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  1. Content type: Methodology

    Roots are vital organs for plants, and the effective use of resources from the soil is important for yield stability. However, phenotypic variation in root traits among crop genotypes is mostly unknown and fie...

    Authors: Simon Fiil Svane, Christian Sig Jensen and Kristian Thorup-Kristensen

    Citation: Plant Methods 2019 15:26

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  2. Content type: Methodology

    We have recently developed a simple, rapid, and relatively-cheap method for matrix-assisted laser-desorption and ionisation time-of-flight mass spectroscopy (MALDI-TOF MS) sample preparation that is applicable...

    Authors: Michael A. Reeve and Kathryn M. Pollard

    Citation: Plant Methods 2019 15:25

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  3. Content type: Research

    The traditional methods for evaluating seeds are usually performed through destructive sampling followed by physical, physiological, biochemical and molecular determinations. Whilst proven to be effective, the...

    Authors: Gamal ElMasry, Nasser Mandour, Marie-Hélène Wagner, Didier Demilly, Jerome Verdier, Etienne Belin and David Rousseau

    Citation: Plant Methods 2019 15:24

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  4. Content type: Methodology

    Nowadays, there is an increasing demand for fast and reliable plant biomolecular analyses. Conventional methods for the isolation of nucleic acids are time-consuming and require multiple and often non-automata...

    Authors: Arianna Marengo, Cecilia Cagliero, Barbara Sgorbini, Jared L. Anderson, Miranda N. Emaus, Carlo Bicchi, Cinzia M. Bertea and Patrizia Rubiolo

    Citation: Plant Methods 2019 15:23

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  5. Content type: Methodology

    In the present work, we provide an account of structured illumination microscopy (SIM) imaging of fixed and immunolabeled plant probes. We take advantage of SIM, to superresolve intracellular structures at a c...

    Authors: T. Vavrdová, O. Šamajová, P. Křenek, M. Ovečka, P. Floková, R. Šnaurová, J. Šamaj and G. Komis

    Citation: Plant Methods 2019 15:22

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  6. Content type: Methodology

    Tissue culture transformation of plants has an element of art to it, with protocols passed on between labs but often not directly compared. As Medicago truncatula has become popular as a model system for legumes,...

    Authors: Li Wen, Yuanling Chen, Elise Schnabel, Ashley Crook and Julia Frugoli

    Citation: Plant Methods 2019 15:20

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  7. Content type: Methodology

    Chlorophyll fluorescence analysis is one of the non-invasive techniques widely used to detect and quantify the stress-induced changes in the photosynthetic apparatus. Quantitative information is obtained as a ...

    Authors: Joanna Sekulska-Nalewajko, Andrzej Kornaś, Jarosław Gocławski, Zbigniew Miszalski and Elżbieta Kuźniak

    Citation: Plant Methods 2019 15:18

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  8. Content type: Research

    Aboveground biomass (AGB) is a widely used agronomic parameter for characterizing crop growth status and predicting grain yield. The rapid and accurate estimation of AGB in a non-destructive way is useful for ...

    Authors: Ning Lu, Jie Zhou, Zixu Han, Dong Li, Qiang Cao, Xia Yao, Yongchao Tian, Yan Zhu, Weixing Cao and Tao Cheng

    Citation: Plant Methods 2019 15:17

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  9. Content type: Methodology

    Plant responses triggered upon detection of an invading pathogen include the generation of a number of mobile signals that travel to distant tissues and determine an increased resistance in distal, uninfected ...

    Authors: José S. Rufián, Javier Rueda-Blanco, Carmen R. Beuzón and Javier Ruiz-Albert

    Citation: Plant Methods 2019 15:16

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  10. Content type: Research

    Crop emergence and canopy cover are important physiological traits for potato (Solanum tuberosum L.) cultivar evaluation and nutrients management. They play important roles in variety screening, field management ...

    Authors: Bo Li, Xiangming Xu, Jiwan Han, Li Zhang, Chunsong Bian, Liping Jin and Jiangang Liu

    Citation: Plant Methods 2019 15:15

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  11. Content type: Research

    The selection of hybrids is an essential step in maize breeding. However, evaluating a large number of hybrids in field trials can be extremely costly. However, genomic models can be used to predict the expect...

    Authors: Filipe Couto Alves, Ítalo Stefanine Correa Granato, Giovanni Galli, Danilo Hottis Lyra, Roberto Fritsche-Neto and Gustavo de los Campos

    Citation: Plant Methods 2019 15:14

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  12. Content type: Methodology

    Recent advances in high throughput phenotyping have made it possible to collect large datasets following plant growth and development over time, and those in machine learning have made inferring phenotypic pla...

