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  1. Research

    Developing transgenic wheat to encounter rusts and powdery mildew by overexpressing barley chi26 gene for fungal resistance

    The main aim of this study was to improve fungal resistance in bread wheat via transgenesis. Transgenic wheat plants harboring barley chitinase (chi26) gene, driven by maize ubi promoter, were obtained using biol...

    Hala F. Eissa, Sameh E. Hassanien, Ahmed M. Ramadan, Moustafa M. El-Shamy, Osama M. Saleh, Ahmed M. Shokry, Mohamed Abdelsattar, Yasser B. Morsy, Maher A. El-Maghraby, Hussien F. Alameldin, Sabah M. Hassan, Gamal H. Osman, Hesham T. Mahfouz, Gharib A. Gad El-Karim, Magdy A. Madkour and Ahmed Bahieldin

    Plant Methods 2017 13:41

    Published on: 22 May 2017

  2. Methodology

    A method to estimate plant density and plant spacing heterogeneity: application to wheat crops

    Plant density and its non-uniformity drive the competition among plants as well as with weeds. They need thus to be estimated with small uncertainties accuracy. An optimal sampling method is proposed to estima...

    Shouyang Liu, Fred Baret, Denis Allard, Xiuliang Jin, Bruno Andrieu, Philippe Burger, Matthieu Hemmerlé and Alexis Comar

    Plant Methods 2017 13:38

    Published on: 17 May 2017

  3. Methodology

    Building a multipurpose insertional mutant library for forward and reverse genetics in Chlamydomonas

    The unicellular green alga, Chlamydomonas reinhardtii, is a classic model for studying flagella and biofuel. However, precise gene editing, such as Clustered Regularly Interspaced Short Palindromic Repeats (CRISP...

    Xi Cheng, Gai Liu, Wenting Ke, Lijuan Zhao, Bo Lv, Xiaocui Ma, Nannan Xu, Xiaoling Xia, Xuan Deng, Chunlei Zheng and Kaiyao Huang

    Plant Methods 2017 13:36

    Published on: 15 May 2017

  4. Methodology

    In situ measurement of Scots pine needle PRI

    The Photochemical Reflectance Index (PRI) calculated from narrow-band spectral reflectance data is a vegetation index which is increasingly used as an indicator of photosynthetic activity. The leaf-level link ...

    Matti Mõttus, Rocío Hernández-Clemente, Viljami Perheentupa and Vincent Markiet

    Plant Methods 2017 13:35

    Published on: 10 May 2017

  5. Methodology

    Development of a new heat tolerance assay system for rice spikelet sterility

    Reduction in rice yield caused by high temperature-induced spikelet sterility has been a serious concern in rice production. To date, several screening methods have been used, although their reproducibility i...

    Makoto Hakata, Hiroshi Wada, Chisato Masumoto-Kubo, Ryo Tanaka, Hiroyuki Sato and Satoshi Morita

    Plant Methods 2017 13:34

    Published on: 10 May 2017

  6. Methodology

    Microscopy of Physcomitrella patens sperm cells

    Archegoniates (bryophytes, ferns and gymnosperms), such as the moss Physcomitrella patens, possess freely motile sperm cells (spermatozoids) which reach the egg cell via surface water. Although these motile flage...

    Nelly A. Horst and Ralf Reski

    Plant Methods 2017 13:33

    Published on: 10 May 2017

  7. Methodology

    A novel hairpin library-based approach to identify NBS–LRR genes required for effector-triggered hypersensitive response in Nicotiana benthamiana

    PTI and ETI are the two major defence mechanisms in plants. ETI is triggered by the detection of pathogen effectors, or their activity, in the plant cell and most of the time involves internal receptors known ...

    Cyril Brendolise, Mirco Montefiori, Romain Dinis, Nemo Peeters, Roy D. Storey and Erik H. Rikkerink

    Plant Methods 2017 13:32

    Published on: 28 April 2017

  8. Review

    Recent achievements obtained by chloroplast transformation

    Chloroplasts play a great role for sustained wellbeing of life on the planet. They have the power and raw materials that can be used as sophisticated biological factories. They are rich in energy as they have ...

