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  1. Glandular trichomes, often referred to as “phytochemical factories”, plays a crucial role in plant growth and metabolism. As the site for secretion and storage, the development of glandular trichomes is relate...

    Authors: Zheng Jiang, Peina Zhou, Yongfang Shao, Qianqian Zhang, Wei Yue, Cheng Qu and Qinan Wu
    Citation: Plant Methods 2023 19:88
  2. Efficient and site-specific weed management is a critical step in many agricultural tasks. Image captures from drones and modern machine learning based computer vision methods can be used to assess weed infest...

    Authors: Nikita Genze, Maximilian Wirth, Christian Schreiner, Raymond Ajekwe, Michael Grieb and Dominik G. Grimm
    Citation: Plant Methods 2023 19:87
  3. Rust is a damaging disease affecting vital crops, including pea, and identifying highly resistant genotypes remains a challenge. Accurate measurement of infection levels in large germplasm collections is cruci...

    Authors: Salvador Osuna-Caballero, Tiago Olivoto, Manuel A. Jiménez-Vaquero, Diego Rubiales and Nicolas Rispail
    Citation: Plant Methods 2023 19:86
  4. High-purity RNA serves as the basic requirement for downstream molecular analysis of plant species, especially the differential expression of genes to various biotic and abiotic stimuli. But, the extraction of...

    Authors: Shina Sasi, Saranya Krishnan, Preshobha Kodackattumannil, Aysha AL Shamisi, Maitha Aldarmaki, Geetha Lekshmi, Martin Kottackal and Khaled M. A. Amiri
    Citation: Plant Methods 2023 19:84
  5. Tar spot of corn is a significant and spreading disease in the continental U.S. and Canada caused by the obligate biotrophic fungus Phyllachora maydis. As of 2023, tar spot had been reported in 18 U.S. states and...

    Authors: José E. Solórzano, Shea E. Issendorf, Milton T. Drott, Jill C. Check, Emily M. Roggenkamp, C. D. Cruz, Nathan M. Kleczewski, Carlos C. Gongóra-Canul and Dean K. Malvick
    Citation: Plant Methods 2023 19:83
  6. Pumpkin seeds are major oil crops with high nutritional value and high oil content. The collection and identification of different pumpkin germplasm resources play a significant role in the realization of prec...

    Authors: Xiyao Li, Xuping Feng, Hui Fang, Ningyuan Yang, Guofeng Yang, Zeyu Yu, Jia Shen, Wei Geng and Yong He
    Citation: Plant Methods 2023 19:82
  7. Though multicolour labelling methods allow the routine detection of a wide range of fluorescent (immuno)probe types in molecular cytogenetics, combined applications for the simultaneous in situ detection of pr...

    Authors: Diána Makai, Edit Mihók, Dávid Polgári, András Cseh, Andrea Lenykó-Thegze, Adél Sepsi and László Sági
    Citation: Plant Methods 2023 19:80
  8. Large-scale data on the photosynthetic characteristics of whole crop canopy is crucial for improving yield. However, current data collection methods remain challenging, and the time constraints associated with...

    Authors: Yu Hyun Moon, Ui Jeong Woo, Ha Seon Sim, Tae Yeon Lee, Ha Rang Shin, Jung Su Jo and Sung Kyeom Kim
    Citation: Plant Methods 2023 19:79
  9. Plant virus vectors designed for virus-mediated protein overexpression (VOX), virus-induced gene silencing (VIGS), and genome editing (VIGE) provide rapid and cost-effective tools for functional genomics studi...

    Authors: Decai Tuo, Yuan Yao, Pu Yan, Xin Chen, Feihong Qu, Weiqian Xue, Jinping Liu, Hua Kong, Jianchun Guo, Hongguang Cui, Zhaoji Dai and Wentao Shen
    Citation: Plant Methods 2023 19:78
  10. The morphological structure phenotype of maize tassel plays an important role in plant growth, reproduction, and yield formation. It is an important step in the distinctness, uniformity, and stability (DUS) te...

