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  1. The construction and application of synthetic genetic circuits is frequently improved if gene expression can be orthogonally controlled, relative to the host. In plants, orthogonality can be achieved via the u...

    Authors: Shaunak Kar, Yogendra Bordiya, Nestor Rodriguez, Junghyun Kim, Elizabeth C. Gardner, Jimmy D. Gollihar, Sibum Sung and Andrew D. Ellington
    Citation: Plant Methods 2022 18:42
  2. Elucidating the genetic and molecular control of plant reproduction often requires the deployment of functional approaches based on reverse or forward genetic screens. The loss-of-function of essential genes, ...

    Authors: Jasmin Schubert, Yanru Li, Marta A. Mendes, Danli Fei, Hugh Dickinson, Ian Moore and Célia Baroux
    Citation: Plant Methods 2022 18:41
  3. Stable isotope labeling is a non-invasive, sensitive means of monitoring metabolic flux in plants. The most physiologically meaningful information is obtained from experiments that take advantage of the natura...

    Authors: Sonia E. Evans, Peter Duggan, Matthew E. Bergman, Daniela Cobo-López, Benjamin Davis, Ibadat Bajwa and Michael A. Phillips
    Citation: Plant Methods 2022 18:40
  4. Roots are vital to plant performance because they acquire resources from the soil and provide anchorage. However, it remains difficult to assess root system size and distribution because roots are inaccessible...

    Authors: Ashish B. Rajurkar, Scott M. McCoy, Jeremy Ruhter, Jessica Mulcrone, Luke Freyfogle and Andrew D. B. Leakey
    Citation: Plant Methods 2022 18:39
  5. Profiling the plant root architecture is vital for selecting resilient crops that can efficiently take up water and nutrients. The high-performance imaging tools available to study root-growth dynamics with th...

    Authors: Vinicius Lube, Mehmet Alican Noyan, Alexander Przybysz, Khaled Salama and Ikram Blilou
    Citation: Plant Methods 2022 18:38
  6. Plant DNA isolation and purification is a time-consuming and laborious process relative to epithelial and viral DNA sample preparation due to the cell wall. The lysis of plant cells to free intracellular DNA n...

    Authors: Miranda N. Emaus, Cecilia Cagliero, Morgan R. Gostel, Gabriel Johnson and Jared L. Anderson
    Citation: Plant Methods 2022 18:37
  7. Soybean iron deficiency chlorosis (IDC) is an important nutrient stress frequently found in high pH and/or soils high in calcium carbonates. To advance the understanding of IDC resistance in soybean, a rapid (...

    Authors: R. Merry, M. J. Espina, A. J. Lorenz and R. M. Stupar
    Citation: Plant Methods 2022 18:36
  8. Plant pathogens cause yield losses in crops worldwide. Breeding for improved disease resistance and management by precision agriculture are two approaches to limit such yield losses. Both rely on detecting and...

    Authors: Florian Tanner, Sebastian Tonn, Jos de Wit, Guido Van den Ackerveken, Bettina Berger and Darren Plett
    Citation: Plant Methods 2022 18:35
  9. In addition to heterogeneity and artificial selection, natural selection is one of the forces used to combat climate change and improve agrobiodiversity in evolutionary plant breeding. Accurate identification ...

    Authors: Michael Schneider, Asis Shrestha, Agim Ballvora and Jens Léon
    Citation: Plant Methods 2022 18:34
  10. The genetic engineering of crops has enhanced productivity in the face of climate change and a growing global population by conferring desirable genetic traits, including the enhancement of biotic and abiotic ...

    Authors: Lamiaa M. Mahmoud, Prabhjot Kaur, Daniel Stanton, Jude W. Grosser and Manjul Dutt
    Citation: Plant Methods 2022 18:33
  11. Traditional CRISPR/Cas9 systems that rely on U6 or U3 snRNA promoters (RNA polymerase III-dependent promoters) can only achieve constitutive gene editing in plants, hampering the functional analysis of specifical...

