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  1. The concept of metabolite profiling has been around for decades and technical innovations are now enabling it to be carried out on a large scale with respect to the number of both metabolites measured and expe...

    Authors: Anna Lytovchenko, Romina Beleggia, Nicolas Schauer, Tal Isaacson, Jan E Leuendorf, Hanjo Hellmann, Jocelyn KC Rose and Alisdair R Fernie
    Citation: Plant Methods 2009 5:4
  2. Generating and identifying transformants is essential for many studies of gene function. In Arabidopsis thaliana, a revolutionary protocol termed floral dip is now the most widely used transformation method. Alth...

    Authors: Amanda M Davis, Anthony Hall, Andrew J Millar, Chiarina Darrah and Seth J Davis
    Citation: Plant Methods 2009 5:3
  3. A plethora of concurrent cellular activities is mobilised in the adaptation of plants to adverse environmental conditions. This response can be quantified by physiological experiments or metabolic profiling. T...

    Authors: Livia Saleh and Christoph Plieth
    Citation: Plant Methods 2009 5:2
  4. The investigation of protein-protein interactions is important for characterizing protein function. Bimolecular fluorescence complementation (BiFC) has recently gained interest as a relatively easy and inexpen...

    Authors: Lan-Ying Lee, Mei-Jane Fang, Lin-Yun Kuang and Stanton B Gelvin
    Citation: Plant Methods 2008 4:24
  5. Plant transformation is an invaluable tool for basic plant research, as well as a useful technique for the direct improvement of commercial crops. Barley (Hordeum vulgare) is the fourth most abundant cereal crop ...

    Authors: Joanne G Bartlett, Sílvia C Alves, Mark Smedley, John W Snape and Wendy A Harwood
    Citation: Plant Methods 2008 4:22
  6. The actin cytoskeleton responds quickly to diverse stimuli and plays numerous roles in cellular signalling, organelle motility and subcellular compartmentation during plant growth and development. Molecular an...

    Authors: Mike Schenkel, Alison M Sinclair, Daniel Johnstone, JDerek Bewley and Jaideep Mathur
    Citation: Plant Methods 2008 4:21
  7. Allotetraploid white clover (Trifolium repens L.) is an important forage legume widely cultivated in most temperate regions. Only a small number of microsatellite markers are publicly available and can be utilize...

    Authors: Yan Zhang, Ji He, Patrick X Zhao, Joseph H Bouton and Maria J Monteros
    Citation: Plant Methods 2008 4:19
  8. Medicago truncatula is a model legume species that is currently the focus of an international genome sequencing effort. Although several different oligonucleotide and cDNA arrays have been produced for genome-wid...

    Authors: Klementina Kakar, Maren Wandrey, Tomasz Czechowski, Tanja Gaertner, Wolf-Rüdiger Scheible, Mark Stitt, Ivone Torres-Jerez, Yongli Xiao, Julia C Redman, Hank C Wu, Foo Cheung, Christopher D Town and Michael K Udvardi
    Citation: Plant Methods 2008 4:18
  9. Transcription factors (TFs) co-ordinately regulate target genes that are dispersed throughout the genome. This co-ordinate regulation is achieved, in part, through the interaction of transcription factors with...

    Authors: Andrew P Dare, Robert J Schaffer, Kui Lin-Wang, Andrew C Allan and Roger P Hellens
    Citation: Plant Methods 2008 4:17
  10. We describe an efficient method for the rapid quantitative determination of the abundance of three acidic plant hormones from a single crude extract directly by LC/MS/MS. The method exploits the sensitivity of...

    Authors: Silvia Forcat, Mark H Bennett, John W Mansfield and Murray R Grant
    Citation: Plant Methods 2008 4:16
  11. Quantitative Real Time RT-PCR (q2(RT)PCR) is a maturing technique which gives researchers the ability to quantify and compare very small amounts of nucleic acids. Primer design and optimization is an essential...

    Authors: Richard M Sharpe, Sade N Dunn and A Bruce Cahoon
    Citation: Plant Methods 2008 4:14
  12. We report the development of a microarray platform for rapid and cost-effective genetic mapping, and its evaluation using rice as a model. In contrast to methods employing whole-genome tiling microarrays for g...

    Authors: Jeremy D Edwards, Jaroslav Janda, Megan T Sweeney, Ambika B Gaikwad, Bin Liu, Hei Leung and David W Galbraith
    Citation: Plant Methods 2008 4:13
  13. For omics experiments, detailed characterisation of experimental material with respect to its genetic features, its cultivation history and its treatment history is a requirement for analyses by bioinformatics...

    Authors: Karin I Köhl, Georg Basler, Alexander Lüdemann, Joachim Selbig and Dirk Walther
    Citation: Plant Methods 2008 4:11
  14. The genus Brassica (Brassicaceae, Brassiceae) is closely related to the model plant Arabidopsis, and includes several important crop plants. Against the background of ongoing genome sequencing, and in line with e...

    Authors: Lars Østergaard and Graham J King
    Citation: Plant Methods 2008 4:10
  15. The isolation of green fluorescent protein (GFP) and the development of spectral variants over the past decade have begun to reveal the dynamic nature of protein trafficking and organelle motility. In planta anal...

