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  2. Cowpea (Vigna unguiculata L.) is an important grain and forage legume grown throughout sub-Saharan Africa primarily by subsistence farmers on poor, drought prone soils. Genetic improvement of the crop is being ac...

    Authors: Karolina E Mellor, Ava M Hoffman and Michael P Timko
    Citation: Plant Methods 2012 8:22
    Content type: Methodology Published on:

  4. Striga species are noxious root hemi-parasitic weeds that debilitate cereal production in sub-Saharan Africa (SSA). Control options for Striga are limited and developing Striga resistant crop germplasm is regarde...

    Authors: Steven Runo, Sarah Macharia, Amos Alakonya, Jesse Machuka, Neelima Sinha and Julie Scholes
    Citation: Plant Methods 2012 8:20
    Content type: Methodology Published on:

  5. Information about a transgene locus is one of the major concerns in transgenic research because expression of the transgene or a gene interrupted by the integration event could be affected. Thus, the flanking ...

    Authors: Joung Sug Kim, Jiye Kim, Tae-Ho Lee, Kyong Mi Jun, Tea Hoon Kim, Yul-Ho Kim, Hyang-Mi Park, Jong-Seong Jeon, Gynheung An, Ung-Han Yoon, Baek Hie Nahm and Yeon-Ki Kim
    Citation: Plant Methods 2012 8:19
    Content type: Software Published on:

  6. Microarrays are routine tools for transcript profiling, and genomic tiling arrays such as the Arabidopsis AGRONOMICS1 arrays have been found to be highly suitable for such experiments because changes in genome...

    Authors: Marlen Müller, Andrea Patrignani, Hubert Rehrauer, Wilhelm Gruissem and Lars Hennig
    Citation: Plant Methods 2012 8:18
    Content type: Methodology Published on:

  7. We have developed a new analytical approach for isolation and quantification of cytokinins (CK) in minute amounts of fresh plant material, which combines a simple one-step purification with ultra-high performa...

    Authors: Jana Svačinová, Ondřej Novák, Lenka Plačková, René Lenobel, Josef Holík, Miroslav Strnad and Karel Doležal
    Citation: Plant Methods 2012 8:17
    Content type: Methodology Published on:

  8. Seed transmission constitutes a major component of the parasitic cycle for several fungal pathogens. However, very little is known concerning fungal or plant genetic factors that impact seed transmission and m...

    Authors: Stephanie Pochon, Emmanuel Terrasson, Thomas Guillemette, Beatrice Iacomi-Vasilescu, Sonia Georgeault, Marjorie Juchaux, Romain Berruyer, Isabelle Debeaujon, Philippe Simoneau and Claire Campion
    Citation: Plant Methods 2012 8:16
    Content type: Methodology Published on:

  9. Genetic resources available for Arabidopsis thaliana make this species particularly attractive as a model for molecular genetic studies of guard cell homeostasis, transport and signalling, but this facility is no...

    Authors: Zhong-Hua Chen, Cornelia Eisenach, Xin-Qin Xu, Adrian Hills and Michael R Blatt
    Citation: Plant Methods 2012 8:15
    Content type: Methodology Published on:

  11. We have developed a novel assay based on the ability of type I sucrose uptake transporters (SUTs) to transport the fluorescent coumarin β-glucoside, esculin. Budding yeast (Saccharomyces cerevisiae) is routinely ...

    Authors: Peter J Gora, Anke Reinders and John M Ward
    Citation: Plant Methods 2012 8:13
    Content type: Methodology Published on:

  12. Large-scale genetic screens in Arabidopsis are a powerful approach for molecular dissection of complex signaling networks. However, map-based cloning can be time-consuming or even hampered due to low chromosomal ...

    Authors: Kun-hsiang Liu, Matthew McCormack and Jen Sheen
    Citation: Plant Methods 2012 8:12
    Content type: Methodology Published on:

  13. Accurate quantification of xylem sap ABA concentrations is important to underpin models of root-to-shoot ABA signalling to predict the physiological effects of soil drying. Growing tomato plants in a whole pla...

