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  1. Methodology

    Spatially-localized bench-top X-ray scattering reveals tissue-specific microfibril orientation in Moso bamboo

    Biological materials have a complex, hierarchical structure, with vital structural features present at all size scales, from the nanoscale to the macroscale. A method that can connect information at multiple l...

    Patrik Ahvenainen, Patrick G. Dixon, Aki Kallonen, Heikki Suhonen, Lorna J. Gibson and Kirsi Svedström

    Plant Methods 2017 13:5

    Published on: 9 January 2017

  2. Research

    Predicting grain yield using canopy hyperspectral reflectance in wheat breeding data

    Modern agriculture uses hyperspectral cameras to obtain hundreds of reflectance data measured at discrete narrow bands to cover the whole visible light spectrum and part of the infrared and ultraviolet light s...

    Osval A. Montesinos-López, Abelardo Montesinos-López, José Crossa, Gustavo de los Campos, Gregorio Alvarado, Mondal Suchismita, Jessica Rutkoski, Lorena González-Pérez and Juan Burgueño

    Plant Methods 2017 13:4

    Published on: 3 January 2017

  3. Methodology

    The plant leaf movement analyzer (PALMA): a simple tool for the analysis of periodic cotyledon and leaf movement in Arabidopsis thaliana

    The analysis of circadian leaf movement rhythms is a simple yet effective method to study effects of treatments or gene mutations on the circadian clock of plants. Currently, leaf movements are analysed using ...

    Lucas Wagner, Christoph Schmal, Dorothee Staiger and Selahattin Danisman

    Plant Methods 2017 13:2

    Published on: 3 January 2017

  4. Methodology

    Chlorophyll a fluorescence, under half of the adaptive growth-irradiance, for high-throughput sensing of leaf-water deficit in Arabidopsis thaliana accessions

    Non-invasive and high-throughput monitoring of drought in plants from its initiation to visible symptoms is essential to quest drought tolerant varieties. Among the existing methods, chlorophyll a fluorescence (C...

    Kumud B. Mishra, Anamika Mishra, Kateřina Novotná, Barbora Rapantová, Petra Hodaňová, Otmar Urban and Karel Klem

    Plant Methods 2016 12:46

    Published on: 14 November 2016

  5. Methodology

    Protocol: a fast, comprehensive and reproducible one-step extraction method for the rapid preparation of polar and semi-polar metabolites, lipids, proteins, starch and cell wall polymers from a single sample

    The elucidation of complex biological systems requires integration of multiple molecular parameters. Accordingly, high throughput methods like transcriptomics, proteomics, metabolomics and lipidomics have emer...

    Mohamed A. Salem, Jessica Jüppner, Krzysztof Bajdzienko and Patrick Giavalisco

    Plant Methods 2016 12:45

    Published on: 10 November 2016

  6. Methodology

    Measures for interoperability of phenotypic data: minimum information requirements and formatting

    Plant phenotypic data shrouds a wealth of information which, when accurately analysed and linked to other data types, brings to light the knowledge about the mechanisms of life. As phenotyping is a field of re...

    Hanna Ćwiek-Kupczyńska, Thomas Altmann, Daniel Arend, Elizabeth Arnaud, Dijun Chen, Guillaume Cornut, Fabio Fiorani, Wojciech Frohmberg, Astrid Junker, Christian Klukas, Matthias Lange, Cezary Mazurek, Anahita Nafissi, Pascal Neveu, Jan van Oeveren, Cyril Pommier…

    Plant Methods 2016 12:44

    Published on: 9 November 2016

  7. Methodology

    UV crosslinked mRNA-binding proteins captured from leaf mesophyll protoplasts

    The complexity of RNA regulation is one of the current frontiers in animal and plant molecular biology research. RNA-binding proteins (RBPs) are characteristically involved in post-transcriptional gene regulat...

