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Figure 8 | Plant Methods

Figure 8

From: A rapid and versatile combined DNA/RNA extraction protocol and its application to the analysis of a novel DNA marker set polymorphic between Arabidopsis thaliana ecotypes Col-0 and Landsberg erecta

Figure 8

Sucrose Prep and the 'Touch-and-Go' methods works well in other plant species. DNA was isolated in duplicate using the Sucrose Prep or the 'Touch-and-Go' methods (indicated on the left) from leaves of one month old N. tabacum and N. benthamiana plants (upper two panels) or from leaves of 10 day-old barley plants (lower two panels). PCR was carried out for 40 cycles in a 50 μl reaction volume using primers for NtCDPK2 and NtRBCS in tobacco, and four different pairs of primers for barley. Only 10 μl of the final reaction was resolved by agarose (1%) gel electrophoresis for analysis.

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