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  1. The phenotypic traits of leaves are the direct reflection of the agronomic traits in the growth process of leafy vegetables, which plays a vital role in the selection of high-quality leafy vegetable varieties....

    Authors: Ziran Ye, Xiangfeng Tan, Mengdi Dai, Xuting Chen, Yuanxiang Zhong, Yi Zhang, Yunjie Ruan and Dedong Kong
    Citation: Plant Methods 2024 20:22
  2. The leaf angle distribution (LAD) is an important structural parameter of agricultural crops that influences light interception, radiation fluxes and consequently plant performance. Therefore, LAD and its para...

    Authors: Marie Theiß, Angelina Steier, Uwe Rascher and Mark Müller-Linow
    Citation: Plant Methods 2024 20:21
  3. The study aimed to edit ethylene (ET) biosynthesis genes [1-aminocyclopropane-1-carboxylic acid (ACC) synthetase 1 (ACS1) and ACC oxidase 1 (ACO1)] in carnation using the CRISPR/Cas9 ribonucleoprotein (RNP) compl...

    Authors: Oluwaseun Suleimon Adedeji, Aung Htay Naing, Hyunhee Kang, Junping Xu, Mi Young Chung and Chang Kil Kim
    Citation: Plant Methods 2024 20:20
  4. Herbaria are becoming increasingly important as archives of biodiversity, and play a central role in taxonomic and biogeographic studies. There is also an ongoing interest in functional traits and the way they...

    Authors: Paul Kühn, Tobias Proß, Christine Römermann, Karsten Wesche and Helge Bruelheide
    Citation: Plant Methods 2024 20:19
  5. Investigations on plant-pathogen interactions require quantitative, accurate, and rapid phenotyping of crop diseases. However, visual assessment of disease symptoms is preferred over available numerical tools ...

    Authors: Laura Mathieu, Maxime Reder, Ali Siah, Aurélie Ducasse, Camilla Langlands-Perry, Thierry C. Marcel, Jean-Benoît Morel, Cyrille Saintenac and Elsa Ballini
    Citation: Plant Methods 2024 20:18
  6. The low efficiency of genetic transformation in Chinese cabbage (Brassica rapa L. ssp. pekinensis) is the key problem affecting functional verification. Particle bombardment is a widely used method along with the...

    Authors: Yujia Liu, Shujiang Zhang, Shifan Zhang, Hui Zhang, Guoliang Li, Rifei Sun and Fei Li
    Citation: Plant Methods 2024 20:17
  7. One of the levers towards alternative solutions to pesticides is to improve seed defenses against pathogens, but a better understanding is needed on the type and regulation of existing pathways during germinat...

    Authors: Benjamin Hubert, Muriel Marchi, Joseph Ly Vu, Camille Tranchant, Łukasz P. Tarkowski, Olivier Leprince and Julia Buitink
    Citation: Plant Methods 2024 20:16
  8. The number of seedlings is an important indicator that reflects the size of the wheat population during the seedling stage. Researchers increasingly use deep learning to detect and count wheat seedlings from u...

    Authors: Suwan Wang, Jianqing Zhao, Yucheng Cai, Yan Li, Xuerui Qi, Xiaolei Qiu, Xia Yao, Yongchao Tian, Yan Zhu, Weixing Cao and Xiaohu Zhang
    Citation: Plant Methods 2024 20:15
  9. The potential of plant-based sources of vernolic acid to provide agricultural producers with a market diversification opportunity and industrial manufacturers with a renewable, environmentally friendly chemica...

    Authors: Maram Istaitieh, Jim F. Todd, Rene C. Van Acker, Mohsen Yoosefzadeh-Najafabadi and Istvan Rajcan
    Citation: Plant Methods 2024 20:14
  10. The study of plant photosynthesis is essential for productivity and yield. Thanks to the development of high-throughput phenotyping (HTP) facilities, based on chlorophyll fluorescence imaging, photosynthetic t...

    Authors: Federico Jurado-Ruiz, Thu-Phuong Nguyen, Joseph Peller, María José Aranzana, Gerrit Polder and Mark G. M. Aarts
    Citation: Plant Methods 2024 20:11
  11. Cryopreservation makes it possible to preserve plant biodiversity for thousands of years in ex situ storage. The stepwise dehydration method is a simple and versatile cryopreservation technique based on the vi...

    Authors: Teresa Hazubska-Przybył, Mikołaj Krzysztof Wawrzyniak, Agata Obarska and Terezia Salaj
    Citation: Plant Methods 2024 20:10
  12. Lentil is an important pulse that contributes to global food security and the sustainability of farming systems. Hence, it is important to increase the production of this crop, especially in the context of cli...

    Authors: Mohammed Mitache, Aziz Baidani, Bouchaib Bencharki and Omar Idrissi
    Citation: Plant Methods 2024 20:9
  13. In drought periods, water use efficiency depends on the capacity of roots to extract water from deep soil. A semi-field phenotyping facility (RadiMax) was used to investigate above-ground and root traits in sp...

