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  1. Cellular components are controlled by genetic and physiological factors that define their shape and size. However, quantitively capturing the morphological characteristics and movement of cellular organelles f...

    Authors: Arpan Kumar Basak, Mohamadreza Mirzaei, Kazimierz Strzałka and Kenji Yamada
    Citation: Plant Methods 2021 17:109
  2. The need for rapid in-field measurement of key traits contributing to yield over many thousands of genotypes is a major roadblock in crop breeding. Recently, leaf hyperspectral reflectance data has been used t...

    Authors: Robert T. Furbank, Viridiana Silva-Perez, John R. Evans, Anthony G. Condon, Gonzalo M. Estavillo, Wennan He, Saul Newman, Richard Poiré, Ashley Hall and Zhen He
    Citation: Plant Methods 2021 17:108
  3. The characteristics of light source have an important influence on the measurement performance of canopy reflectance spectrometer. The size of the effective irradiation area and the uniformity of the light int...

    Authors: Hongfeng Yu, Yongqian Ding, Huanliang Xu, Xueni Wu and Xianglin Dou
    Citation: Plant Methods 2021 17:107
  4. The Agrobacterium-mediated transient transformation, which proved effective in diverse plant species, has been widely applied for high-throughput gene function studies due to its simplicity, rapidity, and high ef...

    Authors: Yongpeng Li, Tiantian Chen, Wei Wang, Hang Liu, Xin Yan, Kuanyu Wu-Zhang, Wei Qin, Lihui Xie, Yaojie Zhang, Bowen Peng, Xinghao Yao, Chen Wang, Sadaf-Ilyas Kayani, Xueqing Fu, Ling Li and Kexuan Tang
    Citation: Plant Methods 2021 17:106
  5. Zinc (Zn) is an important nutrient for human beings, which is also an essential micronutrient for crop growth. This study investigated the role of Zn in coordinating the mineral elements absorption in modern w...

    Authors: Xiaolong Guo, Xiangyu Ma, Jialiang Zhang, Jinghuan Zhu, Tian Lu, Qifei Wang, Xiaoming Wang, Wei Hua and Shengbao Xu
    Citation: Plant Methods 2021 17:105
  6. Litchi chinensis Sonn. is an economically important fruit tree in tropical and subtropical regions. However, litchi functional genomics is severely hindered due to its recalcitrance to regeneration and stable tra...

    Authors: Yaqi Qin, Dan Wang, Jiaxin Fu, Zhike Zhang, Yonghua Qin, Guibing Hu and Jietang Zhao
    Citation: Plant Methods 2021 17:103
  7. Since the beginning of the use of reporter proteins for expression analysis, a variety of approaches have been developed and proposed; both qualitative and quantitative. The lack of simple methods for direct o...

    Authors: Alexander A. Tyurin, Aleksandra V. Suhorukova, Igor V. Deineko, Olga S. Pavlenko, Viktoriia A. Fridman and Irina V. Goldenkova-Pavlova
    Citation: Plant Methods 2021 17:102
  8. Flexural three-point bending tests are useful for characterizing the mechanical properties of plant stems. These tests can be performed with minimal sample preparation, thus allowing tests to be performed rela...

    Authors: Nathanael Martin-Nelson, Brandon Sutherland, Michael Yancey, Chung Shan Liao, Christopher J. Stubbs and Douglas D. Cook
    Citation: Plant Methods 2021 17:101
  9. Transgenic plant suspension cells show economic potential for the production of valuable bioproducts. The sugar starvation-inducible rice αAmy3 promoter, together with its signal peptide, is widely applied to pro...

    Authors: Desyanti Saulina Sinaga, Shin-Lon Ho, Chung-An Lu, Su-May Yu and Li-Fen Huang
    Citation: Plant Methods 2021 17:99
  10. Apricot is cultivated worldwide because of its high nutritive content and strong adaptability. Its flesh is delicious and has a unique and pleasant aroma. Apricot kernel is also consumed as nuts. The genome of...

    Authors: Chen Chen, Huimin Liu, Ningning Gou, Mengzhen Huang, Wanyu Xu, Xuchun Zhu, Mingyu Yin, Haikun Bai, Lin Wang and Ta-na Wuyun
    Citation: Plant Methods 2021 17:98
  11. Detailed datasets containing root system and its architecture in soil are required to improve understanding of resource capture by roots. However, most of the root study methods have paid little attention to m...

    Authors: Takuya Koyama, Shun Murakami, Toshihiko Karasawa, Masato Ejiri and Katsuhiro Shiono
    Citation: Plant Methods 2021 17:97
  12. Fractional vegetation cover (FVC) is an important basic parameter for the quantitative monitoring of the alpine grassland ecosystem on the Qinghai-Tibetan Plateau. Based on unmanned aerial vehicle (UAV) acquis...

    Authors: Xingchen Lin, Jianjun Chen, Peiqing Lou, Shuhua Yi, Yu Qin, Haotian You and Xiaowen Han
    Citation: Plant Methods 2021 17:96
  13. Ploidy manipulation is effective in seedless loquat breeding, in which flesh color is a key agronomic and economic trait. Few techniques are currently available for detecting the genotypes of polyploids in pla...

