- Open Access
Leucine-rich-repeat-containing variable lymphocyte receptors as modules to target plant-expressed proteins
© The Author(s) 2017
- Received: 17 September 2016
- Accepted: 9 April 2017
- Published: 19 April 2017
The ability to target and manipulate protein-based cellular processes would accelerate plant research; yet, the technology to specifically and selectively target plant-expressed proteins is still in its infancy. Leucine-rich repeats (LRRs) are ubiquitously present protein domains involved in mediating protein–protein interactions. LRRs confer the binding specificity to the highly diverse variable lymphocyte receptor (VLR) antibodies (including VLRA, VLRB and VLRC types) that jawless vertebrates make as the functional equivalents of jawed vertebrate immunoglobulin-based antibodies.
In this study, VLRBs targeting an effector protein from a plant pathogen, HopM1, were developed by immunizing lampreys and using yeast surface display to select for high-affinity VLRBs. HopM1-specific VLRBs (VLRM1) were expressed in planta in the cytosol, the trans-Golgi network, and the apoplast. Expression of VLRM1 was higher when the protein localized to an oxidizing environment that would favor disulfide bridge formation (when VLRM1 was not localized to the cytoplasm), as disulfide bonds are necessary for proper VLR folding. VLRM1 specifically interacted in planta with HopM1 but not with an unrelated bacterial effector protein while HopM1 failed to interact with a non-specific VLRB.
In the future, VLRs may be used as flexible modules to bind proteins or carbohydrates of interest in planta, with broad possibilities for their use by binding directly to their targets and inhibiting their action, or by creating chimeric proteins with new specificities in which endogenous LRR domains are replaced by those present in VLRs.
- Protein targeting
- Leucine-rich repeat
- Variable lymphocyte receptor
In order to relay signals and interact with other molecules, proteins have acquired certain commonly used repetitive domains. One domain that has been shown to be involved in mediating protein–protein interactions is the leucine-rich repeat (LRR) domain, which is present in a variety of proteins from all domains of life including bacteria, eukaryotes, and even viruses . Each LRR domain contains a conserved segment with the consensus sequence LxxLxLxxN/CxL (where L, C, and N stand for leucine, cysteine, and asparagine, respectively, while x stands for any amino acid) and adopts the secondary structures of a β strand and an α helix connected by a loop . Multiple LRR domains arranged in tandem form a crescent-shaped structure, in which a continuous β-sheet on the concave side forms the most common surface for protein–protein interactions . The versatility of LRR domains in mediating protein–protein interactions is exemplified by the vast functions that proteins containing this domain may fulfill. For example, in plants, LRR domains are present in transmembrane receptor proteins of the LRR-receptor kinase (LRR-RK) and LRR-receptor protein (LRR-RP) classes [3–6], in intracellular nucleotide-binding site–LRR proteins (NBS–LRR) , in F-box proteins including hormone receptors of the E3 ubiquitin ligase class [8, 9], in a component of nucleocytoplasmic transport (RanGAP) , in intracellular proteins involved in pollen development (PIRLs) , and in extracellular cell wall LRR-extensins  and polygalacturonase-inhibiting proteins (PGIP) . The first three classes have radiated exponentially in plants and account for almost 3% of all genes in Arabidopsis [14, 15], and have been shown to be primarily involved in defense signaling and recognition, and plant development.
Variable lymphocyte receptors (VLRs) are non-self recognition receptors present in jawless vertebrates (Agnatha, which includes hagfishes and lampreys), involved in detecting invading microbes. They are the functional equivalent of immunoglobulin-based antigen receptors and antibodies in jawed vertebrates (Gnathostomata, which includes all other vertebrates from cartilaginous fish to mammals) [16–19]. Contrary to the immunoglobulin domains used by gnathostomes, VLR antibodies primarily bind to antigens using the concave surface formed by their LRR domains . VLR proteins have the following domains: a signal peptide, an N-terminal LRR (LRRNT), multiple LRRs with variable sequence (up to 10 have been observed in a mature VLR ; the first LRR and the last one are referred as LRR1 and LRRVe, respectively), an incomplete LRR (connecting peptide, LRRCP), a C-terminal LRR (LRRCT), and a flexible, invariant stalk followed by a transmembrane or glycosylphosphatidylinositol (GPI)-anchor region . Both the N- and C-terminal LRR domains have two characteristic disulfide bridges to stabilize the fold of the protein . Agnathans possess T-like and B-like lymphocytes in which each differentiated lymphocyte carries a unique set of variable LRR sequences in their mature VLR gene . The high variability in the LRR region of VLRs has been estimated to allow a potential repertoire of 1014–1017 VLR variants, a feat that is achieved by somatic diversification through the step-wise incorporation of different LRR donor sequences into the incomplete germline gene until an in-frame functional mature VLR is formed .
Three different VLRs exist in lampreys and hagfishes; VLRA, VLRB, and VLRC; with individual lymphocyte lineages only expressing a single functional VLR type [22, 24]. VLRA and VLRC are expressed by lymphocytes that resemble jawed vertebrate T cells. After antigen stimulation, these T-like lymphocytes proliferate and increase expression of proinflammatory cytokines, while their antigen receptors always remain attached to the cell surface [22, 25]. In contrast, VLRB-expressing lymphocytes differentiate into plasmablasts that secrete their VLRB receptors as disulfide-linked multimers that serve as the functional equivalent of jawed vertebrate antibodies [26, 27].
In this study, the feasibility of using LRR-containing lamprey-derived VLRBs to target in planta-expressed proteins was investigated. The VLRBs were shown to accumulate in different cellular compartments, and VLRBs that were targeted through the plant secretory pathway were indeed able to interact in planta with their target, HopM1, a bacterial effector protein from a plant pathogen. These results provide a proof-of-concept demonstration for engineering VLR-based protein-targeting LRR modules in planta.
Typically, only 0.1–0.5% of VLRBs in the immune-stimulated YSD library have sufficient affinity for detection of antigen binding by flow cytometry. Therefore, the YSD library is enriched for antigen-specific high-affinity VLRBs using one or two rounds of magnetic-activated cell sorting (MACS) with streptavidin-conjugated magnetic beads before using fluorescence-activated cell sorting (FACS) to specifically isolate the highest affinity clones. The FACS-sorted yeast cells are plated and individual yeast clones are tested for antigen binding. The nucleotide sequence of the VLRBs from high-affinity antigen-binding clones is determined and the VLRBs are cloned into plant expression vectors. Transient expression or stable transformants are then generated through Agrobacterium-mediated transformation of plants, after which the in planta binding of the VLRB to the antigen of interest and any phenotypes of interest can be evaluated.