    Authors: Biructawit B. Tessema, Miguel A. Raffo, Xiangyu Guo, Simon F. Svane, Lene Krusell, Jens Due Jensen, Anja Karine Ruud, Marta Malinowska, Kristian Thorup-Kristensen and Just Jensen
    Citation: Plant Methods 2024 20:8
  14. Strategies to understand meiotic processes have relied on cytogenetic and mutant analysis. However, thus far in vitro meiosis induction is a bottleneck to laboratory-based plant breeding as factor(s) that swit...

    Authors: Tanner M. Cook, Eva Biswas, Somak Dutta, Siddique I. Aboobucker, Sara Hazinia and Thomas Lübberstedt
    Citation: Plant Methods 2024 20:7
  15. Water dropwort (Oenanthe javanica (Blume) DC), an aquatic perennial plant from the Apiaceae family, rich in dietary fibert, vitamins, and minerals. It usually grows in wet soils and water. Despite accumulating th...

    Authors: Zhen He, Shuangyu Sheng, Lingqi Wang, Tingting Dong, Kun Zhang and Liangjun Li
    Citation: Plant Methods 2024 20:6
  16. The measurement of root dielectric response is a useful non-destructive method to evaluate root growth and function. Previous studies tracked root development throughout the plant growing cycle by single-time ...

    Authors: Imre Cseresnyés, Anna Füzy, Sándor Kabos, Bettina Kelemen, Kálmán Rajkai and Tünde Takács
    Citation: Plant Methods 2024 20:5
  17. The aim of this study was to evaluate and characterize the mutations induced by two TALE-based approaches, double-strand break (DSB) induction by the FokI nuclease (mitoTALEN) and targeted base editing by the Ddd...

    Authors: Alessandro Nicolia, Nunzia Scotti, Nunzio D’Agostino, Giovanna Festa, Lorenza Sannino, Gaetano Aufiero, Shin-ichi Arimura and Teodoro Cardi
    Citation: Plant Methods 2024 20:4
  18. Populus simonii × P. nigra is an ideal material for studying the molecular mechanisms of woody plants. In recent years, research on Populus simonii × P. nigra has increasingly focused on the application of transg...

    Authors: Ping Yang, Yao Sun, Xin Sun, Yao Li and Lei Wang
    Citation: Plant Methods 2024 20:3
  19. The chloroplast genome (cp genome) is directly related to the study and analysis of molecular phylogeny and evolution of plants in the phylogenomics era. The cp genome, whereas, is highly plastic and exists as...

    Authors: Qiang Zhou, Xianlong Ding, Hongjie Wang, Zunaira Farooq, Liang Wang and Shouping Yang
    Citation: Plant Methods 2024 20:2
  20. Tea is the most popular beverage worldwide second only to water. Its demand is tremendously rising due to increased awareness of its medicinal importance. The quality and uses of tea depend on the tea-types wh...

    Authors: Abhishek Kumar, Nikhil Rawat, Shweta Thakur, Rohit Joshi and Shiv Shanker Pandey
    Citation: Plant Methods 2023 19:147

    The Correction to this article has been published in Plant Methods 2024 20:57

  21. Grapevine berries undergo asynchronous growth and ripening dynamics within the same bunch. Due to the lack of efficient methods to perform sequential non-destructive measurements on a representative number of ...

    Authors: Benoit Daviet, Christian Fournier, Llorenç Cabrera-Bosquet, Thierry Simonneau, Maxence Cafier and Charles Romieu
    Citation: Plant Methods 2023 19:146
  22. Crop pests reduce productivity, so managing them through early detection and prevention is essential. Data from various modalities are being used to predict crop diseases by applying machine learning methodolo...

    Authors: Sangyeon Lee and Choa Mun Yun
    Citation: Plant Methods 2023 19:145

    The Correction to this article has been published in Plant Methods 2024 20:24

  23. Understanding thiamin (thiamine; vitamin B1) metabolism in plants is crucial, as it impacts plant nutritional value as well as stress tolerance. Studies aimed at elucidating novel aspects of thiamin in plants rel...

    Authors: Simon Strobbe, Jana Verstraete, Teresa B. Fitzpatrick, Christophe Stove and Dominique Van Der Straeten
    Citation: Plant Methods 2023 19:144
  24. The Amaranthus genus contains at least 20 weedy and invasive species, including Amaranthus palmeri (palmer’s amaranth) and Amaranthus tuberculatus (tall waterhemp), two species of regulatory concern in North Amer...

    Authors: Maxime Murphy, Julia Hubert, Ruojing Wang and Leonardo Galindo-González
    Citation: Plant Methods 2023 19:143
  25. Plant defense activators offer advantages over pesticides by avoiding the emergence of drug-resistant pathogens. However, only a limited number of compounds have been reported. Reactive oxygen species (ROS) ac...

    Authors: Masayuki Kogoshi, Daiki Nishio, Nobutaka Kitahata, Hayato Ohwada, Kazuyuki Kuchitsu, Hideyuki Mizuno and Takamitsu Kurusu
    Citation: Plant Methods 2023 19:142
  26. Protein–protein interactions (PPIs) are heavily involved in many biological processes. Consequently, the identification of PPIs in the model plant Arabidopsis is of great significance to deeply understand plan...

