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  1. Tocochromanols are a group of lipid-soluble antioxidants produced by all plants and include the tocopherols, which are ubiquitous in the plant kingdom, and the biosynthetically-related compounds tocotrienols, ...

    Authors: Yan Bao, Maria Magallenes-Lundback, Nicholas Deason and Dean DellaPenna

    Citation: Plant Methods 2020 16:126

    Content type: Methodology

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  2. Strigolactones represent the most recently described group of plant hormones involved in many aspects of plant growth regulation. Simultaneously, root exuded strigolactones mediate rhizosphere signaling toward...

    Authors: Kristýna Floková, Mahdere Shimels, Beatriz Andreo Jimenez, Nicoletta Bardaro, Miroslav Strnad, Ondřej Novák and Harro J. Bouwmeester

    Citation: Plant Methods 2020 16:125

    Content type: Methodology

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  3. The rising availability of assemblies of large genomes (e.g. bread and durum wheat, barley) and their annotations deliver the basis to graphically present genome organization of parents and progenies on a phys...

    Authors: Mathieu Deblieck, Andrii Fatiukha, Norbert Grundman, Lianne Merchuk-Ovnat, Yehoshua Saranga, Tamar Krugman, Klaus Pillen, Albrecht Serfling, Wojciech Makalowski, Frank Ordon and Dragan Perovic

    Citation: Plant Methods 2020 16:123

    Content type: Software

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  4. Up-to-now, several biochemical methods have been developed to allow specific organelle isolation from plant tissues. These procedures are often time consuming, require substantial amounts of plant material, ha...

    Authors: Martina Lang, Marlene Pröschel, Nico Brüggen and Uwe Sonnewald

    Citation: Plant Methods 2020 16:122

    Content type: Methodology

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  5. Genomic methods for identifying causative variants for trait loci applicable to a wide range of germplasm are required for plant biologists and breeders to understand the genetic control of trait variation.

    Authors: Elena López-Girona, Marcus W. Davy, Nick W. Albert, Elena Hilario, Maia E. M. Smart, Chris Kirk, Susan J. Thomson and David Chagné

    Citation: Plant Methods 2020 16:121

    Content type: Methodology

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  6. Chloroplast genome information is critical to understanding forms of photosynthesis in the plant kingdom. During the evolutionary process, plants have developed different photosynthetic strategies that are acc...

    Authors: Richard M. Sharpe, Bruce Williamson-Benavides, Gerald E. Edwards and Amit Dhingra

    Citation: Plant Methods 2020 16:119

    Content type: Research

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  7. Measurement of plant structure is useful in monitoring plant conditions and understanding the responses of plants to environmental changes. 3D imaging technologies, especially the passive-SfM (Structure from M...

    Authors: Xingtong Lu, Eiichi Ono, Shan Lu, Yu Zhang, Poching Teng, Mitsuko Aono, Yo Shimizu, Fumiki Hosoi and Kenji Omasa

    Citation: Plant Methods 2020 16:118

    Content type: Research

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  8. Identification and characterization of new traits with sound physiological foundation is essential for crop breeding and production management. Deep learning has been widely used in image data analysis to expl...

    Authors: Zongfeng Yang, Shang Gao, Feng Xiao, Ganghua Li, Yangfeng Ding, Qinghua Guo, Matthew J. Paul and Zhenghui Liu

    Citation: Plant Methods 2020 16:117

    Content type: Research

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  9. Walnuts are grown worldwide in temperate areas and producers are facing an increasing demand. In a climate change context, the industry also needs cultivars that provide fruits of quality. This quality include...

    Authors: Anthony Bernard, Sherif Hamdy, Laurence Le Corre, Elisabeth Dirlewanger and Fabrice Lheureux

    Citation: Plant Methods 2020 16:115

    Content type: Research

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  10. The black pod disease affects cacao plantations worldwide; it is caused by the oomycete species of the genus Phytophthora. The resistance of cacao plants to the black pod is commonly evaluated by artificial inocu...

    Authors: Paola Delgadillo-Durán, Mauricio Soto-Suárez, Leonora Rodriguez-Polanco, Martha Carrero-Gutierrez, Esperanza Torres-Rojas and Roxana Yockteng

    Citation: Plant Methods 2020 16:114

    Content type: Methodology

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  11. Stalk lodging (breaking of agricultural plant stalks prior to harvest) is a multi-billion dollar a year problem. Rind penetration resistance tests have been used by plant scientists and breeders to estimate th...

    Authors: Christopher J. Stubbs, Christopher McMahan, Will Seegmiller, Douglas D. Cook and Daniel J. Robertson

    Citation: Plant Methods 2020 16:113

    Content type: Research

    Published on:

  12. Optimizing the somatic embryogenesis protocol can be considered as the first and foremost step in successful gene transformation studies. However, it is usually difficult to achieve an optimized embryogenesis ...