    Authors: Naohiro Kato, Dexter Reynolds, Matthew L Brown, Marietta Boisdore, Yukichi Fujikawa, Andrea Morales and Lee A Meisel
    Citation: Plant Methods 2008 4:9
  16. Microsatellites are popular molecular markers in many plant species due to their stable and highly polymorphic nature. A number of analysis methods have been described but analyses of these markers are typical...

    Authors: John F Mackay, Christopher D Wright and Roderick G Bonfiglioli
    Citation: Plant Methods 2008 4:8
  17. A common limitation in guard cell signaling research is that it is difficult to obtain consistent high expression of transgenes of interest in Arabidopsis guard cells using known guard cell promoters or the const...

    Authors: Yingzhen Yang, Alex Costa, Nathalie Leonhardt, Robert S Siegel and Julian I Schroeder
    Citation: Plant Methods 2008 4:6
  18. Protein S-acylation (also known as palmitoylation) is the reversible post-translational addition of acyl lipids to cysteine residues in proteins through a thioester bond. It allows strong association with memb...

    Authors: Piers A Hemsley, Laura Taylor and Claire S Grierson
    Citation: Plant Methods 2008 4:2
  19. Research in plant science laboratories often involves usage of many different species, cultivars, ecotypes, mutants, alleles or transgenic lines. This creates a great challenge to keep track of the identity of...

    Authors: Vivien Exner, Matthias Hirsch-Hoffmann, Wilhelm Gruissem and Lars Hennig
    Citation: Plant Methods 2008 4:1
  20. MicroRNAs (miRNAs) are a class of small non-coding RNAs with a critical role in development and environmental responses. Efficient and reliable detection of miRNAs is an essential step towards understanding th...

    Authors: Erika Varkonyi-Gasic, Rongmei Wu, Marion Wood, Eric F Walton and Roger P Hellens
    Citation: Plant Methods 2007 3:12
  21. Chromatin remodeling, histone modifications and other chromatin-related processes play a crucial role in gene regulation. A very useful technique to study these processes is chromatin immunoprecipitation (ChIP...

    Authors: Max Haring, Sascha Offermann, Tanja Danker, Ina Horst, Christoph Peterhansel and Maike Stam
    Citation: Plant Methods 2007 3:11
  22. The results of transcriptome microarray analysis are usually presented as a list of differentially expressed genes. As these lists can be long, it is hard to interpret the desired experimental treatment effect...

    Authors: Ana Rotter, Björn Usadel, Špela Baebler, Mark Stitt and Kristina Gruden
    Citation: Plant Methods 2007 3:10
  23. Quantitative reverse transcription – polymerase chain reaction (qRT-PCR) has been demonstrated to be particularly suitable for the analysis of weakly expressed genes, such as those encoding transcription facto...

    Authors: Camila Caldana, Wolf-Rüdiger Scheible, Bernd Mueller-Roeber and Slobodan Ruzicic
    Citation: Plant Methods 2007 3:7
  24. The diploid, Solanum caripense, a wild relative of potato and tomato, possesses valuable resistance to potato late blight and we are interested in the genetic base of this resistance. Due to extremely low levels ...

    Authors: Joy Nakitandwe, Friederike Trognitz and Bodo Trognitz
    Citation: Plant Methods 2007 3:2
  25. There is considerable interest in rapid assays or screening systems for assigning gene function. However, analysis of gene function in the flowers of some species is restricted due to the difficulty of produci...

    Authors: Yongjin Shang, Kathy E Schwinn, Michael J Bennett, Donald A Hunter, Toni L Waugh, Nilangani N Pathirana, David A Brummell, Paula E Jameson and Kevin M Davies
    Citation: Plant Methods 2007 3:1
  26. Genome analysis of soybean (Glycine max L.) has been complicated by its paleo-autopolyploid nature and conserved homeologous regions. Landmarks of expressed sequence tags (ESTs) located within a minimum tile path...

    Authors: Kay L Shopinski, Muhammad J Iqbal, Jeffry L Shultz, Dheepakkumaran Jayaraman and David A Lightfoot
    Citation: Plant Methods 2006 2:20
  27. The floral dip method of transformation by immersion of inflorescences in a suspension of Agrobacterium is the method of choice for Arabidopsis transformation. The presence of a marker, usually antibiotic- or her...

    Authors: Samuel J Harrison, Ellie K Mott, Kate Parsley, Sue Aspinall, John C Gray and Amanda Cottage
    Citation: Plant Methods 2006 2:19
  28. Genetic engineering of commercially important crops has become routine in many laboratories. However, the inability to predict where a transgene will integrate and to efficiently select plants with stable leve...

    Authors: Ana Paula Santos, Eva Wegel, George C Allen, William F Thompson, Eva Stoger, Peter Shaw and Rita Abranches
    Citation: Plant Methods 2006 2:18
  29. We have developed a functional genomics approach based on expression cloning in Xenopus oocytes to identify plant transporter function. We utilized the full-length cDNA databases to generate a normalized library ...

    Authors: Hussam H Nour-Eldin, Morten HH Nørholm and Barbara A Halkier
    Citation: Plant Methods 2006 2:17

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