    Muhamed Adem, Dereje Beyene and Tileye Feyissa

    Plant Methods 2017 13:30

    Published on: 19 April 2017

  9. Methodology

    Reference genes for normalization of qPCR assays in sugarcane plants under water deficit

    Sugarcane (Saccharum spp.) is the main raw material for sugar and ethanol production. Among the abiotic stress, drought is the main one that negatively impact sugarcane yield. Although gene expression analysis th...

    Larissa Mara de Andrade, Michael dos Santos Brito, Rafael Fávero Peixoto Junior, Paulo Eduardo Ribeiro Marchiori, Paula Macedo Nóbile, Alexandre Palma Boer Martins, Rafael Vasconcelos Ribeiro and Silvana Creste

    Plant Methods 2017 13:28

    Published on: 17 April 2017

  10. Methodology

    Protoplast isolation prior to flow cytometry reveals clear patterns of endoreduplication in potato tubers, related species, and some starchy root crops

    Endoreduplication, the process of DNA replication in the absence of cell division, is associated with specialized cellular function and increased cell size. Genes controlling endoreduplication in tomato fruit ...

    F. Parker E. Laimbeer, Sarah H. Holt, Melissa Makris, Michael Alan Hardigan, C. Robin Buell and Richard E. Veilleux

    Plant Methods 2017 13:27

    Published on: 14 April 2017

  11. Methodology

    Ex vitro hairy root induction in detached peanut leaves for plant–nematode interaction studies

    Peanut (Arachis hypogaea) production is largely affected by a variety of abiotic and biotic stresses, including the root-knot nematode (RKN) Meloidogyne arenaria that causes yield losses worldwide. Transcriptome ...

    Larissa Arrais Guimaraes, Bruna Medeiros Pereira, Ana Claudia Guerra Araujo, Patricia Messenberg Guimaraes and Ana Cristina Miranda Brasileiro

    Plant Methods 2017 13:25

    Published on: 11 April 2017

  12. Methodology

    A real-time phenotyping framework using machine learning for plant stress severity rating in soybean

    Phenotyping is a critical component of plant research. Accurate and precise trait collection, when integrated with genetic tools, can greatly accelerate the rate of genetic gain in crop improvement. However, e...

    Hsiang Sing Naik, Jiaoping Zhang, Alec Lofquist, Teshale Assefa, Soumik Sarkar, David Ackerman, Arti Singh, Asheesh K. Singh and Baskar Ganapathysubramanian

    Plant Methods 2017 13:23

    Published on: 8 April 2017

  13. Methodology

    A robust protocol for efficient generation, and genomic characterization of insertional mutants of Chlamydomonas reinhardtii

    Random insertional mutagenesis of Chlamydomonas reinhardtii using drug resistance cassettes has contributed to the generation of tens of thousands of transformants in dozens of labs around the world. In many inst...

    Steve V. Pollock, Bratati Mukherjee, Joanna Bajsa-Hirschel, Marylou C. Machingura, Ananya Mukherjee, Arthur R. Grossman and James V. Moroney

    Plant Methods 2017 13:22

    Published on: 3 April 2017

  14. Methodology

    TIPS: a system for automated image-based phenotyping of maize tassels

    The maize male inflorescence (tassel) produces pollen necessary for reproduction and commercial grain production of maize. The size of the tassel has been linked to factors affecting grain yield, so understand...

    Joseph L. Gage, Nathan D. Miller, Edgar P. Spalding, Shawn M. Kaeppler and Natalia de Leon

    Plant Methods 2017 13:21

    Published on: 31 March 2017

  15. Methodology

    Growth curve registration for evaluating salinity tolerance in barley

    Smarthouses capable of non-destructive, high-throughput plant phenotyping collect large amounts of data that can be used to understand plant growth and productivity in extreme environments. The challenge is to...

    Rui Meng, Stephanie Saade, Sebastian Kurtek, Bettina Berger, Chris Brien, Klaus Pillen, Mark Tester and Ying Sun

    Plant Methods 2017 13:18

    Published on: 23 March 2017

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