AFLPsim: an R package to simulate and detect dominant markers under selection in hybridizing populations
© Balao and García-Castaño; licensee BioMed Central. 2014
Received: 24 July 2014
Accepted: 24 November 2014
Published: 13 December 2014
In spite of a large diversity of approaches to investigate loci under selection from a population genetic perspective, very few programs have been specifically designed to date to test selection in hybrids using dominant markers. In addition, simulators of dominant markers are very scarce and they do not usually take into account hybridization.
Here, we present a new, multifunctional, R package for dominant genetic markers, AFLPsim. This package can simulate dominant markers in hybridizing populations and implements genome scan methods for detecting outlier dominant loci in hybrids. In addition, it includes tools for further manipulating the results, plotting them and other tasks. We describe and tabulate the major functions implemented in AFLPsim. In addition, we provide some demonstration of its use and we perform a comparative study with other software. Finally, we conclude by briefly describing the input and output formats.
The R package AFLPsim application provides several useful tools in the context of hybridization studies. It can simulate dominant markers in hybridizing populations and predict their demographic evolution. In addition, we implement a new genome scan method for detecting outlier dominant loci in hybrids, which shows a rather high sensitivity and is very conservative in comparison with Gagnaire et al.’s, Bayescan and introgress. The application is downloadable at http://cran.r-project.org/web/packages/AFLPsim/.
KeywordsDemographic simulation Dominant markers Genome scan Hybridization Outlier loci R package
The study of natural hybridization has been the focus of much attention in Evolutionary Biology [1, 2]. Hybridization has recently been perceived as a catalyst not only for speciation but also for major evolutionary innovations . Hybrid zones offer a “window on the evolutionary process” involving divergence at many loci by a balance between dispersal and selection . Cline theory has provided a conceptual framework to understand the forces maintaining hybrid zones and to help infer the relevant evolutionary parameters describing the introgression of traits across hybrid zones . However, in non-stable hybrid zones, selection in early generations has a central role in the establishment and fate of hybrids and progenitors [1, 4]. In these early generations, several hybrid categories can be easily distinguished (first generation hybrids –F1–, outcrosses between F1 individuals –F2–, or backcrosses to parental “A” –BxA– and backcrosses to parental “B” –BxB–, for instance). Hence, identifying markers under selection on these early-generated hybrids can provide tremendous knowledge about the stability of hybrid zones.
Although next generation sequencing (NGS) has transformed our ability to identify the genes underpinning selection/adaptation , a complementary approach with potentially neutral markers such as Amplified Fragment Length Polymorphisms (AFLPs) allows a cost-effective screening of the genomes of a large number of individuals . AFLP has been very successfully used in the identification of hybrids [8, 9] and outlier loci presumably under selection [10–12]. However, in spite of a large diversity of approaches to investigate loci under selection from a population genetic perspective [13–15], no program has been specifically designed to date to test selection in hybrids using dominant markers. Nevertheless, genomic clines have been used to identify molecular markers with patterns of introgression inconsistent with neutrality (e.g. introgress[16, 17]). However, detecting outliers in a hybridizing framework using dominant markers can be a real challenge. Long periods of time are needed to identify loci that have experienced a history of weak selection, as cumulative effect is necessary to produce a detectable signal in the DNA polymorphism of the underlying loci . Therefore, for hybrids in early generations, loci under weak selection would remain undetectable. In addition, dominance imposes difficulties to estimate allelic frequencies, especially when the frequency of the presence-allele is high . Scoring errors and a low sample size can also affect the correct estimate of the allelic frequencies. Furthermore, previous methods used to detect dominant loci under selection have shown a substantial proportion of false positives among the detected outliers . Hence, genome scan in hybrid zones should be capable to correctly estimate expected allelic frequencies under neutrality in hybrids overcoming these problems. Moreover, it should be sensitive enough to detect loci under moderate selection as well as keeping the false positive rate close to null.
Additionally, being able to simulate dominant markers in a hybrid zone is important to obtain a better knowledge about expected patterns of hybridization. When experimental treatments are not feasible, in-silico simulations have been widely used to test population genetic hypotheses . Furthermore, genetic simulations have been also used to understand the statistical efficiency of several genetic methods and to compare different approaches . Finally, simulations are of practical use when analysing data from a real system, as they can compare observed genetic distributions with the theoretically expected ones. In spite of its importance, simulators of dominant markers are very scarce  and they do not usually take into account hybridization, but see . Again, dominance of markers is one of the major problems in the simulation process, as allele frequencies have to be estimated from phenotypic data.
