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Figure 6 | Plant Methods

Figure 6

From: Evaluation of alternative RNA labeling protocols for transcript profiling with Arabidopsis AGRONOMICS1 tiling arrays

Figure 6

Strand specificity of sample labeling. (A) Leaf RNA was labeled using random priming and hybridized to AGRONOMICS1 arrays. Sense and antisense expression signals were calculated from the same CEL files using alternative CDF files. (B) Leaf and flower RNA was labeled using random priming and hybridized to AGRONOMICS1 arrays, and sense expression signals were calculated. Lines in (A) and (B) indicate: signal identity (x = y), solid lines; fold change of +2 and −2, dashed lines; empirical threshold for background signals, dotted lines.

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