Critical point dried, uncoated A. thaliana tissues examined in the VP-SEM. (A) Mature rosette leaf showing overall leaf morphology and distribution of trichomes (t). (B) Higher magnification view of leaf showing trichome (t), bright cell wall outlines of pavement epidermal cells (arrowheads), and stomatal guard cells (s). (C) Developing seed (approx 7 days after flowering), and (D) higher magnification view showing 3D views of epidermal cell walls revealed by BSE imaging. Solid arrowheads = junction between epidermal outer periclinal and anticlinal cell walls, open arrowheads = junction between epidermal anticlinal walls and periclinal walls of epidermal and sub-epidermal cells, n = nucleus. Simultaneous capture and comparison of SE (E) and BSE (F) images of silique outer epidermis. White arrows show stomata charging in (E), black arrows in (F) show same stomata in the BSE image, arrowheads show cell outlines. Accelerating voltage 20 kV and chamber pressure 10 Pa (A-D) or 80 Pa (E, F). Scale bars = 20 μm (B, D), 50 μm (C), 100 μm (E, F) and 200 μm (A).