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Fig. 8 | Plant Methods

Fig. 8

From: Novel in silico screening system for plant defense activators using deep learning-based prediction of reactive oxygen species accumulation

Fig. 8

Verification of potential ROS modulators selected on flg22 peptide-inducible ROS production in Arabidopsis seedlings. a The effect of potential ROS modulators on flg22 peptide-inducible ROS production in Arabidopsis seedlings. A seedling was placed into each well of a 96-well white plate, and each compound (final concentration 100 or 50 µM) was added to the indicated wells for 24 h. ROS indicator L-012 was added to the wells and then flg22-peptide elicitor (final concentration 1 µM) was added to the wells to induce ROS production. ROS-dependent chemiluminescence was recorded using a multi-luminometer. Selected criteria are shown in red line. b The vertical axis represents the peak height of ROS-dependent chemiluminescence levels in Arabidopsis seedlings triggered by flg22 peptide (1 µM), and the horizontal axis represents the chemical compounds arranged in ascending order of their ROS-dependent chemiluminescence levels

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Plant Methods

ISSN: 1746-4811