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  1. Image processing techniques have been widely used in the analysis of leaf characteristics. Earlier techniques for processing digital RGB color images of plant leaves had several drawbacks, such as inadequate d...

    Authors: Zhengmeng Chen, Fuzheng Wang, Pei Zhang, Chendan Ke, Yan Zhu, Weixing Cao and Haidong Jiang

    Citation: Plant Methods 2020 16:23

    Content type: Research

    Published on:

  2. Aneuploidy, a condition caused by an imbalance between the relative dosages of chromosomes, generally produces a novel phenotype specific to the molecular karyotype. Few techniques are currently available for ...

    Authors: Guo Wen, Jiangbo Dang, Zhongyi Xie, Jinying Wang, Pengfei Jiang, Qigao Guo and Guolu Liang

    Citation: Plant Methods 2020 16:22

    Content type: Methodology

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  3. Myxospermy is a process by which the external surfaces of seeds of many plant species produce mucilage—a polysaccharide-rich gel with numerous fundamental research and industrial applications. Due to its funct...

    Authors: James M. Cowley, Lina Herliana, Kylie A. Neumann, Silvano Ciani, Virna Cerne and Rachel A. Burton

    Citation: Plant Methods 2020 16:20

    Content type: Research

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  4. Tea, which is produced from new shoots of existing tea plants (Camellia sinensis), is one of the most popular, non-alcoholic, healthy beverages worldwide. Colletotrichum camelliae is one of the dominant fungal pa...

    Authors: Shengnan He, Huchen Chen, Yi Wei, Tai An and Shouan Liu

    Citation: Plant Methods 2020 16:17

    Content type: Methodology

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  5. Forward genetic screens are a powerful approach for identifying the genes contributing to a trait of interest. However, mutants arising in genes already known can obscure the identification of new genes contri...

    Authors: Jiangling Cao, Nial R. Gursanscky, Stephen J. Fletcher, Anne Sawyer, Mehershad Wadia, Lachlan McKeough, Marek Coleman, Uwe Dressel, Christelle Taochy, Neena Mitter, Hervé Vaucheret and Bernard J. Carroll

    Citation: Plant Methods 2020 16:16

    Content type: Methodology

    Published on:

  6. Improving abiotic stress tolerance in wheat requires large scale screening of yield components such as seed weight, seed number and single seed weight, all of which is very laborious, and a detailed analysis o...

    Authors: Jessica Schmidt, Joelle Claussen, Norbert Wörlein, Anja Eggert, Delphine Fleury, Trevor Garnett and Stefan Gerth

    Citation: Plant Methods 2020 16:15

    Content type: Research

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  7. Plants, like all living organisms, metamorphose their bodies during their lifetime. All the developmental and growth events in a plant’s life are connected to specific points in time, be it seed germination, s...

    Authors: Jan F. Humplík, Jakub Dostál, Lydia Ugena, Lukáš Spíchal, Nuria De Diego, Ondřej Vencálek and Tomáš Fürst

    Citation: Plant Methods 2020 16:14

    Content type: Methodology

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  8. Plant root research can provide a way to attain stress-tolerant crops that produce greater yield in a diverse array of conditions. Phenotyping roots in soil is often challenging due to the roots being difficul...

    Authors: Abraham George Smith, Jens Petersen, Raghavendra Selvan and Camilla Ruø Rasmussen

    Citation: Plant Methods 2020 16:13

    Content type: Methodology

    Published on:

  9. Variable-rate fertilization is crucial in the implementation of precision agriculture and for ensuring reasonable and efficient fertilizer application and nutrient management that is tailored to local conditio...

    Authors: Yinyan Shi, Yang Zhu, Xiaochan Wang, Xin Sun, Yangfen Ding, Wexing Cao and Zhichao Hu

    Citation: Plant Methods 2020 16:11

    Content type: Review

    Published on:

  10. Covalent modifications of histones and histone variants have great influence on chromatin structure, which is involved in the transcriptional regulation of gene expression. Chromatin immunoprecipitation (ChIP)...

    Authors: Xiaorong Huang, Qinwei Pan, Ying Lin, Tingting Gu and Yi Li

    Citation: Plant Methods 2020 16:10

    Content type: Methodology

    Published on:

  11. Cucurbits produce fruits or vegetables that have great dietary importance and economic significance worldwide. The published genomes of at least 11 cucurbit species are boosting gene mining and novel breeding ...

