The elastic modulus for maize stems
© The Author(s) 2018
Received: 3 October 2017
Accepted: 1 February 2018
Published: 8 February 2018
Stalk lodging is a serious challenge in the production of maize and sorghum. A comprehensive understanding of lodging will likely require accurate characterizations of the mechanical properties of such plants. One of the most important mechanical properties for structural analysis of bending is the modulus of elasticity. The purpose of this study was to measure the modulus of elasticity of dry, mature maize rind tissues using three different loading modes (bending, compression and tensile), and to determine the accuracy and reliability of each test method.
The three testing modes produced comparable elastic modulus values. For the sample in this study, modulus values ranged between 6 and 16 GPa. All three testing modes exhibited relatively favorable repeatability (i.e. test-to-test variation of < 5%). Modulus values of internodal specimens were significantly higher than specimens consisting of both nodal and internodal tissues, indicating spatial variation in the modulus of elasticity between the nodal and internodal regions.
Bending tests were found to be the least labor intensive method and also demonstrated the best test-to-test repeatability. This test provides a single aggregate stiffness value for an entire stalk. Compression tests were able to determine more localized (i.e., spatially dependent) modulus of elasticity values, but required additional sample preparation and test time. Finally, tensile tests provided the most focused measurements of the modulus of elasticity, but required the longest sample preparation time.
Maize is one of the world’s top crops and is used in hundreds of applications worldwide. But annual losses due to late season stalk lodging of maize is a serious challenge that hinders economic effectiveness of maize production. In particular, the problem of late-season stalk lodging (breakage of the stalk prior to harvest but after reproductive stage six) has been estimated to reduce worldwide corn yields by 5–20% [1, 2]. The complete characterization of structural and material properties of maize stems should enable a more comprehensive understanding of stalk lodging . However, a prerequisite to this approach is to first develop and validate accurate methods of measuring the mechanical tissue properties of maize stalks. Of the many tissue properties that can be measured, the longitudinal modulus of elasticity is perhaps one of the most important for structural bending analyses .
Determining the elastic modulus of the rind tissue of maize stems is challenging for several reasons. First, the irregular shape of maize stalks makes the specimens difficult to handle with standard mechanical test equipment. Second, the dependency of elastic properties of plant tissues on their state of maturation and moisture content has a strong effect on measured properties . Systemic testing, thus, would require control of maturity and moisture content during, preparation, storage, and testing. Third, the variability of biologic material requires testing of several specimens in order to statistically characterize untested parameters dictated by nature and not controlled in engineering environments. Thus, results from mechanical testing of biologic materials have an element of error that is not often quantified. Finally, maize stems consist of a complex structure whose various components are intimately connected. Thus, often only bulk material properties can be easily measured.
Due to the complex structure of maize stalks as well as the above-mentioned testing challenges, biomechanical data on the material properties of maize stalk tissues are currently limited to a handful of studies. These have included the determination of the compressive modulus of elasticity using dry internodal specimens with a length-to-diameter ration of 1:1 , bending modulus of elasticity of moist maize internodal specimens sampled at the pre-tasselling and dough growth stages [12, 13], the tensile modulus of elasticity of moist and dry rind tissue of maize stalks , and a reduced modulus of elasticity (indentation modulus) of dry rind fibers using nanoindentation [15, 16]. Most of these studies utilized only a single test method, and the reported values for rind modulus varied between 0.26 and 20 Gpa.
The goal of the present study was to obtain modulus of elasticity values of maize rind tissue by using a variety of mechanical loading modes to enable comparisons between these methods. Since maize is susceptible to late-season stalk lodging, dry, mature maize stalks were utilized in this study. Three loading modes were evaluated: transverse 3-point bending, longitudinal compression, and longitudinal tensile loading. From these tests, a total of 5 elastic modulus values were obtained. Direct comparisons between the values obtained by each method were used to determine the accuracy and reliability of each method, as well as the advantages and disadvantages of each testing method.
