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  1. Pea has lagged behind other model legumes in the molecular study of nodulation and mycorrhizae-formation because of the difficulty to transform its roots and its poor growth on agar plates. Here we describe fo...

    Authors: Scott R Clemow, Lindsey Clairmont, Lene H Madsen and Frédérique C Guinel
    Citation: Plant Methods 2011 7:46
    Content type: Methodology Published on:

  2. In Arabidopsis thaliana we demonstrate that dying root hairs provide an easy and rapid in vivo model for the morphological identification of apoptotic-like programmed cell death (AL-PCD) in plants. The model desc...

    Authors: Bridget V Hogg, Joanna Kacprzyk, Elizabeth M Molony, Conor O'Reilly, Thomas F Gallagher, Patrick Gallois and Paul F McCabe
    Citation: Plant Methods 2011 7:45
    Content type: Methodology Published on:

  4. Targeted Induced Loci Lesions IN Genomes (TILLING) is increasingly being used to generate and identify mutations in target genes of crop genomes. TILLING populations of several thousand lines have been generat...

    Authors: Seosamh Ó Lochlainn, Stephen Amoah, Neil S Graham, Khalid Alamer, Juan J Rios, Smita Kurup, Andrew Stoute, John P Hammond, Lars Østergaard, Graham J King, Phillip J White and Martin R Broadley
    Citation: Plant Methods 2011 7:43
    Content type: Methodology Published on:

  6. To simulate expected future global warming, hexagonal arrays of infrared heaters have previously been used to warm open-field canopies of upland crops such as wheat. Through the use of concrete-anchored posts,...

    Authors: Muhammad Ishaq Asif Rehmani, Jingqi Zhang, Ganghua Li, Syed Tahir Ata-Ul-Karim, Shaohua Wang, Bruce A Kimball, Chuan Yan, Zhenghui Liu and Yanfeng Ding
    Citation: Plant Methods 2011 7:41
    Content type: Methodology Published on:

  8. Accurate analysis of DNA methylation by bisulphite sequencing depends on the complete conversion of all cytosines into uracil. Until now there has been no standard or universal gene identified as an endogenous...

    Authors: Jing Wang, Chongnan Wang, Yan Long, Clare Hopkins, Smita Kurup, Kede Liu, Graham J King and Jinling Meng
    Citation: Plant Methods 2011 7:39
    Content type: Methodology Published on:

  9. Complete organellar genome sequences (chloroplasts and mitochondria) provide valuable resources and information for studying plant molecular ecology and evolution. As high-throughput sequencing technology adva...

    Authors: Tongwu Zhang, Xiaowei Zhang, Songnian Hu and Jun Yu
    Citation: Plant Methods 2011 7:38
    Content type: Methodology Published on:

  10. Plant hormones play a pivotal role in several physiological processes during a plant's life cycle, from germination to senescence, and the determination of endogenous concentrations of hormones is essential to...

    Authors: Maren Müller and Sergi Munné-Bosch
    Citation: Plant Methods 2011 7:37
    Content type: Methodology Published on:

  11. Transformation and subsequent regeneration of holoparasitic plants has never been reported, in part due to challenges in developing transformation protocols, but also because regeneration of obligate parasites...

    Authors: Mónica Fernández-Aparicio, Diego Rubiales, Pradeepa CG Bandaranayake, John I Yoder and James H Westwood
    Citation: Plant Methods 2011 7:36
    Content type: Methodology Published on:

  13. To adjust their development to the environment, plants rely on specific signals that travel from shoot to root and vice versa. Here we describe an efficient micrografting protocol for Nicotiana attenuata, a usefu...

    Authors: Variluska Fragoso, Hannah Goddard, Ian T Baldwin and Sang-Gyu Kim
    Citation: Plant Methods 2011 7:34
    Content type: Methodology Published on:

  14. Large-insert BAC and BIBAC libraries are important tools for structural and functional genomics studies of eukaryotic genomes. To facilitate the construction of BAC and BIBAC libraries and the transfer of comp...

