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Fig. 7 | Plant Methods

Fig. 7

From: A protocol for a turbidimetric assay using a Saccharomyces cerevisiae thiamin biosynthesis mutant to estimate total vitamin B1 content in plant tissue samples

Fig. 7

Picture of a standard series 24-well plate and check-up for yeast culture contamination. A 24-well plate used for the assay standard series curve is depicted after 17 h of growth (A). In this plate, each standard concentration (C1–C12) is included in duplicate. With the naked eye, increasing turbidity from C1 to C12 is visible. Yeast cultures can be spotted (30 μl) on solidified TFYM (TFYM medium + 1% ultrapure agarose) plates to test for contamination (B). WT (Wild Type; BY4741) yeast depicts clear growth on TFYM, while thi6 shows no growth. Contamination, by inoculating the thi6 strain with 1/100 (10–2) or 1/10.000 (10–4) dilution of WT, shows that absence of prototrophic cells can be verified easily

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Plant Methods

ISSN: 1746-4811