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  1. Living cells maintain and adjust structural and functional integrity by continual synthesis and degradation of metabolites and macromolecules. The maintenance and adjustment of thylakoid membrane involve turno...

    Authors: Anh Thi-Mai Banh, Björn Thiele, Antonia Chlubek, Thomas Hombach, Einhard Kleist and Shizue Matsubara
    Citation: Plant Methods 2022 18:114
  2. As one of the most widely planted fruit trees in southern China, citrus occupies an important position in the agriculture field and forestry economy in China. There are many kinds of citrus diseases. If citrus...

    Authors: Ruoli Yang, Tingjing Liao, Peirui Zhao, Wenhua Zhou, Mingfang He and Liujun Li
    Citation: Plant Methods 2022 18:113
  3. Defining interactions of bacteria in the rhizosphere (encompassing the area near and on the plant root) is important to understand how they affect plant health. Some rhizosphere bacteria, including plant growt...

    Authors: Amanda Rosier and Harsh P. Bais
    Citation: Plant Methods 2022 18:112
  4. According to the most popular definition, a biostimulant is any substance or microorganism applied to plants with the aim to enhance nutrition efficiency, abiotic stress tolerance and/or crop quality traits, r...

    Authors: Patricia Benito, Daniele Ligorio, Javier Bellón, Lynne Yenush and José M. Mulet
    Citation: Plant Methods 2022 18:111
  5. Phenomic prediction has been defined as an alternative to genomic prediction by using spectra instead of molecular markers. A reflectance spectrum provides information on the biochemical composition within a t...

    Authors: Charlotte Brault, Juliette Lazerges, Agnès Doligez, Miguel Thomas, Martin Ecarnot, Pierre Roumet, Yves Bertrand, Gilles Berger, Thierry Pons, Pierre François, Loïc Le Cunff, Patrice This and Vincent Segura
    Citation: Plant Methods 2022 18:108
  6. The allocation of non-structural carbohydrates (NSCs) plays a critical role in the physiology and metabolism of tree growth and survival defense. However, little is known about the allocation of NSC after cont...

    Authors: Yanjie Li, Honggang Sun, Thiago de Paula Protásio, Paulo Ricardo Gherardi Hein and Baoguo Du
    Citation: Plant Methods 2022 18:107
  7. Cowpea is a dryland crop with potential to improve food security in sub-Saharan Africa, where it is mostly produced and consumed. Contemporary plant improvement technologies, including genome editing, marker-a...

    Authors: Offiong Ukpong Edet and Takayoshi Ishii
    Citation: Plant Methods 2022 18:106
  8. Genome complexity is largely linked to diversification and crop innovation. Examples of regions with duplicated genes with relevant roles in agricultural traits are found in many crops. In both duplicated and ...

    Authors: Arnau Fiol, Federico Jurado-Ruiz, Elena López‑Girona and Maria José Aranzana
    Citation: Plant Methods 2022 18:105
  9. Eucommia ulmoides leaf (EUL), as a medicine and food homology plant, is a high-quality industrial raw material with great development potential for a valuable economic crop. There are many factors affecting the q...

    Authors: Lian Li, Zhi Min Li and Yuan Zhong Wang
    Citation: Plant Methods 2022 18:102
  10. As a rapid and non-destructive method, Near Infrared Spectroscopy is classically proposed to assess plant traits in many scientific fields, to observe enlarged genotype panels and to document the temporal kine...

    Authors: Héloïse Villesseche, Martin Ecarnot, Elsa Ballini, Ryad Bendoula, Nathalie Gorretta and Pierre Roumet
    Citation: Plant Methods 2022 18:100
  11. Quantification of gene expression such as RNA-Seq is a popular approach to study various biological phenomena. Despite the development of RNA-Seq library preparation methods and sequencing platforms in the las...

    Authors: Makoto Kashima, Mari Kamitani, Yasuyuki Nomura, Natsumi Mori-Moriyama, Shigeyuki Betsuyaku, Hiromi Hirata and Atsushi J. Nagano
    Citation: Plant Methods 2022 18:99
  12. Genome editing is essential for crop molecular breeding. However, gene editing in turnip (Brassica rapa var. rapa) have not been reported owing to the very low transformation efficiency.

    Authors: Yuanyuan Liu, Li Zhang, Cheng Li, Yunqiang Yang, Yuanwen Duan, Yongping Yang and Xudong Sun
    Citation: Plant Methods 2022 18:98
  13. Photosynthesis close interacts with respiration and nitrogen assimilation, which determine the photosynthetic efficiency of a leaf. Accurately quantifying the metabolic fluxes in photosynthesis, respiration an...

    Authors: Qiming Tang, Qingfeng Song, Xiaoxiang Ni, Zai Shi, Genyun Chen and Xinguang Zhu
    Citation: Plant Methods 2022 18:97
  14. Characterizing plant genetic resources and their response to the environment through accurate measurement of relevant traits is crucial to genetics and breeding. Spatial organization of the maize ear provides ...

