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  1. Content type: Review

    Chloroplasts play a great role for sustained wellbeing of life on the planet. They have the power and raw materials that can be used as sophisticated biological factories. They are rich in energy as they have ...

    Authors: Muhamed Adem, Dereje Beyene and Tileye Feyissa

    Citation: Plant Methods 2017 13:30

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  2. Content type: Methodology

    Sugarcane (Saccharum spp.) is the main raw material for sugar and ethanol production. Among the abiotic stress, drought is the main one that negatively impact sugarcane yield. Although gene expression analysis th...

    Authors: Larissa Mara de Andrade, Michael dos Santos Brito, Rafael Fávero Peixoto Junior, Paulo Eduardo Ribeiro Marchiori, Paula Macedo Nóbile, Alexandre Palma Boer Martins, Rafael Vasconcelos Ribeiro and Silvana Creste

    Citation: Plant Methods 2017 13:28

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  3. Content type: Methodology

    Endoreduplication, the process of DNA replication in the absence of cell division, is associated with specialized cellular function and increased cell size. Genes controlling endoreduplication in tomato fruit ...

    Authors: F. Parker E. Laimbeer, Sarah H. Holt, Melissa Makris, Michael Alan Hardigan, C. Robin Buell and Richard E. Veilleux

    Citation: Plant Methods 2017 13:27

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  4. Content type: Methodology

    Chlorophyll content decreases in plants under stress conditions, therefore it is used commonly as an indicator of plant health. Arabidopsis thaliana offers a convenient and fast way to test physiological phenotyp...

    Authors: Ying Liang, Daisuke Urano, Kang-Ling Liao, Tyson L. Hedrick, Yajun Gao and Alan M. Jones

    Citation: Plant Methods 2017 13:26

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  5. Content type: Methodology

    Peanut (Arachis hypogaea) production is largely affected by a variety of abiotic and biotic stresses, including the root-knot nematode (RKN) Meloidogyne arenaria that causes yield losses worldwide. Transcriptome ...

    Authors: Larissa Arrais Guimaraes, Bruna Medeiros Pereira, Ana Claudia Guerra Araujo, Patricia Messenberg Guimaraes and Ana Cristina Miranda Brasileiro

    Citation: Plant Methods 2017 13:25

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  6. Content type: Methodology

    Phenotyping is a critical component of plant research. Accurate and precise trait collection, when integrated with genetic tools, can greatly accelerate the rate of genetic gain in crop improvement. However, e...

    Authors: Hsiang Sing Naik, Jiaoping Zhang, Alec Lofquist, Teshale Assefa, Soumik Sarkar, David Ackerman, Arti Singh, Asheesh K. Singh and Baskar Ganapathysubramanian

    Citation: Plant Methods 2017 13:23

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  7. Content type: Methodology

    Random insertional mutagenesis of Chlamydomonas reinhardtii using drug resistance cassettes has contributed to the generation of tens of thousands of transformants in dozens of labs around the world. In many inst...

    Authors: Steve V. Pollock, Bratati Mukherjee, Joanna Bajsa-Hirschel, Marylou C. Machingura, Ananya Mukherjee, Arthur R. Grossman and James V. Moroney

    Citation: Plant Methods 2017 13:22

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  8. Content type: Methodology

    The maize male inflorescence (tassel) produces pollen necessary for reproduction and commercial grain production of maize. The size of the tassel has been linked to factors affecting grain yield, so understand...

    Authors: Joseph L. Gage, Nathan D. Miller, Edgar P. Spalding, Shawn M. Kaeppler and Natalia de Leon

    Citation: Plant Methods 2017 13:21

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  9. Content type: Research

    The liverwort Marchantia polymorpha occupies a crucial position in land plant evolution and provides the opportunity to investigate adaptations to a terrestrial plant life style. Marchantia reverse genetic analys...

    Authors: Sarah Kopischke, Esther Schüßler, Felix Althoff and Sabine Zachgo

    Citation: Plant Methods 2017 13:20

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  10. Content type: Research

    Cytoskeleton can be observed in live plant cells in situ with high spatial and temporal resolution using a combination of specific fluorescent protein tag expression and advanced microscopy methods such as spi...

    Authors: Fatima Cvrčková and Denisa Oulehlová

    Citation: Plant Methods 2017 13:19

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  11. Content type: Methodology

    Smarthouses capable of non-destructive, high-throughput plant phenotyping collect large amounts of data that can be used to understand plant growth and productivity in extreme environments. The challenge is to...

