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  1. Seedling growth is an early phase of plant development highly susceptible to environmental factors such as soil nitrogen (N) availability or presence of seed-borne pathogens. Whereas N plays a central role in ...

    Authors: Thibault Barrit, Claire Campion, Sophie Aligon, Julie Bourbeillon, David Rousseau, Elisabeth Planchet and Béatrice Teulat
    Citation: Plant Methods 2022 18:131
  2. The technology of cotton defoliation is essential for mechanical cotton harvesting. Agricultural unmanned aerial vehicle (UAV) spraying has the advantages of low cost, high efficiency and no mechanical damage ...

    Authors: Jinyong Wu, Sheng Wen, Yubin Lan, Xuanchun Yin, Jiantao Zhang and Yufeng Ge
    Citation: Plant Methods 2022 18:129
  3. As complete and accurate genome sequences are becoming easier to obtain, more researchers wish to get one or more of them to support their research endeavors. Reliable and well-documented sequence assembly wor...

    Authors: Marina Püpke Marone, Harmeet Chawla Singh, Curtis J. Pozniak and Martin Mascher
    Citation: Plant Methods 2022 18:128
  4. High-throughput phenotyping is crucial for the genetic and molecular understanding of adaptive root system development. In recent years, imaging automata have been developed to acquire the root system architec...

    Authors: Romain Fernandez, Amandine Crabos, Morgan Maillard, Philippe Nacry and Christophe Pradal
    Citation: Plant Methods 2022 18:127
  5. Our understanding of the physiological responses of rice inflorescence (panicle) to environmental stresses is limited by the challenge of accurately determining panicle photosynthetic parameters and their impa...

    Authors: Jaspinder Singh Dharni, Balpreet Kaur Dhatt, Puneet Paul, Tian Gao, Tala Awada, Harel Bacher, Zvi Peleg, Paul Staswick, Jason Hupp, Hongfeng Yu and Harkamal Walia
    Citation: Plant Methods 2022 18:126
  6. Grasses internodes are made of distinct tissues such as vascular bundles, epidermis, rind and pith. The histology of grasses stem was largely revisited recently taking advantage of the development of microscop...

    Authors: P.-L. Lopez-Marnet, S. Guillaume, V. Méchin and M. Reymond
    Citation: Plant Methods 2022 18:125
  7. Chlorophyll and carotenoids, the most widely distributed lipophilic pigments in plants, contribute to fruit coloration during development and ripening. These pigments are assembled with pigment-protein complex...

    Authors: Jinli Gong, Hang Zhang, Yunliu Zeng, Yunjiang Cheng, Xuepeng Sun and Pengwei Wang
    Citation: Plant Methods 2022 18:124
  8. Acidic phytohormones are small molecules controlling many physiological functions in plants. A comprehensive picture of their profiles including the active forms, precursors and metabolites provides an importa...

    Authors: Jitka Široká, Federica Brunoni, Aleš Pěnčík, Václav Mik, Asta Žukauskaitė, Miroslav Strnad, Ondřej Novák and Kristýna Floková
    Citation: Plant Methods 2022 18:122
  9. Commonly, several traits are assessed in agronomic experiments to better understand the factors under study. However, it is also common to see that even when several traits are available, researchers opt to fo...

    Authors: Tiago Olivoto, Maria I. Diel, Denise Schmidt and Alessandro D. Lúcio
    Citation: Plant Methods 2022 18:121
  10. Cytokinins are a class of phytohormone that play a crucial role in the development of plants. They are involved in the regulation of nearly every aspect of plant growth, from germination to senescence. The rol...

    Authors: Lachlan Dow, Russell A. Barrow, Rosemary G. White and Ulrike Mathesius
    Citation: Plant Methods 2022 18:120
  11. Phytochemicals or secondary metabolites are low molecular weight organic compounds with little function in plant growth and development. Nevertheless, the metabolite diversity govern not only the phenetics of ...

    Authors: Muhammad-Redha Abdullah-Zawawi, Nisha Govender, Mohammad Bozlul Karim, Md. Altaf-Ul-Amin, Shigehiko Kanaya and Zeti-Azura Mohamed-Hussein
    Citation: Plant Methods 2022 18:118
  12. Researchers interested in the seed size of rapeseed need to quantify the cell size and number of cells in the seed coat, embryo and silique wall. Scanning electron microscope-based methods have been demonstrat...

    Authors: Yushun Jiao, Baoling Liang, Guangsheng Yang, Qiang Xin and Dengfeng Hong
    Citation: Plant Methods 2022 18:117
  13. Soybean gene functions cannot be easily interrogated through transgenic disruption (knock-out) of genes-of-interest, or transgenic overexpression of proteins-of-interest, because soybean transformation is time...

    Authors: Fides Angeli Zaulda, Seung Hyun Yang, Junping Han, Sizolwenkosi Mlotshwa, Anne Dorrance and Feng Qu
    Citation: Plant Methods 2022 18:116
  14. Living cells maintain and adjust structural and functional integrity by continual synthesis and degradation of metabolites and macromolecules. The maintenance and adjustment of thylakoid membrane involve turno...

