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  1. With the rise of artificial intelligence, deep learning is gradually applied to the field of agriculture and plant science. However, the excellent performance of deep learning needs to be established on massiv...

    Authors: Jiachen Yang, Xiaolan Guo, Yang Li, Francesco Marinello, Sezai Ercisli and Zhuo Zhang
    Citation: Plant Methods 2022 18:28
  2. Faba bean is an important legume crop in the world. Plant height and yield are important traits for crop improvement. The traditional plant height and yield measurement are labor intensive and time consuming. ...

    Authors: Yishan Ji, Zhen Chen, Qian Cheng, Rong Liu, Mengwei Li, Xin Yan, Guan Li, Dong Wang, Li Fu, Yu Ma, Xiuliang Jin, Xuxiao Zong and Tao Yang
    Citation: Plant Methods 2022 18:26
  3. Although there have been numerous studies describing plant growth systems for root exudate collection, a common limitation is that these systems require disruption of the plant root system to facilitate exudat...

    Authors: Martha G. Lopez-Guerrero, Peng Wang, Felicia Phares, Daniel P. Schachtman, Sophie Alvarez and Karin van Dijk
    Citation: Plant Methods 2022 18:25
  4. Grafting is a technique widely used in horticulture that also has been applied in agriculture. In plant physiology, grafting facilitates the elucidation of mechanisms underlying growth and developmental proces...

    Authors: L. Vanderstraeten, R. Sanchez-Muñoz, T. Depaepe, F. Auwelaert and D. Van Der Straeten
    Citation: Plant Methods 2022 18:24
  5. Blue Native polyacrylamide gel electrophoresis (BN PAGE) followed by denaturing PAGE is a widely used, convenient and time efficient method to separate thylakoid complexes and study their composition, abundanc...

    Authors: Éva Sárvári, Gabriella Gellén, Máté Sági-Kazár, Gitta Schlosser, Katalin Solymosi and Ádám Solti
    Citation: Plant Methods 2022 18:23
  6. Although the spectrum of effective methods and techniques that allow determination of inorganic or total phosphorus is impressive, more precise analysis of these substances in plant tissues is not a routine or...

    Authors: Dorota Wieczorek, Beata Żyszka-Haberecht, Anna Kafka and Jacek Lipok
    Citation: Plant Methods 2022 18:22
  7. In recent years, there has been an increase of interest in plant behaviour as represented by growth-driven responses. These are generally classified into nastic (internally driven) and tropic (environmentally ...

    Authors: Gabriella E. C. Gall, Talmo D. Pereira, Alex Jordan and Yasmine Meroz
    Citation: Plant Methods 2022 18:21
  8. The application of ribo nucleic acids for molecular studies requires high integrity and quality of extracted total RNA samples. In addition, the need to transfer RNA samples at room temperature without special...

    Authors: Leila Mirzaei, Abbas Yadollahi, Maryam Jafarkhani Kermani, Masoud Naderpour and Ali Asghar Zeinanloo
    Citation: Plant Methods 2022 18:19
  9. Some plastid-derived metabolites can control nuclear gene expression, chloroplast biogenesis, and chlorophyll biosynthesis. For example, norflurazon (NFZ) induced inhibition of carotenoid biosynthesis in leave...

    Authors: N. Dhami, B. J. Pogson, D. T. Tissue and C. I. Cazzonelli
    Citation: Plant Methods 2022 18:18
  10. Layered double hydroxide lactate nanosheets (LDH-lactate-NS) are powerful carriers for delivering macro-molecules into intact plant cells. In the past few years, some studies have been carried out on DNA/RNA t...

    Authors: Hongyang Wu, He Zhang, Xinyu Li, Yu Zhang, Jiankun Wang, Qiang Wang and Yinglang Wan
    Citation: Plant Methods 2022 18:17
  11. RNA live-cell imaging systems have been used to visualize subcellular mRNA distribution in living cells. The RNA-binding protein (RBP)-based RNA imaging system exploits specific RBP and the corresponding RNA r...

    Authors: Nien-Chen Huang, Kai-Ren Luo and Tien-Shin Yu
    Citation: Plant Methods 2022 18:15
  12. Developing a systematic phenotypic data analysis pipeline, creating enhanced visualizations, and interpreting the results is crucial to extract meaningful insights from data in making better breeding decisions...

    Authors: Waseem Hussain, Mahender Anumalla, Margaret Catolos, Apurva Khanna, Ma. Teresa Sta. Cruz, Joie Ramos and Sankalp Bhosale
    Citation: Plant Methods 2022 18:14
  13. Precision agriculture techniques are widely used to optimize fertilizer and soil applications. Furthermore, these techniques could also be combined with new statistical tools to assist in phenotyping in breedi...

