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  1. Environmental stress due to climate or pathogens is a major threat to modern agriculture. Plant genetic resistance to these stresses is one way to develop more resilient crops, but accurately quantifying plant...

    Authors: Changye Yang, Sriram Baireddy, Valérian Méline, Enyu Cai, Denise Caldwell, Anjali S. Iyer-Pascuzzi and Edward J. Delp
    Citation: Plant Methods 2023 19:52
  2. The advancements in unmanned aerial vehicle (UAV) technology have recently emerged as an effective, cost-efficient, and versatile solution for monitoring crop growth with high spatial and temporal precision. T...

    Authors: Yuxiang Wang, Zengling Yang, Gert Kootstra and Haris Ahmad Khan
    Citation: Plant Methods 2023 19:51

    The Correction to this article has been published in Plant Methods 2023 19:62

  3. Passion fruit is an essential commercial plant in the tropics and subtropics, which has lately seen a rise in demand for high-quality fruits and large-scale production. Generally, different species of passion ...

    Authors: Mohammad Aqa Mohammadi, Myat Hnin Wai, Hafiz Muhammad Rizwan, Abdul Qahar Qarluq, Mengjie Xu, Lulu Wang, Yan Cheng, Mohammad Aslam, Ping Zheng, Xiaomei Wang, Wenbin Zhang and Yuan Qin
    Citation: Plant Methods 2023 19:50
  4. A well-known method for evaluating plant resistance to insects is by measuring insect reproduction or oviposition. Whiteflies are vectors of economically important viral diseases and are, therefore, widely stu...

    Authors: Micha Gracianna Devi, Dan Jeric Arcega Rustia, Lize Braat, Kas Swinkels, Federico Fornaguera Espinosa, Bart M. van Marrewijk, Jochen Hemming and Lotte Caarls
    Citation: Plant Methods 2023 19:49
  5. Nitrogen (N), phosphorus (P), and potassium (K) contents are crucial quality indicators for forage in alpine natural grasslands and are closely related to plant growth and reproduction. One of the greatest cha...

    Authors: Xuanfan Zhang, Tiangang Liang, Jinlong Gao, Dongmei Zhang, Jie Liu, Qisheng Feng, Caixia Wu and Zhiwei Wang
    Citation: Plant Methods 2023 19:48
  6. Climate change due to different human activities is causing adverse environmental conditions and uncontrolled extreme weather events. These harsh conditions are directly affecting the crop areas, and consequen...

    Authors: Jimmy Sampedro-Guerrero, Vicente Vives-Peris, Aurelio Gomez-Cadenas and Carolina Clausell-Terol
    Citation: Plant Methods 2023 19:47
  7. Detecting and counting wheat spikes is essential for predicting and measuring wheat yield. However, current wheat spike detection researches often directly apply the new network structure. There are few studie...

    Authors: Jiawei Yan, Jianqing Zhao, Yucheng Cai, Suwan Wang, Xiaolei Qiu, Xia Yao, Yongchao Tian, Yan Zhu, Weixing Cao and Xiaohu Zhang
    Citation: Plant Methods 2023 19:46
  8. The Mg–Al-lactate layered double hydroxide nanosheet (LDH-NS) has shown great potential as an optimal nanocarrier for extensive use in plants. However, previous studies in plant sciences have not provided a cl...

    Authors: He Zhang, Xinyu Li, Dong Yu, Junqi Guan, Hao Ding, Hongyang Wu, Qiang Wang and Yinglang Wan
    Citation: Plant Methods 2023 19:44
  9. The current development of sensor technologies towards ever more cost-effective and powerful systems is steadily increasing the application of low-cost sensors in different horticultural sectors. In plant in v...

    Authors: Hans Bethge, Traud Winkelmann, Patrick Lüdeke and Thomas Rath
    Citation: Plant Methods 2023 19:42

    The Correction to this article has been published in Plant Methods 2023 19:133

  10. PacBio HiFi sequencing provides highly accurate long-read sequencing datasets which are of great advantage for whole genome sequencing projects. One limitation of the method is the requirement for high quality...

