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  1. Content type: Methodology

    Accurate estimation of heading date of paddy rice greatly helps the breeders to understand the adaptability of different crop varieties in a given location. The heading date also plays a vital role in determin...

    Authors: Sai Vikas Desai, Vineeth N. Balasubramanian, Tokihiro Fukatsu, Seishi Ninomiya and Wei Guo

    Citation: Plant Methods 2019 15:76

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  2. Content type: Research

    Partial and full root-zone drought stresses are two widely used methods to induce soil drying in plant container-culture experiments. Two methods might lead to different observational results in plant water re...

    Authors: Asfa Batool, Zheng-Guo Cheng, Nudrat Aisha Akram, Guang-Chao Lv, Jun-Lan Xiong, Ying Zhu, Muhammad Ashraf and You-Cai Xiong

    Citation: Plant Methods 2019 15:75

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  3. Content type: Methodology

    Green plants comprise two lineages: (1) the streptophytes that colonised land and (2) the chlorophytes that have adaptations to land but remained mostly aquatic. To better understand what made streptophytes so...

    Authors: Lenka Caisová and Timothy O. Jobe

    Citation: Plant Methods 2019 15:74

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  4. Content type: Research

    In-field measurement of yield and growth rate in pasture species is imprecise and costly, limiting scientific and commercial application. Our study proposed a LiDAR-based mobile platform for non-invasive veget...

    Authors: Kioumars Ghamkhar, Kenji Irie, Michael Hagedorn, Jeffrey Hsiao, Jaco Fourie, Steve Gebbie, Valerio Hoyos-Villegas, Richard George, Alan Stewart, Courtney Inch, Armin Werner and Brent Barrett

    Citation: Plant Methods 2019 15:72

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  5. Content type: Methodology

    Clearing methods allow relatively quick processing of plant material and examination of cellular structures by rendering tissues and organs translucent. They have been adapted for plant embryology, primarily t...

    Authors: Monika Kwiatkowska, Dariusz Kadłuczka, Maria Wędzony, Beata Dedicova and Ewa Grzebelus

    Citation: Plant Methods 2019 15:71

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  6. Content type: Methodology

    A suitable reference gene is an important prerequisite for guarantying accurate and reliable results in quantitative real-time PCR (qRT-PCR) analyses. However, there is no absolute universality in reference ge...

    Authors: Canbin Chen, Jingyu Wu, Qingzhu Hua, Noemi Tel-Zur, Fangfang Xie, Zhike Zhang, Jianye Chen, Rong Zhang, Guibing Hu, Jietang Zhao and Yonghua Qin

    Citation: Plant Methods 2019 15:70

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  7. Content type: Research

    Development of oligonucleotide probes facilitates chromosome identification via fluorescence in situ hybridization (FISH) in many organisms.

    Authors: Pei Du, Caihong Cui, Hua Liu, Liuyang Fu, Lina Li, Xiaodong Dai, Li Qin, Siyu Wang, Suoyi Han, Jing Xu, Bing Liu, Bingyan Huang, Fengshou Tang, Wenzhao Dong, Zengjun Qi and Xinyou Zhang

    Citation: Plant Methods 2019 15:69

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  8. Content type: Research

    The stomata of plants mainly regulate gas exchange and water dispersion between the interior and external environments of plants and play a major role in the plants’ health. The existing methods of stomata seg...

    Authors: Kexin Li, Jianping Huang, Wenlong Song, Jingtao Wang, Shuai Lv and Xiuwei Wang

    Citation: Plant Methods 2019 15:67

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  9. Content type: Research

    Hyperspectral reflectance data in the visible, near infrared and shortwave infrared range (VIS–NIR–SWIR, 400–2500 nm) are commonly used to nondestructively measure plant leaf properties. We investigated the us...

