Skip to main content
Figure 1 | Plant Methods

Figure 1

From: Evaluation of alternative RNA labeling protocols for transcript profiling with Arabidopsis AGRONOMICS1 tiling arrays

Figure 1

Detection of antisense transcript signals on AGRONOMICS1 arrays. Bars represent gene annotations. Genes of interest are yellow, other genes grey. Probe signals from the plus strand are cyan and from the minus strand green. The upper two rows of signals show RNA from irradiated seedlings, the lower two rows RNA from dark-grown seedlings. (A) AT1G15950, a gene on the plus strand. Signals are highest for probes from annotated exons and on the plus strand. (B) AT1G31130, a gene on the minus strand. Signals are highest for probes on the minus strand. (C-D) AT5G64401 (C) and AT4G31875 (D) are genes on the minus strand. However, signals are highest on the plus strand strongly suggesting antisense transcription. Note that the signals are specific for irradiated seedlings.

Back to article page

Plant Methods

ISSN: 1746-4811