 Research
 Open access
 Published:
TasselNet: counting maize tassels in the wild via local counts regression network
Plant Methods volumeÂ 13, ArticleÂ number:Â 79 (2017)
Abstract
Background
Accurately counting maize tassels is important for monitoring the growth status of maize plants. This tedious task, however, is still mainly done by manual efforts. In the context of modern plant phenotyping, automating this task is required to meet the need of largescale analysis of genotype and phenotype. In recent years, computer vision technologies have experienced a significant breakthrough due to the emergence of largescale datasets and increased computational resources. Naturally imagebased approaches have also received much attention in plantrelated studies. Yet a fact is that most imagebased systems for plant phenotyping are deployed under controlled laboratory environment. When transferring the application scenario to unconstrained infield conditions, intrinsic and extrinsic variations in the wild pose great challenges for accurate counting of maize tassels, which goes beyond the ability of conventional image processing techniques. This calls for further robust computer vision approaches to address infield variations.
Results
This paper studies the infield counting problem of maize tassels. To our knowledge, this is the first time that a plantrelated counting problem is considered using computer vision technologies under unconstrained fieldbased environment. With 361 field images collected in four experimental fields across China between 2010 and 2015 and corresponding manuallylabelled dotted annotations, a novel Maize Tassels Counting (MTC) dataset is created and will be released with this paper. To alleviate the infield challenges, a deep convolutional neural networkbased approach termed TasselNet is proposed. TasselNet can achieve good adaptability to infield variations via modelling the local visual characteristics of field images and regressing the local counts of maize tassels. Extensive results on the MTC dataset demonstrate that TasselNet outperforms other stateoftheart approaches by large margins and achieves the overall best counting performance, with a mean absolute error of 6.6 and a mean squared error of 9.6 averaged over 8 test sequences.
Conclusions
TasselNet can achieve robust infield counting of maize tassels with a relatively high degree of accuracy. Our experimental evaluations also suggest several good practices for practitioners working on maizetassellike counting problems. It is worth noting that, though the counting errors have been greatly reduced by TasselNet, infield counting of maize tassels remains an open and unsolved problem.
Background
We consider the problem of counting maize tassels from images captured in the field using computer vision. Maize tassels are the male flowers of maize plants. The emergence of tassels indicates the arrival of the reproductive stage. During this stage, the total tassel number is an important cue to monitor the growth status of maize plants. It is closely related to the growth stage [1] and yield potential [2]. In practice, counting maize tassels still mainly depends on human efforts, which is inefficient and fallible. Such a tedious task should be replaced by machines in modern plant phenotyping.
To meet the need of largescale and highthroughput analysis in plant phenotyping, imagebased techniques provide a feasible, lowend, and efficient solution and have thus received much attention recently [2,3,4,5]. However, most practitioners and researchers still conduct experiments under controlled artificial environment. Although indoor experiments do simplify the process of image processing and advance our knowledge regarding the link between genotype and phenotype, ultimately plant phenotyping must be transferred to realworld scenarios, such as in the field or greenhouse [6]. Unfortunately, intrinsic and extrinsic variations in the wild field render the understanding and processing of fieldbased images a challenging task. Such challenges become more serious in the problem of infield counting of maize tassels. As shown in Fig.Â 1, these challenges can largely boil down to the variations in the fieldbased environment:

Maize tassels emerge suddenly and vary significantly in shape and size as plants grow over time;

Different cultivars of maize plants exhibit different appearance variations, such as colour and texture;

Illumination changes dramatically due to different weather conditions, especially during the sunny day;

The wind, imaging angle and perspective distortions cause various pose variations;

Occlusions occur frequently, which renders the difficulty for counting even for a human expert;

The cluttered background make visual patterns of maize tassels diverse and misleading;

The quality of images degrades because of the dust or rain drops on the camera lens;