Development of VLRBs against the bacterial effector HopM1
HopM1 is an effector from Pseudomonas syringae encoded in the conserved effector locus (CEL) . HopM1 is not only one of the most conserved effectors in P. syringae strains , but also its in planta localization and target are known [31, 32]. We decided to test the feasibility of using LRR-containing VLRBs in planta to target HopM1. The N-terminus of HopM1 (amino acids 1–300; HopM11–300) fused to an N-terminal hexahistidine tag was expressed and purified from Escherichia coli (Fig. 1). HopM11–300 was used instead of full-length HopM1 because of increased protein solubility and ease of purification. Purification was performed by using Ni–NTA agarose beads and ion-exchange chromatography. Purified N-terminal HopM1 was covalently conjugated to paraformaldehyde-fixed Jurkat T cells (as an adjuvant) and used to inject lamprey larvae to induce production of VLRB antibodies against HopM1 (VLRM1). Three lampreys were immunized a total of three times at 2-week intervals. After the final immunization, blood plasma was collected from the lampreys and tested for binding to HopM11–300 by ELISA. Plasma from lamprey-1 had the highest binding to HopM11–300 (at almost a 1 in a 1000 dilution of the plasma; Additional file 1: Figure S1), and as such, the VLRB repertoire from this lamprey was PCR amplified from total lymphocyte cDNA and used to construct a YSD library (of approximately 1.1 × 106 clones) to select for VLRM1 clones. The YSD library was enriched for clones with high-binding affinity for HopM1 by one round of MACS sorting using 100 nM of biotinylated HopM11–300, before FACS sorting for yeast cells expressing higher affinity VLRM1 clones were selected (Fig. 1).
In planta expression and visualization of VLRM1
In planta interaction between VLRM1 and HopM1
It is important to note that the immunoprecipitations did not use any reducing agents, as attempts to perform the immunoprecipitations with dithiothreitol in the buffer failed, probably because of the importance of the disulfide bonds for proper VLR folding. In the co-immunoprecipitation experiments, a protein band of approximately 100 kDa was observed in the western blot (Fig. 5d; the lithium dodecyl sulfate (LDS) buffer in which immunoprecipitated proteins were resuspended did not contain reducing agents either). The expected molecular weight of a dimer between VLRM1 and HopM11–300 is about 90–93 kDa (depending on the tags used), which is very close to the molecular weight of this specific band (protein multimers have been observed before even in denaturing SDS-PAGE conditions ), providing further support for the in planta interaction between these two proteins.
We have described an original method for targeting plant-expressed proteins using LRR-containing VLRBs (Fig. 1). After lamprey immunization with the protein of interest, yeast surface display is used to identify high-affinity VLRBs (Fig. 2b). Cloning the VLRB into a vector suitable for plant expression and Agrobacterium-mediated transformation of plants allows the targeting of specific proteins. In this study, we have successfully targeted the N-terminus of a bacterial effector from a plant pathogen, HopM1, by expressing an appropriate specific VLRB (VLRM1) in planta (Fig. 5d). VLRM1 interacted with HopM1 and not with an unrelated effector, and HopM1 failed to interact with a non-specific VLRB (VLRTLR5).
The different high-affinity sequences identified in this study for VLRM1 clones had very few polymorphisms. This lack of variability is not surprising, as only 3% nucleotide differences had been observed for 50 VLRA mRNAs (specific for hen egg lysozyme) , which are expressed by the lamprey T-like cells , but is in contrast with the finding that more than 30% of amino acids were different between seven VLRBs specific for the BclA Bacillus anthracis spore-coat protein . The anti-BclA VLRB clones were screened as multivalent, secreted proteins that bound with high avidity, even though the monomeric subunits had low affinity. In contrast, the HopM1- and hen egg lysozyme-specific VLRs were isolated using yeast display to select for the highest affinity clones, which are uncommon in the repertoire and therefore, have more limited sequence diversity. The β-strands in the concave region of VLRB, which, except for the leucines of the LRR, are highly variable in sequence and confer binding specificity , were clearly divergent in amino acid sequence when comparing VLRM1, VLRTLR5 and VLRHEL. The overall amino acid sequence identity between VLRM1 and VLRTLR5 was 68%, and between VLRM1 and VLRHEL was 77%, while the average amino acid identity between the variable amino acids (non-leucine) in the β-strands of LRR1, LRRRV, and LRRVe was only 20 and 19%, respectively.
VLRBs can be highly specific to the target being recognized, as VLRBs have been observed to differentiate between proteins that were 89% identical . VLRBs have been shown to bind not only proteins but also carbohydrates [20, 40], and as such, VLRBs could be used to target specific carbohydrate moieties in plants. If desired, the binding specificity of the VLRs may be improved in vitro by random mutation of the amino acids responsible for the interaction in their corresponding LRRs. A more than a 1000-fold increase in binding has been observed using this method for identifying VLRs that recognize hen egg lysozyme . In addition, VLRBs form high-avidity multimeric binding structures composed of 8–10 identical VLRBs as they are secreted . The cysteines necessary for forming these higher order multimeric structures are not present in VLRM1, as this truncated protein lacks the invariant stalk region containing the cysteines and as such, is unable to form these multimeric structures. Multimeric secreted VLRBs with potentially higher affinity could be produced in plants by adding the stalk region to the plant-expressed VLRs.
Cytoplasmic expression of VLRM1 was relatively low, especially when compared to secreted VLRM1 (Fig. 3a, b). Expression of cytoplasmic immunoglobulin antibodies in plants has also encountered the same problem, as even when the immunoglobulin gene is highly transcribed, the accumulation of cytoplasmic immunoglobulin proteins is barely detectable in plants (with a more than 300-fold difference in protein concentration being observed when comparing cytoplasmic and secreted antibodies) . Nanobodies®, the recombinant variable binding domain of heavy-chain only antibodies (VHH) from Camelids  offer another alternative to target plant proteins. However, high expression of nanobodies has only been observed in the apoplast  and chloroplasts . The low antibody accumulation in the cytoplasm probably reflects the inability of the antibodies to form disulfide bonds in the reducing conditions of the cytoplasm , and would explain the low expression observed in this study for cytoplasmic VLRM1. Some proteins can still form disulfide bonds in the cytosol, especially under oxidative stress conditions , so it is still possible for a fraction of the VLRM1 proteins to fold properly in the cytoplasm.
In contrast to cytoplasmic VLR, VLRM1 targeted to the apoplast or the TGN expressed well (Fig. 3a, c). HopM1 has been observed to be localized to the TGN when the effector was expressed in transgenic plants . In the future, plants with resistance against HopM1-expressing P. syringae strains could be developed by attaching SYP61 to VLRM1 and using VLRM1-ubiquitin ligase or NBS–VLRM1 fusions (see below). However, currently it is unknown if HopM1 localizes to the lumen of the TGN or to the surrounding cytoplasm. Based on this study, we predict that VLR-based binding with HopM1 or other TGN-targeted plant proteins would probably work only if these proteins are localized on the lumen side of the TGN, since disulfide bond formation is not as efficient in the cytoplasm.