    Authors: Dthenifer Cordeiro Santana, Marcos Paulo de Oliveira Cunha, Regimar Garcia dos Santos, Mayara Fávero Cotrim, Larissa Pereira Ribeiro Teodoro, Carlos Antonio da Silva Junior, Fabio Henrique Rojo Baio and Paulo Eduardo Teodoro
    Citation: Plant Methods 2022 18:13
  14. Rosette leaf trichomes of Arabidopsis thaliana have been broadly used to study cell development, cell differentiation and, more recently, cell wall biogenesis. However, trichome-specific biochemical or -omics ana...

    Authors: Jan W. Huebbers, Kim Büttgen, Franz Leissing, Melissa Mantz, Markus Pauly, Pitter F. Huesgen and Ralph Panstruga
    Citation: Plant Methods 2022 18:12
  15. Plant plasma membrane transporters play essential roles during the translocation of vectorized agrochemicals. Therefore, transporters associated with phloem loading of vectorized agrochemicals have drawn incre...

    Authors: Yongxin Xiao, Jinying Zhang, Yiting Li, Tom Hsiang, Xingping Zhang, Yongxing Zhu, Xiaoying Du, Junliang Yin and Junkai Li
    Citation: Plant Methods 2022 18:11
  16. Dendrobium nobile Lindl. is an important pharmacopeial plant with medicinal and ornamental value. This study sought to provide a technical means for the large-scale production of total alkaloid in D. nobile. Seed...

    Authors: Benhou Zhang, Zhitao Niu, Chao Li, Zhenyu Hou, Qingyun Xue, Wei Liu and Xiaoyu Ding
    Citation: Plant Methods 2022 18:10
  17. Rice is a major staple food crop for more than half the world’s population. As the global population is expected to reach 9.7 billion by 2050, increasing the production of high-quality rice is needed to meet t...

    Authors: Chaoxin Wang, Doina Caragea, Nisarga Kodadinne Narayana, Nathan T. Hein, Raju Bheemanahalli, Impa M. Somayanda and S. V. Krishna Jagadish
    Citation: Plant Methods 2022 18:9
  18. Leaf hairiness (pubescence) is an important plant phenotype which regulates leaf transpiration, affects sunlight penetration, and provides increased resistance or susceptibility against certain insects. Cotton...

    Authors: Vivien Rolland, Moshiur R. Farazi, Warren C. Conaty, Deon Cameron, Shiming Liu, Lars Petersson and Warwick N. Stiller
    Citation: Plant Methods 2022 18:8
  19. Ralstonia solanacearum, one of the most devastating bacterial plant pathogens, is the causal agent of bacterial wilt. Recently, several studies on resistance to bacterial wilt have been conducted using the Arabid...

    Authors: Cuihong Xu, Lingkun Zhong, Zeming Huang, Chenying Li, Jiazhang Lian, Xuefang Zheng and Yan Liang
    Citation: Plant Methods 2022 18:7
  20. The incorporation of root traits into elite germplasm is typically a slow process. Thus, innovative approaches are required to accelerate research and pre-breeding programs targeting root traits to improve yie...

    Authors: Charlotte Rambla, Sarah Van Der Meer, Kai P. Voss-Fels, Manar Makhoul, Christian Obermeier, Rod Snowdon, Eric S. Ober, Michelle Watt, Samir Alahmad and Lee T. Hickey
    Citation: Plant Methods 2022 18:2
  21. Lupins are promising protein crops with an increasing amount of genomic and transcriptomic resources. The new resources facilitate the in silico identification of candidate genes controlling important agronomi...

    Authors: Davide Mancinotti, Maria Cecilia Rodriguez, Karen Michiko Frick, Bjørn Dueholm, Ditte Goldschmidt Jepsen, Niels Agerbirk and Fernando Geu-Flores
    Citation: Plant Methods 2021 17:131
  22. With increasing joint research cooperation on national and international levels, there is a high need for harmonized and reproducible cultivation conditions and experimental protocols in order to ensure the be...

    Authors: Paul Grünhofer, Yayu Guo, Ruili Li, Jinxing Lin and Lukas Schreiber
    Citation: Plant Methods 2021 17:129
  23. The leaf epidermis functions to prevent the loss of water and reduce gas exchange. As an interface between the plant and its external environment, it helps prevent damage, making it an attractive system for st...

    Authors: Jieping Li, Xinlei Feng and Jinjin Xie
    Citation: Plant Methods 2021 17:128
  24. 3D imaging, such as X-ray CT and MRI, has been widely deployed to study plant root structures. Many computational tools exist to extract coarse-grained features from 3D root images, such as total volume, root ...

