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  1. Content type: Methodology

    The formation of crossovers during meiosis is pivotal for the redistribution of traits among the progeny of sexually reproducing organisms. In plants the molecular mechanisms underlying the formation of crosso...

    Authors: Niels van Tol, Martijn Rolloos, Peter van Loon and Bert J. van der Zaal

    Citation: Plant Methods 2018 14:32

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  2. Content type: Research

    For in vitro culture of plant and animal cells, one of the critical steps is to adjust the initial cell density. A typical example of this is isolated microspore culture, where specific cell densities have bee...

    Authors: Carolina Camacho-Fernández, David Hervás, Alba Rivas-Sendra, Mª Pilar Marín and Jose M. Seguí-Simarro

    Citation: Plant Methods 2018 14:30

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  3. Content type: Research

    Anthocyanins are abundant secondary metabolites responsible for most blue to blue-black, and red to purple colors of various plant organs. In wheat grains, anthocyanins are accumulated in the pericarp and/or a...

    Authors: Stefan Böhmdorfer, Josua Timotheus Oberlerchner, Christina Fuchs, Thomas Rosenau and Heinrich Grausgruber

    Citation: Plant Methods 2018 14:29

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  4. Content type: Methodology

    PCR allelic discrimination technologies have broad applications in the detection of single nucleotide polymorphisms (SNPs) in genetics and genomics. The use of fluorescence-tagged probes is the leading method ...

    Authors: Chiara Broccanello, Claudia Chiodi, Andrew Funk, J. Mitchell McGrath, Lee Panella and Piergiorgio Stevanato

    Citation: Plant Methods 2018 14:28

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  5. Content type: Methodology

    Accurate and automated phenotyping of leaf images is necessary for high throughput studies of leaf form like genome-wide association analysis and other forms of quantitative trait locus mapping. Dissected leav...

    Authors: Henrik Failmezger, Janne Lempe, Nasim Khadem, Maria Cartolano, Miltos Tsiantis and Achim Tresch

    Citation: Plant Methods 2018 14:27

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  6. Content type: Research

    Guard cell protoplasts (GCPs) isolated from various plants have proven to be especially useful for studies of signal transduction pathways and plant development. But it is not easy to isolate highly purified p...

    Authors: Xuehui Yao, Wenchao Zhao, Rui Yang, Jianli Wang, Fukuan Zhao and Shaohui Wang

    Citation: Plant Methods 2018 14:26

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  7. Content type: Methodology

    The current state-of-the-art for field wood identification to combat illegal logging relies on experienced practitioners using hand lenses, specialized identification keys, atlases of woods, and field manuals....

    Authors: Prabu Ravindran, Adriana Costa, Richard Soares and Alex C. Wiedenhoeft

    Citation: Plant Methods 2018 14:25

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  8. Content type: Research

    Leaf biochemical composition corresponds to traits related to the plant state and its functioning. This study puts the emphasis on the main leaf absorbers: chlorophyll a and b ( ...

    Authors: Jingyi Jiang, Alexis Comar, Philippe Burger, Pierre Bancal, Marie Weiss and Frédéric Baret

    Citation: Plant Methods 2018 14:23

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  9. Content type: Methodology

    The number of ears per unit ground area (ear density) is one of the main agronomic yield components in determining grain yield in wheat. A fast evaluation of this attribute may contribute to monitoring the eff...

    Authors: Jose A. Fernandez-Gallego, Shawn C. Kefauver, Nieves Aparicio Gutiérrez, María Teresa Nieto-Taladriz and José Luis Araus

    Citation: Plant Methods 2018 14:22

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  10. Content type: Methodology

    Unmanned aerial vehicles offer the opportunity for precision agriculture to efficiently monitor agricultural land. A vegetation index (VI) derived from an aerially observed multispectral image (MSI) can quanti...

    Authors: Zohaib Khan, Vahid Rahimi-Eichi, Stephan Haefele, Trevor Garnett and Stanley J. Miklavcic

    Citation: Plant Methods 2018 14:20

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  11. Content type: Methodology

    Virus induced gene silencing (VIGS) is a powerful genomics tool for interrogating the function of plant genes. Unfortunately, VIGS vectors often produce disease symptoms that interfere with the silencing pheno...

