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  1. We report a method to estimate carbon assimilation based on isotope ratio-mass spectrometry (IRMS) of 13CO2 labeled plant tissue. Photosynthetic carbon assimilation is the principal experimental observable which ...

    Authors: Matthew E. Bergman, Diego González-Cabanelas, Louwrance P. Wright, Berkley J. Walker and Michael A. Phillips

    Citation: Plant Methods 2021 17:32

    Content type: Methodology

    Published on:

  2. In vitro culture of olive, as an economically valuable tree, has fundamentally a genotype-dependant low micropropagation rate which needs to be improved in already established and newly released cultivars. Var...

    Authors: Leila Mirzaei, Abbas Yadollahi, Maryam Jafarkhani Kermani, Masoud Naderpour, Ali Asghar Zeinanloo, Maryam Farsi and Dariush Davoodi

    Citation: Plant Methods 2021 17:31

    Content type: Research

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  3. K-seq, a new genotyping methodology based on the amplification of genomic regions using two steps of Klenow amplification with short oligonucleotides, followed by standard PCR and Illumina sequencing, is prese...

    Authors: Peio Ziarsolo, Tomas Hasing, Rebeca Hilario, Victor Garcia-Carpintero, Jose Blanca, Aureliano Bombarely and Joaquin Cañizares

    Citation: Plant Methods 2021 17:30

    Content type: Methodology

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  4. New cell wall imaging tools permit direct visualization of the molecular architecture of cell walls and provide detailed chemical information on wall polymers, which will aid efforts to use these polymers in m...

    Authors: Huimin Xu, Yuanyuan Zhao, Yuanzhen Suo, Yayu Guo, Yi Man, Yanping Jing, Xinqiang He and Jinxing Lin

    Citation: Plant Methods 2021 17:29

    Content type: Methodology

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  5. Wheat spike architecture is a key determinant of multiple grain yield components and detailed examination of spike morphometric traits is beneficial to explain wheat grain yield and the effects of differing ag...

    Authors: Hu Zhou, Andrew B. Riche, Malcolm J. Hawkesford, William R. Whalley, Brian S. Atkinson, Craig J. Sturrock and Sacha J. Mooney

    Citation: Plant Methods 2021 17:26

    Content type: Methodology

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  6. Floral temperature has important consequences for plant biology, and accurate temperature measurements are therefore important to plant research. Thermography, also referred to as thermal imaging, is beginning...

    Authors: Michael J. M. Harrap and Sean A. Rands

    Citation: Plant Methods 2021 17:23

    Content type: Research

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  7. The virus-induced genome editing (VIGE) system can be used to quickly identify gene functions and generate knock-out libraries as an alternative to the virus-induced gene silencing (VIGS). Although plant virus...

    Authors: Jianfeng Lei, Peihong Dai, Yue Li, Wanqi Zhang, Guantong Zhou, Chao Liu and Xiaodong Liu

    Citation: Plant Methods 2021 17:20

    Content type: Methodology

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  8. Dispensability of genes in a phylogenetic lineage, e.g. a species, genus, or higher-level clade, is gaining relevance as most genome sequencing projects move to a pangenome level. Most analyses classify genes ...

    Authors: Katharina Sielemann, Bernd Weisshaar and Boas Pucker

    Citation: Plant Methods 2021 17:18

    Content type: Methodology

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  9. Upland cotton (Gossypium hirsutum), harboring a complex allotetraploid genome, consists of A and D sub-genomes. Every gene has multiple copies with high sequence similarity that makes genetic, genomic and functio...

    Authors: Mohamed Ramadan, Muna Alariqi, Yizan Ma, Yanlong Li, Zhenping Liu, Rui Zhang, Shuangxia Jin, Ling Min and Xianlong Zhang

    Citation: Plant Methods 2021 17:16

    Content type: Methodology

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  10. Casbene synthase (CS) is responsible for the first committed step in the biosynthesis of phorbol esters (PE) in the Euphorbiaceae. PE are abundant in the seeds of the biofuel crop Jatropha curcas and its toxicity...