    Authors: Andrew G Netting, Julian C Theobald and Ian C Dodd
    Citation: Plant Methods 2012 8:11
    Content type: Methodology Published on:

  14. Cytosine methylation is involved in epigenetic control of gene expression in a wide range of organisms. An increasing number of examples indicate that changing the frequency of cytosine methylation in the geno...

    Authors: Sachiko Arase, Megumi Kasai and Akira Kanazawa
    Citation: Plant Methods 2012 8:185
    Content type: Research Published on:

    The Erratum to this article has been published in Plant Methods 2015 11:21

  17. The ability to quantify the geometry of plant organs at the cellular scale can provide novel insights into their structural organization. Hitherto manual methods of measurement provide only very low throughput...

    Authors: Andrew P French, Michael H Wilson, Kim Kenobi, Daniela Dietrich, Ute Voß, Susana Ubeda-Tomás, Tony P Pridmore and Darren M Wells
    Citation: Plant Methods 2012 8:7
    Content type: Software Published on:

  18. Arabidopsis NPR1 is a master regulator of systemic acquired resistance. NPR1 binds to TGA transcription factors and functions as a transcriptional co-activator. In rice, NH1/OsNPR1 functions to enhance innate ...

    Authors: Mawsheng Chern, Wei Bai, Wing Hoi Sze-To, Patrick E Canlas, Laura E Bartley and Pamela C Ronald
    Citation: Plant Methods 2012 8:6
    Content type: Research Published on:

  19. As a result of the growing body of protein phosphorylation sites data, the number of phosphoprotein databases is constantly increasing, and dozens of tools are available for predicting protein phosphorylation ...

    Authors: Shufu Que, Kuan Li, Min Chen, Yongfei Wang, Qiaobin Yang, Wenfeng Zhang, Baoqian Zhang, Bangshu Xiong and Huaqin He
    Citation: Plant Methods 2012 8:5
    Content type: Methodology Published on:

  20. The repeated weekly subculture of plant cell suspension is labour intensive and increases the risk of variation from parental cells lines. Most of the procedures to preserve cultures are based on controlled fr...

    Authors: Anne-Marie Boisson, Elisabeth Gout, Richard Bligny and Corinne Rivasseau
    Citation: Plant Methods 2012 8:4
    Content type: Methodology Published on:

  21. Hyperspectral imaging (HSI) offers high potential as a non-invasive diagnostic tool for disease detection. In this paper leaf characteristics and spectral reflectance of sugar beet leaves diseased with Cercospora

    Authors: Anne-Katrin Mahlein, Ulrike Steiner, Christian Hillnhütter, Heinz-Wilhelm Dehne and Erich-Christian Oerke
    Citation: Plant Methods 2012 8:3
    Content type: Methodology Published on:

  22. Simultaneous analysis of multiple functional-related phytohormones and their metabolites will improve our understanding of interactions among different hormones in the same biologic process.

    Authors: Hongbo Liu, Xianghua Li, Jinghua Xiao and Shiping Wang
    Citation: Plant Methods 2012 8:2
    Content type: Methodology Published on:

  23. Carotenoids are the most widespread group of pigments found in nature. In addition to their role in the physiology of the plant, carotenoids also have nutritional relevance as their incorporation in the human ...

    Authors: Djédoux Maxime Angaman, Rocco Petrizzo, Francesc Hernández-Gras, Carmen Romero-Segura, Irene Pateraki, Montserrat Busquets and Albert Boronat
    Citation: Plant Methods 2012 8:1
    Content type: Methodology Published on:

  24. Rice genome sequencing projects have generated remarkable amount of information about genes and genome architecture having tremendous potential to be utilized in both basic and applied research. Success in tra...

    Authors: Khirod K Sahoo, Amit K Tripathi, Ashwani Pareek, Sudhir K Sopory and Sneh L Singla-Pareek
    Citation: Plant Methods 2011 7:49
    Content type: Methodology Published on:

  25. Proteins in the plant apoplast are essential for many physiological processes. We have analysed and compared six different infiltration solutions for proteins contained in the apoplast to recognize the most su...

    Authors: Katja Witzel, Muhammad Shahzad, Andrea Matros, Hans-Peter Mock and Karl H Mühling
    Citation: Plant Methods 2011 7:48
    Content type: Methodology Published on:

  27. Pea has lagged behind other model legumes in the molecular study of nodulation and mycorrhizae-formation because of the difficulty to transform its roots and its poor growth on agar plates. Here we describe fo...

