Fig. 4
Visualization of in planta VLRB protein expression. a Expression of intracellular YFP, HopM1-specific VLRB (VLRM1), and VLRM1 fused to A. thaliana syntaxin SYP61 (At1g28490) in Nicotiana benthamiana. Images were taken with the Olympus FluoView 1000 confocal microscope using a 515 nm laser for YFP excitation, while emission was collected between 530 and 569 nm. Fusing VLRM1 to SYP61 targets the VLRB to intracellular compartments, most likely the trans-Golgi network. b Expression of intracellular mRFP1, and predicted extracellular SP-mRFP1 and SP-VLRM1-mRFP1 in N. benthamiana. mRFP1 and HopM1-specific VLRB were targeted to the apoplast by fusing the VLRB to the signal peptide (SP) of Arabidopsis thaliana PR1 (At2g14610). Accumulation on the periphery of the cells was observed. Images were taken with the Olympus FluoView 1000 confocal microscope using a 559 nm laser for excitation and collecting the emission between 570 and 600 nm. White bar length represents 20 µm. Image brightness increased 40%