    Authors: Natália Pinto de Almeida, Domingos Ferreira Mélo Neto, Gabriel Reis Alves Carneiro, Andreza Raquel Barbosa de Farias, Gilberto Barbosa Domont, Francisco de Assis de Paiva Campos and Fábio César Sousa Nogueira

    Citation: Plant Methods 2021 17:15

    Content type: Research

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  11. The presence of condensed tannins (CT) in tree fodders entails a series of productive, health and ecological benefits for ruminant nutrition. Current wet analytical methods employed for full CT characterisatio...

    Authors: Jordi Ortuño, Sokratis Stergiadis, Anastasios Koidis, Jo Smith, Chris Humphrey, Lindsay Whistance and Katerina Theodoridou

    Citation: Plant Methods 2021 17:14

    Content type: Research

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  12. Paclitaxel is a well-known chemotherapeutic agent widely applied as a therapy for various types of cancers. In vitro culture of Corylus avellana has been named as a promising and low-cost strategy for paclitaxel ...

    Authors: Mina Salehi, Siamak Farhadi, Ahmad Moieni, Naser Safaie and Mohsen Hesami

    Citation: Plant Methods 2021 17:13

    Content type: Research

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  13. Pyropia is an economically advantageous genus of red macroalgae, which has been cultivated in the coastal areas of East Asia for over 300 years. Realizing estimation of macroalgae biomass in a high-throughput way...

    Authors: Shuai Che, Guoying Du, Ning Wang, Kun He, Zhaolan Mo, Bin Sun, Yu Chen, Yifei Cao, Junhao Wang and Yunxiang Mao

    Citation: Plant Methods 2021 17:12

    Content type: Methodology

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  14. Hypericum is an important genus in the family Hypericaceae, which includes 484 species. This genus has been grown in temperate regions and used for treating wounds, eczema and burns. The aim of this study was to ...

    Authors: Maryam Saffariha, Ali Jahani, Reza Jahani and Sajid Latif

    Citation: Plant Methods 2021 17:10

    Content type: Research

    Published on:

  15. The use of non-destructive methods with less human interference is of great interest in agricultural industry and crop breeding. Modern imaging technologies enable the automatic visualization of multi-paramete...

    Authors: Vitor de Jesus Martins Bianchini, Gabriel Moura Mascarin, Lúcia Cristina Aparecida Santos Silva, Valter Arthur, Jens Michael Carstensen, Birte Boelt and Clíssia Barboza da Silva

    Citation: Plant Methods 2021 17:9

    Content type: Methodology

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  16. The absorption, translocation, accumulation and excretion of substances are fundamental processes in all organisms including plants, and have been successfully studied using radiotracers labelled with 11C, 13N, 1...

    Authors: Gihan P. Ruwanpathirana, Darren C. Plett, Robert C. Williams, Catherine E. Davey, Leigh A. Johnston and Herbert J. Kronzucker

    Citation: Plant Methods 2021 17:8

    Content type: Methodology

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  17. Split-root systems (SRS) have many applications in plant sciences, but their implementation, depending on the experimental design, can be difficult and time-consuming. Additionally, the system is not exempt fr...

    Authors: Iñigo Saiz-Fernández, Martin Černý, Jan Skalák and Břetislav Brzobohatý

    Citation: Plant Methods 2021 17:7

    Content type: Research

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  18. Virus-induced gene silencing (VIGS) is a useful tool for functional characterizations of plant genes. However, the penetrance of VIGS varies depending on the genes to be silenced, and has to be evaluated by ex...

    Authors: Kunxin Wu, Yadan Wu, Chunwei Zhang, Yan Fu, Zhixin Liu and Xiuchun Zhang

    Citation: Plant Methods 2021 17:6

    Content type: Research

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  19. Wind strongly impacts plant growth, leaf traits, biomass allocation, and stem mechanical properties. However, whether there are common whole-plant wind responses among different plant species is still unclear....

