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  1. The genetic basis of colour development in red-flesh apples (Malus domestica Borkh) has been widely characterised; however, current models do not explain the observed variations in red pigmentation intensity and ...

    Authors: Pierre Bouillon, Anne-Laure Fanciullino, Etienne Belin, Dimitri Bréard, Séverine Boisard, Béatrice Bonnet, Sylvain Hanteville, Frédéric Bernard and Jean-Marc Celton
    Citation: Plant Methods 2024 20:71
  2. Phytophthora sojae, a soil-borne oomycete pathogen, has been a yield limiting factor for more than 60 years on soybean. The resurgence of P. sojae (Phytophthora sojae) is primarily ascribed to the durable oospore...

    Authors: Xiaomeng Chu, Ziyi Yin, Pengjie Yue, Xinyu Wang, Yue Yang, Jiayi Sun, Ziying Kong, Jian Ren, Xiaohan Liu, Chongchong Lu, Haipeng Zhao, Yang Li and Xinhua Ding
    Citation: Plant Methods 2024 20:70
  3. Characterisation of the structure and water status of leaf tissues is essential to the understanding of leaf hydraulic functioning under optimal and stressed conditions. Magnetic Resonance Imaging is unique in...

    Authors: Pierre-Nicolas Boulc’h, Guylaine Collewet, Baptiste Guillon, Stéphane Quellec, Laurent Leport and Maja Musse
    Citation: Plant Methods 2024 20:69
  4. With the accumulating omics data, an efficient and time-saving transient assay to express target genes is desired. Mesophyll protoplasts, maintaining most stress-physiological responses and cellular activities...

    Authors: Shanshan Lei, Yaolong Zhu, Weiyu Jia, Jing Zhang, Yingjun Chi and Bin Xu
    Citation: Plant Methods 2024 20:67
  5. Citrus canker is a significant bacterial disease caused by Xanthomonas citri subsp. citri (Xcc) that severely impedes the healthy development of the citrus industry. Especially when citrus fruit is infected by Xc...

    Authors: Lian Liu, Xin Liu, Lingyi Liu, Tao Zhu, Rongchun Ye, Hao Chen, Linglei Zhou, Guang Wu, Limei Tan, Jian Han, Ronghua Li, Xianfeng Ma and Ziniu Deng
    Citation: Plant Methods 2024 20:65
  6. Cassava leaf samples degrade quickly during storage and transportation from distant areas. Proper sampling and efficient, low-cost storage methods are critical for obtaining sufficient quality DNA and RNA for ...

    Authors: Deogratius Mark, Fred Tairo, Joseph Ndunguru, Elisiana Kweka, Maliha Saggaf, Hilda Bachwenkizi, Evangelista Chiunga, James Leonard Lusana, Geofrey Sikazwe and Reuben Maghembe
    Citation: Plant Methods 2024 20:64
  7. The detection of internal defects in seeds via non-destructive imaging techniques is a topic of high interest to optimize the quality of seed lots. In this context, X-ray imaging is especially suited. Recent s...

    Authors: Sherif Hamdy, Aurélie Charrier, Laurence Le Corre, Pejman Rasti and David Rousseau
    Citation: Plant Methods 2024 20:63
  8. High-quality RNA extraction from woody plants is difficult because of the presence of polysaccharides and polyphenolics that bind or co-precipitate with the RNA. The CTAB (cetyl trimethylammonium bromide) base...

    Authors: Tibor Kiss, Zoltán Karácsony, Adrienn Gomba-Tóth, Kriszta Lilla Szabadi, Zsolt Spitzmüller, Júlia Hegyi-Kaló, Thomas Cels, Margot Otto, Richárd Golen, Ádám István Hegyi, József Geml and Kálmán Zoltán Váczy
    Citation: Plant Methods 2024 20:62
  9. Despite major efforts over the last decades, the rising demands of the growing global population makes it of paramount importance to increase crop yields and reduce losses caused by plant pathogens. One way to...

    Authors: Mackenzie Eli William Loranger, Winfield Yim, Vittorio Accomazzi, Nadia Morales-Lizcano, Wolfgang Moeder and Keiko Yoshioka
    Citation: Plant Methods 2024 20:60
  10. Artemisia campestris L. (AC) leaves are widely recognized for their importance in traditional medicine. Despite the considerable amount of research conducted on this plant overworld, the chemical composition and ...

    Authors: Inès Limam, Ridha Ghali, Mohamed Abdelkarim, Anis Ouni, Manel Araoud, Mouaadh Abdelkarim, Abderrazek Hedhili and Fatma Ben-Aissa Fennira
    Citation: Plant Methods 2024 20:59
  11. Traditional method of wood species identification involves the use of hand lens by wood anatomists, which is a time-consuming method that usually identifies only at the genetic level. Computer vision method ca...

    Authors: Chang Zheng, Shoujia Liu, Jiajun Wang, Yang Lu, Lingyu Ma, Lichao Jiao, Juan Guo, Yafang Yin and Tuo He
    Citation: Plant Methods 2024 20:56
  12. Improper management of agricultural and industrial cotton wastes causes environmental pollution and worsens the climate change challenge. Green recycling of cotton could contribute to a circular economy. One o...