    Authors: C. R. Gedling, E. M. Ali, A. Gunadi, J. J. Finer, K. Xie, Y. Liu, N. Yoshikawa, F. Qu and A. E. Dorrance

    Citation: Plant Methods 2018 14:19

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  12. Content type: Methodology

    The unicellular charophycean green alga Penium margaritaceum has emerged as an appealing experimental organism in plant cell wall and cell biology research. Innovative practical approaches in the manipulation and...

    Authors: Sandra Cristina Raimundo, Iben Sørensen, Berke Tinaz, Eleanore Ritter, Jocelyn K. C. Rose and David S. Domozych

    Citation: Plant Methods 2018 14:18

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  13. Content type: Methodology

    Camellia sinensis var. sinensis is widely grown for tea beverages that possess significant health promoting effects. Studies on tea plant genetics and breeding are hindered due to its recalcitrance to Agrobacteri...

    Authors: Karthikeyan Alagarsamy, Lubobi Ferdinand Shamala and Shu Wei

    Citation: Plant Methods 2018 14:17

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  14. Content type: Methodology

    Storage starch in starchy seed influences the seed weight and texture, and determines its applications in food and nonfood industries. Starch granules from different plant sources have significantly different ...

    Authors: Lingxiao Zhao, Ting Pan, Dongwei Guo and Cunxu Wei

    Citation: Plant Methods 2018 14:16

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  15. Content type: Research

    Leaf chlorophyll content (LCC) provides valuable information about plant physiology. Most of the published chlorophyll vegetation indices at the leaf level have been based on the spectral characteristics of th...

    Authors: Shan Lu, Fan Lu, Wenqiang You, Zheyi Wang, Yu Liu and Kenji Omasa

    Citation: Plant Methods 2018 14:15

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  16. Content type: Methodology

    Lipidomics plays an important role in understanding plant adaptation to different stresses and improving our knowledge of the genes underlying lipid metabolism. Lipidomics involves lipid extraction, sample pre...

    Authors: Sunitha Shiva, Regina Enninful, Mary R. Roth, Pamela Tamura, Krishna Jagadish and Ruth Welti

    Citation: Plant Methods 2018 14:14

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  17. Content type: Research

    Stalk lodging is a serious challenge in the production of maize and sorghum. A comprehensive understanding of lodging will likely require accurate characterizations of the mechanical properties of such plants....

    Authors: Loay Al-Zube, Wenhuan Sun, Daniel Robertson and Douglas Cook

    Citation: Plant Methods 2018 14:11

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  18. Content type: Methodology

    Genome-wide characterization of tissue- or cell-specific gene expression is a recurrent bottleneck in biology. We have developed a sensitive approach based on ultra-low RNA sequencing coupled to laser assisted...

    Authors: Kaori Sakai, Ludivine Taconnat, Nero Borrega, Jennifer Yansouni, Véronique Brunaud, Christine Paysant-Le Roux, Etienne Delannoy, Marie-Laure Martin Magniette, Loïc Lepiniec, Jean Denis Faure, Sandrine Balzergue and Bertrand Dubreucq

    Citation: Plant Methods 2018 14:10

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  19. Content type: Methodology

    Due to the nature of viral RNA genomes, RNA viruses depend on many RNA-binding proteins (RBP) of viral and host origin for replication, dissemination and evasion of host RNA degradation pathways. Some viruses ...

    Authors: F. E. Marmisolle, M. L. García and C. A. Reyes

    Citation: Plant Methods 2018 14:9

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  20. Content type: Methodology

    The amino acid profile of plants is an important parameter in assessments of their growth potential, resource-use efficiency and/or quality as food and feed. Screening studies may involve large number of sampl...

    Authors: Rasmus Dahl-Lassen, Jan van Hecke, Henning Jørgensen, Christian Bukh, Birgit Andersen and Jan K. Schjoerring

    Citation: Plant Methods 2018 14:8

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