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  2. DNA–protein interactions are essential for several molecular and cellular mechanisms, such as transcription, transcriptional regulation, DNA modifications, among others. For many decades scientists tried to un...

    Authors: Ricardo André Campos Ferraz, Ana Lúcia Gonçalves Lopes, Jessy Ariana Faria da Silva, Diana Filipa Viana Moreira, Maria João Nogueira Ferreira and Sílvia Vieira de Almeida Coimbra

    Citation: Plant Methods 2021 17:82

    Content type: Review

    Published on:

  3. Drought is a major consequence of global heating that has negative impacts on agriculture. Potato is a drought-sensitive crop; tuber growth and dry matter content may both be impacted. Moreover, water deficit ...

    Authors: M. Musse, G. Hajjar, N. Ali, B. Billiot, G. Joly, J. Pépin, S. Quellec, S. Challois, F. Mariette, M. Cambert, C. Fontaine, C. Ngo-Dinh, F. Jamois, A. Barbary, P. Leconte, C. Deleu…

    Citation: Plant Methods 2021 17:81

    Content type: Research

    Published on:

  4. The parasitic plant Orobanche cumana is one of the most important threats to sunflower crops in Europe. Resistant sunflower varieties have been developed, but new O. cumana races have evolved and have overcome in...

    Authors: A. Le Ru, G. Ibarcq, M.- C. Boniface, A. Baussart, S. Muños and M. Chabaud

    Citation: Plant Methods 2021 17:80

    Content type: Research

    Published on:

  8. Sugarcane (Saccharum officinarum L.) is an economically important crop with stalks as the harvest organs. Improvement in stalk quality is deemed a promising strategy for enhancing sugarcane production. However, t...

    Authors: Maoyao Wang, Xinru Li, Yinjuan Shen, Muhammad Adnan, Le Mao, Pan Lu, Qian Hu, Fuhong Jiang, Muhammad Tahir Khan, Zuhu Deng, Baoshan Chen, Jiangfeng Huang and Muqing Zhang

    Citation: Plant Methods 2021 17:76

    Content type: Research

    Published on:

  9. Jujube is a typical fruit tree species from China. ‘Muzao’, a cracking-susceptible cultivar, and ‘Linhuang No. 1’, a cracking-resistant cultivar, were selected in a previous study as contrasting research mater...

    Authors: Na Li, Yuqin Song, Jie Li, Ruijie Hao, Xinxin Feng and Liulin Li

    Citation: Plant Methods 2021 17:75

    Content type: Research

    Published on:

  10. Cassava is an important crop for food security and industry in the least-developed and developing countries. The completion of the cassava genome sequence and identification of large numbers of candidate genes...

    Authors: Decai Tuo, Peng Zhou, Pu Yan, Hongguang Cui, Yang Liu, He Wang, Xiukun Yang, Wenbin Liao, Di Sun, Xiaoying Li and Wentao Shen

    Citation: Plant Methods 2021 17:74

    Content type: Methodology

    Published on:

  11. Agrobacterium-mediated genetic transformation is a widely used and efficient technique for gene functional research in crop breeding and plant biology. While in some plant species, including soybean, genetic tran...

    Authors: Yuanyuan Cheng, Xiaoli Wang, Li Cao, Jing Ji, Tengfei Liu and Kaixuan Duan

    Citation: Plant Methods 2021 17:73

    Content type: Methodology

    Published on:

  12. Surface roughness has a significant effect on leaf wettability. Consequently, it influences the efficiency and effectiveness of pesticide application. Therefore, roughness measurement of leaf surface offers su...

    Authors: Zeqing Zhang, Fei Liu, Zhenjiang Zhou, Yong He and Hui Fang

    Citation: Plant Methods 2021 17:72

    Content type: Research

    Published on:

  13. CRISPR/Cas9 is a rapidly developing genome editing technology in various biological systems due to its efficiency, portability, simplicity and versatility. This editing technology has been successfully applied...

    Authors: Wang Wang, Jiangmiao Liu, Hai Wang, Tong Li and Huien Zhao

    Citation: Plant Methods 2021 17:71

    Content type: Research

    Published on:

  14. Stipagrostis pennata (Trin.) De Winter is an important species for fixing sand in shifting and semi-fixed sandy lands, for grazing, and potentially as a source of lignocellulose fibres for pulp and paper industry...

    Authors: Masoumeh Asadi-Aghbolaghi, Beata Dedicova, Sonali Sachi Ranade, Kim-Cuong Le, Farzad Sharifzadeh, Mansoor Omidi and Ulrika Egertsdotter

    Citation: Plant Methods 2021 17:70

    Content type: Research

    Published on:

  15. Obtaining instantaneous gas exchanges data is fundamental to gain information on photosynthesis. Leaf level data are reliable, but their scaling up to canopy scale is difficult as they are acquired in standard...

