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  1. Sedentary endoparasitic cyst nematodes form a feeding structure in plant roots, called a syncytium. Syncytium formation involves extensive transcriptional modifications, which leads to cell modifications such ...

    Authors: Rinu Kooliyottil, Louise-Marie Dandurand, Joseph C. Kuhl, Allan Caplan and Fangming Xiao
    Citation: Plant Methods 2017 13:68
  2. Seed development in the angiosperms requires the production of a female gametophyte (embryo sac) within the ovule. Many aspects of female reproductive development in cereal crops are yet to be described, large...

    Authors: Laura G. Wilkinson and Matthew R. Tucker
    Citation: Plant Methods 2017 13:67
  3. Common cutworm (CCW; Spodoptera litura Fabricius) is a major leaf-feeding pest of soybean in Asia. The previous methods of measuring antixenosis against CCW using adult plant under field or net-room conditions we...

    Authors: Guangnan Xing, Kai Liu and Junyi Gai
    Citation: Plant Methods 2017 13:66
  4. Understanding root traits is a necessary research front for selection of favorable genotypes or cultivation practices. Root and tuber crops having most of their economic potential stored below ground are favor...

    Authors: Alfredo Delgado, Dirk B. Hays, Richard K. Bruton, Hernán Ceballos, Alexandre Novo, Enrico Boi and Michael Gomez Selvaraj
    Citation: Plant Methods 2017 13:65
  5. Plant glutathione S-transferases (GSTs, EC 2.5.1.18) are multifunctional enzymes involved in heavy metal cellular detoxification by conjugating the tripeptide (g-Glu-Cys-Gly) glutathione to heavy metals. Previous...

    Authors: Zhenzhen Cao, Renxiang Mou, Zhaoyun Cao, Xiaoyan Lin, Youning Ma, Zhiwei Zhu and Mingxue Chen
    Citation: Plant Methods 2017 13:64
  6. Condensed tannins (CT) are the most abundant secondary metabolite of land plants and can vary in abundance and structure according to tissue type, species, genotype, age, and environmental conditions. Recent i...

    Authors: Philip-Edouard Shay, J. A. Trofymow and C. Peter Constabel
    Citation: Plant Methods 2017 13:63
  7. Modern agriculture uses hyperspectral cameras that provide hundreds of reflectance data at discrete narrow bands in many environments. These bands often cover the whole visible light spectrum and part of the i...

    Authors: Abelardo Montesinos-López, Osval A. Montesinos-López, Jaime Cuevas, Walter A. Mata-López, Juan Burgueño, Sushismita Mondal, Julio Huerta, Ravi Singh, Enrique Autrique, Lorena González-Pérez and José Crossa
    Citation: Plant Methods 2017 13:62
  8. Photosynthesis can be roughly separated into biochemical and photochemical processes. Both are affected by drought and can be assessed by non-invasive standard methods. Gas exchange, which mainly assesses the ...

    Authors: Carlos Antônio Ferreira de Sousa, Dayane Silva de Paiva, Raphael Augusto das Chagas Noqueli Casari, Nelson Geraldo de Oliveira, Hugo Bruno Correa Molinari, Adilson Kenji Kobayashi, Paulo Cesar Magalhães, Reinaldo Lúcio Gomide and Manoel Teixeira Souza Jr.
    Citation: Plant Methods 2017 13:61
  9. Virus-induced gene silencing (VIGS) has been used in many plant species as an attractive post transcriptional gene silencing (PTGS) method for studying gene function either individually or at large-scale in a ...

    Authors: Bangjun Zhou and Lirong Zeng
    Citation: Plant Methods 2017 13:59
  10. There are numerous systems and techniques to measure the growth of plant roots. However, phenotyping large numbers of plant roots for breeding and genetic analyses remains challenging. One major difficulty is ...

    Authors: Lionel X. Dupuy, Gladys Wright, Jacqueline A. Thompson, Anna Taylor, Sebastien Dekeyser, Christopher P. White, William T. B. Thomas, Mark Nightingale, John P. Hammond, Neil S. Graham, Catherine L. Thomas, Martin R. Broadley and Philip J. White
    Citation: Plant Methods 2017 13:57
  11. In sorghum, the growth angle of nodal roots is a major component of root system architecture. It strongly influences the spatial distribution of roots of mature plants in the soil profile, which can impact dro...

