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  1. Transgenic plant suspension cells show economic potential for the production of valuable bioproducts. The sugar starvation-inducible rice αAmy3 promoter, together with its signal peptide, is widely applied to pro...

    Authors: Desyanti Saulina Sinaga, Shin-Lon Ho, Chung-An Lu, Su-May Yu and Li-Fen Huang

    Citation: Plant Methods 2021 17:99

    Content type: Research

    Published on:

  2. Apricot is cultivated worldwide because of its high nutritive content and strong adaptability. Its flesh is delicious and has a unique and pleasant aroma. Apricot kernel is also consumed as nuts. The genome of...

    Authors: Chen Chen, Huimin Liu, Ningning Gou, Mengzhen Huang, Wanyu Xu, Xuchun Zhu, Mingyu Yin, Haikun Bai, Lin Wang and Ta-na Wuyun

    Citation: Plant Methods 2021 17:98

    Content type: Database

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  3. Detailed datasets containing root system and its architecture in soil are required to improve understanding of resource capture by roots. However, most of the root study methods have paid little attention to m...

    Authors: Takuya Koyama, Shun Murakami, Toshihiko Karasawa, Masato Ejiri and Katsuhiro Shiono

    Citation: Plant Methods 2021 17:97

    Content type: Methodology

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  4. Fractional vegetation cover (FVC) is an important basic parameter for the quantitative monitoring of the alpine grassland ecosystem on the Qinghai-Tibetan Plateau. Based on unmanned aerial vehicle (UAV) acquis...

    Authors: Xingchen Lin, Jianjun Chen, Peiqing Lou, Shuhua Yi, Yu Qin, Haotian You and Xiaowen Han

    Citation: Plant Methods 2021 17:96

    Content type: Research

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  5. Ploidy manipulation is effective in seedless loquat breeding, in which flesh color is a key agronomic and economic trait. Few techniques are currently available for detecting the genotypes of polyploids in pla...

    Authors: Haiyan Wang, Jiangbo Dang, Di Wu, Zhongyi Xie, Shuang Yan, Jingnan Luo, Qigao Guo and Guolu Liang

    Citation: Plant Methods 2021 17:93

    Content type: Methodology

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  6. Translation is a tightly regulated process, controlling the rate of protein synthesis in cells. Ribosome sequencing (Ribo-Seq) is a recently developed tool for studying actively translated mRNA and can thus di...

    Authors: Amir Mahboubi, Nicolas Delhomme, Sara Häggström and Johannes Hanson

    Citation: Plant Methods 2021 17:92

    Content type: Methodology

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  7. Maize cobs are an important component of crop yield that exhibit a high diversity in size, shape and color in native landraces and modern varieties. Various phenotyping approaches were developed to measure mai...

    Authors: Lydia Kienbaum, Miguel Correa Abondano, Raul Blas and Karl Schmid

    Citation: Plant Methods 2021 17:91

    Content type: Methodology

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  8. Estimation of nitrate nitrogen (NO3–N) content in petioles is one of the key approaches for monitoring nitrogen (N) nutrition in crops. Rapid, non-destructive, and accurate evaluation of NO3–N contents in cotto...

    Authors: Zhiqiang Dong, Yang Liu, Baoxia Ci, Ming Wen, Minghua Li, Xi Lu, Xiaokang Feng, Shuai Wen and Fuyu Ma

    Citation: Plant Methods 2021 17:90

    Content type: Methodology

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  9. Since the introduction of studies on maize silage digestibility at the end of the nineteenth century, protocols to estimate dry matter digestibility have not stopped evolving. Since the early 1980s, the protoc...

    Authors: P.-L. Lopez-Marnet, S. Guillaume, M.-P. Jacquemot, M. Reymond and V. Méchin

    Citation: Plant Methods 2021 17:89

    Content type: Methodology

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  10. Rice is one of the most important grain crops worldwide. The accurate and dynamic monitoring of Leaf Area Index (LAI) provides important information to evaluate rice growth and production.