    Authors: Xue Shi, Haiyang Zeng, Yadong Xue and Meizhong Luo
    Citation: Plant Methods 2011 7:33
    Content type: Methodology Published on:

  15. The Arabidopsis thaliana-Pseudomonas syringae model pathosystem is one of the most widely used systems to understand the mechanisms of microbial pathogenesis and plant innate immunity. Several inoculation methods...

    Authors: Yasuhiro Ishiga, Takako Ishiga, Srinivasa R Uppalapati and Kirankumar S Mysore
    Citation: Plant Methods 2011 7:32
    Content type: Methodology Published on:

  16. Microarray technology has matured over the past fifteen years into a cost-effective solution with established data analysis protocols for global gene expression profiling. The Agilent-016047 maize 44 K microar...

    Authors: Nanette Coetzer, Alexander A Myburg and Dave K Berger
    Citation: Plant Methods 2011 7:31
    Content type: Database Published on:

  17. Plant protoplasts, a proven physiological and versatile cell system, are widely used in high-throughput analysis and functional characterization of genes. Green protoplasts have been successfully used in inves...

    Authors: Yang Zhang, Jianbin Su, Shan Duan, Ying Ao, Jinran Dai, Jun Liu, Peng Wang, Yuge Li, Bing Liu, Dongru Feng, Jinfa Wang and Hongbin Wang
    Citation: Plant Methods 2011 7:30
    Content type: Methodology Published on:

  18. Plastids are small organelles equipped with their own genomes (plastomes). Although these organelles are involved in numerous plant metabolic pathways, current knowledge about the transcriptional activity of p...

    Authors: Agnieszka Żmieńko, Magdalena Guzowska-Nowowiejska, Radosław Urbaniak, Wojciech Pląder, Piotr Formanowicz and Marek Figlerowicz
    Citation: Plant Methods 2011 7:29
    Content type: Methodology Published on:

  19. Wheat is one of the most important crops in Australia, and the identification of young plants is an important step towards developing an automated system for monitoring crop establishment and also for differen...

    Authors: Mahmood R Golzarian and Ross A Frick
    Citation: Plant Methods 2011 7:28
    Content type: Methodology Published on:

  20. Total internal reflection fluorescence microscopy (TIRFM) is a powerful tool for observing fluorescently labeled molecules on the plasma membrane surface of animal cells. However, the utility of TIRFM in plant...

    Authors: Yinglang Wan, William M Ash III, Lusheng Fan, Huaiqin Hao, Myung K Kim and Jinxing Lin
    Citation: Plant Methods 2011 7:27
    Content type: Methodology Published on:

  21. We outline a high throughput procedure that improves outlier detection in cell wall screens using FT-NIR spectroscopy of plant leaves. The improvement relies on generating a calibration set from a subset of a ...

    Authors: Andreia M Smith-Moritz, Mawsheng Chern, Jeemeng Lao, Wing Hoi Sze-To, Joshua L Heazlewood, Pamela C Ronald and Miguel E Vega-Sánchez
    Citation: Plant Methods 2011 7:26
    Content type: Methodology Published on:

  22. Transient expression of proteins in plants has become a favoured method over the production of stably transformed plants because, in addition to enabling high protein yields, it is both fast and easy to apply....

    Authors: Dirk Stephan, Coba Slabber, Gavin George, Victor Ninov, Kevin P Francis and Johan T Burger
    Citation: Plant Methods 2011 7:25
    Content type: Methodology Published on:

  23. A method was developed to identify insertional mutants of Chlamydomonas reinhardtii disrupted for selected target genes. The approach relies on the generation of thousands of transformants followed by PCR-based s...

    Authors: David Gonzalez-Ballester, Wirulda Pootakham, Florence Mus, Wenqiang Yang, Claudia Catalanotti, Leonardo Magneschi, Amaury de Montaigu, Jose J Higuera, Matthew Prior, Aurora Galván, Emilio Fernandez and Arthur R Grossman
    Citation: Plant Methods 2011 7:24
    Content type: Methodology Published on:

  24. Prenylquinones are key compounds of the thylakoid membranes in chloroplasts. To understand the mechanisms involved in the response of plants to changing conditions such as high light intensity, the comprehensi...

