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  1. Improper management of agricultural and industrial cotton wastes causes environmental pollution and worsens the climate change challenge. Green recycling of cotton could contribute to a circular economy. One o...

    Authors: Mona A. Mohammed, Nagat M. Amer, Heba M. I. Abdallah and Mai S. Saleh
    Citation: Plant Methods 2024 20:54
  2. seeds that are recalcitrant to desiccation and freezing temperatures cannot be stored in gene banks under conventional conditions. However, the germplasm of some recalcitrant seeded species can be stored in li...

    Authors: Urszula Wasileńczyk, Mikołaj Krzysztof Wawrzyniak, João Paulo Rodrigues Martins, Paulina Kosek and Paweł Chmielarz
    Citation: Plant Methods 2024 20:53
  3. Salsola laricifolia is a typical C3–C4 typical desert plant, belonging to the family Amaranthaceae. An efficient single-cell system is crucial to study the gene function of this plant. In this study, we optimized...

    Authors: Hao Guo, Yuxin Xi, Kuerban Guzailinuer and Zhibin Wen
    Citation: Plant Methods 2024 20:52
  4. Stemphylium blight incited by Stemphylium botryosum poses a significant threat to lentil crops worldwide, inducing severe defoliation and causing substantial yield losses in susceptible varieties under favorable ...

    Authors: Mario González, Eleonora Barilli, Nicolas Rispail and Diego Rubiales
    Citation: Plant Methods 2024 20:51
  5. The root of a plant is a fundamental organ for the multisensory perception of the environment. Investigating root growth dynamics as a mean of their interaction with the environment is of key importance for im...

    Authors: Valentina Simonetti, Laura Ravazzolo, Benedetto Ruperti, Silvia Quaggiotti and Umberto Castiello
    Citation: Plant Methods 2024 20:50
  6. Leaf water content (LWC) significantly affects rice growth and development. Real-time monitoring of rice leaf water status is essential to obtain high yield and water use efficiency of rice plants with precise...

    Authors: Xuenan Zhang, Haocong Xu, Yehong She, Chao Hu, Tiezhong Zhu, Lele Wang, Liquan Wu, Cuicui You, Jian Ke, Qiangqiang Zhang and Haibing He
    Citation: Plant Methods 2024 20:48
  7. Cotton accounts for 80% of the global natural fibre production. Its leaf hairiness affects insect resistance, fibre yield, and economic value. However, this phenotype is still qualitatively assessed by visuall...

    Authors: Moshiur Farazi, Warren C. Conaty, Lucy Egan, Susan P. J. Thompson, Iain W. Wilson, Shiming Liu, Warwick N. Stiller, Lars Petersson and Vivien Rolland
    Citation: Plant Methods 2024 20:46
  8. For ten years, CRISPR/cas9 system has become a very useful tool for obtaining site-specific mutations on targeted genes in many plant organisms. This technology opens up a wide range of possibilities for impro...

    Authors: Jérémy Villette, Fatma Lecourieux, Eliot Bastiancig, Marie-Claire Héloir and Benoit Poinssot
    Citation: Plant Methods 2024 20:45
  9. Plant scientists have largely relied on pathogen growth assays and/or transcript analysis of stress-responsive genes for quantification of disease severity and susceptibility. These methods are destructive to ...

    Authors: Jae Hoon Lee, Unseok Lee, Ji Hye Yoo, Taek Sung Lee, Je Hyeong Jung and Hyoung Seok Kim
    Citation: Plant Methods 2024 20:44
  10. Dendrobium officinale is a medicinal plant with high commercial value. The Dendrobium officinale market in Yunnan is affected by the standardization of medicinal material quality control and the increase in marke...

    Authors: Peiyuan Li, Tao shen, Li Li and Yuanzhong Wang
    Citation: Plant Methods 2024 20:43
  11. Genomic selection (GS) has become an increasingly popular tool in plant breeding programs, propelled by declining genotyping costs, an increase in computational power, and rediscovery of the best linear unbias...

