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  1. Content type: Research

    Gene editing using CRISPR/Cas9 is a simple and powerful tool for elucidating genetic controls and for crop improvement and its use has been reported in a growing number of important food crops, including recen...

    Authors: Fiona M. Wilson, Kate Harrison, Andrew D. Armitage, Andrew J. Simkin and Richard J. Harrison

    Citation: Plant Methods 2019 15:45

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  2. Content type: Methodology

    With the introduction of high-throughput multisensory imaging platforms, the automatization of multimodal image analysis has become the focus of quantitative plant research. Due to a number of natural and tech...

    Authors: Michael Henke, Astrid Junker, Kerstin Neumann, Thomas Altmann and Evgeny Gladilin

    Citation: Plant Methods 2019 15:44

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  3. Content type: Research

    Efficient seed germination is a crucial task at the beginning of crop cultivation. Although boundaries of environmental parameters that should be maintained are well studied, fine-tuning can significantly impr...

    Authors: Artyom Nikitin, Ilia Fastovets, Dmitrii Shadrin, Mariia Pukalchik and Ivan Oseledets

    Citation: Plant Methods 2019 15:43

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  4. Content type: Methodology

    Many agronomic traits have been bred into modern wheat varieties, but wheat (Triticum aestivum L.) continues to be vulnerable to heat stress, with high night-time temperature (HNT) stress shown to have large nega...

    Authors: Nathan T. Hein, Dan Wagner, Raju Bheemanahalli, David Šebela, Carlos Bustamante, Anuj Chiluwal, Mitchell L. Neilsen and S. V. Krishna Jagadish

    Citation: Plant Methods 2019 15:41

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  5. Content type: Methodology

    Glandular trichomes found in vascular plants are called natural cell factories because they synthesize and store secondary metabolites in glandular cells. To systematically understand the metabolic processes i...

    Authors: Ayako Takemori, Taiken Nakashima, Hisashi Ômura, Yuki Tanaka, Keisuke Nakata, Hiroshi Nonami and Nobuaki Takemori

    Citation: Plant Methods 2019 15:40

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  6. Content type: Research

    Guard cells perceive external and internal stimuli and regulate stomatal conductance in plants. With the use of gas exchange analyzers, time-resolved stomatal conductance responses to light intensity, [CO2] conce...

    Authors: Paulo H. O. Ceciliato, Jingbo Zhang, Qing Liu, Xin Shen, Honghong Hu, Chen Liu, Anton R. Schäffner and Julian I. Schroeder

    Citation: Plant Methods 2019 15:38

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  7. Content type: Research

    Plant height is an important selection target since it is associated with yield potential, stability and particularly with lodging resistance in various environments. Rapid and cost-effective estimation of pla...

    Authors: Muhammad Adeel Hassan, Mengjiao Yang, Luping Fu, Awais Rasheed, Bangyou Zheng, Xianchun Xia, Yonggui Xiao and Zhonghu He

    Citation: Plant Methods 2019 15:37

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  8. Content type: Research

    As strawberries are susceptible to somaclonal variation when propagated by tissue culture techniques, it is challenging to obtain the true-to-type plants necessary for continuous production of fruits of stable...

    Authors: Aung Htay Naing, Si Hyun Kim, Mi Young Chung, Soon Ki Park and Chang Kil Kim

    Citation: Plant Methods 2019 15:36

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  9. Content type: Research

    Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) is a rapid and sensitive approach to identify miRNA and protein-coding gene expression in plants. However, because of the specially design...

    Authors: Fang Tang, Liwei Chu, Wenbo Shu, Xuejiao He, Lijuan Wang and Mengzhu Lu

    Citation: Plant Methods 2019 15:35

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  10. Content type: Methodology

    Modern imaging approaches enable the acquisition of 3D and 4D datasets capturing plant organ development at cellular resolution. Computational analyses of these data enable the digitization and analysis of ind...

