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  1. Trehalose is a non-reducing disaccharide that is used as an osmolyte, transport sugar, carbon reserve and stress protectant in a wide range of organisms. In plants, trehalose 6-phosphate (Tre6P), the intermedi...

    Authors: Petronia Carillo, Regina Feil, Yves Gibon, Namiko Satoh-Nagasawa, David Jackson, Oliver E Bläsing, Mark Stitt and John Edward Lunn
    Citation: Plant Methods 2013 9:21
  2. Nectar reabsorption is a widely known phenomenon, related to the strategy of resource-recovery and also to maintain the nectar homeostasis at the nectary. The method currently performed to demonstrate nectar b...

    Authors: Poliana Cardoso-Gustavson, João Marcelo Robazzi Bignelli Valente Aguiar, Emerson Ricardo Pansarin and Fábio de Barros
    Citation: Plant Methods 2013 9:20
  3. When conducting plant research, the measurement of photosynthetic pigments can provide basic information on the physiological status of a plant. High-pressure liquid chromatography (HPLC) is becoming widely us...

    Authors: Xueyun Hu, Ayumi Tanaka and Ryouichi Tanaka
    Citation: Plant Methods 2013 9:19
  4. When conducting aphid stylectomy, measuring accurate rates of phloem exudation is difficult because the volumes collected are in the nanolitre (nl) range. In a new method, exudate volume was calculated from op...

    Authors: Lachlan J Palmer, Lyndon T Palmer, Jeremy Pritchard, Robin D Graham and James CR Stangoulis
    Citation: Plant Methods 2013 9:18
  5. In order to select for quantitative plant resistance to pathogens, high throughput approaches that can precisely quantify disease severity are needed. Automation and use of calibrated image analysis should pro...

    Authors: Céline Rousseau, Etienne Belin, Edouard Bove, David Rousseau, Frédéric Fabre, Romain Berruyer, Jacky Guillaumès, Charles Manceau, Marie-Agnès Jacques and Tristan Boureau
    Citation: Plant Methods 2013 9:17
  6. Plant extracts are a reservoir of pharmacologically active substances; however, conventional analytical methods can analyze only a small portion of an extract. Here, we report a high-throughput analytical meth...

    Authors: Kyu Hwan Park, Min Sun Kim, Sun Jong Baek, Ik Hyun Bae, Sang-Wan Seo, Jongjin Kim, Yong Kook Shin, Yong-Moon Lee and Hyun Sik Kim
    Citation: Plant Methods 2013 9:15
  7. Grafting procedures are an excellent tool to study long range signalling processes within a plant. In the last decade, suitable flat-surface grafting procedures for young Arabidopsis seedlings using a collar t...

    Authors: Nayelli Marsch-Martínez, John Franken, Karla L Gonzalez-Aguilera, Stefan de Folter, Gerco Angenent and Elena R Alvarez-Buylla
    Citation: Plant Methods 2013 9:14
  8. Brassinosteriods (BRs), a group of important phytohormones, have various effects on plant growth and development. However, their physiological functions in plants have not been fully understood to date. Endoge...

    Authors: Jun Ding, Li-Jing Mao, Bi-Feng Yuan and Yu-Qi Feng
    Citation: Plant Methods 2013 9:13
  9. The hydraulic conductivity of the stem is a major factor limiting the capability of trees to transport water from the soil to transpiring leaves. During drought conditions, the conducting capacity of xylem can...

    Authors: Jussi-Petteri Suuronen, Marko Peura, Kurt Fagerstedt and Ritva Serimaa
    Citation: Plant Methods 2013 9:11
  10. X-ray micro-Computed Tomography (μCT) offers the ability to visualise the three-dimensional structure of plant roots growing in their natural environment – soil. Recovery of root architecture descriptions from...

    Authors: Stefan Mairhofer, Susan Zappala, Saoirse Tracy, Craig Sturrock, Malcolm John Bennett, Sacha Jon Mooney and Tony Paul Pridmore
    Citation: Plant Methods 2013 9:8
  11. Heat stress and heat damage to plants gain globally increasing importance for crop production and plant survival in endangered habitats. Therefore the knowledge of heat tolerance of plants is of great interest...

    Authors: Othmar Buchner, Matthias Karadar, Ines Bauer and Gilbert Neuner
    Citation: Plant Methods 2013 9:7
  12. Public genomic databases have provided new directions for molecular marker development and initiated a shift in the types of PCR-based techniques commonly used in plant science. Alongside commonly used arbitra...