    Authors: Yan Gong, Kaili Yang, Zhiheng Lin, Shenghui Fang, Xianting Wu, Renshan Zhu and Yi Peng

    Citation: Plant Methods 2021 17:88

    Content type: Research

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  11. Hyperaccumulation of trace elements is a rare trait among plants which is being investigated to advance our understanding of the regulation of metal accumulation and applications in phytotechnologies. Noccaea cae...

    Authors: Lucas van der Zee, Amelia Corzo Remigio, Lachlan W. Casey, Imam Purwadi, Jitpanu Yamjabok, Antony van der Ent, Gert Kootstra and Mark G. M. Aarts

    Citation: Plant Methods 2021 17:86

    Content type: Methodology

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  12. Heterosis has been widely used in maize breeding. However, we know little about the heterotic quantitative trait loci and their roles in genomic prediction. In this study, we sought to identify heterotic quant...

    Authors: Tifu Zhang, Lu Jiang, Long Ruan, Yiliang Qian, Shuaiqiang Liang, Feng Lin, Haiyan Lu, Huixue Dai and Han Zhao

    Citation: Plant Methods 2021 17:85

    Content type: Research

    Published on:

  13. Plant protoplasts constitute unique single-cell systems that can be subjected to genomic, proteomic, and metabolomic analysis. An effective and sustainable method for preparing protoplasts from tea plants has ...

    Authors: Xue-feng Xu, Hai-yan Zhu, Yin-feng Ren, Can Feng, Zhi-hao Ye, Hui-mei Cai, Xiao-chun Wan and Chuan-yi Peng

    Citation: Plant Methods 2021 17:84

    Content type: Methodology

    Published on:

  14. DNA–protein interactions are essential for several molecular and cellular mechanisms, such as transcription, transcriptional regulation, DNA modifications, among others. For many decades scientists tried to un...

    Authors: Ricardo André Campos Ferraz, Ana Lúcia Gonçalves Lopes, Jessy Ariana Faria da Silva, Diana Filipa Viana Moreira, Maria João Nogueira Ferreira and Sílvia Vieira de Almeida Coimbra

    Citation: Plant Methods 2021 17:82

    Content type: Review

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  15. Drought is a major consequence of global heating that has negative impacts on agriculture. Potato is a drought-sensitive crop; tuber growth and dry matter content may both be impacted. Moreover, water deficit ...

    Authors: M. Musse, G. Hajjar, N. Ali, B. Billiot, G. Joly, J. Pépin, S. Quellec, S. Challois, F. Mariette, M. Cambert, C. Fontaine, C. Ngo-Dinh, F. Jamois, A. Barbary, P. Leconte, C. Deleu…

    Citation: Plant Methods 2021 17:81

    Content type: Research

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  16. The parasitic plant Orobanche cumana is one of the most important threats to sunflower crops in Europe. Resistant sunflower varieties have been developed, but new O. cumana races have evolved and have overcome in...

    Authors: A. Le Ru, G. Ibarcq, M.- C. Boniface, A. Baussart, S. Muños and M. Chabaud

    Citation: Plant Methods 2021 17:80

    Content type: Research

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  17. Sugarcane (Saccharum officinarum L.) is an economically important crop with stalks as the harvest organs. Improvement in stalk quality is deemed a promising strategy for enhancing sugarcane production. However, t...

    Authors: Maoyao Wang, Xinru Li, Yinjuan Shen, Muhammad Adnan, Le Mao, Pan Lu, Qian Hu, Fuhong Jiang, Muhammad Tahir Khan, Zuhu Deng, Baoshan Chen, Jiangfeng Huang and Muqing Zhang

    Citation: Plant Methods 2021 17:76

    Content type: Research

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  18. Jujube is a typical fruit tree species from China. ‘Muzao’, a cracking-susceptible cultivar, and ‘Linhuang No. 1’, a cracking-resistant cultivar, were selected in a previous study as contrasting research mater...

