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  1. With increasing joint research cooperation on national and international levels, there is a high need for harmonized and reproducible cultivation conditions and experimental protocols in order to ensure the be...

    Authors: Paul Grünhofer, Yayu Guo, Ruili Li, Jinxing Lin and Lukas Schreiber
    Citation: Plant Methods 2021 17:129
  2. The leaf epidermis functions to prevent the loss of water and reduce gas exchange. As an interface between the plant and its external environment, it helps prevent damage, making it an attractive system for st...

    Authors: Jieping Li, Xinlei Feng and Jinjin Xie
    Citation: Plant Methods 2021 17:128
  3. 3D imaging, such as X-ray CT and MRI, has been widely deployed to study plant root structures. Many computational tools exist to extract coarse-grained features from 3D root images, such as total volume, root ...

    Authors: Dan Zeng, Mao Li, Ni Jiang, Yiwen Ju, Hannah Schreiber, Erin Chambers, David Letscher, Tao Ju and Christopher N. Topp
    Citation: Plant Methods 2021 17:127
  4. The bacterial leaf pathogen Pseudomonas syringae pv tomato (Pst) is the most popular model pathogen for plant pathology research. Previous methods to study the plant-Pst interactions rely on destructive quantific...

    Authors: Leonardo Furci, David Pascual-Pardo and Jurriaan Ton
    Citation: Plant Methods 2021 17:126
  5. The root system architecture (RSA) of alfalfa (Medicago sativa L.) affects biomass production by influencing water and nutrient uptake, including nitrogen fixation. Further, roots are important for storing carboh...

    Authors: Bruna Bucciarelli, Zhanyou Xu, Samadangla Ao, Yuanyuan Cao, Maria J. Monteros, Christopher N. Topp and Deborah A. Samac
    Citation: Plant Methods 2021 17:125
  6. Ribo-seq has revolutionized the study of genome-wide mRNA translation. High-quality Ribo-seq data display strong 3-nucleotide (nt) periodicity, which corresponds to translating ribosomes deciphering three nts ...

    Authors: Hsin-Yen Larry Wu and Polly Yingshan Hsu
    Citation: Plant Methods 2021 17:124
  7. Breakthrough imaging technologies may challenge the plant phenotyping bottleneck regarding marker-assisted breeding and genetic mapping. In this context, X-Ray CT (computed tomography) technology can accuratel...

    Authors: Monica Herrero-Huerta, Valerian Meline, Anjali S. Iyer-Pascuzzi, Augusto M. Souza, Mitchell R. Tuinstra and Yang Yang
    Citation: Plant Methods 2021 17:123
  8. Blackleg disease, caused by the fungal pathogen Leptosphaeria maculans, is a serious threat to canola (Brassica napus) production worldwide. Quantitative resistance to this disease is a highly desirable trait but...

    Authors: Wendelin Schnippenkoetter, Mohammad Hoque, Rebecca Maher, Angela Van de Wouw, Phillip Hands, Vivien Rolland, Luke Barrett and Susan Sprague
    Citation: Plant Methods 2021 17:122
  9. The rice gall midge (RGM, Orseolia oryzae, Wood-Mason), an important stem-feeding pest worldwide, has caused serious production losses over the past decades. Rice production practices indicate that the most relia...

    Authors: Ling Cheng, Fugang Huang, Zhe Jiang, Baiyi Lu, Xiaohui Zhong and Yongfu Qiu
    Citation: Plant Methods 2021 17:121
  10. Low temperature freezing stress has adverse effects on wheat seedling growth and final yield. The traditional method to evaluate the wheat injury caused by the freezing stress is by visual observations, which ...

    Authors: Xiuqing Fu, Yang Bai, Jing Zhou, Hongwen Zhang and Jieyu Xian
    Citation: Plant Methods 2021 17:120
  11. Due to the high cost of data collection for magnetization detection of media, the sample size is limited, it is not suitable to use deep learning method to predict its change trend. The prediction of physical ...

    Authors: Jing Nie, Nianyi Wang, Jingbin Li, Kang Wang and Hongkun Wang
    Citation: Plant Methods 2021 17:119
  12. Realizing imaging detection of water and nitrogen content in different regions of plant leaves in-site and real-time can provide an efficient new technology for determining crop drought resistance and nutrient...

    Authors: Ning Zhang, Peng-cheng Li, Hubin Liu, Tian-cheng Huang, Han Liu, Yu Kong, Zhi-cheng Dong, Yu-hui Yuan, Long-lian Zhao and Jun-hui Li
    Citation: Plant Methods 2021 17:117
  13. Macrophytes are key players in aquatic ecosystems diversity, but knowledge on variability of their functional traits, among and within species, is still limited. Remote sensing is a high-throughput, feasible o...

    Authors: Paolo Villa, Rossano Bolpagni, Monica Pinardi and Viktor R. Tóth
    Citation: Plant Methods 2021 17:115
  14. Multiplex CRISPR-Cas9-based genome editing is an efficient method for targeted disruption of gene function in plants. Use of CRISPR-Cas9 has increased rapidly in recent years and is becoming a routine method f...