    Authors: Mei Liu, Zhiling Liang, Miguel A. Aranda, Ni Hong, Liming Liu, Baoshan Kang and Qinsheng Gu

    Citation: Plant Methods 2020 16:9

    Content type: Research

    Published on:

  12. The visualization of internal 3D-structure of tissues at micron resolutions without staining by contrast reagents is desirable in plant researches, and it can be achieved by an X-ray computed tomography (CT) w...

    Authors: Naoki Kunishima, Yoshihiro Takeda, Raita Hirose, Dominika Kalasová, Jakub Šalplachta and Kazuhiko Omote

    Citation: Plant Methods 2020 16:7

    Content type: Methodology

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  13. Root system architecture (RSA) traits are of interest for breeding selection; however, measurement of these traits is difficult, resource intensive, and results in large variability. The advent of computer vis...

    Authors: Kevin G. Falk, Talukder Z. Jubery, Seyed V. Mirnezami, Kyle A. Parmley, Soumik Sarkar, Arti Singh, Baskar Ganapathysubramanian and Asheesh K. Singh

    Citation: Plant Methods 2020 16:5

    Content type: Methodology

    Published on:

  14. The unfolded protein response (UPR) is a highly conserved process in eukaryotic organisms that plays a crucial role in adaptation and development. While the most ubiquitous components of this pathway have been...

    Authors: André Alcântara, Denise Seitner, Fernando Navarrete and Armin Djamei

    Citation: Plant Methods 2020 16:4

    Content type: Methodology

    Published on:

  15. Plant receptors with lysin motifs (LsyM) recognize microbial signals such as fungal chitin and lipo-chitooligosaccharidic Nod factors of nitrogen-fixing rhizobia. It is generally assumed that ligand-induced di...

    Authors: Chun-Lian Li, De-Xing Xue, Yi-Han Wang, Zhi-Ping Xie and Christian Staehelin

    Citation: Plant Methods 2020 16:3

    Content type: Methodology

    Published on:

  16. Micro-computed tomography (μCT) bring a new opportunity to accurately quantify micro phenotypic traits of maize stem, also provide comparable benchmark to evaluate its dynamic development at the different grow...

    Authors: Ying Zhang, Liming Ma, Jinglu Wang, Xiaodong Wang, Xinyu Guo and Jianjun Du

    Citation: Plant Methods 2020 16:2

    Content type: Research

    Published on:

  17. Herbaria are valuable sources of extensive curated plant material that are now accessible to genetic studies because of advances in high-throughput, next-generation sequencing methods. As an applied assessment...

    Authors: Paul G. Nevill, Xiao Zhong, Julian Tonti-Filippini, Margaret Byrne, Michael Hislop, Kevin Thiele, Stephen van Leeuwen, Laura M. Boykin and Ian Small

    Citation: Plant Methods 2020 16:1

    Content type: Methodology

    Published on:

  18. In the original publication [1], the copyright line was incorrectly published as “© The Author(s) 2019”. The corrected copyright line should read as “© Her Majesty the Queen in Right of Canada as represented b...

    Authors: Xiucheng Cui, Margaret Balcerzak, Johann Schernthaner, Vivijan Babic, Raju Datla, Elizabeth K. Brauer, Natalie Labbé, Rajagopal Subramaniam and Thérèse Ouellet

    Citation: Plant Methods 2019 15:163

    Content type: Correction

    Published on:

    The original article was published in Plant Methods 2019 15:119

  19. Recent advances in image-based plant phenotyping have improved our capability to study vegetative stage growth dynamics. However, more complex agronomic traits such as inflorescence architecture (IA), which pr...

    Authors: Jaspreet Sandhu, Feiyu Zhu, Puneet Paul, Tian Gao, Balpreet K. Dhatt, Yufeng Ge, Paul Staswick, Hongfeng Yu and Harkamal Walia

    Citation: Plant Methods 2019 15:162

    Content type: Methodology

    Published on:

  20. High grain breakage rate is the main limiting factor encountered in the mechanical harvest of maize grain. X-ray micro-computed tomography (μCT) scanning technology could be used to obtain the three-dimensiona...