Textural patterns also change essentially due to different flowering status.
It is worth noting that these challenges are not only specific to maize tassels but also applicable to a wide species of plants. It is inevitable to face these infield variations before deploying plant phenotyping systems in the wild.
Though efforts have been made to tackle above problems and have achieved a moderate degree of success, the precision of the stateoftheart tassel detection method is still below 50% [2]. This may be largely due to the inherent limitation of the nonmaximum suppression mechanism within object detection [7]â€”it cannot appropriately distinguish overlapping objects. Such a mechanism poses problems for accurate maize tassels detection because overlaps between different tassels are common patterns in the field. We have to ask: is the object detection the best way to count maize tassels? From a point of view of Computer Vision, the objective of object detection is to localise individual instances and output their corresponding bounding boxes. Since the locations of objects are identified, it is easy to derive the number of instances. However, the number of instances actually has nothing to do with the location. If one only cares about estimating the total number of instances, the problem is another important research topic in Computer Visionâ€”object counting. In this paper, we show that it is better to formulate the task of maize tassels counting as a typical counting problem, rather than a detection one. In fact, object detection is generally more difficult to solve than object counting.
Nevertheless, object counting remains a known challenging task [8, 9], in both Plant Science and Computer Vision communities. Three sessions of Leaf Counting Challenge have been held in conjunction with the Computer Vision Problems in Plant Phenotyping workshops (CVPPP2014 [10]/CVPPP2015 [11]/ CVPPP2017 [12]), expecting to showcase visual challenges for plant phenotyping. Many efforts are also made in recent years in Computer Vision to improve the counting precision of crowds [13, 14], cells [15, 16], cars [17, 18], and animals [19]. However, little attention has been paid to plantsrelated counting tasks. To our knowledge, only two published papers considered counting problems relating to plants. Giuffrida et al. [20] proposed a learningbased approach to count leaves in rosette plants. Rahnemoonfar and Sheppard [21] presented a deep simulated learning approach to count tomato images. A limitation is that both papers only report their results on potted plants, which is far different from fieldbased scenarios. In contrast, our experiments use images captured exactly under unconstrained infield environment, leading to a more challenging situation and a more reasonable experimental evaluation.
According to the taxonomy of [22], existing object counting approaches can be classified into three categories: counting by clustering, counting by detection, and counting by regression. The countingbyclustering approaches often rely on the extraction of motion features (see [23] for example), which is not applicable to the plants because the motion of plants is almost unobservable within limited time. In addition, the countingbydetection approaches [24, 25] tend to suffer in crowded scenes with significant occlusions, so this type of method is also not a good choice for our problem. In fact, the transductive principle suggests never to solve a harder problem than the target application necessitates [26]. As a consequence, recent countingbyregression models [13, 15, 17] have demonstrated that it is indeed unnecessary to detect or segment individual instances when estimating their counts. In particular, the key component of modern countingbyregression approaches is the introduction of the density map by Lempitsky and Zisserman [15]. Objects in an image are described by a density map given dot annotations. During the prediction, each object will be assigned a density that sums to 1, so the total number of objects can be reflected by summing over the whole density map. Overlapping objects are naturally taken into account in this paradigm.
Further, countingbyregression approaches can be divided into two subcategories: global regression [13, 20, 27] and local regression [14, 15, 19, 28]. Some early attempts try to regress the global image count directly via either Gaussian regression [13] or regression forest [27]. Chen et al. [29] estimates the local image count using a multioutput ridge regression model. Lempitsky and Zisserman [15], however, chooses to regress the local density map, which is found to be more effective than regressing just the global/local image count. At this time, although a moderate degree of counting accuracy is achieved, the performance is limited by the power of the feature representation. Such a circumstance eases in the era of deep learning, when the feature could be learnt and adjusted given a specific problem. The first deep counting approach can be found in [14], where the problem is addressed by regressing a local density map with deep networks. In fact, most subsequent deep counting approaches also follow this paradigm [18, 19, 30]. More recently, Cohen et al. [28] presents a somewhat different idea that regresses the local subimage count with deep networks. We also take inspirations from Cohen et al. [28]. Readers can refer to Sindagi and Patel [31] for a comprehensive survey to the recent advance of deep networks in counting problems.
To better address aforementioned challenges, we follow the idea of counting by regression and present in this paper a deep convolutional neural network [32] (CNN)based approach for maize tassels counting, which is referred to TasselNet. Deep networks are famous due to their excellent nonlinear modelling ability and large model capacity, which is important for capturing diverse and complex visual patterns in the field. Notice that plants are like selfchanging systems, the physical size of maize tassels in images vary significantly over time. This is what makes the problem of maize tassels counting different from other conventional counting problems in Computer Vision (the physical size of pedestrians, cells or cars in images remains unchanged or at least identical), and consequently, renders difficulties to describe the density map of maize tassels. To address this, in contrast to [15, 18] that either regress the global density map or the local density map, we propose to regress the local count computed from the density map. After merging and normalizing all local counts, our model outputs a count map similar to the groundtruth density map. The final count of maize tassels is computed by summing over the whole count map. FigureÂ 2 illustrates our main technical pipeline.
To validate the effectiveness of the proposed approach, a novel Maize Tassel Counting (MTC) dataset is constructed and will be released together with this paper. Our MTC dataset contains 361 images chosen from 16 image sequences. These sequences are collected from 2010 to 2015, covering 4 different experimental fields across China. All challenges described in Fig.Â 1 are involved in this dataset. The number of maize tassels in images varies between 0 and around 100. Following the standard annotation used in objection counting problems [15], a single dot is manually assigned for each maize tassel. We hope such a dataset could be used as a benchmark for evaluating infield counting approaches and could draw attention from practitioners working in this area to attach importance to these infield challenges.
Extensive evaluations are performed on the MTC dataset. Experimental results demonstrate that TasselNet outperforms other stateoftheart methods and significantly reduces the counting errors by large margins. Moreover, based on the experimental results, we also suggest several good practices for infield counting problems.
The contributions of this paper are multifold:

A novel counting problem of maize tassels whose sizes are selfchanging over time. To the best of our knowledge, this is the first time that a plantrelated counting problem is considered under unconstrained field conditions;

A challenging MTC dataset with 361 field images and corresponding manuallylabelled dotted annotations;

TasselNet: an effective deep CNNbased solution for infield counting of maize tassels via local counts regression.
Methods
Experimental fields and imaging devices
16 independent timeseries image sequences are collected from four different experimental fields across China between 2010 and 2015. Four experimental fields are located in Zhengzhou, Henan Province, China, Taian, Shandong Province, China, Gucheng, Hebei Province, China, and Jalaid, Sinkiang Autonomous Region, China, respectively. Six cultivars of maize plants are involved, including Jundan No. 20, Nongda No. 108, Wuyue No. 3, Zhengdan No. 32, Jidan No. 20, and Tianlong No. 9. FigureÂ 3 show the experimental fields and imaging devices. The main components of the imaging device include a highresolution CCD digital camera (E450 Olympus), a lowresolution monitoring device, a 3G wireless data transmission system, as well as several solar panels used for power supply. When an image is captured, it will be transmitted into a remote server, and then users can access the image data. Readers can refer to [33] for a detailed introduction of our imaging device. The focal length of the camera is fixed to 16mm. Images were taken every one hour from 9:00 to 16:00 from a fivemetersheight vertical view (four meters for Gucheng sequences). The original image resolutions are 3648Â Ã—Â 2736 pixels for Zhengzhou and Taian sequences, 4272Â Ã—Â 2848 pixels for Gucheng sequences, and 3456Â Ã—Â 2304 pixels for Jalaid sequences.
Maize tassels counting dataset
Given 16 independent time series image sequences, images captured from the tasselling stage to the flowering stage are considered in our MTC dataset. In particular, according to the variability each sequence presents, 8â€“45 images are manually chosen from each sequence. If extrinsic conditions, such as weather conditions or the wind, change dramatically, more images will be chosen in one day, otherwise only 1 or 2 images are chosen. Such a sampling strategy is used with the motivation to avoid repetitive samples as much as possible, because images captured in one day usually do not exhibit many variations. However, the ability to model various data variabilities is much more important than blindly fitting a large number of repetitive samples for an effective computer vision approach. Thus, 361 field images in all are chosen to construct the MTC dataset. The MTC dataset is divided into the training set, validation set and test set. The training set and validation set share the same image sequences, while the test set uses different image sequences to enable a reasonable evaluation. Such an intentional setting is motivated by the fact that images in one sequence are often highly correlated, it is thus inappropriate to place them into both the training and test stages. TableÂ 1 summarises the information of the MTC dataset. Overall, we have 186 images for training and validation and 175 images for test.
We also follow the standard annotation paradigm that manually provides each tassel with a dot annotation [15]. Indeed, dotting is regarded as a natural way to count for humans. It not only gives the raw counts of the image but also proffer the information how objects spatially distribute. FigureÂ 4 show four example images with the dotted annotations.
Local counts regression network
In this section we describe our proposed local counts regression network and show how to use it to address effectively the infield counting problem of maize tassels.
The highlevel idea of counting by regression is simple: given an image I and a regression target T, the goal is to seek some kind of regression function F so that \(T\approx F(I)\). Standard solutions are to regress explicitly the raw counts in an image [13] (T is the global counts) or to regress implicitly the density map of an image [15] (T becomes a density map, and the counts can be acquired by integrating over the entire density map). However, as what we will show in our experiments, both solutions are not effective for maize tassels counting. The reason may boil down to the heterogeneity of maize tassels. As shown in Fig.Â 4, maize tassels exhibit uncertain poses and varying sizes, making them hard to be described by only a global image representation or a density map given only dotted annotations. Indeed, this is what makes maize tassels counting different from other standard counting problems.