    Authors: V. Oury, T. Leroux, O. Turc, R. Chapuis, C. Palaffre, F. Tardieu, S. Alvarez Prado, C. Welcker and S. Lacube
    Citation: Plant Methods 2022 18:96
  15. The demand for productive economic plant resources is increasing with the continued growth of the human population. Ancient Pu’er tea trees [Camellia sinensis var. assamica (J. W. Mast.) Kitam.] are an important ...

    Authors: Shuqiao Zhang, Wendou Liu, Xinmeng Cheng, Zizhi Wang, Fengjun Yuan, Wengui Wu and Shengxi Liao
    Citation: Plant Methods 2022 18:95
  16. Bulk segregant analysis (BSA) can help identify quantitative trait loci (QTLs), but this may result in substantial bycatch of functionally irrelevant genes.

    Authors: Rutger A. Vos, Catharina A. M. van der Veen-van Wijk, M. Eric Schranz, Klaas Vrieling, Peter G. L. Klinkhamer and Frederic Lens
    Citation: Plant Methods 2022 18:92
  17. Clubroot of canola (Brassica napus), caused by the soilborne pathogen Plasmodiophora brassicae, has become a serious threat to canola production in Canada. The deployment of clubroot-resistant (CR) cultivars is t...

    Authors: Heather H. Tso, Leonardo Galindo-González, Troy Locke and Stephen E. Strelkov
    Citation: Plant Methods 2022 18:91
  18. The application of autopilot technology is conductive to achieving path planning navigation and liberating labor productivity. In addition, the self-driving vehicles can drive according to the growth state of ...

    Authors: Xihuizi Liang, Bingqi Chen, Chaojie Wei and Xiongchu Zhang
    Citation: Plant Methods 2022 18:90
  19. Salicylic acid (SA) is one of the plant hormones, which plays crucial roles in signaling transduction in plant growth, disease resistance, and leaf senescence. Arabidopsis (Arabidopsis thaliana) SA 3-hydroxylase ...

    Authors: Zilin Cai, Hao Guo, Shijing Shen, Qilu Yu, Jinbin Wang, Engao Zhu, Pinghua Zhang, Lili Song, Yanjun Zhang and Kewei Zhang
    Citation: Plant Methods 2022 18:89
  20. Jubaea chilensis (Molina) Baillon, is a uniquely large palm species endemic to Chile. It is under threatened status despite its use as an ornamental species throughout the world. This research seeks to identify t...

    Authors: Eduardo Guzmán, M. Paulina Fernández, José-Antonio Alcalde, Samuel Contreras, Pasi Raumonen, Lorenzo Picco, Cristián Montalba and Cristián Tejos
    Citation: Plant Methods 2022 18:88
  21. Quercetin is one of the most important bioflavonoids having positive effects on the biological processes and human health. Typically, it is extracted from plant matrices using conventional methods such as mace...

    Authors: Veronika Pilařová, Lukáš Kuda, Hana Kočová Vlčková, Lucie Nováková, Shubhpriya Gupta, Manoj Kulkarni, František Švec, Johannes Van Staden and Karel Doležal
    Citation: Plant Methods 2022 18:87
  22. Methods to accurately quantify disease severity are fundamental to plant pathogen interaction studies. Commonly used methods include visual scoring of disease symptoms, tracking pathogen growth in planta over tim...

    Authors: Kiona Elliott, Jeffrey C. Berry, Hobin Kim and Rebecca S. Bart
    Citation: Plant Methods 2022 18:86
  23. Elaeagnus angustifolia L. is a deciduous tree in the family Elaeagnaceae. It is widely used to study abiotic stress tolerance in plants and to improve desertification-affected land because of its ability to withs...

    Authors: Yunfei Mao, Xueli Cui, Haiyan Wang, Xin Qin, Yangbo Liu, Yijun Yin, Xiafei Su, Juan Tang, Fengling Wang, Fengwang Ma, Naibin Duan, Donglin Zhang, Yanli Hu, Wenli Wang, Shaochong Wei, Xiaoliu Chen…
    Citation: Plant Methods 2022 18:84
  24. Lasers enable modification of living and non-living matter with submicron precision in a contact-free manner which has raised the interest of researchers for decades. Accordingly, laser technologies have drawn...

    Authors: Dag Heinemann, Miroslav Zabic, Mitsuhiro Terakawa and Jens Boch
    Citation: Plant Methods 2022 18:82

    The Correction to this article has been published in Plant Methods 2022 18:104

  25. Variety genuineness and purity are essential indices of maize seed quality that affect yield. However, detection methods for variety genuineness are time-consuming, expensive, require extensive training, or de...

    Authors: Keling Tu, Shaozhe Wen, Ying Cheng, Yanan Xu, Tong Pan, Haonan Hou, Riliang Gu, Jianhua Wang, Fengge Wang and Qun Sun
    Citation: Plant Methods 2022 18:81
  26. The interaction between canopy and droplets is very important in the process of crop spraying. During the actual air-assisted application process, air-mist flow inevitably disturbs the leaves before droplets r...

