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  1. Variety genuineness and purity are essential indices of maize seed quality that affect yield. However, detection methods for variety genuineness are time-consuming, expensive, require extensive training, or de...

    Authors: Keling Tu, Shaozhe Wen, Ying Cheng, Yanan Xu, Tong Pan, Haonan Hou, Riliang Gu, Jianhua Wang, Fengge Wang and Qun Sun
    Citation: Plant Methods 2022 18:81
  2. The superposition of COVID-19 and climate change has brought great challenges to global food security. As a major economic crop in the world, studying its phenotype to cultivate high-quality wheat varieties is...

    Authors: Anhua Ren, Dong Jiang, Min Kang, Jie Wu, Fangcheng Xiao, Pei Hou and Xiuqing Fu
    Citation: Plant Methods 2022 18:77
  3. In India, raw peanuts are obtained by aggregators from smallholder farms in the form of whole pods and the price is based on a manual estimation of basic peanut pod and kernel characteristics. These methods of...

    Authors: Martha Domhoefer, Debarati Chakraborty, Eva Hufnagel, Joelle Claußen, Norbert Wörlein, Marijn Voorhaar, Krithika Anbazhagan, Sunita Choudhary, Janila Pasupuleti, Rekha Baddam, Jana Kholova and Stefan Gerth
    Citation: Plant Methods 2022 18:76
  4. Optical sensing solutions are being developed and adopted to classify a wide range of biological objects, including crop seeds. Performance assessment of optical classification models remains both a priority a...

    Authors: Christian Nansen, Mohammad S. Imtiaz, Mohsen B. Mesgaran and Hyoseok Lee
    Citation: Plant Methods 2022 18:74
  5. Triticum aestivum is the most important staple food grain of the world. In recent years, the outbreak of a major seed-borne disease, common bunt, in wheat resulted in reduced quality and quantity of the crop. The...

    Authors: Raghav Kataria and Rakesh Kaundal
    Citation: Plant Methods 2022 18:73
  6. Although the genome for the allotetraploid bioenergy crop switchgrass (Panicum virgatum) has been established, limitations in mutant resources have hampered in planta gene function studies toward crop optimizatio...

    Authors: Kira Tiedge, Janessa Destremps, Janet Solano-Sanchez, Magda Lisette Arce-Rodriguez and Philipp Zerbe
    Citation: Plant Methods 2022 18:71
  7. Seeds were an important medium for long-distance transmission of plant viruses. Therefore, appropriate, more sensitive methods for detecting low concentrations of virus-infected in seeds were crucial to ensure...

    Authors: Yin Xinying, Li Xin, Yang Lili, Zheng Qiuyue, Piao Yongzhe and Cao Jijuan
    Citation: Plant Methods 2022 18:70
  8. The bimolecular fluorescence complementation (BiFC) assay has emerged as one of the most popular methods for analysing protein–protein interactions (PPIs) in plant biology. This includes its increasing use as ...

    Authors: Florent Velay, Mélanie Soula, Marwa Mehrez, Clément Belbachir, Stefano D’Alessandro, Christophe Laloi, Patrice Crete and Ben Field
    Citation: Plant Methods 2022 18:69
  9. The chlorophyll content is a vital indicator for reflecting the photosynthesis ability of plants and it plays a significant role in monitoring the general health of plants. Since the chlorophyll content and th...

    Authors: YiFei Cao, Huanliang Xu, Jin Song, Yao Yang, Xiaohui Hu, Korohou Tchalla Wiyao and Zhaoyu Zhai
    Citation: Plant Methods 2022 18:67
  10. The number of banana plants is closely related to banana yield. The diameter and height of the pseudo-stem are important morphological parameters of banana plants, which can reflect the growth status and vital...

    Authors: Yanlong Miao, Liuyang Wang, Cheng Peng, Han Li, Xiuhua Li and Man Zhang
    Citation: Plant Methods 2022 18:66
  11. Quinoa is an increasingly popular seed crop frequently studied for its tolerance to various abiotic stresses as well as its susceptibility to heat. Estimations of quinoa pollen viability through staining metho...

