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  1. Content type: Research

    Precise control of gene expression is essential to synchronize plant development with the environment. In perennial plants, transcriptional regulation remains poorly understood, mainly due to the long time req...

    Authors: José M. Ramos-Sánchez, Paolo M. Triozzi, Alicia Moreno-Cortés, Daniel Conde, Mariano Perales and Isabel Allona

    Citation: Plant Methods 2017 13:50

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  2. Content type: Methodology

    Within the Brassicaceae, six species from the genus Brassica are widely cultivated throughout the world as oilseed, condiment, fodder or vegetable crops. The genetic relationships among the six Brassica species a...

    Authors: Joshua C. O. Koh, Denise M. Barbulescu, Sally Norton, Bob Redden, Phil A. Salisbury, Sukhjiwan Kaur, Noel Cogan and Anthony T. Slater

    Citation: Plant Methods 2017 13:49

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  3. Content type: Methodology

    In order to better understand and improve the mode of action of agrochemicals, it is useful to be able to visualize their uptake and distribution in vivo, non-invasively and, ideally, in the field. Here we exp...

    Authors: Elizabeth Noble, Sunil Kumar, Frederik G. Görlitz, Chris Stain, Chris Dunsby and Paul M. W. French

    Citation: Plant Methods 2017 13:48

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  4. Content type: Methodology

    Hyperspectral imaging is an emerging means of assessing plant vitality, stress parameters, nutrition status, and diseases. Extraction of target values from the high-dimensional datasets either relies on pixel-...

    Authors: Uwe Knauer, Andrea Matros, Tijana Petrovic, Timothy Zanker, Eileen S. Scott and Udo Seiffert

    Citation: Plant Methods 2017 13:47

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  5. Content type: Methodology

    Perennial ryegrass (Lolium perenne L.) is an important temperate grass used for turf and forage purposes. With the increasing accumulation of genomic and transcriptomic data of perennial ryegrass, an efficient pr...

    Authors: Guohui Yu, Qiang Cheng, Zheni Xie, Bin Xu, Bingru Huang and Bingyu Zhao

    Citation: Plant Methods 2017 13:46

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  6. Content type: Methodology

    Chlorophyll fluorescence (CF) is a key indicator to study plant physiology or photosynthesis efficiency. Conventionally, CF is characterized by fluorometers, which only allows ensemble measurement through wide...

    Authors: Yi-Chin Tseng and Shi-Wei Chu

    Citation: Plant Methods 2017 13:43

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  7. Content type: Methodology

    Binding of transcription factors to their target sequences is a primary step in the regulation of gene expression and largely determines gene regulatory networks. Chromatin immunoprecipitation (ChIP) is an ind...

    Authors: Jeong Hwan Lee, Suhyun Jin, Sun Young Kim, Wanhui Kim and Ji Hoon Ahn

    Citation: Plant Methods 2017 13:42

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  8. Content type: Research

    The main aim of this study was to improve fungal resistance in bread wheat via transgenesis. Transgenic wheat plants harboring barley chitinase (chi26) gene, driven by maize ubi promoter, were obtained using biol...

    Authors: Hala F. Eissa, Sameh E. Hassanien, Ahmed M. Ramadan, Moustafa M. El-Shamy, Osama M. Saleh, Ahmed M. Shokry, Mohamed Abdelsattar, Yasser B. Morsy, Maher A. El-Maghraby, Hussien F. Alameldin, Sabah M. Hassan, Gamal H. Osman, Hesham T. Mahfouz, Gharib A. Gad El-Karim, Magdy A. Madkour and Ahmed Bahieldin

    Citation: Plant Methods 2017 13:41

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  9. Content type: Methodology

    Positron imaging can be used to non-destructively visualize the dynamics of a positron-emitting radionuclide in vivo, and is therefore a tool for understanding the mechanisms of nutrient transport in intact pl...

    Authors: Nobuo Suzui, Yong-Gen Yin, Satomi Ishii, Hitoshi Sekimoto and Naoki Kawachi

    Citation: Plant Methods 2017 13:40

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  10. Content type: Methodology

    Detection of plant diseases in a fast and simple way is crucial for timely disease control. Conventionally, plant diseases are accurately identified by DNA, RNA or serology based methods which are time consumi...

    Authors: Chu Zhang, Xuping Feng, Jian Wang, Fei Liu, Yong He and Weijun Zhou

    Citation: Plant Methods 2017 13:39

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  11. Content type: Methodology

    Plant density and its non-uniformity drive the competition among plants as well as with weeds. They need thus to be estimated with small uncertainties accuracy. An optimal sampling method is proposed to estima...