Here, we describe AFLPsim, a software package designed to overcome these limitations by implementing a dominant marker simulator of hybridization and two genome scan algorithms (Gagnaire et al.’s  and a new method called bal&gar-ca) specifically designed to detect outlier markers in recent generated hybrids (F1, F2, BxA and BxB). The software is written in a statistical, open source, scripting R language , and released under the GPL license to guarantee the continuing availability of the source code.
Functions of the AFLPsim package
conducts demographic analysis in hybrid populations.
generates multilocus dominant hybrids individuals from parental profiles.
generates multilocus dominant parental and hybrid individuals.
calls Bayescan 2.1 program  from R to a set of populations.
conducts genome scan on F1 and backcross individuals .
estimates the hybrid index calling the introgress package.
plots phenotypic frequencies of hybrids on a neutral hybridization model.
plots results of ‘demosimhybrid’ function.
converts simulation to the genind format .
writes the input file for Arlequin from the simulation results.
writes the input file for Bayescan from the simulation results.
writes the input file for Popgene from the simulation results.
converts simulation to the introgress format .
writes the input file for Newhybrids from the simulation results.
writes the input file for Structure from the simulation results.
Simulating hybridization and demographic evolution
and pB is the frequency of the presence-allele in the parental population B ().
Users can choose both the intensity of this coefficient (s) and the number of markers under selection.
where M is the assortative mating matrix.
Epifanio & Philipp’s model suffers from several pitfalls, e.g. migration is not taken into account and it is not spatially explicit [26, 28]. However, it has been successfully used to explain the extinction of progenitors in several hybrids zones [28, 29].
where is the observed frequency of band presence at one locus, is the number of F1 hybrids and is the expected frequency of band presence at that locus.
- (i)If it is within the two most extreme values, we conclude the fragment is not under selection (i.e. ).
- (ii)If not, we choose the closest of the four frequencies to be the expected value of the binomial test.
In both methods, the False Discovery Rate (FDR) correction is used to counteract for multiple comparisons and control for the expected proportion of the incorrectly rejected null hypotheses.
In addition, we also include a function that calls the Bayescan program  from R to perform a Bayesian estimation of selection; in this way, we facilitate the analysis and plotting of results of this efficient software. Moreover, the hybrid index (i.e. the genome-wide admixture) for the simulated hybrid individuals is calculated with the ‘hybridindex’ function. The maximum likelihood estimates (together with the 95% confidence intervals) of this hybrid index can be obtained with this function, which is a wrapper for the ‘est.h’ function of the package introgress.
Data manipulation and visualisation
AFLPsim functions do not require external input files out of the R environment (Table 1). However, for the simulation of hybrids from user-specified parental data, these should be loaded to R as a matrix or a data.frame. Simulation results can be readily used by multivariate and phylogenetic methods of other R packages (e.g. ade4; adegenet). Our package is also able to export Arlequin formatted data  and Popgene to estimate summary statistics (e.g. F-statistics, Shannon index or polymorphic loci) from the data set. Our package is also able to export data formatted for several popular population genetic computer programs such as Structure and Newhybrids. In addition, AFLPsim contains functions that produce graphics for visualising the expected frequencies under neutrality for loci under selection across the different hybrid classes. Finally, our package includes a function that plots the results of the demographic evolution model in a hybrid zone.
Results and discussion
To demonstrate the capacities of AFLPsim, we assessed some comparisons on the behaviour of our genome scan method vs. that of other software. We have also created several illustrative examples, which can be easily reproduced. An example of the application of AFLPsim for investigating introgression patterns (bal&gar-ca method) and demographic dynamics (Epifanio & Philipp’s model) in a hybrid zone can be found in .
Genome scan comparisons
We investigated the performance of our method (bal&gar-ca) under different scenarios using a simulation study and its efficiency was compared with that of Gagnaire et al.’s, Bayescan and introgress. Although Bayescan is not specifically designed for hybrids, we used it for comparison, as it is one of the most popular genome scan software. In this case, we used both parentals and F1 hybrids as three independent populations. introgress has been used to explore introgression between genomes through the genomic cline method. This method is able to identify those molecular markers with introgression patterns inconsistent with neutrality and, therefore, detecting possible loci under selection.
We performed three different simulation experiments to compare the outlier detection efficiency of different genome scan methods, different selection coefficients, different sample sizes and different percentage of selected loci in the genome. We carried out all the simulations on the University of Oslo Bioportal (http://www.bioportal.uio.no) using the ‘hybridsim’ function.
