Detection of BSMV coat protein by ELISA. Barley (Black Hulless) and oat (Mellys, S75, Belinda) plants were left uninoculated or inoculated with BSMVCV42-α, -β and -γ, BSMVND18-α, -β and -γ or a combination of BSMVCV42-α, -β and BSMVND18-γ. Extracts of the third leaf, harvested at 14 dpi, were assayed by DAS-ELISA to detect BSMV coat protein. The average OD405 values of five plants from each group are shown. Error bars indicate standard deviations.