    Authors: Mona A. Mohammed, Nagat M. Amer, Heba M. I. Abdallah and Mai S. Saleh
    Citation: Plant Methods 2024 20:54
  13. seeds that are recalcitrant to desiccation and freezing temperatures cannot be stored in gene banks under conventional conditions. However, the germplasm of some recalcitrant seeded species can be stored in li...

    Authors: Urszula Wasileńczyk, Mikołaj Krzysztof Wawrzyniak, João Paulo Rodrigues Martins, Paulina Kosek and Paweł Chmielarz
    Citation: Plant Methods 2024 20:53
  14. Salsola laricifolia is a typical C3–C4 typical desert plant, belonging to the family Amaranthaceae. An efficient single-cell system is crucial to study the gene function of this plant. In this study, we optimized...

    Authors: Hao Guo, Yuxin Xi, Kuerban Guzailinuer and Zhibin Wen
    Citation: Plant Methods 2024 20:52
  15. Stemphylium blight incited by Stemphylium botryosum poses a significant threat to lentil crops worldwide, inducing severe defoliation and causing substantial yield losses in susceptible varieties under favorable ...

    Authors: Mario González, Eleonora Barilli, Nicolas Rispail and Diego Rubiales
    Citation: Plant Methods 2024 20:51
  16. The root of a plant is a fundamental organ for the multisensory perception of the environment. Investigating root growth dynamics as a mean of their interaction with the environment is of key importance for im...

    Authors: Valentina Simonetti, Laura Ravazzolo, Benedetto Ruperti, Silvia Quaggiotti and Umberto Castiello
    Citation: Plant Methods 2024 20:50
  17. Leaf water content (LWC) significantly affects rice growth and development. Real-time monitoring of rice leaf water status is essential to obtain high yield and water use efficiency of rice plants with precise...

    Authors: Xuenan Zhang, Haocong Xu, Yehong She, Chao Hu, Tiezhong Zhu, Lele Wang, Liquan Wu, Cuicui You, Jian Ke, Qiangqiang Zhang and Haibing He
    Citation: Plant Methods 2024 20:48
  18. Cotton accounts for 80% of the global natural fibre production. Its leaf hairiness affects insect resistance, fibre yield, and economic value. However, this phenotype is still qualitatively assessed by visuall...

    Authors: Moshiur Farazi, Warren C. Conaty, Lucy Egan, Susan P. J. Thompson, Iain W. Wilson, Shiming Liu, Warwick N. Stiller, Lars Petersson and Vivien Rolland
    Citation: Plant Methods 2024 20:46
  19. For ten years, CRISPR/cas9 system has become a very useful tool for obtaining site-specific mutations on targeted genes in many plant organisms. This technology opens up a wide range of possibilities for impro...

    Authors: Jérémy Villette, Fatma Lecourieux, Eliot Bastiancig, Marie-Claire Héloir and Benoit Poinssot
    Citation: Plant Methods 2024 20:45
  20. Plant scientists have largely relied on pathogen growth assays and/or transcript analysis of stress-responsive genes for quantification of disease severity and susceptibility. These methods are destructive to ...

    Authors: Jae Hoon Lee, Unseok Lee, Ji Hye Yoo, Taek Sung Lee, Je Hyeong Jung and Hyoung Seok Kim
    Citation: Plant Methods 2024 20:44
  21. Dendrobium officinale is a medicinal plant with high commercial value. The Dendrobium officinale market in Yunnan is affected by the standardization of medicinal material quality control and the increase in marke...

    Authors: Peiyuan Li, Tao shen, Li Li and Yuanzhong Wang
    Citation: Plant Methods 2024 20:43
  22. Genomic selection (GS) has become an increasingly popular tool in plant breeding programs, propelled by declining genotyping costs, an increase in computational power, and rediscovery of the best linear unbias...

    Authors: Javier Fernández-González, Bertrand Haquin, Eliette Combes, Karine Bernard, Alix Allard and Julio Isidro y Sánchez
    Citation: Plant Methods 2024 20:42

    The Correction to this article has been published in Plant Methods 2024 20:66

  23. Gaseous phytohormone ethylene levels are directly influenced by the production of its immediate non-volatile precursor 1-aminocyclopropane-1-carboxylic acid (ACC). Owing to the strongly acidic character of the...

    Authors: Michal Karady, Pavel Hladík, Kateřina Cermanová, Petra Jiroutová, Ioanna Antoniadi, Rubén Casanova-Sáez, Karin Ljung and Ondřej Novák
    Citation: Plant Methods 2024 20:41
  24. Quantifying gene expression is a critical aspect of applied genomics research across all organisms, and real-time PCR has emerged as a powerful tool for this purpose. However, selecting appropriate internal co...

    Authors: Amin Ebrahimi, Shahrokh Gharanjik, Elham Azadvari and Sajad Rashidi-Monfared
    Citation: Plant Methods 2024 20:40
  25. Climate instability directly affects agro-environments. Water scarcity, high air temperature, and changes in soil biota are some factors caused by environmental changes. Verified and precise phenotypic traits ...