    Authors: Shouyang Liu, Fred Baret, Denis Allard, Xiuliang Jin, Bruno Andrieu, Philippe Burger, Matthieu Hemmerlé and Alexis Comar

    Citation: Plant Methods 2017 13:38

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  12. Content type: Methodology

    The unicellular green alga, Chlamydomonas reinhardtii, is a classic model for studying flagella and biofuel. However, precise gene editing, such as Clustered Regularly Interspaced Short Palindromic Repeats (CRISP...

    Authors: Xi Cheng, Gai Liu, Wenting Ke, Lijuan Zhao, Bo Lv, Xiaocui Ma, Nannan Xu, Xiaoling Xia, Xuan Deng, Chunlei Zheng and Kaiyao Huang

    Citation: Plant Methods 2017 13:36

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  13. Content type: Methodology

    The Photochemical Reflectance Index (PRI) calculated from narrow-band spectral reflectance data is a vegetation index which is increasingly used as an indicator of photosynthetic activity. The leaf-level link ...

    Authors: Matti Mõttus, Rocío Hernández-Clemente, Viljami Perheentupa and Vincent Markiet

    Citation: Plant Methods 2017 13:35

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  14. Content type: Methodology

    Reduction in rice yield caused by high temperature-induced spikelet sterility has been a serious concern in rice production. To date, several screening methods have been used, although their reproducibility i...

    Authors: Makoto Hakata, Hiroshi Wada, Chisato Masumoto-Kubo, Ryo Tanaka, Hiroyuki Sato and Satoshi Morita

    Citation: Plant Methods 2017 13:34

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  15. Content type: Methodology

    Archegoniates (bryophytes, ferns and gymnosperms), such as the moss Physcomitrella patens, possess freely motile sperm cells (spermatozoids) which reach the egg cell via surface water. Although these motile flage...

    Authors: Nelly A. Horst and Ralf Reski

    Citation: Plant Methods 2017 13:33

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  16. Content type: Methodology

    PTI and ETI are the two major defence mechanisms in plants. ETI is triggered by the detection of pathogen effectors, or their activity, in the plant cell and most of the time involves internal receptors known ...

    Authors: Cyril Brendolise, Mirco Montefiori, Romain Dinis, Nemo Peeters, Roy D. Storey and Erik H. Rikkerink

    Citation: Plant Methods 2017 13:32

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  17. Content type: Review

    Chloroplasts play a great role for sustained wellbeing of life on the planet. They have the power and raw materials that can be used as sophisticated biological factories. They are rich in energy as they have ...

    Authors: Muhamed Adem, Dereje Beyene and Tileye Feyissa

    Citation: Plant Methods 2017 13:30

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  18. Content type: Methodology

    The ability to target and manipulate protein-based cellular processes would accelerate plant research; yet, the technology to specifically and selectively target plant-expressed proteins is still in its infanc...

    Authors: André C. Velásquez, Kinya Nomura, Max D. Cooper, Brantley R. Herrin and Sheng Yang He

    Citation: Plant Methods 2017 13:29

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  19. Content type: Methodology

    Sugarcane (Saccharum spp.) is the main raw material for sugar and ethanol production. Among the abiotic stress, drought is the main one that negatively impact sugarcane yield. Although gene expression analysis th...

    Authors: Larissa Mara de Andrade, Michael dos Santos Brito, Rafael Fávero Peixoto Junior, Paulo Eduardo Ribeiro Marchiori, Paula Macedo Nóbile, Alexandre Palma Boer Martins, Rafael Vasconcelos Ribeiro and Silvana Creste

    Citation: Plant Methods 2017 13:28

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  20. Content type: Methodology

    Endoreduplication, the process of DNA replication in the absence of cell division, is associated with specialized cellular function and increased cell size. Genes controlling endoreduplication in tomato fruit ...

    Authors: F. Parker E. Laimbeer, Sarah H. Holt, Melissa Makris, Michael Alan Hardigan, C. Robin Buell and Richard E. Veilleux

    Citation: Plant Methods 2017 13:27

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  21. Content type: Methodology

    Chlorophyll content decreases in plants under stress conditions, therefore it is used commonly as an indicator of plant health. Arabidopsis thaliana offers a convenient and fast way to test physiological phenotyp...

    Authors: Ying Liang, Daisuke Urano, Kang-Ling Liao, Tyson L. Hedrick, Yajun Gao and Alan M. Jones

    Citation: Plant Methods 2017 13:26

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  22. Content type: Methodology

    Peanut (Arachis hypogaea) production is largely affected by a variety of abiotic and biotic stresses, including the root-knot nematode (RKN) Meloidogyne arenaria that causes yield losses worldwide. Transcriptome ...