1) Comparison of different genome scan methods
We compared the results obtained with four methods (bal&gar-ca, Gagnaire et al.’s, introgress and Bayescan), with three selection coefficients (s = 2.162, log (s + 1) = 0.5, weak selection; s = 99, log (s + 1) = 2, strong selection; s = 999, log (s + 1) = 3, very strong selection). 100 F1 hybrid individuals were simulated with ‘hybridsim’ from two parental populations (A and B) of 100 individuals each one. We simulated 1000 independent loci (i.e. based on linkage disequilibrium), where 100 were introgressed under directional selection in the F1. Every scenario (i.e. every selection coefficient) was replicated 100 times. Genome scans were performed on simulations with our method (bal&gar-ca), that by Gagnaire et al., introgress and Bayescan 2.1, assessing the ability of each one for detecting selection of different intensities as well as the proportion of failures as false positives.
Summary of sensitivity (true positive rate ± SD) and type I error rate (false positive rate ± SD) for outlier methods tested with 100 simulated data for three regimes of divergent selection
Weak selection (s = 2.162)
Strong selection (s = 99)
Very strong selection (s = 999)
Type I error
Type I error
Type I error
0.124 ± 0.039
0.000 ± 0.000
0.479 ± 0.050
0.000 ± 0.000
0.487 ± 0.049
0.000 ± 0.000
Gagnaire et al.’s
0.572 ± 0.052
0.147 ± 0.012
0.621 ± 0.048
0. 149 ± 0.012
0.619 ± 0.049
0.147 ± 0.013
0.454 ± 0.034
0.127 ± 0.015
0.717 ± 0.061
0.126 ± 0.010
0.723 ± 0.052
0.128 ± 0.014
0.025 ± 0.021
0.029 ± 0.001
0.035 ± 0.019
0.012 ± 0.004
0.080 ± 0.019
0.037 ± 0.005
2) Impact of different scenarios on outlier detection
In the second approach we compared specifically our method with Gagnaire et al.’s and we tested the effect of the sampling bias, selection coefficient and percentage of loci under selection on the detection of outliers. A range of possible scenarios were run regarding: (i) different parental sampling sizes (3, 5, 10, 30, 50, 100, 300, 500 and 1000 individuals, from the original populations of 1000 individuals each) for a s = 99 [log (s + 1) = 2]; (ii) different values of the selection coefficient (-0.999, -0.990, -0.900, -0.684, 0.000, 2.162, 9.000, 99.000 and 999.000; equivalent to log (s + 1) values of -3.00, -2.00, -1.00, -0.50, -0.25, -0.10, -0.05, 0.00, 0.05, 0.10, 0.25, 0.50, 1.00, 2.00 and 3.00, respectively) with a parental sampling of 100 individuals each (from the original populations of 1000 individuals each); (iii) different proportions of selected loci from the 1000 simulated ones (0.0%, 0.5%, 1.0%, 5.0% and 10.0%). Every scenario was replicated 100 times.
3) Effect of the frequency estimation method
The original formulation of Gagnaire et al.’s and bal&gar-ca methods use the square-root method to estimate the null-allele frequency. As the allele frequency estimation is difficult in dominant markers, we investigated the effect on the outlier detection of using a Bayesian estimator of the null-allele frequency, , with non-uniform priors . For the bal&gar-ca method, we also modified the calculation of the frequencies of the parental allele confidence intervals, by using a quantile-based 95% probability interval based on the Bayesian estimator of the squared standard error, . To compare this Bayesian method and the previous approach for a particular common scenario, 900 independent loci plus 100 selected loci (s = 99) were simulated in 100 F1 hybrid individuals from two parental populations (A and B) with 100 individuals each. One hundred simulations were carried out to compare the results of both methods (Gagnaire et al.’s and bal&gar-ca) with both different allelic frequencies estimation (square-root and Bayesian).
In stable hybrid zones with several generations of hybrids, introgress seems to be a compromise solution. However, in transitory recent hybrid zones, bal&gar-ca and Gagnaire et al.’s methods are better options, depending on the size of the hybrid zone, our sampling strategy and the scope of the genome scan. Gagnaire et al.’s is useful when we have a good population sampling. However, when the sample sizes are modest and we want to avoid any possible false positive, bal&gar-ca is the advised method. Although Bayesian estimation of frequency allele has been proved to be useful in many cases, it is not advised for the bal&gar-ca method as it increases its type I error rate. Finally, none of these methods is advisable when the selection coefficient shows low values, which is not strange as, in these cases, long periods of time would be needed to accumulate detectable signal in the DNA .
Simulating hybridization with selection and genome scan for F1 individuals
In this example, we carry out the simulation of two parental populations of 100 individuals and 100 F1 hybrids for a total of 300 markers using the ‘hybridsim’ function. Positive selection was simulated with s = 10 for 15 out of 300 markers.