    Authors: Thomas Montenegro-Johnson, Soeren Strauss, Matthew D. B. Jackson, Liam Walker, Richard S. Smith and George W. Bassel

    Citation: Plant Methods 2019 15:33

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  11. Content type: Research

    Unmanned aerial vehicle (UAV)-based remote sensing provides a flexible, low-cost, and efficient approach to monitor crop growth status at fine spatial and temporal resolutions, and has a high potential to acce...

    Authors: Haiyan Cen, Liang Wan, Jiangpeng Zhu, Yijian Li, Xiaoran Li, Yueming Zhu, Haiyong Weng, Weikang Wu, Wenxin Yin, Chi Xu, Yidan Bao, Lei Feng, Jianyao Shou and Yong He

    Citation: Plant Methods 2019 15:32

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  12. Content type: Research

    Reverse transcription quantitative real-time PCR (RT-qPCR) is a widely used approach for investigating gene expression levels in plants because of its high reproducibility, sensitivity, accuracy and rapidness....

    Authors: Huapeng Sun, Xuefei Jiang, Mengli Sun, Hanqing Cong and Fei Qiao

    Citation: Plant Methods 2019 15:31

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  13. Content type: Research

    Rice canopy changes are associated with changes in the red light (R), green light (G), and blue light (B) value parameters of digital images. To rapidly diagnose the responses of rice to nitrogen (N) fertilize...

    Authors: Kailou Liu, Yazhen Li, Tianfu Han, Xichu Yu, Huicai Ye, Huiwen Hu and Zhihua Hu

    Citation: Plant Methods 2019 15:28

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  14. Content type: Methodology

    Roots are vital organs for plants, and the effective use of resources from the soil is important for yield stability. However, phenotypic variation in root traits among crop genotypes is mostly unknown and fie...

    Authors: Simon Fiil Svane, Christian Sig Jensen and Kristian Thorup-Kristensen

    Citation: Plant Methods 2019 15:26

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  15. Content type: Methodology

    We have recently developed a simple, rapid, and relatively-cheap method for matrix-assisted laser-desorption and ionisation time-of-flight mass spectroscopy (MALDI-TOF MS) sample preparation that is applicable...

    Authors: Michael A. Reeve and Kathryn M. Pollard

    Citation: Plant Methods 2019 15:25

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  16. Content type: Research

    The traditional methods for evaluating seeds are usually performed through destructive sampling followed by physical, physiological, biochemical and molecular determinations. Whilst proven to be effective, the...

    Authors: Gamal ElMasry, Nasser Mandour, Marie-Hélène Wagner, Didier Demilly, Jerome Verdier, Etienne Belin and David Rousseau

    Citation: Plant Methods 2019 15:24

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  17. Content type: Methodology

    Nowadays, there is an increasing demand for fast and reliable plant biomolecular analyses. Conventional methods for the isolation of nucleic acids are time-consuming and require multiple and often non-automata...

    Authors: Arianna Marengo, Cecilia Cagliero, Barbara Sgorbini, Jared L. Anderson, Miranda N. Emaus, Carlo Bicchi, Cinzia M. Bertea and Patrizia Rubiolo

    Citation: Plant Methods 2019 15:23

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  18. Content type: Methodology

    In the present work, we provide an account of structured illumination microscopy (SIM) imaging of fixed and immunolabeled plant probes. We take advantage of SIM, to superresolve intracellular structures at a c...

    Authors: T. Vavrdová, O. Šamajová, P. Křenek, M. Ovečka, P. Floková, R. Šnaurová, J. Šamaj and G. Komis

    Citation: Plant Methods 2019 15:22

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  19. Content type: Methodology

    Tissue culture transformation of plants has an element of art to it, with protocols passed on between labs but often not directly compared. As Medicago truncatula has become popular as a model system for legumes,...

    Authors: Li Wen, Yuanling Chen, Elise Schnabel, Ashley Crook and Julia Frugoli

    Citation: Plant Methods 2019 15:20

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  20. Content type: Methodology

    Chlorophyll fluorescence analysis is one of the non-invasive techniques widely used to detect and quantify the stress-induced changes in the photosynthetic apparatus. Quantitative information is obtained as a ...