Sample sizes for each test type varied in proportion to the time required for test preparation and testing. A set of 1000 stalks was subjected to bending tests, a subset of 100 stalks were then tested in compression, and 20 thin tissue strips from 10 of the compression samples were selected for tensile testing. The following sections describe the stalk samples, specimen preparation, testing equipment, and the testing procedures that were used in this study.
Quantifying geometric properties of stalks
X-ray computed tomography (CT) was used to quantify geometrical properties. Stalks were scanned using an X5000 scanner (NorthStar Imaging, Rogers, MN, USA). The scanning process produced 2D cross-sectional images of the maize stalks. A customized computer program was used to extract cross-sectional areas of the rind and pith regions as well as the area moment of inertia from the CT data. The Cross-sectional area and the area moment of inertia were obtained in the constant geometry region of the internode directly above the loaded node in the three-point bending test. The scanning and morphology extraction are described in more detail in previous studies [17, 18]. It should be noted that using only a single value for the area or the moment of inertia of the stalk introduces some error in the estimation of the elastic modulus (i.e., stalks are tapered structures and both the diameter and moment of inertia changes along their length). However, for bending tests, this error has been shown to be negligible .
General conditions: length and aspect ratio of test specimens
Aspect ratio (length/width)
Bending (n = 1000)
950.84 ± 150.55
46.36 ± 9.26a
Compression (n = 100)
186.82 ± 26.11
9.64 ± 1.59a
Tensile (n = 20)
80.26 ± 1.67
25.81 ± 4.34b
Mechanical testing equipment
All tests were performed in a laboratory setting using an Instron universal testing machine (Model 5965, Instron Corp., Norwood, MA). Instrumentation control and data acquisition were managed with Instron software (Bluehill 3.0). All tests were displacement controlled, with force and displacement measured synchronously during the test. Low levels of deformation were applied in each test to avoid physical damage to test specimens. Maximum deformation and strain values of each test type are given below.
3-Point bending test
As shown in Fig. 2, maize stalks were supported at the initial and terminal nodes of each stalk, with a load applied at the node closest to the center of the stalk. It should be noted that this approach often dictates a non-symmetric loading scenario which is properly accounted for in Eq. 1. Stalks consisted of between 4 and 7 internode segments. A 500-N load cell was used to collect the force data at a frequency of 10 Hz, and stalks were deflected < 6 mm at a constant rate of 1.666 mm/s. This procedure ensured the test didn’t induce any permanent damage to the stalk. Additional details about the bending test methodology can be found in previous reports [20, 21].
In this equation, (L) is the distance between the left and right supporting anvils, (a) and (b) are the distances from the left and right anvils to the point of the applied load, respectively, and (I) is the area moment of inertia of the rind tissue. The structural contribution of the pith tissue is ignored in this approach. Previous reports indicate that this assumption does not introduce significant errors [10, 17, 20, 21], and it should be noted that the calculation of the rind modulus cannot be accomplished from a single test without this assumption.
Longitudinal compression tests were performed on a subset of 100 internodal specimens (Fig. 2). The internodal specimens used were prepared from stalks picked randomly from the 1000 stalk set used for bending testing. The internodal specimens were cut just below and above each node . Compression tests were conducted using two self-aligning platens (Cat No: S5722A, Instron Corp., Norwood, MA, USA), an extensometer with a reference length of 50 mm (Instron 2630 Series Dynamic Extensometer, Instron Corp., Norwood, MA, USA), and a 5-KN Instron load cell. The displacement rate was 0.1 mm/s and the sampling frequency was 33 Hz. Slope measurements were obtained by inducing < 0.35 mm of specimen deflection (strain values < 0.5%). This procedure ensured the tests did not induce any permanent damage to the specimens.
As previously reported  two estimates of elastic modulus can be obtained in a compression test of this nature; overall and local. Overall moduli were calculated using strains based on the displacement of the uppermost compression platen and the entire length of the specimen. Local moduli were calculated using the deformation recorded between the two arms of the extensometer. The overall compressive modulus represents a single aggregate value for all rind tissue in the internodal specimen and can be obtained relatively quickly since the extensometer is not required. The local compressive modulus provides a more focused measurement because it measures deformation within a limited region of the specimen. However, accurately measuring the local modulus generally requires conducting multiple axial tests. Further details on the local and overall modulus methodology are available in a previous paper .