    Authors: Javier Fernández-González, Bertrand Haquin, Eliette Combes, Karine Bernard, Alix Allard and Julio Isidro y Sánchez
    Citation: Plant Methods 2024 20:42
  12. Gaseous phytohormone ethylene levels are directly influenced by the production of its immediate non-volatile precursor 1-aminocyclopropane-1-carboxylic acid (ACC). Owing to the strongly acidic character of the...

    Authors: Michal Karady, Pavel Hladík, Kateřina Cermanová, Petra Jiroutová, Ioanna Antoniadi, Rubén Casanova-Sáez, Karin Ljung and Ondřej Novák
    Citation: Plant Methods 2024 20:41
  13. Quantifying gene expression is a critical aspect of applied genomics research across all organisms, and real-time PCR has emerged as a powerful tool for this purpose. However, selecting appropriate internal co...

    Authors: Amin Ebrahimi, Shahrokh Gharanjik, Elham Azadvari and Sajad Rashidi-Monfared
    Citation: Plant Methods 2024 20:40
  14. Climate instability directly affects agro-environments. Water scarcity, high air temperature, and changes in soil biota are some factors caused by environmental changes. Verified and precise phenotypic traits ...

    Authors: Duvan Pineda-Castro, Harold Diaz, Jonatan Soto and Milan Oldřich Urban
    Citation: Plant Methods 2024 20:39
  15. The extraction of thylakoids is an essential step in studying the structure of photosynthetic complexes and several other aspects of the photosynthetic process in plants. Conventional protocols have been devel...

    Authors: Quentin Charras, Pascal Rey, Dorian Guillemain, Fabian Dourguin, Hugo Laganier, Sacha Peschoux, Roland Molinié, Marwa Ismaël, Stefano Caffarri, Catherine Rayon and Colette Jungas
    Citation: Plant Methods 2024 20:38
  16. Interspecific hybridisation is a powerful tool for increasing genetic diversity in plant breeding programmes. Hexaploid wheat (Triticum aestivum, 2n = 42) × barley (Hordeum vulgare, 2n = 14) intergeneric hybrids ...

    Authors: Mohammad Ali, Dávid Polgári, Adél Sepsi, Levente Kontra, Ágnes Dalmadi, Zoltán Havelda, László Sági and András Kis
    Citation: Plant Methods 2024 20:37
  17. Crop genetic engineering for better root systems can offer practical solutions for food security and carbon sequestration; however, soil layers prevent the direct visualization of plant roots, thus posing a ch...

    Authors: Mika Tei, Fumiyuki Soma, Ettore Barbieri, Yusaku Uga and Yosuke Kawahito
    Citation: Plant Methods 2024 20:36
  18. The development and physiology of plants are influenced by light intensity and its changes. Despite the significance of this phenomenon, there is a lack of understanding regarding the processes light regulates...

    Authors: Ludovico Caracciolo, John Philippi, Tom P. J. M. Theeuwen, Herbert van Amerongen and Jeremy Harbinson
    Citation: Plant Methods 2024 20:35
  19. More and more studies show that miRNA plays a crucial role in plants' response to different abiotic stresses. However, traditional experimental methods are often expensive and inefficient, so it is important t...

    Authors: Liming Chang, Xiu Jin, Yuan Rao and Xiaodan Zhang
    Citation: Plant Methods 2024 20:33
  20. Plant physiology and structure are constantly changing according to internal and external factors. The study of plant water dynamics can give information on these changes, as they are linked to numerous plant ...

    Authors: Shannan Blystone, Magali Nuixe, Amidou Sissou Traoré, Hervé Cochard, Catherine Picon-Cochard and Guilhem Pagés
    Citation: Plant Methods 2024 20:31
  21. The use of high-performant varieties could help to improve the production of food legumes and thus meet the demand of the growing world population. However, long periods needed to develop new varieties through...

    Authors: Mohammed Mitache, Aziz Baidani, Bouchaib Bencharki and Omar Idrissi
    Citation: Plant Methods 2024 20:30
  22. Hairy roots constitute a valuable tissue culture system for species that are difficult to propagate through conventional seed-based methods. Moreover, the generation of transgenic plants derived from hairy roo...