    Authors: Rui Meng, Stephanie Saade, Sebastian Kurtek, Bettina Berger, Chris Brien, Klaus Pillen, Mark Tester and Ying Sun

    Citation: Plant Methods 2017 13:18

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  12. Content type: Methodology

    Glucosinolates, anionic sulfur rich secondary metabolites, have been extensively studied because of their occurrence in the agriculturally important brassicaceae and their impact on human and animal health. Th...

    Authors: T. Doheny-Adams, K. Redeker, V. Kittipol, I. Bancroft and S. E. Hartley

    Citation: Plant Methods 2017 13:17

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  13. Content type: Methodology

    Mitochondrial respiration in the dark (R dark) is a critical plant physiological process, and hence a reliable, efficient and high-throughput method of measuring variation in rates of R

    Authors: Andrew P. Scafaro, A. Clarissa A. Negrini, Brendan O’Leary, F. Azzahra Ahmad Rashid, Lucy Hayes, Yuzhen Fan, You Zhang, Vincent Chochois, Murray R. Badger, A. Harvey Millar and Owen K. Atkin

    Citation: Plant Methods 2017 13:16

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  14. Content type: Methodology

    Robust segmentation of canopy cover (CC) from large amounts of images taken under different illumination/light conditions in the field is essential for high throughput field phenotyping (HTFP). We attempted to...

    Authors: Kang Yu, Norbert Kirchgessner, Christoph Grieder, Achim Walter and Andreas Hund

    Citation: Plant Methods 2017 13:15

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  15. Content type: Methodology

    Biogenic volatile organic compounds (BVOC) emitted by plants play an important role for ecological and physiological processes, for example as response to stressors. These emitted compounds are involved in che...

    Authors: Marvin Lüpke, Rainer Steinbrecher, Michael Leuchner and Annette Menzel

    Citation: Plant Methods 2017 13:14

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    The Correction to this article has been published in Plant Methods 2017 13:100

  16. Content type: Methodology

    Growth is an important parameter to consider when studying the impact of treatments or mutations on plant physiology. Leaf area and growth rates can be estimated efficiently from images of plants, but the expe...

    Authors: Filipa Tomé, Karel Jansseune, Bernadette Saey, Jack Grundy, Korneel Vandenbroucke, Matthew A. Hannah and Henning Redestig

    Citation: Plant Methods 2017 13:13

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  17. Content type: Software

    Computer-based phenotyping of plants has risen in importance in recent years. Whilst much software has been written to aid phenotyping using image analysis, to date the vast majority has been only semi-automat...

    Authors: Michael P. Pound, Susan Fozard, Mercedes Torres Torres, Brian G. Forde and Andrew P. French

    Citation: Plant Methods 2017 13:12

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  18. Content type: Methodology

    Belowground processes play an essential role in ecosystem nutrient cycling and the global carbon budget cycle. Quantifying fine root growth is crucial to the understanding of ecosystem structure and function a...

    Authors: Awaz Mohamed, Yogan Monnier, Zhun Mao, Guillaume Lobet, Jean-Luc Maeght, Merlin Ramel and Alexia Stokes

    Citation: Plant Methods 2017 13:11

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  19. Content type: Methodology

    Chemical genetics provides a powerful alternative to conventional genetics for understanding gene function. However, its application to plants has been limited by the lack of a technology that allows detailed ...

    Authors: Thomas Burrell, Susan Fozard, Geoff H. Holroyd, Andrew P. French, Michael P. Pound, Christopher J. Bigley, C. James Taylor and Brian G. Forde

    Citation: Plant Methods 2017 13:10

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  20. Content type: Methodology

    Accurate floral staging is required to aid research into pollen and flower development, in particular male development. Pollen development is highly sensitive to stress and is critical for crop yields. Researc...

    Authors: Saoirse R. Tracy, José Fernández Gómez, Craig J. Sturrock, Zoe A. Wilson and Alison C. Ferguson

    Citation: Plant Methods 2017 13:9

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  21. Content type: Research

    The fitness of the rape leaf is closely related to its biomass and photosynthesis. The study of leaf traits is significant for improving rape leaf production and optimizing crop management. Canopy structure an...

