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  1. Research

    A plant-based chemical genomics screen for the identification of flowering inducers

    Floral timing is a carefully regulated process, in which the plant determines the optimal moment to switch from the vegetative to reproductive phase. While there are numerous genes known that control flowering...

    Martijn Fiers, Jorin Hoogenboom, Alice Brunazzi, Tom Wennekes, Gerco C. Angenent and Richard G. H. Immink

    Plant Methods 2017 13:78

    Published on: 3 October 2017

  2. Methodology article

    An optimized method for high quality DNA extraction from microalga Prototheca wickerhamii for genome sequencing

    The complex cell wall structure of algae often precludes efficient extraction of their genetic material. The purpose of this study was to design a next-generation sequencing-suitable DNA isolation method for u...

    Tomasz Jagielski, Jan Gawor, Zofia Bakuła, Karolina Zuchniewicz, Iwona Żak and Robert Gromadka

    Plant Methods 2017 13:77

    Published on: 3 October 2017

  3. Research

    A dual isotopic approach using radioactive phosphorus and the isotopic composition of oxygen associated to phosphorus to understand plant reaction to a change in P nutrition

    Changing the phosphorus (P) nutrition leads to changes in plant metabolism. The aim of this study was to investigate how these changes are reflected in the distribution of 33P and the isotopic composition of oxy...

    Verena Pfahler, Federica Tamburini, Stefano M. Bernasconi and Emmanuel Frossard

    Plant Methods 2017 13:75

    Published on: 25 September 2017

  4. Research

    Comparison of visible imaging, thermography and spectrometry methods to evaluate the effect of Heterodera schachtii inoculation on sugar beets

    Phenotyping technologies are expected to provide predictive power for a range of applications in plant and crop sciences. Here, we use the disease pressure of Beet Cyst Nematodes (BCN) on sugar beet as an illu...

    Samuel Joalland, Claudio Screpanti, Frank Liebisch, Hubert Vincent Varella, Alain Gaume and Achim Walter

    Plant Methods 2017 13:73

    Published on: 13 September 2017

  5. Methodology article

    Development of TaqMan probes targeting the four major celiac disease epitopes found in α-gliadin sequences of spelt (Triticum aestivum ssp. spelta) and bread wheat (Triticum aestivum ssp. aestivum)

    Celiac disease (CD) is caused by specific sequences of gluten proteins found in cereals such as bread wheat (Triticum aestivum ssp. aestivum) and spelt (T. aestivum ssp. spelta). Among them, the α-gliadins displa...

    Benjamin Dubois, Pierre Bertin, Yordan Muhovski, Emmanuelle Escarnot and Dominique Mingeot

    Plant Methods 2017 13:72

    Published on: 6 September 2017

  6. Methodology article

    Quantification of seed–soil contact of sugar beet (Beta vulgaris) using X-ray Computed Tomography

    Seed–soil contact is important to ensure successful germination, however, there is a paucity of reported studies that have quantified the microstructure at and around this critical interface, mainly due to the...

    Sebastian Blunk, Ali Hafeez Malik, Martine I. de Heer, Tobias Ekblad, Jennifer Bussell, Debbie Sparkes, Kenneth Fredlund, Craig J. Sturrock and Sacha J. Mooney

    Plant Methods 2017 13:71

    Published on: 30 August 2017

  7. Research

    Discrimination of boron tolerance in Pisum sativum L. genotypes using a rapid, high-throughput hydroponic screen and precociously germinated seed grown under far-red enriched light

    Boron (B) tolerance has been identified as a key target for field pea improvement. Screening for B tolerance in the field is problematic due to variability in space and time, and robust B molecular markers are...

    Richard G. Bennett, Federico M. Ribalta, Maria Pazos-Navarro, Antonio Leonforte and Janine S. Croser

    Plant Methods 2017 13:70

    Published on: 29 August 2017

  8. Methodology article

    Kinetic modelling: an integrated approach to analyze enzyme activity assays

    In general, enzyme activity is estimated from spectrophotometric data, by taking the slope of the linear part of the progress curve describing the rate of change in the substrate or product monitored. As long ...

    Jelena Boeckx, Maarten Hertog, Annemie Geeraerd and Bart Nicolai

    Plant Methods 2017 13:69

    Published on: 25 August 2017

  9. Research

    Ground penetrating radar: a case study for estimating root bulking rate in cassava (Manihot esculenta Crantz)

    Understanding root traits is a necessary research front for selection of favorable genotypes or cultivation practices. Root and tuber crops having most of their economic potential stored below ground are favor...

    Alfredo Delgado, Dirk B. Hays, Richard K. Bruton, Hernán Ceballos, Alexandre Novo, Enrico Boi and Michael Gomez Selvaraj

    Plant Methods 2017 13:65

    Published on: 7 August 2017

  10. Research

    Quantitation of glutathione S-transferases in rice (Oryza sativa L.) roots exposed to cadmium by liquid chromatography–tandem mass spectrometry using isotope-labeled wing peptides as an internal standard

    Plant glutathione S-transferases (GSTs, EC are multifunctional enzymes involved in heavy metal cellular detoxification by conjugating the tripeptide (g-Glu-Cys-Gly) glutathione to heavy metals. Previous...

