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Table 1 Illustration and description of the visual rating scale (VRS) used to evaluate the symptoms observed on the aerial parts of the seedlings

From: A new in vitro monitoring system reveals a specific influence of Arabidopsis nitrogen nutrition on its susceptibility to Alternaria brassicicola at the seedling stage

Illustration of a representative cotyledon/seedling

Visual rating description

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Rating = 0

No mycelium colonization nor necrosis on seedlings

No impact on seedling development, four-leaf stage seedlings in general

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Rating = 1

Minimal mycelium colonization and necrosis on seedlings (< 25% of surface area)

No impact on seedling development, four-leaf stage seedlings in general

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Rating = 2

Mycelium colonization and necrosis on seedlings

(25% < necrosis < 50% of surface area)

Small impact on seedling development, smaller four-leaf stage seedlings in general

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Rating = 3

Important mycelium colonization and necrosis on seedlings

(50% < necrosis < 75% of surface area)

Impact on seedling development, two-leaf stage seedlings in general

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Rating = 4

Almost total mycelium colonization and necrosis on seedlings

(75% < necrosis < 99% of surface area)

Important impact on seedling development, cotyledon stage seedlings in general

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Rating = 5

Total mycelium colonization and necrosis on seedlings

(= 100% of surface area)

Dead cotyledon stage seedlings

  1. The scale integrated colonization by the mycelium and necrotic area on cotyledon and hypocotyl criteria, and was based on a score ranging from 0 to 5 (no symptoms to 100%) assigned by an expert observer