

    An efficient protocol for perennial ryegrass mesophyll protoplast isolation and transformation, and its application on interaction study between LpNOL and LpNYC1

    Perennial ryegrass (Lolium perenne L.) is an important temperate grass used for turf and forage purposes. With the increasing accumulation of genomic and transcriptomic data of perennial ryegrass, an efficient pr...

    Guohui Yu, Qiang Cheng, Zheni Xie, Bin Xu, Bingru Huang and Bingyu Zhao

    Plant Methods 2017 13:46

    Published on: 5 June 2017

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