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Table 1 PDS silencing in B. distachyon

From: Investigations of barley stripe mosaic virus as a gene silencing vector in barley roots and in Brachypodium distachyon and oat

 

Symptoms

Plants with symptoms

Expt. 1a

S

5/12

PDS

V

6/12

Expt. 1b

S

4/10

PDS

V

5/10

Expt. 2

S

10/22

PDS

V

3/22

Expt. 2

S

0/22

GFP

V

16/22

Expt. 3

S

20/28

PDS

V

5/28

Expt. 3

S

0/28

GFP

V

23/28

  1. Virus symptoms and PDS silencing in B. distachyon leaves 3 weeks after inoculation with BSMV-BdPDS or BSMV-GFP375.
  2. S: plants displaying photobleaching (silencing) as well as virus symptoms. V: plants displaying virus symptoms only. Plants in experiment 1b were inoculated with BSMV-T4-BdPDS, otherwise BSMV-USER-BdPDS was used. Plants were inoculated at 11 (expt. 1), 14 (expt. 2), or 19 (expt. 3) days after sowing.
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Plant Methods

ISSN: 1746-4811