Inspired by a recent idea of redundant counting [28], we propose to regress the local counts \(T_l\) to address the counting problem of maize tassels. \(T_l\) refers to the object count within a small subimage \(I_s\). The proposed local regression has several benefits: (1) local characteristics are easier to be modelled than the global ones; (2) by regressing the local counts, we avoid the hard problem of dense perpixel learning (compared to estimating the local density map); (3) by sampling small image patches, we can have access to a large number of training data, allowing us to train a highcapacity model. In particular, we consider the regression function F should be powerful enough so that it can appropriately capture those heterogeneous infield variations. Inspired by the recent success of deep convolutional neural networks (CNNs) in visual recognition [32, 34], we choose to formulate F in a deep CNNbased framework. The goal is thus to recover \(T_l\) with a set of nonlinear transformations F given \(I_s\), i.e., \(T_l\approx F(I_s)\). FigureÂ 5 compares the conceptual difference of different regression goals. During the prediction, subimages are densely sampled from a test image, and F will assign a local count to each subimage patch. The final count of the image can be recovered by aggregating all subimage counts into a count map with the same size of the test image, and a perpixel normalisation step is performed to each pixel by dividing the number of subimages that cast a prediction in it.
Regression target
Different regression targets imply different regression strategies, so how to define the regression target is the first and the most important step. In this paper, we first follow the standard way that generates the ground truth density by placing a Gaussian at each dot annotation [15]. Formally, given a groundtruth dot image Y, a density map D can be defined as \(D=G*Y\), where G denotes a twodimensional Gaussian kernel parametrised by \(\sigma\), and \(*\) indicates the convolution operation. Obviously, D is generated by performing Gaussian smoothing on Y. FigureÂ 6 show an example of D given corresponding dotted annotations. It is worth noting that the summation of the density map is a decimal. The reason is that, when dots are close to the image boundary, their Gaussian probability will be partly outside the image, but this definition naturally takes a fraction of an objection into account.
However, in contrast to [15, 18], we do not regard D as our regression target (we will show later in our experiments that the density map is too harsh as the regression target) but use the local counts integrated from the density map. In other words, some approaches produce a 2D network output, e.g., [18] outputs an 18Â Ã—Â 18 result, while TasselNet produces specifically an 1Â Ã—Â 1 output. The final count map is generated by scanning and combining scans at each location. If let D(x,Â y) be the pixellevel count of D at the location (x,Â y), then the regression target of local counts \(T_l^i\) for the ith subimage \(I_s^i\) can be defined:
where \(S_i\) denotes the set of pixel locations of \(I_s^i.\)
Network architecture
The network architecture closely relates to the model capacity, and the model capacity is also a key factor that affects the counting performance. Motivated by the leading role of CNNs in Computer Vision, in this paper we evaluate three typical network architectures: a lowcapacity 4layer model identical to the seminal LeNet architecture [35], a mediumcapacity 7layer model similar to the AlexNet architecture [32], as well as a highcapacity 16layer model sharing the same spirit of the VGGVG16Net [34].
We follow the modern CNN design principle used in [34]: adopting only small 3Â Ã—Â 3 convolution kernels with 1pixel padding to preserve the size of the tensor, doubling the number of feature maps in the higher layers to compensate the loss of the spatial information after the max pooling operation, synthesizing learnt features with two extra fullyconnected layers, and using the ReLU function after each convolutional/fullconnected layer. FigureÂ 7 shows three architectures with a basic input size of 32Â Ã—Â 32 subimage. The number of parameters within the LeNetlike, AlexNetlike and VGGVD16Netlike architectures are about \(1.4\times 10^5\), \(2.5\times 10^5\) and \(2.4\times 10^6\), respectively.
Loss function
The learning of the regression network should be driven by a loss function. In this paper, we evaluate three typical loss functions used in regression problems. They are \(\ell _1\) loss, \(\ell _2\) loss, and Huber loss. \(\ell _1\) loss and \(\ell _2\) loss take the form
where L1 and L2 denote the \(\ell _1\) and \(\ell _2\) loss functions, respectively, M is the number of training subimages, and \(a_i\) is the residual that measures the difference between the regressed count and the ground truth count for the ith subimage, i.e., \(a_i=F(I_s^i)T_l^i\). Empirically, \(\ell _1\) loss is considered more robust to noise than \(\ell _2\) loss. Apart from these two standard choices, another widelyused loss function in robust regression is the Huber loss, which is defined as
where
and \(\delta\) is a userdefined constant. Huber loss can be viewed as an integration of \(\ell _1\) and \(\ell _2\) losses. We will show later in our experiments that \(\ell _1\) loss is the most effective one for maize tassels counting.
Merging and normalizing subimage counts
