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  1. Investigating plant mechanisms to tolerate freezing temperatures is critical to developing crops with superior cold hardiness. However, the lack of imaging methods that allow the visualization of freezing even...

    Authors: Camilo Villouta, Benjamin L. Cox, Beth Rauch, Beth Ann A. Workmaster, Kevin W. Eliceiri and Amaya Atucha

    Citation: Plant Methods 2021 17:41

    Content type: Methodology

    Published on:

  2. Crop yield is dependent on climate conditions, which are becoming both more variable and extreme in some areas of the world as a consequence of global climate change. Increased precipitation and flooding event...

    Authors: Alexandra Miricescu, Tomás Byrne, Catherine M. Doorly, Carl K. Y. Ng, Susanne Barth and Emmanuelle Graciet

    Citation: Plant Methods 2021 17:40

    Content type: Methodology

    Published on:

  3. X-ray computed tomography is acknowledged as a powerful tool for the study of root system architecture of plants growing in soil. In this paper, we improved the original root segmentation algorithm “Rootine” a...

    Authors: Maxime Phalempin, Eva Lippold, Doris Vetterlein and Steffen Schlüter

    Citation: Plant Methods 2021 17:39

    Content type: Methodology

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  7. Wood basic density (WBD) is one of the most crucial wood property in tree and mainly determined the end use of wood for industry. However, the measurement WBD is time- and cost-consuming, which an alternativel...

    Authors: Yanjie Li, Wenjian Liu, Ruishu Cao, Zifeng Tan, Jun Liu and Jingmin Jiang

    Citation: Plant Methods 2021 17:35

    Content type: Research

    Published on:

  9. Plant traits related to nutrition have an influential role in tree growth, tree production and nutrient cycling. Therefore, the breeding program should consider the genetics of the traits. However, the measure...

    Authors: Jun Liu, Yang Sun, Wenjian Liu, Zifeng Tan, Jingmin Jiang and Yanjie Li

    Citation: Plant Methods 2021 17:33

    Content type: Research

    Published on:

  10. We report a method to estimate carbon assimilation based on isotope ratio-mass spectrometry (IRMS) of 13CO2 labeled plant tissue. Photosynthetic carbon assimilation is the principal experimental observable which ...

    Authors: Matthew E. Bergman, Diego González-Cabanelas, Louwrance P. Wright, Berkley J. Walker and Michael A. Phillips

    Citation: Plant Methods 2021 17:32

    Content type: Methodology

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  11. In vitro culture of olive, as an economically valuable tree, has fundamentally a genotype-dependant low micropropagation rate which needs to be improved in already established and newly released cultivars. Var...

    Authors: Leila Mirzaei, Abbas Yadollahi, Maryam Jafarkhani Kermani, Masoud Naderpour, Ali Asghar Zeinanloo, Maryam Farsi and Dariush Davoodi

    Citation: Plant Methods 2021 17:31

    Content type: Research

    Published on:

  12. K-seq, a new genotyping methodology based on the amplification of genomic regions using two steps of Klenow amplification with short oligonucleotides, followed by standard PCR and Illumina sequencing, is prese...

    Authors: Peio Ziarsolo, Tomas Hasing, Rebeca Hilario, Victor Garcia-Carpintero, Jose Blanca, Aureliano Bombarely and Joaquin Cañizares

    Citation: Plant Methods 2021 17:30

    Content type: Methodology

    Published on:

  13. New cell wall imaging tools permit direct visualization of the molecular architecture of cell walls and provide detailed chemical information on wall polymers, which will aid efforts to use these polymers in m...

    Authors: Huimin Xu, Yuanyuan Zhao, Yuanzhen Suo, Yayu Guo, Yi Man, Yanping Jing, Xinqiang He and Jinxing Lin

    Citation: Plant Methods 2021 17:29

    Content type: Methodology

    Published on:

  16. Wheat spike architecture is a key determinant of multiple grain yield components and detailed examination of spike morphometric traits is beneficial to explain wheat grain yield and the effects of differing ag...