    Authors: Mohsen Hesami, Roohangiz Naderi, Masoud Tohidfar and Mohsen Yoosefzadeh-Najafabadi

    Citation: Plant Methods 2020 16:112

    Content type: Research

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  13. Sowing time is commonly used as the temporal reference for Arabidopsis thaliana (Arabidopsis) experiments in high throughput plant phenotyping (HTPP) systems. This relies on the assumption that germination and se...

    Authors: Diego Lozano-Claros, Xiangxiang Meng, Eddie Custovic, Guang Deng, Oliver Berkowitz, James Whelan and Mathew G. Lewsey

    Citation: Plant Methods 2020 16:111

    Content type: Methodology

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  14. The use of sequencing and genotyping platforms has undergone dramatic improvements, enabling the generation of a wealth of genomic information. Despite this progress, the availability of high-quality genomic D...

    Authors: Santiago Vilanova, David Alonso, Pietro Gramazio, Mariola Plazas, Edgar García-Fortea, Paola Ferrante, Maximilian Schmidt, María José Díez, Björn Usadel, Giovanni Giuliano and Jaime Prohens

    Citation: Plant Methods 2020 16:110

    Content type: Methodology

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  15. Photosynthesis is one of the most important biological reactions and forms the basis of crop productivity and yield on which a growing global population relies. However, to develop improved plant cultivars tha...

    Authors: Matthew T. Herritt, Duke Pauli, Todd C. Mockler and Alison L. Thompson

    Citation: Plant Methods 2020 16:109

    Content type: Methodology

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  16. The population of plants is a crucial indicator in plant phenotyping and agricultural production, such as growth status monitoring, yield estimation, and grain depot management. To enhance the production effic...

    Authors: Hongwei Zou, Hao Lu, Yanan Li, Liang Liu and Zhiguo Cao

    Citation: Plant Methods 2020 16:108

    Content type: Database

    Published on:

  17. Phenotypic information is of great significance for irrigation management, disease prevention and yield improvement. Interest in the evaluation of phenotypes has grown with the goal of enhancing the quality of...

    Authors: Yirui Huang, Zhenhui Ren, Dongming Li and Xuan Liu

    Citation: Plant Methods 2020 16:107

    Content type: Review

    Published on:

  18. Wheat yield is influenced by the number of ears per unit area, and manual counting has traditionally been used to estimate wheat yield. To realize rapid and accurate wheat ear counting, K-means clustering was ...

    Authors: Xin Xu, Haiyang Li, Fei Yin, Lei Xi, Hongbo Qiao, Zhaowu Ma, Shuaijie Shen, Binchao Jiang and Xinming Ma

    Citation: Plant Methods 2020 16:106

    Content type: Research

    Published on:

  19. Earth Observation ‘EO’ remote sensing technology development enables original insights into vegetation function and health at ever finer temporal, spectral and spatial resolution. Research sites equipped with ...

    Authors: William Woodgate, Eva van Gorsel, Dale Hughes, Lola Suarez, Jose Jimenez-Berni and Alex Held

    Citation: Plant Methods 2020 16:105

    Content type: Methodology

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  20. Timely and accurate estimates of canopy chlorophyll (Chl) a and b content are crucial for crop growth monitoring and agricultural management. Crop canopy reflectance depends on many factors, which can be divid...

    Authors: Jibo Yue, Haikuan Feng, Qingjiu Tian and Chengquan Zhou

    Citation: Plant Methods 2020 16:104

    Content type: Research

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  21. Monitoring the timing of seedling emergence and early development via high-throughput phenotyping with computer vision is a challenging topic of high interest in plant science. While most studies focus on the ...

    Authors: Salma Samiei, Pejman Rasti, Joseph Ly Vu, Julia Buitink and David Rousseau

    Citation: Plant Methods 2020 16:103

    Content type: Research

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  22. Ericoid mycorrhizal fungi (EMF) play important roles in mineral cycling and plant nutrient acquisition, and they increase plant survival in nutrient-poor environments. In this study, we detected the colonizati...

    Authors: Hongyi Yang, Xingyu Zhao, Lili Li and Jie Zhang

    Citation: Plant Methods 2020 16:102

    Content type: Research

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  23. Maize is one of the most important cereal crop all over the world with a complex genome of about 2.3 gigabase, and exhibits tremendous phenotypic and molecular diversity among different germplasms. Along with ...

    Authors: Shuaiqiang Liang, Feng Lin, Yiliang Qian, Tifu Zhang, Yibo Wu, Yaocheng Qi, Sihai Ren, Long Ruan and Han Zhao

    Citation: Plant Methods 2020 16:101

    Content type: Research

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  24. The increasing number of novel approaches for large-scale, multi-dimensional imaging of cells has created an unprecedented opportunity to analyze plant morphogenesis. However, complex image processing, includi...