    Authors: S. Elizabeth Castillo, Jose C. Tovar, Anastasia Shamin, Jorge Gutirerrez, Paige Pearson and Malia A. Gehan
    Citation: Plant Methods 2022 18:65
  12. Although quantitative single-cell analysis is frequently applied in animal systems, e.g. to identify novel drugs, similar applications on plant single cells are largely missing. We have exploited the applicabi...

    Authors: Jonathan Dawson, Saurabh Pandey, Qiuju Yu, Patrick Schaub, Florian Wüst, Amir Bahram Moradi, Oleksandr Dovzhenko, Klaus Palme and Ralf Welsch
    Citation: Plant Methods 2022 18:64
  13. The accurate estimation of leaf hydraulic conductance (Kleaf) is important for revealing leaf physiological characteristics and function. However, the Kleaf values are largely incomparable in previous studies for...

    Authors: Xiaoxiao Wang, Jinfang Zhao, Jianliang Huang, Shaobing Peng and Dongliang Xiong
    Citation: Plant Methods 2022 18:63
  14. Rapid-cycling Brassica napus (B. napus-RC) has potential as a rapid trait testing system for canola (B. napus) because its life cycle is completed within 2 months while canola usually takes 4 months, and it is su...

    Authors: Aruni Y. Alahakoon, Eden Tongson, Wei Meng, Zi-Wei Ye, Derek A. Russell, Mee-Len Chye, John F. Golz and Paul W. J. Taylor
    Citation: Plant Methods 2022 18:62
  15. Leaf chlorophyll content plays an important role in indicating plant stresses and nutrient status. Traditional approaches for the quantification of chlorophyll content mainly include acetone ethanol extraction...

    Authors: Huichun Zhang, Yufeng Ge, Xinyan Xie, Abbas Atefi, Nuwan K. Wijewardane and Suresh Thapa
    Citation: Plant Methods 2022 18:60
  16. There is a demand for non-destructive systems in plant phenotyping which could precisely measure plant traits for growth monitoring. In this study, the growth of chilli plants (Capsicum annum L.) was monitored in...

    Authors: Abhipray Paturkar, Gourab Sen Gupta and Donald Bailey
    Citation: Plant Methods 2022 18:59
  17. The study of the regulatory mechanisms of evolutionarily conserved Nucleotide-binding leucine-rich repeat (NLR) resistance (R) proteins in animals and plants is of increasing importance due to understanding ba...

    Authors: Sung Un Huh
    Citation: Plant Methods 2022 18:57

    The Correction to this article has been published in Plant Methods 2022 18:93

  18. Stalk lodging (breaking of agricultural plant stalks prior to harvest) is a multi-billion dollar a year problem. Stalk lodging occurs when high winds induce bending moments in the stalk which exceed the bendin...

    Authors: Christopher J. Stubbs, Christopher S. McMahan, Kaitlin Tabaracci, Bharath Kunduru, Rajandeep S. Sekhon and Daniel J. Robertson
    Citation: Plant Methods 2022 18:56
  19. From an economic perspective, cotton is one of the most important crops in the world. The fertility of male reproductive organs is a key determinant of cotton yield. Anther dehiscence or indehiscence directly ...

    Authors: Zhihao Tan, Jiawei Shi, Rongjie Lv, Qingyuan Li, Jing Yang, Yizan Ma, Yanlong Li, Yuanlong Wu, Rui Zhang, Huanhuan Ma, Yawei Li, Li Zhu, Longfu Zhu, Xianlong Zhang, Jie Kong, Wanneng Yang…
    Citation: Plant Methods 2022 18:53
  20. Anthracnose of Camellia oleifera is a very destructive disease that commonly occurs in the Camellia oleifera industry, which severely restricts the development of the Camellia oleifera industry. In the early stag...

    Authors: Li Bin, Wang Qiu, Zhan Chao-hui, Han Zhao-yang, Yin Hai, Liao Jun and Liu Yan-de
    Citation: Plant Methods 2022 18:52
  21. Illegal logging is a global crisis with significant environmental, economic, and social consequences. Efforts to combat it call for forensic methods to determine species identity, provenance, and individual id...

