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  1. Content type: Methodology

    Hyperspectral imaging is a technology that can be used to monitor plant responses to stress. Hyperspectral images have a full spectrum for each pixel in the image, 400–2500 nm in this case, giving detailed inf...

    Authors: Dominic Williams, Avril Britten, Susan McCallum, Hamlyn Jones, Matt Aitkenhead, Alison Karley, Ken Loades, Ankush Prashar and Julie Graham

    Citation: Plant Methods 2017 13:74

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  2. Content type: Research

    Flos Chrysanthemi, as a part of Chinese culture for a long history, is valuable for not only environmental decoration but also the medicine and food additive. Due to their voluminously...

    Authors: Heng Zhang, Zhenyi Chen, Taihao Li, Na Chen, Wenjie Xu and Shupeng Liu

    Citation: Plant Methods 2017 13:92

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  3. Content type: Research

    Cultivated strawberry (Fragaria × ananassa Duch.) has homoeologous chromosomes because of allo-octoploidy. For example, two homoeologous chromosomes that belong to different sub-genome of allopolyploids have simi...

    Authors: Tomohiro Yanagi, Kenta Shirasawa, Mayuko Terachi and Sachiko Isobe

    Citation: Plant Methods 2017 13:91

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  4. Content type: Research

    There is currently a high requirement for field phenotyping methodologies/technologies to determine quantitative traits related to crop yield and plant stress responses under field conditions.

    Authors: Jorge Gago, Alisdair R. Fernie, Zoran Nikoloski, Takayuki Tohge, Sebastiá Martorell, José Mariano Escalona, Miquel Ribas-Carbó, Jaume Flexas and Hipólito Medrano

    Citation: Plant Methods 2017 13:90

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  5. Content type: Methodology

    Cytokinins play many essential roles in plant growth and development, mainly through signal transduction pathways. Although the cytokinin signaling pathway in rice has been clarified, no synthetic reporter for...

    Authors: Jinyuan Tao, Huwei Sun, Pengyuan Gu, Zhihao Liang, Xinni Chen, Jiajing Lou, Guohua Xu and Yali Zhang

    Citation: Plant Methods 2017 13:89

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  6. Content type: Methodology

    The oomycete Phytophthora infestans causes late blight on potato and tomato. Despite extensive research, the P. infestans-host interaction is still poorly understood. To find new ways to further unravel this inte...

    Authors: Charikleia Schoina, Klaas Bouwmeester and Francine Govers

    Citation: Plant Methods 2017 13:88

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  7. Content type: Methodology

    Most current methods for constructing guide RNAs (gRNA) for the CRISPR/Cas9 genome editing system, depend on traditional cloning using specific type IIS restriction enzymes and DNA ligation. These methods cons...

    Authors: Aftab A. Khan, Ashraf El-Sayed, Asma Akbar, Arianna Mangravita-Novo, Shaheen Bibi, Zunaira Afzal, David J. Norman and Gul Shad Ali

    Citation: Plant Methods 2017 13:86

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  8. Content type: Methodology

    Genomic DNA extracted from species of Cactaceae is often contaminated with significant amounts of mucilage and pectin. Pectin is one of the main components of cellular walls, whereas mucilage is a complex poly...

    Authors: César Ramiro Martínez-González, Rosario Ramírez-Mendoza, Jaime Jiménez-Ramírez, Clemente Gallegos-Vázquez and Isolda Luna-Vega

    Citation: Plant Methods 2017 13:82

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  9. Content type: Review

    This review explores how imaging techniques are being developed with a focus on deployment for crop monitoring methods. Imaging applications are discussed in relation to both field and glasshouse-based plants,...

    Authors: Amy Lowe, Nicola Harrison and Andrew P French

    Citation: Plant Methods 2017 13:80

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  10. Content type: Research

    Floral timing is a carefully regulated process, in which the plant determines the optimal moment to switch from the vegetative to reproductive phase. While there are numerous genes known that control flowering...

    Authors: Martijn Fiers, Jorin Hoogenboom, Alice Brunazzi, Tom Wennekes, Gerco C. Angenent and Richard G. H. Immink

    Citation: Plant Methods 2017 13:78

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  11. Content type: Methodology article

    The complex cell wall structure of algae often precludes efficient extraction of their genetic material. The purpose of this study was to design a next-generation sequencing-suitable DNA isolation method for u...