    Authors: Hu Zhou, Andrew B. Riche, Malcolm J. Hawkesford, William R. Whalley, Brian S. Atkinson, Craig J. Sturrock and Sacha J. Mooney

    Citation: Plant Methods 2021 17:26

    Content type: Methodology

    Published on:

  19. Floral temperature has important consequences for plant biology, and accurate temperature measurements are therefore important to plant research. Thermography, also referred to as thermal imaging, is beginning...

    Authors: Michael J. M. Harrap and Sean A. Rands

    Citation: Plant Methods 2021 17:23

    Content type: Research

    Published on:

  22. The virus-induced genome editing (VIGE) system can be used to quickly identify gene functions and generate knock-out libraries as an alternative to the virus-induced gene silencing (VIGS). Although plant virus...

    Authors: Jianfeng Lei, Peihong Dai, Yue Li, Wanqi Zhang, Guantong Zhou, Chao Liu and Xiaodong Liu

    Citation: Plant Methods 2021 17:20

    Content type: Methodology

    Published on:

  24. Dispensability of genes in a phylogenetic lineage, e.g. a species, genus, or higher-level clade, is gaining relevance as most genome sequencing projects move to a pangenome level. Most analyses classify genes ...

    Authors: Katharina Sielemann, Bernd Weisshaar and Boas Pucker

    Citation: Plant Methods 2021 17:18

    Content type: Methodology

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  26. Upland cotton (Gossypium hirsutum), harboring a complex allotetraploid genome, consists of A and D sub-genomes. Every gene has multiple copies with high sequence similarity that makes genetic, genomic and functio...

    Authors: Mohamed Ramadan, Muna Alariqi, Yizan Ma, Yanlong Li, Zhenping Liu, Rui Zhang, Shuangxia Jin, Ling Min and Xianlong Zhang

    Citation: Plant Methods 2021 17:16

    Content type: Methodology

    Published on:

  27. Casbene synthase (CS) is responsible for the first committed step in the biosynthesis of phorbol esters (PE) in the Euphorbiaceae. PE are abundant in the seeds of the biofuel crop Jatropha curcas and its toxicity...

    Authors: Natália Pinto de Almeida, Domingos Ferreira Mélo Neto, Gabriel Reis Alves Carneiro, Andreza Raquel Barbosa de Farias, Gilberto Barbosa Domont, Francisco de Assis de Paiva Campos and Fábio César Sousa Nogueira

    Citation: Plant Methods 2021 17:15

    Content type: Research

    Published on:

  28. The presence of condensed tannins (CT) in tree fodders entails a series of productive, health and ecological benefits for ruminant nutrition. Current wet analytical methods employed for full CT characterisatio...

    Authors: Jordi Ortuño, Sokratis Stergiadis, Anastasios Koidis, Jo Smith, Chris Humphrey, Lindsay Whistance and Katerina Theodoridou

    Citation: Plant Methods 2021 17:14

    Content type: Research

    Published on:

  29. Paclitaxel is a well-known chemotherapeutic agent widely applied as a therapy for various types of cancers. In vitro culture of Corylus avellana has been named as a promising and low-cost strategy for paclitaxel ...

    Authors: Mina Salehi, Siamak Farhadi, Ahmad Moieni, Naser Safaie and Mohsen Hesami

    Citation: Plant Methods 2021 17:13

    Content type: Research

    Published on:

  30. Pyropia is an economically advantageous genus of red macroalgae, which has been cultivated in the coastal areas of East Asia for over 300 years. Realizing estimation of macroalgae biomass in a high-throughput way...