    Authors: Kanae Nishii, Michael Möller, Robert G. Foster, Laura L. Forrest, Nathan Kelso, Sadie Barber, Caroline Howard and Michelle L. Hart
    Citation: Plant Methods 2023 19:41
  11. The flowering period is a critical time for the growth of rape plants. Counting rape flower clusters can help farmers to predict the yield information of the corresponding rape fields. However, counting in-fie...

    Authors: Jie Li, Enguo Wang, Jiangwei Qiao, Yi Li, Li Li, Jian Yao and Guisheng Liao
    Citation: Plant Methods 2023 19:40
  12. Canopy height (CH) is an important trait for crop breeding and production. The rapid development of 3D sensing technologies shed new light on high-throughput height measurement. However, a systematic compariso...

    Authors: Jingrong Zang, Shichao Jin, Songyin Zhang, Qing Li, Yue Mu, Ziyu Li, Shaochen Li, Xiao Wang, Yanjun Su and Dong Jiang
    Citation: Plant Methods 2023 19:39
  13. The identification and enumeration of medicinal plants at high elevations is an important part of accurate yield calculations. However, the current assessment of medicinal plant reserves continues to rely on f...

    Authors: Rong Ding, Jiawei Luo, Chenghui Wang, Lianhui Yu, Jiangkai Yang, Meng Wang, Shihong Zhong and Rui Gu
    Citation: Plant Methods 2023 19:38
  14. Decades of research is available on their effects of single component surfactant on active ingredient diffusion across plant cuticular membranes, but ingredient diffusion is rarely analysed in the presence of ...

    Authors: Lakshmi Venkatesha Manyu Vittal, James Rookes, Ben Boyd and David Cahill
    Citation: Plant Methods 2023 19:37
  15. Stomata are tiny pores on the leaf surface that are central to gas exchange. Stomatal number, size and aperture are key determinants of plant transpiration and photosynthesis, and variation in these traits can...

    Authors: Phetdalaphone Pathoumthong, Zhen Zhang, Stuart J. Roy and Abdeljalil El Habti
    Citation: Plant Methods 2023 19:36
  16. Cell characteristics, including cell type, size, shape, packing, cell-to-cell-adhesion, intercellular space, and cell wall thickness, influence the physical characteristics of plant tissues. Genotypic differen...

    Authors: Nelia Nause, Facundo R. Ispizua Yamati, Marion Seidel, Anne-Katrin Mahlein and Christa M. Hoffmann
    Citation: Plant Methods 2023 19:35
  17. Insertion of Mg2+ into protoporphyrin IX (PPIX) to produce magnesium-protoporphyrin IX (Mg-PPIX) was the first step toward chlorophyll biosynthesis, which not only imparts plants green pigmentation but underpins ...

    Authors: Chenyu Zhang, Chunlei Ma, Li Zhu and Mingzhe Yao
    Citation: Plant Methods 2023 19:34
  18. Plant architecture can influence crop yield and quality. Manual extraction of architectural traits is, however, time-consuming, tedious, and error prone. The trait estimation from 3D data addresses occlusion i...

    Authors: Farah Saeed, Shangpeng Sun, Javier Rodriguez-Sanchez, John Snider, Tianming Liu and Changying Li
    Citation: Plant Methods 2023 19:33
  19. Necrotizing pathogens pose an immense economic and ecological threat to trees and forests, but the molecular analysis of these pathogens is still in its infancy because of lacking model systems. To close this ...

    Authors: Steven Dreischhoff, Ishani Shankar Das, Felix Häffner, Anna Malvine Wolf, Andrea Polle and Karl Henrik Kasper
    Citation: Plant Methods 2023 19:32
  20. Even for easy-to-transform species or genotypes, the creation of transgenic or edited plant lines remains a significant bottleneck. Thus, any technical advance that accelerates the regeneration and transformat...

    Authors: Camille Soulhat, Houssein Wehbi, Yannick Fierlej, Patrick Berquin, Thomas Girin, Pierre Hilson and Oumaya Bouchabké-Coussa
    Citation: Plant Methods 2023 19:31
  21. In plant genome editing, RNA-guided nucleases such as Cas9 from Streptococcus pyogenes (SpCas9) predominantly induce small insertions or deletions at target sites. This can be used for inactivation of protein-cod...

