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  1. The mutualistic interaction between entomophilous plants and pollinators is fundamental to the structure of most terrestrial ecosystems. The sensitive nature of this relationship has been disrupted by anthropo...

    Authors: Sydney B. Wizenberg, Laura R. Newburn, Mateus Pepinelli, Ida M. Conflitti, Rodney T. Richardson, Shelley E. R. Hoover, Robert W. Currie, Pierre Giovenazzo and Amro Zayed
    Citation: Plant Methods 2023 19:120
  2. Single-cell RNA sequencing (scRNA-seq) measurements of gene expression show great promise for studying the cellular heterogeneity of rice roots. How precisely annotating cell identity is a major unresolved pro...

    Authors: Hao Wang, Yu-Nan Lin, Shen Yan, Jing-Peng Hong, Jia-Rui Tan, Yan-Qing Chen, Yong-Sheng Cao and Wei Fang
    Citation: Plant Methods 2023 19:119
  3. Crop breeding should be accelerated to address global warming and climate change. Wheat (Triticum aestivum L.) is a major food crop. Speed breeding (SB) and speed vernalization (SV) techniques for spring and wint...

    Authors: Jin-Kyung Cha, Hyeonjin Park, Changhyun Choi, Youngho Kwon, So-Myeong Lee, Ki-Won Oh, Jong-Min Ko, Soon-Wook Kwon and Jong-Hee Lee
    Citation: Plant Methods 2023 19:118
  4. Although previous studies on the droplet deposition behaviour of rice leaves have modelled the leaves as flat surface structures, their curved surface structures actually have a significant effect on droplet d...

    Authors: He Zheng, Hao Sun, Yubin Cao, Xiaolan Lv, Chaoxi Wang, Yunfu Chen, Hongfeng Yu and Wei Qiu
    Citation: Plant Methods 2023 19:116
  5. Spike is the grain-bearing organ in cereal crops, which is a key proxy indicator determining the grain yield and quality. Machine learning methods for image analysis of spike-related phenotypic traits not only...

    Authors: Yimin Ling, Qinlong Zhao, Wenxin Liu, Kexu Wei, Runfei Bao, Weining Song and Xiaojun Nie
    Citation: Plant Methods 2023 19:115
  6. Rubus species holds promise as a valuable source of polyphenols and bioactive compounds, offering significant potential as functional food ingredients with both nutraceutical and pharmaceutical benefits. However,...

    Authors: Saurav Chandra Bhatt, Bindu Naik, Vijay Kumar, Arun Kumar Gupta, Sanjay Kumar, Manpreet Singh Preet, Nitya Sharma and Sarvesh Rustagi
    Citation: Plant Methods 2023 19:114
  7. Protein farnesylation involves the addition of a 15-carbon polyunsaturated farnesyl group to proteins whose C-terminus ends with a CaaX motif. This post-translational protein modification is catalyzed by a het...

    Authors: Jia-Rong Wu, Rida Zohra, Ngoc Kieu Thi Duong, Ching-Hui Yeh, Chung-An Lu and Shaw-Jye Wu
    Citation: Plant Methods 2023 19:113
  8. Long read sequencing allows the analysis of full-length transcripts in plants without the challenges of reliable transcriptome assembly. Long read sequencing of transcripts from plant genomes has often utilize...

    Authors: Othman Al-Dossary, Agnelo Furtado, Ardashir KharabianMasouleh, Bader Alsubaie, Ibrahim Al-Mssallem and Robert J. Henry
    Citation: Plant Methods 2023 19:112
  9. This study aims to establish cell suspension culture, its maintenance and induction of somatic embryogenesis, and in vitro plant regeneration in Cenchrus ciliaris L. Suspension cultures are relatively homogenous ...

    Authors: Shipra Goyal, Vijaya Chatterjee, Vishvas M. Kulkarni and Vishnu Bhat
    Citation: Plant Methods 2023 19:110
  10. Remote sensing of vegetation by spectroscopy is increasingly used to characterize trait distributions in plant communities. How leaves interact with electromagnetic radiation is determined by their structure a...

