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  1. Content type: Methodology

    Climate change represents a grand challenge for agricultural productivity. Understanding complex plant traits such as stress tolerance, disease resistance or crop yield is thus essential for breeding and the d...

    Authors: Werner Jud, J. Barbro Winkler, Bishu Niederbacher, Simon Niederbacher and Jörg-Peter Schnitzler

    Citation: Plant Methods 2018 14:109

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  2. Content type: Methodology

    Genome editing of monocot plants can be accomplished by using the components of the CRISPR/Cas9 (clustered regularly interspaced short palindromic repeat/CRISPR associated Cas9) technology specifically optimiz...

    Authors: Sebastian Gasparis, Maciej Kała, Mateusz Przyborowski, Leszek A. Łyżnik, Wacław Orczyk and Anna Nadolska-Orczyk

    Citation: Plant Methods 2018 14:111

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  3. Content type: Methodology

    The mucilage is a model to study the polysaccharide biosynthesis since it is produced in large amounts and composed of complex polymers. In addition, it is of great economic interest for its technical and nutr...

    Authors: Fabien Miart, Jean-Xavier Fontaine, Christophe Pineau, Hervé Demailly, Brigitte Thomasset, Olivier Van Wuytswinkel, Karine Pageau and François Mesnard

    Citation: Plant Methods 2018 14:112

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  4. Content type: Research

    Real-time quantitative PCR has been widely used as the most reliable method to measure gene expression, due to its high accuracy and specificity. Wild barley (Hordeum brevisubulatum (Trin.) Link) is a wild relati...

    Authors: Lili Zhang, Qike Zhang, Ying Jiang, Yang Li, Haiwen Zhang and Ruifen Li

    Citation: Plant Methods 2018 14:110

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  5. Content type: Methodology

    The phenological development of the maize crop from emergence through flowering to maturity, usually expressed as a rate (i.e. 1/duration), is largely controlled by temperature in the tropics. Maize plant phen...

    Authors: Henri E. Z. Tonnang, Dan Makumbi and Peter Craufurd

    Citation: Plant Methods 2018 14:108

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  6. Content type: Methodology

    Protein N-glycosylation is initiated within the endoplasmic reticulum through the synthesis of a lipid-linked oligosaccharides (LLO) precursor. This precursor is then transferred en bloc on neo-synthesized protei...

    Authors: Pierre-Louis Lucas, Rodolphe Dumontier, Corinne Loutelier-Bourhis, Alain Mareck, Carlos Afonso, Patrice Lerouge, Narimane Mati-Baouche and Muriel Bardor

    Citation: Plant Methods 2018 14:107

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  7. Content type: Correction

    In the original version of this article [1], the spelling of the tenth author name, Moakai Yan, was incorrect. The corrected name is given in this correction article.

    Authors: S. V. G. N. Priyadarshani, Bingyan Hu, Weimin Li, Hina Ali, Haifeng Jia, Lihua Zhao, Simon Peter Ojolo, Syed Muhammad Azam, Junjie Xiong, Maokai Yan, Zia ur Rahman, Qingsong Wu and Yuan Qin

    Citation: Plant Methods 2018 14:106

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    The original article was published in Plant Methods 2018 14:95

  8. Content type: Methodology

    Ability to measure ethylene is an important aspect of postharvest management, as knowledge of endogenous ethylene production is used in assessing physiological status, while response of crops to exogenous ethy...

    Authors: Sunny George Gwanpua, Abdul Jabbar, Jeritah Tongonya, Sue Nicholson and Andrew R. East

    Citation: Plant Methods 2018 14:105

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  9. Content type: Methodology

    Abscisic acid (ABA) functions as a stress phytohormone in many growth and developmental processes in plants. The ultra-sensitive determination of ABA would help to better understand its vital roles and action ...

    Authors: Yi Su, Wei Li, Zhigang Huang, Ruozhong Wang, Weigui Luo, Qing Liu, Jianhua Tong and Langtao Xiao

    Citation: Plant Methods 2018 14:104

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  10. Content type: Research

    Fungal pathogens like Fusarium graminearum can cause severe yield losses and mycotoxin contamination of food and feed worldwide. We recently showed its ability to systemically colonize wheat via root infection. H...

    Authors: Dhaka Ram Bhandari, Qing Wang, Bin Li, Wolfgang Friedt, Andreas Römpp, Bernhard Spengler and Sven Gottwald

    Citation: Plant Methods 2018 14:103

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  11. Content type: Research

    To investigate plant hybrid sterility, we studied interspecific hybrids of two cultivated rice species, Asian rice (Oryza sativa) and African rice (O. glaberrima). Male gametes of these hybrids display complete s...