    Zhenzhen Cao, Renxiang Mou, Zhaoyun Cao, Xiaoyan Lin, Youning Ma, Zhiwei Zhu and Mingxue Chen

    Plant Methods 2017 13:64

    Published on: 4 August 2017

  11. Methodology article

    An improved butanol-HCl assay for quantification of water-soluble, acetone:methanol-soluble, and insoluble proanthocyanidins (condensed tannins)

    Condensed tannins (CT) are the most abundant secondary metabolite of land plants and can vary in abundance and structure according to tissue type, species, genotype, age, and environmental conditions. Recent i...

    Philip-Edouard Shay, J. A. Trofymow and C. Peter Constabel

    Plant Methods 2017 13:63

    Published on: 2 August 2017

  12. Methodology article

    Genomic Bayesian functional regression models with interactions for predicting wheat grain yield using hyper-spectral image data

    Modern agriculture uses hyperspectral cameras that provide hundreds of reflectance data at discrete narrow bands in many environments. These bands often cover the whole visible light spectrum and part of the i...

    Abelardo Montesinos-López, Osval A. Montesinos-López, Jaime Cuevas, Walter A. Mata-López, Juan Burgueño, Sushismita Mondal, Julio Huerta, Ravi Singh, Enrique Autrique, Lorena González-Pérez and José Crossa

    Plant Methods 2017 13:62

    Published on: 27 July 2017

  13. Methodology article

    A procedure for maize genotypes discrimination to drought by chlorophyll fluorescence imaging rapid light curves

    Photosynthesis can be roughly separated into biochemical and photochemical processes. Both are affected by drought and can be assessed by non-invasive standard methods. Gas exchange, which mainly assesses the ...

    Carlos Antônio Ferreira de Sousa, Dayane Silva de Paiva, Raphael Augusto das Chagas Noqueli Casari, Nelson Geraldo de Oliveira, Hugo Bruno Correa Molinari, Adilson Kenji Kobayashi, Paulo Cesar Magalhães, Reinaldo Lúcio Gomide and Manoel Teixeira Souza Jr.

    Plant Methods 2017 13:61

    Published on: 26 July 2017

  14. Methodology article

    Elucidating the role of highly homologous Nicotiana benthamiana ubiquitin E2 gene family members in plant immunity through an improved virus-induced gene silencing approach

    Virus-induced gene silencing (VIGS) has been used in many plant species as an attractive post transcriptional gene silencing (PTGS) method for studying gene function either individually or at large-scale in a ...

    Bangjun Zhou and Lirong Zeng

    Plant Methods 2017 13:59

    Published on: 21 July 2017

  15. Methodology

    Accelerating root system phenotyping of seedlings through a computer-assisted processing pipeline

    There are numerous systems and techniques to measure the growth of plant roots. However, phenotyping large numbers of plant roots for breeding and genetic analyses remains challenging. One major difficulty is ...

    Lionel X. Dupuy, Gladys Wright, Jacqueline A. Thompson, Anna Taylor, Sebastien Dekeyser, Christopher P. White, William T. B. Thomas, Mark Nightingale, John P. Hammond, Neil S. Graham, Catherine L. Thomas, Martin R. Broadley and Philip J. White

    Plant Methods 2017 13:57

    Published on: 13 July 2017

  16. Methodology

    Development of a phenotyping platform for high throughput screening of nodal root angle in sorghum

    In sorghum, the growth angle of nodal roots is a major component of root system architecture. It strongly influences the spatial distribution of roots of mature plants in the soil profile, which can impact dro...

    Dinesh C. Joshi, Vijaya Singh, Colleen Hunt, Emma Mace, Erik van Oosterom, Richard Sulman, David Jordan and Graeme Hammer

    Plant Methods 2017 13:56

    Published on: 11 July 2017

  17. Methodology article

    Quantifying pruning impacts on olive tree architecture and annual canopy growth by using UAV-based 3D modelling

    Tree pruning is a costly practice with important implications for crop harvest and nutrition, pest and disease control, soil protection and irrigation strategies. Investigations on tree pruning usually involve...

    F. M. Jiménez-Brenes, F. López-Granados, A. I. de Castro, J. Torres-Sánchez, N. Serrano and J. M. Peña

    Plant Methods 2017 13:55

    Published on: 6 July 2017

  18. Research

    A mobile NMR lab for leaf phenotyping in the field

    Low field NMR has been used to investigate water status in various plant tissues. In plants grown in controlled conditions, the method was shown to be able to monitor leaf development as it could detect slight...

    Maja Musse, Laurent Leport, Mireille Cambert, William Debrandt, Clément Sorin, Alain Bouchereau and François Mariette

    Plant Methods 2017 13:53

    Published on: 28 June 2017

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