Antibodies with immunoglobulin domains have been used in the past to target plant- or plant pathogen-expressed proteins. For example, immunoglobulins have been used to modulate in planta abscisic and gibberellic acid availability [48, 49]. Plant viruses have also been the target of antibodies, as targeting the coat protein, the RNA-dependent RNA polymerase, and the protease that cleaves the viral polyprotein precursor reduced in planta viral accumulation and symptom development [50–52]. Immunoglobulins against cell wall proteins of fungal pathogens have even been engineered to be linked to antifungal peptides, which ultimately lead to reduced symptom production in transgenic plants carrying the antibody fusion .
We anticipate several ways in which the LRR modules from VLRBs could be used for targeting proteins and/or modifying protein function in plants (Additional file 1: Figure S4). Firstly, since LRRs are used as modules for interaction in numerous plant proteins [14, 15], VLRBs could replace the binding domains of these proteins to generate chimeric VLRB-proteins with new binding specificities. The potential for using VLR technology is such that one can conceive creating plants with a pseudo-adaptive immune system, in which pattern-recognition receptors (PRR) and disease resistance proteins with new specificities against invading pathogens may be tailored as needed. VLRBs could replace the binding modules of receptor-like proteins and receptor-like kinases and the LRR domains of NBS–LRR proteins. Functional chimeric PRRs in which the LRR domains were swapped with those from a different PRR have already been characterized . Chimeric proteins responding to new stimuli and causing developmental changes could also be created (e.g., VLR–BRI1 chimera, BRI1 is the brassinosteroid hormone receptor) . So far, an unsuccessful attempt at constructing a functional PRR–VLR chimera, in which no plant responses against lysozyme (the antigen recognized by the VLRA) were observed, has been described . The newly characterized structure of the PRR bound to its ligand  might help in the future in constructing a proper functional chimera.
Antigen-specific VLRBs could also be used to explore a phenotype of interest by inhibiting the activity of a protein by direct binding (as has been observed for enzyme inhibitors carrying LRR domains) [2, 13] or by targeting a protein for degradation and observing the change in the phenotype. Direct inhibition would require a VLRB with a much higher affinity than that of the enzyme for its substrate. Since VLRBs with binding affinities in the picomolar range have been observed , this would be theoretically possible. For targeting proteins for degradation, VLRBs may be incorporated into LRR-containing E3 ubiquitin ligases that target proteins for proteasomal degradation in the plant cell. Multiple possibilities exist for the future use of LRR-containing VLRs for targeting plant-expressed proteins.
In this study, we have developed an original methodology for in planta targeting of proteins. This is achieved by immunizing lampreys with our target of interest, selecting VLRs with high-affinity for this protein target using flow cytometry and yeast surface display, and finally, expressing the target-specific VLR in planta. We found that VLR accumulation was higher when directed to the secretory pathway, although fusing the VLR to certain proteins, e.g., SYP61, might help stabilize them. With few systems available for in planta protein targeting, the VLR-based methodology offers the opportunity to bind and inhibit the function of specific plant proteins, and to construct chimeric proteins with new specificities in which the endogenous interacting domains are replaced by those of VLRs. This ultimately might facilitate the exploration and discovery of new phenotypes and mechanisms in plant biology.
Strains and antibiotics
Agrobacterium tumefaciens and E. coli strains (Additional file 2: Table S1) were grown on LB (Lennox) medium at 28–30 and 37 °C, respectively. Antibiotics were used at the following concentrations: 10 µg mL−1 gentamycin, 50 µg mL−1 kanamycin, 100 µg mL−1 rifampicin, and 50 µg mL−1 spectinomycin.
Saccharomyces cerevisiae strains (Additional file 2: Table S1) were grown on YPD (yeast extract peptone dextrose), SD-CAA (synthetic dextrose supplemented with casamino acids; 20 g L−1 dextrose, 6.7 g L−1 yeast nitrogen base, 100 mM sodium phosphate buffer, pH 6.0, and 5 g L−1 acid-hydrolyzed casamino acids lacking tryptophan) or SG-CAA (synthetic galactose supplemented with casamino acids; similar to SD-CAA but dextrose concentration is reduced to 1 g L−1 and 19 g L−1 galactose is included) media at 28–30 °C.
Plant growth conditions
Nicotiana benthamiana plants were grown at 22–24 °C with a 12-h photoperiod. Arabidopsis thaliana plants were grown under a 12-h photoperiod, at 23 °C when the lights were on and at 21 °C when the lights were off.
Sea lamprey culture
Sea lamprey larvae (Petromyzon marinus) of 12–15 cm in length were captured by commercial fishermen (Lamprey Services, Ludington, MI) and maintained in sand-lined, aerated aquariums at 16–20 °C. Lampreys were fed with brewer’s yeast. All lamprey experiments were approved by the Emory Institutional animal care and use committee (IACUC).
Expression and purification of the N-terminus of HopM1
Escherichia coli BL21(DE3) pET28::His 6 -hopM1 1–900 strain was grown at 37 °C until the O.D.600 of a 200-mL culture reached 0.5. Protein expression was induced with the addition of 0.1 mM isopropyl β-d-1-thiogalactopyranoside (IPTG) and the culture was grown for 6 h at 22 °C. Cells were lysed by sonication (using the VirSonic 600 ultrasonic homogenizer from VirTis), centrifuged, and the supernatant was incubated with Ni–NTA agarose resin (QIAGEN) to capture polyhistidine-tagged proteins. Proteins were eluted from the resin with 0.5 M imidazole, and the sample diluted with 3 volumes of 30 mM Tris–HCl pH 8.3. A second-step purification of HopM11–300 used the UNO S-1 ion exchange chromatographic column (Bio-Rad) and the BioLogic DuoFlow™ chromatography system (Bio-Rad). HopM11–300 was eluted from the ion exchange column with 433 mM NaCl, and then desalted and resuspended in phosphate-buffered saline (PBS), pH 7.6, by dialysis.
Lampreys respond to particulate antigens, such as intact viruses, bacteria and mammalian cells, but soluble proteins are weakly immunogenic on their own. Several adjuvants have been developed for vertebrates to enhance the immune response, most of which are ineffective in lampreys. Although complete Freund’s adjuvant can enhance the VLRB response, in our hands, it is toxic to lamprey larvae resulting in a high mortality rate. Given that mammalian cells are immunogenic, we determined that protein antigens or haptenated proteins covalently coupled to human Jurkat T cells by amine linkage reproducibly induced VLRB responses to both protein and hapten epitopes without toxicity. Accordingly, HopM11–300 was conjugated to Jurkat T cells before lamprey immunization.