    Authors: Yubin Cao, Te Xi, Lujiang Xu, Wei Qiu, Hongbin Guo, Xiaolan Lv and Chao Li
    Citation: Plant Methods 2022 18:80
  27. The identification of tropical African wood species based on microscopic imagery is a challenging problem due to the heterogeneous nature of the composition of wood combined with the vast number of candidate s...

    Authors: Núbia Rosa da Silva, Victor Deklerck, Jan M. Baetens, Jan Van den Bulcke, Maaike De Ridder, Mélissa Rousseau, Odemir Martinez Bruno, Hans Beeckman, Joris Van Acker, Bernard De Baets and Jan Verwaeren
    Citation: Plant Methods 2022 18:79
  28. Many significant ecosystems, including important non-forest woody ecosystems such as the Cerrado (Brazilian savannah), are under threat from climate change, yet our understanding of how increasing temperatures...

    Authors: Georgina A. Werkmeister, David Galbraith, Emma Docherty, Camilla Silva Borges, Jairo Matos da Rocha, Paulo Alves da Silva, Beatriz Schwantes Marimon, Ben Hur Marimon-Junior, Oliver L. Phillips and Emanuel Gloor
    Citation: Plant Methods 2022 18:78
  29. The superposition of COVID-19 and climate change has brought great challenges to global food security. As a major economic crop in the world, studying its phenotype to cultivate high-quality wheat varieties is...

    Authors: Anhua Ren, Dong Jiang, Min Kang, Jie Wu, Fangcheng Xiao, Pei Hou and Xiuqing Fu
    Citation: Plant Methods 2022 18:77
  30. In India, raw peanuts are obtained by aggregators from smallholder farms in the form of whole pods and the price is based on a manual estimation of basic peanut pod and kernel characteristics. These methods of...

    Authors: Martha Domhoefer, Debarati Chakraborty, Eva Hufnagel, Joelle Claußen, Norbert Wörlein, Marijn Voorhaar, Krithika Anbazhagan, Sunita Choudhary, Janila Pasupuleti, Rekha Baddam, Jana Kholova and Stefan Gerth
    Citation: Plant Methods 2022 18:76
  31. Optical sensing solutions are being developed and adopted to classify a wide range of biological objects, including crop seeds. Performance assessment of optical classification models remains both a priority a...

    Authors: Christian Nansen, Mohammad S. Imtiaz, Mohsen B. Mesgaran and Hyoseok Lee
    Citation: Plant Methods 2022 18:74
  32. Triticum aestivum is the most important staple food grain of the world. In recent years, the outbreak of a major seed-borne disease, common bunt, in wheat resulted in reduced quality and quantity of the crop. The...

    Authors: Raghav Kataria and Rakesh Kaundal
    Citation: Plant Methods 2022 18:73
  33. Although the genome for the allotetraploid bioenergy crop switchgrass (Panicum virgatum) has been established, limitations in mutant resources have hampered in planta gene function studies toward crop optimizatio...

    Authors: Kira Tiedge, Janessa Destremps, Janet Solano-Sanchez, Magda Lisette Arce-Rodriguez and Philipp Zerbe
    Citation: Plant Methods 2022 18:71
  34. Seeds were an important medium for long-distance transmission of plant viruses. Therefore, appropriate, more sensitive methods for detecting low concentrations of virus-infected in seeds were crucial to ensure...

    Authors: Yin Xinying, Li Xin, Yang Lili, Zheng Qiuyue, Piao Yongzhe and Cao Jijuan
    Citation: Plant Methods 2022 18:70
  35. The bimolecular fluorescence complementation (BiFC) assay has emerged as one of the most popular methods for analysing protein–protein interactions (PPIs) in plant biology. This includes its increasing use as ...

    Authors: Florent Velay, Mélanie Soula, Marwa Mehrez, Clément Belbachir, Stefano D’Alessandro, Christophe Laloi, Patrice Crete and Ben Field
    Citation: Plant Methods 2022 18:69
  36. The chlorophyll content is a vital indicator for reflecting the photosynthesis ability of plants and it plays a significant role in monitoring the general health of plants. Since the chlorophyll content and th...

    Authors: YiFei Cao, Huanliang Xu, Jin Song, Yao Yang, Xiaohui Hu, Korohou Tchalla Wiyao and Zhaoyu Zhai
    Citation: Plant Methods 2022 18:67
  37. Quinoa is an increasingly popular seed crop frequently studied for its tolerance to various abiotic stresses as well as its susceptibility to heat. Estimations of quinoa pollen viability through staining metho...

    Authors: S. Elizabeth Castillo, Jose C. Tovar, Anastasia Shamin, Jorge Gutirerrez, Paige Pearson and Malia A. Gehan
    Citation: Plant Methods 2022 18:65
  38. Although quantitative single-cell analysis is frequently applied in animal systems, e.g. to identify novel drugs, similar applications on plant single cells are largely missing. We have exploited the applicabi...

    Authors: Jonathan Dawson, Saurabh Pandey, Qiuju Yu, Patrick Schaub, Florian Wüst, Amir Bahram Moradi, Oleksandr Dovzhenko, Klaus Palme and Ralf Welsch
    Citation: Plant Methods 2022 18:64

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