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  1. The aim of this study was to evaluate and characterize the mutations induced by two TALE-based approaches, double-strand break (DSB) induction by the FokI nuclease (mitoTALEN) and targeted base editing by the Ddd...

    Authors: Alessandro Nicolia, Nunzia Scotti, Nunzio D’Agostino, Giovanna Festa, Lorenza Sannino, Gaetano Aufiero, Shin-ichi Arimura and Teodoro Cardi
    Citation: Plant Methods 2024 20:4
  2. Populus simonii × P. nigra is an ideal material for studying the molecular mechanisms of woody plants. In recent years, research on Populus simonii × P. nigra has increasingly focused on the application of transg...

    Authors: Ping Yang, Yao Sun, Xin Sun, Yao Li and Lei Wang
    Citation: Plant Methods 2024 20:3
  3. The chloroplast genome (cp genome) is directly related to the study and analysis of molecular phylogeny and evolution of plants in the phylogenomics era. The cp genome, whereas, is highly plastic and exists as...

    Authors: Qiang Zhou, Xianlong Ding, Hongjie Wang, Zunaira Farooq, Liang Wang and Shouping Yang
    Citation: Plant Methods 2024 20:2
  4. Tea is the most popular beverage worldwide second only to water. Its demand is tremendously rising due to increased awareness of its medicinal importance. The quality and uses of tea depend on the tea-types wh...

    Authors: Abhishek Kumar, Nikhil Rawat, Shweta Thakur, Rohit Joshi and Shiv Shanker Pandey
    Citation: Plant Methods 2023 19:147
  5. Grapevine berries undergo asynchronous growth and ripening dynamics within the same bunch. Due to the lack of efficient methods to perform sequential non-destructive measurements on a representative number of ...

    Authors: Benoit Daviet, Christian Fournier, Llorenç Cabrera-Bosquet, Thierry Simonneau, Maxence Cafier and Charles Romieu
    Citation: Plant Methods 2023 19:146
  6. Crop pests reduce productivity, so managing them through early detection and prevention is essential. Data from various modalities are being used to predict crop diseases by applying machine learning methodolo...

    Authors: Sangyeon Lee and Choa Mun Yun
    Citation: Plant Methods 2023 19:145

    The Correction to this article has been published in Plant Methods 2024 20:24

  7. Understanding thiamin (thiamine; vitamin B1) metabolism in plants is crucial, as it impacts plant nutritional value as well as stress tolerance. Studies aimed at elucidating novel aspects of thiamin in plants rel...

    Authors: Simon Strobbe, Jana Verstraete, Teresa B. Fitzpatrick, Christophe Stove and Dominique Van Der Straeten
    Citation: Plant Methods 2023 19:144
  8. The Amaranthus genus contains at least 20 weedy and invasive species, including Amaranthus palmeri (palmer’s amaranth) and Amaranthus tuberculatus (tall waterhemp), two species of regulatory concern in North Amer...

    Authors: Maxime Murphy, Julia Hubert, Ruojing Wang and Leonardo Galindo-González
    Citation: Plant Methods 2023 19:143
  9. Plant defense activators offer advantages over pesticides by avoiding the emergence of drug-resistant pathogens. However, only a limited number of compounds have been reported. Reactive oxygen species (ROS) ac...

    Authors: Masayuki Kogoshi, Daiki Nishio, Nobutaka Kitahata, Hayato Ohwada, Kazuyuki Kuchitsu, Hideyuki Mizuno and Takamitsu Kurusu
    Citation: Plant Methods 2023 19:142
  10. Protein–protein interactions (PPIs) are heavily involved in many biological processes. Consequently, the identification of PPIs in the model plant Arabidopsis is of great significance to deeply understand plan...

    Authors: Kewei Zhou, Chenping Lei, Jingyan Zheng, Yan Huang and Ziding Zhang
    Citation: Plant Methods 2023 19:141
  11. For molecular breeding of future apples, wild apple (Malus sieversii), the primary progenitor of domesticated apples, provides abundant genetic diversity and disease-resistance traits. Valsa canker (caused by the...

    Authors: Xuejing Wen, Jiangxue Yuan, Tohir A. Bozorov, Abdul Waheed, Gulnaz Kahar, Yakupjan Haxim, Xiaojie Liu, Lili Huang and Daoyuan Zhang
    Citation: Plant Methods 2023 19:138
  12. Doubled haploid technology, which enables the generation of homozygous lines in a single step, is one of the modern tools being employed for accelerating breeding processes in different crops. In rice, a globa...

    Authors: Chaitanya Ghalagi, Malavalli Rajashekar Namratha, Kavita Kotyal, Shiva Prakash and Basavaiah Mohan Raju
    Citation: Plant Methods 2023 19:137
  13. Lily is a bulbous plant with an endogenous dormancy trait. Fine-tuning bulb dormancy release is still a challenge in the development of bulb storage technology. In this study, we identified three regulators of...