    Authors: Péter Poczai, Ildikó Varga, Maarja Laos, András Cseh, Neil Bell, Jari PT Valkonen and Jaakko Hyvönen
    Citation: Plant Methods 2013 9:6
  13. There are a number of unresolved issues in the design of experiments in greenhouses. They include whether statistical designs should be used and, if so, which designs should be used. Also, are there thigmomorp...

    Authors: Chris J Brien, Bettina Berger, Huwaida Rabie and Mark Tester
    Citation: Plant Methods 2013 9:5
  14. Hydroponic growth systems are a convenient platform for studying whole plant physiology. However, we found through trialling systems as they are described in the literature that our experiments were frequently...

    Authors: Simon J Conn, Bradleigh Hocking, Maclin Dayod, Bo Xu, Asmini Athman, Sam Henderson, Lucy Aukett, Vanessa Conn, Monique K Shearer, Sigfredo Fuentes, Stephen D Tyerman and Matthew Gilliham
    Citation: Plant Methods 2013 9:4
  15. Rhizo-lysimeters offer unique advantages for the study of plants and their interactions with soils. In this paper, an existing facility at Charles Sturt University in Wagga Wagga Australia is described in deta...

    Authors: Philip L Eberbach, Jeffrey Hoffmann, Sergio J Moroni, Leonard J Wade and Leslie A Weston
    Citation: Plant Methods 2013 9:3
  16. Root growth is highly responsive to temporal changes in the environment. On the contrary, diel (24 h) leaf expansion in dicot plants is governed by endogenous control and therefore its temporal pattern does no...

    Authors: Tom Ruts, Shizue Matsubara and Achim Walter
    Citation: Plant Methods 2013 9:2
  17. Plant grafting techniques have deepened our understanding of the signals facilitating communication between the root and shoot, as well as between shoot and reproductive organs. Transmissible signalling molecu...

    Authors: Nazia Nisar, Shelley Verma, Barry J Pogson and Christopher I Cazzonelli
    Citation: Plant Methods 2012 8:50
  18. Quantification of callose deposits is a useful measure for the activities of plant immunity and pathogen growth by fluorescence imaging. For robust scoring of differences, this normally requires many technical...

    Authors: Ji Zhou, Thomas Spallek, Christine Faulkner and Silke Robatzek
    Citation: Plant Methods 2012 8:49
  19. In this report we describe a chromatin immunoprecipitation (ChIP) protocol for two fully sequenced model diatom species Phaeodactylum tricornutum and Thalassiosira pseudonana. This protocol allows the extraction ...

    Authors: Xin Lin, Leïla Tirichine and Chris Bowler
    Citation: Plant Methods 2012 8:48
  20. Phytohormones are the key metabolites participating in the regulation of multiple functions of plant organism. Among them, jasmonates, as well as abscisic and salicylic acids are responsible for triggering and...

    Authors: Gerd Ulrich Balcke, Vinzenz Handrick, Nick Bergau, Mandy Fichtner, Anja Henning, Hagen Stellmach, Alain Tissier, Bettina Hause and Andrej Frolov
    Citation: Plant Methods 2012 8:47
  21. Accurate characterization of complex plant phenotypes is critical to assigning biological functions to genes through forward or reverse genetics. It can also be vital in determining the effect of a treatment, ...

    Authors: Jason M Green, Heidi Appel, Erin MacNeal Rehrig, Jaturon Harnsomburana, Jia-Fu Chang, Peter Balint-Kurti and Chi-Ren Shyu
    Citation: Plant Methods 2012 8:45
  22. The male germline in flowering plants differentiates by asymmetric division of haploid uninucleated microspores, giving rise to a vegetative cell enclosing a smaller generative cell, which eventually undergoes...

    Authors: Filipe Borges, Rui Gardner, Telma Lopes, Joseph P Calarco, Leonor C Boavida, R Keith Slotkin, Robert A Martienssen and Jörg D Becker
    Citation: Plant Methods 2012 8:44
  23. A large number of different plant lines are produced and maintained in a typical plant research laboratory, both as seed stocks and in active growth. These collections need careful and consistent management to...

    Authors: Jeroen Nieuwland, Emily Sornay, Angela Marchbank, Barend HJ de Graaf and James AH Murray
    Citation: Plant Methods 2012 8:43
  24. Cytokinins (CKs) are a group of plant growth regulators that are involved in several plant developmental processes. Despite the breadth of knowledge surrounding CKs and their diverse functions, much remains to...

