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  1. Optimum moisture in straw and grain at maturity is important for timely harvesting of wheat. Grain harvested at the right time has reduced chance of being affected by adverse weather conditions which is import...

    Authors: Jatinder S. Sangha, Brad Meyer, Yuefeng Ruan, Richard D. Cuthbert, Ron Knox and Gaozhi Xiao
    Citation: Plant Methods 2024 20:89
  2. To date, only a limited number of studies have utilized remote sensing imagery to estimate aboveground biomass (AGB) in the Miombo ecoregion using wall-to-wall medium resolution optical satellite imagery (Sent...

    Authors: Hastings Shamaoma, Paxie W. Chirwa, Jules C. Zekeng, Able Ramoelo, Andrew T. Hudak, Ferdinand Handavu and Stephen Syampungani
    Citation: Plant Methods 2024 20:88
  3. The selection of highly productive genotypes with stable performance across environments is a major challenge of plant breeding programs due to genotype-by-environment (GE) interactions. Over the years, differ...

    Authors: Humberto Fanelli Carvalho, Simon Rio, Julian García-Abadillo and Julio Isidro y Sánchez
    Citation: Plant Methods 2024 20:85
  4. PIN proteins establish the auxin concentration gradient, which coordinates plant growth. PIN1-4 and 7 localized at the plasma membrane (PM) and facilitate polar auxin transport while the endoplasmic reticulum ...

    Authors: Yewubnesh Wendimu Seifu, Vendula Pukyšová, Nikola Rýdza, Veronika Bilanovičová, Marta Zwiewka, Marek Sedláček and Tomasz Nodzyński
    Citation: Plant Methods 2024 20:84
  5. Fungal pathogens significantly impact the quality of fruits and vegetables at different stages of the supply chain, leading to substantial food losses. Understanding how these persistent fungal infections occu...

    Authors: Adrian O. Sbodio, Saskia D. Mesquida-Pesci, Nancy Yip, Isabela Alvarez-Rojo, Elia Gutierrez-Baeza, Samantha Tay, Pedro Bello, Luxin Wang and Barbara Blanco-Ulate
    Citation: Plant Methods 2024 20:83
  6. The process of optimizing in vitro shoot proliferation is a complicated task, as it is influenced by interactions of many factors as well as genotype. This study investigated the role of various concentrations...

    Authors: Saeedeh Zarbakhsh, Ali Reza Shahsavar and Mohammad Soltani
    Citation: Plant Methods 2024 20:82
  7. Plants are known to be infected by a wide range of pathogenic microbes. To study plant diseases caused by microbes, it is imperative to be able to monitor disease symptoms and microbial colonization in a quant...

    Authors: Misha Paauw, Gerrit Hardeman, Nanne W. Taks, Lennart Lambalk, Jeroen A. Berg, Sebastian Pfeilmeier and Harrold A. van den Burg
    Citation: Plant Methods 2024 20:80
  8. Plant transformation remains a major bottleneck to the improvement of plant science, both on fundamental and practical levels. The recalcitrant nature of most commercial and minor crops to genetic transformati...

    Authors: Jérôme Gélinas Bélanger, Tanya Rose Copley, Valerio Hoyos-Villegas, Jean-Benoit Charron and Louise O’Donoughue
    Citation: Plant Methods 2024 20:79
  9. Red raspberry fruit color is a key driver of consumer preference and a major target of breeding programs worldwide. Screening for fruit color typically involves the determination of anthocyanin content and/or ...

    Authors: Claudia Baldassi, Clover Lee, Michael Dossett and Simone D. Castellarin
    Citation: Plant Methods 2024 20:78
  10. Taraxacum kok-saghyz Rodin (TKS) is a highly potential source of natural rubber (NR) due to its wide range of suitable planting areas, strong adaptability, and suitability for mechanized planting and harvesting. ...