    Authors: Anh Thi-Mai Banh, Björn Thiele, Antonia Chlubek, Thomas Hombach, Einhard Kleist and Shizue Matsubara
    Citation: Plant Methods 2022 18:114
  15. As one of the most widely planted fruit trees in southern China, citrus occupies an important position in the agriculture field and forestry economy in China. There are many kinds of citrus diseases. If citrus...

    Authors: Ruoli Yang, Tingjing Liao, Peirui Zhao, Wenhua Zhou, Mingfang He and Liujun Li
    Citation: Plant Methods 2022 18:113
  16. Defining interactions of bacteria in the rhizosphere (encompassing the area near and on the plant root) is important to understand how they affect plant health. Some rhizosphere bacteria, including plant growt...

    Authors: Amanda Rosier and Harsh P. Bais
    Citation: Plant Methods 2022 18:112
  17. According to the most popular definition, a biostimulant is any substance or microorganism applied to plants with the aim to enhance nutrition efficiency, abiotic stress tolerance and/or crop quality traits, r...

    Authors: Patricia Benito, Daniele Ligorio, Javier Bellón, Lynne Yenush and José M. Mulet
    Citation: Plant Methods 2022 18:111
  18. Phenomic prediction has been defined as an alternative to genomic prediction by using spectra instead of molecular markers. A reflectance spectrum provides information on the biochemical composition within a t...

    Authors: Charlotte Brault, Juliette Lazerges, Agnès Doligez, Miguel Thomas, Martin Ecarnot, Pierre Roumet, Yves Bertrand, Gilles Berger, Thierry Pons, Pierre François, Loïc Le Cunff, Patrice This and Vincent Segura
    Citation: Plant Methods 2022 18:108
  19. The allocation of non-structural carbohydrates (NSCs) plays a critical role in the physiology and metabolism of tree growth and survival defense. However, little is known about the allocation of NSC after cont...

    Authors: Yanjie Li, Honggang Sun, Thiago de Paula Protásio, Paulo Ricardo Gherardi Hein and Baoguo Du
    Citation: Plant Methods 2022 18:107
  20. Cowpea is a dryland crop with potential to improve food security in sub-Saharan Africa, where it is mostly produced and consumed. Contemporary plant improvement technologies, including genome editing, marker-a...

    Authors: Offiong Ukpong Edet and Takayoshi Ishii
    Citation: Plant Methods 2022 18:106
  21. Genome complexity is largely linked to diversification and crop innovation. Examples of regions with duplicated genes with relevant roles in agricultural traits are found in many crops. In both duplicated and ...

    Authors: Arnau Fiol, Federico Jurado-Ruiz, Elena López‑Girona and Maria José Aranzana
    Citation: Plant Methods 2022 18:105
  22. Eucommia ulmoides leaf (EUL), as a medicine and food homology plant, is a high-quality industrial raw material with great development potential for a valuable economic crop. There are many factors affecting the q...

    Authors: Lian Li, Zhi Min Li and Yuan Zhong Wang
    Citation: Plant Methods 2022 18:102
  23. As a rapid and non-destructive method, Near Infrared Spectroscopy is classically proposed to assess plant traits in many scientific fields, to observe enlarged genotype panels and to document the temporal kine...

    Authors: Héloïse Villesseche, Martin Ecarnot, Elsa Ballini, Ryad Bendoula, Nathalie Gorretta and Pierre Roumet
    Citation: Plant Methods 2022 18:100
  24. Quantification of gene expression such as RNA-Seq is a popular approach to study various biological phenomena. Despite the development of RNA-Seq library preparation methods and sequencing platforms in the las...

    Authors: Makoto Kashima, Mari Kamitani, Yasuyuki Nomura, Natsumi Mori-Moriyama, Shigeyuki Betsuyaku, Hiromi Hirata and Atsushi J. Nagano
    Citation: Plant Methods 2022 18:99
  25. Genome editing is essential for crop molecular breeding. However, gene editing in turnip (Brassica rapa var. rapa) have not been reported owing to the very low transformation efficiency.

    Authors: Yuanyuan Liu, Li Zhang, Cheng Li, Yunqiang Yang, Yuanwen Duan, Yongping Yang and Xudong Sun
    Citation: Plant Methods 2022 18:98
  26. Photosynthesis close interacts with respiration and nitrogen assimilation, which determine the photosynthetic efficiency of a leaf. Accurately quantifying the metabolic fluxes in photosynthesis, respiration an...

    Authors: Qiming Tang, Qingfeng Song, Xiaoxiang Ni, Zai Shi, Genyun Chen and Xinguang Zhu
    Citation: Plant Methods 2022 18:97
  27. Characterizing plant genetic resources and their response to the environment through accurate measurement of relevant traits is crucial to genetics and breeding. Spatial organization of the maize ear provides ...

