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  1. The fraction of intercepted photosynthetically active radiation (fPARi) is typically described with a non-linear function of leaf area index (LAI) and k, the light extinction coefficient. The parameter k is used ...

    Authors: Josefina Lacasa, Trevor J. Hefley, María E. Otegui and Ignacio A. Ciampitti

    Citation: Plant Methods 2021 17:60

    Content type: Methodology

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  2. Taraxacum officinale, or the common dandelion, is a widespread perennial species recognized worldwide as a common lawn and garden weed. Common dandelion is also cultivated for use in teas, as edible greens, and f...

    Authors: Kasia Dinkeloo, Araceli Maria Cantero, Inyup Paik, Alexa Vulgamott, Andrew D Ellington and Alan Lloyd

    Citation: Plant Methods 2021 17:59

    Content type: Methodology

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  3. Epicuticular wax (EW) is the first line of defense in plants for protection against biotic and abiotic factors in the environment. In wheat, EW is associated with resilience to heat and drought stress, however...

    Authors: Fátima Camarillo-Castillo, Trevis D. Huggins, Suchismita Mondal, Matthew P. Reynolds, Michael Tilley and Dirk B. Hays

    Citation: Plant Methods 2021 17:58

    Content type: Research

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  4. Water content variation during plant growth is one of the most important monitoring parameters in plant studies. Conventional parameters (such as dry weight) are unreliable; thus, the development of rapid, acc...

    Authors: Yunjeong Yang, Ji Eun Kim, Hak Jin Song, Eun Bin Lee, Yong-Keun Choi, Jeong Wook Jo, Hyeon Jin Jeon, Ho Hyun Kim, Kwang Jin Kim and Hyung Joo Kim

    Citation: Plant Methods 2021 17:56

    Content type: Methodology

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  5. Water uptake is essential for seed germination. However, Gleditsia sinensis seeds have a water-impermeable seed coat, which is beneficial for its adaption to the environment, but prohibits its germination without...

    Authors: Mingwei Zhu, Song Dai, Qiuyue Ma and Shuxian Li

    Citation: Plant Methods 2021 17:55

    Content type: Research

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  6. Annotating gene structures and functions to genome assemblies is necessary to make assembly resources useful for biological inference. Gene Ontology (GO) term assignment is the most used functional annotation ...

    Authors: Kokulapalan Wimalanathan and Carolyn J. Lawrence-Dill

    Citation: Plant Methods 2021 17:54

    Content type: Software

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  7. A variety of basic and applied research programs in plant biology require the accurate and reliable determination of plant tissue cold hardiness. Over the past 50 years, the electrolyte leakage method has emer...

    Authors: Alisson P. Kovaleski and Jake J. Grossman

    Citation: Plant Methods 2021 17:53

    Content type: Methodology

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  8. Fractional vegetation cover (FVC) is an important parameter for evaluating crop-growth status. Optical remote-sensing techniques combined with the pixel dichotomy model (PDM) are widely used to estimate cropla...

    Authors: Jibo Yue, Wei Guo, Guijun Yang, Chengquan Zhou, Haikuan Feng and Hongbo Qiao

    Citation: Plant Methods 2021 17:51

    Content type: Methodology

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  9. To accurately estimate winter wheat leaf area index (LAI) using unmanned aerial vehicle (UAV) hyperspectral imagery is crucial for crop growth monitoring, fertilization management, and development of precision...

    Authors: Juanjuan Zhang, Tao Cheng, Wei Guo, Xin Xu, Hongbo Qiao, Yimin Xie and Xinming Ma

    Citation: Plant Methods 2021 17:49

    Content type: Research

    Published on:

  10. Maize (Zea mays L.) is one of the most important food sources in the world and has been one of the main targets of plant genetics and phenotypic research for centuries. Observation and analysis of various morp...

    Authors: Shuo Zhou, Xiujuan Chai, Zixuan Yang, Hongwu Wang, Chenxue Yang and Tan Sun

    Citation: Plant Methods 2021 17:48

    Content type: Software

    Published on:

  11. Circadian rhythms regulate several physiological and developmental processes of plants. Hence, the identification of genes with the underlying circadian rhythmic features is pivotal. Though computational metho...

    Authors: Prabina Kumar Meher, Ansuman Mohapatra, Subhrajit Satpathy, Anuj Sharma, Isha Saini, Sukanta Kumar Pradhan and Anil Rai

    Citation: Plant Methods 2021 17:46

    Content type: Methodology

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  12. The paper provides studies on the structure of planted small-leaved lime (Tilia cordata Mill.) in the conditions of the Bashkir Cis-Urals. This work aimed to analyze their assortment by diameters and compile appr...