    Authors: Runfeng Chen, Qingqing Yan, Tuhanguli Tuoheti, Lin Xu, Qiang Gao, Yan Zhang, Hailong Ren, Lipeng Zheng, Feng Wang and Ya Liu
    Citation: Plant Methods 2024 20:77
  11. Isolated microspore culture is a useful biotechnological technique applied in modern plant breeding programs as it can produce doubled haploid (DH) plants and accelerate the development of new varieties. Furth...

    Authors: Runhong Gao, Yingjie Zong, Shuwei Zhang, Guimei Guo, Wenqi Zhang, Zhiwei Chen, Ruiju Lu, Chenghong Liu, Yifei Wang and Yingbo Li
    Citation: Plant Methods 2024 20:76
  12. The sweet potato whitefly (Bemisia tabaci) is a globally important insect pest that damages crops through direct feeding and by transmitting viruses. Current B. tabaci management revolves around the use of insect...

    Authors: Benjamin van Raalte, Kristal Watrous, Miguel Lujan, Ricky Le, Penglin Sun, Benjamin Ellis and Kerry E. Mauck
    Citation: Plant Methods 2024 20:75
  13. Fruit appearance of apple (Malus domestica Borkh.) is accession-specific and one of the main criteria for consumer choice. Consequently, fruit appearance is an important selection criterion in the breeding of new...

    Authors: Norbert Kirchgessner, Marius Hodel, Bruno Studer, Andrea Patocchi and Giovanni A. L. Broggini
    Citation: Plant Methods 2024 20:74
  14. X-ray computed tomography (CT) is a powerful tool for measuring plant root growth in soil. However, a rapid scan with larger pots, which is required for throughput-prioritized crop breeding, results in high no...

    Authors: Shota Teramoto and Yusaku Uga
    Citation: Plant Methods 2024 20:73
  15. The genetic basis of colour development in red-flesh apples (Malus domestica Borkh) has been widely characterised; however, current models do not explain the observed variations in red pigmentation intensity and ...

    Authors: Pierre Bouillon, Anne-Laure Fanciullino, Etienne Belin, Dimitri Bréard, Séverine Boisard, Béatrice Bonnet, Sylvain Hanteville, Frédéric Bernard and Jean-Marc Celton
    Citation: Plant Methods 2024 20:71
  16. Phytophthora sojae, a soil-borne oomycete pathogen, has been a yield limiting factor for more than 60 years on soybean. The resurgence of P. sojae (Phytophthora sojae) is primarily ascribed to the durable oospore...

    Authors: Xiaomeng Chu, Ziyi Yin, Pengjie Yue, Xinyu Wang, Yue Yang, Jiayi Sun, Ziying Kong, Jian Ren, Xiaohan Liu, Chongchong Lu, Haipeng Zhao, Yang Li and Xinhua Ding
    Citation: Plant Methods 2024 20:70
  17. Characterisation of the structure and water status of leaf tissues is essential to the understanding of leaf hydraulic functioning under optimal and stressed conditions. Magnetic Resonance Imaging is unique in...

    Authors: Pierre-Nicolas Boulc’h, Guylaine Collewet, Baptiste Guillon, Stéphane Quellec, Laurent Leport and Maja Musse
    Citation: Plant Methods 2024 20:69
  18. With the accumulating omics data, an efficient and time-saving transient assay to express target genes is desired. Mesophyll protoplasts, maintaining most stress-physiological responses and cellular activities...

    Authors: Shanshan Lei, Yaolong Zhu, Weiyu Jia, Jing Zhang, Yingjun Chi and Bin Xu
    Citation: Plant Methods 2024 20:67
  19. Citrus canker is a significant bacterial disease caused by Xanthomonas citri subsp. citri (Xcc) that severely impedes the healthy development of the citrus industry. Especially when citrus fruit is infected by Xc...

