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  1. Content type: Research

    Floral timing is a carefully regulated process, in which the plant determines the optimal moment to switch from the vegetative to reproductive phase. While there are numerous genes known that control flowering...

    Authors: Martijn Fiers, Jorin Hoogenboom, Alice Brunazzi, Tom Wennekes, Gerco C. Angenent and Richard G. H. Immink

    Citation: Plant Methods 2017 13:78

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  2. Content type: Research

    Changing the phosphorus (P) nutrition leads to changes in plant metabolism. The aim of this study was to investigate how these changes are reflected in the distribution of 33P and the isotopic composition of oxy...

    Authors: Verena Pfahler, Federica Tamburini, Stefano M. Bernasconi and Emmanuel Frossard

    Citation: Plant Methods 2017 13:75

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  3. Content type: Research

    Phenotyping technologies are expected to provide predictive power for a range of applications in plant and crop sciences. Here, we use the disease pressure of Beet Cyst Nematodes (BCN) on sugar beet as an illu...

    Authors: Samuel Joalland, Claudio Screpanti, Frank Liebisch, Hubert Vincent Varella, Alain Gaume and Achim Walter

    Citation: Plant Methods 2017 13:73

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  4. Content type: Methodology article

    Celiac disease (CD) is caused by specific sequences of gluten proteins found in cereals such as bread wheat (Triticum aestivum ssp. aestivum) and spelt (T. aestivum ssp. spelta). Among them, the α-gliadins displa...

    Authors: Benjamin Dubois, Pierre Bertin, Yordan Muhovski, Emmanuelle Escarnot and Dominique Mingeot

    Citation: Plant Methods 2017 13:72

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  5. Content type: Methodology article

    Seed–soil contact is important to ensure successful germination, however, there is a paucity of reported studies that have quantified the microstructure at and around this critical interface, mainly due to the...

    Authors: Sebastian Blunk, Ali Hafeez Malik, Martine I. de Heer, Tobias Ekblad, Jennifer Bussell, Debbie Sparkes, Kenneth Fredlund, Craig J. Sturrock and Sacha J. Mooney

    Citation: Plant Methods 2017 13:71

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  6. Content type: Research

    Boron (B) tolerance has been identified as a key target for field pea improvement. Screening for B tolerance in the field is problematic due to variability in space and time, and robust B molecular markers are...

    Authors: Richard G. Bennett, Federico M. Ribalta, Maria Pazos-Navarro, Antonio Leonforte and Janine S. Croser

    Citation: Plant Methods 2017 13:70

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  8. Content type: Methodology article

    Sedentary endoparasitic cyst nematodes form a feeding structure in plant roots, called a syncytium. Syncytium formation involves extensive transcriptional modifications, which leads to cell modifications such ...

    Authors: Rinu Kooliyottil, Louise-Marie Dandurand, Joseph C. Kuhl, Allan Caplan and Fangming Xiao

    Citation: Plant Methods 2017 13:68

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  9. Content type: Methodology article

    Seed development in the angiosperms requires the production of a female gametophyte (embryo sac) within the ovule. Many aspects of female reproductive development in cereal crops are yet to be described, large...

    Authors: Laura G. Wilkinson and Matthew R. Tucker

    Citation: Plant Methods 2017 13:67

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  10. Content type: Methodology

    Common cutworm (CCW; Spodoptera litura Fabricius) is a major leaf-feeding pest of soybean in Asia. The previous methods of measuring antixenosis against CCW using adult plant under field or net-room conditions we...

    Authors: Guangnan Xing, Kai Liu and Junyi Gai

    Citation: Plant Methods 2017 13:66

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  11. Content type: Research

    Understanding root traits is a necessary research front for selection of favorable genotypes or cultivation practices. Root and tuber crops having most of their economic potential stored below ground are favor...

    Authors: Alfredo Delgado, Dirk B. Hays, Richard K. Bruton, Hernán Ceballos, Alexandre Novo, Enrico Boi and Michael Gomez Selvaraj

    Citation: Plant Methods 2017 13:65

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  12. Content type: Research

    Plant glutathione S-transferases (GSTs, EC 2.5.1.18) are multifunctional enzymes involved in heavy metal cellular detoxification by conjugating the tripeptide (g-Glu-Cys-Gly) glutathione to heavy metals. Previous...

