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  1. Bamboo is a perennial and renewable biomass forest resource and its leaf flavonoid is an antioxidant for biological and pharmacological research. The established genetic transformation and gene editing systems...

    Authors: Huayu Sun, Sining Wang, Chenglei Zhu, Kebin Yang, Yan Liu and Zhimin Gao
    Citation: Plant Methods 2023 19:20
  2. Non-invasive reporter systems are powerful tools to query physiological and transcriptional responses in organisms. For example, fluorescent and bioluminescent reporters have revolutionized cellular and organi...

    Authors: Maria L. Sorkin, Kathleen K Markham, Stevan Zorich, Ananda Menon, Kristen N. Edgeworth, Angela Ricono, Douglas Bryant, Rebecca Bart, Dmitri A. Nusinow and Kathleen Greenham
    Citation: Plant Methods 2023 19:19
  3. Lignocellulosic biomass is a complex network of polysaccharides and lignin that requires a pretreatment step to overcome recalcitrance and optimize valorisation into biobased products. Pretreatment of biomass ...

    Authors: Edwige Audibert, Berangère Lebas, Corentin Spriet, Anouck Habrant, Brigitte Chabbert and Gabriel Paës
    Citation: Plant Methods 2023 19:16
  4. Agrobacterium tumefaciens-mediated leaf disc genetic transformation is an important way to achieve transgenics or gene editing. Ensuring stable and efficient genetic transformation is still an important problem i...

    Authors: Yufei Xia, Yuan Cao, Yongyu Ren, Aoyu Ling, Kang Du, Yun Li, Jun Yang and Xiangyang Kang
    Citation: Plant Methods 2023 19:15
  5. Herbicide resistance is one of the threats to modern agriculture and its early detection is one of the most effective components for sustainable resistance management strategies. Many techniques have been used...

    Authors: Silvia Panozzo, Silvia Farinati, Maurizio Sattin and Laura Scarabel
    Citation: Plant Methods 2023 19:14
  6. Despite the increased efficiency of sequencing technologies and the development of reduced-representation sequencing (RRS) approaches allowing high-throughput sequencing (HTS) of multiplexed samples, the per-s...

    Authors: Maxime de Ronne, Gaétan Légaré, François Belzile, Brian Boyle and Davoud Torkamaneh
    Citation: Plant Methods 2023 19:13
  7. Microscopic analysis of plant anatomy is a common procedure in biology to study structure and function that requires high-quality sections for accurate measurements. Hand sectioning of specimens is typically l...

    Authors: David J. Thomas, Jordan Rainbow and Laura E. Bartley
    Citation: Plant Methods 2023 19:12

    The Correction to this article has been published in Plant Methods 2024 20:58

  8. Crop breeding based on root system architecture (RSA) optimization is an essential factor for improving crop production in developing countries. Identification, evaluation, and selection of root traits of soil...

    Authors: Qian Wu, Jie Wu, Pengcheng Hu, Weixin Zhang, Yuntao Ma, Kun Yu, Yan Guo, Jing Cao, Huayong Li, Baiming Li, Yuyang Yao, Hongxin Cao and Wenyu Zhang
    Citation: Plant Methods 2023 19:11
  9. The number of soybean pods is one of the most important indicators of soybean yield, pod counting is crucial for yield estimation, cultivation management, and variety breeding. Counting pods manually is slow a...

    Authors: Shuai Xiang, Siyu Wang, Mei Xu, Wenyan Wang and Weiguo Liu
    Citation: Plant Methods 2023 19:8

    The Correction to this article has been published in Plant Methods 2023 19:45

  10. Karst vegetation is of great significance for ecological restoration in karst areas. Vegetation Indices (VIs) are mainly related to plant yield which is helpful to understand the status of ecological restorati...

    Authors: Wen Pan, Xiaoyu Wang, Yan Sun, Jia Wang, Yanjie Li and Sheng Li
    Citation: Plant Methods 2023 19:7
  11. As a result of the technological progress, the use of sensors for crop survey has substantially increased, generating valuable information for modelling agricultural data. Plant spectroscopy jointly with stati...

    Authors: Rosa Angela Pacheco-Gil, Ciro Velasco-Cruz, Paulino Pérez-Rodríguez, Juan Burgueño, Sergio Pérez-Elizalde, Francelino Rodrigues, Ivan Ortiz-Monasterio, David Hebert del Valle-Paniagua and Fernando Toledo
    Citation: Plant Methods 2023 19:6
  12. Cotton has tremendous economic value worldwide; however, its allopolyploid nature and time-consuming transformation methods have hampered the development of cotton functional genomics. The protoplast system ha...

    Authors: Ke Zhang, Shanhe Liu, Yunze Fu, Zixuan Wang, Xiubo Yang, Wenjing Li, Caihua Zhang, Dongmei Zhang and Jun Li
    Citation: Plant Methods 2023 19:5
  13. Cassava is the most cultivated and consumed root crop in the world. One of the major constraints to the cassava value chain is the short shelf life of cassava storage roots which is primarily due to the so-cal...