    Authors: Yufeng Ge, Abbas Atefi, Huichun Zhang, Chenyong Miao, Raghuprakash Kastoori Ramamurthy, Brandi Sigmon, Jinliang Yang and James C. Schnable

    Citation: Plant Methods 2019 15:66

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  10. Content type: Research

    Polygonatum cyrtonema Hua (P. cyrtonema) is one of the most important herbs in traditional Chinese medicine. Polysaccharides in P. cyrtonema plants comprise a class of important secondary metabolites and exhibit ...

    Authors: Chenkai Wang, Daiyin Peng, Jinhang Zhu, Derui Zhao, Yuanyuan Shi, Shengxiang Zhang, Kelong Ma, Jiawen Wu and Luqi Huang

    Citation: Plant Methods 2019 15:65

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  11. Content type: Research

    The quality of forage plants is a crucial component of animal performance and a limiting factor in pasture based production systems. Key forage attributes that may require improvement include the sugar, lipid,...

    Authors: Paul R. Shorten, Shane R. Leath, Jana Schmidt and Kioumars Ghamkhar

    Citation: Plant Methods 2019 15:63

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  12. Content type: Methodology

    Woody tropical plants contain high levels of complex organic compounds that inhibit the chemical procedures needed to extract RNA or DNA, thus compromising downstream applications such as RNA sequencing and an...

    Authors: François F. Barbier, Tinashe G. Chabikwa, Muhammad U. Ahsan, Stacey E. Cook, Rosanna Powell, Milos Tanurdzic and Christine A. Beveridge

    Citation: Plant Methods 2019 15:62

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  13. Content type: Research

    Standard strategies to identify genomic regions involved in a specific trait variation are often limited by time and resource consuming genotyping methods. Other limiting pre-requisites are the phenotyping of ...

    Authors: Charlotte Prodhomme, Danny Esselink, Theo Borm, Richard G. F. Visser, Herman J. van Eck and Jack H. Vossen

    Citation: Plant Methods 2019 15:60

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  14. Content type: Methodology

    Root-zone environment is considered difficult to analyze, particularly in interpreting interactions between environment and plant. Closed-loop soilless cultures have been introduced to prevent environmental po...

    Authors: Taewon Moon, Tae In Ahn and Jung Eek Son

    Citation: Plant Methods 2019 15:59

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  15. Content type: Research

    Vectors based on plant viruses are important tools for functional genomics, cellular biology, plant genome engineering and molecular farming. We previously reported on the construction of PepGFP2a, a viral vec...

    Authors: Fabiola Ruiz-Ramón, Raquel N. Sempere, Eduardo Méndez-López, M. Amelia Sánchez-Pina and Miguel A. Aranda

    Citation: Plant Methods 2019 15:58

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  16. Content type: Research

    With environmental deterioration, natural resource scarcity, and rapid population growth, mankind is facing severe global food security problems. To meet future needs, it is necessary to accelerate progress in...

    Authors: Liang Han, Guijun Yang, Huayang Dai, Hao Yang, Bo Xu, Heli Li, Huiling Long, Zhenhai Li, Xiaodong Yang and Chunjiang Zhao

    Citation: Plant Methods 2019 15:57

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  17. Content type: Methodology

    Soybean mosaic virus (SMV), a Potyvirus, is the most prevalent viral pathogen of soybean that causes severe yield and seed quality reductions in world soybean production. So far, multiple resistance loci for diff...

    Authors: Hua Jiang, Kai Li and Junyi Gai

    Citation: Plant Methods 2019 15:56

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  18. Content type: Research

    The advances of hyperspectral technology provide a new analytic means to decrease the gap of phenomics and genomics caused by the fast development of plant genomics with the next generation sequencing technolo...

    Authors: Dawei Sun, Haiyan Cen, Haiyong Weng, Liang Wan, Alwaseela Abdalla, Ahmed Islam El-Manawy, Yueming Zhu, Nan Zhao, Haowei Fu, Juan Tang, Xiaolong Li, Hongkun Zheng, Qingyao Shu, Fei Liu and Yong He

    Citation: Plant Methods 2019 15:54

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  19. Content type: Research

    Tanshinones are diterpenoid compounds that are used to treat cardiovascular diseases. As current extraction methods for tanshinones are inefficient, there is a pressing need to improve the production of these ...