    Authors: Xiong Xiong, Lejun Yu, Wanneng Yang, Meng Liu, Ni Jiang, Di Wu, Guoxing Chen, Lizhong Xiong, Kede Liu and Qian Liu

    Citation: Plant Methods 2017 13:7

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  22. Content type: Research

    Due to the complex process of designing and manufacturing new plant tissue culture vessels through conventional means there have been limited efforts to innovate improved designs. Further, development and avai...

    Authors: Mukund R. Shukla, Amritpal S. Singh, Kevin Piunno, Praveen K. Saxena and A. Maxwell P. Jones

    Citation: Plant Methods 2017 13:6

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  23. Content type: Methodology

    Biological materials have a complex, hierarchical structure, with vital structural features present at all size scales, from the nanoscale to the macroscale. A method that can connect information at multiple l...

    Authors: Patrik Ahvenainen, Patrick G. Dixon, Aki Kallonen, Heikki Suhonen, Lorna J. Gibson and Kirsi Svedström

    Citation: Plant Methods 2017 13:5

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  24. Content type: Methodology

    The analysis of circadian leaf movement rhythms is a simple yet effective method to study effects of treatments or gene mutations on the circadian clock of plants. Currently, leaf movements are analysed using ...

    Authors: Lucas Wagner, Christoph Schmal, Dorothee Staiger and Selahattin Danisman

    Citation: Plant Methods 2017 13:2

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  25. Content type: Research

    Modern agriculture uses hyperspectral cameras to obtain hundreds of reflectance data measured at discrete narrow bands to cover the whole visible light spectrum and part of the infrared and ultraviolet light s...

    Authors: Osval A. Montesinos-López, Abelardo Montesinos-López, José Crossa, Gustavo de los Campos, Gregorio Alvarado, Mondal Suchismita, Jessica Rutkoski, Lorena González-Pérez and Juan Burgueño

    Citation: Plant Methods 2017 13:4

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  26. Content type: Methodology

    Virus-induced gene silencing (VIGS) has become a powerful tool for post-genomic technology in plant species. This is important, especially in select plants, such as the pepper plant, that are recalcitrant to Agro...

    Authors: Jihyun Kim, Minkyu Park, Eun Soo Jeong, Je Min Lee and Doil Choi

    Citation: Plant Methods 2017 13:3

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  27. Content type: Methodology

    The interaction of Pseudomonas syringae with Arabidopsis is one of the most commonly used systems to study various bacterial—host interrelationships. Currently, most studies are based on the growth quantification...

    Authors: Annegret Ross and Imre E. Somssich

    Citation: Plant Methods 2016 12:48

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  28. Content type: Methodology

    Non-invasive and high-throughput monitoring of drought in plants from its initiation to visible symptoms is essential to quest drought tolerant varieties. Among the existing methods, chlorophyll a fluorescence (C...

    Authors: Kumud B. Mishra, Anamika Mishra, Kateřina Novotná, Barbora Rapantová, Petra Hodaňová, Otmar Urban and Karel Klem

    Citation: Plant Methods 2016 12:46

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  29. Content type: Methodology

    The elucidation of complex biological systems requires integration of multiple molecular parameters. Accordingly, high throughput methods like transcriptomics, proteomics, metabolomics and lipidomics have emer...

    Authors: Mohamed A. Salem, Jessica Jüppner, Krzysztof Bajdzienko and Patrick Giavalisco

    Citation: Plant Methods 2016 12:45

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  30. Content type: Methodology

    Plant phenotypic data shrouds a wealth of information which, when accurately analysed and linked to other data types, brings to light the knowledge about the mechanisms of life. As phenotyping is a field of re...

    Authors: Hanna Ćwiek-Kupczyńska, Thomas Altmann, Daniel Arend, Elizabeth Arnaud, Dijun Chen, Guillaume Cornut, Fabio Fiorani, Wojciech Frohmberg, Astrid Junker, Christian Klukas, Matthias Lange, Cezary Mazurek, Anahita Nafissi, Pascal Neveu, Jan van Oeveren, Cyril Pommier…

    Citation: Plant Methods 2016 12:44

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  31. Content type: Methodology

    The complexity of RNA regulation is one of the current frontiers in animal and plant molecular biology research. RNA-binding proteins (RBPs) are characteristically involved in post-transcriptional gene regulat...

    Authors: Zhicheng Zhang, Kurt Boonen, Piero Ferrari, Liliane Schoofs, Ewald Janssens, Vera van Noort, Filip Rolland and Koen Geuten

    Citation: Plant Methods 2016 12:42

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  32. Content type: Research

    Transcription factors (TFs) coordinate precise gene expression patterns that give rise to distinct phenotypic outputs. The identification of genes and transcriptional networks regulated by a TF often requires ...

