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  1. NADPH oxidase (Nox) plays a crucial role in reactive oxygen spices (ROS) production and mediates different diseases’ development. Under aerobic conditions, the NADPH-containing protein component of the (NCP)-F...

    Authors: Sona M. Feschyan, Ruzan M. Simonyan, Gegham M. Simonyan, Maxim A. Simonyan and Ashkhen L. Manukyan
    Citation: Plant Methods 2023 19:1
  2. In June 2020, severe symptoms of leaf mosaic and fruit malformation were observed on greenhouse-grown cucumber plants in Xizhou Township of Changhua County, Taiwan. An unknown virus, designated CX-2, was isola...

    Authors: Zi-Xuan Dong, Chian-Chi Lin, Yuh-Kun Chen, Chia-Cheng Chou and Tsung-Chi Chen
    Citation: Plant Methods 2022 18:143
  3. Camellia oleifera (C. oleifera) is a woody edible oil crop of great economic importance. Because of the lack of modern biotechnology research, C. oleifera faces huge challenges in both breeding and basic researc...

    Authors: Sufang Li, Rui Zhao, Tianwen Ye, Rui Guan, Linjie Xu, Xiaoling Ma, Jiaxi Zhang, Shixin Xiao and Deyi Yuan
    Citation: Plant Methods 2022 18:141
  4. “Herbarium X-ray Fluorescence (XRF) Ionomics” is a new quantitative approach for extracting the elemental concentrations from herbarium specimens using handheld XRF devices. These instruments are principally d...

    Authors: Imam Purwadi, Lachlan W. Casey, Chris G. Ryan, Peter D. Erskine and Antony van der Ent
    Citation: Plant Methods 2022 18:139
  5. Virtual plants can simulate the plant growth and development process through computer modeling, which assists in revealing plant growth and development patterns. Virtual plant visualization technology is a cor...

    Authors: Lingfeng Duan, Zhihao Wang, Hongfei Chen, Jinyang Fu, Hanzhi Wei, Zedong Geng and Wanneng Yang
    Citation: Plant Methods 2022 18:138
  6. Long-read sequencing platforms offered by Oxford Nanopore Technologies (ONT) allow native DNA containing epigenetic modifications to be directly sequenced, but can be limited by lower per-base accuracies. A ke...

    Authors: Scott Ferguson, Todd McLay, Rose L. Andrew, Jeremy J. Bruhl, Benjamin Schwessinger, Justin Borevitz and Ashley Jones
    Citation: Plant Methods 2022 18:137
  7. Frequency is essential in signal transmission, especially in convolutional neural networks. It is vital to maintain the signal frequency in the neural network to maintain the performance of a convolutional neu...

    Authors: Jiayu Zhang, Chao Qi, Peter Mecha, Yi Zuo, Zongyou Ben, Haolu Liu and Kunjie Chen
    Citation: Plant Methods 2022 18:136
  8. Cyst nematodes are one of the major groups of plant-parasitic nematode, responsible for considerable crop losses worldwide. Improving genetic resources, and therefore resistant cultivars, is an ongoing focus o...

    Authors: Olaf Prosper Kranse, Itsuhiro Ko, Roberta Healey, Unnati Sonawala, Siyuan Wei, Beatrice Senatori, Francesco De Batté, Ji Zhou and Sebastian Eves-van den Akker
    Citation: Plant Methods 2022 18:134
  9. Root system architecture (RSA) is an essential characteristic for efficient water and nutrient absorption in terrestrial plants; its plasticity enables plants to respond to different soil environments. Better ...

    Authors: Shota Teramoto and Yusaku Uga
    Citation: Plant Methods 2022 18:133
  10. Copy number determination is one of the first steps in the characterization of transgenic plant lines. The classical approach to this, Southern blotting, is time-consuming, expensive and requires massive amoun...

    Authors: Jonas De Saeger, Jihae Park, Kai Thoris, Charlotte De Bruyn, Hoo Sun Chung, Dirk Inzé and Stephen Depuydt
    Citation: Plant Methods 2022 18:132
  11. Seedling growth is an early phase of plant development highly susceptible to environmental factors such as soil nitrogen (N) availability or presence of seed-borne pathogens. Whereas N plays a central role in ...

    Authors: Thibault Barrit, Claire Campion, Sophie Aligon, Julie Bourbeillon, David Rousseau, Elisabeth Planchet and Béatrice Teulat
    Citation: Plant Methods 2022 18:131
  12. High-throughput phenotyping platforms allow the study of the form and function of a large number of genotypes subjected to different growing conditions (GxE). A number of image acquisition and processing pipel...

    Authors: Benoit Daviet, Romain Fernandez, Llorenç Cabrera-Bosquet, Christophe Pradal and Christian Fournier
    Citation: Plant Methods 2022 18:130
  13. The technology of cotton defoliation is essential for mechanical cotton harvesting. Agricultural unmanned aerial vehicle (UAV) spraying has the advantages of low cost, high efficiency and no mechanical damage ...

