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  1. The parasitic plant Orobanche cumana is one of the most important threats to sunflower crops in Europe. Resistant sunflower varieties have been developed, but new O. cumana races have evolved and have overcome in...

    Authors: A. Le Ru, G. Ibarcq, M.- C. Boniface, A. Baussart, S. Muños and M. Chabaud
    Citation: Plant Methods 2021 17:80
  2. Sugarcane (Saccharum officinarum L.) is an economically important crop with stalks as the harvest organs. Improvement in stalk quality is deemed a promising strategy for enhancing sugarcane production. However, t...

    Authors: Maoyao Wang, Xinru Li, Yinjuan Shen, Muhammad Adnan, Le Mao, Pan Lu, Qian Hu, Fuhong Jiang, Muhammad Tahir Khan, Zuhu Deng, Baoshan Chen, Jiangfeng Huang and Muqing Zhang
    Citation: Plant Methods 2021 17:76
  3. Jujube is a typical fruit tree species from China. ‘Muzao’, a cracking-susceptible cultivar, and ‘Linhuang No. 1’, a cracking-resistant cultivar, were selected in a previous study as contrasting research mater...

    Authors: Na Li, Yuqin Song, Jie Li, Ruijie Hao, Xinxin Feng and Liulin Li
    Citation: Plant Methods 2021 17:75
  4. Cassava is an important crop for food security and industry in the least-developed and developing countries. The completion of the cassava genome sequence and identification of large numbers of candidate genes...

    Authors: Decai Tuo, Peng Zhou, Pu Yan, Hongguang Cui, Yang Liu, He Wang, Xiukun Yang, Wenbin Liao, Di Sun, Xiaoying Li and Wentao Shen
    Citation: Plant Methods 2021 17:74
  5. Agrobacterium-mediated genetic transformation is a widely used and efficient technique for gene functional research in crop breeding and plant biology. While in some plant species, including soybean, genetic tran...

    Authors: Yuanyuan Cheng, Xiaoli Wang, Li Cao, Jing Ji, Tengfei Liu and Kaixuan Duan
    Citation: Plant Methods 2021 17:73
  6. Surface roughness has a significant effect on leaf wettability. Consequently, it influences the efficiency and effectiveness of pesticide application. Therefore, roughness measurement of leaf surface offers su...

    Authors: Zeqing Zhang, Fei Liu, Zhenjiang Zhou, Yong He and Hui Fang
    Citation: Plant Methods 2021 17:72
  7. CRISPR/Cas9 is a rapidly developing genome editing technology in various biological systems due to its efficiency, portability, simplicity and versatility. This editing technology has been successfully applied...

    Authors: Wang Wang, Jiangmiao Liu, Hai Wang, Tong Li and Huien Zhao
    Citation: Plant Methods 2021 17:71
  8. Stipagrostis pennata (Trin.) De Winter is an important species for fixing sand in shifting and semi-fixed sandy lands, for grazing, and potentially as a source of lignocellulose fibres for pulp and paper industry...

    Authors: Masoumeh Asadi-Aghbolaghi, Beata Dedicova, Sonali Sachi Ranade, Kim-Cuong Le, Farzad Sharifzadeh, Mansoor Omidi and Ulrika Egertsdotter
    Citation: Plant Methods 2021 17:70
  9. Obtaining instantaneous gas exchanges data is fundamental to gain information on photosynthesis. Leaf level data are reliable, but their scaling up to canopy scale is difficult as they are acquired in standard...

    Authors: Nicole Salvatori, Alberti Giorgio, Onno Muller, Uwe Rascher and Alessandro Peressotti
    Citation: Plant Methods 2021 17:69
  10. Accurate and efficient measurement of the diameter at breast height (DBH) of individual trees is essential for forest inventories, ecological management, and carbon budget estimation. However, traditional diam...

    Authors: Chuangye Song, Bin Yang, Lin Zhang and Dongxiu Wu
    Citation: Plant Methods 2021 17:67
  11. Protocols for Agrobacterium rhizogenes-mediated hairy root transformation of the model legume Lotus japonicus have been established previously. However, little efforts were made in the past to quantify and improv...

    Authors: Yan Wang, Feng Yang, Peng-Fei Zhu, Asaf Khan, Zhi-Ping Xie and Christian Staehelin
    Citation: Plant Methods 2021 17:66
  12. The study of plant phenotype by deep learning has received increased interest in recent years, which impressive progress has been made in the fields of plant breeding. Deep learning extremely relies on a large...