    Authors: Raghav Khanna, Lukas Schmid, Achim Walter, Juan Nieto, Roland Siegwart and Frank Liebisch

    Citation: Plant Methods 2019 15:13

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  13. Content type: Research

    Maize (Zea mays L.) is the third most consumed grain in the world and improving maize yield is of great importance of the world food security, especially under global climate change and more frequent severe droug...

    Authors: Yanjun Su, Fangfang Wu, Zurui Ao, Shichao Jin, Feng Qin, Boxin Liu, Shuxin Pang, Lingli Liu and Qinghua Guo

    Citation: Plant Methods 2019 15:11

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  14. Content type: Research

    Above-ground biomass (AGB) is a basic agronomic parameter for field investigation and is frequently used to indicate crop growth status, the effects of agricultural management practices, and the ability to seq...

    Authors: Liang Han, Guijun Yang, Huayang Dai, Bo Xu, Hao Yang, Haikuan Feng, Zhenhai Li and Xiaodong Yang

    Citation: Plant Methods 2019 15:10

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  15. Content type: Research

    Bacterial volatiles promote plant growth and elicit immunity responses in plants grown in two-compartment Petri dishes. Due to the limitations of bacterial volatile compound (BVC) treatments such as their high...

    Authors: Geun Cheol Song, Myoungjoo Riu and Choong-Min Ryu

    Citation: Plant Methods 2019 15:9

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  16. Content type: Research

    Plant root apex is the major part to direct the root growth and development by responding to various signals/cues from internal and soil environments. To study and understand root system biology particularly a...

    Authors: Lei Zhang, Li-Na Qin, Zi-Rui Zeng, Chang-Zheng Wu, Yuan-Yong Gong, Lai-Hua Liu and Feng-Qiu Cao

    Citation: Plant Methods 2019 15:8

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  17. Content type: Research

    Loquat (Eriobotrya japonica) is a subtropical tree bearing fruit that ripens during late spring and early summer, which is the off-season for fruit production. The specific flowering habit of loquat, which starts...

    Authors: Wenbing Su, Yuan Yuan, Ling Zhang, Yuanyuan Jiang, Xiaoqing Gan, Yunlu Bai, Jiangrong Peng, Jincheng Wu, Yuexue Liu and Shunquan Lin

    Citation: Plant Methods 2019 15:7

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  18. Content type: Methodology

    Remote monitoring of plants using hyperspectral imaging has become an important tool for the study of plant growth, development, and physiology. Many applications are oriented towards use in field environments...

    Authors: Max R. Lien, Richard J. Barker, Zhiwei Ye, Matthew H. Westphall, Ruohan Gao, Aditya Singh, Simon Gilroy and Philip A. Townsend

    Citation: Plant Methods 2019 15:6

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  19. Content type: Research

    Open tissue culture technique could be simplified by using different bacteriostatic agents. There is a great difference in the bacteriostatic effects of different antimicrobial agents on various explants. Howe...

    Authors: Liejian Huang, Hong Wang, Muhammad Qasim Shahid and Bingshan Zeng

    Citation: Plant Methods 2019 15:5

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  20. Content type: Methodology

    The use of spectral imaging within the plant phenotyping and breeding community has been increasing due its utility as a non-invasive diagnostic tool. However, there is a lack of imaging systems targeted speci...

    Authors: Charles Veys, Fokion Chatziavgerinos, Ali AlSuwaidi, James Hibbert, Mark Hansen, Gytis Bernotas, Melvyn Smith, Hujun Yin, Stephen Rolfe and Bruce Grieve

    Citation: Plant Methods 2019 15:4

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  21. Content type: Methodology

    Global warming is going to affect both agricultural production and carbon storage in soil worldwide. Given the complexity of the soil-plant-atmosphere continuum, in situ experiments of climate warming are nece...

    Authors: Christophe Moni, Hanna Silvennoinen, Bruce A. Kimball, Erling Fjelldal, Marius Brenden, Ingunn Burud, Andreas Flø and Daniel P. Rasse

    Citation: Plant Methods 2019 15:3

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  22. Content type: Software

    The development of leaf area is one of the fundamental variables to quantify plant growth and physiological function and is therefore widely used to characterize genotypes and their interaction with the enviro...

    Authors: Mark Müller-Linow, Jens Wilhelm, Christoph Briese, Tobias Wojciechowski, Ulrich Schurr and Fabio Fiorani

    Citation: Plant Methods 2019 15:2

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  23. Content type: Methodology

    Cutin is a complex, highly cross-linked polyester consisting of hydroxylated and epoxidated acyl lipid monomers. Because of the complexity of the polymer it has been difficult to define the chemical architectu...