    Authors: Scott R Clemow, Lindsey Clairmont, Lene H Madsen and Frédérique C Guinel
    Citation: Plant Methods 2011 7:46
    Content type: Methodology Published on:

  28. In Arabidopsis thaliana we demonstrate that dying root hairs provide an easy and rapid in vivo model for the morphological identification of apoptotic-like programmed cell death (AL-PCD) in plants. The model desc...

    Authors: Bridget V Hogg, Joanna Kacprzyk, Elizabeth M Molony, Conor O'Reilly, Thomas F Gallagher, Patrick Gallois and Paul F McCabe
    Citation: Plant Methods 2011 7:45
    Content type: Methodology Published on:

  30. Targeted Induced Loci Lesions IN Genomes (TILLING) is increasingly being used to generate and identify mutations in target genes of crop genomes. TILLING populations of several thousand lines have been generat...

    Authors: Seosamh Ó Lochlainn, Stephen Amoah, Neil S Graham, Khalid Alamer, Juan J Rios, Smita Kurup, Andrew Stoute, John P Hammond, Lars Østergaard, Graham J King, Phillip J White and Martin R Broadley
    Citation: Plant Methods 2011 7:43
    Content type: Methodology Published on:

  32. To simulate expected future global warming, hexagonal arrays of infrared heaters have previously been used to warm open-field canopies of upland crops such as wheat. Through the use of concrete-anchored posts,...

    Authors: Muhammad Ishaq Asif Rehmani, Jingqi Zhang, Ganghua Li, Syed Tahir Ata-Ul-Karim, Shaohua Wang, Bruce A Kimball, Chuan Yan, Zhenghui Liu and Yanfeng Ding
    Citation: Plant Methods 2011 7:41
    Content type: Methodology Published on:

  34. Accurate analysis of DNA methylation by bisulphite sequencing depends on the complete conversion of all cytosines into uracil. Until now there has been no standard or universal gene identified as an endogenous...

    Authors: Jing Wang, Chongnan Wang, Yan Long, Clare Hopkins, Smita Kurup, Kede Liu, Graham J King and Jinling Meng
    Citation: Plant Methods 2011 7:39
    Content type: Methodology Published on:

  35. Complete organellar genome sequences (chloroplasts and mitochondria) provide valuable resources and information for studying plant molecular ecology and evolution. As high-throughput sequencing technology adva...

    Authors: Tongwu Zhang, Xiaowei Zhang, Songnian Hu and Jun Yu
    Citation: Plant Methods 2011 7:38
    Content type: Methodology Published on:

  36. Plant hormones play a pivotal role in several physiological processes during a plant's life cycle, from germination to senescence, and the determination of endogenous concentrations of hormones is essential to...

    Authors: Maren Müller and Sergi Munné-Bosch
    Citation: Plant Methods 2011 7:37
    Content type: Methodology Published on:

  37. Transformation and subsequent regeneration of holoparasitic plants has never been reported, in part due to challenges in developing transformation protocols, but also because regeneration of obligate parasites...

    Authors: Mónica Fernández-Aparicio, Diego Rubiales, Pradeepa CG Bandaranayake, John I Yoder and James H Westwood
    Citation: Plant Methods 2011 7:36
    Content type: Methodology Published on:

  39. To adjust their development to the environment, plants rely on specific signals that travel from shoot to root and vice versa. Here we describe an efficient micrografting protocol for Nicotiana attenuata, a usefu...

    Authors: Variluska Fragoso, Hannah Goddard, Ian T Baldwin and Sang-Gyu Kim
    Citation: Plant Methods 2011 7:34
    Content type: Methodology Published on:

  40. Large-insert BAC and BIBAC libraries are important tools for structural and functional genomics studies of eukaryotic genomes. To facilitate the construction of BAC and BIBAC libraries and the transfer of comp...

    Authors: Xue Shi, Haiyang Zeng, Yadong Xue and Meizhong Luo
    Citation: Plant Methods 2011 7:33
    Content type: Methodology Published on:

  41. The Arabidopsis thaliana-Pseudomonas syringae model pathosystem is one of the most widely used systems to understand the mechanisms of microbial pathogenesis and plant innate immunity. Several inoculation methods...