    Authors: Jia-Wen Li, Tuo Zeng, Zhi-Zhuo Xu, Jin-Jin Li, Hao Hu, Qin Yu, Li Zhou, Ri-Ru Zheng, Jing Luo and Cai-Yun Wang
    Citation: Plant Methods 2022 18:32
  12. In order to improve the current situations in rice–wheat rotation region in the middle and lower reaches of the Yangtze River, such as large amount of rice straw, complex returning process, short-time stubble ...

    Authors: Yinyan Shi, Ye Jiang, Xiaochan Wang, Haiming Yu, Hui Liu and Jingbo Chen
    Citation: Plant Methods 2022 18:31
  13. High-throughput plant phenotyping (HTPP) methods have the potential to speed up the crop breeding process through the development of cost-effective, rapid and scalable phenotyping methods amenable to automatio...

    Authors: Alexander Koc, Firuz Odilbekov, Marwan Alamrani, Tina Henriksson and Aakash Chawade
    Citation: Plant Methods 2022 18:30
  14. With the rise of artificial intelligence, deep learning is gradually applied to the field of agriculture and plant science. However, the excellent performance of deep learning needs to be established on massiv...

    Authors: Jiachen Yang, Xiaolan Guo, Yang Li, Francesco Marinello, Sezai Ercisli and Zhuo Zhang
    Citation: Plant Methods 2022 18:28
  15. Faba bean is an important legume crop in the world. Plant height and yield are important traits for crop improvement. The traditional plant height and yield measurement are labor intensive and time consuming. ...

    Authors: Yishan Ji, Zhen Chen, Qian Cheng, Rong Liu, Mengwei Li, Xin Yan, Guan Li, Dong Wang, Li Fu, Yu Ma, Xiuliang Jin, Xuxiao Zong and Tao Yang
    Citation: Plant Methods 2022 18:26
  16. Although there have been numerous studies describing plant growth systems for root exudate collection, a common limitation is that these systems require disruption of the plant root system to facilitate exudat...

    Authors: Martha G. Lopez-Guerrero, Peng Wang, Felicia Phares, Daniel P. Schachtman, Sophie Alvarez and Karin van Dijk
    Citation: Plant Methods 2022 18:25
  17. Grafting is a technique widely used in horticulture that also has been applied in agriculture. In plant physiology, grafting facilitates the elucidation of mechanisms underlying growth and developmental proces...

    Authors: L. Vanderstraeten, R. Sanchez-Muñoz, T. Depaepe, F. Auwelaert and D. Van Der Straeten
    Citation: Plant Methods 2022 18:24
  18. Blue Native polyacrylamide gel electrophoresis (BN PAGE) followed by denaturing PAGE is a widely used, convenient and time efficient method to separate thylakoid complexes and study their composition, abundanc...

    Authors: Éva Sárvári, Gabriella Gellén, Máté Sági-Kazár, Gitta Schlosser, Katalin Solymosi and Ádám Solti
    Citation: Plant Methods 2022 18:23
  19. Although the spectrum of effective methods and techniques that allow determination of inorganic or total phosphorus is impressive, more precise analysis of these substances in plant tissues is not a routine or...

    Authors: Dorota Wieczorek, Beata Żyszka-Haberecht, Anna Kafka and Jacek Lipok
    Citation: Plant Methods 2022 18:22
  20. In recent years, there has been an increase of interest in plant behaviour as represented by growth-driven responses. These are generally classified into nastic (internally driven) and tropic (environmentally ...

    Authors: Gabriella E. C. Gall, Talmo D. Pereira, Alex Jordan and Yasmine Meroz
    Citation: Plant Methods 2022 18:21
  21. The application of ribo nucleic acids for molecular studies requires high integrity and quality of extracted total RNA samples. In addition, the need to transfer RNA samples at room temperature without special...

