Table 3 Details of the settings for commonly used fluorescent signals
From: Protocol: a method to study the direct reprogramming of lateral root primordia to fertile shoots
Signal | Excitation wavelength (nm) | Emission wavelength range (nm) |
|---|---|---|
CFP | 458 | 465–515 |
GFP | 488 | 495–530 |
YFP/VENUS | 514 | 520–545 |
Propidium iodide | 514/561 | 585–630 |
FM4-64 | 514/561 | 620–750 |
Chlorophyll autofluorescence | 488/514/561 | 620–750 |