Figure 6
From: Isolation of a strong Arabidopsis guard cell promoter and its potential as a research tool
pGC1(D1)::anti-GFP caused reduction of GFP expression in guard cells of 35S::GFP plants. A. Leaf epidermis of a 35S::GFP transgenic plant (bright field with GFP filter). The arrows mark stomata. B. The fluorescence imaging of same leaf epidermis shown in A. Stomata are marked by yellow arrows. Note that both the guard cells and surrounding epidermal cells are fluorescent. C. Leaf epidermis of a T1 transgenic plant expressing pGC1(D1)::anti-GFP in the 35S::GFP background. All stomata are marked by yellow arrows. D. The fluorescence imaging of the same leaf epidermis shown in C. Note that 7 (marked by blue arrows) out of 8 stomata showed reduced GFP expression compared with the surrounding epidermal cells. One pair of guard cells (marked by the yellow arrow) still exhibited moderate GFP expression. This stomate was relatively immature compared with the other 7 stomata.