    Authors: Shudong Zhang, Guofang Liu, Qingguo Cui, Zhenying Huang, Xuehua Ye and Johannes H. C. Cornelissen

    Citation: Plant Methods 2021 17:5

    Content type: Research

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  20. Photosynthetic pigments participating in the absorption, transformation and transfer of light energy play a very important role in plant growth. While, the spatial distribution of foliar pigments is an importa...

    Authors: Jianjun Zeng, Wen Ping, Alireza Sanaeifar, Xiao Xu, Wei Luo, Junjing Sha, Zhenxiong Huang, Yifeng Huang, Xuemei Liu, Baishao Zhan, Hailiang Zhang and Xiaoli Li

    Citation: Plant Methods 2021 17:4

    Content type: Research

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  21. Enzyme assays have widespread applications in drug discovery from plants to natural products. The appropriate use of blanks in enzyme assays is important for assay baseline-correction, and the correction of fa...

    Authors: Chintha Lankatillake, Shiqi Luo, Matthew Flavel, George Binh Lenon, Harsharn Gill, Tien Huynh and Daniel Anthony Dias

    Citation: Plant Methods 2021 17:3

    Content type: Methodology

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  22. Maize haploid breeding technology can be used to rapidly develop homozygous lines, significantly shorten the breeding cycle and improve breeding efficiency. Rapid and accurate sorting haploid kernels is a prer...

    Authors: Yanzhi Qu, Zonghua Liu, Yazhou Zhang, Jiwei Yang and Haochuan Li

    Citation: Plant Methods 2021 17:2

    Content type: Methodology

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  23. Forest dieback driven by rapid climate warming threatens ecosystems worldwide. The health of forested ecosystems depends on how tree species respond to warming during all life history stages. While it is known...

    Authors: E. R. V. Moler, G. Page, L. Flores-Rentería, C. G. Garms, J. B. Hull, H. F. Cooper, J. Swenson, S. Perks, K. M. Waring and A. V. Whipple

    Citation: Plant Methods 2021 17:1

    Content type: Research

    Published on:

  24. Assessment of seed germination is an essential task for seed researchers to measure the quality and performance of seeds. Usually, seed assessments are done manually, which is a cumbersome, time consuming and ...

    Authors: Nikita Genze, Richa Bharti, Michael Grieb, Sebastian J. Schultheiss and Dominik G. Grimm

    Citation: Plant Methods 2020 16:157

    Content type: Methodology

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  25. Flowering is one of the most important processes for flowering plants such as cotton, reflecting the transition from vegetative to reproductive growth and is of central importance to crop yield and adaptabilit...

    Authors: Yu Jiang, Changying Li, Rui Xu, Shangpeng Sun, Jon S. Robertson and Andrew H. Paterson

    Citation: Plant Methods 2020 16:156

    Content type: Methodology

    Published on:

  26. The plant lipidome is highly complex, and the composition of lipids in different tissues as well as their specific functions in plant development, growth and stress responses have yet to be fully elucidated. T...

    Authors: Cheka Kehelpannala, Thusitha W. T. Rupasinghe, Thomas Hennessy, David Bradley, Berit Ebert and Ute Roessner

    Citation: Plant Methods 2020 16:155

    Content type: Methodology

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  27. Pentatricopeptide-repeat proteins (PPRs) characterized by tandem arrays of a degenerate 35-amino-acid repeat (PPR motif) can bind a single strand RNA and regulate organelle gene expression at the post-transcri...

    Authors: Jingli Chen, Haojie Zhu, Jirong Huang and Weihua Huang

    Citation: Plant Methods 2020 16:154

    Content type: Research

    Published on:

  28. Hormones are crucial to plant life and development. Being able to follow the plants hormonal response to various stimuli and throughout developmental processes is an important and increasingly widespread tool....