    Authors: Shuai Che, Guoying Du, Ning Wang, Kun He, Zhaolan Mo, Bin Sun, Yu Chen, Yifei Cao, Junhao Wang and Yunxiang Mao

    Citation: Plant Methods 2021 17:12

    Content type: Methodology

    Published on:

  32. Hypericum is an important genus in the family Hypericaceae, which includes 484 species. This genus has been grown in temperate regions and used for treating wounds, eczema and burns. The aim of this study was to ...

    Authors: Maryam Saffariha, Ali Jahani, Reza Jahani and Sajid Latif

    Citation: Plant Methods 2021 17:10

    Content type: Research

    Published on:

  33. The use of non-destructive methods with less human interference is of great interest in agricultural industry and crop breeding. Modern imaging technologies enable the automatic visualization of multi-paramete...

    Authors: Vitor de Jesus Martins Bianchini, Gabriel Moura Mascarin, Lúcia Cristina Aparecida Santos Silva, Valter Arthur, Jens Michael Carstensen, Birte Boelt and Clíssia Barboza da Silva

    Citation: Plant Methods 2021 17:9

    Content type: Methodology

    Published on:

  34. The absorption, translocation, accumulation and excretion of substances are fundamental processes in all organisms including plants, and have been successfully studied using radiotracers labelled with 11C, 13N, 1...

    Authors: Gihan P. Ruwanpathirana, Darren C. Plett, Robert C. Williams, Catherine E. Davey, Leigh A. Johnston and Herbert J. Kronzucker

    Citation: Plant Methods 2021 17:8

    Content type: Methodology

    Published on:

  35. Split-root systems (SRS) have many applications in plant sciences, but their implementation, depending on the experimental design, can be difficult and time-consuming. Additionally, the system is not exempt fr...

    Authors: Iñigo Saiz-Fernández, Martin Černý, Jan Skalák and Břetislav Brzobohatý

    Citation: Plant Methods 2021 17:7

    Content type: Research

    Published on:

  36. Virus-induced gene silencing (VIGS) is a useful tool for functional characterizations of plant genes. However, the penetrance of VIGS varies depending on the genes to be silenced, and has to be evaluated by ex...

    Authors: Kunxin Wu, Yadan Wu, Chunwei Zhang, Yan Fu, Zhixin Liu and Xiuchun Zhang

    Citation: Plant Methods 2021 17:6

    Content type: Research

    Published on:

  37. Wind strongly impacts plant growth, leaf traits, biomass allocation, and stem mechanical properties. However, whether there are common whole-plant wind responses among different plant species is still unclear....

    Authors: Shudong Zhang, Guofang Liu, Qingguo Cui, Zhenying Huang, Xuehua Ye and Johannes H. C. Cornelissen

    Citation: Plant Methods 2021 17:5

    Content type: Research

    Published on:

  38. Photosynthetic pigments participating in the absorption, transformation and transfer of light energy play a very important role in plant growth. While, the spatial distribution of foliar pigments is an importa...

    Authors: Jianjun Zeng, Wen Ping, Alireza Sanaeifar, Xiao Xu, Wei Luo, Junjing Sha, Zhenxiong Huang, Yifeng Huang, Xuemei Liu, Baishao Zhan, Hailiang Zhang and Xiaoli Li

    Citation: Plant Methods 2021 17:4

    Content type: Research

    Published on:

  39. Enzyme assays have widespread applications in drug discovery from plants to natural products. The appropriate use of blanks in enzyme assays is important for assay baseline-correction, and the correction of fa...

    Authors: Chintha Lankatillake, Shiqi Luo, Matthew Flavel, George Binh Lenon, Harsharn Gill, Tien Huynh and Daniel Anthony Dias

    Citation: Plant Methods 2021 17:3

    Content type: Methodology

    Published on:

  40. Maize haploid breeding technology can be used to rapidly develop homozygous lines, significantly shorten the breeding cycle and improve breeding efficiency. Rapid and accurate sorting haploid kernels is a prer...