    Authors: Yajie Zhao, Wenqiang Pan, Yin Xin, Jingxiang Wu, Rong Li, Jinxin Shi, Shuo Long, Lianwei Qu, Yingdong Yang, Mingfang Yi and Jian Wu
    Citation: Plant Methods 2023 19:136
  14. Calcium oxalate (CaOx) is the most prevalent and widespread biomineral in plants and is involved in protective and/or defensive functions against abiotic stress factors. It is, however, expected that this func...

    Authors: Changying Li, Chunli Chen, Lihong Qin, Dengyue Zheng, Qian Du, Qiandong Hou and Xiaopeng Wen
    Citation: Plant Methods 2023 19:135
  15. The metrics for assessing the yield of crops in the field include the number of ears per unit area, the grain number per ear, and the thousand-grain weight. Typically, the ear number per unit area contributes ...

    Authors: Yangyang Gu, Hongxu Ai, Tai Guo, Peng Liu, Yongqing Wang, Hengbiao Zheng, Tao Cheng, Yan Zhu, Weixing Cao and Xia Yao
    Citation: Plant Methods 2023 19:134
  16. Thermography is a popular tool to assess plant water-use behavior, as plant temperature is influenced by transpiration rate, and is commonly used in field experiments to detect plant water deficit. Its applica...

    Authors: Stien Mertens, Lennart Verbraeken, Heike Sprenger, Sam De Meyer, Kirin Demuynck, Bernard Cannoot, Julie Merchie, Jolien De Block, Jonathan T. Vogel, Wesley Bruce, Hilde Nelissen, Steven Maere, Dirk Inzé and Nathalie Wuyts
    Citation: Plant Methods 2023 19:132
  17. In the past few years, there has been an explosion in single-cell transcriptomics datasets, yet in vivo confirmation of these datasets is hampered in plants due to lack of robust validation methods. Likewise, ...

    Authors: Tian Huang, Bruno Guillotin, Ramin Rahni, Kenneth D. Birnbaum and Doris Wagner
    Citation: Plant Methods 2023 19:131
  18. The evolution of plants to efficiently transport water and assimilates over long distances is a major evolutionary success that facilitated their growth and colonization of land. Vascular tissues, namely xylem...

    Authors: Karolina Kułak, Natalia Wojciechowska, Anna Samelak-Czajka, Paulina Jackowiak and Agnieszka Bagniewska-Zadworna
    Citation: Plant Methods 2023 19:129
  19. Survival rate (SR) is frequently used to compare drought tolerance among plant genotypes. While a variety of techniques for evaluating the stress status of plants under drought stress conditions have been deve...

    Authors: Thelma Y. Rico-Cambron, Elohim Bello-Bello, Octavio Martínez and Luis Herrera-Estrella
    Citation: Plant Methods 2023 19:127
  20. Object detection, size determination, and colour detection of images are tools commonly used in plant science. Key examples of this include identification of ripening stages of fruit such as tomatoes and the d...

    Authors: Harry Charles Wright, Frederick Antonio Lawrence, Anthony John Ryan and Duncan Drummond Cameron
    Citation: Plant Methods 2023 19:126
  21. Inflorescence properties such length, spikelet number, and their spatial distribution across the rachis, are fundamental indicators of seed productivity in grasses and have been a target of selection throughou...

    Authors: Joan Barreto Ortiz, Candice N. Hirsch, Nancy Jo Ehlke and Eric Watkins
    Citation: Plant Methods 2023 19:125
  22. Manual analysis of (mini-)rhizotron (MR) images is tedious. Several methods have been proposed for semantic root segmentation based on homogeneous, single-source MR datasets. Recent advances in deep learning (...

    Authors: Pavel Baykalov, Bart Bussmann, Richard Nair, Abraham George Smith, Gernot Bodner, Ofer Hadar, Naftali Lazarovitch and Boris Rewald
    Citation: Plant Methods 2023 19:122
  23. The mutualistic interaction between entomophilous plants and pollinators is fundamental to the structure of most terrestrial ecosystems. The sensitive nature of this relationship has been disrupted by anthropo...

    Authors: Sydney B. Wizenberg, Laura R. Newburn, Mateus Pepinelli, Ida M. Conflitti, Rodney T. Richardson, Shelley E. R. Hoover, Robert W. Currie, Pierre Giovenazzo and Amro Zayed
    Citation: Plant Methods 2023 19:120
  24. Single-cell RNA sequencing (scRNA-seq) measurements of gene expression show great promise for studying the cellular heterogeneity of rice roots. How precisely annotating cell identity is a major unresolved pro...