    Authors: Evyatar Steiner, Alon Israeli, Rupali Gupta, Ido Shwartz, Ido Nir, Meirav Leibman-Markus, Lior Tal, Mika Farber, Ziva Amsalem, Naomi Ori, Bruno Müller and Maya Bar

    Citation: Plant Methods 2020 16:152

    Content type: Methodology

    Published on:

  29. An efficient in vivo transient transfection system using protoplasts is an important tool to study gene expression, metabolic pathways, and multiple mutagenesis parameters in plants. Although rice protoplasts ...

    Authors: Snigdha Poddar, Jaclyn Tanaka, Jamie H. D. Cate, Brian Staskawicz and Myeong-Je Cho

    Citation: Plant Methods 2020 16:151

    Content type: Methodology

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  30. The accurate estimation of potato yield at regional scales is crucial for food security, precision agriculture, and agricultural sustainable development.

    Authors: Shanjun Luo, Yingbin He, Qian Li, Weihua Jiao, Yaqiu Zhu and Xihai Zhao

    Citation: Plant Methods 2020 16:150

    Content type: Research

    Published on:

  31. Mitochondria play critical roles in plant growth, development and stress tolerance. Numerous researchers have carried out studies on the plant mitochondrial genome structure, mitochondrial metabolism and nucle...

    Authors: Yanghong Xu, Xiaoyi Li, Jishuai Huang, Leilei Peng, Dinghui Luo, Qiannan Zhang, Zhiwu Dan, Haijun Xiao, Fang Yang and Jun Hu

    Citation: Plant Methods 2020 16:149

    Content type: Research

    Published on:

  32. The accurate evaluation of the stalk-lodging resistance during the late stage of maize growth can provide a basis for the selection of cultivars, the evaluation of cultivation techniques, and timely mechanical...

    Authors: Jun Xue, Bo Ming, Ruizhi Xie, Keru Wang, Peng Hou and Shaokun Li

    Citation: Plant Methods 2020 16:148

    Content type: Research

    Published on:

  33. High-throughput phenotyping and genomic selection accelerate genetic gain in breeding programs by advances in phenotyping and genotyping methods. This study developed a simple, cost-effective high-throughput i...

    Authors: Morteza Shabannejad, Mohammad-Reza Bihamta, Eslam Majidi-Hervan, Hadi Alipour and Asa Ebrahimi

    Citation: Plant Methods 2020 16:146

    Content type: Research

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  34. Shade avoidance syndrome (SAS) commonly occurs in plants experiencing vegetative shade, causing morphological and physiological changes that are detrimental to plant health and consequently crop yield. As the ...

    Authors: Benny Jian Rong Sng, Gajendra Pratap Singh, Kien Van Vu, Nam-Hai Chua, Rajeev J. Ram and In-Cheol Jang

    Citation: Plant Methods 2020 16:144

    Content type: Research

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  35. Grapevine trunk diseases (GTDs) such as Esca are among the most devastating threats to viticulture. Due to the lack of efficient preventive and curative treatments, Esca causes severe economic losses worldwide...

    Authors: Nele Bendel, Anna Kicherer, Andreas Backhaus, Hans-Christian Klück, Udo Seiffert, Michael Fischer, Ralf T. Voegele and Reinhard Töpfer

    Citation: Plant Methods 2020 16:142

    Content type: Research

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  36. Passion fruit (Passiflora edulis Sims) is an important horticultural crop in the tropics and subtropics, where it has great commercial potential due to high demand for fresh edible fruits and processed juice as w...

    Authors: Lydia K. Asande, Richard O. Omwoyo, Richard O. Oduor and Evans N. Nyaboga

    Citation: Plant Methods 2020 16:141

    Content type: Methodology

    Published on:

  37. Restricting transpiration under high vapor pressure deficit (VPD) is a promising water-saving trait for drought adaptation. However, it is often measured under controlled conditions and at very low throughput,...

    Authors: Soumyashree Kar, Ryokei Tanaka, Lijalem Balcha Korbu, Jana Kholová, Hiroyoshi Iwata, Surya S. Durbha, J. Adinarayana and Vincent Vadez

    Citation: Plant Methods 2020 16:140

    Content type: Research

    Published on:

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