    Authors: Ruisong Zhang, Ye Tian, Junmei Zhang, Silan Dai, Xiaogai Hou, Jue Wang and Qi Guo
    Citation: Plant Methods 2021 17:65
  13. To enforce timber import laws and perform timber species identification, the identity of the botanical species must be well-defined. Since the Sapotaceae family is known as a taxonomically challenging family, ...

    Authors: V. Deklerck, E. Price, S. Vanden Abeele, K. Lievens, E. Espinoza and H. Beeckman
    Citation: Plant Methods 2021 17:64
  14. Astragalus mongholicus Bunge is an important medicinal plant used in traditional Chinese medicine. It is rich in isoflavonoids and triterpenoid saponins. Although these active constituents of A. mongholicus have ...

    Authors: Minzhen Yin, Shanshan Chu, Tingyu Shan, Liangping Zha and Huasheng Peng
    Citation: Plant Methods 2021 17:61
  15. The fraction of intercepted photosynthetically active radiation (fPARi) is typically described with a non-linear function of leaf area index (LAI) and k, the light extinction coefficient. The parameter k is used ...

    Authors: Josefina Lacasa, Trevor J. Hefley, María E. Otegui and Ignacio A. Ciampitti
    Citation: Plant Methods 2021 17:60
  16. Taraxacum officinale, or the common dandelion, is a widespread perennial species recognized worldwide as a common lawn and garden weed. Common dandelion is also cultivated for use in teas, as edible greens, and f...

    Authors: Kasia Dinkeloo, Araceli Maria Cantero, Inyup Paik, Alexa Vulgamott, Andrew D Ellington and Alan Lloyd
    Citation: Plant Methods 2021 17:59
  17. Epicuticular wax (EW) is the first line of defense in plants for protection against biotic and abiotic factors in the environment. In wheat, EW is associated with resilience to heat and drought stress, however...

    Authors: Fátima Camarillo-Castillo, Trevis D. Huggins, Suchismita Mondal, Matthew P. Reynolds, Michael Tilley and Dirk B. Hays
    Citation: Plant Methods 2021 17:58
  18. Water content variation during plant growth is one of the most important monitoring parameters in plant studies. Conventional parameters (such as dry weight) are unreliable; thus, the development of rapid, acc...

    Authors: Yunjeong Yang, Ji Eun Kim, Hak Jin Song, Eun Bin Lee, Yong-Keun Choi, Jeong Wook Jo, Hyeon Jin Jeon, Ho Hyun Kim, Kwang Jin Kim and Hyung Joo Kim
    Citation: Plant Methods 2021 17:56
  19. Water uptake is essential for seed germination. However, Gleditsia sinensis seeds have a water-impermeable seed coat, which is beneficial for its adaption to the environment, but prohibits its germination without...

    Authors: Mingwei Zhu, Song Dai, Qiuyue Ma and Shuxian Li
    Citation: Plant Methods 2021 17:55
  20. Annotating gene structures and functions to genome assemblies is necessary to make assembly resources useful for biological inference. Gene Ontology (GO) term assignment is the most used functional annotation ...

    Authors: Kokulapalan Wimalanathan and Carolyn J. Lawrence-Dill
    Citation: Plant Methods 2021 17:54
  21. A variety of basic and applied research programs in plant biology require the accurate and reliable determination of plant tissue cold hardiness. Over the past 50 years, the electrolyte leakage method has emer...

    Authors: Alisson P. Kovaleski and Jake J. Grossman
    Citation: Plant Methods 2021 17:53
  22. Fractional vegetation cover (FVC) is an important parameter for evaluating crop-growth status. Optical remote-sensing techniques combined with the pixel dichotomy model (PDM) are widely used to estimate cropla...

    Authors: Jibo Yue, Wei Guo, Guijun Yang, Chengquan Zhou, Haikuan Feng and Hongbo Qiao
    Citation: Plant Methods 2021 17:51
  23. To accurately estimate winter wheat leaf area index (LAI) using unmanned aerial vehicle (UAV) hyperspectral imagery is crucial for crop growth monitoring, fertilization management, and development of precision...

    Authors: Juanjuan Zhang, Tao Cheng, Wei Guo, Xin Xu, Hongbo Qiao, Yimin Xie and Xinming Ma
    Citation: Plant Methods 2021 17:49
  24. Maize (Zea mays L.) is one of the most important food sources in the world and has been one of the main targets of plant genetics and phenotypic research for centuries. Observation and analysis of various morp...