During the prediction, TasselNet will scan the image in a sliding window manner with a stride of \(s_e\). Each window corresponds to a subimage with size of \(r\times r\). For each subimage, TasselNet regresses a local count indicating the number of tassels within the subimage. Assume that K subimages in all are processed. Since each subimage may be counted multiple times due to the denselysampled mechanism, the final count of maize tassels cannot be directly computed by simply summing over all K local counts. To address this, here we develop a merging strategy to map K local counts back to the original test image. The normalization strategy is similar to the procedures introduced in [18, 28]. Assume that the kth subimage count is \(c_k\), we average \(c_k\) into every pixel of the kth subimage, so the count of each pixel takes up \(\frac{c_k}{r^2}\) (the sum of pixellevel counts still equals to \(c_k\)). In this way, a count map C with the same resolution of the test image can be consequently constructed by mapping the \(r\times r\) local count map back to the same location where the subimage is sampled. FigureÂ 2 illustrates this process. Finally, by constructing a normalisation image P that records how many times each pixel is counted, the final count of the image c can be computed as
where C(x,Â y) and P(x,Â y) denote the value of C and P at the location (x,Â y).
Implementation and learning details
We implement TasselNet based on MatConvNet [36]. Original highresolution images are resized to their 1/8 sizes to reduce computational burden. During training, we densely crop \(r\times r\) subimages with a stride of \(s_r\) from 186 images belonging to the training and validation sequences of MTC dataset. We perform a random shuffling of these subimages, 90% subimages are used for training, and the rest for validation. Before feeding the image samples into the network, each subimage is preprocessed by mean subtraction (the mean is computed from the training subset).
Note that, no data augmentation is performed when reporting the results, because we consider fieldbased conditions already cover various scenarios (the diversity of training data can be guaranteed). One may further improve the network performance with random rotation, flipping and cropping of training images. It also should be kept in mind that the ground truth counts may change accordingly.
The parameters of the convolution kernels are initialised with the improved Xavier method [37]. The standard stochastic gradient descent is used to optimise the network parameters. The learning rate is initially set to 0.01, and is decreased by a factor of 10 after 5 epochs and further decreased by a factor of 10 after another 10 epochs. Thus, we train TasselNet for 25 epochs in all. To allow the gradient to backpropagate easily from the output layer to the input layer, we add a batch normalisation layer [38] after each convolutional layer before ReLU. The training time of TasselNet varies from half a day to 2 days depending on the number of training samples and the network architecture used. The prediction time for each image takes about 2.5 seconds (Matlab 2016a, OS: Ubuntu 14.04 64bit, CPU: Intel E52630 2.40GHz, GPU: Nvidia GeForce GTX TITAN X, RAM: 64 GB).
TableÂ 2 summarises the default parameters used in our experiments. When \(s_r=8\), 355,473 and 31,167 subimages are densely sampled and ready for training and validation, respectively.
Results and discussion
We evaluate the effectiveness of TasselNet on the test sequences of MTC dataset. It is worth noting that Jalaid2015_2 and Jalaid2015_3 are two very challenging sequences. As shown in Fig.Â 8, images in the Jalaid2015_2 sequence suffer from dramatic illumination variations (Jalaid locates in a highlatitude area), and maize tassels in the Jalaid2015_2 sequence exhibit extremely crowded distributions. Extensive experiments are conducted to investigate key factors that affect the counting performance and to compare TasselNet against other stateoftheart approaches. Based on the experimental results, we also suggest several good practices for practitioners working on infiled counting problems.
Evaluation metric
The mean absolute error (MAE) and the mean squared error (MSE) are used as the evaluation metrics to assess the counting performance. They take the form
where N denotes the number of test images, \(t_i\) is the ground truth count for the ith image (computed by summing over the whole density map), and \(c_i\) is the inferred image count for the ith image (computed as per Eq.Â (6)). MAE quantifies the accuracy of the estimates, and MSE assesses the robustness of the estimates. The lower these two measures are, the better the counting performance is.
Choices of different network architectures, number of training samples, loss functions, Gaussian kernel parameters, and subimage sizes
Here we perform extensive evaluations to justify our design choices. Notice that, in a principal way, the inclusion of specific design choices should be justified on the validation set. However, since we enforce the test set to be different sequences, the validation set thus exhibits a substantially different data distribution. Validating our design choices based on the validation set seems suboptimal. Instead, as a preliminary study, we direct report the counting performance on the test set to see how the variations of these design choices affect the final counting performance. Although with a little abuse, we demonstrate later that the performance of TasselNet with any design choice shows a notable improvement over other baseline approaches by large margins. Below we follow the default parameters setting when reporting experimental results unless a specific design choice is declared.
Network architecture