    Authors: Na Li, Yuqin Song, Jie Li, Ruijie Hao, Xinxin Feng and Liulin Li

    Citation: Plant Methods 2021 17:75

    Content type: Research

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  19. Cassava is an important crop for food security and industry in the least-developed and developing countries. The completion of the cassava genome sequence and identification of large numbers of candidate genes...

    Authors: Decai Tuo, Peng Zhou, Pu Yan, Hongguang Cui, Yang Liu, He Wang, Xiukun Yang, Wenbin Liao, Di Sun, Xiaoying Li and Wentao Shen

    Citation: Plant Methods 2021 17:74

    Content type: Methodology

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  20. Agrobacterium-mediated genetic transformation is a widely used and efficient technique for gene functional research in crop breeding and plant biology. While in some plant species, including soybean, genetic tran...

    Authors: Yuanyuan Cheng, Xiaoli Wang, Li Cao, Jing Ji, Tengfei Liu and Kaixuan Duan

    Citation: Plant Methods 2021 17:73

    Content type: Methodology

    Published on:

  21. Surface roughness has a significant effect on leaf wettability. Consequently, it influences the efficiency and effectiveness of pesticide application. Therefore, roughness measurement of leaf surface offers su...

    Authors: Zeqing Zhang, Fei Liu, Zhenjiang Zhou, Yong He and Hui Fang

    Citation: Plant Methods 2021 17:72

    Content type: Research

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  22. CRISPR/Cas9 is a rapidly developing genome editing technology in various biological systems due to its efficiency, portability, simplicity and versatility. This editing technology has been successfully applied...

    Authors: Wang Wang, Jiangmiao Liu, Hai Wang, Tong Li and Huien Zhao

    Citation: Plant Methods 2021 17:71

    Content type: Research

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  23. Stipagrostis pennata (Trin.) De Winter is an important species for fixing sand in shifting and semi-fixed sandy lands, for grazing, and potentially as a source of lignocellulose fibres for pulp and paper industry...

    Authors: Masoumeh Asadi-Aghbolaghi, Beata Dedicova, Sonali Sachi Ranade, Kim-Cuong Le, Farzad Sharifzadeh, Mansoor Omidi and Ulrika Egertsdotter

    Citation: Plant Methods 2021 17:70

    Content type: Research

    Published on:

  24. Obtaining instantaneous gas exchanges data is fundamental to gain information on photosynthesis. Leaf level data are reliable, but their scaling up to canopy scale is difficult as they are acquired in standard...

    Authors: Nicole Salvatori, Alberti Giorgio, Onno Muller, Uwe Rascher and Alessandro Peressotti

    Citation: Plant Methods 2021 17:69

    Content type: Research

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  25. Accurate and efficient measurement of the diameter at breast height (DBH) of individual trees is essential for forest inventories, ecological management, and carbon budget estimation. However, traditional diam...

    Authors: Chuangye Song, Bin Yang, Lin Zhang and Dongxiu Wu

    Citation: Plant Methods 2021 17:67

    Content type: Research

    Published on:

  26. Protocols for Agrobacterium rhizogenes-mediated hairy root transformation of the model legume Lotus japonicus have been established previously. However, little efforts were made in the past to quantify and improv...

    Authors: Yan Wang, Feng Yang, Peng-Fei Zhu, Asaf Khan, Zhi-Ping Xie and Christian Staehelin

    Citation: Plant Methods 2021 17:66

    Content type: Methodology

    Published on:

  27. The study of plant phenotype by deep learning has received increased interest in recent years, which impressive progress has been made in the fields of plant breeding. Deep learning extremely relies on a large...

    Authors: Ruisong Zhang, Ye Tian, Junmei Zhang, Silan Dai, Xiaogai Hou, Jue Wang and Qi Guo

    Citation: Plant Methods 2021 17:65

    Content type: Research

    Published on:

  28. To enforce timber import laws and perform timber species identification, the identity of the botanical species must be well-defined. Since the Sapotaceae family is known as a taxonomically challenging family, ...