    Authors: Joanna Sekulska-Nalewajko, Andrzej Kornaś, Jarosław Gocławski, Zbigniew Miszalski and Elżbieta Kuźniak

    Citation: Plant Methods 2019 15:18

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  21. Content type: Research

    Aboveground biomass (AGB) is a widely used agronomic parameter for characterizing crop growth status and predicting grain yield. The rapid and accurate estimation of AGB in a non-destructive way is useful for ...

    Authors: Ning Lu, Jie Zhou, Zixu Han, Dong Li, Qiang Cao, Xia Yao, Yongchao Tian, Yan Zhu, Weixing Cao and Tao Cheng

    Citation: Plant Methods 2019 15:17

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  22. Content type: Methodology

    Plant responses triggered upon detection of an invading pathogen include the generation of a number of mobile signals that travel to distant tissues and determine an increased resistance in distal, uninfected ...

    Authors: José S. Rufián, Javier Rueda-Blanco, Carmen R. Beuzón and Javier Ruiz-Albert

    Citation: Plant Methods 2019 15:16

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  23. Content type: Research

    Crop emergence and canopy cover are important physiological traits for potato (Solanum tuberosum L.) cultivar evaluation and nutrients management. They play important roles in variety screening, field management ...

    Authors: Bo Li, Xiangming Xu, Jiwan Han, Li Zhang, Chunsong Bian, Liping Jin and Jiangang Liu

    Citation: Plant Methods 2019 15:15

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  24. Content type: Research

    The selection of hybrids is an essential step in maize breeding. However, evaluating a large number of hybrids in field trials can be extremely costly. However, genomic models can be used to predict the expect...

    Authors: Filipe Couto Alves, Ítalo Stefanine Correa Granato, Giovanni Galli, Danilo Hottis Lyra, Roberto Fritsche-Neto and Gustavo de los Campos

    Citation: Plant Methods 2019 15:14

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  25. Content type: Methodology

    Recent advances in high throughput phenotyping have made it possible to collect large datasets following plant growth and development over time, and those in machine learning have made inferring phenotypic pla...

    Authors: Raghav Khanna, Lukas Schmid, Achim Walter, Juan Nieto, Roland Siegwart and Frank Liebisch

    Citation: Plant Methods 2019 15:13

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  26. Content type: Research

    Maize (Zea mays L.) is the third most consumed grain in the world and improving maize yield is of great importance of the world food security, especially under global climate change and more frequent severe droug...

    Authors: Yanjun Su, Fangfang Wu, Zurui Ao, Shichao Jin, Feng Qin, Boxin Liu, Shuxin Pang, Lingli Liu and Qinghua Guo

    Citation: Plant Methods 2019 15:11

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  27. Content type: Research

    Above-ground biomass (AGB) is a basic agronomic parameter for field investigation and is frequently used to indicate crop growth status, the effects of agricultural management practices, and the ability to seq...

    Authors: Liang Han, Guijun Yang, Huayang Dai, Bo Xu, Hao Yang, Haikuan Feng, Zhenhai Li and Xiaodong Yang

    Citation: Plant Methods 2019 15:10

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  28. Content type: Research

    Bacterial volatiles promote plant growth and elicit immunity responses in plants grown in two-compartment Petri dishes. Due to the limitations of bacterial volatile compound (BVC) treatments such as their high...

    Authors: Geun Cheol Song, Myoungjoo Riu and Choong-Min Ryu

    Citation: Plant Methods 2019 15:9

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  29. Content type: Research

    Plant root apex is the major part to direct the root growth and development by responding to various signals/cues from internal and soil environments. To study and understand root system biology particularly a...

    Authors: Lei Zhang, Li-Na Qin, Zi-Rui Zeng, Chang-Zheng Wu, Yuan-Yong Gong, Lai-Hua Liu and Feng-Qiu Cao

    Citation: Plant Methods 2019 15:8

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  30. Content type: Research

    Loquat (Eriobotrya japonica) is a subtropical tree bearing fruit that ripens during late spring and early summer, which is the off-season for fruit production. The specific flowering habit of loquat, which starts...