    Authors: Jana Ordon, Niklas Kiel, Dieter Becker, Carola Kretschmer, Paul Schulze-Lefert and Johannes Stuttmann
    Citation: Plant Methods 2023 19:30
  22. Remote sensing instruments enable high-throughput phenotyping of plant traits and stress resilience across scale. Spatial (handheld devices, towers, drones, airborne, and satellites) and temporal (continuous o...

    Authors: Christopher Y. S. Wong, Taylor Jones, Devin P. McHugh, Matthew E. Gilbert, Paul Gepts, Antonia Palkovic, Thomas N. Buckley and Troy S. Magney
    Citation: Plant Methods 2023 19:29
  23. Selection is one of the essential skills whereby breeders reduce the population size and increase the chance of success. Various selection methods with special applications have been developed. Superior genoty...

    Authors: Peyman Eynizadeh, Hamid Dehghani and Ali Dehghani
    Citation: Plant Methods 2023 19:25
  24. As one of the largest drupes in the world, the coconut has a special multilayered structure and a seed development process that is not yet fully understood. On the one hand, the special structure of the coconu...

    Authors: Yu Zhang, Qianfan Liu, Jing Chen, Chengxu Sun, Shenghuang Lin, Hongxing Cao, Zhaolin Xiao and Mengxing Huang
    Citation: Plant Methods 2023 19:24
  25. The rapidly advancing corn breeding field calls for high-throughput methods to phenotype corn kernel traits to estimate yield and to study their genetic inheritance. Most of the existing methods are reliant on...

    Authors: Samantha Gillette, Lu Yin, Penny M. A. Kianian, Wojciech P. Pawlowski and Changbin Chen
    Citation: Plant Methods 2023 19:23
  26. Root-knot nematodes (RKNs) pose a worldwide threat to agriculture of many crops including cucumber. Genetic transformation (GT) has emerged as a powerful tool for exploration of plant-RKN interactions and gene...

    Authors: Xu Zhang, Shihui Li, Xin Li, Mengyuan Song, Si Ma, Yongqiang Tian and Lihong Gao
    Citation: Plant Methods 2023 19:22
  27. Various growth systems are available for studying plant root growth and plant–microbe interactions including hydroponics and aeroponics. Although some of these systems work well with Arabidopsis thaliana and smal...

    Authors: Jingya Cai, Vijaykumar Veerappan, Kate Arildsen, Catrina Sullivan, Megan Piechowicz, Julia Frugoli and Rebecca Dickstein
    Citation: Plant Methods 2023 19:21

    The Correction to this article has been published in Plant Methods 2023 19:28

  28. Bamboo is a perennial and renewable biomass forest resource and its leaf flavonoid is an antioxidant for biological and pharmacological research. The established genetic transformation and gene editing systems...

    Authors: Huayu Sun, Sining Wang, Chenglei Zhu, Kebin Yang, Yan Liu and Zhimin Gao
    Citation: Plant Methods 2023 19:20
  29. Non-invasive reporter systems are powerful tools to query physiological and transcriptional responses in organisms. For example, fluorescent and bioluminescent reporters have revolutionized cellular and organi...

    Authors: Maria L. Sorkin, Kathleen K Markham, Stevan Zorich, Ananda Menon, Kristen N. Edgeworth, Angela Ricono, Douglas Bryant, Rebecca Bart, Dmitri A. Nusinow and Kathleen Greenham
    Citation: Plant Methods 2023 19:19
  30. Lignocellulosic biomass is a complex network of polysaccharides and lignin that requires a pretreatment step to overcome recalcitrance and optimize valorisation into biobased products. Pretreatment of biomass ...

    Authors: Edwige Audibert, Berangère Lebas, Corentin Spriet, Anouck Habrant, Brigitte Chabbert and Gabriel Paës
    Citation: Plant Methods 2023 19:16
  31. Agrobacterium tumefaciens-mediated leaf disc genetic transformation is an important way to achieve transgenics or gene editing. Ensuring stable and efficient genetic transformation is still an important problem i...