    Authors: Nicole Salvatori, Alberti Giorgio, Onno Muller, Uwe Rascher and Alessandro Peressotti

    Citation: Plant Methods 2021 17:69

    Content type: Research

    Published on:

  17. Accurate and efficient measurement of the diameter at breast height (DBH) of individual trees is essential for forest inventories, ecological management, and carbon budget estimation. However, traditional diam...

    Authors: Chuangye Song, Bin Yang, Lin Zhang and Dongxiu Wu

    Citation: Plant Methods 2021 17:67

    Content type: Research

    Published on:

  18. Protocols for Agrobacterium rhizogenes-mediated hairy root transformation of the model legume Lotus japonicus have been established previously. However, little efforts were made in the past to quantify and improv...

    Authors: Yan Wang, Feng Yang, Peng-Fei Zhu, Asaf Khan, Zhi-Ping Xie and Christian Staehelin

    Citation: Plant Methods 2021 17:66

    Content type: Methodology

    Published on:

  19. The study of plant phenotype by deep learning has received increased interest in recent years, which impressive progress has been made in the fields of plant breeding. Deep learning extremely relies on a large...

    Authors: Ruisong Zhang, Ye Tian, Junmei Zhang, Silan Dai, Xiaogai Hou, Jue Wang and Qi Guo

    Citation: Plant Methods 2021 17:65

    Content type: Research

    Published on:

  20. To enforce timber import laws and perform timber species identification, the identity of the botanical species must be well-defined. Since the Sapotaceae family is known as a taxonomically challenging family, ...

    Authors: V. Deklerck, E. Price, S. Vanden Abeele, K. Lievens, E. Espinoza and H. Beeckman

    Citation: Plant Methods 2021 17:64

    Content type: Research

    Published on:

  23. Astragalus mongholicus Bunge is an important medicinal plant used in traditional Chinese medicine. It is rich in isoflavonoids and triterpenoid saponins. Although these active constituents of A. mongholicus have ...

    Authors: Minzhen Yin, Shanshan Chu, Tingyu Shan, Liangping Zha and Huasheng Peng

    Citation: Plant Methods 2021 17:61

    Content type: Research

    Published on:

  24. The fraction of intercepted photosynthetically active radiation (fPARi) is typically described with a non-linear function of leaf area index (LAI) and k, the light extinction coefficient. The parameter k is used ...

    Authors: Josefina Lacasa, Trevor J. Hefley, María E. Otegui and Ignacio A. Ciampitti

    Citation: Plant Methods 2021 17:60

    Content type: Methodology

    Published on:

  25. Taraxacum officinale, or the common dandelion, is a widespread perennial species recognized worldwide as a common lawn and garden weed. Common dandelion is also cultivated for use in teas, as edible greens, and f...

    Authors: Kasia Dinkeloo, Araceli Maria Cantero, Inyup Paik, Alexa Vulgamott, Andrew D Ellington and Alan Lloyd

    Citation: Plant Methods 2021 17:59

    Content type: Methodology

    Published on:

  26. Epicuticular wax (EW) is the first line of defense in plants for protection against biotic and abiotic factors in the environment. In wheat, EW is associated with resilience to heat and drought stress, however...

    Authors: Fátima Camarillo-Castillo, Trevis D. Huggins, Suchismita Mondal, Matthew P. Reynolds, Michael Tilley and Dirk B. Hays

    Citation: Plant Methods 2021 17:58

    Content type: Research

    Published on:

  28. Water content variation during plant growth is one of the most important monitoring parameters in plant studies. Conventional parameters (such as dry weight) are unreliable; thus, the development of rapid, acc...

    Authors: Yunjeong Yang, Ji Eun Kim, Hak Jin Song, Eun Bin Lee, Yong-Keun Choi, Jeong Wook Jo, Hyeon Jin Jeon, Ho Hyun Kim, Kwang Jin Kim and Hyung Joo Kim

    Citation: Plant Methods 2021 17:56

    Content type: Methodology

    Published on:

  29. Water uptake is essential for seed germination. However, Gleditsia sinensis seeds have a water-impermeable seed coat, which is beneficial for its adaption to the environment, but prohibits its germination without...

    Authors: Mingwei Zhu, Song Dai, Qiuyue Ma and Shuxian Li

    Citation: Plant Methods 2021 17:55

    Content type: Research

    Published on:

  30. Annotating gene structures and functions to genome assemblies is necessary to make assembly resources useful for biological inference. Gene Ontology (GO) term assignment is the most used functional annotation ...

    Authors: Kokulapalan Wimalanathan and Carolyn J. Lawrence-Dill

    Citation: Plant Methods 2021 17:54

    Content type: Software

    Published on:

  31. A variety of basic and applied research programs in plant biology require the accurate and reliable determination of plant tissue cold hardiness. Over the past 50 years, the electrolyte leakage method has emer...

    Authors: Alisson P. Kovaleski and Jake J. Grossman

    Citation: Plant Methods 2021 17:53

    Content type: Methodology

    Published on:

  33. Fractional vegetation cover (FVC) is an important parameter for evaluating crop-growth status. Optical remote-sensing techniques combined with the pixel dichotomy model (PDM) are widely used to estimate cropla...

