Dissecting organ-specific transcriptomes through RNA-sequencing
© Guan and Lu; licensee BioMed Central Ltd. 2013
Received: 2 September 2013
Accepted: 21 October 2013
Published: 25 October 2013
Organ-specific gene expression contains rich information about in vivo biological processes. This kind of information, previously gathered through microarray profiling, has been proven fruitful to the understanding of specific mutants, regulatory events, signaling, and development. With the advent of the next-generation sequencing (NGS) of RNAs, more quantitative and detailed information of gene expressions than previously available can now be collected for each organ or organ developmental stages. The combination of an object-oriented experimental design and an efficient treatment of the high volume information generated through a NGS platform may offer a powerful tool for inferring previously intractable developmental processes.
We collected transcriptomic data over a Solexa/Illumina platform on samples of Ipomoea leaf, sepal, and petals (at three developmental stages), and presented a method for analyzing transcriptomic variations within and between organs. We demonstrated that in vivo signals of transcriptomes can be retrieved de novo through the NGS techniques, proper data handling, bioinformatic tools, and the current understanding of molecular networks. We found that numbers of transcribed genes from both nuclear and chloroplast genomes decreased by the same order of leaf → sepal →petal. Petal resembled leaf in cell division patterns and abundance level of commonly expressed organelle genes. Its chloroplast transcripts constituted a subset of those in leaf. Moreover, reconstructions of multiple metabolic networks for each organ enabled inferences of substance flow, providing transcript evidence for the path of sucrose in leaf to anthocyanin synthesis in petal.
Our results attest that developmental transcriptomes are highly informative for exploring connections between morphological traits and the associated molecular networks. Significant hypotheses have been developed, including that the petal is a derived organ of leaf and that its color can be modified by fluctuations of substance flow within the associated metabolic networks among organs.
Organ differentiation in multi-cellular organisms depends on highly organized molecular events and brings enhanced functionalities to the organisms for their adaptations to natural environments. This differentiation appears to be closely linked to genomic gene expression, as organs contribute most significantly to gene expression variation when compared to all other experimental variables . Organs are connected by substance flow to maintain their overall functions, but the details of which have yet to be made clear at cellular and molecular levels. Knowing these details has significant bearings on understanding how molecular systems control cellular activities and associated phenotypes. Although a significant spot light has been given to gene regulation in interpreting developmental and phenotypic variation [2–4], much is yet to be known on how such gene regulation is realized on the genomic scale and what information is conveyed between cells and organs to keep the integrity of a genet. Here, we focus on the logic and ways of extracting information from sequence-based transcriptomes to shed light on relevant issues.
Key issues on organ functionality include how substance flow connects organs and how the flow is regulated at the whole plant level. These issues concern numerous biological processes at cellular and organ levels, requesting solutions of genomic scope. A genome-based transcriptome has been known to be sensitive to both developmental and environmental signals [5, 6], thus is a natural starting point for explorations of organ-specific expressions. The collective gene expressions may provide information at two levels. One is an in vivo profile of transcript abundances of the living cells, and the other is a snapshot of genomic regulation. These kinds of information are highly useful in the light of recent findings that significant correlations exist between transcriptomic and protein profiles in roots of Arabidopsis and human stem cells . At least in these cases, it is possible to trace back dynamics of cellular and molecular activities to changes in transcriptomes. In a large picture, the correlations are expected to be held broadly in living cells, because they allow genomic regulations to be effective without involving other mechanisms for many molecular systems. Even in cases of post-transcriptional or post-translational modifications, knowing transcript dynamics is still a prerequisite for analysis of the modifications. Systematic analysis of transcriptomes may therefore yield useful inferences on both venues and mechanisms of various molecular systems in cells.
Technically, new biotechnologies such as the next generation sequencing (NGS) have dramatically increased the likelihood of obtaining in vivo information embedded in cells. The most cost-effective platforms in NGS are ones capable of producing short-read sequences in high throughputs and at an affordable price [9, 10]. The reliability of the NGS data has been confirmed in several cases , but analysis of genomic expression patterns awaits further improvements. Obviously, an effective analysis of transcriptomes critically replies on both the sampling scheme and the quality of transcriptome reconstructions. We show that it is attainable now to carry out quantitative analysis of transcriptomes collected at cell and organ levels with NGS short-read assembly methods [12–14]. More significantly, the transcriptomic reconstruction can be performed in a species without genome reference. The de novo approach is highly attractive for genetically less studied or refractory species, potentially accelerating scientific discoveries.