    Authors: Aydar Gabdelkhakov, Zagir Rakhmatullin, Maria Martynova, Ilyas Fazlutdinov and Ilnur Mullagaleev

    Citation: Plant Methods 2021 17:45

    Content type: Research

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  13. How to control the physical damage during maize kernel harvesting is a major problem for both mechanical designers and plant breeders. A limitation of addressing this problem is lacking a reliable method for a...

    Authors: Yuan Su, Yang Xu, Tao Cui, Xiaojun Gao, Guoyi Xia, Yibo Li, Mengmeng Qiao and Yingbo Yu

    Citation: Plant Methods 2021 17:44

    Content type: Research

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  14. Syntrichia caninervis is a typical desiccation tolerant moss that is a dominant species forming biological soil crusts in the Gurbantunggut Desert. This study investigated the effect of different explants on rege...

    Authors: Xiujin Liu, Ping Zhou, Xiaoshuang Li and Daoyuan Zhang

    Citation: Plant Methods 2021 17:42

    Content type: Research

    Published on:

  15. Investigating plant mechanisms to tolerate freezing temperatures is critical to developing crops with superior cold hardiness. However, the lack of imaging methods that allow the visualization of freezing even...

    Authors: Camilo Villouta, Benjamin L. Cox, Beth Rauch, Beth Ann A. Workmaster, Kevin W. Eliceiri and Amaya Atucha

    Citation: Plant Methods 2021 17:41

    Content type: Methodology

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  16. Crop yield is dependent on climate conditions, which are becoming both more variable and extreme in some areas of the world as a consequence of global climate change. Increased precipitation and flooding event...

    Authors: Alexandra Miricescu, Tomás Byrne, Catherine M. Doorly, Carl K. Y. Ng, Susanne Barth and Emmanuelle Graciet

    Citation: Plant Methods 2021 17:40

    Content type: Methodology

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  17. X-ray computed tomography is acknowledged as a powerful tool for the study of root system architecture of plants growing in soil. In this paper, we improved the original root segmentation algorithm “Rootine” a...

    Authors: Maxime Phalempin, Eva Lippold, Doris Vetterlein and Steffen Schlüter

    Citation: Plant Methods 2021 17:39

    Content type: Methodology

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  18. Wood basic density (WBD) is one of the most crucial wood property in tree and mainly determined the end use of wood for industry. However, the measurement WBD is time- and cost-consuming, which an alternativel...

    Authors: Yanjie Li, Wenjian Liu, Ruishu Cao, Zifeng Tan, Jun Liu and Jingmin Jiang

    Citation: Plant Methods 2021 17:35

    Content type: Research

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  19. Plant traits related to nutrition have an influential role in tree growth, tree production and nutrient cycling. Therefore, the breeding program should consider the genetics of the traits. However, the measure...

    Authors: Jun Liu, Yang Sun, Wenjian Liu, Zifeng Tan, Jingmin Jiang and Yanjie Li

    Citation: Plant Methods 2021 17:33

    Content type: Research

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  20. We report a method to estimate carbon assimilation based on isotope ratio-mass spectrometry (IRMS) of 13CO2 labeled plant tissue. Photosynthetic carbon assimilation is the principal experimental observable which ...

    Authors: Matthew E. Bergman, Diego González-Cabanelas, Louwrance P. Wright, Berkley J. Walker and Michael A. Phillips

    Citation: Plant Methods 2021 17:32

    Content type: Methodology

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  21. In vitro culture of olive, as an economically valuable tree, has fundamentally a genotype-dependant low micropropagation rate which needs to be improved in already established and newly released cultivars. Var...

    Authors: Leila Mirzaei, Abbas Yadollahi, Maryam Jafarkhani Kermani, Masoud Naderpour, Ali Asghar Zeinanloo, Maryam Farsi and Dariush Davoodi

    Citation: Plant Methods 2021 17:31

    Content type: Research

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  22. K-seq, a new genotyping methodology based on the amplification of genomic regions using two steps of Klenow amplification with short oligonucleotides, followed by standard PCR and Illumina sequencing, is prese...

