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  1. Content type: Methodology

    The formation of crossovers during meiosis is pivotal for the redistribution of traits among the progeny of sexually reproducing organisms. In plants the molecular mechanisms underlying the formation of crosso...

    Authors: Niels van Tol, Martijn Rolloos, Peter van Loon and Bert J. van der Zaal

    Citation: Plant Methods 2018 14:32

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  2. Content type: Research

    For in vitro culture of plant and animal cells, one of the critical steps is to adjust the initial cell density. A typical example of this is isolated microspore culture, where specific cell densities have bee...

    Authors: Carolina Camacho-Fernández, David Hervás, Alba Rivas-Sendra, Mª Pilar Marín and Jose M. Seguí-Simarro

    Citation: Plant Methods 2018 14:30

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  3. Content type: Research

    Anthocyanins are abundant secondary metabolites responsible for most blue to blue-black, and red to purple colors of various plant organs. In wheat grains, anthocyanins are accumulated in the pericarp and/or a...

    Authors: Stefan Böhmdorfer, Josua Timotheus Oberlerchner, Christina Fuchs, Thomas Rosenau and Heinrich Grausgruber

    Citation: Plant Methods 2018 14:29

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  4. Content type: Methodology

    PCR allelic discrimination technologies have broad applications in the detection of single nucleotide polymorphisms (SNPs) in genetics and genomics. The use of fluorescence-tagged probes is the leading method ...

    Authors: Chiara Broccanello, Claudia Chiodi, Andrew Funk, J. Mitchell McGrath, Lee Panella and Piergiorgio Stevanato

    Citation: Plant Methods 2018 14:28

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  5. Content type: Methodology

    Accurate and automated phenotyping of leaf images is necessary for high throughput studies of leaf form like genome-wide association analysis and other forms of quantitative trait locus mapping. Dissected leav...

    Authors: Henrik Failmezger, Janne Lempe, Nasim Khadem, Maria Cartolano, Miltos Tsiantis and Achim Tresch

    Citation: Plant Methods 2018 14:27

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  6. Content type: Research

    Guard cell protoplasts (GCPs) isolated from various plants have proven to be especially useful for studies of signal transduction pathways and plant development. But it is not easy to isolate highly purified p...

    Authors: Xuehui Yao, Wenchao Zhao, Rui Yang, Jianli Wang, Fukuan Zhao and Shaohui Wang

    Citation: Plant Methods 2018 14:26

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  7. Content type: Methodology

    The current state-of-the-art for field wood identification to combat illegal logging relies on experienced practitioners using hand lenses, specialized identification keys, atlases of woods, and field manuals....

    Authors: Prabu Ravindran, Adriana Costa, Richard Soares and Alex C. Wiedenhoeft

    Citation: Plant Methods 2018 14:25

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  8. Content type: Research

    Leaf biochemical composition corresponds to traits related to the plant state and its functioning. This study puts the emphasis on the main leaf absorbers: chlorophyll a and b ( ...

    Authors: Jingyi Jiang, Alexis Comar, Philippe Burger, Pierre Bancal, Marie Weiss and Frédéric Baret

    Citation: Plant Methods 2018 14:23

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  9. Content type: Methodology

    The number of ears per unit ground area (ear density) is one of the main agronomic yield components in determining grain yield in wheat. A fast evaluation of this attribute may contribute to monitoring the eff...

    Authors: Jose A. Fernandez-Gallego, Shawn C. Kefauver, Nieves Aparicio Gutiérrez, María Teresa Nieto-Taladriz and José Luis Araus

    Citation: Plant Methods 2018 14:22

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  10. Content type: Methodology

    Unmanned aerial vehicles offer the opportunity for precision agriculture to efficiently monitor agricultural land. A vegetation index (VI) derived from an aerially observed multispectral image (MSI) can quanti...

    Authors: Zohaib Khan, Vahid Rahimi-Eichi, Stephan Haefele, Trevor Garnett and Stanley J. Miklavcic

    Citation: Plant Methods 2018 14:20

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  11. Content type: Methodology

    Virus induced gene silencing (VIGS) is a powerful genomics tool for interrogating the function of plant genes. Unfortunately, VIGS vectors often produce disease symptoms that interfere with the silencing pheno...

