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Fig. 6 | Plant Methods

Fig. 6

From: A protocol for a turbidimetric assay using a Saccharomyces cerevisiae thiamin biosynthesis mutant to estimate total vitamin B1 content in plant tissue samples

Fig. 6

Flowchart of yeast microbiological assay for thiamin determination. The green frame represents the preparation of the plant samples. The blue frame shows the 12 concentrations (C1 to C12) of the thiamin standard in nM. The red frame covers the preparation of the yeast cultures. YPD, yeast extract-peptone-dextrose medium; TFYM, Thiamin-free yeast medium (ForMedium: CYN4701 + ForMedium: DCS0011 + 1% sucrose); OD, optical density

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Plant Methods

ISSN: 1746-4811