    Authors: Duvan Pineda-Castro, Harold Diaz, Jonatan Soto and Milan Oldřich Urban
    Citation: Plant Methods 2024 20:39
  26. The extraction of thylakoids is an essential step in studying the structure of photosynthetic complexes and several other aspects of the photosynthetic process in plants. Conventional protocols have been devel...

    Authors: Quentin Charras, Pascal Rey, Dorian Guillemain, Fabian Dourguin, Hugo Laganier, Sacha Peschoux, Roland Molinié, Marwa Ismaël, Stefano Caffarri, Catherine Rayon and Colette Jungas
    Citation: Plant Methods 2024 20:38
  27. Interspecific hybridisation is a powerful tool for increasing genetic diversity in plant breeding programmes. Hexaploid wheat (Triticum aestivum, 2n = 42) × barley (Hordeum vulgare, 2n = 14) intergeneric hybrids ...

    Authors: Mohammad Ali, Dávid Polgári, Adél Sepsi, Levente Kontra, Ágnes Dalmadi, Zoltán Havelda, László Sági and András Kis
    Citation: Plant Methods 2024 20:37

    The Correction to this article has been published in Plant Methods 2024 20:55

  28. Crop genetic engineering for better root systems can offer practical solutions for food security and carbon sequestration; however, soil layers prevent the direct visualization of plant roots, thus posing a ch...

    Authors: Mika Tei, Fumiyuki Soma, Ettore Barbieri, Yusaku Uga and Yosuke Kawahito
    Citation: Plant Methods 2024 20:36
  29. The development and physiology of plants are influenced by light intensity and its changes. Despite the significance of this phenomenon, there is a lack of understanding regarding the processes light regulates...

    Authors: Ludovico Caracciolo, John Philippi, Tom P. J. M. Theeuwen, Herbert van Amerongen and Jeremy Harbinson
    Citation: Plant Methods 2024 20:35
  30. More and more studies show that miRNA plays a crucial role in plants' response to different abiotic stresses. However, traditional experimental methods are often expensive and inefficient, so it is important t...

    Authors: Liming Chang, Xiu Jin, Yuan Rao and Xiaodan Zhang
    Citation: Plant Methods 2024 20:33
  31. Plant physiology and structure are constantly changing according to internal and external factors. The study of plant water dynamics can give information on these changes, as they are linked to numerous plant ...

    Authors: Shannan Blystone, Magali Nuixe, Amidou Sissou Traoré, Hervé Cochard, Catherine Picon-Cochard and Guilhem Pagés
    Citation: Plant Methods 2024 20:31
  32. The use of high-performant varieties could help to improve the production of food legumes and thus meet the demand of the growing world population. However, long periods needed to develop new varieties through...

    Authors: Mohammed Mitache, Aziz Baidani, Bouchaib Bencharki and Omar Idrissi
    Citation: Plant Methods 2024 20:30
  33. Hairy roots constitute a valuable tissue culture system for species that are difficult to propagate through conventional seed-based methods. Moreover, the generation of transgenic plants derived from hairy roo...

    Authors: Veronika Jedličková, Marie Štefková, Terezie Mandáková, Juan Francisco Sánchez López, Marek Sedláček, Martin A. Lysak and Hélène S. Robert
    Citation: Plant Methods 2024 20:29
  34. The challenge of pigeonpea breeding lies in its photosensitivity and seasonal specificity. This poses a problem to the breeder, as it restricts to single generation advancement in a year. Currently, the cross ...

    Authors: Prakash I. Gangashetty, Shruthi H. Belliappa, Naresh Bomma, Vinutha Kanuganahalli, Sobhan Babu Sajja, Sunita Choudhary, Ramanagouda Gaviyappanavar, Deekshitha Bomireddy, V. Anil Kumar, Jwala Pranati, Mamta Sharma and Manish K. Pandey
    Citation: Plant Methods 2024 20:27
  35. Virus-induced gene silencing (VIGS) is widely used in plant functional genomics. However, the efficiency of VIGS in young plantlets varies across plant species. Additionally, VIGS is not optimized for many pla...

    Authors: Yongliang Liu, Ruiqing Lyu, Joshua J. Singleton, Barunava Patra, Sitakanta Pattanaik and Ling Yuan
    Citation: Plant Methods 2024 20:26
  36. Mastering the spatial distribution and planting area of paddy can provide a scientific basis for monitoring rice production, and planning grain production layout. Previous remote sensing studies on paddy conce...

    Authors: Lihua Wang, Hao Ma, Yanghua Gao, Shengbo Chen, Songling Yang, Peng Lu, Li Fan and Yumiao Wang
    Citation: Plant Methods 2024 20:25
  37. Camellia oleifera, an essential woody oil tree in China, propagates through grafting. However, in production, it has been found that the interaction between rootstocks and scions may affect fruit characteristics....

    Authors: Fan Yang, Yuhuan Zhou, Jiayi Du, Kailiang Wang, Leyan Lv and Wei Long
    Citation: Plant Methods 2024 20:23

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