    Authors: Cheng Li, Ewa A. Czyż, Rayko Halitschke, Ian T. Baldwin, Michael E. Schaepman and Meredith C. Schuman
    Citation: Plant Methods 2023 19:108
  11. The field of plant hormonomics focuses on the qualitative and quantitative analysis of the hormone complement in plant samples, akin to other omics sciences. Plant hormones, alongside primary and secondary met...

    Authors: Ondřej Vrobel and Petr Tarkowski
    Citation: Plant Methods 2023 19:107
  12. Modern field pea breeding faces a significant challenge in selecting lines with strong stems that resist lodging. Traditional methods of assessing stem strength involve destructive mechanical tests on mature s...

    Authors: Qi Fang, Felipe A. Castro-Urrea, Felix Haederle, Rowan W. Sanderson, Dilusha Silva, Wallace A. Cowling and Brendan F. Kennedy
    Citation: Plant Methods 2023 19:105
  13. Since its discovery, somatic hybridization has been used to overcome the sexual barriers between cultivated and wild species. A combination of two somatic cells might provide a novel set of features, often of ...

    Authors: Katarzyna Mackowska, Katarzyna Stelmach-Wityk and Ewa Grzebelus
    Citation: Plant Methods 2023 19:104
  14. Detection and counting of wheat heads are of crucial importance in the field of plant science, as they can be used for crop field management, yield prediction, and phenotype analysis. With the widespread appli...

    Authors: Jianxiong Ye, Zhenghong Yu, Yangxu Wang, Dunlu Lu and Huabing Zhou
    Citation: Plant Methods 2023 19:103
  15. Common beans (Phaseolus vulgaris L.) provide important protein and calories globally. Anthracnose (Colletotrichum lindemuthianum (Sacc. & Magnus) Briosi & Cavara, 1889) is a major disease in common bean and cause...

    Authors: Marysia Zaleski-Cox, Phillip N. Miklas, Alvaro Soler-Garzón and Valerio Hoyos-Villegas
    Citation: Plant Methods 2023 19:102
  16. Sugarcane (Saccharum spp.) is the core crop for sugar and bioethanol production over the world. A major problem in sugarcane production is stalk lodging due to weak mechanical strength. Rind penetrometer resistan...

    Authors: Yinjuan Shen, Muhammad Adnan, Fumin Ma, Liyuan Kong, Maoyao Wang, Fuhong Jiang, Qian Hu, Wei Yao, Yongfang Zhou, Muqing Zhang and Jiangfeng Huang
    Citation: Plant Methods 2023 19:101
  17. Land plants exhibit a haplodiplontic life cycle, whereby multicellular bodies develop in both the haploid and diploid generations. The early-diverging land plants, known as bryophytes, have a haploid-dominant ...

    Authors: Emiko Yoro, Shizuka Koshimizu, Takashi Murata and Keiko Sakakibara
    Citation: Plant Methods 2023 19:100
  18. The diploid woodland strawberry (Fragaria vesca) is an excellent model plant for investigating economically significant traits and several genetic resources within the Rosaceae family. Agrobacterium rhizogenes-me...

    Authors: Huiqing Yan, Dandan Ma, Peipei Yi, Guilian Sun, Xingyan Chen, Yin Yi and Xiaolong Huang
    Citation: Plant Methods 2023 19:99
  19. The common tea tree disease known as “tea coal disease” (Neocapnodium theae Hara) can have a negative impact on tea yield and quality. The majority of conventional approaches for identifying tea coal disease rely...

    Authors: Yang Xu, Yilin Mao, He Li, Litao Sun, Shuangshuang Wang, Xiaojiang Li, Jiazhi Shen, Xinyue Yin, Kai Fan, Zhaotang Ding and Yu Wang
    Citation: Plant Methods 2023 19:98
  20. The determination of nutrient content in the petiole is one of the important methods for achieving cotton fertilization management. The establishment of a monitoring system for the nutrient content of cotton p...

    Authors: Zhiqiang Dong, Yang Liu, Minghua Li, Baoxia Ci, Xiaokang Feng, Shuai Wen, Xi Lu, Zheng He and Fuyu Ma
    Citation: Plant Methods 2023 19:97
  21. Genomic prediction has become a powerful modelling tool for assessing line performance in plant and livestock breeding programmes. Among the genomic prediction modelling approaches, linear based models have pr...