    Authors: C. R. Gedling, E. M. Ali, A. Gunadi, J. J. Finer, K. Xie, Y. Liu, N. Yoshikawa, F. Qu and A. E. Dorrance

    Citation: Plant Methods 2018 14:19

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  12. Content type: Methodology

    The unicellular charophycean green alga Penium margaritaceum has emerged as an appealing experimental organism in plant cell wall and cell biology research. Innovative practical approaches in the manipulation and...

    Authors: Sandra Cristina Raimundo, Iben Sørensen, Berke Tinaz, Eleanore Ritter, Jocelyn K. C. Rose and David S. Domozych

    Citation: Plant Methods 2018 14:18

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  13. Content type: Methodology

    Camellia sinensis var. sinensis is widely grown for tea beverages that possess significant health promoting effects. Studies on tea plant genetics and breeding are hindered due to its recalcitrance to Agrobacteri...

    Authors: Karthikeyan Alagarsamy, Lubobi Ferdinand Shamala and Shu Wei

    Citation: Plant Methods 2018 14:17

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  14. Content type: Methodology

    Storage starch in starchy seed influences the seed weight and texture, and determines its applications in food and nonfood industries. Starch granules from different plant sources have significantly different ...

    Authors: Lingxiao Zhao, Ting Pan, Dongwei Guo and Cunxu Wei

    Citation: Plant Methods 2018 14:16

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  15. Content type: Research

    Leaf chlorophyll content (LCC) provides valuable information about plant physiology. Most of the published chlorophyll vegetation indices at the leaf level have been based on the spectral characteristics of th...

    Authors: Shan Lu, Fan Lu, Wenqiang You, Zheyi Wang, Yu Liu and Kenji Omasa

    Citation: Plant Methods 2018 14:15

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  16. Content type: Methodology

    Lipidomics plays an important role in understanding plant adaptation to different stresses and improving our knowledge of the genes underlying lipid metabolism. Lipidomics involves lipid extraction, sample pre...

    Authors: Sunitha Shiva, Regina Enninful, Mary R. Roth, Pamela Tamura, Krishna Jagadish and Ruth Welti

    Citation: Plant Methods 2018 14:14

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  17. Content type: Research

    Stalk lodging is a serious challenge in the production of maize and sorghum. A comprehensive understanding of lodging will likely require accurate characterizations of the mechanical properties of such plants....

    Authors: Loay Al-Zube, Wenhuan Sun, Daniel Robertson and Douglas Cook

    Citation: Plant Methods 2018 14:11

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  18. Content type: Methodology

    Genome-wide characterization of tissue- or cell-specific gene expression is a recurrent bottleneck in biology. We have developed a sensitive approach based on ultra-low RNA sequencing coupled to laser assisted...

    Authors: Kaori Sakai, Ludivine Taconnat, Nero Borrega, Jennifer Yansouni, Véronique Brunaud, Christine Paysant-Le Roux, Etienne Delannoy, Marie-Laure Martin Magniette, Loïc Lepiniec, Jean Denis Faure, Sandrine Balzergue and Bertrand Dubreucq

    Citation: Plant Methods 2018 14:10

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  19. Content type: Methodology

    Due to the nature of viral RNA genomes, RNA viruses depend on many RNA-binding proteins (RBP) of viral and host origin for replication, dissemination and evasion of host RNA degradation pathways. Some viruses ...

    Authors: F. E. Marmisolle, M. L. García and C. A. Reyes

    Citation: Plant Methods 2018 14:9

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  20. Content type: Methodology

    The amino acid profile of plants is an important parameter in assessments of their growth potential, resource-use efficiency and/or quality as food and feed. Screening studies may involve large number of sampl...

