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  1. In recent years, there has been an increase of interest in plant behaviour as represented by growth-driven responses. These are generally classified into nastic (internally driven) and tropic (environmentally ...

    Authors: Gabriella E. C. Gall, Talmo D. Pereira, Alex Jordan and Yasmine Meroz
    Citation: Plant Methods 2022 18:21
  2. Some plastid-derived metabolites can control nuclear gene expression, chloroplast biogenesis, and chlorophyll biosynthesis. For example, norflurazon (NFZ) induced inhibition of carotenoid biosynthesis in leave...

    Authors: N. Dhami, B. J. Pogson, D. T. Tissue and C. I. Cazzonelli
    Citation: Plant Methods 2022 18:18
  3. Layered double hydroxide lactate nanosheets (LDH-lactate-NS) are powerful carriers for delivering macro-molecules into intact plant cells. In the past few years, some studies have been carried out on DNA/RNA t...

    Authors: Hongyang Wu, He Zhang, Xinyu Li, Yu Zhang, Jiankun Wang, Qiang Wang and Yinglang Wan
    Citation: Plant Methods 2022 18:17
  4. RNA live-cell imaging systems have been used to visualize subcellular mRNA distribution in living cells. The RNA-binding protein (RBP)-based RNA imaging system exploits specific RBP and the corresponding RNA r...

    Authors: Nien-Chen Huang, Kai-Ren Luo and Tien-Shin Yu
    Citation: Plant Methods 2022 18:15
  5. Developing a systematic phenotypic data analysis pipeline, creating enhanced visualizations, and interpreting the results is crucial to extract meaningful insights from data in making better breeding decisions...

    Authors: Waseem Hussain, Mahender Anumalla, Margaret Catolos, Apurva Khanna, Ma. Teresa Sta. Cruz, Joie Ramos and Sankalp Bhosale
    Citation: Plant Methods 2022 18:14
  6. Precision agriculture techniques are widely used to optimize fertilizer and soil applications. Furthermore, these techniques could also be combined with new statistical tools to assist in phenotyping in breedi...

    Authors: Dthenifer Cordeiro Santana, Marcos Paulo de Oliveira Cunha, Regimar Garcia dos Santos, Mayara Fávero Cotrim, Larissa Pereira Ribeiro Teodoro, Carlos Antonio da Silva Junior, Fabio Henrique Rojo Baio and Paulo Eduardo Teodoro
    Citation: Plant Methods 2022 18:13
  7. Rosette leaf trichomes of Arabidopsis thaliana have been broadly used to study cell development, cell differentiation and, more recently, cell wall biogenesis. However, trichome-specific biochemical or -omics ana...

    Authors: Jan W. Huebbers, Kim Büttgen, Franz Leissing, Melissa Mantz, Markus Pauly, Pitter F. Huesgen and Ralph Panstruga
    Citation: Plant Methods 2022 18:12
  8. Plant plasma membrane transporters play essential roles during the translocation of vectorized agrochemicals. Therefore, transporters associated with phloem loading of vectorized agrochemicals have drawn incre...

    Authors: Yongxin Xiao, Jinying Zhang, Yiting Li, Tom Hsiang, Xingping Zhang, Yongxing Zhu, Xiaoying Du, Junliang Yin and Junkai Li
    Citation: Plant Methods 2022 18:11
  9. Dendrobium nobile Lindl. is an important pharmacopeial plant with medicinal and ornamental value. This study sought to provide a technical means for the large-scale production of total alkaloid in D. nobile. Seed...

    Authors: Benhou Zhang, Zhitao Niu, Chao Li, Zhenyu Hou, Qingyun Xue, Wei Liu and Xiaoyu Ding
    Citation: Plant Methods 2022 18:10
  10. Rice is a major staple food crop for more than half the world’s population. As the global population is expected to reach 9.7 billion by 2050, increasing the production of high-quality rice is needed to meet t...

    Authors: Chaoxin Wang, Doina Caragea, Nisarga Kodadinne Narayana, Nathan T. Hein, Raju Bheemanahalli, Impa M. Somayanda and S. V. Krishna Jagadish
    Citation: Plant Methods 2022 18:9
  11. Leaf hairiness (pubescence) is an important plant phenotype which regulates leaf transpiration, affects sunlight penetration, and provides increased resistance or susceptibility against certain insects. Cotton...

    Authors: Vivien Rolland, Moshiur R. Farazi, Warren C. Conaty, Deon Cameron, Shiming Liu, Lars Petersson and Warwick N. Stiller
    Citation: Plant Methods 2022 18:8
  12. Ralstonia solanacearum, one of the most devastating bacterial plant pathogens, is the causal agent of bacterial wilt. Recently, several studies on resistance to bacterial wilt have been conducted using the Arabid...