    Authors: Hao Wang, Yu-Nan Lin, Shen Yan, Jing-Peng Hong, Jia-Rui Tan, Yan-Qing Chen, Yong-Sheng Cao and Wei Fang
    Citation: Plant Methods 2023 19:119
  25. Crop breeding should be accelerated to address global warming and climate change. Wheat (Triticum aestivum L.) is a major food crop. Speed breeding (SB) and speed vernalization (SV) techniques for spring and wint...

    Authors: Jin-Kyung Cha, Hyeonjin Park, Changhyun Choi, Youngho Kwon, So-Myeong Lee, Ki-Won Oh, Jong-Min Ko, Soon-Wook Kwon and Jong-Hee Lee
    Citation: Plant Methods 2023 19:118
  26. Although previous studies on the droplet deposition behaviour of rice leaves have modelled the leaves as flat surface structures, their curved surface structures actually have a significant effect on droplet d...

    Authors: He Zheng, Hao Sun, Yubin Cao, Xiaolan Lv, Chaoxi Wang, Yunfu Chen, Hongfeng Yu and Wei Qiu
    Citation: Plant Methods 2023 19:116
  27. Spike is the grain-bearing organ in cereal crops, which is a key proxy indicator determining the grain yield and quality. Machine learning methods for image analysis of spike-related phenotypic traits not only...

    Authors: Yimin Ling, Qinlong Zhao, Wenxin Liu, Kexu Wei, Runfei Bao, Weining Song and Xiaojun Nie
    Citation: Plant Methods 2023 19:115
  28. Rubus species holds promise as a valuable source of polyphenols and bioactive compounds, offering significant potential as functional food ingredients with both nutraceutical and pharmaceutical benefits. However,...

    Authors: Saurav Chandra Bhatt, Bindu Naik, Vijay Kumar, Arun Kumar Gupta, Sanjay Kumar, Manpreet Singh Preet, Nitya Sharma and Sarvesh Rustagi
    Citation: Plant Methods 2023 19:114
  29. Protein farnesylation involves the addition of a 15-carbon polyunsaturated farnesyl group to proteins whose C-terminus ends with a CaaX motif. This post-translational protein modification is catalyzed by a het...

    Authors: Jia-Rong Wu, Rida Zohra, Ngoc Kieu Thi Duong, Ching-Hui Yeh, Chung-An Lu and Shaw-Jye Wu
    Citation: Plant Methods 2023 19:113
  30. Long read sequencing allows the analysis of full-length transcripts in plants without the challenges of reliable transcriptome assembly. Long read sequencing of transcripts from plant genomes has often utilize...

    Authors: Othman Al-Dossary, Agnelo Furtado, Ardashir KharabianMasouleh, Bader Alsubaie, Ibrahim Al-Mssallem and Robert J. Henry
    Citation: Plant Methods 2023 19:112
  31. This study aims to establish cell suspension culture, its maintenance and induction of somatic embryogenesis, and in vitro plant regeneration in Cenchrus ciliaris L. Suspension cultures are relatively homogenous ...

    Authors: Shipra Goyal, Vijaya Chatterjee, Vishvas M. Kulkarni and Vishnu Bhat
    Citation: Plant Methods 2023 19:110
  32. Remote sensing of vegetation by spectroscopy is increasingly used to characterize trait distributions in plant communities. How leaves interact with electromagnetic radiation is determined by their structure a...

    Authors: Cheng Li, Ewa A. Czyż, Rayko Halitschke, Ian T. Baldwin, Michael E. Schaepman and Meredith C. Schuman
    Citation: Plant Methods 2023 19:108
  33. The field of plant hormonomics focuses on the qualitative and quantitative analysis of the hormone complement in plant samples, akin to other omics sciences. Plant hormones, alongside primary and secondary met...

    Authors: Ondřej Vrobel and Petr Tarkowski
    Citation: Plant Methods 2023 19:107
  34. Modern field pea breeding faces a significant challenge in selecting lines with strong stems that resist lodging. Traditional methods of assessing stem strength involve destructive mechanical tests on mature s...

    Authors: Qi Fang, Felipe A. Castro-Urrea, Felix Haederle, Rowan W. Sanderson, Dilusha Silva, Wallace A. Cowling and Brendan F. Kennedy
    Citation: Plant Methods 2023 19:105
  35. Since its discovery, somatic hybridization has been used to overcome the sexual barriers between cultivated and wild species. A combination of two somatic cells might provide a novel set of features, often of ...

    Authors: Katarzyna Mackowska, Katarzyna Stelmach-Wityk and Ewa Grzebelus
    Citation: Plant Methods 2023 19:104
  36. Detection and counting of wheat heads are of crucial importance in the field of plant science, as they can be used for crop field management, yield prediction, and phenotype analysis. With the widespread appli...

    Authors: Jianxiong Ye, Zhenghong Yu, Yangxu Wang, Dunlu Lu and Huabing Zhou
    Citation: Plant Methods 2023 19:103

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