    Authors: Yanzhi Qu, Zonghua Liu, Yazhou Zhang, Jiwei Yang and Haochuan Li

    Citation: Plant Methods 2021 17:2

    Content type: Methodology

    Published on:

  41. Forest dieback driven by rapid climate warming threatens ecosystems worldwide. The health of forested ecosystems depends on how tree species respond to warming during all life history stages. While it is known...

    Authors: E. R. V. Moler, G. Page, L. Flores-Rentería, C. G. Garms, J. B. Hull, H. F. Cooper, J. Swenson, S. Perks, K. M. Waring and A. V. Whipple

    Citation: Plant Methods 2021 17:1

    Content type: Research

    Published on:

  42. Assessment of seed germination is an essential task for seed researchers to measure the quality and performance of seeds. Usually, seed assessments are done manually, which is a cumbersome, time consuming and ...

    Authors: Nikita Genze, Richa Bharti, Michael Grieb, Sebastian J. Schultheiss and Dominik G. Grimm

    Citation: Plant Methods 2020 16:157

    Content type: Methodology

    Published on:

  43. Flowering is one of the most important processes for flowering plants such as cotton, reflecting the transition from vegetative to reproductive growth and is of central importance to crop yield and adaptabilit...

    Authors: Yu Jiang, Changying Li, Rui Xu, Shangpeng Sun, Jon S. Robertson and Andrew H. Paterson

    Citation: Plant Methods 2020 16:156

    Content type: Methodology

    Published on:

  44. The plant lipidome is highly complex, and the composition of lipids in different tissues as well as their specific functions in plant development, growth and stress responses have yet to be fully elucidated. T...

    Authors: Cheka Kehelpannala, Thusitha W. T. Rupasinghe, Thomas Hennessy, David Bradley, Berit Ebert and Ute Roessner

    Citation: Plant Methods 2020 16:155

    Content type: Methodology

    Published on:

  45. Pentatricopeptide-repeat proteins (PPRs) characterized by tandem arrays of a degenerate 35-amino-acid repeat (PPR motif) can bind a single strand RNA and regulate organelle gene expression at the post-transcri...

    Authors: Jingli Chen, Haojie Zhu, Jirong Huang and Weihua Huang

    Citation: Plant Methods 2020 16:154

    Content type: Research

    Published on:

  47. Hormones are crucial to plant life and development. Being able to follow the plants hormonal response to various stimuli and throughout developmental processes is an important and increasingly widespread tool....

    Authors: Evyatar Steiner, Alon Israeli, Rupali Gupta, Ido Shwartz, Ido Nir, Meirav Leibman-Markus, Lior Tal, Mika Farber, Ziva Amsalem, Naomi Ori, Bruno Müller and Maya Bar

    Citation: Plant Methods 2020 16:152

    Content type: Methodology

    Published on:

  48. An efficient in vivo transient transfection system using protoplasts is an important tool to study gene expression, metabolic pathways, and multiple mutagenesis parameters in plants. Although rice protoplasts ...

    Authors: Snigdha Poddar, Jaclyn Tanaka, Jamie H. D. Cate, Brian Staskawicz and Myeong-Je Cho

    Citation: Plant Methods 2020 16:151

    Content type: Methodology

    Published on:

  49. The accurate estimation of potato yield at regional scales is crucial for food security, precision agriculture, and agricultural sustainable development.

    Authors: Shanjun Luo, Yingbin He, Qian Li, Weihua Jiao, Yaqiu Zhu and Xihai Zhao

    Citation: Plant Methods 2020 16:150

    Content type: Research

    Published on:

  50. The accurate evaluation of the stalk-lodging resistance during the late stage of maize growth can provide a basis for the selection of cultivars, the evaluation of cultivation techniques, and timely mechanical...

    Authors: Jun Xue, Bo Ming, Ruizhi Xie, Keru Wang, Peng Hou and Shaokun Li

    Citation: Plant Methods 2020 16:148

    Content type: Research

    Published on:

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