    Authors: Qingsen Yan, Mario Fruzangohar, Julian Taylor, Dong Gong, James Walter, Adam Norman, Javen Qinfeng Shi and Tristan Coram
    Citation: Plant Methods 2023 19:96
  22. Duckweeds, a family of floating aquatic plants, are ideal model plants for laboratory experiments because they are small, easy to cultivate, and reproduce quickly. Duckweed cultivation, for the purposes of sci...

    Authors: Madeline Scott, Orlando de Lange, Xavaar Quaranto, Ryan Cardiff and Eric Klavins
    Citation: Plant Methods 2023 19:95
  23. Virus-induced gene silencing (VIGS) is a reverse genetics technology that can efficiently and rapidly identify plant gene functions. Although a variety of VIGS vectors have been successfully used in plants, on...

    Authors: Xiaoyu Qi, Qiaoping Mo, Jing Li, Zhibo Zi, Mengyun Xu, Suju Yue, Hongbo Zhao, Haisheng Zhu and Guoping Wang
    Citation: Plant Methods 2023 19:94
  24. Sweetpotato is an important vegetable and food crop that is bred through sexual crosses and systematic selection. The use of in vitro germination of sweetpotato pollen to test its viability has important theor...

    Authors: Zongkuan Weng, Yitong Deng, Fen Tang, Lukuan Zhao, Lingxiao Zhao, Yuan Wang, Xibin Dai, Zhilin Zhou and Qinghe Cao
    Citation: Plant Methods 2023 19:93
  25. In plant breeding, one of the most cost-effective and efficient ways to increase genetic gain is to reduce the breeding cycle time. In general, modern breeding methods for self-pollinated crops should strive t...

    Authors: Milcah Kigoni, Megan Choi and Juan David Arbelaez
    Citation: Plant Methods 2023 19:92
  26. Biomass accumulation as a growth indicator can be significant in achieving high and stable soybean yields. More robust genotypes have a better potential for exploiting available resources such as water or sunl...

    Authors: Predrag Ranđelović, Vuk Đorđević, Jegor Miladinović, Slaven Prodanović, Marina Ćeran and Johann Vollmann
    Citation: Plant Methods 2023 19:89
  27. Glandular trichomes, often referred to as “phytochemical factories”, plays a crucial role in plant growth and metabolism. As the site for secretion and storage, the development of glandular trichomes is relate...

    Authors: Zheng Jiang, Peina Zhou, Yongfang Shao, Qianqian Zhang, Wei Yue, Cheng Qu and Qinan Wu
    Citation: Plant Methods 2023 19:88
  28. Efficient and site-specific weed management is a critical step in many agricultural tasks. Image captures from drones and modern machine learning based computer vision methods can be used to assess weed infest...

    Authors: Nikita Genze, Maximilian Wirth, Christian Schreiner, Raymond Ajekwe, Michael Grieb and Dominik G. Grimm
    Citation: Plant Methods 2023 19:87
  29. Rust is a damaging disease affecting vital crops, including pea, and identifying highly resistant genotypes remains a challenge. Accurate measurement of infection levels in large germplasm collections is cruci...

    Authors: Salvador Osuna-Caballero, Tiago Olivoto, Manuel A. Jiménez-Vaquero, Diego Rubiales and Nicolas Rispail
    Citation: Plant Methods 2023 19:86
  30. High-purity RNA serves as the basic requirement for downstream molecular analysis of plant species, especially the differential expression of genes to various biotic and abiotic stimuli. But, the extraction of...

    Authors: Shina Sasi, Saranya Krishnan, Preshobha Kodackattumannil, Aysha AL Shamisi, Maitha Aldarmaki, Geetha Lekshmi, Martin Kottackal and Khaled M. A. Amiri
    Citation: Plant Methods 2023 19:84
  31. Tar spot of corn is a significant and spreading disease in the continental U.S. and Canada caused by the obligate biotrophic fungus Phyllachora maydis. As of 2023, tar spot had been reported in 18 U.S. states and...