    Authors: Zhenzhen Cao, Renxiang Mou, Zhaoyun Cao, Xiaoyan Lin, Youning Ma, Zhiwei Zhu and Mingxue Chen

    Citation: Plant Methods 2017 13:64

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  13. Content type: Methodology article

    Condensed tannins (CT) are the most abundant secondary metabolite of land plants and can vary in abundance and structure according to tissue type, species, genotype, age, and environmental conditions. Recent i...

    Authors: Philip-Edouard Shay, J. A. Trofymow and C. Peter Constabel

    Citation: Plant Methods 2017 13:63

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  14. Content type: Methodology article

    Modern agriculture uses hyperspectral cameras that provide hundreds of reflectance data at discrete narrow bands in many environments. These bands often cover the whole visible light spectrum and part of the i...

    Authors: Abelardo Montesinos-López, Osval A. Montesinos-López, Jaime Cuevas, Walter A. Mata-López, Juan Burgueño, Sushismita Mondal, Julio Huerta, Ravi Singh, Enrique Autrique, Lorena González-Pérez and José Crossa

    Citation: Plant Methods 2017 13:62

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  15. Content type: Methodology article

    Photosynthesis can be roughly separated into biochemical and photochemical processes. Both are affected by drought and can be assessed by non-invasive standard methods. Gas exchange, which mainly assesses the ...

    Authors: Carlos Antônio Ferreira de Sousa, Dayane Silva de Paiva, Raphael Augusto das Chagas Noqueli Casari, Nelson Geraldo de Oliveira, Hugo Bruno Correa Molinari, Adilson Kenji Kobayashi, Paulo Cesar Magalhães, Reinaldo Lúcio Gomide and Manoel Teixeira Souza Jr.

    Citation: Plant Methods 2017 13:61

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  17. Content type: Methodology article

    Virus-induced gene silencing (VIGS) has been used in many plant species as an attractive post transcriptional gene silencing (PTGS) method for studying gene function either individually or at large-scale in a ...

    Authors: Bangjun Zhou and Lirong Zeng

    Citation: Plant Methods 2017 13:59

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  19. Content type: Methodology

    There are numerous systems and techniques to measure the growth of plant roots. However, phenotyping large numbers of plant roots for breeding and genetic analyses remains challenging. One major difficulty is ...

    Authors: Lionel X. Dupuy, Gladys Wright, Jacqueline A. Thompson, Anna Taylor, Sebastien Dekeyser, Christopher P. White, William T. B. Thomas, Mark Nightingale, John P. Hammond, Neil S. Graham, Catherine L. Thomas, Martin R. Broadley and Philip J. White

    Citation: Plant Methods 2017 13:57

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  20. Content type: Methodology

    In sorghum, the growth angle of nodal roots is a major component of root system architecture. It strongly influences the spatial distribution of roots of mature plants in the soil profile, which can impact dro...

    Authors: Dinesh C. Joshi, Vijaya Singh, Colleen Hunt, Emma Mace, Erik van Oosterom, Richard Sulman, David Jordan and Graeme Hammer

    Citation: Plant Methods 2017 13:56

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  21. Content type: Methodology article

    Tree pruning is a costly practice with important implications for crop harvest and nutrition, pest and disease control, soil protection and irrigation strategies. Investigations on tree pruning usually involve...

    Authors: F. M. Jiménez-Brenes, F. López-Granados, A. I. de Castro, J. Torres-Sánchez, N. Serrano and J. M. Peña

    Citation: Plant Methods 2017 13:55

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  22. Content type: Methodology

    Automated plant phenotyping has been established as a powerful new tool in studying plant growth, development and response to various types of biotic or abiotic stressors. Respective facilities mainly apply no...

    Authors: Henning Tschiersch, Astrid Junker, Rhonda C. Meyer and Thomas Altmann

    Citation: Plant Methods 2017 13:54

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  23. Content type: Research

    Low field NMR has been used to investigate water status in various plant tissues. In plants grown in controlled conditions, the method was shown to be able to monitor leaf development as it could detect slight...

    Authors: Maja Musse, Laurent Leport, Mireille Cambert, William Debrandt, Clément Sorin, Alain Bouchereau and François Mariette

    Citation: Plant Methods 2017 13:53

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  25. Content type: Research

    Plant water resource management is one of the main future challenges to fight recent climatic changes. The knowledge of the plant water content could be indispensable for water saving strategies. Terahertz spe...

    Authors: Lorenzo Baldacci, Mario Pagano, Luca Masini, Alessandra Toncelli, Giorgio Carelli, Paolo Storchi and Alessandro Tredicucci

    Citation: Plant Methods 2017 13:51

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  26. Content type: Research

    Precise control of gene expression is essential to synchronize plant development with the environment. In perennial plants, transcriptional regulation remains poorly understood, mainly due to the long time req...

