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Fig. 5 | Plant Methods

Fig. 5

From: Optimization of preparation conditions for Salsola laricifolia protoplasts using response surface methodology and artificial neural network modeling

Fig. 5

Results of the preparation of protoplasts of Salsola laricifolia under the optimal preparation conditions. To enhance visualization of protoplasts, a and b were captured using blood counting plates (25 × 16) as a background under a microscope (10 × 40). Additionally, the protoplast concentrations in b were diluted by a factor of two

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