For HopM11–300 conjugation, 108 Jurkat T cells were fixed overnight in 4% paraformaldehyde. The fixed Jurkat T cells were washed in 20 mM MES, pH 5.5, and then activated for amine conjugation with EDC/NHS (1-ethyl-3-(-3-dimethylaminopropyl) carbodiimide hydrochloride/N-hydroxysuccinimide) for 20 min at room temperature. Cells were briefly washed in PBS, and then 0.2 mg of HopM11–300 was added to the pelleted EDC/NHS-activated cells for 3 h at room temperature. After conjugation, HopM11–300-conjugated cells were washed once with PBS containing 10 mM Tris–HCl, pH 7.5; and stored at 4 °C until needed for lamprey immunization.
Sea lamprey larvae were sedated with 0.1 g L−1 of tricainemethanesulfonate (Tricaine-S; Western Chemical, Inc.) before injection into the coelomic cavity with 20 µg of recombinant HopM11–300 covalently conjugated to formaldehyde-fixed Jurkat T cells. Three lampreys were immunized for a total of 3 times at 2-week intervals. Two weeks after the final immunization, the lampreys were euthanized with 1 g L−1 of tricainemethanesulfonate and exsanguinated by tail severing. Blood was collected in a 30 mM EDTA solution (serving as an anticoagulant), and plasma and leukocytes were separated using a 55% Percoll gradient. The plasma samples were used to measure the lamprey VLRB response to immunization by ELISA, while the leukocytes were stored in RNAlater ® (Thermo Fisher Scientific) at −80 °C until needed for VLRB cDNA library cloning.
Enzyme-linked immunosorbent assay (ELISA)
ELISA plates coated with 5 µg mL−1 of recombinant HopM11–300 were blocked with 2% skim milk in TBST (20 mM Tris–HCl, 150 mM NaCl, and 0.1% Tween-20, pH 7.5), before incubation with serial dilutions of plasma from HopM1-immunized lampreys or control non-immunized plasma. VLRB binding was detected with an α-VLRB mouse monoclonal antibody (4C4)  and an alkaline phosphatase (AP)-conjugated goat α-mouse IgG polyclonal antibody (SouthernBiotech; this secondary antibody binds to the α-VLRB antibody). In between each incubation period, five washes with TBST were performed. Enzyme activity was detected after addition of an AP substrate (p-nitrophenyl phosphate, SIGMA-Aldrich), after which plates were incubated for 30 min at room temperature, followed by AP enzyme inactivation with 0.1 M NaOH. Absorbance readings at 405 nm were collected and the data was graphed using GraphPad PRISM software.
VLRB library construction
RNA was isolated from total leukocytes samples collected from lampreys immunized with HopM11–300 using the RNeasy kit (QIAGEN). RNA was reverse transcribed into cDNA using SuperScript® III reverse transcriptase (Invitrogen™) and oligo-dT primers. VLRB transcripts were amplified from the leukocyte cDNA by nested PCR using high-fidelity DNA polymerase (Novagen®). The first round of PCR used primers that annealed to the 5′ and 3′ untranslated regions of VLRB, AVL001 and AVL002 (Additional file 2: Table S2), respectively. The second round of PCR used primers that amplified only the VLRB antigen-binding domain, from the N-terminal LRR to the C-terminal LRR (primers AVL003 and AVL004, respectively). These primers had approximately 50 bp of sequence homology to the yeast surface display (YSD) vector (pCT-ESO) for cloning by in vivo homologous recombination in transfected yeast cells.
The pCT-ESO plasmid adds a c-Myc epitope at the end of the VLRB insert and anchors the VLRB to the yeast cell wall by fusing the protein to Aga2p. VLRB expression in this system is controlled under a galactose-inducible promoter. To clone the VLRB cDNAs, the BDNF gene from the pCT-ESO-BDNF plasmid  was removed by restriction digestion with NheI and BamHI, and NcoI digestion (New England BioLabs® Inc.) to eliminate the BDNF insert.
For VLR library transformation, tryptophan-auxotroph S. cerevisiae strain EBY100 was grown to the log phase in YPD media at 30 °C until the O.D.600 reached 1.0. Cells were washed with H2O, and incubated in 10 mM Tris–HCl, 10 mM DTT, 100 mM lithium acetate, pH 7.6, at 225 rpm and 30 °C for 20 min. After incubation, yeast cells were washed with H2O and resuspended in 1 M sorbitol to a concentration of 109 cell mL−1. Two hundred µL of yeast cells, 1 µg of digested pCT-ESO vector and 2 µg of the purified VLRB PCR product were mixed and electroporated at 2.5 kV using a Micropulser™ electroporator (Bio-Rad). The total number of transformants was estimated to be 1.1 × 106 VLRB clones. Aliquots of the transformed yeast library were stored at −80 °C in 15% glycerol.
Yeast surface display
Two rounds of enrichment for HopM11–300-binding VLRBs using Fluorescence-activated and Magnetic-activated cell sorting (FACS and MACS, respectively) were performed. For HopM11–300 biotinylation, the EZ-link NHS-LC-LC-biotin kit (Thermo Fisher Scientific) was used.
For determination of binding to HopM1 of individual yeast colonies, an overnight yeast culture was diluted into fresh SD-CAA medium and grown at 30 °C for 3 h. Culture was centrifuged and resuspended in SG-CAA medium (to induce VLRB expression) and further grown for 48 h at 20 °C. Fifty µL of yeast cells were washed once in staining buffer (PBS pH 7.4 with 1% BSA) and then incubated for 30 min with 50–250 nM biotinylated HopM11–300. Cells were washed thrice with staining buffer, and then incubated for 30 min at 4 °C in staining buffer with 5 µg mL−1 mouse α-Myc-Alexa Fluor® 488 (clone 4A6; EMD Millipore) and 2.7 µg mL−1 streptavidin, R-phycoerythrin conjugate (Invitrogen™). HopM11–300 binding was evaluated using the BD Accuri™ C6 flow cytometer (BD BioSciences) and a 488 nM excitation laser. For detection of Alexa Fluor® 488 fluorescence, a 533/30 nm filter (FL-1 channel) was used. For detection of phycoerythrin, a 585/40 nm filter (FL-2 channel) was used. The FL-1 channel was corrected by subtracting 5% of the FL-2 channel, while the FL-2 channel was corrected by subtracting 6.2% of the FL-1 channel.
To determine which events captured by the flow cytometer corresponded to VLRB binding of HopM1, the fluorescence intensity in the Alexa Fluor® 488 versus phycoerythrin plot was divided into four quadrants. The quadrant in the left lowermost corner represents those events in which neither VLRB expression nor HopM1 binding occurred, and its limits were determined by using samples in which VLRB expression was not induced. The quadrant in the right uppermost corner represents those events in which both VLRB expression and HopM1 binding occurred.