    Authors: Robertas Ursache, Satoshi Fujita, Valérie Dénervaud Tendon and Niko Geldner
    Citation: Plant Methods 2021 17:111
  15. Grapevine, as an essential fruit crop with high economic values, has been the focus of molecular studies in diverse areas. Two challenges exist in the grapevine research field: (i) the lack of a rapid, user-fr...

    Authors: Yashu Song, Robert H. Hanner and Baozhong Meng
    Citation: Plant Methods 2021 17:110
  16. Cellular components are controlled by genetic and physiological factors that define their shape and size. However, quantitively capturing the morphological characteristics and movement of cellular organelles f...

    Authors: Arpan Kumar Basak, Mohamadreza Mirzaei, Kazimierz Strzałka and Kenji Yamada
    Citation: Plant Methods 2021 17:109
  17. The need for rapid in-field measurement of key traits contributing to yield over many thousands of genotypes is a major roadblock in crop breeding. Recently, leaf hyperspectral reflectance data has been used t...

    Authors: Robert T. Furbank, Viridiana Silva-Perez, John R. Evans, Anthony G. Condon, Gonzalo M. Estavillo, Wennan He, Saul Newman, Richard Poiré, Ashley Hall and Zhen He
    Citation: Plant Methods 2021 17:108
  18. The characteristics of light source have an important influence on the measurement performance of canopy reflectance spectrometer. The size of the effective irradiation area and the uniformity of the light int...

    Authors: Hongfeng Yu, Yongqian Ding, Huanliang Xu, Xueni Wu and Xianglin Dou
    Citation: Plant Methods 2021 17:107
  19. The Agrobacterium-mediated transient transformation, which proved effective in diverse plant species, has been widely applied for high-throughput gene function studies due to its simplicity, rapidity, and high ef...

    Authors: Yongpeng Li, Tiantian Chen, Wei Wang, Hang Liu, Xin Yan, Kuanyu Wu-Zhang, Wei Qin, Lihui Xie, Yaojie Zhang, Bowen Peng, Xinghao Yao, Chen Wang, Sadaf-Ilyas Kayani, Xueqing Fu, Ling Li and Kexuan Tang
    Citation: Plant Methods 2021 17:106
  20. Zinc (Zn) is an important nutrient for human beings, which is also an essential micronutrient for crop growth. This study investigated the role of Zn in coordinating the mineral elements absorption in modern w...

    Authors: Xiaolong Guo, Xiangyu Ma, Jialiang Zhang, Jinghuan Zhu, Tian Lu, Qifei Wang, Xiaoming Wang, Wei Hua and Shengbao Xu
    Citation: Plant Methods 2021 17:105
  21. Litchi chinensis Sonn. is an economically important fruit tree in tropical and subtropical regions. However, litchi functional genomics is severely hindered due to its recalcitrance to regeneration and stable tra...

    Authors: Yaqi Qin, Dan Wang, Jiaxin Fu, Zhike Zhang, Yonghua Qin, Guibing Hu and Jietang Zhao
    Citation: Plant Methods 2021 17:103
  22. Since the beginning of the use of reporter proteins for expression analysis, a variety of approaches have been developed and proposed; both qualitative and quantitative. The lack of simple methods for direct o...

    Authors: Alexander A. Tyurin, Aleksandra V. Suhorukova, Igor V. Deineko, Olga S. Pavlenko, Viktoriia A. Fridman and Irina V. Goldenkova-Pavlova
    Citation: Plant Methods 2021 17:102
  23. Flexural three-point bending tests are useful for characterizing the mechanical properties of plant stems. These tests can be performed with minimal sample preparation, thus allowing tests to be performed rela...

    Authors: Nathanael Martin-Nelson, Brandon Sutherland, Michael Yancey, Chung Shan Liao, Christopher J. Stubbs and Douglas D. Cook
    Citation: Plant Methods 2021 17:101
  24. Transgenic plant suspension cells show economic potential for the production of valuable bioproducts. The sugar starvation-inducible rice αAmy3 promoter, together with its signal peptide, is widely applied to pro...

    Authors: Desyanti Saulina Sinaga, Shin-Lon Ho, Chung-An Lu, Su-May Yu and Li-Fen Huang
    Citation: Plant Methods 2021 17:99
  25. Apricot is cultivated worldwide because of its high nutritive content and strong adaptability. Its flesh is delicious and has a unique and pleasant aroma. Apricot kernel is also consumed as nuts. The genome of...

    Authors: Chen Chen, Huimin Liu, Ningning Gou, Mengzhen Huang, Wanyu Xu, Xuchun Zhu, Mingyu Yin, Haikun Bai, Lin Wang and Ta-na Wuyun
    Citation: Plant Methods 2021 17:98
  26. Detailed datasets containing root system and its architecture in soil are required to improve understanding of resource capture by roots. However, most of the root study methods have paid little attention to m...