Examples are given here to illustrate how to use the de novo approach to address issues on cellular activities and substance flow within a developing organ and between organs. Three organs - leaf, sepal, and petal - in the common morning glory (Ipomoea purpurea) are considered here, and an experiment is designed to focus on petal developmental stages, particularly the onset and progressive anthocyanin synthesis in the corolla. The transcriptomes of the sepal and the leaf on the same genet serve as comparison bases for analysis of the petal transcriptomes. By reconstructing developmental and organ-specific transcriptomes and connecting well-built transcriptomes to the current knowledge on cellular/molecular activities, we are able to reveal unprecedented details on cellular dynamics during the petal development, providing concrete evidence for inferring substance flow between organs and within petal developmental stages. The inferences shed much light on the origin of petals, while providing a rich context in which different molecular systems are interconnected and regulated to render specific functions.
Results and discussion
Transcriptomic reconstruction, classification, evaluation, and quantification
Results of Solexa/Illumina paired-end reads (75nt in length) assembled by Trinity
Number of raw reads
Number of contig entries
% Reads usage
We evaluated the quality of the transcriptomic assemblies by independent means. The accuracy of the final assembly was examined via cDNA cloning and sequencing of several commonly used genes such as ones encoding GAPDH2 (JN882353), SK (JQ256515), DAHPS (JQ256519), and ACTIN4 (JN882352), and of metabolic network genes such as those encoding PAL (KC794954), C4H (KC794955), 4CL (KC794953), CHS-D (AF358655), CHI (AF028238), DFR-B (U90432), F3H (U74081), F3'H (EU032626), ANS (EU032612), 3GT (EU032615), 3GGT (KC794956), MYB1 (AB232769), bHLH2 (EU032619), and WDR1(EU032621) in the flavonoid network. In all cases, the sequences amplified via RT-PCRs matched nearly perfectly to the final assembly.
To quantitatively compare the assemblies, we adapted a normalized procedure of Robinson and Oshlack . In comparison to previous data handlings such as reads per kilobase per million mapped (RPKM) and quantile normalization , the normalization considers not only the relationship between read number and gene length but also sample characters. It does so by taking the common set of genes across samples as the base for the normalization, effectively excluding transcripts selectively expressed in each organ and the associated bias (Additional file 1). To make sure that the normalized gene expressions still reflected sampled tissues, we took independent samples of petals at the same stage (sample D) on the same genet, and collected data on transcript estimates of ten genes on the anthocyanin pathway through the conventional real-time quantitative PCRs. We observed a significant correlation (Pearson correlation coefficient = 0.77, P < 0.05, t-test, n = 10) between the two sets of data (Additional file 2), and conclude that the normalization procedure did not cause significant shifts of patterns among samples.
Comparisons of subcellular classifications in scaffold number and expression level (normalized in parentheses) among leaf, sepal, and corolla (=limb + tube) transcriptomes of Ipomoea purpurea
Gene usages of chloroplastic, mitochondrial, and nuclear genomes
We found that organ-specific gene expressions made up less than 50% of the total transcripts for each of the subcellular genomes. Relatively speaking, leaf expressed 59% more chloroplastic genes and 16% more nuclear genes than the average of petals, presumably to support cellular activities of the whole plant; petal at a later developmental stage consumed a high volume of mitochondrial transcripts to assist its function; sepal engaged the least mitochondrial genes, but shared features with both petals and leaves in other expression categories (Table 2). Significantly, the nuclear transcriptome generated 518 petal-specific scaffolds, implying a major role of nuclear genes in petal diversification (Figure 3C). The chloroplast genome showed the most striking diversification in both transcribed gene number and expression level (Figure 3D).