Technical standards govern the processes for evaluating mechanical parameters of various synthetic materials as well as lumber. But few such standards exist for testing biological tissues such as corn stalks. Nevertheless, existing standards can provide useful guidelines for the measurement of such tissues. For example, ASTM D4761-13 recommends that tensile specimens be constructed with an aspect ratio (length/width) > 12 . This choice of aspect ratio helps minimize in influence of end effects. The aspect ratio of tensile specimens in this study was between 18 and 20.
Longitudinal tensile tests were performed on thin strips of rind tissue that were carefully dissected from compression-test specimens. The compression test specimens were first cut transversely with a rotary electric saw. Next, thin strips were cut lengthwise with a razor. The inner face and lateral sides of these thin strips were gently abraded to remove pith tissue and to improve geometric homogeneity of test strips. The length of prepared specimens ranged from 151.5 to 157 mm. The width of specimens ranged from 2.13 to 4.25 mm.
After the adhesive was fully cured, test specimens were mounted in the test fixture using side action fixed grips (Cat No: 2710-114, Instron Corp., Norwood, MA, USA). Serrated grip faces provided secure connection between the grips and the wood blocks. A specimen mounted for testing is shown in Fig. 3b.
A 5-KN load cell (Cat No: 2580-108, Instron Corp., Norwood, MA, USA) was used to collect the force data at a frequency of 33 Hz, and a deformation rate of 0.1 mm/s was used. The loads used in this test typically resulted in strain values < 0.5%. The displacement rate used is within range recommended for testing the tensile axial strength of lumber and wood-base structural materials .
Strain was calculated based on the distance between wood blocks and the displacement of the jaw fixtures. This approach was chosen to eliminate non-axial deformation which was induced when an extensometer was attached to the thin specimen. The validity of this approach was also confirmed experimentally: extensometer measurements were found to be virtually identical to those obtained from the displacement of the jaws.
When testing biological tissues, a preload and repeated application of load cycles is commonly used to bring the samples to a repeatable reference state . Therefore data was collected after applying an initial load of 10 N to each specimen. Five loading cycles were then applied, where in each cycle the load increased from 10 to 110 N and then returned to the 10 N initial state. Only measurements from the latter four loading cycles were employed in the tensile modulus calculation. This procedure did not induce any damage to the specimen. More information about the preloading and loading procedures can be found in .
Tensile elastic modulus is defined as the slopes of the corresponding stress–strain curve. The tensile stress, σ, was obtained by dividing the applied force (F) by the cross-sectional area of the rind strip (A r ). For each rind strip specimen, the cross-sectional area employed in the stress evaluation was estimated twice; using X-ray computed tomography and using a caliper. X-ray computed tomography areas were calculated using a customized computer program to estimate the cross sectional area of 2D X-ray images from the middle portion of test specimens. Cross-sectional areas obtained via calipers were accomplished by measuring the cross-sectional area of specimens at three distinct locations (i.e., from the top, middle, and bottom portions of each specimen). These three measurements were then averaged to obtain the cross-sectional area of the specimen. For small deformation such as those present in this study, strain can be obtained by dividing the change in length by the original length of the rind strip specimen prior to the 10 N pre-loading. Depending on the way cross-sectional areas were estimated, 2 tensile elastic moduli values were calculated and reported; Etensile and Etensile-caliper. The former obtained using X-ray images and the later using caliper measurements.
Repeatability and statistical analysis
When testing biologic materials, geometric and structural variations introduce an element of error that increases results uncertainty. Repeatability is one way to quantify uncertainty. Therefore, the repeatability of each test methodology used in this study was assessed. The design of this experiment consisted of 3 test types (bending, compression, and tension), 10 specimens per test type, and 5 repeated measurements per specimen. This structure resulted in 50 individual measurements for each method of determining the elastic modulus (bending, compression, and tension). The standard deviation was used to quantify test-to-test repeatability for each specimen following standard procedures .