We first evaluate how the model capacity influences the results. As aforementioned, three network architectures of LeNetlike, AlexNetlike and VGGVD16Netlike TasselNet are considered. The learning curves of three models are shown in Fig.Â 9. It is clear that, the deeper the model uses, the lower the training error achieves. However, a lower training error does not imply a lower validation error for our problemâ€”the validation error of VGGVD16Netlike model shows obvious fluctuations and is also higher than the AlexNetlike model. Numerical results on the test set are shown in TableÂ 3. Experimental results demonstrate that the AlexNetlike architecture achieves the overall best counting performance, with a MAE of 6.6 and a MSE of 9.6. The inferiority of LeNet and VGGVD16Net may boil down to the low model capacity and the overfitting on the training data. This can be clearly observed in Fig.Â 9. The low model capacity of LeNetlike architecture shows a relatively high training error, which implies the data may be in the state of underfitting. FigureÂ 9 show that the VGGVD16Netlike architecture fits the training data well while exhibits a higher validation error (compared to the AlexNetlike architecture), which suggests the model may not generalize well. Based on these results, a moderately complex model seems sufficient for maize tassels counting. In following evaluations, the AlexNetlike architecture is used in TasselNet.
Further, it is worth noting that there exists some recent network architectures, such as ResNets [39] and DenseNets [40], that exhibit more powerful modelling ability than the three baseline architectures presented in this paper. One may find better counting performance using advanced architectures. We leave these explorations open at present.
Number of training samples
Here we investigate how the number of training samples affects the counting performance. We vary the sampling stride \(s_r\) using the range of values \(2^n, n=5, 4, 3, 2\), leading to \(2.37\times 10^4\), \(9.13\times 10^4\), \(3.56\times 10^5\), and \(1.41\times 10^6\) training subimages, respectively. Experimental results are listed in TableÂ 4. We observe that the number of training samples indeed plays a vital role: MAE decreases from 9.5 to 6.5 with increased training number of subimages. In addition, the overall performance between \(s_r=8\) and \(s_r=4\) is almost identical, implying that a moderate number of training subimages can already capture well the infield variations.
Loss function
Here we compare the effect of different loss functions. As aforementioned, \(\ell _1\) loss, \(\ell _2\) loss, and Huber loss are evaluated. Huber loss contains a free parameter \(\delta\), so we further add three variants of Huber loss when \(\delta =0.1\), \(\delta =1\), and \(\delta =10\). The lower \(\delta\) is, the more Huber loss looks like \(\ell _1\) loss. The higher \(\delta\) is, the more it looks like \(\ell _2\) loss. Results are shown in TableÂ 5. We observe that, there is no single loss can achieve consistently better results than other competitors over all test sequences. This may have something to do with the problem nature of maize tassels counting and specific data distributions of test sequences. Huber loss with \(delta=10\) shows better performance on the two challenging sequences, which suggests that Huber loss is indeed robust to noise but at a cost of sacrificing the ability to fit normal data samples (poor performance on the Taian2013_2 and Gucheng2014 sequences). Huber loss also has a problem that there is no principal way to choose an appropriate \(\delta\) (the performance degrades when \(delta=0.1\)). The counting performance of \(\ell _1\) loss and \(\ell _2\) loss is comparable, but \(\ell _1\) is generally more stable.
Gaussian kernel parameter
The sensitivity of Gaussian kernel parameter \(\sigma\) is further evaluated. Concretely, we set \(\sigma =4\), \(\sigma =8\), and \(\sigma =12\), respectively. The results are listed in TableÂ 6. We observe that the optimal \(\sigma\) for each test sequence is different. The reason perhaps is that a fixed \(\sigma\) cannot describe appropriately maize tassels of different sizes (due to different cultivars). Although the optimal counting performance cannot be achieved with a specific \(\sigma\), the counting performance with different \(\sigma\) does not vary significantly, which suggests that the mechanism of local counts regression is not that sensitive to specific choices of \(\sigma\). Empirically, one can set an appropriate \(\sigma\) by observing the Gaussian smoothing responses on the training set. The responses should fit the median size of maize tassels.
Subimage sizes
The influence of different subimage sizes is also analysed. We compare the performance of four settings, including \(r=16\), \(r=32\), \(r=64\), and \(r=96\). TableÂ 7 lists the results. According to the results, we again observe that the optimal performance for each test sequence does not correlate well with subimage sizes. We think this has something to do with specific tassel sizes in each sequences. In practice, drawing upon a relatively small (but not too small) subimage sizes is preferable. This is not just because one can densely sample a sufficient number of training samples but also because the variations within a small receptive field are easily modelled.
Comparison with the state of the art
To place TasselNet in the context of the state of the art, several wellestablished baseline approaches are chosen for comparison, they are:

JointSeg [41]: JointSeg is the stateoftheart tassel segmentation method. The number of object counts can be easily inferred from the segmentation results. We further perform some morphological operations as postcorrection to reduce the segmentation noises. This approach can be viewed as a countingbysegmentation baseline. It is not specially designed for a counting problem, but the comparison somewhat justify whether our problem could be addressed by a simple image processing technique.

mTASSEL [2]: mTASSEL is the stateoftheart tassel detection approach designed specifically for maize tassels. mTASSEL uses multiview representations to characterise the visual characteristics of tassels to achieve robust detection. This is a countingbydetection baseline.

GlobalReg [42]: GlobalReg directly regresses the global count of images. Offtheshelf fullyconnected deep activations extracted from a pretrained model are used as a holistic image representation. Then the global image feature is linearly mapped into a global object count by ridge regression. This is a global countingbyregression baseline.

DensityReg [15]: DensityReg is the seminal work that proposes the idea of density map regression. It predicts a count density for every pixel by optimising a socalled MESA distance. This is a global densitybased countingbyregression baseline.

CountingCNN (CCNN) [18]: CCNN is a stateoftheart object counting approach. It treats the local density map as the regression target and also uses a AlexNetlike CNN architecture. This is a local densitybased countingbyregression baseline.
Qualitative and quantitative results are shown in Fig.Â 10 and TableÂ 8, respectively. Results of TasselNet are reported using the default parameters setting, i.e., with the AlexNetlike architecture and suggested parameters. According to the results, we can make the following observations:

TasselNet outperforms other baseline approaches in 7 out of 8 test sequences and achieves the overall best counting performanceâ€”MAE and MSE are significantly lower than other competitors.

The poor performance of JointSeg and mTASSEL implies that the problem of infield counting of maize tassels cannot be solved by simple colourcuebased image segmentation or standard object detection.

Even a simple global regression can achieve comparable counting performance against mTASSEL in which the boundingboxlevel annotations are utilized. This suggests it is better to formulate the problem of maize tassels counting in a countingbyregression manner.