    Authors: V. Oury, T. Leroux, O. Turc, R. Chapuis, C. Palaffre, F. Tardieu, S. Alvarez Prado, C. Welcker and S. Lacube
    Citation: Plant Methods 2022 18:96
  28. The demand for productive economic plant resources is increasing with the continued growth of the human population. Ancient Pu’er tea trees [Camellia sinensis var. assamica (J. W. Mast.) Kitam.] are an important ...

    Authors: Shuqiao Zhang, Wendou Liu, Xinmeng Cheng, Zizhi Wang, Fengjun Yuan, Wengui Wu and Shengxi Liao
    Citation: Plant Methods 2022 18:95
  29. Bulk segregant analysis (BSA) can help identify quantitative trait loci (QTLs), but this may result in substantial bycatch of functionally irrelevant genes.

    Authors: Rutger A. Vos, Catharina A. M. van der Veen-van Wijk, M. Eric Schranz, Klaas Vrieling, Peter G. L. Klinkhamer and Frederic Lens
    Citation: Plant Methods 2022 18:92
  30. Clubroot of canola (Brassica napus), caused by the soilborne pathogen Plasmodiophora brassicae, has become a serious threat to canola production in Canada. The deployment of clubroot-resistant (CR) cultivars is t...

    Authors: Heather H. Tso, Leonardo Galindo-González, Troy Locke and Stephen E. Strelkov
    Citation: Plant Methods 2022 18:91
  31. The application of autopilot technology is conductive to achieving path planning navigation and liberating labor productivity. In addition, the self-driving vehicles can drive according to the growth state of ...

    Authors: Xihuizi Liang, Bingqi Chen, Chaojie Wei and Xiongchu Zhang
    Citation: Plant Methods 2022 18:90
  32. Salicylic acid (SA) is one of the plant hormones, which plays crucial roles in signaling transduction in plant growth, disease resistance, and leaf senescence. Arabidopsis (Arabidopsis thaliana) SA 3-hydroxylase ...

    Authors: Zilin Cai, Hao Guo, Shijing Shen, Qilu Yu, Jinbin Wang, Engao Zhu, Pinghua Zhang, Lili Song, Yanjun Zhang and Kewei Zhang
    Citation: Plant Methods 2022 18:89
  33. Jubaea chilensis (Molina) Baillon, is a uniquely large palm species endemic to Chile. It is under threatened status despite its use as an ornamental species throughout the world. This research seeks to identify t...

    Authors: Eduardo Guzmán, M. Paulina Fernández, José-Antonio Alcalde, Samuel Contreras, Pasi Raumonen, Lorenzo Picco, Cristián Montalba and Cristián Tejos
    Citation: Plant Methods 2022 18:88
  34. Quercetin is one of the most important bioflavonoids having positive effects on the biological processes and human health. Typically, it is extracted from plant matrices using conventional methods such as mace...

    Authors: Veronika Pilařová, Lukáš Kuda, Hana Kočová Vlčková, Lucie Nováková, Shubhpriya Gupta, Manoj Kulkarni, František Švec, Johannes Van Staden and Karel Doležal
    Citation: Plant Methods 2022 18:87
  35. Methods to accurately quantify disease severity are fundamental to plant pathogen interaction studies. Commonly used methods include visual scoring of disease symptoms, tracking pathogen growth in planta over tim...

    Authors: Kiona Elliott, Jeffrey C. Berry, Hobin Kim and Rebecca S. Bart
    Citation: Plant Methods 2022 18:86
  36. Elaeagnus angustifolia L. is a deciduous tree in the family Elaeagnaceae. It is widely used to study abiotic stress tolerance in plants and to improve desertification-affected land because of its ability to withs...

    Authors: Yunfei Mao, Xueli Cui, Haiyan Wang, Xin Qin, Yangbo Liu, Yijun Yin, Xiafei Su, Juan Tang, Fengling Wang, Fengwang Ma, Naibin Duan, Donglin Zhang, Yanli Hu, Wenli Wang, Shaochong Wei, Xiaoliu Chen…
    Citation: Plant Methods 2022 18:84
  37. The interaction between canopy and droplets is very important in the process of crop spraying. During the actual air-assisted application process, air-mist flow inevitably disturbs the leaves before droplets r...

    Authors: Yubin Cao, Te Xi, Lujiang Xu, Wei Qiu, Hongbin Guo, Xiaolan Lv and Chao Li
    Citation: Plant Methods 2022 18:80
  38. Many significant ecosystems, including important non-forest woody ecosystems such as the Cerrado (Brazilian savannah), are under threat from climate change, yet our understanding of how increasing temperatures...

    Authors: Georgina A. Werkmeister, David Galbraith, Emma Docherty, Camilla Silva Borges, Jairo Matos da Rocha, Paulo Alves da Silva, Beatriz Schwantes Marimon, Ben Hur Marimon-Junior, Oliver L. Phillips and Emanuel Gloor
    Citation: Plant Methods 2022 18:78

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