    Authors: Scott C Farrow and RJ Neil Emery
    Citation: Plant Methods 2012 8:42
  25. Deep sequencing is a powerful tool for novel small RNA discovery. Illumina small RNA sequencing library preparation requires a pre-adenylated 3’ end adapter containing a 5’,5’-adenyl pyrophosphoryl moiety. In ...

    Authors: Yun-Ru Chen, Yi Zheng, Bao Liu, Silin Zhong, Jim Giovannoni and Zhangjun Fei
    Citation: Plant Methods 2012 8:41
  26. Some prominent cultured plant cell lines, such as the BY-2 cell line of tobacco (Nicotiana tabacum cv. ‘Bright Yellow 2’) and the T87 cell line of Arabidopsis (Arabidopsis thaliana L. Heynh., ecotype Columbia) ar...

    Authors: Shinjiro Ogita, Taiji Nomura, Takao Kishimoto and Yasuo Kato
    Citation: Plant Methods 2012 8:40
  27. Phytophthora cinnamomi Rands. is an important root rot pathogen widely distributed in the north hemisphere, with a large host range. Among others diseases, it is known to be a principal factor in the decline of h...

    Authors: Francisco J Ruiz-Gómez, Rafael Sánchez-Cuesta, Rafael M Navarro-Cerrillo and Alejandro Pérez-de-Luque
    Citation: Plant Methods 2012 8:39
  28. Long distance signaling is a common phenomenon in animal and plant development. In plants, lateral organs such as nodules and lateral roots are developmentally regulated by root-to-shoot and shoot-to-root long...

    Authors: Tessema K Kassaw and Julia A Frugoli
    Citation: Plant Methods 2012 8:38
  29. Starch is the most important source of calories for human nutrition and the majority of it is produced by cereal farming. Starch is also used as a renewable raw material in a range of industrial sectors. It ca...

    Authors: Massimiliano Carciofi, Andreas Blennow, Morten M Nielsen, Preben B Holm and Kim H Hebelstrup
    Citation: Plant Methods 2012 8:36
  30. Although Single Nucleotide Polymorphism (SNP) marker is an invaluable tool for positional cloning, association study and evolutionary analysis, low SNP detection efficiency by Allele-Specific PCR (AS-PCR) stil...

    Authors: Jing Liu, Shunmou Huang, Meiyu Sun, Shengyi Liu, Yumei Liu, Wanxing Wang, Xiurong Zhang, Hanzhong Wang and Wei Hua
    Citation: Plant Methods 2012 8:34
  31. The plant hormone auxin, indole-3-acetic acid (IAA), plays important roles in plant growth and development. The signaling response to IAA is largely dependent on the local concentration of IAA, and this concen...

    Authors: Xing Liu, Adrian D Hegeman, Gary Gardner and Jerry D Cohen
    Citation: Plant Methods 2012 8:31
  32. Quantification of leaf movement is an important tool for characterising the effects of environmental signals and the circadian clock on plant development. Analysis of leaf movement is currently restricted by t...

    Authors: Ralph Bours, Manickam Muthuraman, Harro Bouwmeester and Alexander van der Krol
    Citation: Plant Methods 2012 8:29
  33. Sterols and Sphingolipids form lipid clusters in the plasma membranes of cell types throughout the animal and plant kingdoms. These lipid domains provide a medium for protein signaling complexes at the plasma ...

    Authors: Jörg O Blachutzik, Fatih Demir, Ines Kreuzer, Rainer Hedrich and Gregory S Harms
    Citation: Plant Methods 2012 8:28
  34. Understanding protein and gene function requires identifying interaction partners using biochemical, molecular or genetic tools. In plants, searching for novel protein-protein interactions is limited to protei...

    Authors: Kenneth Wayne Berendzen, Maik Böhmer, Niklas Wallmeroth, Sébastien Peter, Marko Vesić, Ying Zhou, Franziska KatharinaElisabeth Tiesler, Frank Schleifenbaum and Klaus Harter
    Citation: Plant Methods 2012 8:25
  35. Recent progress in cassava transformation has allowed the robust production of transgenic cassava even under suboptimal plant tissue culture conditions. The transformation protocol has so far been used mostly ...

    Authors: Ima M Zainuddin, Kim Schlegel, Wilhelm Gruissem and Hervé Vanderschuren
    Citation: Plant Methods 2012 8:24
  36. Cowpea (Vigna unguiculata L.) is an important grain and forage legume grown throughout sub-Saharan Africa primarily by subsistence farmers on poor, drought prone soils. Genetic improvement of the crop is being ac...

    Authors: Karolina E Mellor, Ava M Hoffman and Michael P Timko
    Citation: Plant Methods 2012 8:22

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