    Authors: Leila Mirzaei, Abbas Yadollahi, Maryam Jafarkhani Kermani, Masoud Naderpour and Ali Asghar Zeinanloo
    Citation: Plant Methods 2022 18:19
  22. Some plastid-derived metabolites can control nuclear gene expression, chloroplast biogenesis, and chlorophyll biosynthesis. For example, norflurazon (NFZ) induced inhibition of carotenoid biosynthesis in leave...

    Authors: N. Dhami, B. J. Pogson, D. T. Tissue and C. I. Cazzonelli
    Citation: Plant Methods 2022 18:18
  23. Layered double hydroxide lactate nanosheets (LDH-lactate-NS) are powerful carriers for delivering macro-molecules into intact plant cells. In the past few years, some studies have been carried out on DNA/RNA t...

    Authors: Hongyang Wu, He Zhang, Xinyu Li, Yu Zhang, Jiankun Wang, Qiang Wang and Yinglang Wan
    Citation: Plant Methods 2022 18:17
  24. RNA live-cell imaging systems have been used to visualize subcellular mRNA distribution in living cells. The RNA-binding protein (RBP)-based RNA imaging system exploits specific RBP and the corresponding RNA r...

    Authors: Nien-Chen Huang, Kai-Ren Luo and Tien-Shin Yu
    Citation: Plant Methods 2022 18:15
  25. Developing a systematic phenotypic data analysis pipeline, creating enhanced visualizations, and interpreting the results is crucial to extract meaningful insights from data in making better breeding decisions...

    Authors: Waseem Hussain, Mahender Anumalla, Margaret Catolos, Apurva Khanna, Ma. Teresa Sta. Cruz, Joie Ramos and Sankalp Bhosale
    Citation: Plant Methods 2022 18:14
  26. Precision agriculture techniques are widely used to optimize fertilizer and soil applications. Furthermore, these techniques could also be combined with new statistical tools to assist in phenotyping in breedi...

    Authors: Dthenifer Cordeiro Santana, Marcos Paulo de Oliveira Cunha, Regimar Garcia dos Santos, Mayara Fávero Cotrim, Larissa Pereira Ribeiro Teodoro, Carlos Antonio da Silva Junior, Fabio Henrique Rojo Baio and Paulo Eduardo Teodoro
    Citation: Plant Methods 2022 18:13
  27. Rosette leaf trichomes of Arabidopsis thaliana have been broadly used to study cell development, cell differentiation and, more recently, cell wall biogenesis. However, trichome-specific biochemical or -omics ana...

    Authors: Jan W. Huebbers, Kim Büttgen, Franz Leissing, Melissa Mantz, Markus Pauly, Pitter F. Huesgen and Ralph Panstruga
    Citation: Plant Methods 2022 18:12
  28. Plant plasma membrane transporters play essential roles during the translocation of vectorized agrochemicals. Therefore, transporters associated with phloem loading of vectorized agrochemicals have drawn incre...

    Authors: Yongxin Xiao, Jinying Zhang, Yiting Li, Tom Hsiang, Xingping Zhang, Yongxing Zhu, Xiaoying Du, Junliang Yin and Junkai Li
    Citation: Plant Methods 2022 18:11
  29. Dendrobium nobile Lindl. is an important pharmacopeial plant with medicinal and ornamental value. This study sought to provide a technical means for the large-scale production of total alkaloid in D. nobile. Seed...

    Authors: Benhou Zhang, Zhitao Niu, Chao Li, Zhenyu Hou, Qingyun Xue, Wei Liu and Xiaoyu Ding
    Citation: Plant Methods 2022 18:10
  30. Rice is a major staple food crop for more than half the world’s population. As the global population is expected to reach 9.7 billion by 2050, increasing the production of high-quality rice is needed to meet t...

    Authors: Chaoxin Wang, Doina Caragea, Nisarga Kodadinne Narayana, Nathan T. Hein, Raju Bheemanahalli, Impa M. Somayanda and S. V. Krishna Jagadish
    Citation: Plant Methods 2022 18:9
  31. Leaf hairiness (pubescence) is an important plant phenotype which regulates leaf transpiration, affects sunlight penetration, and provides increased resistance or susceptibility against certain insects. Cotton...