    Authors: Adriana Costa, Giovanny Giraldo, Amy Bishell, Tuo He, Grant Kirker and Alex C. Wiedenhoeft
    Citation: Plant Methods 2022 18:51
  22. The dry root and rhizome of Salvia miltiorrhiza are used to treat cardiovascular diseases, chronic pain, and thoracic obstruction over 2000 years in Asian countries. For high quality, Sichuan Zhongjiang is regard...

    Authors: Si Cheng Yao, Yuan Yuan Jiang, Su Ni, Long Wang, Jun Feng, Rui Wu Yang, Li Xia Yang, Qiu Yan Len and Li Zhang
    Citation: Plant Methods 2022 18:50
  23. Rice bacterial blight (BB) has caused serious damage in rice yield and quality leading to huge economic loss and food safety problems. Breeding disease resistant cultivar becomes the eco-friendliest and most e...

    Authors: Jinnuo Zhang, Xuping Feng, Qingguan Wu, Guofeng Yang, Mingzhu Tao, Yong Yang and Yong He
    Citation: Plant Methods 2022 18:49
  24. Optimizing plant tissue culture media is a complicated process, which is easily influenced by genotype, mineral nutrients, plant growth regulators (PGRs), vitamins and other factors, leading to undesirable and...

    Authors: Mohammad Sadat-Hosseini, Mohammad M. Arab, Mohammad Soltani, Maliheh Eftekhari, Amanollah Soleimani and Kourosh Vahdati
    Citation: Plant Methods 2022 18:48
  25. Studying dynamic processes in living organisms with MRI is one of the most promising research areas. The use of paramagnetic compounds as contrast agents (CA), has proven key to such studies, but so far, the l...

    Authors: Simon Mayer, Eberhard Munz, Sebastian Hammer, Steffen Wagner, Andre Guendel, Hardy Rolletschek, Peter M. Jakob, Ljudmilla Borisjuk and Thomas Neuberger
    Citation: Plant Methods 2022 18:47
  26. Plant growth devices, for example, rhizoponics, rhizoboxes, and ecosystem fabrication (EcoFAB), have been developed to facilitate studies of plant root morphology and plant-microbe interactions in controlled l...

    Authors: Pipob Suwanchaikasem, Alexander Idnurm, Jamie Selby-Pham, Robert Walker and Berin A. Boughton
    Citation: Plant Methods 2022 18:46
  27. Wild rocket (Diplotaxis tenuifolia) is prone to soil-borne stresses under intensive cultivation systems devoted to ready-to-eat salad chain, increasing needs for external inputs. Early detection of the abiotic an...

    Authors: Alejandra Navarro, Nicola Nicastro, Corrado Costa, Alfonso Pentangelo, Mariateresa Cardarelli, Luciano Ortenzi, Federico Pallottino, Teodoro Cardi and Catello Pane
    Citation: Plant Methods 2022 18:45
  28. Sweet tea, which functions as tea, sugar and medicine, was listed as a new food resource in 2017. Flavonoids are the main medicinal components in sweet tea and have significant pharmacological activities. Ther...

    Authors: Zhaoxia Tian, Zifeng Tan, Yanjie Li and Zhiling Yang
    Citation: Plant Methods 2022 18:44
  29. Perennial fruit trees display a growth behaviour characterized by annual cycling between growth and dormancy, with complex physiological features. Rosaceae fruit trees represent excellent models for studying n...

    Authors: Monica Canton, Silvia Farinati, Cristian Forestan, Justin Joseph, Claudio Bonghi and Serena Varotto
    Citation: Plant Methods 2022 18:43
  30. The construction and application of synthetic genetic circuits is frequently improved if gene expression can be orthogonally controlled, relative to the host. In plants, orthogonality can be achieved via the u...

    Authors: Shaunak Kar, Yogendra Bordiya, Nestor Rodriguez, Junghyun Kim, Elizabeth C. Gardner, Jimmy D. Gollihar, Sibum Sung and Andrew D. Ellington
    Citation: Plant Methods 2022 18:42
  31. Elucidating the genetic and molecular control of plant reproduction often requires the deployment of functional approaches based on reverse or forward genetic screens. The loss-of-function of essential genes, ...