    Authors: Xi Zhang, Zijian Hu, Yayu Guo, Xiaoyi Shan, Xiaojuan Li and Jinxing Lin

    Citation: Plant Methods 2020 16:100

    Content type: Methodology

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  25. Identification and characterization of key enzymes associated with cell wall biosynthesis and modification is fundamental to gain insights into cell wall dynamics. However, it is a challenge that activity assa...

    Authors: Wanchen Shao, Rita Sharma, Mads H. Clausen and Henrik V. Scheller

    Citation: Plant Methods 2020 16:99

    Content type: Methodology

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  26. In 2005, researchers from the French National Research Institute for Agriculture, Food and Environment (Institut national de recherche pour l’agriculture, l’alimentation et l’environnement, INRAE) started a co...

    Authors: Yannick De Oliveira, Laura Burlot, Julie C. Dawson, Isabelle Goldringer, Darkawi Madi, Pierre Rivière, Delphine Steinbach, Gaëlle van Frank and Mathieu Thomas

    Citation: Plant Methods 2020 16:98

    Content type: Software

    Published on:

  27. Field-based high-throughput plant phenotyping (FB-HTPP) has been a primary focus for crop improvement to meet the demands of a growing population in a changing environment. Over the years, breeders, geneticist...

    Authors: Alison L. Thompson, Kelly R. Thorp, Matthew M. Conley, Michael Roybal, David Moller and Jacob C. Long

    Citation: Plant Methods 2020 16:97

    Content type: Review

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  28. Endosperm-trait related genes are associated with grain yield or quality in maize. There are vast numbers of these genes whose functions and regulations are still unknown. The biolistic system, which is often ...

    Authors: Yufeng Hu, Dalin Song, Lei Gao, Babatope Samuel Ajayo, Yongbin Wang, Huanhuan Huang, Junjie Zhang, Hanmei Liu, Yinghong Liu, Guowu Yu, Yongjian Liu, Yangping Li and Yubi Huang

    Citation: Plant Methods 2020 16:96

    Content type: Methodology

    Published on:

  29. Automated segmentation of large amount of image data is one of the major bottlenecks in high-throughput plant phenotyping. Dynamic optical appearance of developing plants, inhomogeneous scene illumination, sha...

    Authors: Michael Henke, Astrid Junker, Kerstin Neumann, Thomas Altmann and Evgeny Gladilin

    Citation: Plant Methods 2020 16:95

    Content type: Methodology

    Published on:

  30. Agrobacterium rhizogenes-mediated hairy root transformation provides a powerful tool for investigating the functions of plant genes involved in rhizobia-legume symbiosis. However, in the traditional identificatio...

    Authors: Yinglun Fan, Xiuyuan Wang, Haiyun Li, Shuang Liu, Liangshen Jin, Yanyan Lyu, Mengdi Shi, Sirui Liu, Xinyue Yang and Shanhua Lyu

    Citation: Plant Methods 2020 16:94

    Content type: Methodology

    Published on:

  31. Genetically encoded fluorescent proteins are often used to label proteins and study protein function and localization in vivo. Traditional cloning methods mediated by restriction digestion and ligation are tim...

    Authors: Liu Zhang, Yang Zhao, Haiyan Liang, Xugang Li, Kimberly L. Gallagher and Shuang Wu

    Citation: Plant Methods 2020 16:93

    Content type: Methodology

    Published on:

  32. Photosynthesis of reproductive organs in C3 cereals is generally regarded as important to crop yield. Whereas, photosynthetic characteristics of reproductive organs are much less understood as compared to leaf ph...

    Authors: Tian-Gen Chang, Qing-Feng Song, Hong-Long Zhao, Shuoqi Chang, Changpeng Xin, Mingnan Qu and Xin-Guang Zhu

    Citation: Plant Methods 2020 16:92

    Content type: Methodology

    Published on:

  33. The knowledge of vine vegetative status within a vineyard plays a key role in canopy management in order to achieve a correct vine balance and reach the final desired yield/quality. Detailed information about ...

    Authors: Salvatore Filippo Di Gennaro and Alessandro Matese

    Citation: Plant Methods 2020 16:91

    Content type: Methodology

    Published on:

  34. Deeper roots help plants take up available resources in deep soil ensuring better growth and higher yields under conditions of drought. A large-scale semi-field root phenotyping facility was developed to allow...

    Authors: Xiangyu Guo, Simon F. Svane, Winnie S. Füchtbauer, Jeppe R. Andersen, Just Jensen and Kristian Thorup-Kristensen

    Citation: Plant Methods 2020 16:90

    Content type: Research

    Published on:

  35. Root system architecture and especially its plasticity in acclimation to variable environments play a crucial role in the ability of plants to explore and acquire efficiently soil resources and ensure plant pr...

