Fig. 2
Factors influencing yeast growth during assays. The thiamin concentration-responsive growth of the thi6 Saccharomyces cerevisiae mutant strain, used for turbidimetric assay of vitB1 quantification [28], was examined upon usage of fresh or stored yeast cultures (A) or higher nutrient composition of the TFYM growth medium (B). Following the assay protocol, Optical Density (OD) was measured after 17 h of growth. A Here, however, small adaptations to the protocol were tested, as stored, washed yeast cultures of different age (stored at 4 °C for 1, 3 or 4 months) were utilized to test their thiamin concentration-responsiveness. Note that the cultures were tested at a similar time, meaning that these originated from different thi6 cultures separately washed (not one culture tested at different timepoints). B Similarly, thi6 growth was followed upon growth in double concentration TFYM, to examine whether higher nutrient composition can have an effect on the assay outcome. Error bars represent standard deviation as measured from 4 technical replicates