    Authors: Takuya Koyama, Shun Murakami, Toshihiko Karasawa, Masato Ejiri and Katsuhiro Shiono
    Citation: Plant Methods 2021 17:97
  27. Fractional vegetation cover (FVC) is an important basic parameter for the quantitative monitoring of the alpine grassland ecosystem on the Qinghai-Tibetan Plateau. Based on unmanned aerial vehicle (UAV) acquis...

    Authors: Xingchen Lin, Jianjun Chen, Peiqing Lou, Shuhua Yi, Yu Qin, Haotian You and Xiaowen Han
    Citation: Plant Methods 2021 17:96
  28. Ploidy manipulation is effective in seedless loquat breeding, in which flesh color is a key agronomic and economic trait. Few techniques are currently available for detecting the genotypes of polyploids in pla...

    Authors: Haiyan Wang, Jiangbo Dang, Di Wu, Zhongyi Xie, Shuang Yan, Jingnan Luo, Qigao Guo and Guolu Liang
    Citation: Plant Methods 2021 17:93
  29. Translation is a tightly regulated process, controlling the rate of protein synthesis in cells. Ribosome sequencing (Ribo-Seq) is a recently developed tool for studying actively translated mRNA and can thus di...

    Authors: Amir Mahboubi, Nicolas Delhomme, Sara Häggström and Johannes Hanson
    Citation: Plant Methods 2021 17:92
  30. Maize cobs are an important component of crop yield that exhibit a high diversity in size, shape and color in native landraces and modern varieties. Various phenotyping approaches were developed to measure mai...

    Authors: Lydia Kienbaum, Miguel Correa Abondano, Raul Blas and Karl Schmid
    Citation: Plant Methods 2021 17:91
  31. Estimation of nitrate nitrogen (NO3–N) content in petioles is one of the key approaches for monitoring nitrogen (N) nutrition in crops. Rapid, non-destructive, and accurate evaluation of NO3–N contents in cotto...

    Authors: Zhiqiang Dong, Yang Liu, Baoxia Ci, Ming Wen, Minghua Li, Xi Lu, Xiaokang Feng, Shuai Wen and Fuyu Ma
    Citation: Plant Methods 2021 17:90
  32. Since the introduction of studies on maize silage digestibility at the end of the nineteenth century, protocols to estimate dry matter digestibility have not stopped evolving. Since the early 1980s, the protoc...

    Authors: P.-L. Lopez-Marnet, S. Guillaume, M.-P. Jacquemot, M. Reymond and V. Méchin
    Citation: Plant Methods 2021 17:89
  33. Rice is one of the most important grain crops worldwide. The accurate and dynamic monitoring of Leaf Area Index (LAI) provides important information to evaluate rice growth and production.

    Authors: Yan Gong, Kaili Yang, Zhiheng Lin, Shenghui Fang, Xianting Wu, Renshan Zhu and Yi Peng
    Citation: Plant Methods 2021 17:88
  34. Hyperaccumulation of trace elements is a rare trait among plants which is being investigated to advance our understanding of the regulation of metal accumulation and applications in phytotechnologies. Noccaea cae...

    Authors: Lucas van der Zee, Amelia Corzo Remigio, Lachlan W. Casey, Imam Purwadi, Jitpanu Yamjabok, Antony van der Ent, Gert Kootstra and Mark G. M. Aarts
    Citation: Plant Methods 2021 17:86
  35. Heterosis has been widely used in maize breeding. However, we know little about the heterotic quantitative trait loci and their roles in genomic prediction. In this study, we sought to identify heterotic quant...

    Authors: Tifu Zhang, Lu Jiang, Long Ruan, Yiliang Qian, Shuaiqiang Liang, Feng Lin, Haiyan Lu, Huixue Dai and Han Zhao
    Citation: Plant Methods 2021 17:85
  36. Plant protoplasts constitute unique single-cell systems that can be subjected to genomic, proteomic, and metabolomic analysis. An effective and sustainable method for preparing protoplasts from tea plants has ...

    Authors: Xue-feng Xu, Hai-yan Zhu, Yin-feng Ren, Can Feng, Zhi-hao Ye, Hui-mei Cai, Xiao-chun Wan and Chuan-yi Peng
    Citation: Plant Methods 2021 17:84
  37. DNA–protein interactions are essential for several molecular and cellular mechanisms, such as transcription, transcriptional regulation, DNA modifications, among others. For many decades scientists tried to un...

    Authors: Ricardo André Campos Ferraz, Ana Lúcia Gonçalves Lopes, Jessy Ariana Faria da Silva, Diana Filipa Viana Moreira, Maria João Nogueira Ferreira and Sílvia Vieira de Almeida Coimbra
    Citation: Plant Methods 2021 17:82
  38. Drought is a major consequence of global heating that has negative impacts on agriculture. Potato is a drought-sensitive crop; tuber growth and dry matter content may both be impacted. Moreover, water deficit ...

    Authors: M. Musse, G. Hajjar, N. Ali, B. Billiot, G. Joly, J. Pépin, S. Quellec, S. Challois, F. Mariette, M. Cambert, C. Fontaine, C. Ngo-Dinh, F. Jamois, A. Barbary, P. Leconte, C. Deleu…
    Citation: Plant Methods 2021 17:81

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