Fig. 5From: Optimization of preparation conditions for Salsola laricifolia protoplasts using response surface methodology and artificial neural network modelingResults of the preparation of protoplasts of Salsola laricifolia under the optimal preparation conditions. To enhance visualization of protoplasts, a and b were captured using blood counting plates (25 × 16) as a background under a microscope (10 × 40). Additionally, the protoplast concentrations in b were diluted by a factor of twoBack to article page