    Authors: Rasmus Dahl-Lassen, Jan van Hecke, Henning Jørgensen, Christian Bukh, Birgit Andersen and Jan K. Schjoerring

    Citation: Plant Methods 2018 14:8

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  21. Content type: Methodology

    Leaf venation traits are important for many research fields such as systematics and evolutionary biology, plant physiology, climate change, and paleoecology. In spite of an increasing demand for vein trait dat...

    Authors: Julio V. Schneider, Renate Rabenstein, Jens Wesenberg, Karsten Wesche, Georg Zizka and Jörg Habersetzer

    Citation: Plant Methods 2018 14:7

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  22. Content type: Methodology

    Deep learning presents many opportunities for image-based plant phenotyping. Here we consider the capability of deep convolutional neural networks to perform the leaf counting task. Deep learning techniques ty...

    Authors: Jordan Ubbens, Mikolaj Cieslak, Przemyslaw Prusinkiewicz and Ian Stavness

    Citation: Plant Methods 2018 14:6

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  23. Content type: Methodology

    Miscanthus is a leading second generation bio-energy crop. It is mostly rhizome propagated; however, the increasing use of seed is resulting in a greater need to investigate germination. Miscanthus seed are small...

    Authors: Danny Awty-Carroll, John Clifton-Brown and Paul Robson

    Citation: Plant Methods 2018 14:5

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  24. Content type: Methodology

    Low pollen viability may limit grapevine yield under certain conditions, causing relevant economic losses to grape-growers. It is usually evaluated by the quantification of the number of viable and non-viable ...

    Authors: Javier Tello, María Ignacia Montemayor, Astrid Forneck and Javier Ibáñez

    Citation: Plant Methods 2018 14:3

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  25. Content type: Methodology

    Transient gene expression is a powerful tool to study gene function in plants. In citrus, Agrobacterium transformation is the method of choice for transient expression studies, but this method does not work effic...

    Authors: Amit Levy, Choaa El-Mochtar, Chunxia Wang, Michael Goodin and Vladimir Orbovic

    Citation: Plant Methods 2018 14:2

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  26. Content type: Software

    Plants demonstrate dynamic growth phenotypes that are determined by genetic and environmental factors. Phenotypic analysis of growth features over time is a key approach to understand how plants interact with ...

    Authors: Ji Zhou, Christopher Applegate, Albor Dobon Alonso, Daniel Reynolds, Simon Orford, Michal Mackiewicz, Simon Griffiths, Steven Penfield and Nick Pullen

    Citation: Plant Methods 2017 13:117

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  27. Content type: Methodology

    Fine-grained recognition of plants from images is a challenging computer vision task, due to the diverse appearance and complex structure of plants, high intra-class variability and small inter-class differenc...

    Authors: Milan Šulc and Jiří Matas

    Citation: Plant Methods 2017 13:115

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  28. Content type: Software

    Developmental biology has made great strides in recent years towards the quantification of cellular properties during development. This requires tissues to be imaged and segmented to generate computerised vers...

    Authors: Annamária Kiss, Typhaine Moreau, Vincent Mirabet, Cerasela Iliana Calugaru, Arezki Boudaoud and Pradeep Das

    Citation: Plant Methods 2017 13:114

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  29. Content type: Methodology

    Switchgrass (Panicum virgatum), a robust perennial C4-type grass, has been evaluated and designated as a model bioenergy crop by the U.S. DOE and USDA. Conventional breeding of switchgrass biomass is difficult be...

    Authors: Chien-Yuan Lin, Bryon S. Donohoe, Neha Ahuja, Deborah M. Garrity, Rongda Qu, Melvin P. Tucker, Michael E. Himmel and Hui Wei

    Citation: Plant Methods 2017 13:113

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  30. Content type: Methodology

    To investigate translational regulation of gene expression in plant mitochondria, a mitochondrial polysome isolation protocol was established for tobacco to investigate polysomal mRNA loading as a proxy for tr...

    Authors: Muhammad Waqar Hameed, Ilona Juszczak, Ralph Bock and Joost Thomas van Dongen

    Citation: Plant Methods 2017 13:112

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  31. Content type: Methodology

    The morphological analysis of olive leaves, fruits and endocarps may represent an efficient tool for the characterization and discrimination of cultivars and the establishment of relationships among them. In r...

