Processing of BSE images of A. thaliana leaf for analysis of cell size. A summary of the main steps in the procedure are shown here for an example image (provided as Additional file
). Not all the steps are shown here; a full description of the procedure and a macro are provided in Additional file 3. Scale bar = 30 μm in original image (1). Colours in step 6 ('Analyze particles’) indicate masks defining cell wall outlines (yellow) and cell areas (blue) in final processed image.