    Authors: Tomasz Jagielski, Jan Gawor, Zofia Bakuła, Karolina Zuchniewicz, Iwona Żak and Robert Gromadka

    Citation: Plant Methods 2017 13:77

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  12. Content type: Research

    Changing the phosphorus (P) nutrition leads to changes in plant metabolism. The aim of this study was to investigate how these changes are reflected in the distribution of 33P and the isotopic composition of oxy...

    Authors: Verena Pfahler, Federica Tamburini, Stefano M. Bernasconi and Emmanuel Frossard

    Citation: Plant Methods 2017 13:75

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  13. Content type: Research

    Phenotyping technologies are expected to provide predictive power for a range of applications in plant and crop sciences. Here, we use the disease pressure of Beet Cyst Nematodes (BCN) on sugar beet as an illu...

    Authors: Samuel Joalland, Claudio Screpanti, Frank Liebisch, Hubert Vincent Varella, Alain Gaume and Achim Walter

    Citation: Plant Methods 2017 13:73

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  14. Content type: Methodology article

    Celiac disease (CD) is caused by specific sequences of gluten proteins found in cereals such as bread wheat (Triticum aestivum ssp. aestivum) and spelt (T. aestivum ssp. spelta). Among them, the α-gliadins displa...

    Authors: Benjamin Dubois, Pierre Bertin, Yordan Muhovski, Emmanuelle Escarnot and Dominique Mingeot

    Citation: Plant Methods 2017 13:72

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  15. Content type: Methodology article

    Seed–soil contact is important to ensure successful germination, however, there is a paucity of reported studies that have quantified the microstructure at and around this critical interface, mainly due to the...

    Authors: Sebastian Blunk, Ali Hafeez Malik, Martine I. de Heer, Tobias Ekblad, Jennifer Bussell, Debbie Sparkes, Kenneth Fredlund, Craig J. Sturrock and Sacha J. Mooney

    Citation: Plant Methods 2017 13:71

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  16. Content type: Research

    Boron (B) tolerance has been identified as a key target for field pea improvement. Screening for B tolerance in the field is problematic due to variability in space and time, and robust B molecular markers are...

    Authors: Richard G. Bennett, Federico M. Ribalta, Maria Pazos-Navarro, Antonio Leonforte and Janine S. Croser

    Citation: Plant Methods 2017 13:70

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  17. Content type: Methodology article

    Sedentary endoparasitic cyst nematodes form a feeding structure in plant roots, called a syncytium. Syncytium formation involves extensive transcriptional modifications, which leads to cell modifications such ...

    Authors: Rinu Kooliyottil, Louise-Marie Dandurand, Joseph C. Kuhl, Allan Caplan and Fangming Xiao

    Citation: Plant Methods 2017 13:68

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  18. Content type: Methodology article

    Seed development in the angiosperms requires the production of a female gametophyte (embryo sac) within the ovule. Many aspects of female reproductive development in cereal crops are yet to be described, large...

    Authors: Laura G. Wilkinson and Matthew R. Tucker

    Citation: Plant Methods 2017 13:67

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  19. Content type: Methodology

    Common cutworm (CCW; Spodoptera litura Fabricius) is a major leaf-feeding pest of soybean in Asia. The previous methods of measuring antixenosis against CCW using adult plant under field or net-room conditions we...

    Authors: Guangnan Xing, Kai Liu and Junyi Gai

    Citation: Plant Methods 2017 13:66

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  20. Content type: Research

    Understanding root traits is a necessary research front for selection of favorable genotypes or cultivation practices. Root and tuber crops having most of their economic potential stored below ground are favor...

    Authors: Alfredo Delgado, Dirk B. Hays, Richard K. Bruton, Hernán Ceballos, Alexandre Novo, Enrico Boi and Michael Gomez Selvaraj

    Citation: Plant Methods 2017 13:65

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  21. Content type: Research

    Plant glutathione S-transferases (GSTs, EC are multifunctional enzymes involved in heavy metal cellular detoxification by conjugating the tripeptide (g-Glu-Cys-Gly) glutathione to heavy metals. Previous...