    Authors: Wenqi Zhang, Sheng Wu, Weiliang Wen, Xianju Lu, Chuanyu Wang, Wenbo Gou, Yuankun Li, Xinyu Guo and Chunjiang Zhao
    Citation: Plant Methods 2023 19:76
  11. Verticillium wilt is the major disease of cotton, which would cause serious yield reduction and economic losses, and the identification of cotton verticillium wilt is of great significance to cotton research. ...

    Authors: Zhihao Lu, Shihao Huang, Xiaojun Zhang, Yuxuan shi, Wanneng Yang, Longfu Zhu and Chenglong Huang
    Citation: Plant Methods 2023 19:75
  12. A commonly used approach to study the interaction of two proteins of interest (POIs) in vivo is measuring Förster Resonance Energy Transfer (FRET). This requires the expression of the two POIs fused to two flu...

    Authors: Jan Eric Maika, Benedikt Krämer, Vivien I. Strotmann, Frank Wellmer, Stefanie Weidtkamp-Peters, Yvonne Stahl and Rüdiger Simon
    Citation: Plant Methods 2023 19:73
  13. Alternative splicing (AS) of mRNA plays an important roles in transcriptome diversity, involving regulation of plant growth and stress response. Understanding the variation of AS events underlying GWAS loci in...

    Authors: Siyuan Wang, Hongyu Wu, Yongyan Zhao, Luyao Wang, Xueying Guan and Ting Zhao
    Citation: Plant Methods 2023 19:72
  14. Investigations into the growth and self-organization of plant roots is subject to fundamental and applied research in various areas such as botany, agriculture, and soil science. The growth activity of the pla...

    Authors: Yalda Davoudpour, Steffen Kümmel, Niculina Musat, Hans Hermann Richnow and Matthias Schmidt
    Citation: Plant Methods 2023 19:71
  15. Cunninghamia lanceolata (Chinese fir), is one of the most important timber trees in China. With the global warming, to develop new resistant varieties to drought or heat stress has become an essential task for br...

    Authors: Dong Xing, Yulin Wang, Penghui Sun, Huahong Huang and Erpei Lin
    Citation: Plant Methods 2023 19:66
  16. Forest aboveground biomass (AGB) is not only the basis for estimating forest carbon storage, but also an important parameter for evaluating forest carbon cycle contribution and forest ecological function. Data...

    Authors: Yueting Wang, Xiang Jia, Guoqi Chai, Lingting Lei and Xiaoli Zhang
    Citation: Plant Methods 2023 19:65
  17. Modern computational modeling could provide the key to obtaining new insights into the mechanisms of maize stalk failure as well as suggesting new ways to improve stalk strength. However, a complete set of mec...

    Authors: Brandon Sutherland, Kirsten Steele, Joseph Carter and Douglas D. Cook
    Citation: Plant Methods 2023 19:64
  18. The role of beneficial microbes in mitigating plant abiotic stress has received considerable attention. However, the lack of a reproducible and relatively high-throughput screen for microbial contributions to ...

    Authors: Jun Hyung Lee, Leah H. Burdick, Bryan Piatkowski, Alyssa A. Carrell, Mitchel J. Doktycz, Dale A. Pelletier and David J. Weston
    Citation: Plant Methods 2023 19:63
  19. Common reed (Phragmites australis L.) is a highly productive wetland plant and a possible valuable resource of renewable biomass worldwide. For a sustainable management the exploitation of reed is beneficial b...

    Authors: Cristian Dragos Obreja, Daniela Laura Buruiana, Elena Mereuta, Alina Muresan, Alina Mihaela Ceoromila, Viorica Ghisman and Roxana Elena Axente
    Citation: Plant Methods 2023 19:61
  20. Computer vision technology is moving more and more towards a three-dimensional approach, and plant phenotyping is following this trend. However, despite its potential, the complexity of the analysis of 3D repr...