    Authors: Ima M. Zainuddin, Brieuc Lecart, Enny Sudarmonowati and Hervé Vanderschuren
    Citation: Plant Methods 2023 19:4
  14. This study presents a methodology for a high-throughput digitization and quantification process of plant cell walls characterization, including the automated development of two-dimensional finite element model...

    Authors: Andrew Sayad, Yusuf Oduntan, Norbert Bokros, Seth DeBolt, Alice Benzecry, Daniel J. Robertson and Christopher J. Stubbs
    Citation: Plant Methods 2023 19:3
  15. NADPH oxidase (Nox) plays a crucial role in reactive oxygen spices (ROS) production and mediates different diseases’ development. Under aerobic conditions, the NADPH-containing protein component of the (NCP)-F...

    Authors: Sona M. Feschyan, Ruzan M. Simonyan, Gegham M. Simonyan, Maxim A. Simonyan and Ashkhen L. Manukyan
    Citation: Plant Methods 2023 19:1
  16. In June 2020, severe symptoms of leaf mosaic and fruit malformation were observed on greenhouse-grown cucumber plants in Xizhou Township of Changhua County, Taiwan. An unknown virus, designated CX-2, was isola...

    Authors: Zi-Xuan Dong, Chian-Chi Lin, Yuh-Kun Chen, Chia-Cheng Chou and Tsung-Chi Chen
    Citation: Plant Methods 2022 18:143
  17. Camellia oleifera (C. oleifera) is a woody edible oil crop of great economic importance. Because of the lack of modern biotechnology research, C. oleifera faces huge challenges in both breeding and basic researc...

    Authors: Sufang Li, Rui Zhao, Tianwen Ye, Rui Guan, Linjie Xu, Xiaoling Ma, Jiaxi Zhang, Shixin Xiao and Deyi Yuan
    Citation: Plant Methods 2022 18:141
  18. “Herbarium X-ray Fluorescence (XRF) Ionomics” is a new quantitative approach for extracting the elemental concentrations from herbarium specimens using handheld XRF devices. These instruments are principally d...

    Authors: Imam Purwadi, Lachlan W. Casey, Chris G. Ryan, Peter D. Erskine and Antony van der Ent
    Citation: Plant Methods 2022 18:139
  19. Virtual plants can simulate the plant growth and development process through computer modeling, which assists in revealing plant growth and development patterns. Virtual plant visualization technology is a cor...

    Authors: Lingfeng Duan, Zhihao Wang, Hongfei Chen, Jinyang Fu, Hanzhi Wei, Zedong Geng and Wanneng Yang
    Citation: Plant Methods 2022 18:138
  20. Long-read sequencing platforms offered by Oxford Nanopore Technologies (ONT) allow native DNA containing epigenetic modifications to be directly sequenced, but can be limited by lower per-base accuracies. A ke...

    Authors: Scott Ferguson, Todd McLay, Rose L. Andrew, Jeremy J. Bruhl, Benjamin Schwessinger, Justin Borevitz and Ashley Jones
    Citation: Plant Methods 2022 18:137
  21. Frequency is essential in signal transmission, especially in convolutional neural networks. It is vital to maintain the signal frequency in the neural network to maintain the performance of a convolutional neu...

    Authors: Jiayu Zhang, Chao Qi, Peter Mecha, Yi Zuo, Zongyou Ben, Haolu Liu and Kunjie Chen
    Citation: Plant Methods 2022 18:136
  22. Cyst nematodes are one of the major groups of plant-parasitic nematode, responsible for considerable crop losses worldwide. Improving genetic resources, and therefore resistant cultivars, is an ongoing focus o...

    Authors: Olaf Prosper Kranse, Itsuhiro Ko, Roberta Healey, Unnati Sonawala, Siyuan Wei, Beatrice Senatori, Francesco De Batté, Ji Zhou and Sebastian Eves-van den Akker
    Citation: Plant Methods 2022 18:134
  23. Root system architecture (RSA) is an essential characteristic for efficient water and nutrient absorption in terrestrial plants; its plasticity enables plants to respond to different soil environments. Better ...

    Authors: Shota Teramoto and Yusaku Uga
    Citation: Plant Methods 2022 18:133
  24. Copy number determination is one of the first steps in the characterization of transgenic plant lines. The classical approach to this, Southern blotting, is time-consuming, expensive and requires massive amoun...

    Authors: Jonas De Saeger, Jihae Park, Kai Thoris, Charlotte De Bruyn, Hoo Sun Chung, Dirk Inzé and Stephen Depuydt
    Citation: Plant Methods 2022 18:132
  25. Seedling growth is an early phase of plant development highly susceptible to environmental factors such as soil nitrogen (N) availability or presence of seed-borne pathogens. Whereas N plays a central role in ...

    Authors: Thibault Barrit, Claire Campion, Sophie Aligon, Julie Bourbeillon, David Rousseau, Elisabeth Planchet and Béatrice Teulat
    Citation: Plant Methods 2022 18:131
  26. High-throughput phenotyping platforms allow the study of the form and function of a large number of genotypes subjected to different growing conditions (GxE). A number of image acquisition and processing pipel...