    Authors: Konstantinos N. Blazakis, Maria Kosma, George Kostelenos, Luciana Baldoni, Marina Bufacchi and Panagiotis Kalaitzis

    Citation: Plant Methods 2017 13:111

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  32. Content type: Research

    The development of cell pattern in the surface cell layer of the shoot apex can be investigated in vivo by use of a time-lapse confocal images, showing naked meristem in 3D in successive times. However, how th...

    Authors: Krzysztof Kucypera, Marcin Lipowczan, Anna Piekarska-Stachowiak and Jerzy Nakielski

    Citation: Plant Methods 2017 13:110

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  33. Content type: Methodology

    Sorghum (Sorghum bicolor L.) is one of the world’s most important cereal crops grown for multiple applications and has been identified as a potential biofuel crop. Despite several decades of study, sorghum has be...

    Authors: Srinivas Belide, Thomas Vanhercke, James Robertson Petrie and Surinder Pal Singh

    Citation: Plant Methods 2017 13:109

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  34. Content type: Methodology

    Plant extracts contain a huge variety of pharmacologically active substances. Conventionally, various chromatographic methods must be applied several times to purify functional compounds to measure their funct...

    Authors: Ye Ji Park, Si Hyun Seong, Min Sun Kim, Sang Wan Seo, Mee Ree Kim and Hyun Sik Kim

    Citation: Plant Methods 2017 13:108

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  35. Content type: Methodology

    The proportion of granule types in wheat starch is an important characteristic that can affect its functionality. It is widely accepted that granule types are either large, disc-shaped A-type granules or small...

    Authors: Emi Tanaka, Jean-Phillippe F. Ral, Sean Li, Raj Gaire, Colin R. Cavanagh, Brian R. Cullis and Alex Whan

    Citation: Plant Methods 2017 13:107

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  36. Content type: Methodology

    Several high-throughput molecular genetic analyses rely on high-quality genomic DNA. Copurification of other molecules can negatively impact the functionality of plant DNA preparations employed in these proced...

    Authors: Kelvin T. Chiong, Mona B. Damaj, Carmen S. Padilla, Carlos A. Avila, Shankar R. Pant, Kranthi K. Mandadi, Ninfa R. Ramos, Denise V. Carvalho and T. Erik Mirkov

    Citation: Plant Methods 2017 13:106

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  37. Content type: Research

    Rice panicle phenotyping is important in rice breeding, and rice panicle segmentation is the first and key step for image-based panicle phenotyping. Because of the challenge of illumination differentials, pani...

    Authors: Xiong Xiong, Lingfeng Duan, Lingbo Liu, Haifu Tu, Peng Yang, Dan Wu, Guoxing Chen, Lizhong Xiong, Wanneng Yang and Qian Liu

    Citation: Plant Methods 2017 13:104

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  38. Content type: Research

    Accurately segmenting vegetation from the background within digital images is both a fundamental and a challenging task in phenotyping. The performance of traditional methods is satisfactory in homogeneous env...

    Authors: Pouria Sadeghi-Tehran, Nicolas Virlet, Kasra Sabermanesh and Malcolm J. Hawkesford

    Citation: Plant Methods 2017 13:103

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  39. Content type: Methodology

    Root systems are highly plastic and adapt according to their soil environment. Studying the particular influence of soils on root development necessitates the adaptation and evaluation of imaging methods for m...

    Authors: Daniel Pflugfelder, Ralf Metzner, Dagmar van Dusschoten, Rüdiger Reichel, Siegfried Jahnke and Robert Koller

    Citation: Plant Methods 2017 13:102

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  40. Content type: Correction

    After publication of this article [1], the authors noted the following error. Due to a calculation error in the temperature term f(TL) of the emission standardization algorithm (Eq. 3 of the original paper), the ...

    Authors: Marvin Lüpke, Rainer Steinbrecher, Michael Leuchner and Annette Menzel

    Citation: Plant Methods 2017 13:100

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    The original article was published in Plant Methods 2017 13:14

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