Statistical analyses included unpaired and paired t tests. Unpaired tests were used to identify differences between testing methodologies, while paired tests were used to identify differences among the elastic moduli values obtained. For bending, compression, and tensile tests, the corresponding sample sizes were 1000, 100, and 20, respectively. Paired data (i.e., tests performed on specimens from the same stalk) was obtained for 10 stalks. Significance was established at p ≤ 0.05.
Repeatability statistics obtained from repeated tests of prepared specimens: 10 stalk specimens, 10 internodal specimens, and 10 tensile specimens
% Repeatability (Mean ± SD)
95% Successive tests confidence interval upper bound (%)
1.5 ± 0.9
3.8 ± 3.7
3.9 ± 2.7
1.9 ± 1.3
Linear stress–strain curves
Bending versus compression versus tensile elastic moduli comparison
The accuracy of tests such as these is difficult to establish because the exact value of the modulus of elasticity of each sample is unknown. In addition, biological tissues can be notoriously difficult to measure accurately. For example, a previous study used bending, compression, and tension test methods to compare the modulus of elasticity of barley and wheat stems . The study reported a modulus value of 7.3 GPa in tension and of 0.6 GPa in compression for the same wheat variety, values which correspond to percent discrepancies of (− 92%) and (+ 1117%), depending on which value is assumed to be correct.
However, all tests in this study produced modulus of elasticity values that were in the neighborhood of 12 GPa. For tests involving similar tissue types, the average discrepancy between median results was < 5% with a maximum discrepancy of 11%. The consistency of the tests in this study is one way to establish the accuracy of measured values.
Previous studies have reported different values for the modulus of elasticity of the rind tissue of dry maize. The lowest estimate in the literature was 0.26 GPa obtained using internodal specimen with a length-to-diameter ratio of 1:1 under compressive loads . The maximum estimate was 20 GPa obtained using dogbone shaped specimens in tension . In this study, straight-sided specimens were used for tensile testing as recommended when testing fiber reinforced materials having comparable dimensions .
Modulus of elasticity and tissue regions
Specimens in this study can be divided into two groups. Specimens in the first group consisted of both nodal and internodal tissues. Both bending tests and overall compression tests utilized nodal + internodal specimens. The second group of specimens consisted of internodal tissues only. Local compression tests and tensile tests utilized these specimens. As shown in Fig. 6, the measured value of elastic modulus tended to be different between these two groups. Previous studies have noted spatial variation in material properties, both between nodes [21, 28], and basal/apical variation between internodes . Because bending tests and overall compression tests utilize both nodal and internodal tissues, modulus of elasticity values obtained from these tests should be considered as aggregate values. On the other hand, local compression and tensile test methods utilize only internodal tissues, and therefore produce values that are likely more accurate than bending and overall compression tests. However, they are only valid for the tissue region from which they were taken.
The modulus of elasticity values of internode tissues were consistently higher than the values within the nodal + internodal tissues group. This suggests that internodal tissues have, on average, a higher modulus of elasticity than nodal tissues. This observation is consistent with the fact that internodal tissues are more organized and regular than nodal tissues [10, 21], and with previous work on nodal + internodal differences observed during bending tests .
Symmetry in elastic moduli
Although the elastic modulus is typically assumed to be symmetric (i.e., the same modulus value in both tension and compression), asymmetric moduli of plant tissues have been reported [11, 30, 31]. The specimens in this study consistently exhibited symmetry of the modulus of elasticity. This was evidenced in two ways. First, for nodal + internodal specimens, the modulus values obtained from bending tests (which involved both tensile and compressive strain) were similar to those obtained in compression, which involved only compressive strain. Second, for tests involving only internodal tissues, tensile and compressive tests yielded similar results. Future modeling studies can therefore utilize an assumption of symmetric moduli. Furthermore, modulus of elasticity values from any of these tests can be used (as appropriate) in such modeling studies. The symmetry obtained in this study supports the convection that measurements errors may be the cause of the asymmetry of the elastic moduli values reported in the previously mentioned studies.