Firstly, we need to load AFLPsim and set the random seed number (arbitrarily to 123) for reproducibility.
> f1hybrid<-hybridsim(Nmarker=300, Na=100, Nb=100, Nf1=100, + type = 'selection', hybrid = 'F1', S=10, Nsel=15)
This is an object ‘hybridsim’ that contains the presence-absence matrices for the parentals (PA and PB) and for the hybrid classes (in this case, F1). In addition, this object contains the loci under selection (f1hybrid$SelMarkers) and the selection coefficient used in the simulation (f1hybrid$S).
M1 M2 M3 …
F1_1 0 1 0 …
F1_2 1 1 0 …
 13 26 46 55 69 78 149 158 161 177 183 229 230 289 290
> outlier<-gscan (f1hybrid, type = 'F1', method='bal&gar-ca')
> str (outlier)
List of 2
$ Pvalues :'data.frame': 300 obs. of 1 variable:
..$ fdrf1: num [1:300] 1 1 1 1 1 1 1 1 1 1 …
$ Outliers: num [1:10] 26 46 55 69 149 161 177 229 230 289
> plot.hybridsim(f1hybrid,hybrid = 'F1', + markers=outlier$Outliers)
In this example, we detected 10 out of 15 loci under selection (67% sensitivity) and we did not obtain any false positive.
Simulating demographical evolution under hybridization
Finally, we simulated hybridization on one area and evaluated its demographical consequences. For the initial frequencies we created a vector with the frequencies of Parental A, Parental B, F1, Backcross to Parental A, Backcross to Parental B and Fx. In our case, we fix Parental A and Parental B initial frequencies to 0.5.
> freqinit<-c (0.5,0.5,0,0,0,0)
Then, we create a matrix of assortative mating using the matrix function, and allow crosses between all taxa with the same probability.
> matingmat<-matrix (1,ncol=6,nrow=6)
In this example, parentals have similar fitness but F1 individuals’ is lower than parentals’. Here, we want to force asymmetrical introgression and breakdown occurs after F1 hybrids, with posterior hybrid generations (Fx) and backcrosses to Parental B being sterile, whereas backcrosses to Parental A (BxA) would have a similar fitness to F1 individuals. Hence, fitness would be modified as following:
> results<-demosimhybrid(freqinit, matingmat, fitness)
PA PB F1 BPA BPB Fx
G0 0.500 0.500 0.000 0.000 0 0
G1 0.333 0.333 0.333 0.000 0 0
G2 0.250 0.250 0.250 0.250 0 0
G3 0.500 0.167 0.167 0.167 0 0
G4 0.682 0.045 0.136 0.136 0 0
G5 0.838 0.003 0.040 0.120 0 0
G6 0.962 0.000 0.002 0.036 0 0
G7 0.998 0.000 0.000 0.002 0 0
G8 1.000 0.000 0.000 0.000 0 0
> plot.demosimhybrid (results)
This simulation study showed the interest of performing comparative studies in hybridization analytical software. Here we fill an important gap in this kind of software, as the R package AFLPsim application provides several useful tools in the context of hybridization studies. This is true specifically in relation to dominant markers with low sample sizes, in order to obtain markers under selection with a low rate of false positives and with a rather high sensitivity. None of the methods used is advisable when the selection coefficient shows low values. Moreover, AFLPsim provides a demographic method to study evolution in this context. Finally, we hold an on-going project to implement the bal&gar-ca method with biallelic codominant markers as well as single nucleotide polymorphisms (SNPs).
Availability and requirements
Project name: AFLPsim
Project home page: http://cran.r-project.org/web/packages/AFLPsim/
Operating system(s): Windows, Mac OS, Linux
Programming language: R
Other requirements: R version 2.15 or higher
License: GPL-2 | GPL-3
Any restrictions to use by non-academics: None
We thank Dr. M. Escudero for help in the ‘demosimhybrid’ code, for testing the software and providing feedback. Dr. O. Paun and two anonymous reviewers provided invaluable comments on a previous version of the manuscript. While working on this package, FB was partly funded by a Marie Curie Intra-European post-doctoral fellowship (‘TRANSADAPTATION’) at the Department of Systematic and Evolutionary Botany (University of Vienna) and Junta de Andalucía, Proyecto de Excelencia (2005/RNM484) and Ministerio de Educación y Ciencia, Flora iberica VIII (CGL2009-08178) and Flora iberica IX (CGL2012-32914), which were financed by FEDER funds.
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