    Authors: V. Deklerck, E. Price, S. Vanden Abeele, K. Lievens, E. Espinoza and H. Beeckman

    Citation: Plant Methods 2021 17:64

    Content type: Research

    Published on:

  29. Astragalus mongholicus Bunge is an important medicinal plant used in traditional Chinese medicine. It is rich in isoflavonoids and triterpenoid saponins. Although these active constituents of A. mongholicus have ...

    Authors: Minzhen Yin, Shanshan Chu, Tingyu Shan, Liangping Zha and Huasheng Peng

    Citation: Plant Methods 2021 17:61

    Content type: Research

    Published on:

  30. The fraction of intercepted photosynthetically active radiation (fPARi) is typically described with a non-linear function of leaf area index (LAI) and k, the light extinction coefficient. The parameter k is used ...

    Authors: Josefina Lacasa, Trevor J. Hefley, María E. Otegui and Ignacio A. Ciampitti

    Citation: Plant Methods 2021 17:60

    Content type: Methodology

    Published on:

  31. Taraxacum officinale, or the common dandelion, is a widespread perennial species recognized worldwide as a common lawn and garden weed. Common dandelion is also cultivated for use in teas, as edible greens, and f...

    Authors: Kasia Dinkeloo, Araceli Maria Cantero, Inyup Paik, Alexa Vulgamott, Andrew D Ellington and Alan Lloyd

    Citation: Plant Methods 2021 17:59

    Content type: Methodology

    Published on:

  32. Epicuticular wax (EW) is the first line of defense in plants for protection against biotic and abiotic factors in the environment. In wheat, EW is associated with resilience to heat and drought stress, however...

    Authors: Fátima Camarillo-Castillo, Trevis D. Huggins, Suchismita Mondal, Matthew P. Reynolds, Michael Tilley and Dirk B. Hays

    Citation: Plant Methods 2021 17:58

    Content type: Research

    Published on:

  33. Water content variation during plant growth is one of the most important monitoring parameters in plant studies. Conventional parameters (such as dry weight) are unreliable; thus, the development of rapid, acc...

    Authors: Yunjeong Yang, Ji Eun Kim, Hak Jin Song, Eun Bin Lee, Yong-Keun Choi, Jeong Wook Jo, Hyeon Jin Jeon, Ho Hyun Kim, Kwang Jin Kim and Hyung Joo Kim

    Citation: Plant Methods 2021 17:56

    Content type: Methodology

    Published on:

  34. Water uptake is essential for seed germination. However, Gleditsia sinensis seeds have a water-impermeable seed coat, which is beneficial for its adaption to the environment, but prohibits its germination without...

    Authors: Mingwei Zhu, Song Dai, Qiuyue Ma and Shuxian Li

    Citation: Plant Methods 2021 17:55

    Content type: Research

    Published on:

  35. Annotating gene structures and functions to genome assemblies is necessary to make assembly resources useful for biological inference. Gene Ontology (GO) term assignment is the most used functional annotation ...

    Authors: Kokulapalan Wimalanathan and Carolyn J. Lawrence-Dill

    Citation: Plant Methods 2021 17:54

    Content type: Software

    Published on:

  36. A variety of basic and applied research programs in plant biology require the accurate and reliable determination of plant tissue cold hardiness. Over the past 50 years, the electrolyte leakage method has emer...

    Authors: Alisson P. Kovaleski and Jake J. Grossman

    Citation: Plant Methods 2021 17:53

    Content type: Methodology

    Published on:

  37. Fractional vegetation cover (FVC) is an important parameter for evaluating crop-growth status. Optical remote-sensing techniques combined with the pixel dichotomy model (PDM) are widely used to estimate cropla...

    Authors: Jibo Yue, Wei Guo, Guijun Yang, Chengquan Zhou, Haikuan Feng and Hongbo Qiao

    Citation: Plant Methods 2021 17:51

    Content type: Methodology

    Published on:

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