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Fig. 1 | Plant Methods

Fig. 1

From: Cryopreservation of sessile oak (Quercus petraea (Matt.) Liebl.) plumules using aluminium cryo-plates: influence of cryoprotection and drying

Fig. 1

Cryopreservation of Q. petraea plumules and their in vitro regrowth. Acorns from which embryonic axes were isolated (A), embryonic axes from which plumules were isolated (B), an aluminium plate with plumules placed in 3 mm wells capsulated in calcium alginate (beads) - cryoprotection and desiccation stage (C), a surviving plumule after storage in liquid nitrogen, 1 week of in vitro culture (D), after 2 weeks of in vitro culture – regrowth of cryopreserved plumules (E), and 3-month-old shoots with leaves derived from plumules after cryopreservation (F)

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