    Authors: Jasmin Schubert, Yanru Li, Marta A. Mendes, Danli Fei, Hugh Dickinson, Ian Moore and Célia Baroux
    Citation: Plant Methods 2022 18:41
  32. Stable isotope labeling is a non-invasive, sensitive means of monitoring metabolic flux in plants. The most physiologically meaningful information is obtained from experiments that take advantage of the natura...

    Authors: Sonia E. Evans, Peter Duggan, Matthew E. Bergman, Daniela Cobo-López, Benjamin Davis, Ibadat Bajwa and Michael A. Phillips
    Citation: Plant Methods 2022 18:40
  33. Roots are vital to plant performance because they acquire resources from the soil and provide anchorage. However, it remains difficult to assess root system size and distribution because roots are inaccessible...

    Authors: Ashish B. Rajurkar, Scott M. McCoy, Jeremy Ruhter, Jessica Mulcrone, Luke Freyfogle and Andrew D. B. Leakey
    Citation: Plant Methods 2022 18:39
  34. Profiling the plant root architecture is vital for selecting resilient crops that can efficiently take up water and nutrients. The high-performance imaging tools available to study root-growth dynamics with th...

    Authors: Vinicius Lube, Mehmet Alican Noyan, Alexander Przybysz, Khaled Salama and Ikram Blilou
    Citation: Plant Methods 2022 18:38
  35. Plant DNA isolation and purification is a time-consuming and laborious process relative to epithelial and viral DNA sample preparation due to the cell wall. The lysis of plant cells to free intracellular DNA n...

    Authors: Miranda N. Emaus, Cecilia Cagliero, Morgan R. Gostel, Gabriel Johnson and Jared L. Anderson
    Citation: Plant Methods 2022 18:37
  36. Soybean iron deficiency chlorosis (IDC) is an important nutrient stress frequently found in high pH and/or soils high in calcium carbonates. To advance the understanding of IDC resistance in soybean, a rapid (...

    Authors: R. Merry, M. J. Espina, A. J. Lorenz and R. M. Stupar
    Citation: Plant Methods 2022 18:36
  37. Plant pathogens cause yield losses in crops worldwide. Breeding for improved disease resistance and management by precision agriculture are two approaches to limit such yield losses. Both rely on detecting and...

    Authors: Florian Tanner, Sebastian Tonn, Jos de Wit, Guido Van den Ackerveken, Bettina Berger and Darren Plett
    Citation: Plant Methods 2022 18:35
  38. In addition to heterogeneity and artificial selection, natural selection is one of the forces used to combat climate change and improve agrobiodiversity in evolutionary plant breeding. Accurate identification ...

    Authors: Michael Schneider, Asis Shrestha, Agim Ballvora and Jens Léon
    Citation: Plant Methods 2022 18:34
  39. The genetic engineering of crops has enhanced productivity in the face of climate change and a growing global population by conferring desirable genetic traits, including the enhancement of biotic and abiotic ...

    Authors: Lamiaa M. Mahmoud, Prabhjot Kaur, Daniel Stanton, Jude W. Grosser and Manjul Dutt
    Citation: Plant Methods 2022 18:33
  40. Traditional CRISPR/Cas9 systems that rely on U6 or U3 snRNA promoters (RNA polymerase III-dependent promoters) can only achieve constitutive gene editing in plants, hampering the functional analysis of specifical...

    Authors: Jia-Wen Li, Tuo Zeng, Zhi-Zhuo Xu, Jin-Jin Li, Hao Hu, Qin Yu, Li Zhou, Ri-Ru Zheng, Jing Luo and Cai-Yun Wang
    Citation: Plant Methods 2022 18:32
  41. In order to improve the current situations in rice–wheat rotation region in the middle and lower reaches of the Yangtze River, such as large amount of rice straw, complex returning process, short-time stubble ...

    Authors: Yinyan Shi, Ye Jiang, Xiaochan Wang, Haiming Yu, Hui Liu and Jingbo Chen
    Citation: Plant Methods 2022 18:31
  42. High-throughput plant phenotyping (HTPP) methods have the potential to speed up the crop breeding process through the development of cost-effective, rapid and scalable phenotyping methods amenable to automatio...

    Authors: Alexander Koc, Firuz Odilbekov, Marwan Alamrani, Tina Henriksson and Aakash Chawade
    Citation: Plant Methods 2022 18:30

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