When regarding petal and sepal as representative parts of flower, we observed a significantly different nuclear gene usage between leaf and flower (Wilcoxon rank test, P = 0.03). The same significant differences were also seen for chloroplastic gene usage and the average transcript abundance between leaf and flower. Furthermore, a sequential reduction of transcribed gene number was found in the order of leaf → sepal → petal for both nuclear and chloroplastic genomes. This pattern is noteworthy since it appears to connect to one in Arabidopsis, where pollen exhibited a reduction of about 1/3 of commonly transcribed genes relative to vegetative organs . Also during senescence, the transcriptomic profile of Arabidopsis silique is more similar to that of its petal than that of the leaf . Whether or not this trend of a reduced genomic usage toward the center of a flower is ubiquitous requires additional data. The kind of information has significant bearings. One is on deciphering evolutionary histories of the organs. Floral developmental program has been considered to share a deep homology in angiosperms , however, the emergence of petals in land plants is still an enigma  despite of examinations from multiple angles [33, 34]. The similarities found here between petal and leaf in chloroplast gene usage and expression patterns of common transcripts suggests a deep connection between petal and leaf.
Our data further shows that petals are partially autotrophic and costly to maintain. During petal development, two photosystem I genes (ABCDEF_39040 encoding psaI and ABCDEF_49598 encoding psaA) expressed only temporarily after the petal's exposure to light, coinciding with the temporary greening of petals prior to blooming (Figure 1). During the petal pigmentation period as seen in samples A, C, and D, ribosomal genes (e.g., ABCDEF_17426, ABCDEF_41509) of the mitochondrial genome enhanced their expressions, and ATP synthase transcripts (ABCDEF_38143) increased nearly three folds. These patterns indicate that gene usages are dynamic in subcellular genomes, which may vary by genome type, organ type, the developmental stage of an organ, and the environment.
Cellular dynamics depicted by transcript abundance levels
The known networks depicted by the Kyoto encyclopedia of genes and genomes (KEGG) database provide a basic framework for reconstructing molecular pathways. To link gene expressions to metabolites, we mapped all annotated entries of the final assembly to KEGG (Additional file 5). We made two assumptions while making our inferences. One is a positive correlation between transcript abundance and protein expression level; the other is one between protein abundance and enzyme activity. The first correlation has gained support from tissue or cell-specific studies [7, 8, 35]. The second one can be derived from Michaelis-Menten kinetics, which suggests that richness of an enzyme (E) is positively correlated with the enzyme's activity until the substrate (S) is exhausted in forming ES. Both types of correlation may hold broadly.
Gene expression patterns of petal cell division
Active RNA synthesis and translation in petals
Epigenetic events differ between organs
The current understanding of epigenetic processes permits preliminary comparisons of some components across organs. These components diverged at both DNA and protein levels, suggesting that epigenetic processes are likely to be among the key events for organ differentiation (Additional file 6). In petal, we observed a highly expressed homolog (ABCDEF_38770) of ARGONAUTE 1 (AGO1), along with a Dicer homolog (ABCDEF_40338). AGO1 participates in single-stranded RNA cleavage via microRNA , while Dicer is known to cut double-stranded RNAs to influence DNA methylation status . Their expression patterns suggest different dynamics of small RNA processes between organs. In a similar pattern, genes for histone deacetylases (e.g., ABCDEF_42471) were more actively transcribed in petals while protein phosphotase 2C gene (ABCDEF_3211) was expressed more in leaves and sepals. For the colored limb (Figure 1), transcripts (ABCDEF_4131) highly resembling a maize AC transposase gene  were about 4–8 folds higher than those in leaves and sepals. It appears that different epigenetic strategies have been taken between organs.
Connecting metabolic pathways to infer substance flow
As metabolic processes are the basis for the substance flow among organs, they are compared among organs and organ developmental stages to extract information. We organized the annotated final transcriptome by the classification of KEGG (Additional file 5) and compared the file against known network topologies to establish data-supported networks. As indicated above, over 50% of transcriptomic components are shared among organs, and many of the known metabolic networks can be compared at a transcript level across samples. To make inferences on substance flow, we mapped the in vivo transcript accumulation patterns across the six samples along metabolic networks using sign and color. Examples are given here focusing on glycolysis, amino acid pathways, and synthetic pathways to flavonoids and terpenoids, since our experimental design particularly targets petal pigmentation process (Figure 1). Anthocyanins are known to be the major pigments in the petal of Ipomoea. Our initial concern was to know how the anthocyanin pathway had been related to other metabolic processes in vivo.