    Authors: Shuo Zhou, Xiujuan Chai, Zixuan Yang, Hongwu Wang, Chenxue Yang and Tan Sun
    Citation: Plant Methods 2021 17:48
  25. Circadian rhythms regulate several physiological and developmental processes of plants. Hence, the identification of genes with the underlying circadian rhythmic features is pivotal. Though computational metho...

    Authors: Prabina Kumar Meher, Ansuman Mohapatra, Subhrajit Satpathy, Anuj Sharma, Isha Saini, Sukanta Kumar Pradhan and Anil Rai
    Citation: Plant Methods 2021 17:46
  26. The paper provides studies on the structure of planted small-leaved lime (Tilia cordata Mill.) in the conditions of the Bashkir Cis-Urals. This work aimed to analyze their assortment by diameters and compile appr...

    Authors: Aydar Gabdelkhakov, Zagir Rakhmatullin, Maria Martynova, Ilyas Fazlutdinov and Ilnur Mullagaleev
    Citation: Plant Methods 2021 17:45
  27. How to control the physical damage during maize kernel harvesting is a major problem for both mechanical designers and plant breeders. A limitation of addressing this problem is lacking a reliable method for a...

    Authors: Yuan Su, Yang Xu, Tao Cui, Xiaojun Gao, Guoyi Xia, Yibo Li, Mengmeng Qiao and Yingbo Yu
    Citation: Plant Methods 2021 17:44
  28. Syntrichia caninervis is a typical desiccation tolerant moss that is a dominant species forming biological soil crusts in the Gurbantunggut Desert. This study investigated the effect of different explants on rege...

    Authors: Xiujin Liu, Ping Zhou, Xiaoshuang Li and Daoyuan Zhang
    Citation: Plant Methods 2021 17:42
  29. Investigating plant mechanisms to tolerate freezing temperatures is critical to developing crops with superior cold hardiness. However, the lack of imaging methods that allow the visualization of freezing even...

    Authors: Camilo Villouta, Benjamin L. Cox, Beth Rauch, Beth Ann A. Workmaster, Kevin W. Eliceiri and Amaya Atucha
    Citation: Plant Methods 2021 17:41
  30. Crop yield is dependent on climate conditions, which are becoming both more variable and extreme in some areas of the world as a consequence of global climate change. Increased precipitation and flooding event...

    Authors: Alexandra Miricescu, Tomás Byrne, Catherine M. Doorly, Carl K. Y. Ng, Susanne Barth and Emmanuelle Graciet
    Citation: Plant Methods 2021 17:40
  31. X-ray computed tomography is acknowledged as a powerful tool for the study of root system architecture of plants growing in soil. In this paper, we improved the original root segmentation algorithm “Rootine” a...

    Authors: Maxime Phalempin, Eva Lippold, Doris Vetterlein and Steffen Schlüter
    Citation: Plant Methods 2021 17:39
  32. Wood basic density (WBD) is one of the most crucial wood property in tree and mainly determined the end use of wood for industry. However, the measurement WBD is time- and cost-consuming, which an alternativel...

    Authors: Yanjie Li, Wenjian Liu, Ruishu Cao, Zifeng Tan, Jun Liu and Jingmin Jiang
    Citation: Plant Methods 2021 17:35
  33. Plant traits related to nutrition have an influential role in tree growth, tree production and nutrient cycling. Therefore, the breeding program should consider the genetics of the traits. However, the measure...

    Authors: Jun Liu, Yang Sun, Wenjian Liu, Zifeng Tan, Jingmin Jiang and Yanjie Li
    Citation: Plant Methods 2021 17:33
  34. We report a method to estimate carbon assimilation based on isotope ratio-mass spectrometry (IRMS) of 13CO2 labeled plant tissue. Photosynthetic carbon assimilation is the principal experimental observable which ...

    Authors: Matthew E. Bergman, Diego González-Cabanelas, Louwrance P. Wright, Berkley J. Walker and Michael A. Phillips
    Citation: Plant Methods 2021 17:32
  35. In vitro culture of olive, as an economically valuable tree, has fundamentally a genotype-dependant low micropropagation rate which needs to be improved in already established and newly released cultivars. Var...

    Authors: Leila Mirzaei, Abbas Yadollahi, Maryam Jafarkhani Kermani, Masoud Naderpour, Ali Asghar Zeinanloo, Maryam Farsi and Dariush Davoodi
    Citation: Plant Methods 2021 17:31

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