    Authors: Lian Liu, Xin Liu, Lingyi Liu, Tao Zhu, Rongchun Ye, Hao Chen, Linglei Zhou, Guang Wu, Limei Tan, Jian Han, Ronghua Li, Xianfeng Ma and Ziniu Deng
    Citation: Plant Methods 2024 20:65
  20. Cassava leaf samples degrade quickly during storage and transportation from distant areas. Proper sampling and efficient, low-cost storage methods are critical for obtaining sufficient quality DNA and RNA for ...

    Authors: Deogratius Mark, Fred Tairo, Joseph Ndunguru, Elisiana Kweka, Maliha Saggaf, Hilda Bachwenkizi, Evangelista Chiunga, James Leonard Lusana, Geofrey Sikazwe and Reuben Maghembe
    Citation: Plant Methods 2024 20:64
  21. The detection of internal defects in seeds via non-destructive imaging techniques is a topic of high interest to optimize the quality of seed lots. In this context, X-ray imaging is especially suited. Recent s...

    Authors: Sherif Hamdy, Aurélie Charrier, Laurence Le Corre, Pejman Rasti and David Rousseau
    Citation: Plant Methods 2024 20:63
  22. High-quality RNA extraction from woody plants is difficult because of the presence of polysaccharides and polyphenolics that bind or co-precipitate with the RNA. The CTAB (cetyl trimethylammonium bromide) base...

    Authors: Tibor Kiss, Zoltán Karácsony, Adrienn Gomba-Tóth, Kriszta Lilla Szabadi, Zsolt Spitzmüller, Júlia Hegyi-Kaló, Thomas Cels, Margot Otto, Richárd Golen, Ádám István Hegyi, József Geml and Kálmán Zoltán Váczy
    Citation: Plant Methods 2024 20:62
  23. Despite major efforts over the last decades, the rising demands of the growing global population makes it of paramount importance to increase crop yields and reduce losses caused by plant pathogens. One way to...

    Authors: Mackenzie Eli William Loranger, Winfield Yim, Vittorio Accomazzi, Nadia Morales-Lizcano, Wolfgang Moeder and Keiko Yoshioka
    Citation: Plant Methods 2024 20:60
  24. Artemisia campestris L. (AC) leaves are widely recognized for their importance in traditional medicine. Despite the considerable amount of research conducted on this plant overworld, the chemical composition and ...

    Authors: Inès Limam, Ridha Ghali, Mohamed Abdelkarim, Anis Ouni, Manel Araoud, Mouaadh Abdelkarim, Abderrazek Hedhili and Fatma Ben-Aissa Fennira
    Citation: Plant Methods 2024 20:59
  25. Traditional method of wood species identification involves the use of hand lens by wood anatomists, which is a time-consuming method that usually identifies only at the genetic level. Computer vision method ca...

    Authors: Chang Zheng, Shoujia Liu, Jiajun Wang, Yang Lu, Lingyu Ma, Lichao Jiao, Juan Guo, Yafang Yin and Tuo He
    Citation: Plant Methods 2024 20:56
  26. Improper management of agricultural and industrial cotton wastes causes environmental pollution and worsens the climate change challenge. Green recycling of cotton could contribute to a circular economy. One o...

    Authors: Mona A. Mohammed, Nagat M. Amer, Heba M. I. Abdallah and Mai S. Saleh
    Citation: Plant Methods 2024 20:54
  27. seeds that are recalcitrant to desiccation and freezing temperatures cannot be stored in gene banks under conventional conditions. However, the germplasm of some recalcitrant seeded species can be stored in li...

    Authors: Urszula Wasileńczyk, Mikołaj Krzysztof Wawrzyniak, João Paulo Rodrigues Martins, Paulina Kosek and Paweł Chmielarz
    Citation: Plant Methods 2024 20:53
  28. Salsola laricifolia is a typical C3–C4 typical desert plant, belonging to the family Amaranthaceae. An efficient single-cell system is crucial to study the gene function of this plant. In this study, we optimized...

    Authors: Hao Guo, Yuxin Xi, Kuerban Guzailinuer and Zhibin Wen
    Citation: Plant Methods 2024 20:52
  29. Stemphylium blight incited by Stemphylium botryosum poses a significant threat to lentil crops worldwide, inducing severe defoliation and causing substantial yield losses in susceptible varieties under favorable ...