    Authors: Peio Ziarsolo, Tomas Hasing, Rebeca Hilario, Victor Garcia-Carpintero, Jose Blanca, Aureliano Bombarely and Joaquin Cañizares

    Citation: Plant Methods 2021 17:30

    Content type: Methodology

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  23. New cell wall imaging tools permit direct visualization of the molecular architecture of cell walls and provide detailed chemical information on wall polymers, which will aid efforts to use these polymers in m...

    Authors: Huimin Xu, Yuanyuan Zhao, Yuanzhen Suo, Yayu Guo, Yi Man, Yanping Jing, Xinqiang He and Jinxing Lin

    Citation: Plant Methods 2021 17:29

    Content type: Methodology

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  24. Wheat spike architecture is a key determinant of multiple grain yield components and detailed examination of spike morphometric traits is beneficial to explain wheat grain yield and the effects of differing ag...

    Authors: Hu Zhou, Andrew B. Riche, Malcolm J. Hawkesford, William R. Whalley, Brian S. Atkinson, Craig J. Sturrock and Sacha J. Mooney

    Citation: Plant Methods 2021 17:26

    Content type: Methodology

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  25. Floral temperature has important consequences for plant biology, and accurate temperature measurements are therefore important to plant research. Thermography, also referred to as thermal imaging, is beginning...

    Authors: Michael J. M. Harrap and Sean A. Rands

    Citation: Plant Methods 2021 17:23

    Content type: Research

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  26. The virus-induced genome editing (VIGE) system can be used to quickly identify gene functions and generate knock-out libraries as an alternative to the virus-induced gene silencing (VIGS). Although plant virus...

    Authors: Jianfeng Lei, Peihong Dai, Yue Li, Wanqi Zhang, Guantong Zhou, Chao Liu and Xiaodong Liu

    Citation: Plant Methods 2021 17:20

    Content type: Methodology

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  27. Dispensability of genes in a phylogenetic lineage, e.g. a species, genus, or higher-level clade, is gaining relevance as most genome sequencing projects move to a pangenome level. Most analyses classify genes ...

    Authors: Katharina Sielemann, Bernd Weisshaar and Boas Pucker

    Citation: Plant Methods 2021 17:18

    Content type: Methodology

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  28. Upland cotton (Gossypium hirsutum), harboring a complex allotetraploid genome, consists of A and D sub-genomes. Every gene has multiple copies with high sequence similarity that makes genetic, genomic and functio...

    Authors: Mohamed Ramadan, Muna Alariqi, Yizan Ma, Yanlong Li, Zhenping Liu, Rui Zhang, Shuangxia Jin, Ling Min and Xianlong Zhang

    Citation: Plant Methods 2021 17:16

    Content type: Methodology

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  29. Casbene synthase (CS) is responsible for the first committed step in the biosynthesis of phorbol esters (PE) in the Euphorbiaceae. PE are abundant in the seeds of the biofuel crop Jatropha curcas and its toxicity...

    Authors: Natália Pinto de Almeida, Domingos Ferreira Mélo Neto, Gabriel Reis Alves Carneiro, Andreza Raquel Barbosa de Farias, Gilberto Barbosa Domont, Francisco de Assis de Paiva Campos and Fábio César Sousa Nogueira

    Citation: Plant Methods 2021 17:15

    Content type: Research

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  30. The presence of condensed tannins (CT) in tree fodders entails a series of productive, health and ecological benefits for ruminant nutrition. Current wet analytical methods employed for full CT characterisatio...

    Authors: Jordi Ortuño, Sokratis Stergiadis, Anastasios Koidis, Jo Smith, Chris Humphrey, Lindsay Whistance and Katerina Theodoridou

    Citation: Plant Methods 2021 17:14

    Content type: Research

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  31. Paclitaxel is a well-known chemotherapeutic agent widely applied as a therapy for various types of cancers. In vitro culture of Corylus avellana has been named as a promising and low-cost strategy for paclitaxel ...

    Authors: Mina Salehi, Siamak Farhadi, Ahmad Moieni, Naser Safaie and Mohsen Hesami

    Citation: Plant Methods 2021 17:13

    Content type: Research

    Published on:

  32. Pyropia is an economically advantageous genus of red macroalgae, which has been cultivated in the coastal areas of East Asia for over 300 years. Realizing estimation of macroalgae biomass in a high-throughput way...

    Authors: Shuai Che, Guoying Du, Ning Wang, Kun He, Zhaolan Mo, Bin Sun, Yu Chen, Yifei Cao, Junhao Wang and Yunxiang Mao

    Citation: Plant Methods 2021 17:12

    Content type: Methodology

    Published on:

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