    Authors: Kewei Zhou, Chenping Lei, Jingyan Zheng, Yan Huang and Ziding Zhang
    Citation: Plant Methods 2023 19:141
  27. For molecular breeding of future apples, wild apple (Malus sieversii), the primary progenitor of domesticated apples, provides abundant genetic diversity and disease-resistance traits. Valsa canker (caused by the...

    Authors: Xuejing Wen, Jiangxue Yuan, Tohir A. Bozorov, Abdul Waheed, Gulnaz Kahar, Yakupjan Haxim, Xiaojie Liu, Lili Huang and Daoyuan Zhang
    Citation: Plant Methods 2023 19:138
  28. Doubled haploid technology, which enables the generation of homozygous lines in a single step, is one of the modern tools being employed for accelerating breeding processes in different crops. In rice, a globa...

    Authors: Chaitanya Ghalagi, Malavalli Rajashekar Namratha, Kavita Kotyal, Shiva Prakash and Basavaiah Mohan Raju
    Citation: Plant Methods 2023 19:137
  29. Lily is a bulbous plant with an endogenous dormancy trait. Fine-tuning bulb dormancy release is still a challenge in the development of bulb storage technology. In this study, we identified three regulators of...

    Authors: Yajie Zhao, Wenqiang Pan, Yin Xin, Jingxiang Wu, Rong Li, Jinxin Shi, Shuo Long, Lianwei Qu, Yingdong Yang, Mingfang Yi and Jian Wu
    Citation: Plant Methods 2023 19:136
  30. Calcium oxalate (CaOx) is the most prevalent and widespread biomineral in plants and is involved in protective and/or defensive functions against abiotic stress factors. It is, however, expected that this func...

    Authors: Changying Li, Chunli Chen, Lihong Qin, Dengyue Zheng, Qian Du, Qiandong Hou and Xiaopeng Wen
    Citation: Plant Methods 2023 19:135
  31. The metrics for assessing the yield of crops in the field include the number of ears per unit area, the grain number per ear, and the thousand-grain weight. Typically, the ear number per unit area contributes ...

    Authors: Yangyang Gu, Hongxu Ai, Tai Guo, Peng Liu, Yongqing Wang, Hengbiao Zheng, Tao Cheng, Yan Zhu, Weixing Cao and Xia Yao
    Citation: Plant Methods 2023 19:134
  32. Thermography is a popular tool to assess plant water-use behavior, as plant temperature is influenced by transpiration rate, and is commonly used in field experiments to detect plant water deficit. Its applica...

    Authors: Stien Mertens, Lennart Verbraeken, Heike Sprenger, Sam De Meyer, Kirin Demuynck, Bernard Cannoot, Julie Merchie, Jolien De Block, Jonathan T. Vogel, Wesley Bruce, Hilde Nelissen, Steven Maere, Dirk Inzé and Nathalie Wuyts
    Citation: Plant Methods 2023 19:132
  33. In the past few years, there has been an explosion in single-cell transcriptomics datasets, yet in vivo confirmation of these datasets is hampered in plants due to lack of robust validation methods. Likewise, ...

    Authors: Tian Huang, Bruno Guillotin, Ramin Rahni, Kenneth D. Birnbaum and Doris Wagner
    Citation: Plant Methods 2023 19:131
  34. The evolution of plants to efficiently transport water and assimilates over long distances is a major evolutionary success that facilitated their growth and colonization of land. Vascular tissues, namely xylem...

    Authors: Karolina Kułak, Natalia Wojciechowska, Anna Samelak-Czajka, Paulina Jackowiak and Agnieszka Bagniewska-Zadworna
    Citation: Plant Methods 2023 19:129
  35. Survival rate (SR) is frequently used to compare drought tolerance among plant genotypes. While a variety of techniques for evaluating the stress status of plants under drought stress conditions have been deve...

    Authors: Thelma Y. Rico-Cambron, Elohim Bello-Bello, Octavio Martínez and Luis Herrera-Estrella
    Citation: Plant Methods 2023 19:127
  36. Object detection, size determination, and colour detection of images are tools commonly used in plant science. Key examples of this include identification of ripening stages of fruit such as tomatoes and the d...

    Authors: Harry Charles Wright, Frederick Antonio Lawrence, Anthony John Ryan and Duncan Drummond Cameron
    Citation: Plant Methods 2023 19:126
  37. Inflorescence properties such length, spikelet number, and their spatial distribution across the rachis, are fundamental indicators of seed productivity in grasses and have been a target of selection throughou...

    Authors: Joan Barreto Ortiz, Candice N. Hirsch, Nancy Jo Ehlke and Eric Watkins
    Citation: Plant Methods 2023 19:125
  38. Manual analysis of (mini-)rhizotron (MR) images is tedious. Several methods have been proposed for semantic root segmentation based on homogeneous, single-source MR datasets. Recent advances in deep learning (...

    Authors: Pavel Baykalov, Bart Bussmann, Richard Nair, Abraham George Smith, Gernot Bodner, Ofer Hadar, Naftali Lazarovitch and Boris Rewald
    Citation: Plant Methods 2023 19:122

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