    Authors: Jacopo Martinis, Felix Kessler and Gaetan Glauser
    Citation: Plant Methods 2011 7:23
    Content type: Methodology Published on:

  27. Cultivated sunflower (Helianthus annus L.) is a globally important oilseed crop, subjected to intensive genetic and genomic studies. Although classical mutagenesis has successfully been applied to Helianthus genu...

    Authors: Wilma Sabetta, Vittorio Alba, Antonio Blanco and Cinzia Montemurro
    Citation: Plant Methods 2011 7:20
    Content type: Research Published on:

  28. The biology of the seed is complicated by the extensive non-homogeneity (spatial gradients) in gene expression, metabolic conversions and storage product accumulation. The detailed understanding of the mechani...

    Authors: Silke Schiebold, Henning Tschiersch, Ljudmilla Borisjuk, Nicolas Heinzel, Ruslana Radchuk and Hardy Rolletschek
    Citation: Plant Methods 2011 7:19
    Content type: Methodology Published on:

  29. The tomato (Solanum lycopersicum L.) plant is both an economically important food crop and an ideal dicot model to investigate various physiological phenomena not possible in Arabidopsis thaliana. Due to the grea...

    Authors: Rogério F Carvalho, Marcelo L Campos, Lilian E Pino, Simone L Crestana, Agustin Zsögön, Joni E Lima, Vagner A Benedito and Lázaro EP Peres
    Citation: Plant Methods 2011 7:18
    Content type: Methodology Published on:

  30. The foundations for ethylene research were laid many years ago by researchers such as Lizada, Yang and Hoffman. Nowadays, most of the methods developed by them are still being used. Technological developments ...

    Authors: Inge Bulens, Bram Van de Poel, Maarten LATM Hertog, Maurice P De Proft, Annemie H Geeraerd and Bart M Nicolaï
    Citation: Plant Methods 2011 7:17
    Content type: Methodology Published on:

  31. Common bean (Phaseolus vulgaris L.) is a crop of economic and nutritious importance in many parts of the world. The lack of genomic resources have impeded the advancement of common bean genomics and thereby crop ...

    Authors: Claudia Díaz-Camino, Padmanaban Annamalai, Federico Sanchez, Aardra Kachroo and Said A Ghabrial
    Citation: Plant Methods 2011 7:16
    Content type: Methodology Published on:

  32. Virus-induced gene silencing (VIGS) is an effective technology for the analysis of gene functions in plants. Though there are many reports on virus vectors for VIGS in plants, no VIGS vectors available for Rosace...

    Authors: Shintarou Sasaki, Noriko Yamagishi and Nobuyuki Yoshikawa
    Citation: Plant Methods 2011 7:15
    Content type: Methodology Published on:

  33. Efficient high throughput screening systems of useful mutants are prerequisite for study of plant functional genomics and lots of application fields. Advance in such screening tools, thanks to the development ...

    Authors: Suk Weon Kim, Hye Jin Kim, Jong Hyun Kim, Yong Kook Kwon, Myung Suk Ahn, Young Pyo Jang and Jang R Liu
    Citation: Plant Methods 2011 7:14
    Content type: Methodology Published on:

  35. Safflower (Carthamus tinctorius L.) is a difficult crop to genetically transform being susceptible to hyperhydration and poor in vitro root formation. In addition to traditional uses safflower has recently emerge...

    Authors: Srinivas Belide, Luch Hac, Surinder P Singh, Allan G Green and Craig C Wood
    Citation: Plant Methods 2011 7:12
    Content type: Methodology Published on:

  36. In vivo detection of protein-bound genomic regions can be achieved by combining chromatin-immunoprecipitation with next-generation sequencing technology (ChIP-seq). The large amount of sequence data produced by t...

    Authors: Jose M Muiño, Kerstin Kaufmann, Roeland CHJ van Ham, Gerco C Angenent and Pawel Krajewski
    Citation: Plant Methods 2011 7:11
    Content type: Software Published on:

  37. Artificial chromosomes (ACs) are a promising next-generation vector for genetic engineering. The most common methods for developing AC constructs are to clone and combine centromeric DNA and telomeric DNA frag...

    Authors: Li Lin, Dal-Hoe Koo, Wenli Zhang, Joseph St Peter and Jiming Jiang
    Citation: Plant Methods 2011 7:10
    Content type: Methodology Published on:

  38. There is an increasing demand for renewable resources to replace fossil fuels. However, different applications such as the production of secondary biofuels or combustion for energy production require different...