    Authors: Cuihong Xu, Lingkun Zhong, Zeming Huang, Chenying Li, Jiazhang Lian, Xuefang Zheng and Yan Liang
    Citation: Plant Methods 2022 18:7
  13. The incorporation of root traits into elite germplasm is typically a slow process. Thus, innovative approaches are required to accelerate research and pre-breeding programs targeting root traits to improve yie...

    Authors: Charlotte Rambla, Sarah Van Der Meer, Kai P. Voss-Fels, Manar Makhoul, Christian Obermeier, Rod Snowdon, Eric S. Ober, Michelle Watt, Samir Alahmad and Lee T. Hickey
    Citation: Plant Methods 2022 18:2
  14. Lupins are promising protein crops with an increasing amount of genomic and transcriptomic resources. The new resources facilitate the in silico identification of candidate genes controlling important agronomi...

    Authors: Davide Mancinotti, Maria Cecilia Rodriguez, Karen Michiko Frick, Bjørn Dueholm, Ditte Goldschmidt Jepsen, Niels Agerbirk and Fernando Geu-Flores
    Citation: Plant Methods 2021 17:131
  15. With increasing joint research cooperation on national and international levels, there is a high need for harmonized and reproducible cultivation conditions and experimental protocols in order to ensure the be...

    Authors: Paul Grünhofer, Yayu Guo, Ruili Li, Jinxing Lin and Lukas Schreiber
    Citation: Plant Methods 2021 17:129
  16. The leaf epidermis functions to prevent the loss of water and reduce gas exchange. As an interface between the plant and its external environment, it helps prevent damage, making it an attractive system for st...

    Authors: Jieping Li, Xinlei Feng and Jinjin Xie
    Citation: Plant Methods 2021 17:128
  17. 3D imaging, such as X-ray CT and MRI, has been widely deployed to study plant root structures. Many computational tools exist to extract coarse-grained features from 3D root images, such as total volume, root ...

    Authors: Dan Zeng, Mao Li, Ni Jiang, Yiwen Ju, Hannah Schreiber, Erin Chambers, David Letscher, Tao Ju and Christopher N. Topp
    Citation: Plant Methods 2021 17:127
  18. The bacterial leaf pathogen Pseudomonas syringae pv tomato (Pst) is the most popular model pathogen for plant pathology research. Previous methods to study the plant-Pst interactions rely on destructive quantific...

    Authors: Leonardo Furci, David Pascual-Pardo and Jurriaan Ton
    Citation: Plant Methods 2021 17:126
  19. The root system architecture (RSA) of alfalfa (Medicago sativa L.) affects biomass production by influencing water and nutrient uptake, including nitrogen fixation. Further, roots are important for storing carboh...

    Authors: Bruna Bucciarelli, Zhanyou Xu, Samadangla Ao, Yuanyuan Cao, Maria J. Monteros, Christopher N. Topp and Deborah A. Samac
    Citation: Plant Methods 2021 17:125
  20. Ribo-seq has revolutionized the study of genome-wide mRNA translation. High-quality Ribo-seq data display strong 3-nucleotide (nt) periodicity, which corresponds to translating ribosomes deciphering three nts ...

    Authors: Hsin-Yen Larry Wu and Polly Yingshan Hsu
    Citation: Plant Methods 2021 17:124
  21. Breakthrough imaging technologies may challenge the plant phenotyping bottleneck regarding marker-assisted breeding and genetic mapping. In this context, X-Ray CT (computed tomography) technology can accuratel...

    Authors: Monica Herrero-Huerta, Valerian Meline, Anjali S. Iyer-Pascuzzi, Augusto M. Souza, Mitchell R. Tuinstra and Yang Yang
    Citation: Plant Methods 2021 17:123
  22. Blackleg disease, caused by the fungal pathogen Leptosphaeria maculans, is a serious threat to canola (Brassica napus) production worldwide. Quantitative resistance to this disease is a highly desirable trait but...

    Authors: Wendelin Schnippenkoetter, Mohammad Hoque, Rebecca Maher, Angela Van de Wouw, Phillip Hands, Vivien Rolland, Luke Barrett and Susan Sprague
    Citation: Plant Methods 2021 17:122
  23. The rice gall midge (RGM, Orseolia oryzae, Wood-Mason), an important stem-feeding pest worldwide, has caused serious production losses over the past decades. Rice production practices indicate that the most relia...

    Authors: Ling Cheng, Fugang Huang, Zhe Jiang, Baiyi Lu, Xiaohui Zhong and Yongfu Qiu
    Citation: Plant Methods 2021 17:121
  24. Low temperature freezing stress has adverse effects on wheat seedling growth and final yield. The traditional method to evaluate the wheat injury caused by the freezing stress is by visual observations, which ...

    Authors: Xiuqing Fu, Yang Bai, Jing Zhou, Hongwen Zhang and Jieyu Xian
    Citation: Plant Methods 2021 17:120
  25. Due to the high cost of data collection for magnetization detection of media, the sample size is limited, it is not suitable to use deep learning method to predict its change trend. The prediction of physical ...