    Authors: Wenbing Su, Yuan Yuan, Ling Zhang, Yuanyuan Jiang, Xiaoqing Gan, Yunlu Bai, Jiangrong Peng, Jincheng Wu, Yuexue Liu and Shunquan Lin

    Citation: Plant Methods 2019 15:7

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  31. Content type: Methodology

    Remote monitoring of plants using hyperspectral imaging has become an important tool for the study of plant growth, development, and physiology. Many applications are oriented towards use in field environments...

    Authors: Max R. Lien, Richard J. Barker, Zhiwei Ye, Matthew H. Westphall, Ruohan Gao, Aditya Singh, Simon Gilroy and Philip A. Townsend

    Citation: Plant Methods 2019 15:6

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  32. Content type: Research

    Open tissue culture technique could be simplified by using different bacteriostatic agents. There is a great difference in the bacteriostatic effects of different antimicrobial agents on various explants. Howe...

    Authors: Liejian Huang, Hong Wang, Muhammad Qasim Shahid and Bingshan Zeng

    Citation: Plant Methods 2019 15:5

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  33. Content type: Methodology

    The use of spectral imaging within the plant phenotyping and breeding community has been increasing due its utility as a non-invasive diagnostic tool. However, there is a lack of imaging systems targeted speci...

    Authors: Charles Veys, Fokion Chatziavgerinos, Ali AlSuwaidi, James Hibbert, Mark Hansen, Gytis Bernotas, Melvyn Smith, Hujun Yin, Stephen Rolfe and Bruce Grieve

    Citation: Plant Methods 2019 15:4

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  34. Content type: Methodology

    Global warming is going to affect both agricultural production and carbon storage in soil worldwide. Given the complexity of the soil-plant-atmosphere continuum, in situ experiments of climate warming are nece...

    Authors: Christophe Moni, Hanna Silvennoinen, Bruce A. Kimball, Erling Fjelldal, Marius Brenden, Ingunn Burud, Andreas Flø and Daniel P. Rasse

    Citation: Plant Methods 2019 15:3

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  35. Content type: Software

    The development of leaf area is one of the fundamental variables to quantify plant growth and physiological function and is therefore widely used to characterize genotypes and their interaction with the enviro...

    Authors: Mark Müller-Linow, Jens Wilhelm, Christoph Briese, Tobias Wojciechowski, Ulrich Schurr and Fabio Fiorani

    Citation: Plant Methods 2019 15:2

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  36. Content type: Methodology

    Cutin is a complex, highly cross-linked polyester consisting of hydroxylated and epoxidated acyl lipid monomers. Because of the complexity of the polymer it has been difficult to define the chemical architectu...

    Authors: Rupam Kumar Bhunia, Lucas J. Showman, Adarsh Jose and Basil J. Nikolau

    Citation: Plant Methods 2018 14:117

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  37. Content type: Research

    Imbibition (uptake of water by a dry seed) initiates the germination process. An automated method for quantifying imbibition would enable research on the genetic elements that influence the underlying hydrauli...

    Authors: Nathan D. Miller, Scott C. Stelpflug, Shawn M. Kaeppler and Edgar P. Spalding

    Citation: Plant Methods 2018 14:115

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  38. Content type: Methodology

    Plant roots release a variety of organic compounds into the soil which alter the physical, chemical and biological properties of the rhizosphere. Root exudates are technically challenging to measure in soil be...

    Authors: Akitomo Kawasaki, Shoko Okada, Chunyan Zhang, Emmanuel Delhaize, Ulrike Mathesius, Alan E. Richardson, Michelle Watt, Matthew Gilliham and Peter R. Ryan

    Citation: Plant Methods 2018 14:114

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  39. Content type: Research

    There is a growing interest in the role of chromatin in acquiring and maintaining cell identity. Despite the ever-growing availability of genome-wide gene expression data, understanding how transcription progr...

    Authors: Miriam Tannenbaum, Avital Sarusi-Portuguez, Ronen Krispil, Michal Schwartz, Olga Loza, Jennifer I. C. Benichou, Assaf Mosquna and Ofir Hakim

    Citation: Plant Methods 2018 14:113

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