    Authors: Dan Zeng, Mao Li, Ni Jiang, Yiwen Ju, Hannah Schreiber, Erin Chambers, David Letscher, Tao Ju and Christopher N. Topp
    Citation: Plant Methods 2021 17:127
  25. The bacterial leaf pathogen Pseudomonas syringae pv tomato (Pst) is the most popular model pathogen for plant pathology research. Previous methods to study the plant-Pst interactions rely on destructive quantific...

    Authors: Leonardo Furci, David Pascual-Pardo and Jurriaan Ton
    Citation: Plant Methods 2021 17:126
  26. The root system architecture (RSA) of alfalfa (Medicago sativa L.) affects biomass production by influencing water and nutrient uptake, including nitrogen fixation. Further, roots are important for storing carboh...

    Authors: Bruna Bucciarelli, Zhanyou Xu, Samadangla Ao, Yuanyuan Cao, Maria J. Monteros, Christopher N. Topp and Deborah A. Samac
    Citation: Plant Methods 2021 17:125
  27. Ribo-seq has revolutionized the study of genome-wide mRNA translation. High-quality Ribo-seq data display strong 3-nucleotide (nt) periodicity, which corresponds to translating ribosomes deciphering three nts ...

    Authors: Hsin-Yen Larry Wu and Polly Yingshan Hsu
    Citation: Plant Methods 2021 17:124
  28. Breakthrough imaging technologies may challenge the plant phenotyping bottleneck regarding marker-assisted breeding and genetic mapping. In this context, X-Ray CT (computed tomography) technology can accuratel...

    Authors: Monica Herrero-Huerta, Valerian Meline, Anjali S. Iyer-Pascuzzi, Augusto M. Souza, Mitchell R. Tuinstra and Yang Yang
    Citation: Plant Methods 2021 17:123
  29. Blackleg disease, caused by the fungal pathogen Leptosphaeria maculans, is a serious threat to canola (Brassica napus) production worldwide. Quantitative resistance to this disease is a highly desirable trait but...

    Authors: Wendelin Schnippenkoetter, Mohammad Hoque, Rebecca Maher, Angela Van de Wouw, Phillip Hands, Vivien Rolland, Luke Barrett and Susan Sprague
    Citation: Plant Methods 2021 17:122
  30. The rice gall midge (RGM, Orseolia oryzae, Wood-Mason), an important stem-feeding pest worldwide, has caused serious production losses over the past decades. Rice production practices indicate that the most relia...

    Authors: Ling Cheng, Fugang Huang, Zhe Jiang, Baiyi Lu, Xiaohui Zhong and Yongfu Qiu
    Citation: Plant Methods 2021 17:121
  31. Low temperature freezing stress has adverse effects on wheat seedling growth and final yield. The traditional method to evaluate the wheat injury caused by the freezing stress is by visual observations, which ...

    Authors: Xiuqing Fu, Yang Bai, Jing Zhou, Hongwen Zhang and Jieyu Xian
    Citation: Plant Methods 2021 17:120
  32. Due to the high cost of data collection for magnetization detection of media, the sample size is limited, it is not suitable to use deep learning method to predict its change trend. The prediction of physical ...

    Authors: Jing Nie, Nianyi Wang, Jingbin Li, Kang Wang and Hongkun Wang
    Citation: Plant Methods 2021 17:119
  33. Realizing imaging detection of water and nitrogen content in different regions of plant leaves in-site and real-time can provide an efficient new technology for determining crop drought resistance and nutrient...

    Authors: Ning Zhang, Peng-cheng Li, Hubin Liu, Tian-cheng Huang, Han Liu, Yu Kong, Zhi-cheng Dong, Yu-hui Yuan, Long-lian Zhao and Jun-hui Li
    Citation: Plant Methods 2021 17:117
  34. Macrophytes are key players in aquatic ecosystems diversity, but knowledge on variability of their functional traits, among and within species, is still limited. Remote sensing is a high-throughput, feasible o...

    Authors: Paolo Villa, Rossano Bolpagni, Monica Pinardi and Viktor R. Tóth
    Citation: Plant Methods 2021 17:115
  35. Multiplex CRISPR-Cas9-based genome editing is an efficient method for targeted disruption of gene function in plants. Use of CRISPR-Cas9 has increased rapidly in recent years and is becoming a routine method f...

    Authors: Robertas Ursache, Satoshi Fujita, Valérie Dénervaud Tendon and Niko Geldner
    Citation: Plant Methods 2021 17:111
  36. Grapevine, as an essential fruit crop with high economic values, has been the focus of molecular studies in diverse areas. Two challenges exist in the grapevine research field: (i) the lack of a rapid, user-fr...

    Authors: Yashu Song, Robert H. Hanner and Baozhong Meng
    Citation: Plant Methods 2021 17:110

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