Reconstruction of metabolic networks via organ transcriptomes
To seek the connection between glycolysis and other networks, we extended the network reconstruction around glycolysis, and recruited acetyl-CoA carboxylase (EC 188.8.131.52). This enzyme requires one ATP for synthesizing one malonyl-CoA from one acetyl-CoA, while malonyl-CoA is one of the precursors for anthocyanins. We found that the transcripts of acetyl-CoA carboxylase increased about 4-fold between samples A and D. As a result, an enhanced substance flow was channeled into production of malonyl-CoA during petal pigmentation via this linking enzyme between glycolysis and flavonoid synthesis (Figure 6A). This also answered to some extent why much energy was consumed in petals.
Since anthocyanin pigments are known to be from three malonyl-CoA and one 4-coumaroyl-CoA  by the catalytic reaction of chalcone synthase (CHS), we further explored the in vivo source of 4-coumaroyl-CoA in petal. The mapping of amino acid biosynthesis (Figure 6B) suggested that it came directly from phenylalanine metabolism (KEGG #00360) via highly expressed transcripts for phenylalanine ammonia-lyase (184.108.40.206). The enzyme had two types of isoforms in samples. Both increased along with petal development. In comparisons to their counterparts in leaf and sepal, the shorter one increased about ten folds in tube, and the longer ones boosted their levels by 2–4 folds in colored limbs. Since the floral tube of Ipomoea fuses with the anther filaments, it is difficult to make a judgment on the significance of these alternative transcripts. For our purpose of inferring substance flow, these expression patterns suggested a continuous substance flow from the branch of phenylalanine synthesis to the in vivo production of 4-coumaroyl-CoA during development of limb pigmentation, and the substance flow could be stronger in the floral tube.
Inferring substance flow between organs
The identification of the connections between glycolysis and the anthocyanin pathway was significant as it allowed deciphering of extended substance flow involving the supply of glucose in petal. By reconstructing the network involving starch and sucrose (KEGG #00500), we noticed that relative to leaf, petal had a 4-fold higher transcript level for alpha-glucosidase (EC220.127.116.11, ABCDEF_11272) in samples D&E and 18-fold higher transcripts for beta-glucosidase (EC18.104.22.168, ABCDEF_52515) in sample E alone. These enzymes could take different forms of glucoside to generate glucose to meet the high demand in petals. The final anthocyanin molecule in Ipomoea can be added with five glucoses to maintain its stability in vacuole [46, 50]. Moreover, sucrose from leaf could replenish glucose in petal when the demand of glucose in petal outpaced the local supply. The evidence for this scenario came from the increasing patterns (2- to 4-fold) of homolog transcripts (ABCDEF_25171, ABCDEF_41696) of two sucrose transporter genes (SUC1 and SUC2) in the petal developmental series. The homolog expression of beta-fructofuranosidase (EC22.214.171.124, ABCDEF_35900) was most informative, as it increased over three magnitudes in samples D&E versus the leaf sample. This enzyme holds a 64% identity to the vacuolar form of acid invertase in bean (Vicia faba), which is known to convert sucrose into glucose and fructofuranose. It is possible that leaf-produced sucrose can be transported via the vascular systems in floral tube to satisfy the need of glucose during petal pigmentation process. Although the concrete molecular path connecting different tissues still requires characterizations, externally supplied sucrose have been known to enhance anthocyanin accumulation in Eustoma and Arabidopsis[52, 53]. The in vivo substance flow detected in the petal series indicates how floral color can be altered by fluctuation of upstream metabolites.