Regressing the global density map can also reduce the counting error effectively. However, it is hard to extend this idea to the deep CNNbased paradigm, because there is currently no dataset with thousands of labelled images samples to make the learning of deep networks tractable, especially in the plantrelated scenarios. Hence, DensityReg cannot enjoy the bonus brought by deep CNN, and the performance may be limited by the power of feature representation.

The performance of CCNN even falls behind the global regression baseline. In experiments we observe that CCNN performs poorly when given an image with just a few tassels of different types. Compared to regressing local counts as in TasselNet, CCNN needs to fit harsher pixellevel ground truth density, so it likely suffers in the vague definition of density map due to different tassel sizes. This may explain why local density regression does not work when given varying object sizes like maize tassels.

Qualitative results in Fig.Â 10 show that TasselNet can give reasonable approximations to the ground truth density maps. In most cases, the estimated counts are similar to the ground truth counts. However, there also exists some circumstances that TasselNet cannot give an accurate prediction. The last row in Fig.Â 10 show three failure cases: (1) when the image is captured under extremely strong illuminations, highlight regions of leaves will contribute to several fake responses; (2) if maize tassels present longtailed shapes in images, the longtailed parts only receive partial local counts, resulting in a underestimate situation; (3) the extremely crowded scene is also beyond the ability of TasselNet. To alleviate these issues, one may consider to add extra training data that contain the extremely crowded scenarios. Alternatively, since the training sequences and test sequences exhibit more or less different data distributions, it may be possible to use domain adaptation [43] to fill the last few percent of difference between sequences. We leave these as the future explorations of this work.
As a summary of our evaluations, we suggest the following good practices for maizetassellike infield counting problems:

1.
Try the idea of counting by regression if the objects exhibit significant occlusions.

2.
Try local counts regression if the physical size of objects varies dramatically.

3.
Use a relatively small subimage size so that a sufficient number of training samples could be sampled.

4.
It is safe to use a moderately complex deep model.

5.
Try \(\ell _1\) loss first to achieve a robust regression.
Conclusions
In this paper, we rethink the problem nature of infield counting of maize tassels and novelly formulates the problem as an object counting task. A tailored MTC dataset with 361 field images captured during 6 years and corresponding manuallylabelled dotted annotations is constructed. An effective deep CNNbased solution, TasselNet, is also presented to count effectively maize tassels via local counts regression. We show that local counts regression is particularly suitable for counting problems whose ground truth density maps cannot be precisely defined. Extensive experiments are conducted to justify the effectiveness of our proposition. Results show that TasselNet achieves the stateoftheart performance and outperforms previous baseline approaches by large margins.
For future work, we will continue to enrich the MTC dataset, because the training data are always the key to the good performance, especially the data diversity. In addition, we will explore the feasibility to improve the counting performance in the context of domain adaptation, because the adaptation of object counting problems still remains an open question. Infield counting of maize tassels is a challenging problem, not only because the unconstrained natural environment but also because the selfchanging rule of plants growth. We hope this paper could attract interests of both Plant Science and Computer Vision communities and inspires further studies to advance our knowledge and understanding towards the problem.
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Authors' contributions
HL proposed the idea of counting maize tassels via local counts regression, implemented the technical pipeline, conducted the experiments, analysed the results, and drafted the manuscript. ZG, YX and CS cosupervised the study and contributed in writing the manuscript. BZ helped to design the experiments and provided technical support and computational resources for efficient model training. All authors read and approved the final manuscript.
Acknowledgements
The authors would like to thank XiuShen Wei for useful discussion.
Competing interests
The authors declare that they have no competing interests.
Availability of data and materials
The MTC dataset and other supporting materials are available online at: https://sites.google.com/site/poppinace/.
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Funding
This work was supported in part by the Special Scientific Research Fund of Meteorological Public Welfare Profession of China under Grant GYHY200906033 and in part by the National Natural Science Foundation of China under Grant 61502187.
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Lu, H., Cao, Z., Xiao, Y. et al. TasselNet: counting maize tassels in the wild via local counts regression network. Plant Methods 13, 79 (2017). https://doi.org/10.1186/s1300701702240
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DOI: https://doi.org/10.1186/s1300701702240