    Authors: Rupam Kumar Bhunia, Lucas J. Showman, Adarsh Jose and Basil J. Nikolau

    Citation: Plant Methods 2018 14:117

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  24. Content type: Research

    Imbibition (uptake of water by a dry seed) initiates the germination process. An automated method for quantifying imbibition would enable research on the genetic elements that influence the underlying hydrauli...

    Authors: Nathan D. Miller, Scott C. Stelpflug, Shawn M. Kaeppler and Edgar P. Spalding

    Citation: Plant Methods 2018 14:115

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  25. Content type: Methodology

    Plant roots release a variety of organic compounds into the soil which alter the physical, chemical and biological properties of the rhizosphere. Root exudates are technically challenging to measure in soil be...

    Authors: Akitomo Kawasaki, Shoko Okada, Chunyan Zhang, Emmanuel Delhaize, Ulrike Mathesius, Alan E. Richardson, Michelle Watt, Matthew Gilliham and Peter R. Ryan

    Citation: Plant Methods 2018 14:114

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  26. Content type: Research

    There is a growing interest in the role of chromatin in acquiring and maintaining cell identity. Despite the ever-growing availability of genome-wide gene expression data, understanding how transcription progr...

    Authors: Miriam Tannenbaum, Avital Sarusi-Portuguez, Ronen Krispil, Michal Schwartz, Olga Loza, Jennifer I. C. Benichou, Assaf Mosquna and Ofir Hakim

    Citation: Plant Methods 2018 14:113

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  27. Content type: Methodology

    The mucilage is a model to study the polysaccharide biosynthesis since it is produced in large amounts and composed of complex polymers. In addition, it is of great economic interest for its technical and nutr...

    Authors: Fabien Miart, Jean-Xavier Fontaine, Christophe Pineau, Hervé Demailly, Brigitte Thomasset, Olivier Van Wuytswinkel, Karine Pageau and François Mesnard

    Citation: Plant Methods 2018 14:112

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  28. Content type: Methodology

    Genome editing of monocot plants can be accomplished by using the components of the CRISPR/Cas9 (clustered regularly interspaced short palindromic repeat/CRISPR associated Cas9) technology specifically optimiz...

    Authors: Sebastian Gasparis, Maciej Kała, Mateusz Przyborowski, Leszek A. Łyżnik, Wacław Orczyk and Anna Nadolska-Orczyk

    Citation: Plant Methods 2018 14:111

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  29. Content type: Research

    Real-time quantitative PCR has been widely used as the most reliable method to measure gene expression, due to its high accuracy and specificity. Wild barley (Hordeum brevisubulatum (Trin.) Link) is a wild relati...

    Authors: Lili Zhang, Qike Zhang, Ying Jiang, Yang Li, Haiwen Zhang and Ruifen Li

    Citation: Plant Methods 2018 14:110

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  30. Content type: Methodology

    Climate change represents a grand challenge for agricultural productivity. Understanding complex plant traits such as stress tolerance, disease resistance or crop yield is thus essential for breeding and the d...

    Authors: Werner Jud, J. Barbro Winkler, Bishu Niederbacher, Simon Niederbacher and Jörg-Peter Schnitzler

    Citation: Plant Methods 2018 14:109

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  31. Content type: Methodology

    The phenological development of the maize crop from emergence through flowering to maturity, usually expressed as a rate (i.e. 1/duration), is largely controlled by temperature in the tropics. Maize plant phen...

    Authors: Henri E. Z. Tonnang, Dan Makumbi and Peter Craufurd

    Citation: Plant Methods 2018 14:108

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  32. Content type: Methodology

    Protein N-glycosylation is initiated within the endoplasmic reticulum through the synthesis of a lipid-linked oligosaccharides (LLO) precursor. This precursor is then transferred en bloc on neo-synthesized protei...

    Authors: Pierre-Louis Lucas, Rodolphe Dumontier, Corinne Loutelier-Bourhis, Alain Mareck, Carlos Afonso, Patrice Lerouge, Narimane Mati-Baouche and Muriel Bardor

    Citation: Plant Methods 2018 14:107

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  33. Content type: Correction

    In the original version of this article [1], the spelling of the tenth author name, Moakai Yan, was incorrect. The corrected name is given in this correction article.

    Authors: S. V. G. N. Priyadarshani, Bingyan Hu, Weimin Li, Hina Ali, Haifeng Jia, Lihua Zhao, Simon Peter Ojolo, Syed Muhammad Azam, Junjie Xiong, Maokai Yan, Zia ur Rahman, Qingsong Wu and Yuan Qin

    Citation: Plant Methods 2018 14:106

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    The original article was published in Plant Methods 2018 14:95

  34. Content type: Methodology

    Ability to measure ethylene is an important aspect of postharvest management, as knowledge of endogenous ethylene production is used in assessing physiological status, while response of crops to exogenous ethy...