    Authors: Yoshitaka Kanaoka, Daichi Kuniyoshi, Eri Inada, Yohei Koide, Yoshihiro Okamoto, Hideshi Yasui and Yuji Kishima

    Citation: Plant Methods 2018 14:102

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  12. Content type: Research

    The number of cultivated wheat seedlings per unit area allows calculation of plant density. Wheat seedling density provides emergence data and this is useful for improving crop management. The number of wheat ...

    Authors: Tao Liu, Tianle Yang, Chunyan Li, Rui Li, Wei Wu, Xiaochun Zhong, Chengming Sun and Wenshan Guo

    Citation: Plant Methods 2018 14:101

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  13. Content type: Methodology

    Leaf cellular architecture plays an important role in setting limits for carbon assimilation and, thus, photosynthetic performance. However, the low density, fine structure, and sensitivity to desiccation of p...

    Authors: Andrew W. Mathers, Christopher Hepworth, Alice L. Baillie, Jen Sloan, Hannah Jones, Marjorie Lundgren, Andrew J. Fleming, Sacha J. Mooney and Craig J. Sturrock

    Citation: Plant Methods 2018 14:99

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  14. Content type: Research

    Expression of economically relevant proteins in alternative expression platforms, especially plant expression platforms, has gained significant interest in recent years. A special interest in working with plan...

    Authors: Israel A. Ramírez-Alanis, Justin B. Renaud, Silverio García-Lara, Rima Menassa and Guy A. Cardineau

    Citation: Plant Methods 2018 14:98

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  15. Content type: Research

    Improving plant water use efficiency (WUE) is a major target for improving crop yield resilience to adverse climate change. Identifying genetic variation in WUE usually relies on instantaneous measurements of ...

    Authors: Iván Jauregui, Shane A. Rothwell, Samuel H. Taylor, Martin A. J. Parry, Elizabete Carmo-Silva and Ian C. Dodd

    Citation: Plant Methods 2018 14:97

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  16. Content type: Research

    An efficient transformation protocol is a primary requisite to study and utilize the genetic potential of any plant species. A quick transformation system is also crucial for the functional analysis of genes a...

    Authors: S. V. G. N. Priyadarshani, Bingyan Hu, Weimin Li, Hina Ali, Haifeng Jia, Lihua Zhao, Simon Peter Ojolo, Syed Muhammad Azam, Junjie Xiong, Maokai Yan, Zia ur Rahman, Qingsong Wu and Yuan Qin

    Citation: Plant Methods 2018 14:95

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    The Correction to this article has been published in Plant Methods 2018 14:106

  17. Content type: Methodology

    The clear visualization of 3D organization at the cellular level in plant tissues is needed to fully understand plant development processes. Imaging tools allow the visualization of the main fluorophores and i...

    Authors: Charlotte Bureau, Nadège Lanau, Mathieu Ingouff, Boukhaddaoui Hassan, Anne-Cécile Meunier, Fanchon Divol, Rosie Sevilla, Delphine Mieulet, Anne Dievart and Christophe Périn

    Citation: Plant Methods 2018 14:96

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  18. Content type: Methodology

    The reduced growth of plants during the winter causes a lack in the perceptibility of the phenological events making challenging the study of dormancy. For deciduous crops, dormancy is generally determined by ...

    Authors: Hector Camargo Alvarez, Melba Salazar-Gutiérrez, Diana Zapata, Markus Keller and Gerrit Hoogenboom

    Citation: Plant Methods 2018 14:94

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  19. Content type: Methodology

    Genetic transformation is a valuable tool and an important procedure in plant functional genomics contributing to gene discovery, allowing powerful insights into gene function and genetically controlled charac...

    Authors: Sadiye Hayta, Mark A. Smedley, Jinhong Li, Wendy A. Harwood and Philip M. Gilmartin

    Citation: Plant Methods 2018 14:93

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  20. Content type: Methodology

    Plasmopara obducens is the biotrophic oomycete responsible for impatiens downy mildew, a destructive disease of Impatiens that causes high crop loss. Currently, there are no available methods for the microscopic ...

    Authors: Catalina Salgado-Salazar, Gary R. Bauchan, Emma C. Wallace and Jo Anne Crouch

    Citation: Plant Methods 2018 14:92

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  21. Content type: Methodology

    The in vivo observation of diffusible components, such as ions and small phenolic compounds, remains a challenge in turgid plant organs. The analytical techniques used to localize such components in water-rich...

    Authors: Kevin Vidot, Cédric Gaillard, Camille Rivard, René Siret and Marc Lahaye

    Citation: Plant Methods 2018 14:89

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  22. Content type: Research

    Due to cowpea ability to fix nitrogen in poor soils and relative tolerance to drought and salt stresses, efforts have been directed to identifying genes and pathways that confer stress tolerance in this specie...

