Fig. 1
From: Real-time monitoring of PtaHMGB activity in poplar transactivation assays
Design and validation of pPtaLHY2::LUC transcriptional reporter in poplar. a PtaLHY2 mRNA expression profile in long day (LD) conditions obtained by qRT-PCR. Data indicates mean ± SD of three technical replicates. This experiment was performed twice. A representative result is shown in this figure. Upper bar indicates the photoperiod: black boxes represent the night and white boxes represent the day, respectively. b Phylogenomic analysis obtained by mVISTA plot using the LHY1 and LHY2 5′ upstream ATG sequences from Populus trichocarpa (Pt), Populus tremula × tremuloides (Ptt) and Salix purpurea (Sp). Populus tremula × alba was used as a reference sequence. Regions of 6 bp with more than 70% of identity are shadowed in pink. c pPtaLHY2::LUC activity detected by luminescence assay during 2 days under LD and 2 days under continuous light (LL) conditions. The activity of pPtaLHY2::LUC line #5 is shown in this figure. Data indicates mean of three independent assays. Error bars indicate SD of the mean. d Period versus relative amplitude error analysis for the three transgenic lines analyzed. Data indicate mean of three independent assays for every line. Error bars indicate SD of the mean