    Authors: José M. Ramos-Sánchez, Paolo M. Triozzi, Alicia Moreno-Cortés, Daniel Conde, Mariano Perales and Isabel Allona

    Citation: Plant Methods 2017 13:50

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  27. Content type: Methodology

    Within the Brassicaceae, six species from the genus Brassica are widely cultivated throughout the world as oilseed, condiment, fodder or vegetable crops. The genetic relationships among the six Brassica species a...

    Authors: Joshua C. O. Koh, Denise M. Barbulescu, Sally Norton, Bob Redden, Phil A. Salisbury, Sukhjiwan Kaur, Noel Cogan and Anthony T. Slater

    Citation: Plant Methods 2017 13:49

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  28. Content type: Methodology

    In order to better understand and improve the mode of action of agrochemicals, it is useful to be able to visualize their uptake and distribution in vivo, non-invasively and, ideally, in the field. Here we exp...

    Authors: Elizabeth Noble, Sunil Kumar, Frederik G. Görlitz, Chris Stain, Chris Dunsby and Paul M. W. French

    Citation: Plant Methods 2017 13:48

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  29. Content type: Methodology

    Hyperspectral imaging is an emerging means of assessing plant vitality, stress parameters, nutrition status, and diseases. Extraction of target values from the high-dimensional datasets either relies on pixel-...

    Authors: Uwe Knauer, Andrea Matros, Tijana Petrovic, Timothy Zanker, Eileen S. Scott and Udo Seiffert

    Citation: Plant Methods 2017 13:47

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  30. Content type: Methodology

    Perennial ryegrass (Lolium perenne L.) is an important temperate grass used for turf and forage purposes. With the increasing accumulation of genomic and transcriptomic data of perennial ryegrass, an efficient pr...

    Authors: Guohui Yu, Qiang Cheng, Zheni Xie, Bin Xu, Bingru Huang and Bingyu Zhao

    Citation: Plant Methods 2017 13:46

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  33. Content type: Methodology

    Chlorophyll fluorescence (CF) is a key indicator to study plant physiology or photosynthesis efficiency. Conventionally, CF is characterized by fluorometers, which only allows ensemble measurement through wide...

    Authors: Yi-Chin Tseng and Shi-Wei Chu

    Citation: Plant Methods 2017 13:43

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  34. Content type: Methodology

    Binding of transcription factors to their target sequences is a primary step in the regulation of gene expression and largely determines gene regulatory networks. Chromatin immunoprecipitation (ChIP) is an ind...

    Authors: Jeong Hwan Lee, Suhyun Jin, Sun Young Kim, Wanhui Kim and Ji Hoon Ahn

    Citation: Plant Methods 2017 13:42

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  35. Content type: Research

    The main aim of this study was to improve fungal resistance in bread wheat via transgenesis. Transgenic wheat plants harboring barley chitinase (chi26) gene, driven by maize ubi promoter, were obtained using biol...

    Authors: Hala F. Eissa, Sameh E. Hassanien, Ahmed M. Ramadan, Moustafa M. El-Shamy, Osama M. Saleh, Ahmed M. Shokry, Mohamed Abdelsattar, Yasser B. Morsy, Maher A. El-Maghraby, Hussien F. Alameldin, Sabah M. Hassan, Gamal H. Osman, Hesham T. Mahfouz, Gharib A. Gad El-Karim, Magdy A. Madkour and Ahmed Bahieldin

    Citation: Plant Methods 2017 13:41

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  36. Content type: Methodology

    Positron imaging can be used to non-destructively visualize the dynamics of a positron-emitting radionuclide in vivo, and is therefore a tool for understanding the mechanisms of nutrient transport in intact pl...

    Authors: Nobuo Suzui, Yong-Gen Yin, Satomi Ishii, Hitoshi Sekimoto and Naoki Kawachi

    Citation: Plant Methods 2017 13:40

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  37. Content type: Methodology

    Detection of plant diseases in a fast and simple way is crucial for timely disease control. Conventionally, plant diseases are accurately identified by DNA, RNA or serology based methods which are time consumi...

    Authors: Chu Zhang, Xuping Feng, Jian Wang, Fei Liu, Yong He and Weijun Zhou

    Citation: Plant Methods 2017 13:39

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  38. Content type: Methodology

    Plant density and its non-uniformity drive the competition among plants as well as with weeds. They need thus to be estimated with small uncertainties accuracy. An optimal sampling method is proposed to estima...