    Authors: Junfeng Hou, Ying Zhang, Xiuliang Jin, Pengfei Dong, Yanan Guo, Keru Wang, Yinghu Fan and Shaokun Li

    Citation: Plant Methods 2019 15:161

    Content type: Research

    Published on:

  21. Almond is an emerging crop due to the health benefits of almond consumption including nutritional, anti-inflammatory, and hypocholesterolaemia properties. Traditional almond producers were concentrated in Cali...

    Authors: Francisca López-Granados, Jorge Torres-Sánchez, Francisco M. Jiménez-Brenes, Octavio Arquero, María Lovera and Ana I. de Castro

    Citation: Plant Methods 2019 15:160

    Content type: Research

    Published on:

  22. Seagrass meadows are recognized as critical and among the most vulnerable habitats on the planet. The alarming rates of decline in seagrass meadows have attracted the attention globally. There is an urgent nee...

    Authors: Shidong Yue, Yu Zhang, Yi Zhou, Shaochun Xu, Shuai Xu, Xiaomei Zhang and Ruiting Gu

    Citation: Plant Methods 2019 15:158

    Content type: Research

    Published on:

  23. The raised demand of cannabis as a medicinal plant in recent years led to an increased interest in understanding the biosynthetic routes of cannabis metabolites. Since there is no established protocol to gener...

    Authors: Julia Schachtsiek, Tajammul Hussain, Khadija Azzouhri, Oliver Kayser and Felix Stehle

    Citation: Plant Methods 2019 15:157

    Content type: Research

    Published on:

  24. Over the last years, several plant science labs have started to employ fluctuating growth light conditions to simulate natural light regimes more closely. Many plant mutants reveal quantifiable effects under f...

    Authors: Dominik Schneider, Laura S. Lopez, Meng Li, Joseph D. Crawford, Helmut Kirchhoff and Hans-Henning Kunz

    Citation: Plant Methods 2019 15:156

    Content type: Methodology

    Published on:

  25. Plant feeding, free-living nematodes cause extensive damage to plant roots by direct feeding and, in the case of some trichodorid and longidorid species, through the transmission of viruses. Developing more en...

    Authors: Felicity E. O’Callaghan, Roy Neilson, Stuart A. MacFarlane and Lionel X. Dupuy

    Citation: Plant Methods 2019 15:155

    Content type: Methodology

    Published on:

  26. Guayule (Parthenium argentatum A. Gray), a plant native to semi-arid regions of northern Mexico and southern Texas in the United States, is an alternative source for natural rubber (NR). Rapid screening tools are...

    Authors: Zinan Luo, Kelly R. Thorp and Hussein Abdel-Haleem

    Citation: Plant Methods 2019 15:154

    Content type: Methodology

    Published on:

  27. Acoustic emission (AE) sensing is in use since the late 1960s in drought-induced embolism research as a non-invasive and continuous method. It is very well suited to assess a plant’s vulnerability to dehydrati...

    Authors: Niels J. F. De Baerdemaeker, Michiel Stock, Jan Van den Bulcke, Bernard De Baets, Luc Van Hoorebeke and Kathy Steppe

    Citation: Plant Methods 2019 15:153

    Content type: Research

    Published on:

  28. In the original publication of the article [1], a few errors were identified.

    Authors: Sadiye Hayta, Mark A. Smedley, Selcen U. Demir, Robert Blundell, Alison Hinchliffe, Nicola Atkinson and Wendy A. Harwood

    Citation: Plant Methods 2019 15:152

    Content type: Correction

    Published on:

    The original article was published in Plant Methods 2019 15:121

  29. Plant development is controlled by the action of many, often connected gene regulatory networks. Differential gene expression controlled by internal and external cues is a major driver of growth and time speci...

    Authors: Kimmo Kivivirta, Denise Herbert, Matthias Lange, Knut Beuerlein, Janine Altmüller and Annette Becker

    Citation: Plant Methods 2019 15:151

    Content type: Methodology

    Published on:

  30. Deep rooting is one of the most promising plant traits for improving crop yield under water-limited conditions. Most root phenotyping methods are designed for laboratory-grown plants, typically measuring very ...