VLRBs were amplified from individual colonies of yeast surface display libraries using KOD hot start DNA polymerase (Novagen®) and Zymolase (Zymo Research), primers AVL005 and AVL006 (Additional file 2: Table S2), and a 30 min incubation at 37 °C prior to PCR (for the Zymolase to degrade the yeast cell wall). Purified PCR products were used as DNA templates with Phusion® high-fidelity DNA polymerase (Thermo Fisher Scientific) and primers AVL007 and AVL008 to clone into the pCR™8/GW/TOPO® entry vector (Invitrogen™). Prior to cloning, addition of adenine overhangs was performed using GoTaq® DNA polymerase (Promega).
The nucleotide sequence corresponding to the N-terminus of HopM1 (PSPTO_1375; amino acids 1–300) was amplified from P. syringae pv. tomato (Pst) DC3000 genomic DNA using Phusion® high-fidelity DNA polymerase (Thermo Fisher Scientific) and primers AVL009 and AVL010. The signal peptide sequence of AtPR1 (At2g14610; SP) was amplified from A. thaliana cDNA with primers AVL011 and AVL012. SYP61 (At1g28490) was amplified from A. thaliana cDNA with primers AVL013 and AVL014. mRFP1 was amplified from plasmid pGWB554  with primers AVL015 and AVL016. All these DNA sequences were cloned into pCR8™/GW/TOPO®. Pst DC3000 hopK1 (PSPTO_0044) was amplified with primers AVL017 and AVL018 from Pst DC3000 genomic DNA and cloned into pDONR207. The nucleotide sequence corresponding to the N-terminus of HopM1 was also amplified using primers AVL019 and AVL020, cloned into plasmid pGEM®-T-Easy (Promega Corporation) and then cloned into pET28a by using the restriction enzymes NdeI and EcoRI (New England BioLabs® Inc.).
To create a fusion between the signal peptide of AtPR1 (SP) and VLRM1, SP was amplified using primers AVL021 and AVL022 such that the amplicon had overhangs that were identical in sequence to the pCR8 vector on the 5′ end and VLRM1 on the 3′ end. The PCR product was purified and used in a second round of PCR with pCR8::VLR M1 ; both templates had overlapping sequences, so that after the second PCR a single plasmid containing the signal peptide fused to the VLR would be produced. After amplification, removal of the original template plasmid was performed using restriction enzyme DpnI (New England BioLabs® Inc.).
Overlap-extension PCR (OE-PCR) was used to create a fusion between SP and hopM1 using primers AVL011 and AVL023 to amplify SP with overlaps, and AVL024 and AVL010 to amplify hopM1 with overlaps. The purified PCR products were used on a second PCR with primers AVL011 and AVL010 to create SP-hopM1 1–900 , which was then cloned into pCR8™/GW/TOPO®. OE-PCR was also used to create fusions between SP and VLR TLR5 (using primers AVL011 and AVL022, and AVL025 and AVL008), and SYP61 and VLR M1 (using primers AVL007 and AVL026, and AVL027 and AVL014).
VLR M1 , VLR TLR5 , mRFP1, A. thaliana SYP61 and SP, Pst DC3000 hopM1 and hopK1, and fusion proteins were cloned into destination vectors pGWB514, pGWB517, and pGWB554 ; and pDest-35S-X-YFP-6xHis  using Gateway® recombination technology (Invitrogen™).
Alignment and 3-D structure modeling
Amino acid alignment was performed using MegAlign™ (DNASTAR®), and the alignment was graphed using BoxShade (Hofmann and Baron). Protein domains were predicted using the SMART tool .
3-D structure modeling was performed using SWISS-MODEL (Swiss Institute of Bioinformatics) and the structure of a VLRB specific for α-hen egg white lysozyme (VLRHEL; 3g3a) .
Transient in planta expression of VLRBs in Nicotiana benthamiana
Agrobacterium tumefaciens strains were grown overnight in LB with appropriate antibiotics, washed twice with 10 mM MgCl2, 10 mM MES (pH 5.6); and resuspended in the same buffer containing 200 µM acetosyringone to an O.D.600 of 0.2, except for the YFP culture, whose O.D.600 was adjusted between 0.010 and 0.025. Cultures were incubated in the dark for 3 h at room temperature, after which 5- to 6-week old N. benthamiana plants were infiltrated using a needleless syringe. Forty-eight hours post-infiltration, samples were collected for protein extraction or visualization on the microscope.
Stable expression of VLRBs in Arabidopsis thaliana
To generate transgenic A. thaliana plants, the floral dip method  was used. After seeds were collected, transformants were selected in ½ concentration Linsmaier and Skoog (LS) medium with 25 µg mL−1 hygromycin. Genomic DNA from putative transformants was extracted using the method of Edwards et al.  and the presence of the transgene confirmed by PCR using primers AVL007 and AVL014.
Frozen leaf tissue was ground using 3-mm zirconium oxide beads (Glen Mills Inc.) and the TissueLyser II homogenizer (QIAGEN) or, for larger quantities, using a mortar and a pestle. Ground tissue was incubated with 3 volumes (µL) of extraction buffer (0.5–1.0% Triton X-100, 150 mM NaCl, 100 mM Tris–HCl pH 7.5, 10 mM dithiothreitol [DTT], 5 mM EDTA, and protease inhibitor cocktail for plant cell and tissue extracts from SIGMA-Aldrich) per mg of tissue for 10 min at 4 °C, after which the sample was centrifuged to remove the tissue debris. Protein concentration was determined using the Bradford method (Bio-Rad protein assay), so that every sample within an experiment was adjusted to have the same concentration.
Electrophoresis and Western blotting
Polyacrylamide gel electrophoresis was performed using the NuPAGE® electrophoresis system (Thermo Fisher Scientific) and NuPAGE® Novex® 4–12% Bis–Tris gels following manufacturer’s recommendations (45 min at 200 V and 120 mA [maximum]). Protein transfer was confirmed by staining the PVDF membrane with Ponceau S stain (0.1% Ponceau S in 5% acetic acid). Western blotting was performed with the following antibodies: α-c-Myc and α-GFP (abcam®), α-HA-HRP (3F10; Roche), and α-rabbit IgG-HRP (Thermo Fisher Scientific). For chemiluminescent detection, the SuperSignal™ West Dura extended duration substrate (Thermo Fisher Scientific) and Blue Ultra Autoradiography film (GeneMate) were used.
Staining of gels during HopM11–300 purification was performed with Denville Blue™ protein stain (Denville Scientific Inc.) following manufacturer’s recommendations.