    Zhicheng Zhang, Kurt Boonen, Piero Ferrari, Liliane Schoofs, Ewald Janssens, Vera van Noort, Filip Rolland and Koen Geuten

    Plant Methods 2016 12:42

    Published on: 3 November 2016

  8. Research

    Infiltration-RNAseq: transcriptome profiling of Agrobacterium-mediated infiltration of transcription factors to discover gene function and expression networks in plants

    Transcription factors (TFs) coordinate precise gene expression patterns that give rise to distinct phenotypic outputs. The identification of genes and transcriptional networks regulated by a TF often requires ...

    Donna M. Bond, Nick W. Albert, Robyn H. Lee, Gareth B. Gillard, Chris M. Brown, Roger P. Hellens and Richard C. Macknight

    Plant Methods 2016 12:41

    Published on: 19 October 2016

  9. Research

    RADIX: rhizoslide platform allowing high throughput digital image analysis of root system expansion

    Phenotyping of genotype-by-environment interactions in the root-zone is of major importance for crop improvement as the spatial distribution of a plant’s root system is crucial for a plant to access water and ...

    Chantal Le Marié, Norbert Kirchgessner, Patrick Flütsch, Johannes Pfeifer, Achim Walter and Andreas Hund

    Plant Methods 2016 12:40

    Published on: 5 September 2016

  10. Methodology

    A method for detecting single mRNA molecules in Arabidopsis thaliana

    Despite advances in other model organisms, there are currently no techniques to explore cell-to-cell variation and sub-cellular localization of RNA molecules at the single-cell level in plants.

    Susan Duncan, Tjelvar S. G. Olsson, Matthew Hartley, Caroline Dean and Stefanie Rosa

    Plant Methods 2016 12:13

    Published on: 5 August 2016

  11. Methodology

    Development of a universal and simplified ddRAD library preparation approach for SNP discovery and genotyping in angiosperm plants

    The double digest restriction-site associated DNA sequencing technology (ddRAD-seq) is a reduced representation sequencing technology by sampling genome-wide enzyme loci developed on the basis of next-generati...

    Guo-Qian Yang, Yun-Mei Chen, Jin-Peng Wang, Cen Guo, Lei Zhao, Xiao-Yan Wang, Ying Guo, Li Li, De-Zhu Li and Zhen-Hua Guo

    Plant Methods 2016 12:39

    Published on: 4 August 2016

  12. Methodology

    Detecting N-myristoylation and S-acylation of host and pathogen proteins in plants using click chemistry

    The plant plasma membrane is a key battleground in the war between plants and their pathogens. Plants detect the presence of pathogens at the plasma membrane using sensor proteins, many of which are targeted t...

    Patrick C. Boyle, Simon Schwizer, Sarah R. Hind, Christine M. Kraus, Susana De la Torre Diaz, Bin He and Gregory B. Martin

    Plant Methods 2016 12:38

    Published on: 3 August 2016

  13. Methodology

    Application of unmanned aerial systems for high throughput phenotyping of large wheat breeding nurseries

    Low cost unmanned aerial systems (UAS) have great potential for rapid proximal measurements of plants in agriculture. In the context of plant breeding and genetics, current approaches for phenotyping a large n...

    Atena Haghighattalab, Lorena González Pérez, Suchismita Mondal, Daljit Singh, Dale Schinstock, Jessica Rutkoski, Ivan Ortiz-Monasterio, Ravi Prakash Singh, Douglas Goodin and Jesse Poland

    Plant Methods 2016 12:35

    Published on: 24 June 2016

  14. Methodology

    High-throughput phenotyping of lateral expansion and regrowth of spaced Lolium perenne plants using on-field image analysis

    Genetic studies and breeding of agricultural crops frequently involve phenotypic characterization of large collections of genotypes grown in field conditions. These evaluations are typically based on visual ob...

    Peter Lootens, Tom Ruttink, Antje Rohde, Didier Combes, Philippe Barre and Isabel Roldán-Ruiz

    Plant Methods 2016 12:32

    Published on: 10 June 2016

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