Table 4 The response surface methodology (RSM) yield-vigor model, the artificial neural network(ANN) yield model, the vigor model, and the viable cell number model predictions were validated, and the results were considered credible with a relative error of less than 5%
Model | Cellulase R-10 content (%) | Macerozyme R-10 content (%) | Mannitol concentration(mol L−1) | Protoplast yield(× 106/100 mg) | Protoplast viability (%) | Number of viable cells(× 106/100 mg) | Relative error (%) | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
Predicted value | Experiment value | Predicted value | Experiment value | Predicted value | Experiment value | Protoplast yield | Protoplast viability | Number of viable cells | ||||
RSM yield-viability | 1.86 | 1.00 | 0.50 | 1.464 | 1.470 | 90.81 | 89.73 | 1.329 | 1.319 | 0.41 | 1.20 | 0.76 |
ANN-yield | 1.98 | 1.00 | 0.50 | 1.625 | 1.550 | 90.16 | 90.65 | 1.337 | 1.405 | 4.84 | 0.54 | 4.83 |
ANN-viability | 1.00 | 0.75 | 0.50 | 0.565 | 0.563 | 90.18 | 90.81 | 0.510 | 0.508 | 0.36 | 0.69 | 0.39 |
ANN-number of viable cells | 2.00 | 1.00 | 0.70 | 1.574 | 1.566 | 87.12 | 87.27 | 1.342 | 1.367 | 0.51 | 0.17 | 1.83 |