    Authors: Haiyan Wang, Jiangbo Dang, Di Wu, Zhongyi Xie, Shuang Yan, Jingnan Luo, Qigao Guo and Guolu Liang
    Citation: Plant Methods 2021 17:93
  14. Translation is a tightly regulated process, controlling the rate of protein synthesis in cells. Ribosome sequencing (Ribo-Seq) is a recently developed tool for studying actively translated mRNA and can thus di...

    Authors: Amir Mahboubi, Nicolas Delhomme, Sara Häggström and Johannes Hanson
    Citation: Plant Methods 2021 17:92
  15. Maize cobs are an important component of crop yield that exhibit a high diversity in size, shape and color in native landraces and modern varieties. Various phenotyping approaches were developed to measure mai...

    Authors: Lydia Kienbaum, Miguel Correa Abondano, Raul Blas and Karl Schmid
    Citation: Plant Methods 2021 17:91
  16. Estimation of nitrate nitrogen (NO3–N) content in petioles is one of the key approaches for monitoring nitrogen (N) nutrition in crops. Rapid, non-destructive, and accurate evaluation of NO3–N contents in cotto...

    Authors: Zhiqiang Dong, Yang Liu, Baoxia Ci, Ming Wen, Minghua Li, Xi Lu, Xiaokang Feng, Shuai Wen and Fuyu Ma
    Citation: Plant Methods 2021 17:90
  17. Since the introduction of studies on maize silage digestibility at the end of the nineteenth century, protocols to estimate dry matter digestibility have not stopped evolving. Since the early 1980s, the protoc...

    Authors: P.-L. Lopez-Marnet, S. Guillaume, M.-P. Jacquemot, M. Reymond and V. Méchin
    Citation: Plant Methods 2021 17:89
  18. Rice is one of the most important grain crops worldwide. The accurate and dynamic monitoring of Leaf Area Index (LAI) provides important information to evaluate rice growth and production.

    Authors: Yan Gong, Kaili Yang, Zhiheng Lin, Shenghui Fang, Xianting Wu, Renshan Zhu and Yi Peng
    Citation: Plant Methods 2021 17:88
  19. Hyperaccumulation of trace elements is a rare trait among plants which is being investigated to advance our understanding of the regulation of metal accumulation and applications in phytotechnologies. Noccaea cae...

    Authors: Lucas van der Zee, Amelia Corzo Remigio, Lachlan W. Casey, Imam Purwadi, Jitpanu Yamjabok, Antony van der Ent, Gert Kootstra and Mark G. M. Aarts
    Citation: Plant Methods 2021 17:86
  20. Heterosis has been widely used in maize breeding. However, we know little about the heterotic quantitative trait loci and their roles in genomic prediction. In this study, we sought to identify heterotic quant...

    Authors: Tifu Zhang, Lu Jiang, Long Ruan, Yiliang Qian, Shuaiqiang Liang, Feng Lin, Haiyan Lu, Huixue Dai and Han Zhao
    Citation: Plant Methods 2021 17:85
  21. Plant protoplasts constitute unique single-cell systems that can be subjected to genomic, proteomic, and metabolomic analysis. An effective and sustainable method for preparing protoplasts from tea plants has ...

    Authors: Xue-feng Xu, Hai-yan Zhu, Yin-feng Ren, Can Feng, Zhi-hao Ye, Hui-mei Cai, Xiao-chun Wan and Chuan-yi Peng
    Citation: Plant Methods 2021 17:84
  22. DNA–protein interactions are essential for several molecular and cellular mechanisms, such as transcription, transcriptional regulation, DNA modifications, among others. For many decades scientists tried to un...

    Authors: Ricardo André Campos Ferraz, Ana Lúcia Gonçalves Lopes, Jessy Ariana Faria da Silva, Diana Filipa Viana Moreira, Maria João Nogueira Ferreira and Sílvia Vieira de Almeida Coimbra
    Citation: Plant Methods 2021 17:82
  23. Drought is a major consequence of global heating that has negative impacts on agriculture. Potato is a drought-sensitive crop; tuber growth and dry matter content may both be impacted. Moreover, water deficit ...

    Authors: M. Musse, G. Hajjar, N. Ali, B. Billiot, G. Joly, J. Pépin, S. Quellec, S. Challois, F. Mariette, M. Cambert, C. Fontaine, C. Ngo-Dinh, F. Jamois, A. Barbary, P. Leconte, C. Deleu…
    Citation: Plant Methods 2021 17:81
  24. The parasitic plant Orobanche cumana is one of the most important threats to sunflower crops in Europe. Resistant sunflower varieties have been developed, but new O. cumana races have evolved and have overcome in...

    Authors: A. Le Ru, G. Ibarcq, M.- C. Boniface, A. Baussart, S. Muños and M. Chabaud
    Citation: Plant Methods 2021 17:80
  25. Sugarcane (Saccharum officinarum L.) is an economically important crop with stalks as the harvest organs. Improvement in stalk quality is deemed a promising strategy for enhancing sugarcane production. However, t...