    Authors: Yufei Xia, Yuan Cao, Yongyu Ren, Aoyu Ling, Kang Du, Yun Li, Jun Yang and Xiangyang Kang
    Citation: Plant Methods 2023 19:15
  32. Herbicide resistance is one of the threats to modern agriculture and its early detection is one of the most effective components for sustainable resistance management strategies. Many techniques have been used...

    Authors: Silvia Panozzo, Silvia Farinati, Maurizio Sattin and Laura Scarabel
    Citation: Plant Methods 2023 19:14
  33. Despite the increased efficiency of sequencing technologies and the development of reduced-representation sequencing (RRS) approaches allowing high-throughput sequencing (HTS) of multiplexed samples, the per-s...

    Authors: Maxime de Ronne, Gaétan Légaré, François Belzile, Brian Boyle and Davoud Torkamaneh
    Citation: Plant Methods 2023 19:13
  34. Microscopic analysis of plant anatomy is a common procedure in biology to study structure and function that requires high-quality sections for accurate measurements. Hand sectioning of specimens is typically l...

    Authors: David J. Thomas, Jordan Rainbow and Laura E. Bartley
    Citation: Plant Methods 2023 19:12
  35. Crop breeding based on root system architecture (RSA) optimization is an essential factor for improving crop production in developing countries. Identification, evaluation, and selection of root traits of soil...

    Authors: Qian Wu, Jie Wu, Pengcheng Hu, Weixin Zhang, Yuntao Ma, Kun Yu, Yan Guo, Jing Cao, Huayong Li, Baiming Li, Yuyang Yao, Hongxin Cao and Wenyu Zhang
    Citation: Plant Methods 2023 19:11
  36. The number of soybean pods is one of the most important indicators of soybean yield, pod counting is crucial for yield estimation, cultivation management, and variety breeding. Counting pods manually is slow a...

    Authors: Shuai Xiang, Siyu Wang, Mei Xu, Wenyan Wang and Weiguo Liu
    Citation: Plant Methods 2023 19:8

    The Correction to this article has been published in Plant Methods 2023 19:45

  37. Karst vegetation is of great significance for ecological restoration in karst areas. Vegetation Indices (VIs) are mainly related to plant yield which is helpful to understand the status of ecological restorati...

    Authors: Wen Pan, Xiaoyu Wang, Yan Sun, Jia Wang, Yanjie Li and Sheng Li
    Citation: Plant Methods 2023 19:7
  38. As a result of the technological progress, the use of sensors for crop survey has substantially increased, generating valuable information for modelling agricultural data. Plant spectroscopy jointly with stati...

    Authors: Rosa Angela Pacheco-Gil, Ciro Velasco-Cruz, Paulino Pérez-Rodríguez, Juan Burgueño, Sergio Pérez-Elizalde, Francelino Rodrigues, Ivan Ortiz-Monasterio, David Hebert del Valle-Paniagua and Fernando Toledo
    Citation: Plant Methods 2023 19:6
  39. Cotton has tremendous economic value worldwide; however, its allopolyploid nature and time-consuming transformation methods have hampered the development of cotton functional genomics. The protoplast system ha...

    Authors: Ke Zhang, Shanhe Liu, Yunze Fu, Zixuan Wang, Xiubo Yang, Wenjing Li, Caihua Zhang, Dongmei Zhang and Jun Li
    Citation: Plant Methods 2023 19:5
  40. Cassava is the most cultivated and consumed root crop in the world. One of the major constraints to the cassava value chain is the short shelf life of cassava storage roots which is primarily due to the so-cal...

    Authors: Ima M. Zainuddin, Brieuc Lecart, Enny Sudarmonowati and Hervé Vanderschuren
    Citation: Plant Methods 2023 19:4
  41. This study presents a methodology for a high-throughput digitization and quantification process of plant cell walls characterization, including the automated development of two-dimensional finite element model...

    Authors: Andrew Sayad, Yusuf Oduntan, Norbert Bokros, Seth DeBolt, Alice Benzecry, Daniel J. Robertson and Christopher J. Stubbs
    Citation: Plant Methods 2023 19:3

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