    Authors: Shouyang Liu, Fred Baret, Denis Allard, Xiuliang Jin, Bruno Andrieu, Philippe Burger, Matthieu Hemmerlé and Alexis Comar

    Citation: Plant Methods 2017 13:38

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  40. Content type: Methodology

    The unicellular green alga, Chlamydomonas reinhardtii, is a classic model for studying flagella and biofuel. However, precise gene editing, such as Clustered Regularly Interspaced Short Palindromic Repeats (CRISP...

    Authors: Xi Cheng, Gai Liu, Wenting Ke, Lijuan Zhao, Bo Lv, Xiaocui Ma, Nannan Xu, Xiaoling Xia, Xuan Deng, Chunlei Zheng and Kaiyao Huang

    Citation: Plant Methods 2017 13:36

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  41. Content type: Methodology

    The Photochemical Reflectance Index (PRI) calculated from narrow-band spectral reflectance data is a vegetation index which is increasingly used as an indicator of photosynthetic activity. The leaf-level link ...

    Authors: Matti Mõttus, Rocío Hernández-Clemente, Viljami Perheentupa and Vincent Markiet

    Citation: Plant Methods 2017 13:35

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  42. Content type: Methodology

    Reduction in rice yield caused by high temperature-induced spikelet sterility has been a serious concern in rice production. To date, several screening methods have been used, although their reproducibility i...

    Authors: Makoto Hakata, Hiroshi Wada, Chisato Masumoto-Kubo, Ryo Tanaka, Hiroyuki Sato and Satoshi Morita

    Citation: Plant Methods 2017 13:34

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  43. Content type: Methodology

    Archegoniates (bryophytes, ferns and gymnosperms), such as the moss Physcomitrella patens, possess freely motile sperm cells (spermatozoids) which reach the egg cell via surface water. Although these motile flage...

    Authors: Nelly A. Horst and Ralf Reski

    Citation: Plant Methods 2017 13:33

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  44. Content type: Methodology

    PTI and ETI are the two major defence mechanisms in plants. ETI is triggered by the detection of pathogen effectors, or their activity, in the plant cell and most of the time involves internal receptors known ...

    Authors: Cyril Brendolise, Mirco Montefiori, Romain Dinis, Nemo Peeters, Roy D. Storey and Erik H. Rikkerink

    Citation: Plant Methods 2017 13:32

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  46. Content type: Review

    Chloroplasts play a great role for sustained wellbeing of life on the planet. They have the power and raw materials that can be used as sophisticated biological factories. They are rich in energy as they have ...

    Authors: Muhamed Adem, Dereje Beyene and Tileye Feyissa

    Citation: Plant Methods 2017 13:30

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  47. Content type: Methodology

    The ability to target and manipulate protein-based cellular processes would accelerate plant research; yet, the technology to specifically and selectively target plant-expressed proteins is still in its infanc...

    Authors: André C. Velásquez, Kinya Nomura, Max D. Cooper, Brantley R. Herrin and Sheng Yang He

    Citation: Plant Methods 2017 13:29

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  48. Content type: Methodology

    Sugarcane (Saccharum spp.) is the main raw material for sugar and ethanol production. Among the abiotic stress, drought is the main one that negatively impact sugarcane yield. Although gene expression analysis th...

    Authors: Larissa Mara de Andrade, Michael dos Santos Brito, Rafael Fávero Peixoto Junior, Paulo Eduardo Ribeiro Marchiori, Paula Macedo Nóbile, Alexandre Palma Boer Martins, Rafael Vasconcelos Ribeiro and Silvana Creste

    Citation: Plant Methods 2017 13:28

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  49. Content type: Methodology

    Endoreduplication, the process of DNA replication in the absence of cell division, is associated with specialized cellular function and increased cell size. Genes controlling endoreduplication in tomato fruit ...

    Authors: F. Parker E. Laimbeer, Sarah H. Holt, Melissa Makris, Michael Alan Hardigan, C. Robin Buell and Richard E. Veilleux

    Citation: Plant Methods 2017 13:27

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  50. Content type: Methodology

    Chlorophyll content decreases in plants under stress conditions, therefore it is used commonly as an indicator of plant health. Arabidopsis thaliana offers a convenient and fast way to test physiological phenotyp...

    Authors: Ying Liang, Daisuke Urano, Kang-Ling Liao, Tyson L. Hedrick, Yajun Gao and Alan M. Jones

    Citation: Plant Methods 2017 13:26

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Plant Methods

ISSN: 1746-4811