    Authors: Dinesh C. Joshi, Vijaya Singh, Colleen Hunt, Emma Mace, Erik van Oosterom, Richard Sulman, David Jordan and Graeme Hammer
    Citation: Plant Methods 2017 13:56
  12. Tree pruning is a costly practice with important implications for crop harvest and nutrition, pest and disease control, soil protection and irrigation strategies. Investigations on tree pruning usually involve...

    Authors: F. M. Jiménez-Brenes, F. López-Granados, A. I. de Castro, J. Torres-Sánchez, N. Serrano and J. M. Peña
    Citation: Plant Methods 2017 13:55
  13. Automated plant phenotyping has been established as a powerful new tool in studying plant growth, development and response to various types of biotic or abiotic stressors. Respective facilities mainly apply no...

    Authors: Henning Tschiersch, Astrid Junker, Rhonda C. Meyer and Thomas Altmann
    Citation: Plant Methods 2017 13:54
  14. Low field NMR has been used to investigate water status in various plant tissues. In plants grown in controlled conditions, the method was shown to be able to monitor leaf development as it could detect slight...

    Authors: Maja Musse, Laurent Leport, Mireille Cambert, William Debrandt, Clément Sorin, Alain Bouchereau and François Mariette
    Citation: Plant Methods 2017 13:53
  15. Plant water resource management is one of the main future challenges to fight recent climatic changes. The knowledge of the plant water content could be indispensable for water saving strategies. Terahertz spe...

    Authors: Lorenzo Baldacci, Mario Pagano, Luca Masini, Alessandra Toncelli, Giorgio Carelli, Paolo Storchi and Alessandro Tredicucci
    Citation: Plant Methods 2017 13:51
  16. Precise control of gene expression is essential to synchronize plant development with the environment. In perennial plants, transcriptional regulation remains poorly understood, mainly due to the long time req...

    Authors: José M. Ramos-Sánchez, Paolo M. Triozzi, Alicia Moreno-Cortés, Daniel Conde, Mariano Perales and Isabel Allona
    Citation: Plant Methods 2017 13:50
  17. Within the Brassicaceae, six species from the genus Brassica are widely cultivated throughout the world as oilseed, condiment, fodder or vegetable crops. The genetic relationships among the six Brassica species a...

    Authors: Joshua C. O. Koh, Denise M. Barbulescu, Sally Norton, Bob Redden, Phil A. Salisbury, Sukhjiwan Kaur, Noel Cogan and Anthony T. Slater
    Citation: Plant Methods 2017 13:49
  18. In order to better understand and improve the mode of action of agrochemicals, it is useful to be able to visualize their uptake and distribution in vivo, non-invasively and, ideally, in the field. Here we exp...

    Authors: Elizabeth Noble, Sunil Kumar, Frederik G. Görlitz, Chris Stain, Chris Dunsby and Paul M. W. French
    Citation: Plant Methods 2017 13:48
  19. Hyperspectral imaging is an emerging means of assessing plant vitality, stress parameters, nutrition status, and diseases. Extraction of target values from the high-dimensional datasets either relies on pixel-...

    Authors: Uwe Knauer, Andrea Matros, Tijana Petrovic, Timothy Zanker, Eileen S. Scott and Udo Seiffert
    Citation: Plant Methods 2017 13:47
  20. Perennial ryegrass (Lolium perenne L.) is an important temperate grass used for turf and forage purposes. With the increasing accumulation of genomic and transcriptomic data of perennial ryegrass, an efficient pr...

    Authors: Guohui Yu, Qiang Cheng, Zheni Xie, Bin Xu, Bingru Huang and Bingyu Zhao
    Citation: Plant Methods 2017 13:46
  21. Chlorophyll fluorescence (CF) is a key indicator to study plant physiology or photosynthesis efficiency. Conventionally, CF is characterized by fluorometers, which only allows ensemble measurement through wide...

