Fig. 7
Verification of potential ROS modulators on cryptogein-inducible ROS production in tobacco BY-2 cultured cells. a The effect of potential ROS modulators on cryptogein-inducible ROS production in tobacco BY-2 cultured cells. Cultured cells were dispensed into each well of a 96-well white plate, and each compound (final concentration 100 or 50 µM) was added to the indicated wells for 1.5 h. Next, cryptogein (final concentration 25 or 50 nM) was added to the wells and incubated for 3 h. After cryptogein treatment, L-012 was added directly to the wells and ROS-dependent chemiluminescence was recorded using a multi-luminometer. DMSO (1%) was used as a solvent control. Error bars represent SE (n = 3). a < 0.05, b < 0.01, c < 0.005; significantly different from the DMSO control. b The vertical axis represents the logarithmic conversion of ROS-dependent chemiluminescence levels in tobacco BY-2 cultured cells triggered by cryptogein (25 nM), and the horizontal axis represents the chemical compounds arranged in ascending order based on their ROS-dependent chemiluminescence levels. Selected criteria (log10[4.67]) are shown in red line