    Authors: Guanwu Zhou, Gail Taylor and Andrea Polle
    Citation: Plant Methods 2011 7:9
    Content type: Research Published on:

  39. In the contexts of genomics, post-genomics and systems biology approaches, data integration presents a major concern. Databases provide crucial solutions: they store, organize and allow information to be queri...

    Authors: Sandra Dèrozier, Franck Samson, Jean-Philippe Tamby, Cécile Guichard, Véronique Brunaud, Philippe Grevet, Séverine Gagnot, Philippe Label, Jean-Charles Leplé, Alain Lecharny and Sébastien Aubourg
    Citation: Plant Methods 2011 7:8
    Content type: Database Published on:

  41. Plant viruses are useful expression vectors because they can mount systemic infections allowing large amounts of recombinant protein to be produced rapidly in differentiated plant tissues. Pepino mosaic virus ...

    Authors: Raquel N Sempere, Pedro Gómez, Verónica Truniger and Miguel A Aranda
    Citation: Plant Methods 2011 7:6
    Content type: Methodology Published on:

  43. Small RNAs emerged over the last decade as key regulators in diverse biological processes in eukaryotic organisms. To identify and study small RNAs, good and efficient protocols are necessary to isolate them, ...

    Authors: FlordeFátima Rosas-Cárdenas, Noé Durán-Figueroa, Jean-Philippe Vielle-Calzada, Andrés Cruz-Hernández, Nayelli Marsch-Martínez and Stefan de Folter
    Citation: Plant Methods 2011 7:4
    Content type: Methodology Published on:

  45. With the establishment of advanced technology facilities for high throughput plant phenotyping, the problem of estimating plant biomass of individual plants from their two dimensional images is becoming increa...

    Authors: Mahmood R Golzarian, Ross A Frick, Karthika Rajendran, Bettina Berger, Stuart Roy, Mark Tester and Desmond S Lun
    Citation: Plant Methods 2011 7:2
    Content type: Methodology Published on:

  46. Inkjet micropatterning is a versatile deposition technique with broad applications in numerous fields. However, its application in plant science is largely unexplored. Leaf expansion is one of the most importa...

    Authors: Lisheng Wang, Simon T Beyer, Quentin CB Cronk and Konrad Walus
    Citation: Plant Methods 2011 7:1
    Content type: Methodology Published on:

  47. Quantification of transcripts, proteins, or metabolites is straightforward when the factor used to normalize these values remains constant between samples. However, normalization factors often vary among sampl...

    Authors: Hiroshi Shimada, Takeshi Obayashi, Naoki Takahashi, Minami Matsui and Atsushi Sakamoto
    Citation: Plant Methods 2010 6:29
    Content type: Methodology Published on:

  48. Virus-induced gene silencing (VIGS) is an alternative reverse genetics tool for silencing of genes in some plants, which are difficult to transform. The pea early-browning virus (PEBV) has been developed as a ...

    Authors: Mette Grønlund, Anne Olsen, Elisabeth I Johansen and Iver Jakobsen
    Citation: Plant Methods 2010 6:28
    Content type: Methodology Published on:

  49. Programmed cell death (PCD) is an essential process for the life cycle of all multicellular organisms. In higher plants however, relatively little is known about the cascade of genes and signalling molecules r...

    Authors: Bennett Young, Raymond Wightman, Robert Blanvillain, Sydney B Purcel and Patrick Gallois
    Citation: Plant Methods 2010 6:27
    Content type: Methodology Published on:

  50. Gene silencing vectors based on Barley stripe mosaic virus (BSMV) are used extensively in cereals to study gene function, but nearly all studies have been limited to genes expressed in leaves of barley and wheat....

    Authors: Andrzej Pacak, Katrin Geisler, Bodil Jørgensen, Maria Barciszewska-Pacak, Lena Nilsson, Tom Hamborg Nielsen, Elisabeth Johansen, Mette Grønlund, Iver Jakobsen and Merete Albrechtsen
    Citation: Plant Methods 2010 6:26
    Content type: Methodology Published on:
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ISSN: 1746-4811