Proteins were extracted by incubating ground tissue in extraction buffer (0.5% Triton X-100, 150 mM NaCl, 50 mM Tris–HCl pH 7.5, 0.5 mM EDTA, and protease inhibitor cocktail for plant cell and tissue extracts from SIGMA-Aldrich) for 1 h at 4 °C. No reducing agent (e.g., DTT) was included in the extraction buffer. Total protein immunoprecipitated was adjusted to be the same for every sample within an experiment. Extracted proteins (diluted to have a Triton X-100 concentration of 0.2%) were incubated with 20 µL of GFP-nAb™ (Allele Biotechnology), α-c-Myc (SIGMA-Aldrich), or α-HA (clone HA-7, SIGMA-Aldrich) agarose beads for 1 h at 4 °C. Beads were centrifuged and washed 4 times, after which immunoprecipitated proteins were released from the beads by resuspending them in 75 µL of LDS buffer (Thermo Fisher Scientific) and incubating for 10 min at 70 °C.
Confocal and epifluorescent microscopy
Confocal images were taken with the Olympus FluoView™ FV1000 confocal microscope. For YFP detection, the excitation used a 515 nm argon gas laser (10 mW, at 10% intensity), while the emission was collected between 530 and 569 nm. For mRFP1 detection, the excitation used the 559 nm solid-state diode laser (10 mW, at 10% intensity), coupled with an emission collected between 570 and 600 nm. Images were visualized with a 40×-magnification oil-immersion objective that had a numerical aperture (NA) of 1.3. Images were acquired at a voltage (HV) lower than one that gave fluorescence signal for an untransformed control and at which very few pixels for the image were starting to saturate. In all images, the offset parameter was adjusted to 10% and the line Kalman integration to 3.
Epifluorescent images were acquired with the Olympus IX71 inverted microscope equipped with a 120-W metal halide lamp and a YFP filter (Semrock). The filter had an excitation of 500/24 nm and an emission of 542/27 nm. Images were visualized with a 10×-magnification objective and were acquired at an exposure time at which the untransformed control did not show any autofluorescence.
ACV designed and performed most of the experiments, and wrote the manuscript; KN performed experiments regarding HopM11–300 purification; MDC supervised experiments; BRH designed and performed experiments regarding lamprey immunizations and the yeast surface display library screens, and wrote the manuscript; and SYH designed and supervised the experiments, and wrote the manuscript. All authors read and approved the final manuscript.
We would like to thank Dr. Alexandre Brutus for suggesting the VLR project idea, Dr. Kyaw Aung for constructing the hopK1 constructs and for his help in confocal microscopy, Dr. John Scott-Craig for his help with protein purification, Dr. Melinda Frame for her help in confocal microscopy, James Kremer for his help using the flow cytometer, and Dr. Jian Yao for constructing the pEarleyGate104 vector without the Gateway cassette.
The authors declare that they have no competing interests.
Availability of data and material
All data generated or analyzed during this study are included in this published article and its supplementary information files.
Ethics approval and consent to participate
All lamprey experiments were approved by the Emory Institutional animal care and use committee (IACUC). Experiments involving plants complied with US institutional guidelines.
Funding was provided by the Gordon and Betty Moore Foundation (GBMF3037 [S.Y.H.]), the National Institutes of Health (Grant GM109928 [S.Y.H.] and AI072435 [M.D.C.]), and the US Department of Energy (the Chemical Sciences, Geosciences, and Biosciences Division, Office of Basic Energy Sciences, Office of Science; DE–FG02–91ER20021 for infrastructural support [S.Y.H.]).
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
- Kobe B, Kajava AV. The leucine-rich repeat as a protein recognition motif. Curr Opin Struct Biol. 2001;11:725–32.View ArticlePubMedGoogle Scholar
- Kobe B, Deisenhofer J. A structural basis of the interactions between leucine-rich repeats and protein ligands. Nature. 1995;374:183–6.View ArticlePubMedGoogle Scholar
- Jeong S, Trotochaud AE, Clark SE. The Arabidopsis CLAVATA2 gene encodes a receptor-like protein required for the stability of the CLAVATA1 receptor-like kinase. Plant Cell. 1999;11:1925–34.View ArticlePubMedPubMed CentralGoogle Scholar
- Stergiopoulos I, van den Burg HA, Okmen B, Beenen HG, van Liere S, Kema GH, et al. Tomato Cf resistance proteins mediate recognition of cognate homologous effectors from fungi pathogenic on dicots and monocots. Proc Natl Acad Sci USA. 2010;107:7610–5.View ArticlePubMedPubMed CentralGoogle Scholar
- She J, Han Z, Kim TW, Wang J, Cheng W, Chang J, et al. Structural insight into brassinosteroid perception by BRI1. Nature. 2011;474:472–6.View ArticlePubMedPubMed CentralGoogle Scholar
- Sun Y, Li L, Macho AP, Han Z, Hu Z, Zipfel C. Structural basis for flg22-induced activation of the Arabidopsis FLS2-BAK1 immune complex. Science. 2013;342:624–8.View ArticlePubMedGoogle Scholar
- Takken FL, Goverse A. How to build a pathogen detector: structural basis of NB-LRR function. Curr Opin Plant Biol. 2012;15:375–84.View ArticlePubMedGoogle Scholar
- Tan X, Calderon-Villalobos LI, Sharon M, Zheng C, Robinson CV, Estelle M, et al. Mechanism of auxin perception by the TIR1 ubiquitin ligase. Nature. 2007;446:640–5.View ArticlePubMedGoogle Scholar
- Sheard LB, Tan X, Mao H, Withers J, Ben-Nissan G, Hinds TR, et al. Jasmonate perception by inositol-phosphate-potentiated COI1-JAZ co-receptor. Nature. 2010;468:400–5.View ArticlePubMedPubMed CentralGoogle Scholar
- Boruc J, Griffis AH, Rodrigo-Peiris T, Zhou X, Tilford B, van Damme D, et al. GAP activity, but not subcellular targeting, is required for Arabidopsis RanGAP cellular and developmental functions. Plant Cell. 2015;27:1985–98.View ArticlePubMedPubMed CentralGoogle Scholar