    Authors: Jing Nie, Nianyi Wang, Jingbin Li, Kang Wang and Hongkun Wang
    Citation: Plant Methods 2021 17:119
  26. Realizing imaging detection of water and nitrogen content in different regions of plant leaves in-site and real-time can provide an efficient new technology for determining crop drought resistance and nutrient...

    Authors: Ning Zhang, Peng-cheng Li, Hubin Liu, Tian-cheng Huang, Han Liu, Yu Kong, Zhi-cheng Dong, Yu-hui Yuan, Long-lian Zhao and Jun-hui Li
    Citation: Plant Methods 2021 17:117
  27. Macrophytes are key players in aquatic ecosystems diversity, but knowledge on variability of their functional traits, among and within species, is still limited. Remote sensing is a high-throughput, feasible o...

    Authors: Paolo Villa, Rossano Bolpagni, Monica Pinardi and Viktor R. Tóth
    Citation: Plant Methods 2021 17:115
  28. Multiplex CRISPR-Cas9-based genome editing is an efficient method for targeted disruption of gene function in plants. Use of CRISPR-Cas9 has increased rapidly in recent years and is becoming a routine method f...

    Authors: Robertas Ursache, Satoshi Fujita, Valérie Dénervaud Tendon and Niko Geldner
    Citation: Plant Methods 2021 17:111
  29. Grapevine, as an essential fruit crop with high economic values, has been the focus of molecular studies in diverse areas. Two challenges exist in the grapevine research field: (i) the lack of a rapid, user-fr...

    Authors: Yashu Song, Robert H. Hanner and Baozhong Meng
    Citation: Plant Methods 2021 17:110
  30. Cellular components are controlled by genetic and physiological factors that define their shape and size. However, quantitively capturing the morphological characteristics and movement of cellular organelles f...

    Authors: Arpan Kumar Basak, Mohamadreza Mirzaei, Kazimierz Strzałka and Kenji Yamada
    Citation: Plant Methods 2021 17:109
  31. The need for rapid in-field measurement of key traits contributing to yield over many thousands of genotypes is a major roadblock in crop breeding. Recently, leaf hyperspectral reflectance data has been used t...

    Authors: Robert T. Furbank, Viridiana Silva-Perez, John R. Evans, Anthony G. Condon, Gonzalo M. Estavillo, Wennan He, Saul Newman, Richard Poiré, Ashley Hall and Zhen He
    Citation: Plant Methods 2021 17:108
  32. The characteristics of light source have an important influence on the measurement performance of canopy reflectance spectrometer. The size of the effective irradiation area and the uniformity of the light int...

    Authors: Hongfeng Yu, Yongqian Ding, Huanliang Xu, Xueni Wu and Xianglin Dou
    Citation: Plant Methods 2021 17:107
  33. The Agrobacterium-mediated transient transformation, which proved effective in diverse plant species, has been widely applied for high-throughput gene function studies due to its simplicity, rapidity, and high ef...

    Authors: Yongpeng Li, Tiantian Chen, Wei Wang, Hang Liu, Xin Yan, Kuanyu Wu-Zhang, Wei Qin, Lihui Xie, Yaojie Zhang, Bowen Peng, Xinghao Yao, Chen Wang, Sadaf-Ilyas Kayani, Xueqing Fu, Ling Li and Kexuan Tang
    Citation: Plant Methods 2021 17:106
  34. Zinc (Zn) is an important nutrient for human beings, which is also an essential micronutrient for crop growth. This study investigated the role of Zn in coordinating the mineral elements absorption in modern w...

    Authors: Xiaolong Guo, Xiangyu Ma, Jialiang Zhang, Jinghuan Zhu, Tian Lu, Qifei Wang, Xiaoming Wang, Wei Hua and Shengbao Xu
    Citation: Plant Methods 2021 17:105
  35. Litchi chinensis Sonn. is an economically important fruit tree in tropical and subtropical regions. However, litchi functional genomics is severely hindered due to its recalcitrance to regeneration and stable tra...

    Authors: Yaqi Qin, Dan Wang, Jiaxin Fu, Zhike Zhang, Yonghua Qin, Guibing Hu and Jietang Zhao
    Citation: Plant Methods 2021 17:103

    The Correction to this article has been published in Plant Methods 2022 18:140

  36. Since the beginning of the use of reporter proteins for expression analysis, a variety of approaches have been developed and proposed; both qualitative and quantitative. The lack of simple methods for direct o...

    Authors: Alexander A. Tyurin, Aleksandra V. Suhorukova, Igor V. Deineko, Olga S. Pavlenko, Viktoriia A. Fridman and Irina V. Goldenkova-Pavlova
    Citation: Plant Methods 2021 17:102

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