Preparation of uniformly labelled 13C- and 15N-plants using customised growth chambers

Table 2 Adapted Hoagland solution

Salts	Hoagland Stock solutions [g/l]	Germination period [mg/l]	Growing period [mg/l]
KNO^a₃	202	101	404
Ca(NO₃)₂× 4H₂O^a	236	472	472
C₁₀H₁₂N₂NaFeO₈ (Ferric sodium–EDTA)	15	3.75	0.975
MgSO₄× 7H₂O	493	123.3	98.6
NH₄NO^a₃	80	8	8
H₃BO₃	2.86	0.143	0.072
MnCl₂× 4H₂O	1.18	0.59	1.18
ZnSO₄× 7H₂O	0.22	0.88	0.33
CuSO₄	0.051	0.051	0.051
Na₂MoO₄× 2H₂O	0.12	0.024	0.0072
KH₂PO₄	136	68	68

^aSalts substituted with highly ¹⁵N-enriched analogues in the ¹⁵N-labellling experiment

ISSN: 1746-4811