- Forsthoefel NR, Dao TP, Vernon DM. PIRL1 and PIRL9, encoding members of a novel plant-specific family of leucine-rich repeat proteins, are essential for differentiation of microspores into pollen. Planta. 2009;232:1101–14.View ArticleGoogle Scholar
- Baumberger N, Ringli C, Keller B. The chimeric leucine-rich repeat/extensin cell wall protein LRX1 is required for root hair morphogenesis in Arabidopsis thaliana. Genes Dev. 2001;15:1128–39.View ArticlePubMedPubMed CentralGoogle Scholar
- Di Matteo A, Federici L, Mattei B, Salvi G, Johnson KA, Savino C, et al. The crystal structure of polygalacturonase-inhibiting protein (PGIP), a leucine-rich repeat protein involved in plant defense. Proc Natl Acad Sci USA. 2003;100:10124–8.View ArticlePubMedPubMed CentralGoogle Scholar
- Shiu S-H, Bleecker AB. Receptor-like kinases from Arabidopsis form a monophyletic gene family related to animal receptor kinases. Proc Natl Acad Sci USA. 2001;98:10763–8.View ArticlePubMedPubMed CentralGoogle Scholar
- Meyers BC, Kozik A, Griego A, Kuang H, Michelmore RW. Genome-wide analysis of NBS–LRR-encoding genes in Arabidopsis. Plant Cell. 2003;15:809–34.View ArticlePubMedPubMed CentralGoogle Scholar
- Velikovsky CA, Deng L, Tasumi S, Iyer LM, Kerzic MC, Aravind L, et al. Structure of a lamprey variable lymphocyte receptor in complex with a protein antigen. Nat Struct Mol Biol. 2009;16:725–30.View ArticlePubMedPubMed CentralGoogle Scholar
- Boehm T, McCurley N, Sutoh Y, Schorpp M, Kasahara M, Cooper MD. VLR-based adaptive immunity. Annu Rev Immunol. 2012;30:203–20.View ArticlePubMedPubMed CentralGoogle Scholar
- Kirchdoerfer RN, Herrin BR, Han BW, Turnbough CL Jr, Cooper MD, Wilson IA. Variable lymphocyte receptor recognition of the immunodominant glycoprotein of Bacillus anthracis spores. Structure. 2012;20:479–86.View ArticlePubMedPubMed CentralGoogle Scholar
- Luo M, Velikovsky CA, Yang X, Siddiqui MA, Hong X, Barchi JJ Jr, et al. Recognition of the Thomsen–Friedenreich pancarcinoma carbohydrate antigen by a lamprey variable lymphocyte receptor. J Biol Chem. 2013;288:23597–606.View ArticlePubMedPubMed CentralGoogle Scholar
- Han BW, Herrin BR, Cooper MD, Wilson IA. Antigen recognition by variable lymphocyte receptors. Science. 2008;321:1834–7.View ArticlePubMedPubMed CentralGoogle Scholar
- Pancer Z, Amemiya CT, Ehrhardt GR, Ceitlin J, Gartland GL, Cooper MD. Somatic diversification of variable lymphocyte receptors in the agnathan sea lamprey. Nature. 2004;430:174–80.View ArticlePubMedGoogle Scholar
- Hirano M, Guo P, McCurley N, Schorpp M, Das S, Boehm T, et al. Evolutionary implications of a third lymphocyte lineage in lampreys. Nature. 2013;501:435–8.View ArticlePubMedPubMed CentralGoogle Scholar
- Alder MN, Rogozin IB, Iyer LM, Glazko GV, Cooper MD, Pancer Z. Diversity and function of adaptive immune receptors in a jawless vertebrate. Science. 2005;310:1970–3.View ArticlePubMedGoogle Scholar
- Kasamatsu J, Sutoh Y, Fugo K, Otsuka N, Iwabuchi K, Kasahara M. Identification of a third variable lymphocyte receptor in the lamprey. Proc Natl Acad Sci USA. 2010;107:14304–8.View ArticlePubMedPubMed CentralGoogle Scholar
- Guo P, Hirano M, Herrin BR, Li J, Yu C, Sadlonova A, et al. Dual nature of the adaptive immune system in lampreys. Nature. 2009;459:796–801.View ArticlePubMedPubMed CentralGoogle Scholar
- Alder MN, Herrin BR, Sadlonova A, Stockard CR, Grizzle WE, Gartland LA, et al. Antibody responses of variable lymphocyte receptors in the lamprey. Nat Immunol. 2008;9:319–27.View ArticlePubMedGoogle Scholar
- Herrin BR, Alder MN, Roux KH, Sina C, Ehrhardt GR, Boydston JA, et al. Structure and specificity of lamprey monoclonal antibodies. Proc Natl Acad Sci USA. 2008;105:2040–5.View ArticlePubMedPubMed CentralGoogle Scholar
- Chao G, Lau WL, Hackel BJ, Sazinsky SL, Lippow SM, Wittrup KD. Isolating and engineering human antibodies using yeast surface display. Nat Protoc. 2006;1:755–68.View ArticlePubMedGoogle Scholar
- Alfano JR, Charkowski AO, Deng W-L, Badel JL, Petnicki-Ocwieja T, van Dijk K, et al. The Pseudomonas syringae Hrp pathogenicity island has a tripartite mosaic structure composed of a cluster of type III secretion genes bounded by exchangeable effector and conserved effector loci that contribute to parasitic fitness and pathogenicity in plants. Proc Natl Acad Sci USA. 2000;97:4856–61.View ArticlePubMedPubMed CentralGoogle Scholar
- Baltrus DA, Nishimura MT, Romanchuk A, Chang JH, Mukhtar MS, Cherkis K, et al. Dynamic evolution of pathogenicity revealed by sequencing and comparative genomics of 19 Pseudomonas syringae isolates. PLoS Pathog. 2011;7:e1002132.View ArticlePubMedPubMed CentralGoogle Scholar
- Nomura K, DebRoy S, Lee YH, Pumplin N, Jones J, He SY. A bacterial virulence protein suppresses host innate immunity to cause plant disease. Science. 2006;313:220–3.View ArticlePubMedGoogle Scholar
- Nomura K, Mecey C, Lee Y-N, Imboden LA, Chang JH, He SY. Effector-triggered immunity blocks pathogen degradation of an immunity-associated vesicle traffic regulator in Arabidopsis. Proc Natl Acad Sci USA. 2011;108:10774–9.View ArticlePubMedPubMed CentralGoogle Scholar
- Onda Y. Oxidative protein-folding systems in plant cells. Int J Cell Biol. 2013;2013:585431.View ArticlePubMedPubMed CentralGoogle Scholar
- Sanderfoot AA, Kovaleva V, Bassham DC, Raikhel NV. Interactions between syntaxins identify at least five SNARE complexes within the Golgi/prevacuolar system of the Arabidopsis cell. Mol Biol Cell. 2001;12:3733–43.View ArticlePubMedPubMed CentralGoogle Scholar
- Pedrazzini E. Tail-anchored proteins in plants. J Plant Biol. 2009;52:88–101.View ArticleGoogle Scholar