    Authors: Larissa Arrais Guimaraes, Bruna Medeiros Pereira, Ana Claudia Guerra Araujo, Patricia Messenberg Guimaraes and Ana Cristina Miranda Brasileiro

    Citation: Plant Methods 2017 13:25

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  23. Content type: Methodology

    Phenotyping is a critical component of plant research. Accurate and precise trait collection, when integrated with genetic tools, can greatly accelerate the rate of genetic gain in crop improvement. However, e...

    Authors: Hsiang Sing Naik, Jiaoping Zhang, Alec Lofquist, Teshale Assefa, Soumik Sarkar, David Ackerman, Arti Singh, Asheesh K. Singh and Baskar Ganapathysubramanian

    Citation: Plant Methods 2017 13:23

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  24. Content type: Methodology

    Random insertional mutagenesis of Chlamydomonas reinhardtii using drug resistance cassettes has contributed to the generation of tens of thousands of transformants in dozens of labs around the world. In many inst...

    Authors: Steve V. Pollock, Bratati Mukherjee, Joanna Bajsa-Hirschel, Marylou C. Machingura, Ananya Mukherjee, Arthur R. Grossman and James V. Moroney

    Citation: Plant Methods 2017 13:22

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  25. Content type: Methodology

    The maize male inflorescence (tassel) produces pollen necessary for reproduction and commercial grain production of maize. The size of the tassel has been linked to factors affecting grain yield, so understand...

    Authors: Joseph L. Gage, Nathan D. Miller, Edgar P. Spalding, Shawn M. Kaeppler and Natalia de Leon

    Citation: Plant Methods 2017 13:21

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  26. Content type: Research

    The liverwort Marchantia polymorpha occupies a crucial position in land plant evolution and provides the opportunity to investigate adaptations to a terrestrial plant life style. Marchantia reverse genetic analys...

    Authors: Sarah Kopischke, Esther Schüßler, Felix Althoff and Sabine Zachgo

    Citation: Plant Methods 2017 13:20

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  27. Content type: Research

    Cytoskeleton can be observed in live plant cells in situ with high spatial and temporal resolution using a combination of specific fluorescent protein tag expression and advanced microscopy methods such as spi...

    Authors: Fatima Cvrčková and Denisa Oulehlová

    Citation: Plant Methods 2017 13:19

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  28. Content type: Methodology

    Smarthouses capable of non-destructive, high-throughput plant phenotyping collect large amounts of data that can be used to understand plant growth and productivity in extreme environments. The challenge is to...

    Authors: Rui Meng, Stephanie Saade, Sebastian Kurtek, Bettina Berger, Chris Brien, Klaus Pillen, Mark Tester and Ying Sun

    Citation: Plant Methods 2017 13:18

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  29. Content type: Methodology

    Glucosinolates, anionic sulfur rich secondary metabolites, have been extensively studied because of their occurrence in the agriculturally important brassicaceae and their impact on human and animal health. Th...

    Authors: T. Doheny-Adams, K. Redeker, V. Kittipol, I. Bancroft and S. E. Hartley

    Citation: Plant Methods 2017 13:17

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  30. Content type: Methodology

    Mitochondrial respiration in the dark (R dark) is a critical plant physiological process, and hence a reliable, efficient and high-throughput method of measuring variation in rates of R

    Authors: Andrew P. Scafaro, A. Clarissa A. Negrini, Brendan O’Leary, F. Azzahra Ahmad Rashid, Lucy Hayes, Yuzhen Fan, You Zhang, Vincent Chochois, Murray R. Badger, A. Harvey Millar and Owen K. Atkin

    Citation: Plant Methods 2017 13:16

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  31. Content type: Methodology

    Robust segmentation of canopy cover (CC) from large amounts of images taken under different illumination/light conditions in the field is essential for high throughput field phenotyping (HTFP). We attempted to...

    Authors: Kang Yu, Norbert Kirchgessner, Christoph Grieder, Achim Walter and Andreas Hund

    Citation: Plant Methods 2017 13:15

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  32. Content type: Methodology

    Biogenic volatile organic compounds (BVOC) emitted by plants play an important role for ecological and physiological processes, for example as response to stressors. These emitted compounds are involved in che...