Advantages and disadvantages of the various testing methods
Comparison of the different testing modes used to obtain the elastic modulus: testing time estimates, geometry assessment tool, and the repeatability variation
Preparation time min./specimen
Testing time min./specimen
Prep + Testing time min./specimen
Geometry assessment tool
% Repeatability mean (std)
~ 1 min
~ 2 min
~ 3 min
~ 3 min
~ 3 min
~ 6 min
~ 10 min
~ 40 min
~ 2 min
~ 42 min
The preceding discussion omitted the issue of geometric assessment. This issue is important because specimen geometry has a strong influence on the calculation of elastic modulus. In the current study, X-ray computed tomography (micro-CT) was used to assess test specimen geometry of most tests. The exception was the caliper-based tensile test (see Fig. 6). Micro-CT scanning requires expensive equipment and can be time consuming. On the other hand, it enables a highly detailed assessment of specimen geometry. The accurate assessment of specimen geometry is one reason that the repeatability values in this study were relatively low. The time associated with micro-CT scanning was excluded from Table 3 because it is highly dependent on the system being used. Alternatives to micro-CT scanning include measurement with hand tools such as digital calipers or obtaining cross-sectional images obtained with a flatbed scanner . Flatbed scanning would provide more consistent measurements, but would require more sample preparation and computer aided digital analysis. On the other hand, digital caliper measurements can be collected quite quickly, but are be significantly less reliable.
The data in this study were obtained using dry, mature maize stalks and tests were focused on assessing the modulus of elasticity of the rind tissue only. As such, the modulus of elasticity values reported in this study are relevant only to similar tissue and specimens. For bending and compression tests, the modulus of elasticity was calculated using equations that effectively neglect the structural influence of the pith tissue. Previous research by the authors indicates that this assumption effectively increases the calculated modulus of elasticity by about 3–5% . The data from those tests should therefore be viewed as lower bound estimates for the modulus of elasticity of dry, mature maize rind tissues.
Reliability estimates reported in this study involved repeated testing, but did not involve other sources of uncertainty. Other potential sources of uncertainty include sample preparation, the assessment of specimen geometry, the effect of shear, and the possible effect of indentation or section ovalization. Test-to-test variation due to these factors may be worth studying in the future. Deformation of the testing apparatus in this study was was negligible.
In addition to these factors, specimens typically exhibit slight variation in cross-sectional area in the axial direction. The reader should be aware that the reliability of any individual measurement is dependent upon all relevant sources of variation and uncertainty. In most cases, the overall reliability of these tests will be higher than the test reliability values reported in Table 2.
This study compared the modulus of elasticity values of the rind tissue of dry maize stalks obtained using different mechanical loading modes (bending, compression & tensile), and contrasted the accuracy and reliability of these same test. All methods produced modulus of elasticity values that were within the range 6–16 GPa, indicating that each of these methods is effectively measuring the same physical feature of the maize rind, and that each method is relatively accurate. All methods exhibited good test-to-test repeatability values.
Bending tests were found to be the fastest and most repeatable measurement approach. However, because the bending test requires a long slender specimen, it produces an aggregate estimate of the modulus of elasticity. Compression tests require somewhat more sample preparation and testing time, but provide modulus of elasticity values that are more localized. Finally, the tensile testing approach is the most intensive in terms of sample preparation, but produces the most localized measurement of the modulus of elasticity. This information can be used in creating structural models of maize stalk lodging, and to guide future experiments.
LA-Z, DR, and DC designed the research and wrote the manuscript. LA-Z, DR, and DC developed the experimental procedure of the approach. LA-Z, DR, and WS performed the experimental procedure. All authors read and approved the final manuscript.
We thank Monsanto Company, St. Louis, MO, USA for providing the maize stalk samples used in this study.
The authors declare that they have no competing interests.
Availability of data and materials
The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.
Consent for publication
Ethics approval and consent to participate
This study was supported by the National Science Foundation, Arlington, VA, USA (Grant # 1400973) and the U.S. Department of Agriculture, Washington, DC, USA (Grant # 2016-67012-24685). The funding agencies did not have any role in designing the study or in collecting, analyzing, interpreting the data or in writing the manuscript.
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