    Authors: Zhenzhen Cao, Renxiang Mou, Zhaoyun Cao, Xiaoyan Lin, Youning Ma, Zhiwei Zhu and Mingxue Chen

    Citation: Plant Methods 2017 13:64

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  22. Content type: Methodology article

    Condensed tannins (CT) are the most abundant secondary metabolite of land plants and can vary in abundance and structure according to tissue type, species, genotype, age, and environmental conditions. Recent i...

    Authors: Philip-Edouard Shay, J. A. Trofymow and C. Peter Constabel

    Citation: Plant Methods 2017 13:63

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  23. Content type: Methodology article

    Modern agriculture uses hyperspectral cameras that provide hundreds of reflectance data at discrete narrow bands in many environments. These bands often cover the whole visible light spectrum and part of the i...

    Authors: Abelardo Montesinos-López, Osval A. Montesinos-López, Jaime Cuevas, Walter A. Mata-López, Juan Burgueño, Sushismita Mondal, Julio Huerta, Ravi Singh, Enrique Autrique, Lorena González-Pérez and José Crossa

    Citation: Plant Methods 2017 13:62

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  24. Content type: Methodology article

    Photosynthesis can be roughly separated into biochemical and photochemical processes. Both are affected by drought and can be assessed by non-invasive standard methods. Gas exchange, which mainly assesses the ...

    Authors: Carlos Antônio Ferreira de Sousa, Dayane Silva de Paiva, Raphael Augusto das Chagas Noqueli Casari, Nelson Geraldo de Oliveira, Hugo Bruno Correa Molinari, Adilson Kenji Kobayashi, Paulo Cesar Magalhães, Reinaldo Lúcio Gomide and Manoel Teixeira Souza Jr.

    Citation: Plant Methods 2017 13:61

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  25. Content type: Methodology article

    Virus-induced gene silencing (VIGS) has been used in many plant species as an attractive post transcriptional gene silencing (PTGS) method for studying gene function either individually or at large-scale in a ...

    Authors: Bangjun Zhou and Lirong Zeng

    Citation: Plant Methods 2017 13:59

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  26. Content type: Methodology

    There are numerous systems and techniques to measure the growth of plant roots. However, phenotyping large numbers of plant roots for breeding and genetic analyses remains challenging. One major difficulty is ...

    Authors: Lionel X. Dupuy, Gladys Wright, Jacqueline A. Thompson, Anna Taylor, Sebastien Dekeyser, Christopher P. White, William T. B. Thomas, Mark Nightingale, John P. Hammond, Neil S. Graham, Catherine L. Thomas, Martin R. Broadley and Philip J. White

    Citation: Plant Methods 2017 13:57

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  27. Content type: Methodology

    In sorghum, the growth angle of nodal roots is a major component of root system architecture. It strongly influences the spatial distribution of roots of mature plants in the soil profile, which can impact dro...

    Authors: Dinesh C. Joshi, Vijaya Singh, Colleen Hunt, Emma Mace, Erik van Oosterom, Richard Sulman, David Jordan and Graeme Hammer

    Citation: Plant Methods 2017 13:56

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  28. Content type: Methodology article

    Tree pruning is a costly practice with important implications for crop harvest and nutrition, pest and disease control, soil protection and irrigation strategies. Investigations on tree pruning usually involve...

    Authors: F. M. Jiménez-Brenes, F. López-Granados, A. I. de Castro, J. Torres-Sánchez, N. Serrano and J. M. Peña

    Citation: Plant Methods 2017 13:55

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  29. Content type: Methodology

    Automated plant phenotyping has been established as a powerful new tool in studying plant growth, development and response to various types of biotic or abiotic stressors. Respective facilities mainly apply no...

    Authors: Henning Tschiersch, Astrid Junker, Rhonda C. Meyer and Thomas Altmann

    Citation: Plant Methods 2017 13:54

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  30. Content type: Research

    Low field NMR has been used to investigate water status in various plant tissues. In plants grown in controlled conditions, the method was shown to be able to monitor leaf development as it could detect slight...

