Fig. 2

Reference gene quantification cycle (Cq) distributions. Considerable variability in Cq value was observed for the eleven candidate reference genes assessed across the internode, leaf and inflorescence tissue samples. The whisker caps show the distribution of the highest and lowest Cq values. The boxes indicate the first and third quartile, while the middle line marks the median. The majority of the assessed candidate reference genes returned an average Cq value ranging between 21.9 and 25.3 cycles