    Authors: Sunny George Gwanpua, Abdul Jabbar, Jeritah Tongonya, Sue Nicholson and Andrew R. East

    Citation: Plant Methods 2018 14:105

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  35. Content type: Methodology

    Abscisic acid (ABA) functions as a stress phytohormone in many growth and developmental processes in plants. The ultra-sensitive determination of ABA would help to better understand its vital roles and action ...

    Authors: Yi Su, Wei Li, Zhigang Huang, Ruozhong Wang, Weigui Luo, Qing Liu, Jianhua Tong and Langtao Xiao

    Citation: Plant Methods 2018 14:104

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  36. Content type: Research

    Fungal pathogens like Fusarium graminearum can cause severe yield losses and mycotoxin contamination of food and feed worldwide. We recently showed its ability to systemically colonize wheat via root infection. H...

    Authors: Dhaka Ram Bhandari, Qing Wang, Bin Li, Wolfgang Friedt, Andreas Römpp, Bernhard Spengler and Sven Gottwald

    Citation: Plant Methods 2018 14:103

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  37. Content type: Research

    To investigate plant hybrid sterility, we studied interspecific hybrids of two cultivated rice species, Asian rice (Oryza sativa) and African rice (O. glaberrima). Male gametes of these hybrids display complete s...

    Authors: Yoshitaka Kanaoka, Daichi Kuniyoshi, Eri Inada, Yohei Koide, Yoshihiro Okamoto, Hideshi Yasui and Yuji Kishima

    Citation: Plant Methods 2018 14:102

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  38. Content type: Research

    The number of cultivated wheat seedlings per unit area allows calculation of plant density. Wheat seedling density provides emergence data and this is useful for improving crop management. The number of wheat ...

    Authors: Tao Liu, Tianle Yang, Chunyan Li, Rui Li, Wei Wu, Xiaochun Zhong, Chengming Sun and Wenshan Guo

    Citation: Plant Methods 2018 14:101

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  39. Content type: Methodology

    Field phenotyping by remote sensing has received increased interest in recent years with the possibility of achieving high-throughput analysis of crop fields. Along with the various technological developments,...

    Authors: Md Mehedi Hasan, Joshua P. Chopin, Hamid Laga and Stanley J. Miklavcic

    Citation: Plant Methods 2018 14:100

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    The Correction to this article has been published in Plant Methods 2019 15:27

  40. Content type: Methodology

    Leaf cellular architecture plays an important role in setting limits for carbon assimilation and, thus, photosynthetic performance. However, the low density, fine structure, and sensitivity to desiccation of p...

    Authors: Andrew W. Mathers, Christopher Hepworth, Alice L. Baillie, Jen Sloan, Hannah Jones, Marjorie Lundgren, Andrew J. Fleming, Sacha J. Mooney and Craig J. Sturrock

    Citation: Plant Methods 2018 14:99

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  41. Content type: Research

    Expression of economically relevant proteins in alternative expression platforms, especially plant expression platforms, has gained significant interest in recent years. A special interest in working with plan...

    Authors: Israel A. Ramírez-Alanis, Justin B. Renaud, Silverio García-Lara, Rima Menassa and Guy A. Cardineau

    Citation: Plant Methods 2018 14:98

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  42. Content type: Research

    Improving plant water use efficiency (WUE) is a major target for improving crop yield resilience to adverse climate change. Identifying genetic variation in WUE usually relies on instantaneous measurements of ...

    Authors: Iván Jauregui, Shane A. Rothwell, Samuel H. Taylor, Martin A. J. Parry, Elizabete Carmo-Silva and Ian C. Dodd

    Citation: Plant Methods 2018 14:97

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  43. Content type: Methodology

    The clear visualization of 3D organization at the cellular level in plant tissues is needed to fully understand plant development processes. Imaging tools allow the visualization of the main fluorophores and i...

    Authors: Charlotte Bureau, Nadège Lanau, Mathieu Ingouff, Boukhaddaoui Hassan, Anne-Cécile Meunier, Fanchon Divol, Rosie Sevilla, Delphine Mieulet, Anne Dievart and Christophe Périn

    Citation: Plant Methods 2018 14:96

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  44. Content type: Research

    An efficient transformation protocol is a primary requisite to study and utilize the genetic potential of any plant species. A quick transformation system is also crucial for the functional analysis of genes a...

    Authors: S. V. G. N. Priyadarshani, Bingyan Hu, Weimin Li, Hina Ali, Haifeng Jia, Lihua Zhao, Simon Peter Ojolo, Syed Muhammad Azam, Junjie Xiong, Maokai Yan, Zia ur Rahman, Qingsong Wu and Yuan Qin

    Citation: Plant Methods 2018 14:95

    Published on:

    The Correction to this article has been published in Plant Methods 2018 14:106

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