    Authors: Maoyao Wang, Xinru Li, Yinjuan Shen, Muhammad Adnan, Le Mao, Pan Lu, Qian Hu, Fuhong Jiang, Muhammad Tahir Khan, Zuhu Deng, Baoshan Chen, Jiangfeng Huang and Muqing Zhang
    Citation: Plant Methods 2021 17:76
  26. Jujube is a typical fruit tree species from China. ‘Muzao’, a cracking-susceptible cultivar, and ‘Linhuang No. 1’, a cracking-resistant cultivar, were selected in a previous study as contrasting research mater...

    Authors: Na Li, Yuqin Song, Jie Li, Ruijie Hao, Xinxin Feng and Liulin Li
    Citation: Plant Methods 2021 17:75
  27. Cassava is an important crop for food security and industry in the least-developed and developing countries. The completion of the cassava genome sequence and identification of large numbers of candidate genes...

    Authors: Decai Tuo, Peng Zhou, Pu Yan, Hongguang Cui, Yang Liu, He Wang, Xiukun Yang, Wenbin Liao, Di Sun, Xiaoying Li and Wentao Shen
    Citation: Plant Methods 2021 17:74
  28. Agrobacterium-mediated genetic transformation is a widely used and efficient technique for gene functional research in crop breeding and plant biology. While in some plant species, including soybean, genetic tran...

    Authors: Yuanyuan Cheng, Xiaoli Wang, Li Cao, Jing Ji, Tengfei Liu and Kaixuan Duan
    Citation: Plant Methods 2021 17:73
  29. Surface roughness has a significant effect on leaf wettability. Consequently, it influences the efficiency and effectiveness of pesticide application. Therefore, roughness measurement of leaf surface offers su...

    Authors: Zeqing Zhang, Fei Liu, Zhenjiang Zhou, Yong He and Hui Fang
    Citation: Plant Methods 2021 17:72
  30. CRISPR/Cas9 is a rapidly developing genome editing technology in various biological systems due to its efficiency, portability, simplicity and versatility. This editing technology has been successfully applied...

    Authors: Wang Wang, Jiangmiao Liu, Hai Wang, Tong Li and Huien Zhao
    Citation: Plant Methods 2021 17:71
  31. Stipagrostis pennata (Trin.) De Winter is an important species for fixing sand in shifting and semi-fixed sandy lands, for grazing, and potentially as a source of lignocellulose fibres for pulp and paper industry...

    Authors: Masoumeh Asadi-Aghbolaghi, Beata Dedicova, Sonali Sachi Ranade, Kim-Cuong Le, Farzad Sharifzadeh, Mansoor Omidi and Ulrika Egertsdotter
    Citation: Plant Methods 2021 17:70
  32. Obtaining instantaneous gas exchanges data is fundamental to gain information on photosynthesis. Leaf level data are reliable, but their scaling up to canopy scale is difficult as they are acquired in standard...

    Authors: Nicole Salvatori, Alberti Giorgio, Onno Muller, Uwe Rascher and Alessandro Peressotti
    Citation: Plant Methods 2021 17:69
  33. Accurate and efficient measurement of the diameter at breast height (DBH) of individual trees is essential for forest inventories, ecological management, and carbon budget estimation. However, traditional diam...

    Authors: Chuangye Song, Bin Yang, Lin Zhang and Dongxiu Wu
    Citation: Plant Methods 2021 17:67
  34. Protocols for Agrobacterium rhizogenes-mediated hairy root transformation of the model legume Lotus japonicus have been established previously. However, little efforts were made in the past to quantify and improv...

    Authors: Yan Wang, Feng Yang, Peng-Fei Zhu, Asaf Khan, Zhi-Ping Xie and Christian Staehelin
    Citation: Plant Methods 2021 17:66
  35. The study of plant phenotype by deep learning has received increased interest in recent years, which impressive progress has been made in the fields of plant breeding. Deep learning extremely relies on a large...

    Authors: Ruisong Zhang, Ye Tian, Junmei Zhang, Silan Dai, Xiaogai Hou, Jue Wang and Qi Guo
    Citation: Plant Methods 2021 17:65
  36. To enforce timber import laws and perform timber species identification, the identity of the botanical species must be well-defined. Since the Sapotaceae family is known as a taxonomically challenging family, ...

    Authors: V. Deklerck, E. Price, S. Vanden Abeele, K. Lievens, E. Espinoza and H. Beeckman
    Citation: Plant Methods 2021 17:64
  37. Astragalus mongholicus Bunge is an important medicinal plant used in traditional Chinese medicine. It is rich in isoflavonoids and triterpenoid saponins. Although these active constituents of A. mongholicus have ...

    Authors: Minzhen Yin, Shanshan Chu, Tingyu Shan, Liangping Zha and Huasheng Peng
    Citation: Plant Methods 2021 17:61
  38. The fraction of intercepted photosynthetically active radiation (fPARi) is typically described with a non-linear function of leaf area index (LAI) and k, the light extinction coefficient. The parameter k is used ...

    Authors: Josefina Lacasa, Trevor J. Hefley, María E. Otegui and Ignacio A. Ciampitti
    Citation: Plant Methods 2021 17:60

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