    Authors: Negin Harandi, Breght Vandenberghe, Joris Vankerschaver, Stephen Depuydt and Arnout Van Messem
    Citation: Plant Methods 2023 19:60
  21. RGB photographs are a powerful tool for dynamically estimating crop growth. Leaves are related to crop photosynthesis, transpiration, and nutrient uptake. Traditional blade parameter measurements were labor-in...

    Authors: Xiuni Li, Xiangyao Xu, Shuai Xiang, Menggen Chen, Shuyuan He, Wenyan Wang, Mei Xu, Chunyan Liu, Liang Yu, Weiguo Liu and Wenyu Yang
    Citation: Plant Methods 2023 19:59
  22. To gain a better understanding of bark layer structure and function, especially of the phloem fibres and their contribution to the posture control of trees, it is important to map the structural properties of ...

    Authors: Mira Viljanen, Sampo Muranen, Outi Kinnunen, Sebastian Kalbfleisch and Kirsi Svedström
    Citation: Plant Methods 2023 19:58
  23. Studying the relationships between rapeseed seed yield (SY) and its yield-related traits can assist rapeseed breeders in the efficient indirect selection of high-yielding varieties. However, since the conventi...

    Authors: Masoud Shahsavari, Valiollah Mohammadi, Bahram Alizadeh and Houshang Alizadeh
    Citation: Plant Methods 2023 19:57
  24. Global warming is a major challenge for plant survival and growth. Understanding the molecular mechanisms by which higher plants sense and adapt to upsurges in the ambient temperature is essential for developi...

    Authors: Anthony Guihur, Baptiste Bourgine, Mathieu E. Rebeaud and Pierre Goloubinoff
    Citation: Plant Methods 2023 19:56
  25. Isoprenoids are a very large class of metabolites playing a key role in plant physiological processes such as growth, stress resistance, fruit flavor, and color. In chloroplasts and chromoplasts, the diterpene...

    Authors: Wayne Zita, Venkatasalam Shanmugabalaji, Miguel Ezquerro, Manuel Rodriguez-Concepcion, Felix Kessler and Gaetan Glauser
    Citation: Plant Methods 2023 19:55
  26. The time between the appearance of successive leaves, or phyllochron, characterizes the vegetative development of annual plants. Hypothesis testing models, which allow the comparison of phyllochrons between ge...

    Authors: Sandra Plancade, Elodie Marchadier, Sylvie Huet, Adrienne Ressayre, Camille Noûs and Christine Dillmann
    Citation: Plant Methods 2023 19:54
  27. Application of hyperspectral imaging (HSI) and data analysis algorithms was investigated for early and non-destructive detection of Botrytis cinerea infection. Hyperspectral images were collected from laboratory-...

    Authors: Najmeh Haghbin, Adel Bakhshipour, Hemad Zareiforoush and Sedigheh Mousanejad
    Citation: Plant Methods 2023 19:53
  28. Environmental stress due to climate or pathogens is a major threat to modern agriculture. Plant genetic resistance to these stresses is one way to develop more resilient crops, but accurately quantifying plant...

    Authors: Changye Yang, Sriram Baireddy, Valérian Méline, Enyu Cai, Denise Caldwell, Anjali S. Iyer-Pascuzzi and Edward J. Delp
    Citation: Plant Methods 2023 19:52
  29. The advancements in unmanned aerial vehicle (UAV) technology have recently emerged as an effective, cost-efficient, and versatile solution for monitoring crop growth with high spatial and temporal precision. T...

    Authors: Yuxiang Wang, Zengling Yang, Gert Kootstra and Haris Ahmad Khan
    Citation: Plant Methods 2023 19:51

    The Correction to this article has been published in Plant Methods 2023 19:62

  30. Passion fruit is an essential commercial plant in the tropics and subtropics, which has lately seen a rise in demand for high-quality fruits and large-scale production. Generally, different species of passion ...