    Authors: Marvin Lüpke, Rainer Steinbrecher, Michael Leuchner and Annette Menzel

    Citation: Plant Methods 2017 13:14

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    The Correction to this article has been published in Plant Methods 2017 13:100

  33. Content type: Methodology

    Growth is an important parameter to consider when studying the impact of treatments or mutations on plant physiology. Leaf area and growth rates can be estimated efficiently from images of plants, but the expe...

    Authors: Filipa Tomé, Karel Jansseune, Bernadette Saey, Jack Grundy, Korneel Vandenbroucke, Matthew A. Hannah and Henning Redestig

    Citation: Plant Methods 2017 13:13

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  34. Content type: Software

    Computer-based phenotyping of plants has risen in importance in recent years. Whilst much software has been written to aid phenotyping using image analysis, to date the vast majority has been only semi-automat...

    Authors: Michael P. Pound, Susan Fozard, Mercedes Torres Torres, Brian G. Forde and Andrew P. French

    Citation: Plant Methods 2017 13:12

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  35. Content type: Methodology

    Belowground processes play an essential role in ecosystem nutrient cycling and the global carbon budget cycle. Quantifying fine root growth is crucial to the understanding of ecosystem structure and function a...

    Authors: Awaz Mohamed, Yogan Monnier, Zhun Mao, Guillaume Lobet, Jean-Luc Maeght, Merlin Ramel and Alexia Stokes

    Citation: Plant Methods 2017 13:11

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  36. Content type: Methodology

    Chemical genetics provides a powerful alternative to conventional genetics for understanding gene function. However, its application to plants has been limited by the lack of a technology that allows detailed ...

    Authors: Thomas Burrell, Susan Fozard, Geoff H. Holroyd, Andrew P. French, Michael P. Pound, Christopher J. Bigley, C. James Taylor and Brian G. Forde

    Citation: Plant Methods 2017 13:10

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  37. Content type: Methodology

    Accurate floral staging is required to aid research into pollen and flower development, in particular male development. Pollen development is highly sensitive to stress and is critical for crop yields. Researc...

    Authors: Saoirse R. Tracy, José Fernández Gómez, Craig J. Sturrock, Zoe A. Wilson and Alison C. Ferguson

    Citation: Plant Methods 2017 13:9

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  38. Content type: Research

    The fitness of the rape leaf is closely related to its biomass and photosynthesis. The study of leaf traits is significant for improving rape leaf production and optimizing crop management. Canopy structure an...

    Authors: Xiong Xiong, Lejun Yu, Wanneng Yang, Meng Liu, Ni Jiang, Di Wu, Guoxing Chen, Lizhong Xiong, Kede Liu and Qian Liu

    Citation: Plant Methods 2017 13:7

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  39. Content type: Research

    Due to the complex process of designing and manufacturing new plant tissue culture vessels through conventional means there have been limited efforts to innovate improved designs. Further, development and avai...

    Authors: Mukund R. Shukla, Amritpal S. Singh, Kevin Piunno, Praveen K. Saxena and A. Maxwell P. Jones

    Citation: Plant Methods 2017 13:6

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  40. Content type: Methodology

    Biological materials have a complex, hierarchical structure, with vital structural features present at all size scales, from the nanoscale to the macroscale. A method that can connect information at multiple l...

    Authors: Patrik Ahvenainen, Patrick G. Dixon, Aki Kallonen, Heikki Suhonen, Lorna J. Gibson and Kirsi Svedström

    Citation: Plant Methods 2017 13:5

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  41. Content type: Research

    Modern agriculture uses hyperspectral cameras to obtain hundreds of reflectance data measured at discrete narrow bands to cover the whole visible light spectrum and part of the infrared and ultraviolet light s...

    Authors: Osval A. Montesinos-López, Abelardo Montesinos-López, José Crossa, Gustavo de los Campos, Gregorio Alvarado, Mondal Suchismita, Jessica Rutkoski, Lorena González-Pérez and Juan Burgueño

    Citation: Plant Methods 2017 13:4

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  42. Content type: Methodology

    Virus-induced gene silencing (VIGS) has become a powerful tool for post-genomic technology in plant species. This is important, especially in select plants, such as the pepper plant, that are recalcitrant to Agro...

    Authors: Jihyun Kim, Minkyu Park, Eun Soo Jeong, Je Min Lee and Doil Choi

    Citation: Plant Methods 2017 13:3

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  43. Content type: Methodology

    The analysis of circadian leaf movement rhythms is a simple yet effective method to study effects of treatments or gene mutations on the circadian clock of plants. Currently, leaf movements are analysed using ...

    Authors: Lucas Wagner, Christoph Schmal, Dorothee Staiger and Selahattin Danisman

    Citation: Plant Methods 2017 13:2

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