    Authors: Yasuhiro Ishiga, Takako Ishiga, Srinivasa R Uppalapati and Kirankumar S Mysore
    Citation: Plant Methods 2011 7:32
    Content type: Methodology Published on:

  42. Microarray technology has matured over the past fifteen years into a cost-effective solution with established data analysis protocols for global gene expression profiling. The Agilent-016047 maize 44 K microar...

    Authors: Nanette Coetzer, Alexander A Myburg and Dave K Berger
    Citation: Plant Methods 2011 7:31
    Content type: Database Published on:

  43. Plant protoplasts, a proven physiological and versatile cell system, are widely used in high-throughput analysis and functional characterization of genes. Green protoplasts have been successfully used in inves...

    Authors: Yang Zhang, Jianbin Su, Shan Duan, Ying Ao, Jinran Dai, Jun Liu, Peng Wang, Yuge Li, Bing Liu, Dongru Feng, Jinfa Wang and Hongbin Wang
    Citation: Plant Methods 2011 7:30
    Content type: Methodology Published on:

  44. Plastids are small organelles equipped with their own genomes (plastomes). Although these organelles are involved in numerous plant metabolic pathways, current knowledge about the transcriptional activity of p...

    Authors: Agnieszka Żmieńko, Magdalena Guzowska-Nowowiejska, Radosław Urbaniak, Wojciech Pląder, Piotr Formanowicz and Marek Figlerowicz
    Citation: Plant Methods 2011 7:29
    Content type: Methodology Published on:

  45. Wheat is one of the most important crops in Australia, and the identification of young plants is an important step towards developing an automated system for monitoring crop establishment and also for differen...

    Authors: Mahmood R Golzarian and Ross A Frick
    Citation: Plant Methods 2011 7:28
    Content type: Methodology Published on:

  46. Total internal reflection fluorescence microscopy (TIRFM) is a powerful tool for observing fluorescently labeled molecules on the plasma membrane surface of animal cells. However, the utility of TIRFM in plant...

    Authors: Yinglang Wan, William M Ash III, Lusheng Fan, Huaiqin Hao, Myung K Kim and Jinxing Lin
    Citation: Plant Methods 2011 7:27
    Content type: Methodology Published on:

  47. We outline a high throughput procedure that improves outlier detection in cell wall screens using FT-NIR spectroscopy of plant leaves. The improvement relies on generating a calibration set from a subset of a ...

    Authors: Andreia M Smith-Moritz, Mawsheng Chern, Jeemeng Lao, Wing Hoi Sze-To, Joshua L Heazlewood, Pamela C Ronald and Miguel E Vega-Sánchez
    Citation: Plant Methods 2011 7:26
    Content type: Methodology Published on:

  48. Transient expression of proteins in plants has become a favoured method over the production of stably transformed plants because, in addition to enabling high protein yields, it is both fast and easy to apply....

    Authors: Dirk Stephan, Coba Slabber, Gavin George, Victor Ninov, Kevin P Francis and Johan T Burger
    Citation: Plant Methods 2011 7:25
    Content type: Methodology Published on:

  49. A method was developed to identify insertional mutants of Chlamydomonas reinhardtii disrupted for selected target genes. The approach relies on the generation of thousands of transformants followed by PCR-based s...

    Authors: David Gonzalez-Ballester, Wirulda Pootakham, Florence Mus, Wenqiang Yang, Claudia Catalanotti, Leonardo Magneschi, Amaury de Montaigu, Jose J Higuera, Matthew Prior, Aurora Galván, Emilio Fernandez and Arthur R Grossman
    Citation: Plant Methods 2011 7:24
    Content type: Methodology Published on:

  50. Prenylquinones are key compounds of the thylakoid membranes in chloroplasts. To understand the mechanisms involved in the response of plants to changing conditions such as high light intensity, the comprehensi...

    Authors: Jacopo Martinis, Felix Kessler and Gaetan Glauser
    Citation: Plant Methods 2011 7:23
    Content type: Methodology Published on:
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Plant Methods

ISSN: 1746-4811