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  1. Recent advances in genome sequencing technologies have shifted the research bottleneck in plant sciences from genotyping to phenotyping. This shift has driven the development of phenomics, high-throughput non-...

    Authors: Pádraic J. Flood, Willem Kruijer, Sabine K. Schnabel, Rob van der Schoor, Henk Jalink, Jan F. H. Snel, Jeremy Harbinson and Mark G. M. Aarts
    Citation: Plant Methods 2016 12:14
  2. Virus-induced gene silencing (VIGS) has become an emerging technology for the rapid, efficient functional genomic screening of monocot and dicot species. The barley stripe mosaic virus (BSMV) has been describ...

    Authors: Leann M. Buhrow, Shawn M. Clark and Michele C. Loewen
    Citation: Plant Methods 2016 12:12
  3. The efficiency, versatility and multiplexing capacity of RNA-guided genome engineering using the CRISPR/Cas9 technology enables a variety of applications in plants, ranging from gene editing to the constructi...

    Authors: Marta Vazquez-Vilar, Joan Miquel Bernabé-Orts, Asun Fernandez-del-Carmen, Pello Ziarsolo, Jose Blanca, Antonio Granell and Diego Orzaez
    Citation: Plant Methods 2016 12:10
  4. Plant growth is a good indicator of crop performance and can be measured by different methods and on different spatial and temporal scales. In this study, we measured the canopy height growth of maize (Zea mays),...

    Authors: Michael Friedli, Norbert Kirchgessner, Christoph Grieder, Frank Liebisch, Michael Mannale and Achim Walter
    Citation: Plant Methods 2016 12:9
  5. Targeted genome engineering has been described as a “game-changing technology” for fields as diverse as human genetics and plant biotechnology. One technique used for precise gene editing utilises the CRISPR-C...

    Authors: Geraint Parry, Nicola Patron, Ruth Bastow and Colette Matthewman
    Citation: Plant Methods 2016 12:6
  6. A screening method for elicitor and priming agents does not only allow detecting new bioactive substances, it can also be used to understand structure–function relationships of known agents by testing differen...

    Authors: Rebecca L. J. Melcher and Bruno M. Moerschbacher
    Citation: Plant Methods 2016 12:5
  7. Establishment and maintenance of mutualistic plant–microbial interactions in the rhizosphere and within plant roots involve several root cell types. The processes of host–microbe recognition and infection requ...

    Authors: Srdjan Gavrilovic, Zhe Yan, Anna M. Jurkiewicz, Jens Stougaard and Katharina Markmann
    Citation: Plant Methods 2016 12:4
  8. The deposition of silicon into epidermal cells of grass species is thought to be an important mechanism that plants use as a defense against pests and environmental stresses. There are a number of techniques a...

    Authors: Clemon Dabney III, Jason Ostergaard, Eric Watkins and Changbin Chen
    Citation: Plant Methods 2016 12:3
  9. There has been an exponential growth in the number of genome sequencing projects since the introduction of next generation DNA sequencing technologies. Genome projects have increasingly involved assembly of wh...

    Authors: Paul Visendi, Paul J. Berkman, Satomi Hayashi, Agnieszka A. Golicz, Philipp E. Bayer, Pradeep Ruperao, Bhavna Hurgobin, Juan Montenegro, Chon-Kit Kenneth Chan, Helena Staňková, Jacqueline Batley, Hana Šimková, Jaroslav Doležel and David Edwards
    Citation: Plant Methods 2016 12:2
  10. Piercing-sucking insects cause severe damage in crops. Breeding for host-plant resistance can significantly reduce the yield losses caused by these insects, but host-plant resistance is a complex trait that is...

    Authors: Manus P. M. Thoen, Karen J. Kloth, Gerrie L. Wiegers, Olga E. Krips, Lucas P. J. J. Noldus, Marcel Dicke and Maarten A. Jongsma
    Citation: Plant Methods 2016 12:1
  11. Pseudomonas syringae can cause stem necrosis and canker in a wide range of woody species including cherry, plum, peach, horse chestnut and ash. The detection and quantification of lesi...

    Authors: Bo Li, Michelle T. Hulin, Philip Brain, John W. Mansfield, Robert W. Jackson and Richard J. Harrison
    Citation: Plant Methods 2015 11:57
  12. Despite increasing demand, imaging the internal structure of plant organs or tissues without the use of transgenic lines expressing fluorescent proteins remains a challenge. Techniques such as magnetic resonan...