    Authors: Veronika Jedličková, Marie Štefková, Terezie Mandáková, Juan Francisco Sánchez López, Marek Sedláček, Martin A. Lysak and Hélène S. Robert
    Citation: Plant Methods 2024 20:29
  23. The challenge of pigeonpea breeding lies in its photosensitivity and seasonal specificity. This poses a problem to the breeder, as it restricts to single generation advancement in a year. Currently, the cross ...

    Authors: Prakash I. Gangashetty, Shruthi H. Belliappa, Naresh Bomma, Vinutha Kanuganahalli, Sobhan Babu Sajja, Sunita Choudhary, Ramanagouda Gaviyappanavar, Deekshitha Bomireddy, V. Anil Kumar, Jwala Pranati, Mamta Sharma and Manish K. Pandey
    Citation: Plant Methods 2024 20:27
  24. Virus-induced gene silencing (VIGS) is widely used in plant functional genomics. However, the efficiency of VIGS in young plantlets varies across plant species. Additionally, VIGS is not optimized for many pla...

    Authors: Yongliang Liu, Ruiqing Lyu, Joshua J. Singleton, Barunava Patra, Sitakanta Pattanaik and Ling Yuan
    Citation: Plant Methods 2024 20:26
  25. Mastering the spatial distribution and planting area of paddy can provide a scientific basis for monitoring rice production, and planning grain production layout. Previous remote sensing studies on paddy conce...

    Authors: Lihua Wang, Hao Ma, Yanghua Gao, Shengbo Chen, Songling Yang, Peng Lu, Li Fan and Yumiao Wang
    Citation: Plant Methods 2024 20:25
  26. Camellia oleifera, an essential woody oil tree in China, propagates through grafting. However, in production, it has been found that the interaction between rootstocks and scions may affect fruit characteristics....

    Authors: Fan Yang, Yuhuan Zhou, Jiayi Du, Kailiang Wang, Leyan Lv and Wei Long
    Citation: Plant Methods 2024 20:23
  27. The phenotypic traits of leaves are the direct reflection of the agronomic traits in the growth process of leafy vegetables, which plays a vital role in the selection of high-quality leafy vegetable varieties....

    Authors: Ziran Ye, Xiangfeng Tan, Mengdi Dai, Xuting Chen, Yuanxiang Zhong, Yi Zhang, Yunjie Ruan and Dedong Kong
    Citation: Plant Methods 2024 20:22
  28. The leaf angle distribution (LAD) is an important structural parameter of agricultural crops that influences light interception, radiation fluxes and consequently plant performance. Therefore, LAD and its para...

    Authors: Marie Theiß, Angelina Steier, Uwe Rascher and Mark Müller-Linow
    Citation: Plant Methods 2024 20:21
  29. The study aimed to edit ethylene (ET) biosynthesis genes [1-aminocyclopropane-1-carboxylic acid (ACC) synthetase 1 (ACS1) and ACC oxidase 1 (ACO1)] in carnation using the CRISPR/Cas9 ribonucleoprotein (RNP) compl...

    Authors: Oluwaseun Suleimon Adedeji, Aung Htay Naing, Hyunhee Kang, Junping Xu, Mi Young Chung and Chang Kil Kim
    Citation: Plant Methods 2024 20:20
  30. Herbaria are becoming increasingly important as archives of biodiversity, and play a central role in taxonomic and biogeographic studies. There is also an ongoing interest in functional traits and the way they...

    Authors: Paul Kühn, Tobias Proß, Christine Römermann, Karsten Wesche and Helge Bruelheide
    Citation: Plant Methods 2024 20:19
  31. Investigations on plant-pathogen interactions require quantitative, accurate, and rapid phenotyping of crop diseases. However, visual assessment of disease symptoms is preferred over available numerical tools ...

    Authors: Laura Mathieu, Maxime Reder, Ali Siah, Aurélie Ducasse, Camilla Langlands-Perry, Thierry C. Marcel, Jean-Benoît Morel, Cyrille Saintenac and Elsa Ballini
    Citation: Plant Methods 2024 20:18
  32. The low efficiency of genetic transformation in Chinese cabbage (Brassica rapa L. ssp. pekinensis) is the key problem affecting functional verification. Particle bombardment is a widely used method along with the...