    Authors: Jinyong Wu, Sheng Wen, Yubin Lan, Xuanchun Yin, Jiantao Zhang and Yufeng Ge
    Citation: Plant Methods 2022 18:129
  14. As complete and accurate genome sequences are becoming easier to obtain, more researchers wish to get one or more of them to support their research endeavors. Reliable and well-documented sequence assembly wor...

    Authors: Marina Püpke Marone, Harmeet Chawla Singh, Curtis J. Pozniak and Martin Mascher
    Citation: Plant Methods 2022 18:128
  15. High-throughput phenotyping is crucial for the genetic and molecular understanding of adaptive root system development. In recent years, imaging automata have been developed to acquire the root system architec...

    Authors: Romain Fernandez, Amandine Crabos, Morgan Maillard, Philippe Nacry and Christophe Pradal
    Citation: Plant Methods 2022 18:127
  16. Our understanding of the physiological responses of rice inflorescence (panicle) to environmental stresses is limited by the challenge of accurately determining panicle photosynthetic parameters and their impa...

    Authors: Jaspinder Singh Dharni, Balpreet Kaur Dhatt, Puneet Paul, Tian Gao, Tala Awada, Harel Bacher, Zvi Peleg, Paul Staswick, Jason Hupp, Hongfeng Yu and Harkamal Walia
    Citation: Plant Methods 2022 18:126
  17. Grasses internodes are made of distinct tissues such as vascular bundles, epidermis, rind and pith. The histology of grasses stem was largely revisited recently taking advantage of the development of microscop...

    Authors: P.-L. Lopez-Marnet, S. Guillaume, V. Méchin and M. Reymond
    Citation: Plant Methods 2022 18:125
  18. Chlorophyll and carotenoids, the most widely distributed lipophilic pigments in plants, contribute to fruit coloration during development and ripening. These pigments are assembled with pigment-protein complex...

    Authors: Jinli Gong, Hang Zhang, Yunliu Zeng, Yunjiang Cheng, Xuepeng Sun and Pengwei Wang
    Citation: Plant Methods 2022 18:124
  19. Acidic phytohormones are small molecules controlling many physiological functions in plants. A comprehensive picture of their profiles including the active forms, precursors and metabolites provides an importa...

    Authors: Jitka Široká, Federica Brunoni, Aleš Pěnčík, Václav Mik, Asta Žukauskaitė, Miroslav Strnad, Ondřej Novák and Kristýna Floková
    Citation: Plant Methods 2022 18:122
  20. Commonly, several traits are assessed in agronomic experiments to better understand the factors under study. However, it is also common to see that even when several traits are available, researchers opt to fo...

    Authors: Tiago Olivoto, Maria I. Diel, Denise Schmidt and Alessandro D. Lúcio
    Citation: Plant Methods 2022 18:121
  21. Cytokinins are a class of phytohormone that play a crucial role in the development of plants. They are involved in the regulation of nearly every aspect of plant growth, from germination to senescence. The rol...

    Authors: Lachlan Dow, Russell A. Barrow, Rosemary G. White and Ulrike Mathesius
    Citation: Plant Methods 2022 18:120
  22. Phytochemicals or secondary metabolites are low molecular weight organic compounds with little function in plant growth and development. Nevertheless, the metabolite diversity govern not only the phenetics of ...

    Authors: Muhammad-Redha Abdullah-Zawawi, Nisha Govender, Mohammad Bozlul Karim, Md. Altaf-Ul-Amin, Shigehiko Kanaya and Zeti-Azura Mohamed-Hussein
    Citation: Plant Methods 2022 18:118
  23. Researchers interested in the seed size of rapeseed need to quantify the cell size and number of cells in the seed coat, embryo and silique wall. Scanning electron microscope-based methods have been demonstrat...

    Authors: Yushun Jiao, Baoling Liang, Guangsheng Yang, Qiang Xin and Dengfeng Hong
    Citation: Plant Methods 2022 18:117
  24. Soybean gene functions cannot be easily interrogated through transgenic disruption (knock-out) of genes-of-interest, or transgenic overexpression of proteins-of-interest, because soybean transformation is time...

    Authors: Fides Angeli Zaulda, Seung Hyun Yang, Junping Han, Sizolwenkosi Mlotshwa, Anne Dorrance and Feng Qu
    Citation: Plant Methods 2022 18:116
  25. Living cells maintain and adjust structural and functional integrity by continual synthesis and degradation of metabolites and macromolecules. The maintenance and adjustment of thylakoid membrane involve turno...

    Authors: Anh Thi-Mai Banh, Björn Thiele, Antonia Chlubek, Thomas Hombach, Einhard Kleist and Shizue Matsubara
    Citation: Plant Methods 2022 18:114
  26. As one of the most widely planted fruit trees in southern China, citrus occupies an important position in the agriculture field and forestry economy in China. There are many kinds of citrus diseases. If citrus...