Reconstructing signaling networks
GA synthesis in sepals, however, appeared to follow a different path of substance flow---the transcript level of 5-epi-aristolochene synthase (EC 126.96.36.199, ABCDEF_11886, 59% identity to NaEAS37) increased a 24-fold relative to petals and about three folds relative to leaves (Figure 8). This may cause a high accumulation of a capsidiol-like phytoalexin in sepal. As casbene can reduce fungal infection , a likely high accumulation in sepal may provide a general mechanism for the protective role of sepal during developments of floral buds and young fruits. The phenomenon certainly deserves more close examinations.
Since transcriptomic reconstruction may provide rich information on cellular activities and substance flow in organs (or cells in cases of microscopic samples), it should be an integral part of biological investigations in future. We provide here the logic and ways of extracting transcriptome information. Cellular activities may be largely reconstructed from patterns of genomic usages and transcript abundance among samples under an object-oriented experimental design. Inferences may be drawn from known topology of networks and their validated components. Our analysis shows that while leaf and sepal are both photosynthetic, leaf is an essential organ for energy capturing and primary metabolism, as more genes were transcribed in higher quantities than those in sepal. Though photosynthetic and possibly autotropic to some degree, sepal has been specialized in other functions such as protection by accumulating organ-specific secondary compounds such as phytoalexins. Petal pigmentation process can be visualized step-by-step from glycolysis to anthocyanin pathway by linking relevant KEGG metabolic topologies via known biochemical reactions. The continuity in substance flow is therefore clearly visible between the primary and secondary metabolic networks. A less continuous image, however, also emerged in our analysis, showing that sucrose could be transported from leaf to petal to replenish glucose during petal pigmentation process. While the missing links between molecular systems still depend on future laboratory discoveries, the integration of NGS technique, bioinformatic analysis, and an appropriate experimental design may certainly help to orient the search directions to more likely domains.
Sampling scheme and data collection
Six samples of Ipomoea purpurea were taken on 25th September 2009 from a healthy individual of a selfing line (III6Da) grown naturally at the Botanical Garden of the Institute of Botany, CAS, at Beijing. Young leaves, sepals, and petals (Figure 1) were frozen immediately in liquid nitrogen and the whole RNAs were subsequently extracted using Trizol reagent (Life Technologies). The quality of the extracted RNAs was examined with Agilent 2100 Bioanalyzer (Agilent Technologies) and the samples were processed following the sample preparation guide for mRNA sequencing (Illumina 2009). The preparation involved purification of mRNAs by poly-T oligos attached to magnetic beads, and cDNA synthesis in two strands. The cDNAs were added with A bases at 3' ends and ligated with adapters for further PCR amplifications. The amplified cDNAs were sheared into 200 bp in length to make the sequencing library. The processed samples each in 10 ng were run in parallel at the platform Hiseq2000 of Beijing Genomics Institute (BGI). Paired-end reads of 75-nt were collected up to 1.2 giga bases (Gb) for each sample. Low quality reads were excluded from the raw data, and clean data sets (>1 Gb) were subject to the assembly pipeline described below.
Transcriptome reconstruction and annotation
The cleaned data of each sample was assembled using Trinity of the latest version (trinityrnaseq_r2012-06-08.tgz). The command line followed: Trinity.pl --seqType fa --JM 40G --left A.Left --right A.Right --CPU 8. Details on the parameters may be found in the associated manual (http://trinityrnaseq.sourceforge.net/#sample_data). The resulting assemblies were subsequently labeled as A, B, C, D, E, and F (Table 1), corresponding to the samples of Figure 1. The six samples were further combined to produce a final assembly using TGICL (TGICL-2.0.tar.gz) under the parameters: -p 95 -l 50 -v 6 -O '-h 3 -k 0 -o 50 -p 95'.
We annotated the final scaffolds of the combined data to a reference database containing a total of 2961141 uniref50 sequences (ftp.ebi.ac.uk/pub/databases/uniprot/, January 2012) by blastx under the parameters of -e 1e-5 –b 10. The resulted annotations were then re-organized by our local Perl scripts (Additional file 7) to extract information according to their GO (http://www.geneontology.org) and KEGG classifications (http://www.genome.jp/kegg/). The assembly of each sample was mapped back to the final assembly via Bowtie  to obtain the reads distribution among the entries. When the read number for an entry was less than two, we considered the result fortuitous, and took the expression as zero.