    Authors: Jibo Yue, Wei Guo, Guijun Yang, Chengquan Zhou, Haikuan Feng and Hongbo Qiao

    Citation: Plant Methods 2021 17:51

    Content type: Methodology

    Published on:

  35. To accurately estimate winter wheat leaf area index (LAI) using unmanned aerial vehicle (UAV) hyperspectral imagery is crucial for crop growth monitoring, fertilization management, and development of precision...

    Authors: Juanjuan Zhang, Tao Cheng, Wei Guo, Xin Xu, Hongbo Qiao, Yimin Xie and Xinming Ma

    Citation: Plant Methods 2021 17:49

    Content type: Research

    Published on:

  36. Maize (Zea mays L.) is one of the most important food sources in the world and has been one of the main targets of plant genetics and phenotypic research for centuries. Observation and analysis of various morp...

    Authors: Shuo Zhou, Xiujuan Chai, Zixuan Yang, Hongwu Wang, Chenxue Yang and Tan Sun

    Citation: Plant Methods 2021 17:48

    Content type: Software

    Published on:

  38. Circadian rhythms regulate several physiological and developmental processes of plants. Hence, the identification of genes with the underlying circadian rhythmic features is pivotal. Though computational metho...

    Authors: Prabina Kumar Meher, Ansuman Mohapatra, Subhrajit Satpathy, Anuj Sharma, Isha Saini, Sukanta Kumar Pradhan and Anil Rai

    Citation: Plant Methods 2021 17:46

    Content type: Methodology

    Published on:

  39. The paper provides studies on the structure of planted small-leaved lime (Tilia cordata Mill.) in the conditions of the Bashkir Cis-Urals. This work aimed to analyze their assortment by diameters and compile appr...

    Authors: Aydar Gabdelkhakov, Zagir Rakhmatullin, Maria Martynova, Ilyas Fazlutdinov and Ilnur Mullagaleev

    Citation: Plant Methods 2021 17:45

    Content type: Research

    Published on:

  40. How to control the physical damage during maize kernel harvesting is a major problem for both mechanical designers and plant breeders. A limitation of addressing this problem is lacking a reliable method for a...

    Authors: Yuan Su, Yang Xu, Tao Cui, Xiaojun Gao, Guoyi Xia, Yibo Li, Mengmeng Qiao and Yingbo Yu

    Citation: Plant Methods 2021 17:44

    Content type: Research

    Published on:

  42. Syntrichia caninervis is a typical desiccation tolerant moss that is a dominant species forming biological soil crusts in the Gurbantunggut Desert. This study investigated the effect of different explants on rege...

    Authors: Xiujin Liu, Ping Zhou, Xiaoshuang Li and Daoyuan Zhang

    Citation: Plant Methods 2021 17:42

    Content type: Research

    Published on:

  43. Investigating plant mechanisms to tolerate freezing temperatures is critical to developing crops with superior cold hardiness. However, the lack of imaging methods that allow the visualization of freezing even...

    Authors: Camilo Villouta, Benjamin L. Cox, Beth Rauch, Beth Ann A. Workmaster, Kevin W. Eliceiri and Amaya Atucha

    Citation: Plant Methods 2021 17:41

    Content type: Methodology

    Published on:

  44. Crop yield is dependent on climate conditions, which are becoming both more variable and extreme in some areas of the world as a consequence of global climate change. Increased precipitation and flooding event...

    Authors: Alexandra Miricescu, Tomás Byrne, Catherine M. Doorly, Carl K. Y. Ng, Susanne Barth and Emmanuelle Graciet

    Citation: Plant Methods 2021 17:40

    Content type: Methodology

    Published on:

  45. X-ray computed tomography is acknowledged as a powerful tool for the study of root system architecture of plants growing in soil. In this paper, we improved the original root segmentation algorithm “Rootine” a...

    Authors: Maxime Phalempin, Eva Lippold, Doris Vetterlein and Steffen Schlüter

    Citation: Plant Methods 2021 17:39

    Content type: Methodology

    Published on:

  49. Wood basic density (WBD) is one of the most crucial wood property in tree and mainly determined the end use of wood for industry. However, the measurement WBD is time- and cost-consuming, which an alternativel...

    Authors: Yanjie Li, Wenjian Liu, Ruishu Cao, Zifeng Tan, Jun Liu and Jingmin Jiang

    Citation: Plant Methods 2021 17:35

    Content type: Research

    Published on:

  50. Plant traits related to nutrition have an influential role in tree growth, tree production and nutrient cycling. Therefore, the breeding program should consider the genetics of the traits. However, the measure...

    Authors: Jun Liu, Yang Sun, Wenjian Liu, Zifeng Tan, Jingmin Jiang and Yanjie Li

    Citation: Plant Methods 2021 17:33

    Content type: Research

    Published on:

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ISSN: 1746-4811