    Authors: David Rousseau, Thomas Widiez, Sylvaine Di Tommaso, Hugo Rositi, Jerome Adrien, Eric Maire, Max Langer, Cécile Olivier, Françoise Peyrin and Peter Rogowsky
    Citation: Plant Methods 2015 11:55
  13. To our knowledge, there is no software or database solution that supports large volumes of biological time series sensor data efficiently and enables data visualization and analysis in real time. Existing solu...

    Authors: Ali Salehi, Jose Jimenez-Berni, David M. Deery, Doug Palmer, Edward Holland, Pablo Rozas-Larraondo, Scott C. Chapman, Dimitrios Georgakopoulos and Robert T. Furbank
    Citation: Plant Methods 2015 11:53
  14. The flag leaf of a wheat (Triticum aestivum L.) plant rolls up into a cylinder in response to drought conditions and then unrolls when leaf water relations improve. This is a desirable trait for extending leaf a...

    Authors: X. R. R. Sirault, A. G. Condon, J. T. Wood, G. D. Farquhar and G. J. Rebetzke
    Citation: Plant Methods 2015 11:52
  15. Plant root systems are key drivers of plant function and yield. They are also under-explored targets to meet global food and energy demands. Many new technologies have been developed to characterize crop root ...

    Authors: Abhiram Das, Hannah Schneider, James Burridge, Ana Karine Martinez Ascanio, Tobias Wojciechowski, Christopher N. Topp, Jonathan P. Lynch, Joshua S. Weitz and Alexander Bucksch
    Citation: Plant Methods 2015 11:51
  16. Rapid advances in microscopy have boosted research on cell biology. However sample preparation enabling excellent reproducible tissue preservation and cell labeling for in depth microscopic analysis of inner c...

    Authors: Taras Pasternak, Olaf Tietz, Katja Rapp, Maura Begheldo, Roland Nitschke, Benedetto Ruperti and Klaus Palme
    Citation: Plant Methods 2015 11:50
  17. Since the quality and yield of rice production depends on endosperm development, previous studies have focused on the molecular mechanism that regulates this developmental process. Recently, how this process i...

    Authors: Quan Kuang, Xiaobo Yu, Xiongbo Peng and Meng-xiang Sun
    Citation: Plant Methods 2015 11:49
  18. Photosynthetic assimilation of carbon is a defining feature of the plant kingdom. The fixation of large amounts of carbon dioxide supports the synthesis of carbohydrates, which make up the bulk of plant biomas...

    Authors: Katharina Kölling, Gavin M. George, Roland Künzli, Patrick Flütsch and Samuel C. Zeeman
    Citation: Plant Methods 2015 11:48
  19. Plant growth chambers provide a controlled environment to analyse the effects of environmental parameters (light, temperature, atmospheric gas composition etc.) on plant function. However, it has been shown th...

    Authors: Amanda S. Porter, Christiana Evans-Fitz.Gerald, Jennifer C. McElwain, Charilaos Yiotis and Caroline Elliott-Kingston
    Citation: Plant Methods 2015 11:44
  20. The importance of appropriate, accurate measurement and reporting of environmental parameters in plant sciences is a significant aspect of quality assurance for all researchers and their research. There is a c...

    Authors: A J Both, L Benjamin, J Franklin, G Holroyd, L D Incoll, M G Lefsrud and G Pitkin
    Citation: Plant Methods 2015 11:43
  21. Identifying cis-regulatory elements is critical in understanding the direct and indirect regulatory mechanisms of gene expression. Current approaches include DNase-seq, a technique that combines sensitivity to th...

    Authors: Jason S. Cumbie, Sergei A. Filichkin and Molly Megraw
    Citation: Plant Methods 2015 11:42
  22. X-ray computed tomography (CT) has become a powerful tool for root phenotyping. Compared to rather classical, destructive methods, CT encompasses various advantages. In pot experiments the growth and developme...

    Authors: Johannes Pfeifer, Norbert Kirchgessner, Tino Colombi and Achim Walter
    Citation: Plant Methods 2015 11:41
  23. Bread wheat (Triticum aestivum L., 2n = 6x = 42) is an allohexaploid with a huge genome. Due to the presence of extensive homoeologs and paralogs, generating locus-specific sequences can be challenging, especiall...

    Authors: Jian Ma, Jiri Stiller, Zhi Zheng, Ya-Xi Liu, Yuming Wei, You-Liang Zheng and Chunji Liu
    Citation: Plant Methods 2015 11:39
  24. Higher plants have evolved sophisticated communication systems to integrate environmental stimuli into their developmental programs. Grafting provides a powerful technique to examine transportation and systemi...