    Authors: Lidiane Lindinalva Barbosa Amorim, José Ribamar Costa Ferreira-Neto, João Pacífico Bezerra-Neto, Valesca Pandolfi, Flávia Tadeu de Araújo, Mitalle Karen da Silva Matos, Mauro Guida Santos, Ederson Akio Kido and Ana Maria Benko-Iseppon

    Citation: Plant Methods 2018 14:88

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  23. Content type: Review

    Production of virus-free plants is necessary to control viral diseases, import novel cultivars from other countries, exchange breeding materials between countries or regions and preserve plant germplasm. In vi...

    Authors: Min-Rui Wang, Zhen-Hua Cui, Jing-Wei Li, Xin-Yi Hao, Lei Zhao and Qiao-Chun Wang

    Citation: Plant Methods 2018 14:87

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  24. Content type: Research

    Charcoal rot is a fungal disease that thrives in warm dry conditions and affects the yield of soybeans and other important agronomic crops worldwide. There is a need for robust, automatic and consistent early ...

    Authors: Koushik Nagasubramanian, Sarah Jones, Soumik Sarkar, Asheesh K. Singh, Arti Singh and Baskar Ganapathysubramanian

    Citation: Plant Methods 2018 14:86

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  25. Content type: Methodology

    Root phenotyping aims to characterize root system architecture because of its functional role in resource acquisition. RGB imaging and analysis procedures measure root system traits via colour contrasts betwee...

    Authors: Gernot Bodner, Alireza Nakhforoosh, Thomas Arnold and Daniel Leitner

    Citation: Plant Methods 2018 14:84

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  26. Content type: Methodology

    When developing CRISPR/Cas9 systems for crops, it is crucial to invest time characterizing the genome editing efficiency of the CRISPR/Cas9 cassettes, especially if the transformation system is difficult or ti...

    Authors: Lu Long, Dan-Dan Guo, Wei Gao, Wen-Wen Yang, Li-Pan Hou, Xiao-Nan Ma, Yu-Chen Miao, Jose Ramon Botella and Chun-Peng Song

    Citation: Plant Methods 2018 14:85

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  27. Content type: Research

    Recently, putative pre-miRNAs locations have been identified in the introns of plant genes, raising the question whether such genes can show a dual functionality by having both correct maturation of the host g...

    Authors: Umidjon Shapulatov, Mark van Hoogdalem, Marielle Schreuder, Harro Bouwmeester, Ibrokhim Y. Abdurakhmonov and Alexander R. van der Krol

    Citation: Plant Methods 2018 14:83

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  28. Content type: Methodology

    The paper introduces a multispectral imaging system and data-processing approach for the identification and discrimination of morphologically indistinguishable cryptic species of the destructive crop pest, the...

    Authors: Joseph Fennell, Charles Veys, Jose Dingle, Joachim Nwezeobi, Sharon van Brunschot, John Colvin and Bruce Grieve

    Citation: Plant Methods 2018 14:82

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  29. Content type: Methodology

    Existing methods for directly measuring photosynthetic capacity (Amax) have low throughput, which creates a key bottleneck for pre-breeding and ecological research. Currently available commercial leaf gas exchang...

    Authors: William T. Salter, Matthew E. Gilbert and Thomas N. Buckley

    Citation: Plant Methods 2018 14:80

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  30. Content type: Research

    Thapsigargin and nortrilobolide are sesquiterpene lactones found in the Mediterranean plant Thapsia garganica L. Thapsigargin is a potent inhibitor of the sarco/endoplasmic reticulum calcium ATPase pump, inducing...

    Authors: Carmen Quiñonero López, Patricia Corral, Bénédicte Lorrain-Lorrette, Karen Martinez-Swatson, Franck Michoux and Henrik Toft Simonsen

    Citation: Plant Methods 2018 14:79

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  31. Content type: Software

    Progress in high-throughput molecular methods accompanied by more complex experimental designs demands novel data visualisation solutions. To specifically answer the question which parts of the specifical biol...

    Authors: Maja Zagorščak, Andrej Blejec, Živa Ramšak, Marko Petek, Tjaša Stare and Kristina Gruden

    Citation: Plant Methods 2018 14:78

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  32. Content type: Research

    The visible and near infrared region has been widely used to estimate the leaf nitrogen (N) content based on the correlation of N with chlorophyll and deep absorption valleys of chlorophyll in this region. How...

    Authors: Dong Li, Xue Wang, Hengbiao Zheng, Kai Zhou, Xia Yao, Yongchao Tian, Yan Zhu, Weixing Cao and Tao Cheng

    Citation: Plant Methods 2018 14:76

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  33. Content type: Methodology

    Wheat (Triticum aestivum L.) productivity is commonly limited by the availability of water. Increasing transpiration efficiency (biomass produced per unit of water used, TE) can potentially lead to increased grai...