    Authors: Kerstin A. Nagel, Henning Lenz, Bernd Kastenholz, Frank Gilmer, Andreas Averesch, Alexander Putz, Kathrin Heinz, Andreas Fischbach, Hanno Scharr, Fabio Fiorani, Achim Walter and Ulrich Schurr

    Citation: Plant Methods 2020 16:89

    Content type: Methodology

    Published on:

  36. The legume cowpea (Vigna unguiculata L.) is extensively grown in sub-Saharan Africa. Cowpea, like many legumes has proved recalcitrant to plant transformation. A rapid transient leaf assay was developed for testi...

    Authors: Martina Juranić, Dilrukshi S. K. Nagahatenna, Rigel Salinas-Gamboa, Melanie L. Hand, Nidia Sánchez-León, Weng Herng Leong, Tracy How, Natalia Bazanova, Andrew Spriggs, Jean-Philippe Vielle-Calzada and Anna M. G. Koltunow

    Citation: Plant Methods 2020 16:88

    Content type: Methodology

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  37. Rapid non-destructive measurements to predict cassava root yield over the full growing season through large numbers of germplasm and multiple environments is a huge challenge in Cassava breeding programs. As o...

    Authors: Michael Gomez Selvaraj, Manuel Valderrama, Diego Guzman, Milton Valencia, Henry Ruiz and Animesh Acharjee

    Citation: Plant Methods 2020 16:87

    Content type: Research

    Published on:

  38. Plant discrimination is of relevance for taxonomic, evolutionary, breeding and nutritional studies. To this purpose, evidence is reported to demonstrate TBP (Tubulin-Based-Polymorphism) as a DNA-based method s...

    Authors: Luca Braglia, Floriana Gavazzi, Laura Morello, Silvia Gianì, Peter Nick and Diego Breviario

    Citation: Plant Methods 2020 16:86

    Content type: Research

    Published on:

  39. The number of studies using third-generation sequencing utilising Pacific Biosciences (PacBio) and Oxford Nanopore Technologies (ONT) is rapidly increasing in many different research areas. Among them, plant f...

    Authors: Jiawen Cui, Nan shen, Zhaogeng Lu, Guolu Xu, Yuyao Wang and Biao Jin

    Citation: Plant Methods 2020 16:85

    Content type: Methodology

    Published on:

  40. Ingrowth-core method is a useful tool to determine fine root growth of standing crops by inserting root-free soil in mesh-bags for certain period of time. However, the root density observed by the method does ...

    Authors: Eusun Han, Dorte Bodin Dresbøll and Kristian Thorup-Kristensen

    Citation: Plant Methods 2020 16:84

    Content type: Methodology

    Published on:

  41. Many metals are essential for plants and humans. Knowledge of metal distribution in plant tissues in vivo contributes to the understanding of physiological mechanisms of metal uptake, accumulation and sequestr...

    Authors: Ana Mijovilovich, Filis Morina, Syed Nadeem Bokhari, Timo Wolff and Hendrik Küpper

    Citation: Plant Methods 2020 16:82

    Content type: Methodology

    Published on:

  42. Generation of marker-free transgenic plants is very important to the regulatory permission and commercial release of transgenic crops. Co-transformation methods that enable the removal of selectable marker gen...

    Authors: Fang Liu, Pandi Wang, Xiaojuan Xiong, Ping Fu, Hongfei Gao, Xinhua Ding and Gang Wu

    Citation: Plant Methods 2020 16:81

    Content type: Research

    Published on:

  43. Obtaining high-quality RNA for gene expression analyses from different seed tissues is challenging due to the presence of various contaminants, such as polyphenols, polysaccharides and lipids which interfere w...

    Authors: Laura Siles, Peter Eastmond and Smita Kurup

    Citation: Plant Methods 2020 16:80

    Content type: Methodology

    Published on:

  44. The number of kernels per ear is one of the major agronomic yield indicators for maize. Manual assessment of kernel traits can be time consuming and laborious. Moreover, manually acquired data can be influence...

    Authors: Di Wu, Zhen Cai, Jiwan Han and Huawei Qin

    Citation: Plant Methods 2020 16:79

    Content type: Methodology

    Published on:

  45. A fast, reliable and non-destructive method is needed to qualify the extractives content (EC) in heartwood of T. sinensis cores in the breeding program for studying the genetic effect on EC. However, the influenc...

    Authors: Yanjie Li, Xin Dong, Yang Sun, Jun Liu and Jingmin Jiang

    Citation: Plant Methods 2020 16:77

    Content type: Research

    Published on:

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