    Authors: José E. Solórzano, Shea E. Issendorf, Milton T. Drott, Jill C. Check, Emily M. Roggenkamp, C. D. Cruz, Nathan M. Kleczewski, Carlos C. Gongóra-Canul and Dean K. Malvick
    Citation: Plant Methods 2023 19:83
  32. Pumpkin seeds are major oil crops with high nutritional value and high oil content. The collection and identification of different pumpkin germplasm resources play a significant role in the realization of prec...

    Authors: Xiyao Li, Xuping Feng, Hui Fang, Ningyuan Yang, Guofeng Yang, Zeyu Yu, Jia Shen, Wei Geng and Yong He
    Citation: Plant Methods 2023 19:82
  33. Though multicolour labelling methods allow the routine detection of a wide range of fluorescent (immuno)probe types in molecular cytogenetics, combined applications for the simultaneous in situ detection of pr...

    Authors: Diána Makai, Edit Mihók, Dávid Polgári, András Cseh, Andrea Lenykó-Thegze, Adél Sepsi and László Sági
    Citation: Plant Methods 2023 19:80
  34. Large-scale data on the photosynthetic characteristics of whole crop canopy is crucial for improving yield. However, current data collection methods remain challenging, and the time constraints associated with...

    Authors: Yu Hyun Moon, Ui Jeong Woo, Ha Seon Sim, Tae Yeon Lee, Ha Rang Shin, Jung Su Jo and Sung Kyeom Kim
    Citation: Plant Methods 2023 19:79
  35. Plant virus vectors designed for virus-mediated protein overexpression (VOX), virus-induced gene silencing (VIGS), and genome editing (VIGE) provide rapid and cost-effective tools for functional genomics studi...

    Authors: Decai Tuo, Yuan Yao, Pu Yan, Xin Chen, Feihong Qu, Weiqian Xue, Jinping Liu, Hua Kong, Jianchun Guo, Hongguang Cui, Zhaoji Dai and Wentao Shen
    Citation: Plant Methods 2023 19:78
  36. The morphological structure phenotype of maize tassel plays an important role in plant growth, reproduction, and yield formation. It is an important step in the distinctness, uniformity, and stability (DUS) te...

    Authors: Wenqi Zhang, Sheng Wu, Weiliang Wen, Xianju Lu, Chuanyu Wang, Wenbo Gou, Yuankun Li, Xinyu Guo and Chunjiang Zhao
    Citation: Plant Methods 2023 19:76
  37. Verticillium wilt is the major disease of cotton, which would cause serious yield reduction and economic losses, and the identification of cotton verticillium wilt is of great significance to cotton research. ...

    Authors: Zhihao Lu, Shihao Huang, Xiaojun Zhang, Yuxuan shi, Wanneng Yang, Longfu Zhu and Chenglong Huang
    Citation: Plant Methods 2023 19:75
  38. A commonly used approach to study the interaction of two proteins of interest (POIs) in vivo is measuring Förster Resonance Energy Transfer (FRET). This requires the expression of the two POIs fused to two flu...

    Authors: Jan Eric Maika, Benedikt Krämer, Vivien I. Strotmann, Frank Wellmer, Stefanie Weidtkamp-Peters, Yvonne Stahl and Rüdiger Simon
    Citation: Plant Methods 2023 19:73
  39. Alternative splicing (AS) of mRNA plays an important roles in transcriptome diversity, involving regulation of plant growth and stress response. Understanding the variation of AS events underlying GWAS loci in...

    Authors: Siyuan Wang, Hongyu Wu, Yongyan Zhao, Luyao Wang, Xueying Guan and Ting Zhao
    Citation: Plant Methods 2023 19:72
  40. Investigations into the growth and self-organization of plant roots is subject to fundamental and applied research in various areas such as botany, agriculture, and soil science. The growth activity of the pla...

    Authors: Yalda Davoudpour, Steffen Kümmel, Niculina Musat, Hans Hermann Richnow and Matthias Schmidt
    Citation: Plant Methods 2023 19:71

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