    Authors: Mohammad Aqa Mohammadi, Myat Hnin Wai, Hafiz Muhammad Rizwan, Abdul Qahar Qarluq, Mengjie Xu, Lulu Wang, Yan Cheng, Mohammad Aslam, Ping Zheng, Xiaomei Wang, Wenbin Zhang and Yuan Qin
    Citation: Plant Methods 2023 19:50
  31. A well-known method for evaluating plant resistance to insects is by measuring insect reproduction or oviposition. Whiteflies are vectors of economically important viral diseases and are, therefore, widely stu...

    Authors: Micha Gracianna Devi, Dan Jeric Arcega Rustia, Lize Braat, Kas Swinkels, Federico Fornaguera Espinosa, Bart M. van Marrewijk, Jochen Hemming and Lotte Caarls
    Citation: Plant Methods 2023 19:49
  32. Nitrogen (N), phosphorus (P), and potassium (K) contents are crucial quality indicators for forage in alpine natural grasslands and are closely related to plant growth and reproduction. One of the greatest cha...

    Authors: Xuanfan Zhang, Tiangang Liang, Jinlong Gao, Dongmei Zhang, Jie Liu, Qisheng Feng, Caixia Wu and Zhiwei Wang
    Citation: Plant Methods 2023 19:48
  33. Climate change due to different human activities is causing adverse environmental conditions and uncontrolled extreme weather events. These harsh conditions are directly affecting the crop areas, and consequen...

    Authors: Jimmy Sampedro-Guerrero, Vicente Vives-Peris, Aurelio Gomez-Cadenas and Carolina Clausell-Terol
    Citation: Plant Methods 2023 19:47
  34. Detecting and counting wheat spikes is essential for predicting and measuring wheat yield. However, current wheat spike detection researches often directly apply the new network structure. There are few studie...

    Authors: Jiawei Yan, Jianqing Zhao, Yucheng Cai, Suwan Wang, Xiaolei Qiu, Xia Yao, Yongchao Tian, Yan Zhu, Weixing Cao and Xiaohu Zhang
    Citation: Plant Methods 2023 19:46
  35. The Mg–Al-lactate layered double hydroxide nanosheet (LDH-NS) has shown great potential as an optimal nanocarrier for extensive use in plants. However, previous studies in plant sciences have not provided a cl...

    Authors: He Zhang, Xinyu Li, Dong Yu, Junqi Guan, Hao Ding, Hongyang Wu, Qiang Wang and Yinglang Wan
    Citation: Plant Methods 2023 19:44
  36. The current development of sensor technologies towards ever more cost-effective and powerful systems is steadily increasing the application of low-cost sensors in different horticultural sectors. In plant in v...

    Authors: Hans Bethge, Traud Winkelmann, Patrick Lüdeke and Thomas Rath
    Citation: Plant Methods 2023 19:42

    The Correction to this article has been published in Plant Methods 2023 19:133

  37. PacBio HiFi sequencing provides highly accurate long-read sequencing datasets which are of great advantage for whole genome sequencing projects. One limitation of the method is the requirement for high quality...

    Authors: Kanae Nishii, Michael Möller, Robert G. Foster, Laura L. Forrest, Nathan Kelso, Sadie Barber, Caroline Howard and Michelle L. Hart
    Citation: Plant Methods 2023 19:41
  38. The flowering period is a critical time for the growth of rape plants. Counting rape flower clusters can help farmers to predict the yield information of the corresponding rape fields. However, counting in-fie...

    Authors: Jie Li, Enguo Wang, Jiangwei Qiao, Yi Li, Li Li, Jian Yao and Guisheng Liao
    Citation: Plant Methods 2023 19:40
  39. Canopy height (CH) is an important trait for crop breeding and production. The rapid development of 3D sensing technologies shed new light on high-throughput height measurement. However, a systematic compariso...

    Authors: Jingrong Zang, Shichao Jin, Songyin Zhang, Qing Li, Yue Mu, Ziyu Li, Shaochen Li, Xiao Wang, Yanjun Su and Dong Jiang
    Citation: Plant Methods 2023 19:39

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