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  1. The Agrobacterium vacuum (Bechtold et al 1993) and floral-dip (Clough and Bent 1998) are very efficient methods for generating transgenic Arabidopsis plants. These methods allow plant transformation without the n...

    Authors: Elke Logemann, Rainer P Birkenbihl, Bekir Ülker and Imre E Somssich
    Citation: Plant Methods 2006 2:16
  2. Mg chelatase is a multi-subunit enzyme that catalyses the first committed step of chlorophyll biosynthesis. Studies in higher plants and algae indicate that the Mg chelatase reaction product, Mg-protoporphyrin...

    Authors: Ruairidh JH Sawers, Phyllis R Farmer, Peter Moffett and Thomas P Brutnell
    Citation: Plant Methods 2006 2:15
  3. Strategies for robust quantitative comparison between different biological samples are of high importance in experiments that address biological questions beyond the establishment of protein lists. Here, we pr...

    Authors: Wolfgang R Engelsberger, Alexander Erban, Joachim Kopka and Waltraud X Schulze
    Citation: Plant Methods 2006 2:14
  4. Transient assays using protoplasts are ideal for processing large quantities of genetic data coming out of hi-throughput assays. Previously, protoplasts have routinely been prepared from dicot tissue or cell s...

    Authors: Rebecca Bart, Mawsheng Chern, Chang-Jin Park, Laura Bartley and Pamela C Ronald
    Citation: Plant Methods 2006 2:13
  5. The ultimate goal of proteomic analysis of a cell compartment should be the exhaustive identification of resident proteins; excluding proteins from other cell compartments. Reaching such a goal closely depends...

    Authors: Leila Feiz, Muhammad Irshad, Rafael F Pont-Lezica, Hervé Canut and Elisabeth Jamet
    Citation: Plant Methods 2006 2:10
  6. The creation of minimally redundant tile paths (hereafter MTP) from contiguous sets of overlapping clones (hereafter contigs) in physical maps is a critical step for structural and functional genomics. Build 4...

    Authors: JL Shultz, C Yesudas, S Yaegashi, AJ Afzal, S Kazi and DA Lightfoot
    Citation: Plant Methods 2006 2:9
  7. Uncovering the key sequence elements in gene promoters that regulate the expression of plant genomes is a huge task that will require a series of complementary methods for prediction, substantial innovations i...

    Authors: Kathryn E Holt, A Harvey Millar and James Whelan
    Citation: Plant Methods 2006 2:8
  8. The pH is an important parameter controlling many metabolic and signalling pathways in living cells. Recombinant fluorescent pH indicators (pHluorins) have come into vogue for monitoring cellular pH. They are ...

    Authors: Alexander Schulte, Inken Lorenzen, Markus Böttcher and Christoph Plieth
    Citation: Plant Methods 2006 2:7
  9. As an Open Access journal dedicated to promoting technological innovation in plant biology, Plant Methods occupies a unique niche amongst plant journals. To fund its open access policy, and to enable it to contin...

    Authors: Brian G Forde and Michael R Roberts
    Citation: Plant Methods 2006 2:6
  10. In order to understand microarray data reasonably in the context of other existing biological knowledge, it is necessary to conduct a thorough examination of the data utilizing every aspect of available omic k...

    Authors: Yoshikazu Hasegawa, Motoaki Seki, Yoshiki Mochizuki, Naohiko Heida, Katsura Hirosawa, Naoki Okamoto, Tetsuya Sakurai, Masakazu Satou, Kenji Akiyama, Kei Iida, Kisik Lee, Shigehiko Kanaya, Taku Demura, Kazuo Shinozaki, Akihiko Konagaya and Tetsuro Toyoda
    Citation: Plant Methods 2006 2:5
  11. The preparation of expressional cDNA libraries for use in the yeast two-hybrid system is quick and efficient when using the dedicated Clontech™ product, the MATCHMAKER Library Construction and Screening Kit 3....