    Authors: Yujia Liu, Shujiang Zhang, Shifan Zhang, Hui Zhang, Guoliang Li, Rifei Sun and Fei Li
    Citation: Plant Methods 2024 20:17
  33. One of the levers towards alternative solutions to pesticides is to improve seed defenses against pathogens, but a better understanding is needed on the type and regulation of existing pathways during germinat...

    Authors: Benjamin Hubert, Muriel Marchi, Joseph Ly Vu, Camille Tranchant, Łukasz P. Tarkowski, Olivier Leprince and Julia Buitink
    Citation: Plant Methods 2024 20:16
  34. The number of seedlings is an important indicator that reflects the size of the wheat population during the seedling stage. Researchers increasingly use deep learning to detect and count wheat seedlings from u...

    Authors: Suwan Wang, Jianqing Zhao, Yucheng Cai, Yan Li, Xuerui Qi, Xiaolei Qiu, Xia Yao, Yongchao Tian, Yan Zhu, Weixing Cao and Xiaohu Zhang
    Citation: Plant Methods 2024 20:15
  35. The potential of plant-based sources of vernolic acid to provide agricultural producers with a market diversification opportunity and industrial manufacturers with a renewable, environmentally friendly chemica...

    Authors: Maram Istaitieh, Jim F. Todd, Rene C. Van Acker, Mohsen Yoosefzadeh-Najafabadi and Istvan Rajcan
    Citation: Plant Methods 2024 20:14
  36. The study of plant photosynthesis is essential for productivity and yield. Thanks to the development of high-throughput phenotyping (HTP) facilities, based on chlorophyll fluorescence imaging, photosynthetic t...

    Authors: Federico Jurado-Ruiz, Thu-Phuong Nguyen, Joseph Peller, María José Aranzana, Gerrit Polder and Mark G. M. Aarts
    Citation: Plant Methods 2024 20:11
  37. Cryopreservation makes it possible to preserve plant biodiversity for thousands of years in ex situ storage. The stepwise dehydration method is a simple and versatile cryopreservation technique based on the vi...

    Authors: Teresa Hazubska-Przybył, Mikołaj Krzysztof Wawrzyniak, Agata Obarska and Terezia Salaj
    Citation: Plant Methods 2024 20:10
  38. Lentil is an important pulse that contributes to global food security and the sustainability of farming systems. Hence, it is important to increase the production of this crop, especially in the context of cli...

    Authors: Mohammed Mitache, Aziz Baidani, Bouchaib Bencharki and Omar Idrissi
    Citation: Plant Methods 2024 20:9
  39. In drought periods, water use efficiency depends on the capacity of roots to extract water from deep soil. A semi-field phenotyping facility (RadiMax) was used to investigate above-ground and root traits in sp...

    Authors: Biructawit B. Tessema, Miguel A. Raffo, Xiangyu Guo, Simon F. Svane, Lene Krusell, Jens Due Jensen, Anja Karine Ruud, Marta Malinowska, Kristian Thorup-Kristensen and Just Jensen
    Citation: Plant Methods 2024 20:8
  40. Strategies to understand meiotic processes have relied on cytogenetic and mutant analysis. However, thus far in vitro meiosis induction is a bottleneck to laboratory-based plant breeding as factor(s) that swit...

    Authors: Tanner M. Cook, Eva Biswas, Somak Dutta, Siddique I. Aboobucker, Sara Hazinia and Thomas Lübberstedt
    Citation: Plant Methods 2024 20:7
  41. Water dropwort (Oenanthe javanica (Blume) DC), an aquatic perennial plant from the Apiaceae family, rich in dietary fibert, vitamins, and minerals. It usually grows in wet soils and water. Despite accumulating th...

    Authors: Zhen He, Shuangyu Sheng, Lingqi Wang, Tingting Dong, Kun Zhang and Liangjun Li
    Citation: Plant Methods 2024 20:6
  42. The measurement of root dielectric response is a useful non-destructive method to evaluate root growth and function. Previous studies tracked root development throughout the plant growing cycle by single-time ...

    Authors: Imre Cseresnyés, Anna Füzy, Sándor Kabos, Bettina Kelemen, Kálmán Rajkai and Tünde Takács
    Citation: Plant Methods 2024 20:5

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