    Authors: Si Chen, Simon Fiil Svane and Kristian Thorup-Kristensen

    Citation: Plant Methods 2019 15:148

    Content type: Research

    Published on:

  31. Non-invasive procedures for the diagnosis of viability of plant or fungal tissues would be valuable for scientific, industrial and biomonitoring purposes. Previous studies showed that infrared thermography (IR...

    Authors: Beatriz Fernández-Marín, Othmar Buchner, Gerald Kastberger, Federica Piombino, José Ignacio García-Plazaola and Ilse Kranner

    Citation: Plant Methods 2019 15:147

    Content type: Research

    Published on:

  32. Potato, the third most important crop worldwide, plays a critical role in human food security. Brown rot, one of the most destructive potato diseases caused by Ralstonia solanacearum, results in huge economic los...

    Authors: Huijuan Wang, Jinxue Hu, Yao Lu, Mancang Zhang, Ning Qin, Ruize Zhang, Yizhe He, Dongdong Wang, Yue Chen, Cuizhu Zhao, Núria S. Coll, Marc Valls, Qin Chen and Haibin Lu

    Citation: Plant Methods 2019 15:145

    Content type: Research

    Published on:

  33. Insect herbivory poses a major threat to maize. Benzoxazinoids are important anti-insect secondary metabolites in maize, whose biosynthetic pathway has been extensively studied. However, yet little is known ab...

    Authors: Lei Gao, Guojing Shen, Lingdan Zhang, Jinfeng Qi, Cuiping Zhang, Canrong Ma, Jing Li, Lei Wang, Saif Ul Malook and Jianqiang Wu

    Citation: Plant Methods 2019 15:144

    Content type: Research

    Published on:

  34. Large insert paired-end sequencing technologies are important tools for assembling genomes, delineating associated breakpoints and detecting structural rearrangements. To facilitate the comprehensive detection...

    Authors: Zhaozhao Dai, Tong Li, Jiadong Li, Zhifei Han, Yonglong Pan, Sha Tang, Xianmin Diao and Meizhong Luo

    Citation: Plant Methods 2019 15:142

    Content type: Methodology

    Published on:

  35. Plant population density is an important factor for agricultural production systems due to its substantial influence on crop yield and quality. Traditionally, plant population density is estimated by using eit...

    Authors: Yu Jiang, Changying Li, Andrew H. Paterson and Jon S. Robertson

    Citation: Plant Methods 2019 15:141

    Content type: Research

    Published on:

  36. In the early stages of plant breeding programs high-quality phenotypes are still a constraint to improve genetic gain. New field-based high-throughput phenotyping (HTP) platforms have the capacity to rapidly a...

    Authors: Fabiana Freitas Moreira, Anthony Ahau Hearst, Keith Aric Cherkauer and Katy Martin Rainey

    Citation: Plant Methods 2019 15:139

    Content type: Research

    Published on:

  37. The demand for effective use of water resources has increased because of ongoing global climate transformations in the agriculture science sector. Cost-effective and timely distributions of the appropriate amo...

    Authors: Adnan Zahid, Hasan T. Abbas, Aifeng Ren, Ahmed Zoha, Hadi Heidari, Syed A. Shah, Muhammad A. Imran, Akram Alomainy and Qammer H. Abbasi

    Citation: Plant Methods 2019 15:138

    Content type: Research

    Published on:

  38. Predicting impact of plant tissue culture media components on explant proliferation is important especially in commercial scale for optimizing efficient culture media. Previous studies have focused on predicti...

    Authors: Saeid Jamshidi, Abbas Yadollahi, Mohammad Mehdi Arab, Mohammad Soltani, Maliheh Eftekhari, Hamed Sabzalipoor, Abdollatif Sheikhi and Jalal Shiri

    Citation: Plant Methods 2019 15:136

    Content type: Research

    Published on:

  39. Post-transcriptional gene regulation is one of the critical layers of overall gene expression programs and microRNAs (miRNAs) play an indispensable role in this process by guiding cleavage on the messenger RNA...

    Authors: Yong-Fang Li, Miao Zhao, Menglei Wang, Junqiang Guo, Li Wang, Jie Ji, Zongbo Qiu, Yun Zheng and Ramanjulu Sunkar

    Citation: Plant Methods 2019 15:134

    Content type: Methodology

    Published on:

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