    Authors: Ruoli Yang, Tingjing Liao, Peirui Zhao, Wenhua Zhou, Mingfang He and Liujun Li
    Citation: Plant Methods 2022 18:113
  27. Defining interactions of bacteria in the rhizosphere (encompassing the area near and on the plant root) is important to understand how they affect plant health. Some rhizosphere bacteria, including plant growt...

    Authors: Amanda Rosier and Harsh P. Bais
    Citation: Plant Methods 2022 18:112
  28. According to the most popular definition, a biostimulant is any substance or microorganism applied to plants with the aim to enhance nutrition efficiency, abiotic stress tolerance and/or crop quality traits, r...

    Authors: Patricia Benito, Daniele Ligorio, Javier Bellón, Lynne Yenush and José M. Mulet
    Citation: Plant Methods 2022 18:111
  29. Phenomic prediction has been defined as an alternative to genomic prediction by using spectra instead of molecular markers. A reflectance spectrum provides information on the biochemical composition within a t...

    Authors: Charlotte Brault, Juliette Lazerges, Agnès Doligez, Miguel Thomas, Martin Ecarnot, Pierre Roumet, Yves Bertrand, Gilles Berger, Thierry Pons, Pierre François, Loïc Le Cunff, Patrice This and Vincent Segura
    Citation: Plant Methods 2022 18:108
  30. The allocation of non-structural carbohydrates (NSCs) plays a critical role in the physiology and metabolism of tree growth and survival defense. However, little is known about the allocation of NSC after cont...

    Authors: Yanjie Li, Honggang Sun, Thiago de Paula Protásio, Paulo Ricardo Gherardi Hein and Baoguo Du
    Citation: Plant Methods 2022 18:107
  31. Cowpea is a dryland crop with potential to improve food security in sub-Saharan Africa, where it is mostly produced and consumed. Contemporary plant improvement technologies, including genome editing, marker-a...

    Authors: Offiong Ukpong Edet and Takayoshi Ishii
    Citation: Plant Methods 2022 18:106
  32. Genome complexity is largely linked to diversification and crop innovation. Examples of regions with duplicated genes with relevant roles in agricultural traits are found in many crops. In both duplicated and ...

    Authors: Arnau Fiol, Federico Jurado-Ruiz, Elena López‑Girona and Maria José Aranzana
    Citation: Plant Methods 2022 18:105
  33. Eucommia ulmoides leaf (EUL), as a medicine and food homology plant, is a high-quality industrial raw material with great development potential for a valuable economic crop. There are many factors affecting the q...

    Authors: Lian Li, Zhi Min Li and Yuan Zhong Wang
    Citation: Plant Methods 2022 18:102
  34. As a rapid and non-destructive method, Near Infrared Spectroscopy is classically proposed to assess plant traits in many scientific fields, to observe enlarged genotype panels and to document the temporal kine...

    Authors: Héloïse Villesseche, Martin Ecarnot, Elsa Ballini, Ryad Bendoula, Nathalie Gorretta and Pierre Roumet
    Citation: Plant Methods 2022 18:100
  35. Quantification of gene expression such as RNA-Seq is a popular approach to study various biological phenomena. Despite the development of RNA-Seq library preparation methods and sequencing platforms in the las...

    Authors: Makoto Kashima, Mari Kamitani, Yasuyuki Nomura, Natsumi Mori-Moriyama, Shigeyuki Betsuyaku, Hiromi Hirata and Atsushi J. Nagano
    Citation: Plant Methods 2022 18:99
  36. Genome editing is essential for crop molecular breeding. However, gene editing in turnip (Brassica rapa var. rapa) have not been reported owing to the very low transformation efficiency.

    Authors: Yuanyuan Liu, Li Zhang, Cheng Li, Yunqiang Yang, Yuanwen Duan, Yongping Yang and Xudong Sun
    Citation: Plant Methods 2022 18:98
  37. Photosynthesis close interacts with respiration and nitrogen assimilation, which determine the photosynthetic efficiency of a leaf. Accurately quantifying the metabolic fluxes in photosynthesis, respiration an...

    Authors: Qiming Tang, Qingfeng Song, Xiaoxiang Ni, Zai Shi, Genyun Chen and Xinguang Zhu
    Citation: Plant Methods 2022 18:97
  38. Characterizing plant genetic resources and their response to the environment through accurate measurement of relevant traits is crucial to genetics and breeding. Spatial organization of the maize ear provides ...

    Authors: V. Oury, T. Leroux, O. Turc, R. Chapuis, C. Palaffre, F. Tardieu, S. Alvarez Prado, C. Welcker and S. Lacube
    Citation: Plant Methods 2022 18:96

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