    Authors: Tao Wei, Yonghong Gao, Kejun Deng, Lipeng Zhang, Meiling Yang, Xiaopei Liu, Caiyan Qi, Chunguo Wang, Wenqin Song, Yong Zhang and Chengbin Chen

    Citation: Plant Methods 2019 15:53

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  20. Content type: Correction

    After the publication of our article [1], it was brought to our attention that in six places in the article we omitted to use quotation marks to show where the text has been directly used from the cited refere...

    Authors: R. Makanza, M. Zaman-Allah, J. E. Cairns, J. Eyre, J. Burgueño, Ángela Pacheco, C. Diepenbrock, C. Magorokosho, A. Tarekegne, M. Olsen and B. M. Prasanna

    Citation: Plant Methods 2019 15:52

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    The original article was published in Plant Methods 2018 14:49

  21. Content type: Research

    A robust circadian clock has been implicated in plant resilience, resource-use efficiency, competitive growth and yield. A huge number of physiological processes are under circadian control in plants including...

    Authors: Hannah Rees, Susan Duncan, Peter Gould, Rachel Wells, Mark Greenwood, Thomas Brabbs and Anthony Hall

    Citation: Plant Methods 2019 15:51

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  22. Content type: Research

    Plant roots are complex, three-dimensional structures that play a central role in anchorage, water and nutrient acquisition, storage and interaction with rhizosphere microbes. Studying the development of the p...

    Authors: Diego D. J. Corona-Lopez, Sarah Sommer, Stephen A. Rolfe, Frank Podd and Bruce D. Grieve

    Citation: Plant Methods 2019 15:49

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  23. Content type: Methodology

    The outcrossing rate is a key determinant of the population-genetic structure of species and their long-term evolutionary trajectories. However, determining the outcrossing rate using current methods based on ...

    Authors: Friederike Jantzen, Natalia Wozniak, Christian Kappel, Adrien Sicard and Michael Lenhard

    Citation: Plant Methods 2019 15:47

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  24. Content type: Methodology

    Methods used to quantify protein from biological samples are often inaccurate with significant variability that requires care to minimize. The errors result from losses during protein preparation and purificat...

    Authors: Shrikaar Kambhampati, Jia Li, Bradley S. Evans and Doug K. Allen

    Citation: Plant Methods 2019 15:46

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  25. Content type: Research

    Gene editing using CRISPR/Cas9 is a simple and powerful tool for elucidating genetic controls and for crop improvement and its use has been reported in a growing number of important food crops, including recen...

    Authors: Fiona M. Wilson, Kate Harrison, Andrew D. Armitage, Andrew J. Simkin and Richard J. Harrison

    Citation: Plant Methods 2019 15:45

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  26. Content type: Methodology

    With the introduction of high-throughput multisensory imaging platforms, the automatization of multimodal image analysis has become the focus of quantitative plant research. Due to a number of natural and tech...

    Authors: Michael Henke, Astrid Junker, Kerstin Neumann, Thomas Altmann and Evgeny Gladilin

    Citation: Plant Methods 2019 15:44

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  27. Content type: Research

    Efficient seed germination is a crucial task at the beginning of crop cultivation. Although boundaries of environmental parameters that should be maintained are well studied, fine-tuning can significantly impr...

    Authors: Artyom Nikitin, Ilia Fastovets, Dmitrii Shadrin, Mariia Pukalchik and Ivan Oseledets

    Citation: Plant Methods 2019 15:43

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  28. Content type: Methodology

    Many agronomic traits have been bred into modern wheat varieties, but wheat (Triticum aestivum L.) continues to be vulnerable to heat stress, with high night-time temperature (HNT) stress shown to have large nega...