    Authors: Benoit Daviet, Romain Fernandez, Llorenç Cabrera-Bosquet, Christophe Pradal and Christian Fournier
    Citation: Plant Methods 2022 18:130
  27. The technology of cotton defoliation is essential for mechanical cotton harvesting. Agricultural unmanned aerial vehicle (UAV) spraying has the advantages of low cost, high efficiency and no mechanical damage ...

    Authors: Jinyong Wu, Sheng Wen, Yubin Lan, Xuanchun Yin, Jiantao Zhang and Yufeng Ge
    Citation: Plant Methods 2022 18:129
  28. As complete and accurate genome sequences are becoming easier to obtain, more researchers wish to get one or more of them to support their research endeavors. Reliable and well-documented sequence assembly wor...

    Authors: Marina Püpke Marone, Harmeet Chawla Singh, Curtis J. Pozniak and Martin Mascher
    Citation: Plant Methods 2022 18:128
  29. High-throughput phenotyping is crucial for the genetic and molecular understanding of adaptive root system development. In recent years, imaging automata have been developed to acquire the root system architec...

    Authors: Romain Fernandez, Amandine Crabos, Morgan Maillard, Philippe Nacry and Christophe Pradal
    Citation: Plant Methods 2022 18:127
  30. Our understanding of the physiological responses of rice inflorescence (panicle) to environmental stresses is limited by the challenge of accurately determining panicle photosynthetic parameters and their impa...

    Authors: Jaspinder Singh Dharni, Balpreet Kaur Dhatt, Puneet Paul, Tian Gao, Tala Awada, Harel Bacher, Zvi Peleg, Paul Staswick, Jason Hupp, Hongfeng Yu and Harkamal Walia
    Citation: Plant Methods 2022 18:126
  31. Grasses internodes are made of distinct tissues such as vascular bundles, epidermis, rind and pith. The histology of grasses stem was largely revisited recently taking advantage of the development of microscop...

    Authors: P.-L. Lopez-Marnet, S. Guillaume, V. Méchin and M. Reymond
    Citation: Plant Methods 2022 18:125
  32. Chlorophyll and carotenoids, the most widely distributed lipophilic pigments in plants, contribute to fruit coloration during development and ripening. These pigments are assembled with pigment-protein complex...

    Authors: Jinli Gong, Hang Zhang, Yunliu Zeng, Yunjiang Cheng, Xuepeng Sun and Pengwei Wang
    Citation: Plant Methods 2022 18:124
  33. Acidic phytohormones are small molecules controlling many physiological functions in plants. A comprehensive picture of their profiles including the active forms, precursors and metabolites provides an importa...

    Authors: Jitka Široká, Federica Brunoni, Aleš Pěnčík, Václav Mik, Asta Žukauskaitė, Miroslav Strnad, Ondřej Novák and Kristýna Floková
    Citation: Plant Methods 2022 18:122
  34. Commonly, several traits are assessed in agronomic experiments to better understand the factors under study. However, it is also common to see that even when several traits are available, researchers opt to fo...

    Authors: Tiago Olivoto, Maria I. Diel, Denise Schmidt and Alessandro D. Lúcio
    Citation: Plant Methods 2022 18:121
  35. Cytokinins are a class of phytohormone that play a crucial role in the development of plants. They are involved in the regulation of nearly every aspect of plant growth, from germination to senescence. The rol...

    Authors: Lachlan Dow, Russell A. Barrow, Rosemary G. White and Ulrike Mathesius
    Citation: Plant Methods 2022 18:120
  36. Phytochemicals or secondary metabolites are low molecular weight organic compounds with little function in plant growth and development. Nevertheless, the metabolite diversity govern not only the phenetics of ...

    Authors: Muhammad-Redha Abdullah-Zawawi, Nisha Govender, Mohammad Bozlul Karim, Md. Altaf-Ul-Amin, Shigehiko Kanaya and Zeti-Azura Mohamed-Hussein
    Citation: Plant Methods 2022 18:118
  37. Researchers interested in the seed size of rapeseed need to quantify the cell size and number of cells in the seed coat, embryo and silique wall. Scanning electron microscope-based methods have been demonstrat...

    Authors: Yushun Jiao, Baoling Liang, Guangsheng Yang, Qiang Xin and Dengfeng Hong
    Citation: Plant Methods 2022 18:117
  38. Soybean gene functions cannot be easily interrogated through transgenic disruption (knock-out) of genes-of-interest, or transgenic overexpression of proteins-of-interest, because soybean transformation is time...

    Authors: Fides Angeli Zaulda, Seung Hyun Yang, Junping Han, Sizolwenkosi Mlotshwa, Anne Dorrance and Feng Qu
    Citation: Plant Methods 2022 18:116
  39. Living cells maintain and adjust structural and functional integrity by continual synthesis and degradation of metabolites and macromolecules. The maintenance and adjustment of thylakoid membrane involve turno...

    Authors: Anh Thi-Mai Banh, Björn Thiele, Antonia Chlubek, Thomas Hombach, Einhard Kleist and Shizue Matsubara
    Citation: Plant Methods 2022 18:114

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