Normalization of multiple samples
We listed the maximum Mg (M max), the minimum Mg (M min), the maximum Ag (A max), and the minimum Ag (A min). If , , and then g was kept. These chosen genes formed G* (in our calculations: Mlower = 0.3, Mupper = 1 - 0.3; Alower = 0.2, Aupper = 1 - 0.2). From G* and the abundance levels of gene g in each sample (including the reference sample), we obtained the normalization factor R of each sample relative to the reference sample using the equation (1) above.
Chloroplast & mitochondrial mapping
The mapping of scaffolds of each sample to the chloroplast genome was performed by querying the known genome of the common morning glory using blastn under 1e-5 and parameters of identity ≥95%, and length ≥ 80%. For the mitochondrial genome, a similar query was made under 1e-5 and identity ≥ 80%, and length ≥ 50% against Nicotiana mitochondrial genome. The mapping results were then examined to exclude misplacements based on the blast results. We developed a series of scripts to partition and organize data files to classify subcellular genomic expressions, and to sort out the transcript distribution among samples (Additional file 8), which allowed later charting of data via Excel.
Verifications by sequencing and real-time qPCR
Petal cDNAs were obtained as previously mentioned. Primers were designed for the coding regions of target genes (Additional file 9; Additional file 3 of ) based on the final NGS data assembly, and applied to RT-PCR reactions using a standard protocol (Life Technologies). The amplified fragments were cloned and taken as the templates in sequencing reactions with big dye (Life Technologies). Samples for real-time qPCRs were similarly prepared and followed a previous protocol . The results were expressed as numbers of transcript copies per pg cDNA (Additional file 9).
Mapping sample patterns on molecular networks
All scaffolds were screened for connections to the known pathways in the KEGG database (Additional file 6). For each KEGG pathway, expressions of its components were first labeled to indicate the presences in the final assembly. When a specific pathway was in focus, the known topology was scrutinized at each component for its expression pattern among samples. Besides annotation from uniprot, the identity of a scaffold involved in the pathway was further verified via blast searches to NCBI databases (http://www.ncbi.nih.gov/) and its function cited by the enzyme nomenclature (http://www.chem.qmul.ac.uk/) and literatures. The close examinations allowed establishments of species-specific pathways with all of the components supported by the transcriptomic data. Organ-specific expressions were based on normalized scaffold levels and presented by labeling each component with a sign(s) representative of different expression patterns among organs. When a scaffold abundance level varied more than 2-fold between samples, we tentatively marked it as changing significantly. Assuming that a transcript level was reflective of the protein level to a significant degree, we were able to detect the direction of metabolic flux using criteria of the catalytic features of the enzymes and the expression patterns between the target transcriptomes (A, C, D, E) and reference ones (B, F) marked on the pathways.
Pearson correlation coefficients were computed for gene expression data, and a standard t-test was applied to the correlation coefficients. A non-parametric Wilcoxon rank test was conducted for comparing the mean of a sample in a small size to a specific value. Pearson correlation coefficients were also calculated between expression levels of scaffolds across samples, or between samples across scaffolds, which allowed clustering analysis via the agglomerative method implemented in Cluto (http://glaros.dtc.umn.edu/gkhome/fetch/sw/cluto/cluto-2.1.1.tar.gz) using maxtf scaling as our row model.
Availability of supporting data
All cleaned short reads have been submitted to GenBank (http://www.ncbi.nih.gov/) under the SRA accession number SRP015900. The final assembly of the sequences has also been submitted under the TSA accession number GALY01000000. Other supporting data are available in the additional files online.
We thank the reviewers for their thoughtful comments, H.-L. Zhou and L.-L. Xie for their assistances at various phases of this project and the group members of the ecological and evolutionary genomics (EEG) at the Institute of Botany, Chinese Academy of Sciences, for maintaining field plants and providing comments on this study. This work was supported by the Chinese Academy of Sciences (KSCX2-YW-N-043), the National Science Foundation of China (31070263, 30121003), and the Ministry of Science and Technology.
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