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Fig. 3 | Plant Methods

Fig. 3

From: Establishment of an efficient cotton root protoplast isolation protocol suitable for single-cell RNA sequencing and transient gene expression analysis

Fig. 3

Optimization of the transient transformation system for cotton protoplasts. a–d Effects of Ca2+ concentration (a), transfection duration (b), plasmid concentration (c), and plasmid size (d) on transfection efficiency. e and f Bright field (e) and fluorescence microscopic (f) images of protoplasts transformed using pEASY-35S:GFP are shown. g and h Bright field (g) and fluorescence microscopic (h) images of protoplasts transformed using pKSE401-35S:GFP are shown

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Plant Methods

ISSN: 1746-4811