    Authors: Sergiy Lopato, Natalia Bazanova, Sarah Morran, Andrew S Milligan, Neil Shirley and Peter Langridge
    Citation: Plant Methods 2006 2:3
  12. Abscission is the regulated dropping of plant organs, such as leaves or flower petals. This process involves a break down of the cell wall between layers of cells in the abscission zone, causing the organ to b...

    Authors: Kevin A Lease, Sung Ki Cho and John C Walker
    Citation: Plant Methods 2006 2:2
  13. Appropriate biological interpretation of microarray data calls for relevant experimental annotation. The widely accepted MIAME guidelines provide a generic, organism-independant standard for minimal informatio...

    Authors: Philip Zimmermann, Beatrice Schildknecht, David Craigon, Margarita Garcia-Hernandez, Wilhelm Gruissem, Sean May, Gaurab Mukherjee, Helen Parkinson, Seung Rhee, Ulrich Wagner and Lars Hennig
    Citation: Plant Methods 2006 2:1
  14. In silico analyses based on sequence similarities with animal channels have identified a large number of plant genes likely to encode ion channels. The attempts made to characterise such putative plant channel...

    Authors: E Hosy, G Duby, A-A Véry, A Costa, H Sentenac and J-B Thibaud
    Citation: Plant Methods 2005 1:14
  15. We describe novel plasmid vectors for transient gene expression using Agrobacterium, infiltrated into Nicotiana benthamiana leaves. We have generated a series of pGreenII cloning vectors that are ideally suited t...

    Authors: Roger P Hellens, Andrew C Allan, Ellen N Friel, Karen Bolitho, Karryn Grafton, Matthew D Templeton, Sakuntala Karunairetnam, Andrew P Gleave and William A Laing
    Citation: Plant Methods 2005 1:13
  16. High-density oligonucleotide (oligo) arrays are a powerful tool for transcript profiling. Arrays based on GeneChip® technology are amongst the most widely used, although GeneChip® arrays are currently available f...

    Authors: John P Hammond, Martin R Broadley, David J Craigon, Janet Higgins, Zoe F Emmerson, Henrik J Townsend, Philip J White and Sean T May
    Citation: Plant Methods 2005 1:10
  17. Protein phosphorylation is accepted as a major regulatory pathway in plants. More than 1000 protein kinases are predicted in the Arabidopsis proteome, however, only a few studies look systematically for in vivo p...

    Authors: Florian Wolschin and Wolfram Weckwerth
    Citation: Plant Methods 2005 1:9
  18. In situ hybridisation can provide cellular, and in some cases sub-cellular, resolution of mRNA levels within multicellular organisms and is widely used to provide spatial and temporal information on gene expressi...

    Authors: Sinéad Drea, Julia Corsar, Brian Crawford, Peter Shaw, Liam Dolan and John H Doonan
    Citation: Plant Methods 2005 1:8
  19. Eukaryotic organisms are defined by the presence of a nucleus, which encloses the chromosomal DNA, and is characterized by its DNA content (C-value). Complex eukaryotic organisms contain organs and tissues tha...

    Authors: Changqing Zhang, Fang Cheng Gong, Georgina M Lambert and David W Galbraith
    Citation: Plant Methods 2005 1:7
  20. Plant genome sequence data now provide opportunities to conduct molecular genetic studies at the level of the whole gene locus and above. Such studies will be greatly facilitated by adopting and developing fur...

    Authors: Laura C Roden, Berthold Göttgens and Effie S Mutasa-Göttgens
    Citation: Plant Methods 2005 1:6
  21. Many established PCR-based approaches in plant molecular biology rely on lengthy and expensive methods for isolation of nucleic acids. Although several rapid DNA isolation protocols are available, they have no...

    Authors: Kenneth Berendzen, Iain Searle, Dean Ravenscroft, Csaba Koncz, Alfred Batschauer, George Coupland, Imre E Somssich and Bekir Ülker
    Citation: Plant Methods 2005 1:4

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