    Authors: Vivien Rolland, Moshiur R. Farazi, Warren C. Conaty, Deon Cameron, Shiming Liu, Lars Petersson and Warwick N. Stiller
    Citation: Plant Methods 2022 18:8
  32. Ralstonia solanacearum, one of the most devastating bacterial plant pathogens, is the causal agent of bacterial wilt. Recently, several studies on resistance to bacterial wilt have been conducted using the Arabid...

    Authors: Cuihong Xu, Lingkun Zhong, Zeming Huang, Chenying Li, Jiazhang Lian, Xuefang Zheng and Yan Liang
    Citation: Plant Methods 2022 18:7
  33. The incorporation of root traits into elite germplasm is typically a slow process. Thus, innovative approaches are required to accelerate research and pre-breeding programs targeting root traits to improve yie...

    Authors: Charlotte Rambla, Sarah Van Der Meer, Kai P. Voss-Fels, Manar Makhoul, Christian Obermeier, Rod Snowdon, Eric S. Ober, Michelle Watt, Samir Alahmad and Lee T. Hickey
    Citation: Plant Methods 2022 18:2
  34. Lupins are promising protein crops with an increasing amount of genomic and transcriptomic resources. The new resources facilitate the in silico identification of candidate genes controlling important agronomi...

    Authors: Davide Mancinotti, Maria Cecilia Rodriguez, Karen Michiko Frick, Bjørn Dueholm, Ditte Goldschmidt Jepsen, Niels Agerbirk and Fernando Geu-Flores
    Citation: Plant Methods 2021 17:131
  35. With increasing joint research cooperation on national and international levels, there is a high need for harmonized and reproducible cultivation conditions and experimental protocols in order to ensure the be...

    Authors: Paul Grünhofer, Yayu Guo, Ruili Li, Jinxing Lin and Lukas Schreiber
    Citation: Plant Methods 2021 17:129
  36. The leaf epidermis functions to prevent the loss of water and reduce gas exchange. As an interface between the plant and its external environment, it helps prevent damage, making it an attractive system for st...

    Authors: Jieping Li, Xinlei Feng and Jinjin Xie
    Citation: Plant Methods 2021 17:128
  37. 3D imaging, such as X-ray CT and MRI, has been widely deployed to study plant root structures. Many computational tools exist to extract coarse-grained features from 3D root images, such as total volume, root ...

    Authors: Dan Zeng, Mao Li, Ni Jiang, Yiwen Ju, Hannah Schreiber, Erin Chambers, David Letscher, Tao Ju and Christopher N. Topp
    Citation: Plant Methods 2021 17:127
  38. The bacterial leaf pathogen Pseudomonas syringae pv tomato (Pst) is the most popular model pathogen for plant pathology research. Previous methods to study the plant-Pst interactions rely on destructive quantific...

    Authors: Leonardo Furci, David Pascual-Pardo and Jurriaan Ton
    Citation: Plant Methods 2021 17:126
  39. The root system architecture (RSA) of alfalfa (Medicago sativa L.) affects biomass production by influencing water and nutrient uptake, including nitrogen fixation. Further, roots are important for storing carboh...

    Authors: Bruna Bucciarelli, Zhanyou Xu, Samadangla Ao, Yuanyuan Cao, Maria J. Monteros, Christopher N. Topp and Deborah A. Samac
    Citation: Plant Methods 2021 17:125
  40. Ribo-seq has revolutionized the study of genome-wide mRNA translation. High-quality Ribo-seq data display strong 3-nucleotide (nt) periodicity, which corresponds to translating ribosomes deciphering three nts ...

    Authors: Hsin-Yen Larry Wu and Polly Yingshan Hsu
    Citation: Plant Methods 2021 17:124

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