    Authors: Yi-Chin Tseng and Shi-Wei Chu
    Citation: Plant Methods 2017 13:43
  22. Binding of transcription factors to their target sequences is a primary step in the regulation of gene expression and largely determines gene regulatory networks. Chromatin immunoprecipitation (ChIP) is an ind...

    Authors: Jeong Hwan Lee, Suhyun Jin, Sun Young Kim, Wanhui Kim and Ji Hoon Ahn
    Citation: Plant Methods 2017 13:42
  23. The main aim of this study was to improve fungal resistance in bread wheat via transgenesis. Transgenic wheat plants harboring barley chitinase (chi26) gene, driven by maize ubi promoter, were obtained using biol...

    Authors: Hala F. Eissa, Sameh E. Hassanien, Ahmed M. Ramadan, Moustafa M. El-Shamy, Osama M. Saleh, Ahmed M. Shokry, Mohamed Abdelsattar, Yasser B. Morsy, Maher A. El-Maghraby, Hussien F. Alameldin, Sabah M. Hassan, Gamal H. Osman, Hesham T. Mahfouz, Gharib A. Gad El-Karim, Magdy A. Madkour and Ahmed Bahieldin
    Citation: Plant Methods 2017 13:41
  24. Positron imaging can be used to non-destructively visualize the dynamics of a positron-emitting radionuclide in vivo, and is therefore a tool for understanding the mechanisms of nutrient transport in intact pl...

    Authors: Nobuo Suzui, Yong-Gen Yin, Satomi Ishii, Hitoshi Sekimoto and Naoki Kawachi
    Citation: Plant Methods 2017 13:40
  25. Detection of plant diseases in a fast and simple way is crucial for timely disease control. Conventionally, plant diseases are accurately identified by DNA, RNA or serology based methods which are time consumi...

    Authors: Chu Zhang, Xuping Feng, Jian Wang, Fei Liu, Yong He and Weijun Zhou
    Citation: Plant Methods 2017 13:39
  26. Plant density and its non-uniformity drive the competition among plants as well as with weeds. They need thus to be estimated with small uncertainties accuracy. An optimal sampling method is proposed to estima...

    Authors: Shouyang Liu, Fred Baret, Denis Allard, Xiuliang Jin, Bruno Andrieu, Philippe Burger, Matthieu Hemmerlé and Alexis Comar
    Citation: Plant Methods 2017 13:38
  27. The unicellular green alga, Chlamydomonas reinhardtii, is a classic model for studying flagella and biofuel. However, precise gene editing, such as Clustered Regularly Interspaced Short Palindromic Repeats (CRISP...

    Authors: Xi Cheng, Gai Liu, Wenting Ke, Lijuan Zhao, Bo Lv, Xiaocui Ma, Nannan Xu, Xiaoling Xia, Xuan Deng, Chunlei Zheng and Kaiyao Huang
    Citation: Plant Methods 2017 13:36
  28. The Photochemical Reflectance Index (PRI) calculated from narrow-band spectral reflectance data is a vegetation index which is increasingly used as an indicator of photosynthetic activity. The leaf-level link ...

    Authors: Matti Mõttus, Rocío Hernández-Clemente, Viljami Perheentupa and Vincent Markiet
    Citation: Plant Methods 2017 13:35
  29. Reduction in rice yield caused by high temperature-induced spikelet sterility has been a serious concern in rice production. To date, several screening methods have been used, although their reproducibility i...

    Authors: Makoto Hakata, Hiroshi Wada, Chisato Masumoto-Kubo, Ryo Tanaka, Hiroyuki Sato and Satoshi Morita
    Citation: Plant Methods 2017 13:34
  30. Archegoniates (bryophytes, ferns and gymnosperms), such as the moss Physcomitrella patens, possess freely motile sperm cells (spermatozoids) which reach the egg cell via surface water. Although these motile flage...

    Authors: Nelly A. Horst and Ralf Reski
    Citation: Plant Methods 2017 13:33
  31. PTI and ETI are the two major defence mechanisms in plants. ETI is triggered by the detection of pathogen effectors, or their activity, in the plant cell and most of the time involves internal receptors known ...