    Authors: Donna M. Bond, Nick W. Albert, Robyn H. Lee, Gareth B. Gillard, Chris M. Brown, Roger P. Hellens and Richard C. Macknight

    Citation: Plant Methods 2016 12:41

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  33. Content type: Research

    Phenotyping of genotype-by-environment interactions in the root-zone is of major importance for crop improvement as the spatial distribution of a plant’s root system is crucial for a plant to access water and ...

    Authors: Chantal Le Marié, Norbert Kirchgessner, Patrick Flütsch, Johannes Pfeifer, Achim Walter and Andreas Hund

    Citation: Plant Methods 2016 12:40

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  34. Content type: Methodology

    Despite advances in other model organisms, there are currently no techniques to explore cell-to-cell variation and sub-cellular localization of RNA molecules at the single-cell level in plants.

    Authors: Susan Duncan, Tjelvar S. G. Olsson, Matthew Hartley, Caroline Dean and Stefanie Rosa

    Citation: Plant Methods 2016 12:13

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  35. Content type: Methodology

    The double digest restriction-site associated DNA sequencing technology (ddRAD-seq) is a reduced representation sequencing technology by sampling genome-wide enzyme loci developed on the basis of next-generati...

    Authors: Guo-Qian Yang, Yun-Mei Chen, Jin-Peng Wang, Cen Guo, Lei Zhao, Xiao-Yan Wang, Ying Guo, Li Li, De-Zhu Li and Zhen-Hua Guo

    Citation: Plant Methods 2016 12:39

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  36. Content type: Methodology

    The plant plasma membrane is a key battleground in the war between plants and their pathogens. Plants detect the presence of pathogens at the plasma membrane using sensor proteins, many of which are targeted t...

    Authors: Patrick C. Boyle, Simon Schwizer, Sarah R. Hind, Christine M. Kraus, Susana De la Torre Diaz, Bin He and Gregory B. Martin

    Citation: Plant Methods 2016 12:38

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  37. Content type: Methodology

    Rust fungi are obligate pathogens with multiple life stages often including different spore types and multiple plant hosts. While individual rust pathogens are often associated with specific plants, a wide ran...

    Authors: Mitchell A. Ellison, Michael B. McMahon, Morris R. Bonde, Cristi L. Palmer and Douglas G. Luster

    Citation: Plant Methods 2016 12:37

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  38. Content type: Methodology

    Noise (errors) in scientific data is endemic and may have a detrimental effect on statistical analyses and experimental results. The effects of noisy data have been assessed in genome-wide association studies ...

    Authors: Filippo Biscarini, Nelson Nazzicari, Chiara Broccanello, Piergiorgio Stevanato and Simone Marini

    Citation: Plant Methods 2016 12:36

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  39. Content type: Methodology

    Low cost unmanned aerial systems (UAS) have great potential for rapid proximal measurements of plants in agriculture. In the context of plant breeding and genetics, current approaches for phenotyping a large n...

    Authors: Atena Haghighattalab, Lorena González Pérez, Suchismita Mondal, Daljit Singh, Dale Schinstock, Jessica Rutkoski, Ivan Ortiz-Monasterio, Ravi Prakash Singh, Douglas Goodin and Jesse Poland

    Citation: Plant Methods 2016 12:35

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  40. Content type: Methodology

    Genetic studies and breeding of agricultural crops frequently involve phenotypic characterization of large collections of genotypes grown in field conditions. These evaluations are typically based on visual ob...

    Authors: Peter Lootens, Tom Ruttink, Antje Rohde, Didier Combes, Philippe Barre and Isabel Roldán-Ruiz

    Citation: Plant Methods 2016 12:32

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  41. Content type: Methodology

    In order to maintain high yields while saving water and preserving non-renewable resources and thus limiting the use of chemical fertilizer, it is crucial to select plants with more efficient root systems. Thi...

    Authors: Christian Jeudy, Marielle Adrian, Christophe Baussard, Céline Bernard, Eric Bernaud, Virginie Bourion, Hughes Busset, Llorenç Cabrera-Bosquet, Frédéric Cointault, Simeng Han, Mickael Lamboeuf, Delphine Moreau, Barbara Pivato, Marion Prudent, Sophie Trouvelot, Hoai Nam Truong…

    Citation: Plant Methods 2016 12:31

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