    Authors: Mario González, Eleonora Barilli, Nicolas Rispail and Diego Rubiales
    Citation: Plant Methods 2024 20:51
  30. The root of a plant is a fundamental organ for the multisensory perception of the environment. Investigating root growth dynamics as a mean of their interaction with the environment is of key importance for im...

    Authors: Valentina Simonetti, Laura Ravazzolo, Benedetto Ruperti, Silvia Quaggiotti and Umberto Castiello
    Citation: Plant Methods 2024 20:50
  31. Leaf water content (LWC) significantly affects rice growth and development. Real-time monitoring of rice leaf water status is essential to obtain high yield and water use efficiency of rice plants with precise...

    Authors: Xuenan Zhang, Haocong Xu, Yehong She, Chao Hu, Tiezhong Zhu, Lele Wang, Liquan Wu, Cuicui You, Jian Ke, Qiangqiang Zhang and Haibing He
    Citation: Plant Methods 2024 20:48
  32. Cotton accounts for 80% of the global natural fibre production. Its leaf hairiness affects insect resistance, fibre yield, and economic value. However, this phenotype is still qualitatively assessed by visuall...

    Authors: Moshiur Farazi, Warren C. Conaty, Lucy Egan, Susan P. J. Thompson, Iain W. Wilson, Shiming Liu, Warwick N. Stiller, Lars Petersson and Vivien Rolland
    Citation: Plant Methods 2024 20:46
  33. For ten years, CRISPR/cas9 system has become a very useful tool for obtaining site-specific mutations on targeted genes in many plant organisms. This technology opens up a wide range of possibilities for impro...

    Authors: Jérémy Villette, Fatma Lecourieux, Eliot Bastiancig, Marie-Claire Héloir and Benoit Poinssot
    Citation: Plant Methods 2024 20:45
  34. Plant scientists have largely relied on pathogen growth assays and/or transcript analysis of stress-responsive genes for quantification of disease severity and susceptibility. These methods are destructive to ...

    Authors: Jae Hoon Lee, Unseok Lee, Ji Hye Yoo, Taek Sung Lee, Je Hyeong Jung and Hyoung Seok Kim
    Citation: Plant Methods 2024 20:44
  35. Dendrobium officinale is a medicinal plant with high commercial value. The Dendrobium officinale market in Yunnan is affected by the standardization of medicinal material quality control and the increase in marke...

    Authors: Peiyuan Li, Tao shen, Li Li and Yuanzhong Wang
    Citation: Plant Methods 2024 20:43
  36. Genomic selection (GS) has become an increasingly popular tool in plant breeding programs, propelled by declining genotyping costs, an increase in computational power, and rediscovery of the best linear unbias...

    Authors: Javier Fernández-González, Bertrand Haquin, Eliette Combes, Karine Bernard, Alix Allard and Julio Isidro y Sánchez
    Citation: Plant Methods 2024 20:42

    The Correction to this article has been published in Plant Methods 2024 20:66

  37. Gaseous phytohormone ethylene levels are directly influenced by the production of its immediate non-volatile precursor 1-aminocyclopropane-1-carboxylic acid (ACC). Owing to the strongly acidic character of the...

    Authors: Michal Karady, Pavel Hladík, Kateřina Cermanová, Petra Jiroutová, Ioanna Antoniadi, Rubén Casanova-Sáez, Karin Ljung and Ondřej Novák
    Citation: Plant Methods 2024 20:41
  38. Quantifying gene expression is a critical aspect of applied genomics research across all organisms, and real-time PCR has emerged as a powerful tool for this purpose. However, selecting appropriate internal co...

    Authors: Amin Ebrahimi, Shahrokh Gharanjik, Elham Azadvari and Sajad Rashidi-Monfared
    Citation: Plant Methods 2024 20:40

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