    Authors: Maja Musse, Laurent Leport, Mireille Cambert, William Debrandt, Clément Sorin, Alain Bouchereau and François Mariette

    Citation: Plant Methods 2017 13:53

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  31. Content type: Research

    Plant water resource management is one of the main future challenges to fight recent climatic changes. The knowledge of the plant water content could be indispensable for water saving strategies. Terahertz spe...

    Authors: Lorenzo Baldacci, Mario Pagano, Luca Masini, Alessandra Toncelli, Giorgio Carelli, Paolo Storchi and Alessandro Tredicucci

    Citation: Plant Methods 2017 13:51

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  32. Content type: Research

    Precise control of gene expression is essential to synchronize plant development with the environment. In perennial plants, transcriptional regulation remains poorly understood, mainly due to the long time req...

    Authors: José M. Ramos-Sánchez, Paolo M. Triozzi, Alicia Moreno-Cortés, Daniel Conde, Mariano Perales and Isabel Allona

    Citation: Plant Methods 2017 13:50

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  33. Content type: Methodology

    Within the Brassicaceae, six species from the genus Brassica are widely cultivated throughout the world as oilseed, condiment, fodder or vegetable crops. The genetic relationships among the six Brassica species a...

    Authors: Joshua C. O. Koh, Denise M. Barbulescu, Sally Norton, Bob Redden, Phil A. Salisbury, Sukhjiwan Kaur, Noel Cogan and Anthony T. Slater

    Citation: Plant Methods 2017 13:49

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  34. Content type: Methodology

    In order to better understand and improve the mode of action of agrochemicals, it is useful to be able to visualize their uptake and distribution in vivo, non-invasively and, ideally, in the field. Here we exp...

    Authors: Elizabeth Noble, Sunil Kumar, Frederik G. Görlitz, Chris Stain, Chris Dunsby and Paul M. W. French

    Citation: Plant Methods 2017 13:48

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  35. Content type: Methodology

    Hyperspectral imaging is an emerging means of assessing plant vitality, stress parameters, nutrition status, and diseases. Extraction of target values from the high-dimensional datasets either relies on pixel-...

    Authors: Uwe Knauer, Andrea Matros, Tijana Petrovic, Timothy Zanker, Eileen S. Scott and Udo Seiffert

    Citation: Plant Methods 2017 13:47

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  36. Content type: Methodology

    Perennial ryegrass (Lolium perenne L.) is an important temperate grass used for turf and forage purposes. With the increasing accumulation of genomic and transcriptomic data of perennial ryegrass, an efficient pr...

    Authors: Guohui Yu, Qiang Cheng, Zheni Xie, Bin Xu, Bingru Huang and Bingyu Zhao

    Citation: Plant Methods 2017 13:46

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  37. Content type: Methodology

    Chlorophyll fluorescence (CF) is a key indicator to study plant physiology or photosynthesis efficiency. Conventionally, CF is characterized by fluorometers, which only allows ensemble measurement through wide...

    Authors: Yi-Chin Tseng and Shi-Wei Chu

    Citation: Plant Methods 2017 13:43

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  38. Content type: Methodology

    Binding of transcription factors to their target sequences is a primary step in the regulation of gene expression and largely determines gene regulatory networks. Chromatin immunoprecipitation (ChIP) is an ind...

    Authors: Jeong Hwan Lee, Suhyun Jin, Sun Young Kim, Wanhui Kim and Ji Hoon Ahn

    Citation: Plant Methods 2017 13:42

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  39. Content type: Research

    The main aim of this study was to improve fungal resistance in bread wheat via transgenesis. Transgenic wheat plants harboring barley chitinase (chi26) gene, driven by maize ubi promoter, were obtained using biol...

    Authors: Hala F. Eissa, Sameh E. Hassanien, Ahmed M. Ramadan, Moustafa M. El-Shamy, Osama M. Saleh, Ahmed M. Shokry, Mohamed Abdelsattar, Yasser B. Morsy, Maher A. El-Maghraby, Hussien F. Alameldin, Sabah M. Hassan, Gamal H. Osman, Hesham T. Mahfouz, Gharib A. Gad El-Karim, Magdy A. Madkour and Ahmed Bahieldin

    Citation: Plant Methods 2017 13:41

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