    Authors: Nien-Chen Huang and Tien-Shin Yu
    Citation: Plant Methods 2015 11:38
  25. Recent developments in unmanned aerial platforms (UAP) have provided research opportunities in assessing land allocation and crop physiological traits, including response to abiotic and biotic stresses. UAP-ba...

    Authors: M Zaman-Allah, O Vergara, J L Araus, A Tarekegne, C Magorokosho, P J Zarco-Tejada, A Hornero, A Hernández Albà, B Das, P Craufurd, M Olsen, B M Prasanna and J Cairns
    Citation: Plant Methods 2015 11:35
  26. The genus Cuscuta is a group of parasitic plants that are distributed world-wide. The process of parasitization starts with a Cuscuta plant coiling around the host stem. The parasite’s haustorial organs then esta...

    Authors: Daisuke Ikeue, Christian Schudoma, Wenna Zhang, Yoshiyuki Ogata, Tomoaki Sakamoto, Tetsuya Kurata, Takeshi Furuhashi, Friedrich Kragler and Koh Aoki
    Citation: Plant Methods 2015 11:34
  27. A well characterized output of the circadian clock in plants is the daily rhythmic movement of leaves. This process has been used extensively in Arabidopsis to estimate circadian period in natural accessions a...

    Authors: Kathleen Greenham, Ping Lou, Sara E Remsen, Hany Farid and C Robertson McClung
    Citation: Plant Methods 2015 11:33
  28. Over the last two decades, the development of high-throughput techniques has enabled us to probe the plant circadian clock, a key coordinator of vital biological processes, in ways previously impossible. With ...

    Authors: Andrew J Tindall, Jade Waller, Mark Greenwood, Peter D Gould, James Hartwell and Anthony Hall
    Citation: Plant Methods 2015 11:32
  29. The use of light emitting diodes (LEDs) brings several key advantages over existing illumination technologies for indoor plant cultivation. Among these are that LEDs have predicted lifetimes from 50–100.000 ho...

    Authors: Martin Janda, Oldřich Navrátil, Daniel Haisel, Barbora Jindřichová, Jan Fousek, Lenka Burketová, Noemi Čeřovská and Tomáš Moravec
    Citation: Plant Methods 2015 11:31
  30. Genetic markers are tools that can facilitate molecular breeding, even in species lacking genomic resources. An important class of genetic markers is those based on orthologous genes, because they can guide hy...

    Authors: Jin-Hyun Kim, Chaeyoung Lee, Daejin Hyung, Ye-Jin Jo, Joo-Seok Park, Douglas R Cook and Hong-Kyu Choi
    Citation: Plant Methods 2015 11:30
  31. Current methods of in-house plant phenotyping are providing a powerful new tool for plant biology studies. The self-constructed and commercial platforms established in the last few years, employ non-destructiv...

    Authors: Jan F Humplík, Dušan Lazár, Alexandra Husičková and Lukáš Spíchal
    Citation: Plant Methods 2015 11:29
  32. The detection and characterization of resistance reactions of crop plants against fungal pathogens are essential to select resistant genotypes. In breeding practice phenotyping of plant genotypes is realized b...

    Authors: Matheus Kuska, Mirwaes Wahabzada, Marlene Leucker, Heinz-Wilhelm Dehne, Kristian Kersting, Erich-Christian Oerke, Ulrike Steiner and Anne-Katrin Mahlein
    Citation: Plant Methods 2015 11:28
  33. Grass stalks architecturally support leaves and reproductive structures, functionally support the transport of water and nutrients, and are harvested for multiple agricultural uses. Research on these basic and...

    Authors: Sven Heckwolf, Marlies Heckwolf, Shawn M Kaeppler, Natalia de Leon and Edgar P Spalding
    Citation: Plant Methods 2015 11:26
  34. Image analysis is increasingly used in plant phenotyping. Among the various imaging techniques that can be used in plant phenotyping, chlorophyll fluorescence imaging allows imaging of the impact of biotic or ...

    Authors: Céline Rousseau, Gilles Hunault, Sylvain Gaillard, Julie Bourbeillon, Gregory Montiel, Philippe Simier, Claire Campion, Marie-Agnès Jacques, Etienne Belin and Tristan Boureau
    Citation: Plant Methods 2015 11:24
  35. Effects of abiotic and biotic stresses on plant photosynthetic performance lead to fitness and yield decrease. The maximum quantum efficiency of photosystem II (F v/F m) is a p...

    Authors: Justine Bresson, François Vasseur, Myriam Dauzat, Garance Koch, Christine Granier and Denis Vile
    Citation: Plant Methods 2015 11:23

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