Table 1 Experimental results of three-factor, two-response-value Box-Behnken design test based on the preparation of protoplasts of Salsola laricifolia
Group | Cellulase R-10 content (%) | macerozyme R-10 content (%) | Mannitol concentration (mol·L−1) | Protoplast yield (106/100 mg) | Protoplast viability (%) | Number of viable cells (yield*vitality)(106/100 mg) |
|---|---|---|---|---|---|---|
1 | 1.00 | 0.50 | 0.60 | 0.575 | 85.78 | 0.493 |
2 | 3.00 | 0.50 | 0.60 | 0.256 | 85.58 | 0.219 |
3 | 1.00 | 1.00 | 0.60 | 0.683 | 88.02 | 0.601 |
4 | 3.00 | 1.00 | 0.60 | 0.800 | 78.69 | 0.630 |
5 | 1.00 | 0.75 | 0.50 | 0.563 | 90.81 | 0.511 |
6 | 3.00 | 0.75 | 0.50 | 0.383 | 84.65 | 0.324 |
7 | 1.00 | 0.75 | 0.70 | 0.583 | 87.62 | 0.511 |
8 | 3.00 | 0.75 | 0.70 | 0.615 | 84.27 | 0.518 |
9 | 2.00 | 0.50 | 0.50 | 1.125 | 90.10 | 1.013 |
10 | 2.00 | 1.00 | 0.50 | 1.460 | 90.62 | 1.323 |
11 | 2.00 | 0.50 | 0.70 | 1.320 | 89.23 | 1.178 |
12 | 2.00 | 1.00 | 0.70 | 1.566 | 87.27 | 1.367 |
13 | 2.00 | 0.75 | 0.60 | 0.955 | 89.63 | 0.856 |
14 | 2.00 | 0.75 | 0.60 | 0.945 | 89.42 | 0.845 |
15 | 2.00 | 0.75 | 0.60 | 1.035 | 88.25 | 0.913 |
16 | 2.00 | 0.75 | 0.60 | 1.090 | 88.57 | 0.965 |
17 | 2.00 | 0.75 | 0.60 | 1.010 | 87.21 | 0.881 |