    Authors: Cyril Brendolise, Mirco Montefiori, Romain Dinis, Nemo Peeters, Roy D. Storey and Erik H. Rikkerink
    Citation: Plant Methods 2017 13:32
  32. Chloroplasts play a great role for sustained wellbeing of life on the planet. They have the power and raw materials that can be used as sophisticated biological factories. They are rich in energy as they have ...

    Authors: Muhamed Adem, Dereje Beyene and Tileye Feyissa
    Citation: Plant Methods 2017 13:30
  33. The ability to target and manipulate protein-based cellular processes would accelerate plant research; yet, the technology to specifically and selectively target plant-expressed proteins is still in its infanc...

    Authors: André C. Velásquez, Kinya Nomura, Max D. Cooper, Brantley R. Herrin and Sheng Yang He
    Citation: Plant Methods 2017 13:29
  34. Sugarcane (Saccharum spp.) is the main raw material for sugar and ethanol production. Among the abiotic stress, drought is the main one that negatively impact sugarcane yield. Although gene expression analysis th...

    Authors: Larissa Mara de Andrade, Michael dos Santos Brito, Rafael Fávero Peixoto Junior, Paulo Eduardo Ribeiro Marchiori, Paula Macedo Nóbile, Alexandre Palma Boer Martins, Rafael Vasconcelos Ribeiro and Silvana Creste
    Citation: Plant Methods 2017 13:28
  35. Endoreduplication, the process of DNA replication in the absence of cell division, is associated with specialized cellular function and increased cell size. Genes controlling endoreduplication in tomato fruit ...

    Authors: F. Parker E. Laimbeer, Sarah H. Holt, Melissa Makris, Michael Alan Hardigan, C. Robin Buell and Richard E. Veilleux
    Citation: Plant Methods 2017 13:27
  36. Chlorophyll content decreases in plants under stress conditions, therefore it is used commonly as an indicator of plant health. Arabidopsis thaliana offers a convenient and fast way to test physiological phenotyp...

    Authors: Ying Liang, Daisuke Urano, Kang-Ling Liao, Tyson L. Hedrick, Yajun Gao and Alan M. Jones
    Citation: Plant Methods 2017 13:26
  37. Peanut (Arachis hypogaea) production is largely affected by a variety of abiotic and biotic stresses, including the root-knot nematode (RKN) Meloidogyne arenaria that causes yield losses worldwide. Transcriptome ...

    Authors: Larissa Arrais Guimaraes, Bruna Medeiros Pereira, Ana Claudia Guerra Araujo, Patricia Messenberg Guimaraes and Ana Cristina Miranda Brasileiro
    Citation: Plant Methods 2017 13:25
  38. Phenotyping is a critical component of plant research. Accurate and precise trait collection, when integrated with genetic tools, can greatly accelerate the rate of genetic gain in crop improvement. However, e...

    Authors: Hsiang Sing Naik, Jiaoping Zhang, Alec Lofquist, Teshale Assefa, Soumik Sarkar, David Ackerman, Arti Singh, Asheesh K. Singh and Baskar Ganapathysubramanian
    Citation: Plant Methods 2017 13:23
  39. Random insertional mutagenesis of Chlamydomonas reinhardtii using drug resistance cassettes has contributed to the generation of tens of thousands of transformants in dozens of labs around the world. In many inst...

    Authors: Steve V. Pollock, Bratati Mukherjee, Joanna Bajsa-Hirschel, Marylou C. Machingura, Ananya Mukherjee, Arthur R. Grossman and James V. Moroney
    Citation: Plant Methods 2017 13:22
  40. The maize male inflorescence (tassel) produces pollen necessary for reproduction and commercial grain production of maize. The size of the tassel has been linked to factors affecting grain yield, so understand...

    Authors: Joseph L. Gage, Nathan D. Miller, Edgar P. Spalding, Shawn M. Kaeppler and Natalia de Leon
    Citation: Plant Methods 2017 13:21
  41. The liverwort Marchantia polymorpha occupies a crucial position in land plant evolution and provides the opportunity to investigate adaptations to a terrestrial plant life style. Marchantia reverse genetic analys...

    Authors: Sarah Kopischke, Esther Schüßler, Felix Althoff and Sabine Zachgo
    Citation: Plant Methods 2017 13:20

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