- Luo Y, Scholl S, Doering A, Zhang Y, Irani NG, Di Rubbo S, et al. V-ATPase activity in the TGN/EE is required for exocytosis and recycling in Arabidopsis. Nat Plants. 2015;1:15094.View ArticlePubMedPubMed CentralGoogle Scholar
- Stefano G, Renna L, Rossi M, Azzarello E, Pollastri S, Brandizzi F. AGD5 is a GTPase-activating protein at the trans-Golgi network. Plant J. 2010;64:790–9.View ArticlePubMedGoogle Scholar
- Gjetting KSK, Ytting CK, Schulz A, Fuglsang AT. Live imaging of intra- and extracellular pH in plants using pHusion, a novel genetically encoded biosensor. J Exp Bot. 2012;63:3207–18.View ArticlePubMedPubMed CentralGoogle Scholar
- Yoon SI, Kurnasov O, Natarajan V, Hong M, Gudkov AV, Osterman AL, et al. Structural basis of TLR5-flagellin recognition and signaling. Science. 2012;335:859–64.View ArticlePubMedPubMed CentralGoogle Scholar
- Gentile F, Amodeo P, Febbraio F, Picaro F, Motta A, Formisano S, et al. SDS-resistant active and thermostable dimers are obtained from the dissociation of homotetrameric beta-glycosidase from hyperthermophilic Sulfolobus solfataricus in SDS. Stabilizing role of the A-C intermonomeric interface. J Biol Chem. 2002;277:44050–60.View ArticlePubMedGoogle Scholar
- Tasumi S, Velikovsky CA, Xu G, Gai SA, Wittrup KD, Flajnik MF, et al. High-affinity lamprey VLRA and VLRB monoclonal antibodies. Proc Natl Acad Sci USA. 2009;106:12891–6.View ArticlePubMedPubMed CentralGoogle Scholar
- Zimmermann S, Schillberg S, Liao Y-C, Fisher R. Intracellular expression of TMV-specific single-chain Fv fragments leads to improved virus resistance in Nicotiana tabacum. Mol Breed. 1998;4:369–79.View ArticleGoogle Scholar
- Harmsen MM, De Haard HJ. Properties, production, and applications of camelid single-domain antibody fragments. Appl Microbiol Biotechnol. 2007;77:13–22.View ArticlePubMedPubMed CentralGoogle Scholar
- Teh YH, Kavanagh TA. High-level expression of Camelid nanobodies in Nicotiana benthamiana. Transgenic Res. 2010;19:575–86.View ArticlePubMedGoogle Scholar
- Jobling SA, Jarman C, Teh MM, Holmberg N, Blake C, Verhoeyen ME. Immunomodulation of enzyme function in plants by single-domain antibody fragments. Nat Biotechnol. 2003;21:77–80.View ArticlePubMedGoogle Scholar
- Biocca S, Ruberti F, Tafani M, Pierandrei-Amaldi P, Cattaneo A. Redox state of single chain Fv fragments targeted to the endoplasmic reticulum, cytosol and mitochondria. Biotechnology. 1995;13:1110–5.View ArticlePubMedGoogle Scholar
- Cumming RC, Andon NL, Haynes PA, Park M, Fischer WH, Schubert D. Protein disulfide bond formation in the cytoplasm during oxidative stress. J Biol Chem. 2004;279:21749–58.View ArticlePubMedGoogle Scholar
- Artsaenko O, Peisker M, zur Nieden U, Fiedler U, Weiler EW, Müntz K, et al. Expression of a single-chain Fv antibody against abscisic acid creates a wilty phenotype in transgenic tobacco. Plant J. 1995;8:745–50.View ArticlePubMedGoogle Scholar
- Shimada N, Suzuki Y, Nakajima M, Conrad U, Murofushi N, Yamaguchi I. Expression of a functional single-chain antibody against GA24/19 in transgenic tobacco. Biosci Biotechnol Biochem. 1999;63:779–83.View ArticlePubMedGoogle Scholar
- Tavladoraki P, Benvenuto E, Trinca S, De Martinis D, Cattaneo A, Galeffi P. Transgenic plants expressing a functional single-chain Fv antibody are specifically protected from virus attack. Nature. 1993;366:469–72.View ArticlePubMedGoogle Scholar
- Boonrod K, Galetzka D, Nagy PD, Conrad U, Krczal G. Single-chain antibodies against a plant viral RNA-dependent RNA polymerase confer virus resistance. Nat Biotechnol. 2004;22:856–62.View ArticlePubMedGoogle Scholar
- Gargouri-Bouzid R, Jaoua L, Rouis S, Saïdi MN, Bouaziz D, Ellouz R. PVY-resistant transgenic potato plants expressing an anti-NIa protein scFv antibody. Mol Biotechnol. 2006;33:133–40.View ArticlePubMedGoogle Scholar
- Peschen D, Li HP, Fischer R, Kreuzaler F, Liao YC. Fusion proteins comprising a Fusarium-specific antibody linked to antifungal peptides protect plants against a fungal pathogen. Nat Biotechnol. 2004;22:732–8.View ArticlePubMedGoogle Scholar
- Brutus A, Sicilia F, Macone A, Cervone F, De Lorenzo G. A domain swap approach reveals a role of the plant wall-associated kinase 1 (WAK1) as a receptor of oligogalacturonides. Proc Natl Acad Sci USA. 2010;107:9452–7.View ArticlePubMedPubMed CentralGoogle Scholar
- Koller T. Plant pattern-recognition receptor activation and exploration of techniques to engineer novel ligand specificities. Ph.D. dissertation, University of Wisconsin-Madison, Madison, Wisconsin, USA; 2014.Google Scholar
- Burns ML, Malott TM, Metcalf KJ, Hackel BJ, Chan JR, Shusta EV. Directed evolution of brain-derived neurotrophic factor for improved folding and expression in Saccharomyces cerevisiae. Appl Environ Microbiol. 2014;80:5732–42.View ArticlePubMedPubMed CentralGoogle Scholar
- Nakagawa T, Suzuki T, Murata S, Nakamura S, Hino T, Maeo K, et al. Improved Gateway binary vectors: high-performance vectors for creation of fusion constructs in transgenic analysis of plants. Biosci Biotechnol Biochem. 2007;71:2095–100.View ArticlePubMedGoogle Scholar
- Reumann S, Quan S, Aung K, Yang P, Manandhar-Shrestha K, Holbrook D, et al. In-depth proteome analysis of Arabidopsis leaf peroxisomes combined with in vivo subcellular targeting verification indicates novel metabolic and regulatory functions of peroxisomes. Plant Physiol. 2009;150:125–43.View ArticlePubMedPubMed CentralGoogle Scholar
- Letunic I, Doerks T, Bork P. SMART: recent updates, new developments and status in 2015. Nucleic Acids Res. 2015;43:D257–60.View ArticlePubMedGoogle Scholar
- Bent A. Arabidopsis thaliana floral dip transformation method. Methods Mol Biol. 2006;343:87–103.PubMedGoogle Scholar
- Edwards K, Johnstone C, Thompson C. A simple and rapid method for the preparation of plant genomic DNA for PCR analysis. Nucleic Acids Res. 1991;19:1349.View ArticlePubMedPubMed CentralGoogle Scholar