Table 2 Agrobacterium-mediated genetic transformation in Passiflora spp.

P. edulis f. edulis* P. edulis f. flavicarpa

Leaf disks

A. tumefaciens

GusA gene

0.67

pCAMBIA 1301 contains a hygromycin phosphotransferase (hpt) gene

[5]

Passiflora edulis f. flavicarpa Deg

Hypocotyls segment

Gene bombardment

pEPT8p35 and pBSAN236p35 genes

60%

CaMV35S (pEPT8)

[70]

P. edulis

Leave disks

A. tumefaciens

NIb and CP genes

pBluescript KS + vector

[71]

P. edulis

Leaf disks

A. tumefaciens

PWV CP gene

0.11–021

pCAMBIA 2300 binary vector

[72]

P. edulis var. flavicarpa

Leave disks

A. tumefaciens

CABMV-CP gene

0.19–0.0.67%

pCambia 2300 binary vector

[73]

P. alat

Hypocotyl

A. tumefaciens

pCABMV-dsCP-gene

0.89

[74]

P. cincinnata Mast.

Anther

A. tumefaciens

ß-Glucuronidase (uidA and nopaline synthase transcription terminator tNOS

57.89

pCAMBIA1304 (12,361 bp) binary vector

[76]

P. edulis

Wounded stem

A. tumefaciens

β-Glucuronidase (GUS), green fluorescent protein (GFP)

29%

pCAMBIA1301 and pCAMBIA1302

[78]

P. cincinnata and P. edulis f. flavicarpa

Root

A. rhizogenes

Chimaeric gene (nos-nptII-nos)

0.29

pRiA4b

[80]