    Authors: Andrew Fletcher, Jack Christopher, Mal Hunter, Greg Rebetzke and Karine Chenu

    Citation: Plant Methods 2018 14:77

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  34. Content type: Methodology

    The recent development of next-generation sequencing DNA marker technologies, such as genotyping-by-sequencing (GBS), generates thousands of informative single nucleotide polymorphism markers in almost any spe...

    Authors: Craig B. Anderson, Benjamin K. Franzmayr, Soon Won Hong, Anna C. Larking, Tracey C. van Stijn, Rachel Tan, Roger Moraga, Marty J. Faville and Andrew G. Griffiths

    Citation: Plant Methods 2018 14:75

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  35. Content type: Methodology

    We report the construction of a Virus-Induced Gene Silencing (VIGS) vector and an agroinoculation protocol for gene silencing in cassava (Manihot esculenta Crantz) leaves and roots. The African cassava mosaic vir...

    Authors: Ezequiel Matias Lentz, Joel-Elias Kuon, Adrian Alder, Nathalie Mangel, Ima M. Zainuddin, Emily Jane McCallum, Ravi Bodampalli Anjanappa, Wilhelm Gruissem and Hervé Vanderschuren

    Citation: Plant Methods 2018 14:73

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  36. Content type: Research

    Lignocellulosic biomass is a complex network of polymers making the cell walls of plants. It represents a feedstock of sustainable resources to be converted into fuels, chemicals and materials. Because of its ...

    Authors: Christine Terryn, Gabriel Paës and Corentin Spriet

    Citation: Plant Methods 2018 14:74

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  37. Content type: Research

    Sample preparation is a critical process for proteomic studies. Many efficient and reproducible sample preparation methods have been developed for mass spectrometry-based proteomic analysis of human and animal...

    Authors: Wei-Qing Wang, Ole Nørregaard Jensen, Ian Max Møller, Kim H. Hebelstrup and Adelina Rogowska-Wrzesinska

    Citation: Plant Methods 2018 14:72

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  38. Content type: Methodology

    The next-generation sequencing (NGS) technology has greatly facilitated genomic and transcriptomic studies, contributing significantly in expanding the current knowledge on genome and transcriptome. However, t...

    Authors: Fu-Yuan Zhu, Mo-Xian Chen, Neng-Hui Ye, Wang-Min Qiao, Bei Gao, Wai-Ki Law, Yuan Tian, Dong Zhang, Di Zhang, Tie-Yuan Liu, Qi-Juan Hu, Yun-Ying Cao, Ze-Zhuo Su, Jianhua Zhang and Ying-Gao Liu

    Citation: Plant Methods 2018 14:69

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  39. Content type: Methodology

    Full scent profiles emitted by living tissues can be screened by using total ion chromatograms generated in full scan mode and gas chromatography–mass spectrometry technique using Headspace Sorptive Extraction...

    Authors: Victoria Ruiz-Hernández, María José Roca, Marcos Egea-Cortines and Julia Weiss

    Citation: Plant Methods 2018 14:67

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  40. Content type: Methodology

    High resolution and high throughput genotype to phenotype studies in plants are underway to accelerate breeding of climate ready crops. In the recent years, deep learning techniques and in particular Convoluti...

    Authors: Sarah Taghavi Namin, Mohammad Esmaeilzadeh, Mohammad Najafi, Tim B. Brown and Justin O. Borevitz

    Citation: Plant Methods 2018 14:66

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  41. Content type: Research

    Our knowledge of natural genetic variation is increasing at an extremely rapid pace, affording an opportunity to come to a much richer understanding of how effects of specific genes are dependent on the geneti...

    Authors: Rui Wu, Miriam Lucke, Yun-ting Jang, Wangsheng Zhu, Efthymia Symeonidi, Congmao Wang, Joffrey Fitz, Wanyan Xi, Rebecca Schwab and Detlef Weigel

    Citation: Plant Methods 2018 14:65

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  42. Content type: Research

    The images of different flower species had small inter-class variations across different classes as well as large intra-class variations within a class. Flower classification techniques are mainly based on the...

    Authors: Ping Lin, Du Li, Zhiyong Zou, Yongming Chen and Shanchao Jiang

    Citation: Plant Methods 2018 14:64

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  43. Content type: Methodology

    The model species Arabidopsis thaliana has extensive resources to investigate intraspecific trait variability and the genetic bases of ecologically relevant traits. However, the cost of equipment and software req...

    Authors: François Vasseur, Justine Bresson, George Wang, Rebecca Schwab and Detlef Weigel

    Citation: Plant Methods 2018 14:63

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