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  1. Methodology

    Growth curve registration for evaluating salinity tolerance in barley

    Smarthouses capable of non-destructive, high-throughput plant phenotyping collect large amounts of data that can be used to understand plant growth and productivity in extreme environments. The challenge is to...

    Rui Meng, Stephanie Saade, Sebastian Kurtek, Bettina Berger, Chris Brien, Klaus Pillen, Mark Tester and Ying Sun

    Plant Methods 2017 13:18

    Published on: 23 March 2017

  2. Methodology

    An image analysis pipeline for automated classification of imaging light conditions and for quantification of wheat canopy cover time series in field phenotyping

    Robust segmentation of canopy cover (CC) from large amounts of images taken under different illumination/light conditions in the field is essential for high throughput field phenotyping (HTFP). We attempted to...

    Kang Yu, Norbert Kirchgessner, Christoph Grieder, Achim Walter and Andreas Hund

    Plant Methods 2017 13:15

    Published on: 21 March 2017

  3. Methodology

    The combination of gas-phase fluorophore technology and automation to enable high-throughput analysis of plant respiration

    Mitochondrial respiration in the dark (R dark) is a critical plant physiological process, and hence a reliable, efficient and high-throughput method of measuring variation in rates of R

    Andrew P. Scafaro, A. Clarissa A. Negrini, Brendan O’Leary, F. Azzahra Ahmad Rashid, Lucy Hayes, Yuzhen Fan, You Zhang, Vincent Chochois, Murray R. Badger, A. Harvey Millar and Owen K. Atkin

    Plant Methods 2017 13:16

    Published on: 21 March 2017

  4. Methodology

    Development of an efficient glucosinolate extraction method

    Glucosinolates, anionic sulfur rich secondary metabolites, have been extensively studied because of their occurrence in the agriculturally important brassicaceae and their impact on human and animal health. Th...

    T. Doheny-Adams, K. Redeker, V. Kittipol, I. Bancroft and S. E. Hartley

    Plant Methods 2017 13:17

    Published on: 21 March 2017

  5. Methodology

    The Tree Drought Emission MONitor (Tree DEMON), an innovative system for assessing biogenic volatile organic compounds emission from plants

    Biogenic volatile organic compounds (BVOC) emitted by plants play an important role for ecological and physiological processes, for example as response to stressors. These emitted compounds are involved in che...

    Marvin Lüpke, Rainer Steinbrecher, Michael Leuchner and Annette Menzel

    Plant Methods 2017 13:14

    Published on: 20 March 2017

  6. Methodology

    rosettR: protocol and software for seedling area and growth analysis

    Growth is an important parameter to consider when studying the impact of treatments or mutations on plant physiology. Leaf area and growth rates can be estimated efficiently from images of plants, but the expe...

    Filipa Tomé, Karel Jansseune, Bernadette Saey, Jack Grundy, Korneel Vandenbroucke, Matthew A. Hannah and Henning Redestig

    Plant Methods 2017 13:13

    Published on: 15 March 2017

  7. Methodology

    The Microphenotron: a robotic miniaturized plant phenotyping platform with diverse applications in chemical biology

    Chemical genetics provides a powerful alternative to conventional genetics for understanding gene function. However, its application to plants has been limited by the lack of a technology that allows detailed ...

    Thomas Burrell, Susan Fozard, Geoff H. Holroyd, Andrew P. French, Michael P. Pound, Christopher J. Bigley, C. James Taylor and Brian G. Forde

    Plant Methods 2017 13:10

    Published on: 1 March 2017

  8. Research

    A high-throughput stereo-imaging system for quantifying rape leaf traits during the seedling stage

    The fitness of the rape leaf is closely related to its biomass and photosynthesis. The study of leaf traits is significant for improving rape leaf production and optimizing crop management. Canopy structure an...

    Xiong Xiong, Lejun Yu, Wanneng Yang, Meng Liu, Ni Jiang, Di Wu, Guoxing Chen, Lizhong Xiong, Kede Liu and Qian Liu

    Plant Methods 2017 13:7

    Published on: 31 January 2017

  9. Methodology

    Spatially-localized bench-top X-ray scattering reveals tissue-specific microfibril orientation in Moso bamboo

    Biological materials have a complex, hierarchical structure, with vital structural features present at all size scales, from the nanoscale to the macroscale. A method that can connect information at multiple l...

    Patrik Ahvenainen, Patrick G. Dixon, Aki Kallonen, Heikki Suhonen, Lorna J. Gibson and Kirsi Svedström

    Plant Methods 2017 13:5

    Published on: 9 January 2017

  10. Research

    Predicting grain yield using canopy hyperspectral reflectance in wheat breeding data

    Modern agriculture uses hyperspectral cameras to obtain hundreds of reflectance data measured at discrete narrow bands to cover the whole visible light spectrum and part of the infrared and ultraviolet light s...

    Osval A. Montesinos-López, Abelardo Montesinos-López, José Crossa, Gustavo de los Campos, Gregorio Alvarado, Mondal Suchismita, Jessica Rutkoski, Lorena González-Pérez and Juan Burgueño

    Plant Methods 2017 13:4

    Published on: 3 January 2017

  11. Methodology

    The plant leaf movement analyzer (PALMA): a simple tool for the analysis of periodic cotyledon and leaf movement in Arabidopsis thaliana

    The analysis of circadian leaf movement rhythms is a simple yet effective method to study effects of treatments or gene mutations on the circadian clock of plants. Currently, leaf movements are analysed using ...

    Lucas Wagner, Christoph Schmal, Dorothee Staiger and Selahattin Danisman

    Plant Methods 2017 13:2

    Published on: 3 January 2017

  12. Methodology

    Chlorophyll a fluorescence, under half of the adaptive growth-irradiance, for high-throughput sensing of leaf-water deficit in Arabidopsis thaliana accessions

    Non-invasive and high-throughput monitoring of drought in plants from its initiation to visible symptoms is essential to quest drought tolerant varieties. Among the existing methods, chlorophyll a fluorescence (C...

    Kumud B. Mishra, Anamika Mishra, Kateřina Novotná, Barbora Rapantová, Petra Hodaňová, Otmar Urban and Karel Klem

    Plant Methods 2016 12:46

    Published on: 14 November 2016

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