    Authors: Nathan T. Hein, Dan Wagner, Raju Bheemanahalli, David Šebela, Carlos Bustamante, Anuj Chiluwal, Mitchell L. Neilsen and S. V. Krishna Jagadish

    Citation: Plant Methods 2019 15:41

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  29. Content type: Methodology

    Glandular trichomes found in vascular plants are called natural cell factories because they synthesize and store secondary metabolites in glandular cells. To systematically understand the metabolic processes i...

    Authors: Ayako Takemori, Taiken Nakashima, Hisashi Ômura, Yuki Tanaka, Keisuke Nakata, Hiroshi Nonami and Nobuaki Takemori

    Citation: Plant Methods 2019 15:40

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  30. Content type: Research

    Guard cells perceive external and internal stimuli and regulate stomatal conductance in plants. With the use of gas exchange analyzers, time-resolved stomatal conductance responses to light intensity, [CO2] conce...

    Authors: Paulo H. O. Ceciliato, Jingbo Zhang, Qing Liu, Xin Shen, Honghong Hu, Chen Liu, Anton R. Schäffner and Julian I. Schroeder

    Citation: Plant Methods 2019 15:38

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  31. Content type: Research

    Plant height is an important selection target since it is associated with yield potential, stability and particularly with lodging resistance in various environments. Rapid and cost-effective estimation of pla...

    Authors: Muhammad Adeel Hassan, Mengjiao Yang, Luping Fu, Awais Rasheed, Bangyou Zheng, Xianchun Xia, Yonggui Xiao and Zhonghu He

    Citation: Plant Methods 2019 15:37

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  32. Content type: Research

    As strawberries are susceptible to somaclonal variation when propagated by tissue culture techniques, it is challenging to obtain the true-to-type plants necessary for continuous production of fruits of stable...

    Authors: Aung Htay Naing, Si Hyun Kim, Mi Young Chung, Soon Ki Park and Chang Kil Kim

    Citation: Plant Methods 2019 15:36

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  33. Content type: Research

    Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) is a rapid and sensitive approach to identify miRNA and protein-coding gene expression in plants. However, because of the specially design...

    Authors: Fang Tang, Liwei Chu, Wenbo Shu, Xuejiao He, Lijuan Wang and Mengzhu Lu

    Citation: Plant Methods 2019 15:35

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  34. Content type: Methodology

    Modern imaging approaches enable the acquisition of 3D and 4D datasets capturing plant organ development at cellular resolution. Computational analyses of these data enable the digitization and analysis of ind...

    Authors: Thomas Montenegro-Johnson, Soeren Strauss, Matthew D. B. Jackson, Liam Walker, Richard S. Smith and George W. Bassel

    Citation: Plant Methods 2019 15:33

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  35. Content type: Research

    Unmanned aerial vehicle (UAV)-based remote sensing provides a flexible, low-cost, and efficient approach to monitor crop growth status at fine spatial and temporal resolutions, and has a high potential to acce...

    Authors: Haiyan Cen, Liang Wan, Jiangpeng Zhu, Yijian Li, Xiaoran Li, Yueming Zhu, Haiyong Weng, Weikang Wu, Wenxin Yin, Chi Xu, Yidan Bao, Lei Feng, Jianyao Shou and Yong He

    Citation: Plant Methods 2019 15:32

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  36. Content type: Research

    Reverse transcription quantitative real-time PCR (RT-qPCR) is a widely used approach for investigating gene expression levels in plants because of its high reproducibility, sensitivity, accuracy and rapidness....

    Authors: Huapeng Sun, Xuefei Jiang, Mengli Sun, Hanqing Cong and Fei Qiao

    Citation: Plant Methods 2019 15:31

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  37. Content type: Research

    Rice canopy changes are associated with changes in the red light (R), green light (G), and blue light (B) value parameters of digital images. To rapidly diagnose the responses of rice to nitrogen (N) fertilize...

    Authors: Kailou Liu, Yazhen Li, Tianfu Han, Xichu Yu, Huicai Ye, Huiwen Hu and Zhihua Hu

    Citation: Plant Methods 2019 15:28

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